Rh2CG274000

Chromatin assembly factor 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011488
Physical Location & Seq
Reverse (-)
30086083 .. 30092145
6063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG274000.1

Sequence Viewer

Length: 1698 bp
ATGAAAGGCGGGACGGTCCAGATCAACTGGCACGACACCAAGCCCGTCCTAACCCTAGATTTCCACCCTCATTCCGGCATTCTCGCCACCGGCGGCGCCGATTACGACATCAAGCTTTGGTTGATAAATTCCGGCGAGAAGCTCCCTTCAGTGTCGTATCATAATAGCCTTTCTTACCATAGTTCTGCTATAAATGTCCTTCGCTTCTCTCCTTCTGGAGAACAAATTGCCTCCGGTGCAGATGGAGGCGAGCTGATTATATGGAAGTTGCATGCAACAGAAAATAGTCAAACGTGGAAGGTTCTTAAGACATTGTCATTTCACCGGAAAGACGTGCTGGACCTCGAGTGGTCTGCTGATGGTTCATTTCTCATCTCCGGTTCAGTCGATAATTCATGCATCATATGGGATGCTAACAAAGGTTCTGTCCATCAGATTTTGGATTCCCATTTCCATTATGTTCAAGGTGTGGCATGGGACCCATTGGCCAAGTATGTTGCCTCCCTTAGTTCAGATAGAACTTGTCGCATTTATCTTAAAAAGGCTCAAACAAAAACCAAGGGTGCCGATAAGACAAATTATGTTTGTCAACATGTTATTTCAAAGGCCGAAAACCCTGCAGATGAGTCTAAGTCTGCGAAAAACCATCTCTTTCACGATGAGACACTACCATCTTTTTTCCGGAGGTTAGCGTGGTCACCTGATGGATCATTTCTACTTGTTCCAGCAGGTTCCTACAAAGTATCACCTGCAACAGAAACAATAAATACTGCTTATGTATTTTCAAGAAAGGACCTTTCAAGGCCTGCTCTACATCTCCCTTGCGCCAGCAAACCAATAGTAGCAGTTCGATTCTGCCCTTTGGCTTTTAGACTGCGGGGAACCAATCCATCGGGGTTCTTTAAGCTTCCCCATCGTATAGTTTTTGCGGTGGCCACTTTAGATTCTCTGAACATTTATGACACAGAGAGTGTTACTCCAATTGCAGTTTTGGCTGGTCTTCATTATGCAGCCATAACAGACATCACATGGTCACCTGATGCCCATTATCTAGCATTATCTTCCCAAGATGGTTACTGCACTTTAGTAGAATTTGAAAATGATGAACTGGGATCACCCATCTATTTGTCAGAAGAGAAGAAAGTCATTGGTGAAGGGGATGAAAGCTTGGTAAAGAATCCAGAGGGCATGTTAATTGAAGCTACTACAGAAAATAGTCTTGCTGCAACTAAAACCATTAATGAAGAGAACAAAATCTCAGTTAAGAAGCCAGACGACATGGTAATTGACACTACTCCAAGTAAAAGTCTAGTTGCAGCCGATAATGAAATATCAGAAGCAGAAAGGAATAAGCAAACAGAAGCAGAAAGGAATAAGCAAACAGAAGCAGAAAAGAAAGTCATACGTGATGACAGAAGCAGAGTTGAGAAGCCAGAGGGCATGGTAATTGACACTACCACAAATGATAGTCTTGCAGCCGATATTGAAATATCAGAAGCAGAAAGGAAAGAAAAGAAAGTCATGGGTGATGACAGTAGCACAGTTCAGAAGCCAGAGGACATGGTAATTGAAGGTTCAGCAAATGATAGTCTTGTAGCAGCAAACGATGGCAAAAAAAAATCACTGAGCACAGTGACGACTCCTACTGTAAATAAGCCTACTAAAAGGCGCATTACACCCATGGCAATTGACCCTTGA

Protein Analysis

565

Amino Acids

62.16

Weight (kDa)

5.76

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 1 - 370 3.7e-117 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 9 - 181 3.6e-08 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 11 - 93 9.3e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 12 - 101 1.4e-09 THOC3 beta-propeller domain
Beta-prop_EML PF23409 12 - 145 1.1e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 13 - 184 3.6e-28 WDR5 beta-propeller domain
