Rh2AG372300

PX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008247
Physical Location & Seq
Reverse (-)
55254505 .. 55399791
145287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG372300.1

Sequence Viewer

Length: 1899 bp
ATGAGCATGCCTGCTGCCTTTTTATTTATTTGCAACCATAACTACGATAGTTTCGCTATTAACATTTACAGAGAACAGTTTTATAGGGTTCAAGTTGGTCTACAATCACCGGAAGGAATCACAACCACTCGAGGGATATTACGAAGATTTAATGATTTTCTAAAGCTATTTTCTGAACTTAAAAAGGCATTCCCCCTGAAAGATCTTCCTCCAGCTCCTCCAAAGGGGTTTCTGAGAATGAAAAGTCGGATACTATTGGAAGAGCGAAGGTGTTCTTTGGAGGATTGGATAGAAAAGCTGTTATCAGATATCGATATATCAAGAAGTGTGTTGGTTGCAACCTTTCTAGAGCTGGAAGCTGCTGCGAGGTCTTCATTCAATGATCCGCAGCTAATTTCAGATGTAAACTCAACAAGTGGCACAGCTCCGTCTTCTTTGTTACAAACCCAATCAGATCTCTCTGCTGTTGCTGGTAGCTCATCTGTCACATCAGATAATGGTAATGATACTCCATACGAGGTATCTGAGCTTGGAACACCAAGGTATGGAGATGATAGCTTTGCTAATCTTGCCATGGAGCTTTCGACATCTGATCAGGACTTTGATGATTCGATAGATACATCTGTTAAGGTTGGCCTATTTAACCAGAAATTTGTTCGGGGAAGCCTAGAAAGTCGCTCCAGGCGCCAAATTTCTGATAGAAGTAATGCGAATACTATAGACAAGGAGCAAACAAGTGAAAATACCTATGGTTCTAAACCTCTTTGCATGGATGGAACCGAGTTCTTTGTAGAACCTGAGGAAAGGAAGAGGGATGGCCATGCTCAAGGACCATCAACAGAGAGTTTTGGAAGCGATACTTCTACCTTGCGGGTGGGCAATTTATGTGGTGATGGCTCCCTCAACCTTCCTGAAAGTTCTGAAGCTCCTACAATTGCAGATACTCTTGACAACTCAGATTTGAACTTTTCAAGGGCTATACTAGCTGCTTTTCCACATGATGAGCGACAAAAATTAAACAGAGTTCTGATTACATTGCAACAGAGATTAGGAACAGCAAAAATTGACATCGAGGATCTTATAGCAAGATTGAATCAAGAAGTTGCTGTGAGACAATTTCTCACAACAAAGGTTAAAGATCTGGAAGTGGAACTTGAAACTACCCAGCAAAATTGTAAAGAAAATATTCAGCAGGCTGTCTTAAATGAAAAAGAAAGATTTACCCAAATGCAGTGGGATATGGAGGAACTGCGAAGGAAGTGCTTGGACTTGGAATTACAATTGAGATCTGAGCAGGATGCAAGGGAGCACTCGGAGTCAACAAAAATTTCTATCATTCAGGAGAATGACATATTGATACAGGAGTTGGATGTCTGTAGGAGGCAGCTTGACAACTTGCAGAAATTTCATGAAGAGTTTGAGGTGAAATCAAAGACAGATGTAAAGCTACTTGTTAAAGAGGTCAAATCTCTTCGAAATTCACAATCAGAACTGAAGCAGGAACTCAGTCGATTGATGAAGGAAAAATTAGAAGTAGAGAGAACTCTTCAAAAAGAAAAGCGAAGAATAGAGTGTGCAGCAACTACTAACGCAAAGCTGCTGCATGAATGTGAAATACTTCGCAGTCGGCTTCAAGAATGTAGTGTCAATTTTCTGGTTGAAGAGGAAGATAAACTGATCTTGGATACTTCATCACCATCTGATGCTATTGATCTACTGACAACGTCTGACAATCGAATTGGTCTTCTTCTTGCAGAGGCACAGCTCCTTGCACAGGATGTCGAAAGTGCTGTTATAGCAGTGGACGATGCTCAAGACATCAAGGGTGGTGATAGGATGGCTGATGATGAGCTGAGGAAGATGCTGACAGATCAGTTATTGTTGACAATGCCAGACTAA

Protein Analysis

632

Amino Acids

71.46

Weight (kDa)

4.78

Isoelectric Point (pI)

