Rh2CG358100

PX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008247
Physical Location & Seq
Forward (+)
48454111 .. 48466961
12851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG358100.1

Sequence Viewer

Length: 1815 bp
ATGAGCATGCCTGCTGCCTTTTTATTTATTTGTAACCATAACTTTGTTAGTTTCGCTATTAACATTTACAGAGAACAGTTTTATAGGGTTCAAGTTGGTCTACAATCACCGGAAGGAATCACAACCACTCGAGGGATATTACGAAGATTTAATGATTTTCTAAAGCTATTTTCTGAACTTAAAAAGGCATTCCCCCTGAAAGATCTTCCTCCAGCTCCTCCAAAGGGTTTTCTGAGAATGAAAAGTCGGTTACTATTGGAAGAGCGAAGGTGTTCTTTGGAGGATTGGATAGAAAAGCTGTTATCAGACATCGATATATCAAGAAGTGTGTTGGTTGCAACCTTTCTAGAGCTGGAAGCTGCTGCGAGGTCTTCATTCAATGATCCGCAGCTAATTTCAGATGTAAACTCAACAAGTGGCACAGCTCCGTCTTCTTTGTTACAAACCCAATCAGATCTCTCCGTTGTTGCTGGTAGCTCATCTGTCACATCAGATAATGGTAATGATACTCCATACGAGGTATCTGAGCTTGGAACACCAAGGTATGGAGATGATAGCTTTGCTAATCTTGCCATGGAGCATTCGACATCTGATCAGGACTTAGATGATTCGATAGATACATCTGTTAAGGTTGGACTATTTAACCAGAAATTTGTTCGGGGAAGCCTAGAAAGGCGCTCCAGGCGCCAAATTTCTGGTAGAAGTAATGCGAATACTATAGACAAGGAGCAAACAAGTGAAAATGGTTCTAAACCTCTTTGCATGGATGGAACCGAGTTCTTTGTAGAACCTGAGGAAAGGAAGAGGGATGGCCATGCTCAAGGACCATCAACAGAGAGTTTTGGAAGCGATACTTCTACCTTGCGGGTGGGCAATTTATGTGGTGATGGCTCCCTCAACCTTCCTGAAAGTTCTGAAGCTCCTACAATTGCAGATACTCATGACAACTCAGATTTGAAGTTTTCAAGGGCTATACTAGCTGCTTTTCCACATGATGAGCGACAAAAATTGAACAGAGTTCTGATTACATTGCAACAGAGATTAGGAACAGCAAAAATTGACATCGAGGATCTTATAGCAAGATTGAATCAAGAAGTTGCTGTGAGACAATTTCTCACAACAAAGGTTAAAGATCTGGAAGTGGAACTTGAAACTACCCAGCAAAATTGTAAAGAAAATATTCAGCAGGCTGTCTTAAATGAAAAAGAAAGATTTACCCAAATGCAGTGGGATATGGAGGAACTGCGAAGGAAGTGCCTGGACTTGGAATTACAATTGAGATCTGAGCAGGATGCAAGGGAGCATTCGGAGTTAACAAAAATTTCTATCATTCAGGAGAATGACATATTGATACAGGAGTTGGATGTCTCTAGGCGGCAGCTTGACAACTTGCAGAAATTTCATGAAGAGTTTGAGGTGAAATCAAAGACAGATGTAAAGCTACTTGTTAAAGAGGTCAAATCTCTTCGAAATTCACAATCAGAACTGAAGCAGGAACTCAGTCGATTGATGAAGGAAAAATTAGAAGTAGAGAGAACTCTTCAAAAAGAAAAGCGAAGAATAGAGTGTGCAGCAACTACTAATGCAAAGCTGCTGCATGAATGTGAAATACTTCGCAGTCGGCTTCAAGAATGTAGTGTCAATTTTCTGGTTGAAGAGGAAGATAAACTGATCTTGGATACTTCATCACCATCTGATGCTATTGACCTACTGACAACGTCTGACAATCGAATTGGTCTTCTTCTTGCAGAGGTATGCTTTGCAACTGTAGTTGGTGTCTGTGTCATGTATTTGTGCAATGACTTCTATCAGTAA

Protein Analysis

604

Amino Acids

68.49

Weight (kDa)

5.03

Isoelectric Point (pI)