Beta-prop_IFT140_1st PF23383 13 - 93 4.2e-07 IFT140 first beta-propeller
WD40_Prp19 PF24814 13 - 101 1.3e-09 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 13 - 187 3.9e-12 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 13 - 159 9.3e-14 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 14 - 132 1.3e-09 WDR3 second beta-propeller domain
EIF3I PF24805 16 - 184 2.8e-11 EIF3I
Beta-prop_EML_2 PF23414 18 - 185 6.8e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR75_1st PF23869 19 - 95 9.9e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 27 - 184 1.8e-07 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 60 - 202 7e-08 MABP1/WDR62 second WD40 domain
WDR55 PF24796 62 - 148 1.3e-08 WDR55
Beta-prop_IFT122_1st PF23381 63 - 191 1.9e-06 IFT122 first beta-propeller
WD40_CDC20-Fz PF24807 96 - 204 5.2e-12 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 97 - 184 7.5e-12 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 97 - 196 5.3e-12 WDR3 first beta-propeller domain
WD40 PF00400 99 - 137 3e-08 WD domain, G-beta repeat
WD40_Gbeta PF25391 104 - 196 1.5e-07 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 108 - 193 6.2e-14 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 112 - 245 1.8e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64630 AT5G64630 AT5G64630
fragaria_vesca FvH4_1g24150 FvH4_1g24150 FvH4_1g24150 FvH4_1g24150
malus_domestica MD01G1002400.v1.1
prunus_persica Prupe.6G159400_v2.0.a1
pyrus_communis pycom01g02500
rosa_chinensis RchiOBHm_Chr2g0120381
rosa_laevigata RLG00000018554
rosa_multiflora Rmu_sc0005082.1_g000015
rosa_roxburghii Rroxscaffold_2G00123450
rosa_rugosa Rorug02G0229200
rosa_samantha Rh2AG285200 Rh2BG296300 Rh2CG274000 Rh2DG310200
rosa_wichuraiana Rw2G022890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 757
Acc36I ACCTGC 2 cut(s) 719, 757
AccB1I GGYRCC 2 cut(s) 95, 563
AccIII TCCGGA 1 cut(s) 681
AciI CCGC 4 cut(s) 9, 93, 877, 929
AclWI GGATC 2 cut(s) 715, 1120
AcoI YGGCCR 2 cut(s) 486, 933
AcsI RAATTY 2 cut(s) 127, 1091
AcuI CTGAAG 1 cut(s) 132
AcyI GRCGYC 1 cut(s) 96
AfiI CCNNNNNNNGG 1 cut(s) 74
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 8 cut(s) 464, 603, 786, 801, 1097, 1199, 1487, 1571
AjiI CACGTC 1 cut(s) 334
AluBI AGCT 6 cut(s) 115, 142, 253, 907, 1167, 1202
AluI AGCT 6 cut(s) 115, 142, 253, 907, 1167, 1202
Alw21I GWGCWC 1 cut(s) 1631
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 2 cut(s) 715, 1120
