Rh2BG528800

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0016477
Physical Location & Seq
Reverse (-)
73841837 .. 73843342
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG528800.1

Sequence Viewer

Length: 498 bp
ATGGCCGACTCGGACAACGACTCCGGCGGCCACAACGGCCACTCGCAGGGAGGGGAGCTGTCGGCGCGTGAGCAGGACCGGTTTCTGCCGATCGCGAACGTGAGCCGCATCATGAAGAAGGCATTGCCGGCCAACGCCAAGATCTCCAAGGACGCGAAGGAGACCGTCCAGGAGTGCGTCTCCGAGTTCATCAGCTTCGTCACCGGCGAGGCCTCCGACAAGTGCCAGCGCGAGAAGCGCAAGACGATCAACGGCGACGATTTGCTCTGGGCCATGACCACCTTGGGATTCGAGGAGTACGTCGAGCCGCTCAAGATCTATCTCCAGAAGTACCGCGAGATGGAAGGAGAGAAAGGCGCCGGACACGTGTACGGCTCAGGTGGGTATCAACATCAAATGGGGATAGGGGAGAAGCGCGGTGGTTCCGGCGGTGGAGCTTCAGTGAGGTCAAGGCACGTCACAGAAAGAAGGCACCAGCATCACTATGGCAACAAATAG

Protein Analysis

165

Amino Acids

18.24

Weight (kDa)