57.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PX PF00787 44 - 117 6.9e-13 PX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 684
AccB7I CCANNNNNTGG 1 cut(s) 545
AccI GTMKAC 1 cut(s) 100
AciI CCGC 3 cut(s) 386, 865, 1375
AclWI GGATC 2 cut(s) 377, 1077
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 5 cut(s) 650, 690, 1320, 1397, 1471
AcuI CTGAAG 2 cut(s) 936, 1508
AcyI GRCGYC 1 cut(s) 685
AfiI CCNNNNNNNGG 4 cut(s) 132, 224, 545, 1264
AjnI CCWGG 2 cut(s) 680, 1257
Alw26I GTCTC 2 cut(s) 1099, 1372
AlwI GGATC 2 cut(s) 377, 1077
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 1 cut(s) 811
ApeKI GCWGC 9 cut(s) 14, 359, 362, 388, 980, 1378, 1571, 1591, 1594
ApoI RAATTY 5 cut(s) 650, 690, 1320, 1397, 1471
Asp700I GAANNNNTTC 3 cut(s) 271, 1179, 1611
AspLEI GCGC 2 cut(s) 678, 687
AspS9I GGNCC 1 cut(s) 824
AsuHPI GGTGA 4 cut(s) 99, 896, 1429, 1680
AsuII TTCGAA 1 cut(s) 1468
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 1 cut(s) 824
AxyI CCTNAGG 1 cut(s) 792
BaeI ACNNNNGTAYC 4 cut(s) 609, 642, 1343, 1376
BalI TGGCCA 1 cut(s) 813
BanI GGYRCC 1 cut(s) 684
BbsI GAAGAC 3 cut(s) 363, 423, 1730
BbvI GCAGC 8 cut(s) 346, 349, 400, 967, 1390, 1578, 1581, 1583
BccI CCATC 5 cut(s) 761, 803, 835, 881, 1699
BcgI CGANNNNNNTGC 2 cut(s) 1236, 1270
BciT130I CCWGG 2 cut(s) 682, 1259
BciVI GTATCC 1 cut(s) 1672
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 2 cut(s) 1099, 1372
BfaI CTAG 4 cut(s) 347, 668, 977, 1371
BfmI CTRYAG 2 cut(s) 717, 1767
BfoI RGCGCY 2 cut(s) 679, 688
BfuI GTATCC 1 cut(s) 1672
BglII AGATCT 4 cut(s) 202, 454, 1132, 1280
Bme1390I CCNGG 2 cut(s) 682, 1259
Bme18I GGWCC 1 cut(s) 824
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 824
BmiI GGNNCC 3 cut(s) 686, 772, 892
BmrFI CCNGG 2 cut(s) 682, 1259
BmsI GCATC 2 cut(s) 1282, 1687
BpiI GAAGAC 3 cut(s) 363, 423, 1730
BpmI CTGGAG 2 cut(s) 195, 664
Bpu14I TTCGAA 1 cut(s) 1468
BpuEI CTTGAG 1 cut(s) 804
Bsa29I ATCGAT 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 685
BsaJI CCNNGG 2 cut(s) 539, 573
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 224, 545, 1264
Bse21I CCTNAGG 1 cut(s) 792
Bse3DI GCAATG 2 cut(s) 1028, 1804
BseBI CCWGG 2 cut(s) 682, 1259
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 2 cut(s) 539, 573
BseGI GGATG 4 cut(s) 772, 814, 1297, 1369
BseLI CCNNNNNNNGG 4 cut(s) 132, 224, 545, 1264
BseMI GCAATG 2 cut(s) 1028, 1804
BseMII CTCAG 6 cut(s) 224, 516, 783, 963, 1275, 1513
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 8 cut(s) 346, 349, 400, 967, 1390, 1578, 1581, 1583
BseYI CCCAGC 1 cut(s) 1158
BsgI GTGCAG 1 cut(s) 1590
BshFI GGCC 1 cut(s) 813
BshNI GGYRCC 1 cut(s) 684
BshVI ATCGAT 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 4 cut(s) 132, 224, 545, 1264