Ama87I CYCGRG 1 cut(s) 344
Aor13HI TCCGGA 1 cut(s) 681
AoxI GGCC 4 cut(s) 486, 606, 803, 933
ApeKI GCWGC 5 cut(s) 1010, 1223, 1316, 1475, 1598
ApoI RAATTY 2 cut(s) 127, 1091
ArsI GACNNNNNNTTYG 2 cut(s) 411, 443
AseI ATTAAT 1 cut(s) 1239
AspLEI GCGC 3 cut(s) 98, 827, 1671
AspS9I GGNCC 4 cut(s) 16, 340, 478, 793
AsuHPI GGTGA 7 cut(s) 314, 690, 738, 1026, 1107, 1163, 1538
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 4 cut(s) 16, 340, 478, 793
BalI TGGCCA 2 cut(s) 488, 935
BanI GGYRCC 2 cut(s) 95, 563
BbsI GAAGAC 1 cut(s) 992
Bbv12I GWGCWC 1 cut(s) 1631
BbvI GCAGC 5 cut(s) 1022, 1210, 1328, 1487, 1610
BcgI CGANNNNNNTGC 4 cut(s) 335, 369, 599, 633
BcoDI GTCTC 1 cut(s) 656
BfaI CTAG 3 cut(s) 56, 1052, 1310
BfmI CTRYAG 2 cut(s) 618, 1206
BfoI RGCGCY 1 cut(s) 99
BfrI CTTAAG 1 cut(s) 305
BfuAI ACCTGC 2 cut(s) 719, 757
BisI GCNGC 6 cut(s) 94, 1011, 1224, 1317, 1476, 1599
BlsI GCNGC 6 cut(s) 95, 1012, 1225, 1318, 1477, 1600
Bme18I GGWCC 4 cut(s) 16, 340, 478, 793
BmeT110I CYCGRG 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 4 cut(s) 16, 340, 478, 793
BmiI GGNNCC 6 cut(s) 97, 479, 480, 565, 733, 883
BmrI ACTGGG 1 cut(s) 1118
BmsI GCATC 3 cut(s) 400, 408, 1030
BmuI ACTGGG 1 cut(s) 1118
BpiI GAAGAC 1 cut(s) 992
BplI GAGNNNNNCTC 2 cut(s) 961, 993
BpmI CTGGAG 1 cut(s) 237
BsaAI YACGTR 1 cut(s) 1406
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 558, 1680
BsaWI WCCGGW 4 cut(s) 233, 324, 377, 681
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 2 cut(s) 32, 1113
BseAI TCCGGA 1 cut(s) 681
BseDI CCNNGG 2 cut(s) 558, 1680
BseGI GGATG 2 cut(s) 415, 1165
BseLI CCNNNNNNNGG 1 cut(s) 74
BseMII CTCAG 2 cut(s) 1272, 1616
BseNI ACTGG 2 cut(s) 32, 1113
BseXI GCAGC 5 cut(s) 1022, 1210, 1328, 1487, 1610
BsgI GTGCAG 2 cut(s) 258, 1063
BshFI GGCC 4 cut(s) 488, 608, 805, 935
BshNI GGYRCC 2 cut(s) 95, 563
BsiHKAI GWGCWC 1 cut(s) 1631
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 7 cut(s) 75, 90, 132, 234, 325, 378, 682
BslFI GGGAC 2 cut(s) 25, 491
BslI CCNNNNNNNGG 1 cut(s) 74
BsmAI GTCTC 1 cut(s) 656
BsmFI GGGAC 2 cut(s) 25, 491
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 4 cut(s) 488, 608, 805, 935
BsoBI CYCGRG 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 1631
Bsp13I TCCGGA 1 cut(s) 681
Bsp143I GATC 3 cut(s) 21, 707, 1112
Bsp19I CCATGG 1 cut(s) 1680
BspACI CCGC 4 cut(s) 9, 93, 877, 929
BspANI GGCC 4 cut(s) 488, 608, 805, 935
BspCNI CTCAG 2 cut(s) 1271, 1617
BspEI TCCGGA 1 cut(s) 681