57.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PX PF00787 44 - 117 1.5e-12 PX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 684
AccB7I CCANNNNNTGG 1 cut(s) 545
AccI GTMKAC 1 cut(s) 100
AciI CCGC 2 cut(s) 386, 871
AclWI GGATC 2 cut(s) 377, 1083
AcoI YGGCCR 1 cut(s) 817
AcsI RAATTY 5 cut(s) 650, 690, 1326, 1403, 1477
AcuI CTGAAG 2 cut(s) 942, 1514
AcyI GRCGYC 1 cut(s) 685
AfiI CCNNNNNNNGG 4 cut(s) 132, 224, 545, 1774
AgsI TTSAA 9 cut(s) 92, 379, 964, 972, 1093, 1157, 1550, 1634, 1661
AjnI CCWGG 1 cut(s) 680
Alw21I GWGCWC 1 cut(s) 1311
Alw26I GTCTC 1 cut(s) 1105
AlwI GGATC 2 cut(s) 377, 1083
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 2 cut(s) 634, 817
ApeKI GCWGC 9 cut(s) 14, 359, 362, 388, 986, 1384, 1577, 1597, 1600
ApoI RAATTY 5 cut(s) 650, 690, 1326, 1403, 1477
Asp700I GAANNNNTTC 3 cut(s) 271, 1185, 1617
AspLEI GCGC 1 cut(s) 687
AspS9I GGNCC 1 cut(s) 830
AsuHPI GGTGA 5 cut(s) 99, 902, 1435, 1686, 1841
AsuII TTCGAA 1 cut(s) 1474
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 1 cut(s) 830
AxyI CCTNAGG 1 cut(s) 798
BaeI ACNNNNGTAYC 4 cut(s) 609, 642, 1349, 1382
BalI TGGCCA 1 cut(s) 819
BanI GGYRCC 1 cut(s) 684
BbsI GAAGAC 3 cut(s) 363, 423, 1736
Bbv12I GWGCWC 1 cut(s) 1311
BbvCI CCTCAGC 1 cut(s) 1853
BbvI GCAGC 8 cut(s) 346, 349, 400, 973, 1396, 1584, 1587, 1589
BccI CCATC 6 cut(s) 767, 809, 841, 887, 1705, 1831
BcgI CGANNNNNNTGC 2 cut(s) 1242, 1276
BciT130I CCWGG 1 cut(s) 682
BciVI GTATCC 2 cut(s) 243, 1678
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 1 cut(s) 1105
BfaI CTAG 3 cut(s) 347, 668, 983
BfmI CTRYAG 2 cut(s) 717, 1375
BfoI RGCGCY 1 cut(s) 688
BfuI GTATCC 2 cut(s) 243, 1678
BglII AGATCT 4 cut(s) 202, 454, 1138, 1286
BisI GCNGC 9 cut(s) 15, 360, 363, 389, 987, 1385, 1578, 1598, 1601
BlsI GCNGC 9 cut(s) 16, 361, 364, 390, 988, 1386, 1579, 1599, 1602
Bme1390I CCNGG 1 cut(s) 682
Bme18I GGWCC 1 cut(s) 830
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 830
BmiI GGNNCC 3 cut(s) 686, 778, 898
BmrFI CCNGG 1 cut(s) 682
BmsI GCATC 4 cut(s) 1288, 1693, 1798, 1851
BpiI GAAGAC 3 cut(s) 363, 423, 1736
BpmI CTGGAG 2 cut(s) 195, 664
Bpu10I CCTNAGC 1 cut(s) 1853
Bpu14I TTCGAA 1 cut(s) 1474
BpuEI CTTGAG 2 cut(s) 810, 1797
Bsa29I ATCGAT 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 685
BsaJI CCNNGG 2 cut(s) 539, 573
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 224, 545, 1774
Bse21I CCTNAGG 1 cut(s) 798
Bse3DI GCAATG 1 cut(s) 1034
BseBI CCWGG 1 cut(s) 682
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 2 cut(s) 539, 573
BseGI GGATG 6 cut(s) 778, 820, 1303, 1375, 1783, 1842
BseLI CCNNNNNNNGG 4 cut(s) 132, 224, 545, 1774
BseMI GCAATG 1 cut(s) 1034
BseMII CTCAG 7 cut(s) 224, 516, 789, 969, 1281, 1519, 1844