BsmAI GTCTC 2 cut(s) 1099, 1372
BsmI GAATGC 3 cut(s) 188, 580, 1303
BsnI GGCC 1 cut(s) 813
BsoBI CYCGRG 1 cut(s) 129
Bsp119I TTCGAA 1 cut(s) 1468
Bsp143I GATC 8 cut(s) 202, 382, 454, 592, 1069, 1132, 1280, 1671
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 3 cut(s) 386, 865, 1375
BspANI GGCC 1 cut(s) 813
BspCNI CTCAG 6 cut(s) 225, 517, 784, 962, 1276, 1512
BspDI ATCGAT 1 cut(s) 312
BspHI TCATGA 2 cut(s) 940, 1402
BspLI GGNNCC 3 cut(s) 686, 772, 892
BspPI GGATC 2 cut(s) 377, 1077
BspQI GCTCTTC 1 cut(s) 255
BspT104I TTCGAA 1 cut(s) 1468
BspT107I GGYRCC 1 cut(s) 684
BsrDI GCAATG 2 cut(s) 1028, 1804
BssECI CCNNGG 2 cut(s) 539, 573
BssMI GATC 8 cut(s) 202, 382, 454, 592, 1069, 1132, 1280, 1671
BssNI GRCGYC 1 cut(s) 685
BssT1I CCWWGG 2 cut(s) 539, 573
Bst2UI CCWGG 2 cut(s) 682, 1259
Bst4CI ACNGT 2 cut(s) 78, 1768
Bst6I CTCTTC 6 cut(s) 255, 797, 1401, 1470, 1545, 1650
BstACI GRCGYC 1 cut(s) 685
BstBI TTCGAA 1 cut(s) 1468
BstC8I GCNNGC 3 cut(s) 8, 12, 1188
BstDEI CTNAG 7 cut(s) 233, 525, 601, 792, 949, 1284, 1499
BstDSI CCRYGG 1 cut(s) 573
BstF5I GGATG 4 cut(s) 772, 814, 1297, 1369
BstH2I RGCGCY 2 cut(s) 679, 688
BstHHI GCGC 2 cut(s) 678, 687
BstKTI GATC 8 cut(s) 205, 385, 457, 595, 1072, 1135, 1283, 1674
BstMAI GTCTC 2 cut(s) 1099, 1372
BstMBI GATC 8 cut(s) 202, 382, 454, 592, 1069, 1132, 1280, 1671
BstMWI GCNNNNNNNGC 4 cut(s) 569, 682, 684, 977
BstNI CCWGG 2 cut(s) 682, 1259
BstNSI RCATGY 1 cut(s) 10
BstSCI CCNGG 2 cut(s) 680, 1257
BstSFI CTRYAG 2 cut(s) 717, 1767
BstV1I GCAGC 8 cut(s) 346, 349, 400, 967, 1390, 1578, 1581, 1583
BstV2I GAAGAC 3 cut(s) 363, 423, 1730
BstX2I RGATCY 5 cut(s) 202, 454, 1069, 1132, 1280
BstXI CCANNNNNNTGG 1 cut(s) 695
BstYI RGATCY 5 cut(s) 202, 454, 1069, 1132, 1280
Bsu15I ATCGAT 1 cut(s) 312
Bsu36I CCTNAGG 1 cut(s) 792
BsuI GTATCC 1 cut(s) 1672
BsuRI GGCC 1 cut(s) 813
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 573
BtsCI GGATG 4 cut(s) 772, 814, 1297, 1369
BtsI GCAGTG 1 cut(s) 1232
BtsIMutI CAGTG 1 cut(s) 1232
Cac8I GCNNGC 3 cut(s) 8, 12, 1188
CciI TCATGA 2 cut(s) 940, 1402
CfoI GCGC 2 cut(s) 678, 687
Cfr13I GGNCC 1 cut(s) 824
ClaI ATCGAT 1 cut(s) 312
CspCI CAANNNNNGTGG 4 cut(s) 862, 897, 1208, 1243
CviAII CATG 9 cut(s) 7, 574, 763, 815, 941, 992, 1403, 1598, 1786
DdeI CTNAG 7 cut(s) 233, 525, 601, 792, 949, 1284, 1499
DinI GGCGCC 1 cut(s) 686
DpnI GATC 8 cut(s) 204, 384, 456, 594, 1071, 1134, 1282, 1673
DpnII GATC 8 cut(s) 202, 382, 454, 592, 1069, 1132, 1280, 1671
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 6 cut(s) 255, 797, 1401, 1470, 1545, 1650
EarI CTCTTC 6 cut(s) 255, 797, 1401, 1470, 1545, 1650
Eco130I CCWWGG 2 cut(s) 539, 573