BspLI GGNNCC 6 cut(s) 97, 479, 480, 565, 733, 883
BspMAI CTGCAG 1 cut(s) 622
BspMI ACCTGC 2 cut(s) 719, 757
BspPI GGATC 2 cut(s) 715, 1120
BspT107I GGYRCC 2 cut(s) 95, 563
BspTI CTTAAG 1 cut(s) 305
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 2 cut(s) 32, 1113
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 558, 1680
BssMI GATC 3 cut(s) 21, 707, 1112
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 558, 1680
Bst4CI ACNGT 5 cut(s) 16, 1535, 1543, 1633, 1648
Bst6I CTCTTC 2 cut(s) 1128, 1239
BstACI GRCGYC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 305
BstBAI YACGTR 1 cut(s) 1406
BstC8I GCNNGC 4 cut(s) 251, 273, 807, 829
BstDEI CTNAG 4 cut(s) 506, 630, 1258, 1625
BstDSI CCRYGG 1 cut(s) 1680
BstEII GGTNACC 2 cut(s) 696, 1032
BstF5I GGATG 2 cut(s) 415, 1165
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 3 cut(s) 98, 827, 1671
BstKTI GATC 3 cut(s) 24, 710, 1115
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 3 cut(s) 21, 707, 1112
BstMWI GCNNNNNNNGC 2 cut(s) 236, 992
BstNSI RCATGY 3 cut(s) 275, 596, 1192
BstPI GGTNACC 2 cut(s) 696, 1032
BstSFI CTRYAG 2 cut(s) 618, 1206
BstV1I GCAGC 5 cut(s) 1022, 1210, 1328, 1487, 1610
BstV2I GAAGAC 1 cut(s) 992
BsuRI GGCC 4 cut(s) 488, 608, 805, 935
BtgI CCRYGG 1 cut(s) 1680
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 2 cut(s) 415, 1165
BtsIMutI CAGTG 3 cut(s) 156, 1622, 1638
BveI ACCTGC 2 cut(s) 719, 757
Cac8I GCNNGC 4 cut(s) 251, 273, 807, 829
CfoI GCGC 3 cut(s) 98, 827, 1671
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 4 cut(s) 16, 340, 478, 793
DdeI CTNAG 4 cut(s) 506, 630, 1258, 1625
DinI GGCGCC 1 cut(s) 97
DpnI GATC 3 cut(s) 23, 709, 1114
DpnII GATC 3 cut(s) 21, 707, 1112
EaeI YGGCCR 2 cut(s) 486, 933
Eam1104I CTCTTC 2 cut(s) 1128, 1239
EarI CTCTTC 2 cut(s) 1128, 1239
Eco130I CCWWGG 2 cut(s) 558, 1680
Eco147I AGGCCT 1 cut(s) 805
Eco47I GGWCC 4 cut(s) 16, 340, 478, 793
Eco57I CTGAAG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 344
Eco91I GGTNACC 2 cut(s) 696, 1032
EcoO109I RGGNCCY 2 cut(s) 478, 793
EcoO65I GGTNACC 2 cut(s) 696, 1032
EcoT14I CCWWGG 2 cut(s) 558, 1680
EcoT22I ATGCAT 1 cut(s) 401
EgeI GGCGCC 1 cut(s) 97
EheI GGCGCC 1 cut(s) 97
ErhI CCWWGG 2 cut(s) 558, 1680
FaqI GGGAC 2 cut(s) 25, 491
FauI CCCGC 2 cut(s) 2, 870
FauNDI CATATG 1 cut(s) 404
Fnu4HI GCNGC 6 cut(s) 94, 1011, 1224, 1317, 1476, 1599
FokI GGATG 2 cut(s) 422, 1172
Fsp4HI GCNGC 6 cut(s) 94, 1011, 1224, 1317, 1476, 1599