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 8 cut(s) 346, 349, 400, 973, 1396, 1584, 1587, 1589
BseYI CCCAGC 1 cut(s) 1164
BsgI GTGCAG 1 cut(s) 1596
BshFI GGCC 2 cut(s) 636, 819
BshNI GGYRCC 1 cut(s) 684
BshVI ATCGAT 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 1311
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 4 cut(s) 132, 224, 545, 1774
BsmAI GTCTC 1 cut(s) 1105
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 2 cut(s) 636, 819
BsoBI CYCGRG 1 cut(s) 129
Bsp119I TTCGAA 1 cut(s) 1474
Bsp1286I GDGCHC 1 cut(s) 1311
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 2 cut(s) 386, 871
BspANI GGCC 2 cut(s) 636, 819
BspCNI CTCAG 7 cut(s) 225, 517, 790, 968, 1282, 1518, 1845
BspDI ATCGAT 1 cut(s) 312
BspHI TCATGA 1 cut(s) 1408
BspLI GGNNCC 3 cut(s) 686, 778, 898
BspPI GGATC 2 cut(s) 377, 1083
BspQI GCTCTTC 1 cut(s) 255
BspT104I TTCGAA 1 cut(s) 1474
BspT107I GGYRCC 1 cut(s) 684
BsrDI GCAATG 1 cut(s) 1034
BssECI CCNNGG 2 cut(s) 539, 573
BssNI GRCGYC 1 cut(s) 685
BssT1I CCWWGG 2 cut(s) 539, 573
Bst2UI CCWGG 1 cut(s) 682
Bst4CI ACNGT 1 cut(s) 78
Bst6I CTCTTC 6 cut(s) 255, 803, 1407, 1476, 1551, 1656
BstACI GRCGYC 1 cut(s) 685
BstBI TTCGAA 1 cut(s) 1474
BstC8I GCNNGC 3 cut(s) 8, 12, 1194
BstDEI CTNAG 7 cut(s) 233, 525, 798, 955, 1290, 1505, 1853
BstDSI CCRYGG 1 cut(s) 573
BstENI CCTNNNNNAGG 1 cut(s) 1772
BstF5I GGATG 6 cut(s) 778, 820, 1303, 1375, 1783, 1842
BstH2I RGCGCY 1 cut(s) 688
BstHHI GCGC 1 cut(s) 687
BstMAI GTCTC 1 cut(s) 1105
BstMWI GCNNNNNNNGC 4 cut(s) 569, 684, 983, 1796
BstNI CCWGG 1 cut(s) 682
BstNSI RCATGY 1 cut(s) 10
BstSCI CCNGG 1 cut(s) 680
BstSFI CTRYAG 2 cut(s) 717, 1375
BstV1I GCAGC 8 cut(s) 346, 349, 400, 973, 1396, 1584, 1587, 1589
BstV2I GAAGAC 3 cut(s) 363, 423, 1736
BstX2I RGATCY 5 cut(s) 202, 454, 1075, 1138, 1286
BstYI RGATCY 5 cut(s) 202, 454, 1075, 1138, 1286
Bsu15I ATCGAT 1 cut(s) 312
Bsu36I CCTNAGG 1 cut(s) 798
BsuI GTATCC 2 cut(s) 243, 1678
BsuRI GGCC 2 cut(s) 636, 819
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 573
BtsCI GGATG 6 cut(s) 778, 820, 1303, 1375, 1783, 1842
BtsI GCAGTG 2 cut(s) 1238, 1806
BtsIMutI CAGTG 2 cut(s) 1238, 1806
Cac8I GCNNGC 3 cut(s) 8, 12, 1194
CciI TCATGA 1 cut(s) 1408
CfoI GCGC 1 cut(s) 687
Cfr13I GGNCC 1 cut(s) 830
ClaI ATCGAT 1 cut(s) 312
CspCI CAANNNNNGTGG 4 cut(s) 868, 903, 1214, 1249
CviAII CATG 7 cut(s) 7, 574, 769, 821, 998, 1409, 1604
DdeI CTNAG 7 cut(s) 233, 525, 798, 955, 1290, 1505, 1853
DinI GGCGCC 1 cut(s) 686
EaeI YGGCCR 1 cut(s) 817
Eam1104I CTCTTC 6 cut(s) 255, 803, 1407, 1476, 1551, 1656
EarI CTCTTC 6 cut(s) 255, 803, 1407, 1476, 1551, 1656
Eco130I CCWWGG 2 cut(s) 539, 573
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 830
Eco57I CTGAAG 2 cut(s) 942, 1514