Eco47I GGWCC 1 cut(s) 824
Eco57I CTGAAG 2 cut(s) 936, 1508
Eco81I CCTNAGG 1 cut(s) 792
Eco88I CYCGRG 1 cut(s) 129
EcoRII CCWGG 2 cut(s) 680, 1257
EcoT14I CCWWGG 2 cut(s) 539, 573
EgeI GGCGCC 1 cut(s) 686
EheI GGCGCC 1 cut(s) 686
ErhI CCWWGG 2 cut(s) 539, 573
FaeI CATG 9 cut(s) 10, 577, 766, 818, 944, 995, 1406, 1601, 1789
FalI AAGNNNNNCTT 6 cut(s) 259, 291, 838, 870, 1131, 1163
FatI CATG 9 cut(s) 6, 573, 762, 814, 940, 991, 1402, 1597, 1785
FauI CCCGC 1 cut(s) 858
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 100
FokI GGATG 4 cut(s) 779, 821, 1304, 1376
FspBI CTAG 4 cut(s) 347, 668, 977, 1371
GlaI GCGC 2 cut(s) 677, 686
GsaI CCCAGC 1 cut(s) 1162
GsuI CTGGAG 2 cut(s) 195, 664
HaeII RGCGCY 2 cut(s) 679, 688
HaeIII GGCC 1 cut(s) 813
HapII CCGG 1 cut(s) 110
HhaI GCGC 2 cut(s) 678, 687
Hin1I GRCGYC 1 cut(s) 685
Hin1II CATG 9 cut(s) 10, 577, 766, 818, 944, 995, 1406, 1601, 1789
Hin6I GCGC 2 cut(s) 676, 685
HinP1I GCGC 2 cut(s) 676, 685
HincII GTYRAC 1 cut(s) 1314
HindII GTYRAC 1 cut(s) 1314
HinfI GANTC 3 cut(s) 117, 608, 1087
HpaI GTTAAC 1 cut(s) 1314
HpaII CCGG 1 cut(s) 110
HphI GGTGA 4 cut(s) 99, 896, 1429, 1680
Hpy166II GTNNAC 3 cut(s) 101, 406, 1314
Hpy8I GTNNAC 3 cut(s) 101, 406, 1314
HpyAV CCTTC 5 cut(s) 107, 261, 911, 1242, 1507
HpyCH4III ACNGT 2 cut(s) 78, 1768
HpyCH4IV ACGT 1 cut(s) 1718
HpyF10VI GCNNNNNNNGC 4 cut(s) 569, 682, 684, 977
HpyF3I CTNAG 7 cut(s) 233, 525, 601, 792, 949, 1284, 1499
HpySE526I ACGT 1 cut(s) 1718
Hsp92I GRCGYC 1 cut(s) 685
Hsp92II CATG 9 cut(s) 10, 577, 766, 818, 944, 995, 1406, 1601, 1789
HspAI GCGC 2 cut(s) 676, 685
KasI GGCGCC 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 592
KspAI GTTAAC 1 cut(s) 1314
Kzo9I GATC 8 cut(s) 202, 382, 454, 592, 1069, 1132, 1280, 1671
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 8 cut(s) 220, 430, 577, 683, 727, 896, 925, 1300
Lsp1109I GCAGC 8 cut(s) 346, 349, 400, 967, 1390, 1578, 1581, 1583
LweI GCATC 2 cut(s) 1282, 1687
MaeI CTAG 4 cut(s) 347, 668, 977, 1371
MaeII ACGT 1 cut(s) 1718
MaeIII GTNAC 4 cut(s) 32, 249, 438, 484
MalI GATC 8 cut(s) 204, 384, 456, 594, 1071, 1134, 1282, 1673
MboI GATC 8 cut(s) 202, 382, 454, 592, 1069, 1132, 1280, 1671
MfeI CAATTG 2 cut(s) 927, 1274
MflI RGATCY 5 cut(s) 202, 454, 1069, 1132, 1280
MlsI TGGCCA 1 cut(s) 813
MluNI TGGCCA 1 cut(s) 813
Mly113I GGCGCC 1 cut(s) 685
MmeI TCCRAC 2 cut(s) 613, 1341
Mox20I TGGCCA 1 cut(s) 813
MroXI GAANNNNTTC 3 cut(s) 271, 1179, 1611
MscI TGGCCA 1 cut(s) 813
MseI TTAA 9 cut(s) 60, 150, 180, 627, 642, 1128, 1196, 1313, 1449
MslI CAYNNNNRTG 1 cut(s) 1602
Msp20I TGGCCA 1 cut(s) 813
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 2 cut(s) 682, 1259
MunI CAATTG 2 cut(s) 927, 1274