FspBI CTAG 3 cut(s) 56, 1052, 1310
GlaI GCGC 3 cut(s) 97, 826, 1670
GluI GCNGC 6 cut(s) 94, 1011, 1224, 1317, 1476, 1599
GsuI CTGGAG 1 cut(s) 237
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 4 cut(s) 488, 608, 805, 935
HapII CCGG 7 cut(s) 75, 90, 132, 234, 325, 378, 682
HhaI GCGC 3 cut(s) 98, 827, 1671
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 3 cut(s) 96, 825, 1669
HinP1I GCGC 3 cut(s) 96, 825, 1669
HincII GTYRAC 1 cut(s) 590
HindII GTYRAC 1 cut(s) 590
HindIII AAGCTT 3 cut(s) 113, 905, 1165
HinfI GANTC 6 cut(s) 443, 626, 852, 944, 1177, 1639
HpaII CCGG 7 cut(s) 75, 90, 132, 234, 325, 378, 682
HphI GGTGA 7 cut(s) 314, 690, 738, 1026, 1107, 1163, 1538
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 7 cut(s) 435, 514, 951, 1132, 1336, 1495, 1548
Hpy188III TCNNGA 6 cut(s) 19, 216, 656, 682, 786, 1181
Hpy8I GTNNAC 1 cut(s) 590
HpyAV CCTTC 6 cut(s) 156, 209, 222, 292, 1148, 1565
HpyCH4III ACNGT 5 cut(s) 16, 1535, 1543, 1633, 1648
HpyCH4IV ACGT 3 cut(s) 293, 333, 1405
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 992
HpyF3I CTNAG 4 cut(s) 506, 630, 1258, 1625
HpySE526I ACGT 3 cut(s) 293, 333, 1405
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 3 cut(s) 96, 825, 1669
KasI GGCGCC 1 cut(s) 95
KflI GGGWCCC 1 cut(s) 478
Kpn2I TCCGGA 1 cut(s) 681
Kzo9I GATC 3 cut(s) 21, 707, 1112
LmnI GCTCC 1 cut(s) 147
Lsp1109I GCAGC 5 cut(s) 1022, 1210, 1328, 1487, 1610
LweI GCATC 3 cut(s) 400, 408, 1030
MaeI CTAG 3 cut(s) 56, 1052, 1310
MaeII ACGT 3 cut(s) 293, 333, 1405
MaeIII GTNAC 5 cut(s) 696, 973, 1032, 1073, 1633
MalI GATC 3 cut(s) 23, 709, 1114
MboI GATC 3 cut(s) 21, 707, 1112
MboII GAAGA 5 cut(s) 992, 1053, 1145, 1150, 1256
MfeI CAATTG 2 cut(s) 981, 1686
MhlI GDGCHC 1 cut(s) 1631
MlsI TGGCCA 2 cut(s) 488, 935
MluNI TGGCCA 2 cut(s) 488, 935
Mly113I GGCGCC 1 cut(s) 96
MlyI GAGTC 2 cut(s) 635, 1633
MnlI CCTC 9 cut(s) 78, 239, 241, 353, 511, 678, 1177, 1429, 1549
Mox20I TGGCCA 2 cut(s) 488, 935
Mph1103I ATGCAT 1 cut(s) 401
MroI TCCGGA 1 cut(s) 681
MscI TGGCCA 2 cut(s) 488, 935
MseI TTAA 6 cut(s) 306, 537, 903, 1193, 1239, 1263
Msp20I TGGCCA 2 cut(s) 488, 935
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 7 cut(s) 75, 90, 132, 234, 325, 378, 682
MunI CAATTG 2 cut(s) 981, 1686
Mva1269I GAATGC 1 cut(s) 78
MwoI GCNNNNNNNGC 2 cut(s) 236, 992
NarI GGCGCC 1 cut(s) 96
NcoI CCATGG 1 cut(s) 1680
NdeI CATATG 1 cut(s) 404
NdeII GATC 3 cut(s) 21, 707, 1112
NlaIV GGNNCC 6 cut(s) 97, 479, 480, 565, 733, 883