Eco81I CCTNAGG 1 cut(s) 798
Eco88I CYCGRG 1 cut(s) 129
EcoNI CCTNNNNNAGG 1 cut(s) 1772
EcoRII CCWGG 1 cut(s) 680
EcoRV GATATC 1 cut(s) 310
EcoT14I CCWWGG 2 cut(s) 539, 573
EgeI GGCGCC 1 cut(s) 686
EheI GGCGCC 1 cut(s) 686
ErhI CCWWGG 2 cut(s) 539, 573
FaeI CATG 7 cut(s) 10, 577, 772, 824, 1001, 1412, 1607
FalI AAGNNNNNCTT 6 cut(s) 259, 291, 844, 876, 1137, 1169
FatI CATG 7 cut(s) 6, 573, 768, 820, 997, 1408, 1603
FauI CCCGC 1 cut(s) 864
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 9 cut(s) 15, 360, 363, 389, 987, 1385, 1578, 1598, 1601
FokI GGATG 6 cut(s) 785, 827, 1310, 1382, 1790, 1849
Fsp4HI GCNGC 9 cut(s) 15, 360, 363, 389, 987, 1385, 1578, 1598, 1601
FspBI CTAG 3 cut(s) 347, 668, 983
GlaI GCGC 1 cut(s) 686
GluI GCNGC 9 cut(s) 15, 360, 363, 389, 987, 1385, 1578, 1598, 1601
GsaI CCCAGC 1 cut(s) 1168
GsuI CTGGAG 2 cut(s) 195, 664
HaeII RGCGCY 1 cut(s) 688
HaeIII GGCC 2 cut(s) 636, 819
HapII CCGG 1 cut(s) 110
HhaI GCGC 1 cut(s) 687
Hin1I GRCGYC 1 cut(s) 685
Hin1II CATG 7 cut(s) 10, 577, 772, 824, 1001, 1412, 1607
Hin6I GCGC 1 cut(s) 685
HinP1I GCGC 1 cut(s) 685
HincII GTYRAC 2 cut(s) 1320, 1884
HindII GTYRAC 2 cut(s) 1320, 1884
HinfI GANTC 4 cut(s) 117, 608, 1093, 1316
HpaII CCGG 1 cut(s) 110
HphI GGTGA 5 cut(s) 99, 902, 1435, 1686, 1841
Hpy166II GTNNAC 5 cut(s) 101, 406, 1320, 1804, 1884
Hpy8I GTNNAC 5 cut(s) 101, 406, 1320, 1804, 1884
HpyAV CCTTC 5 cut(s) 107, 261, 917, 1248, 1513
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4IV ACGT 1 cut(s) 1724
HpyF10VI GCNNNNNNNGC 4 cut(s) 569, 684, 983, 1796
HpyF3I CTNAG 7 cut(s) 233, 525, 798, 955, 1290, 1505, 1853
HpySE526I ACGT 1 cut(s) 1724
Hsp92I GRCGYC 1 cut(s) 685
Hsp92II CATG 7 cut(s) 10, 577, 772, 824, 1001, 1412, 1607
HspAI GCGC 1 cut(s) 685
KasI GGCGCC 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 592
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 9 cut(s) 220, 430, 577, 683, 727, 902, 931, 1306, 1770
Lsp1109I GCAGC 8 cut(s) 346, 349, 400, 973, 1396, 1584, 1587, 1589
LweI GCATC 4 cut(s) 1288, 1693, 1798, 1851
MaeI CTAG 3 cut(s) 347, 668, 983
MaeII ACGT 1 cut(s) 1724
MaeIII GTNAC 2 cut(s) 438, 484
MfeI CAATTG 2 cut(s) 933, 1280
MflI RGATCY 5 cut(s) 202, 454, 1075, 1138, 1286
MhlI GDGCHC 1 cut(s) 1311
MlsI TGGCCA 1 cut(s) 819
MluNI TGGCCA 1 cut(s) 819
Mly113I GGCGCC 1 cut(s) 685
MlyI GAGTC 1 cut(s) 1325
MmeI TCCRAC 2 cut(s) 227, 1347
Mox20I TGGCCA 1 cut(s) 819
MroXI GAANNNNTTC 3 cut(s) 271, 1185, 1617
MscI TGGCCA 1 cut(s) 819
MseI TTAA 9 cut(s) 60, 150, 180, 627, 642, 1016, 1134, 1202, 1455
MslI CAYNNNNRTG 1 cut(s) 1608
Msp20I TGGCCA 1 cut(s) 819
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 1 cut(s) 682
MunI CAATTG 2 cut(s) 933, 1280
Mva1269I GAATGC 1 cut(s) 188
MvaI CCWGG 1 cut(s) 682