Mva1269I GAATGC 3 cut(s) 188, 580, 1303
MvaI CCWGG 2 cut(s) 682, 1259
MwoI GCNNNNNNNGC 4 cut(s) 569, 682, 684, 977
NarI GGCGCC 1 cut(s) 685
NcoI CCATGG 1 cut(s) 573
NdeII GATC 8 cut(s) 202, 382, 454, 592, 1069, 1132, 1280, 1671
NlaIII CATG 9 cut(s) 10, 577, 766, 818, 944, 995, 1406, 1601, 1789
NlaIV GGNNCC 3 cut(s) 686, 772, 892
NmuCI GTSAC 1 cut(s) 484
NspI RCATGY 1 cut(s) 10
NspV TTCGAA 1 cut(s) 1468
PaeI GCATGC 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 129
PagI TCATGA 2 cut(s) 940, 1402
PciSI GCTCTTC 1 cut(s) 255
PctI GAATGC 3 cut(s) 188, 580, 1303
PdmI GAANNNNTTC 3 cut(s) 271, 1179, 1611
PfeI GAWTC 3 cut(s) 117, 608, 1087
PflFI GACNNNGTC 1 cut(s) 1717
PflMI CCANNNNNTGG 1 cut(s) 545
PluTI GGCGCC 1 cut(s) 688
Psp6I CCWGG 2 cut(s) 680, 1257
PspFI CCCAGC 1 cut(s) 1158
PspGI CCWGG 2 cut(s) 680, 1257
PspN4I GGNNCC 3 cut(s) 686, 772, 892
PspPI GGNCC 1 cut(s) 824
PspXI VCTCGAGB 1 cut(s) 129
PsuI RGATCY 5 cut(s) 202, 454, 1069, 1132, 1280
PsyI GACNNNGTC 1 cut(s) 1717
RseI CAYNNNNRTG 1 cut(s) 1602
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 9 cut(s) 60, 150, 180, 627, 642, 1128, 1196, 1313, 1449
Sau3AI GATC 8 cut(s) 202, 382, 454, 592, 1069, 1132, 1280, 1671
Sau96I GGNCC 1 cut(s) 824
ScrFI CCNGG 2 cut(s) 682, 1259
SfaNI GCATC 2 cut(s) 1282, 1687
SfcI CTRYAG 2 cut(s) 717, 1767
SfoI GGCGCC 1 cut(s) 686
Sfr274I CTCGAG 1 cut(s) 129
SfuI TTCGAA 1 cut(s) 1468
SinI GGWCC 1 cut(s) 824
SlaI CTCGAG 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 1602
SmlI CTYRAG 2 cut(s) 129, 819
SmoI CTYRAG 2 cut(s) 129, 819
SphI GCATGC 1 cut(s) 10
SsiI CCGC 3 cut(s) 386, 865, 1375
SspDI GGCGCC 1 cut(s) 684
SspI AATATT 1 cut(s) 1180
SspMI CTAG 4 cut(s) 347, 668, 977, 1371
StyD4I CCNGG 2 cut(s) 680, 1257
StyI CCWWGG 2 cut(s) 539, 573
TaaI ACNGT 2 cut(s) 78, 1768
TaiI ACGT 1 cut(s) 1721
TaqI TCGA 8 cut(s) 130, 312, 584, 611, 1065, 1468, 1504, 1729
TauI GCSGC 1 cut(s) 1378
TfiI GAWTC 3 cut(s) 117, 608, 1087
Tru1I TTAA 9 cut(s) 60, 150, 180, 627, 642, 1128, 1196, 1313, 1449
Tru9I TTAA 9 cut(s) 60, 150, 180, 627, 642, 1128, 1196, 1313, 1449
TscAI CASTG 1 cut(s) 1232
TseFI GTSAC 1 cut(s) 484
TseI GCWGC 9 cut(s) 14, 359, 362, 388, 980, 1378, 1571, 1591, 1594
Tsp45I GTSAC 1 cut(s) 484
TspDTI ATGAA 8 cut(s) 254, 363, 1215, 1391, 1419, 1526, 1614, 1674
TspGWI ACGGA 2 cut(s) 417, 451
TspRI CASTG 1 cut(s) 1232
Tth111I GACNNNGTC 1 cut(s) 1717
Van91I CCANNNNNTGG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 5 cut(s) 650, 690, 1320, 1397, 1471
XbaI TCTAGA 1 cut(s) 346
XceI RCATGY 1 cut(s) 10
XhoI CTCGAG 1 cut(s) 129
XmiI GTMKAC 1 cut(s) 100
XmnI GAANNNNTTC 3 cut(s) 271, 1179, 1611
XspI CTAG 4 cut(s) 347, 668, 977, 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.