NmuCI GTSAC 3 cut(s) 696, 1032, 1633
NsiI ATGCAT 1 cut(s) 401
NspI RCATGY 3 cut(s) 275, 596, 1192
PaeI GCATGC 1 cut(s) 275
PaeR7I CTCGAG 1 cut(s) 344
PaqCI CACCTGC 1 cut(s) 757
PceI AGGCCT 1 cut(s) 805
PciI ACATGT 1 cut(s) 592
PctI GAATGC 1 cut(s) 78
PfeI GAWTC 4 cut(s) 443, 852, 944, 1177
PflFI GACNNNGTC 1 cut(s) 313
PkrI GCNGC 6 cut(s) 95, 1012, 1225, 1318, 1477, 1600
PleI GAGTC 2 cut(s) 634, 1633
PluTI GGCGCC 1 cut(s) 99
PpsI GAGTC 2 cut(s) 634, 1633
Ppu21I YACGTR 1 cut(s) 1406
PpuMI RGGWCCY 2 cut(s) 478, 793
PscI ACATGT 1 cut(s) 592
PshBI ATTAAT 1 cut(s) 1239
Psp5II RGGWCCY 2 cut(s) 478, 793
PspEI GGTNACC 2 cut(s) 696, 1032
PspN4I GGNNCC 6 cut(s) 97, 479, 480, 565, 733, 883
PspPI GGNCC 4 cut(s) 16, 340, 478, 793
PspPPI RGGWCCY 2 cut(s) 478, 793
PspXI VCTCGAGB 1 cut(s) 344
PstI CTGCAG 1 cut(s) 622
PsyI GACNNNGTC 1 cut(s) 313
SaqAI TTAA 6 cut(s) 306, 537, 903, 1193, 1239, 1263
SatI GCNGC 6 cut(s) 94, 1011, 1224, 1317, 1476, 1599
Sau3AI GATC 3 cut(s) 21, 707, 1112
Sau96I GGNCC 4 cut(s) 16, 340, 478, 793
SchI GAGTC 2 cut(s) 635, 1633
SduI GDGCHC 1 cut(s) 1631
SfaNI GCATC 3 cut(s) 400, 408, 1030
SfcI CTRYAG 2 cut(s) 618, 1206
SfoI GGCGCC 1 cut(s) 97
Sfr274I CTCGAG 1 cut(s) 344
SgrAI CRCCGGYG 1 cut(s) 89
SinI GGWCC 4 cut(s) 16, 340, 478, 793
SlaI CTCGAG 1 cut(s) 344
SmlI CTYRAG 2 cut(s) 305, 344
SmoI CTYRAG 2 cut(s) 305, 344
SphI GCATGC 1 cut(s) 275
SseBI AGGCCT 1 cut(s) 805
SsiI CCGC 4 cut(s) 9, 93, 877, 929
SspDI GGCGCC 1 cut(s) 95
SspMI CTAG 3 cut(s) 56, 1052, 1310
StuI AGGCCT 1 cut(s) 805
StyI CCWWGG 2 cut(s) 558, 1680
TaaI ACNGT 5 cut(s) 16, 1535, 1543, 1633, 1648
TaiI ACGT 3 cut(s) 296, 336, 1408
TaqI TCGA 3 cut(s) 345, 387, 850
TauI GCSGC 1 cut(s) 96
TfiI GAWTC 4 cut(s) 443, 852, 944, 1177
Tru1I TTAA 6 cut(s) 306, 537, 903, 1193, 1239, 1263
Tru9I TTAA 6 cut(s) 306, 537, 903, 1193, 1239, 1263
TscAI CASTG 3 cut(s) 156, 1629, 1638
TseFI GTSAC 3 cut(s) 696, 1032, 1633
TseI GCWGC 5 cut(s) 1010, 1223, 1316, 1475, 1598
Tsp45I GTSAC 3 cut(s) 696, 1032, 1633
TspDTI ATGAA 8 cut(s) 17, 354, 384, 992, 1119, 1176, 1257, 1341
TspRI CASTG 3 cut(s) 156, 1629, 1638
Tth111I GACNNNGTC 1 cut(s) 313
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 4 cut(s) 16, 340, 478, 793
VspI ATTAAT 1 cut(s) 1239
XapI RAATTY 2 cut(s) 127, 1091
XceI RCATGY 3 cut(s) 275, 596, 1192
XhoI CTCGAG 1 cut(s) 344
XspI CTAG 3 cut(s) 56, 1052, 1310
Zsp2I ATGCAT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.