MwoI GCNNNNNNNGC 4 cut(s) 569, 684, 983, 1796
NarI GGCGCC 1 cut(s) 685
NcoI CCATGG 1 cut(s) 573
NlaIII CATG 7 cut(s) 10, 577, 772, 824, 1001, 1412, 1607
NlaIV GGNNCC 3 cut(s) 686, 778, 898
NmuCI GTSAC 1 cut(s) 484
NspI RCATGY 1 cut(s) 10
NspV TTCGAA 1 cut(s) 1474
PaeI GCATGC 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 129
PagI TCATGA 1 cut(s) 1408
PciSI GCTCTTC 1 cut(s) 255
PctI GAATGC 1 cut(s) 188
PdmI GAANNNNTTC 3 cut(s) 271, 1185, 1617
PfeI GAWTC 3 cut(s) 117, 608, 1093
PflFI GACNNNGTC 1 cut(s) 1723
PflMI CCANNNNNTGG 1 cut(s) 545
PkrI GCNGC 9 cut(s) 16, 361, 364, 390, 988, 1386, 1579, 1599, 1602
PleI GAGTC 1 cut(s) 1324
PluTI GGCGCC 1 cut(s) 688
PpsI GAGTC 1 cut(s) 1324
Psp6I CCWGG 1 cut(s) 680
PspFI CCCAGC 1 cut(s) 1164
PspGI CCWGG 1 cut(s) 680
PspN4I GGNNCC 3 cut(s) 686, 778, 898
PspPI GGNCC 1 cut(s) 830
PspXI VCTCGAGB 1 cut(s) 129
PsuI RGATCY 5 cut(s) 202, 454, 1075, 1138, 1286
PsyI GACNNNGTC 1 cut(s) 1723
RseI CAYNNNNRTG 1 cut(s) 1608
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 9 cut(s) 60, 150, 180, 627, 642, 1016, 1134, 1202, 1455
SatI GCNGC 9 cut(s) 15, 360, 363, 389, 987, 1385, 1578, 1598, 1601
Sau96I GGNCC 1 cut(s) 830
SchI GAGTC 1 cut(s) 1325
ScrFI CCNGG 1 cut(s) 682
SduI GDGCHC 1 cut(s) 1311
SfaNI GCATC 4 cut(s) 1288, 1693, 1798, 1851
SfcI CTRYAG 2 cut(s) 717, 1375
SfoI GGCGCC 1 cut(s) 686
Sfr274I CTCGAG 1 cut(s) 129
SfuI TTCGAA 1 cut(s) 1474
SinI GGWCC 1 cut(s) 830
SlaI CTCGAG 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 1608
SmlI CTYRAG 3 cut(s) 129, 825, 1812
SmoI CTYRAG 3 cut(s) 129, 825, 1812
SphI GCATGC 1 cut(s) 10
SsiI CCGC 2 cut(s) 386, 871
SspDI GGCGCC 1 cut(s) 684
SspI AATATT 1 cut(s) 1186
SspMI CTAG 3 cut(s) 347, 668, 983
StyD4I CCNGG 1 cut(s) 680
StyI CCWWGG 2 cut(s) 539, 573
TaaI ACNGT 1 cut(s) 78
TaiI ACGT 1 cut(s) 1727
TaqI TCGA 9 cut(s) 130, 312, 584, 611, 1071, 1474, 1510, 1735, 1782
TfiI GAWTC 3 cut(s) 117, 608, 1093
Tru1I TTAA 9 cut(s) 60, 150, 180, 627, 642, 1016, 1134, 1202, 1455
Tru9I TTAA 9 cut(s) 60, 150, 180, 627, 642, 1016, 1134, 1202, 1455
TscAI CASTG 2 cut(s) 1238, 1806
TseFI GTSAC 1 cut(s) 484
TseI GCWGC 9 cut(s) 14, 359, 362, 388, 986, 1384, 1577, 1597, 1600
Tsp45I GTSAC 1 cut(s) 484
TspDTI ATGAA 8 cut(s) 254, 363, 1221, 1397, 1425, 1532, 1620, 1680
TspGWI ACGGA 1 cut(s) 417
TspRI CASTG 2 cut(s) 1238, 1806
Tth111I GACNNNGTC 1 cut(s) 1723
Van91I CCANNNNNTGG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 830
XagI CCTNNNNNAGG 1 cut(s) 1772
XapI RAATTY 5 cut(s) 650, 690, 1326, 1403, 1477
XbaI TCTAGA 1 cut(s) 346
XceI RCATGY 1 cut(s) 10
XhoI CTCGAG 1 cut(s) 129
XmiI GTMKAC 1 cut(s) 100
XmnI GAANNNNTTC 3 cut(s) 271, 1185, 1617
XspI CTAG 3 cut(s) 347, 668, 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.