7.82

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 28 - 92 9e-28 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14540
fragaria_vesca FvH4_6g40600
malus_domestica MD09G1126900.v1.1
prunus_persica Prupe.3G197300_v2.0.a1
pyrus_communis pycom09g05110
rosa_chinensis RchiOBHm_Chr2g0155961
rosa_laevigata RLG00000020878
rosa_multiflora Rmu_sc0000260.1_g000028
rosa_rugosa Rorug02G0450900
rosa_samantha Rh2AG517500 Rh2BG528800 Rh2CG502200 Rh2DG537900
rosa_wichuraiana Rw2G042610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 356, 471
AccBSI CCGCTC 1 cut(s) 310
AccII CGCG 6 cut(s) 67, 95, 155, 231, 336, 417
AciI CCGC 6 cut(s) 27, 106, 308, 334, 417, 429
AcoI YGGCCR 4 cut(s) 3, 28, 37, 129
AcuI CTGAAG 1 cut(s) 423
AcvI CACGTG 1 cut(s) 367
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 3 cut(s) 299, 332, 371
AfiI CCNNNNNNNGG 2 cut(s) 46, 340
AflIII ACRYGT 2 cut(s) 364, 366
AgeI ACCGGT 1 cut(s) 78
AjiI CACGTC 1 cut(s) 457
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 3 cut(s) 58, 195, 437
AluI AGCT 3 cut(s) 58, 195, 437
Alw26I GTCTC 2 cut(s) 155, 184
AoxI GGCC 6 cut(s) 3, 28, 37, 129, 210, 270
AsiGI ACCGGT 1 cut(s) 78
AspLEI GCGC 5 cut(s) 67, 231, 240, 359, 417
AspS9I GGNCC 2 cut(s) 76, 270
AsuHPI GGTGA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 76
BanI GGYRCC 2 cut(s) 356, 471
BbrPI CACGTG 1 cut(s) 367
BccI CCATC 1 cut(s) 334
BceAI ACGGC 3 cut(s) 52, 268, 388
BciT130I CCWGG 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 155, 184
BfoI RGCGCY 1 cut(s) 360
BglI GCCNNNNNGGC 1 cut(s) 36
BglII AGATCT 2 cut(s) 141, 315
BisI GCNGC 3 cut(s) 28, 106, 308
BlsI GCNGC 3 cut(s) 29, 107, 309
Bme1390I CCNGG 1 cut(s) 170
Bme18I GGWCC 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 457
BmgT120I GGNCC 2 cut(s) 76, 270
BmiI GGNNCC 3 cut(s) 358, 424, 473
BmrFI CCNGG 1 cut(s) 170
BmsI GCATC 2 cut(s) 117, 487
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 1 cut(s) 308
Bpu10I CCTNAGC 1 cut(s) 376
BpuEI CTTGAG 1 cut(s) 296
BsaAI YACGTR 1 cut(s) 367
BsaHI GRCGYC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 147, 282
BsaWI WCCGGW 1 cut(s) 78
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 426, 456
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 340
Bse118I RCCGGY 3 cut(s) 78, 127, 203
Bse3DI GCAATG 1 cut(s) 122
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 2 cut(s) 147, 282
BseLI CCNNNNNNNGG 2 cut(s) 46, 340
BseMI GCAATG 1 cut(s) 122
BseMII CTCAG 1 cut(s) 390
BseRI GAGGAG 1 cut(s) 308
Bsh1236I CGCG 6 cut(s) 67, 95, 155, 231, 336, 417
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 6 cut(s) 5, 30, 39, 131, 212, 272
BshNI GGYRCC 2 cut(s) 356, 471
BshTI ACCGGT 1 cut(s) 78
BsiEI CGRYCG 1 cut(s) 93
BsiSI CCGG 6 cut(s) 24, 79, 128, 204, 360, 426
BslI CCNNNNNNNGG 2 cut(s) 46, 340
BsmAI GTCTC 2 cut(s) 155, 184
BsmBI CGTCTC 1 cut(s) 184
BsnI GGCC 6 cut(s) 5, 30, 39, 131, 212, 272
Bso31I GGTCTC 1 cut(s) 155
Bsp143I GATC 4 cut(s) 90, 141, 246, 315
Bsp68I TCGCGA 1 cut(s) 95
BspACI CCGC 6 cut(s) 27, 106, 308, 334, 417, 429
BspANI GGCC 6 cut(s) 5, 30, 39, 131, 212, 272
BspCNI CTCAG 1 cut(s) 389
BspFNI CGCG 6 cut(s) 67, 95, 155, 231, 336, 417
BspHI TCATGA 1 cut(s) 111
BspLI GGNNCC 3 cut(s) 358, 424, 473
BspT107I GGYRCC 2 cut(s) 356, 471
BspTNI GGTCTC 1 cut(s) 155
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 122
BsrFI RCCGGY 3 cut(s) 78, 127, 203
BssAI RCCGGY 3 cut(s) 78, 127, 203
BssECI CCNNGG 2 cut(s) 147, 282
BssMI GATC 4 cut(s) 90, 141, 246, 315
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 2 cut(s) 147, 282
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 166
BstACI GRCGYC 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 367
BstC8I GCNNGC 2 cut(s) 129, 227
BstDEI CTNAG 1 cut(s) 376
BstFNI CGCG 6 cut(s) 67, 95, 155, 231, 336, 417
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 5 cut(s) 67, 231, 240, 359, 417
BstKTI GATC 4 cut(s) 93, 144, 249, 318
BstMAI GTCTC 2 cut(s) 155, 184
BstMBI GATC 4 cut(s) 90, 141, 246, 315
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 5 cut(s) 36, 64, 128, 235, 237
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 168
BstUI CGCG 6 cut(s) 67, 95, 155, 231, 336, 417
BstX2I RGATCY 2 cut(s) 141, 315
BstYI RGATCY 2 cut(s) 141, 315
BsuRI GGCC 6 cut(s) 5, 30, 39, 131, 212, 272
BtrI CACGTC 1 cut(s) 457
BtsIMutI CAGTG 1 cut(s) 447
BtuMI TCGCGA 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 129, 227
CciI TCATGA 1 cut(s) 111
CfoI GCGC 5 cut(s) 67, 231, 240, 359, 417
Cfr10I RCCGGY 3 cut(s) 78, 127, 203
Cfr13I GGNCC 2 cut(s) 76, 270
CseI GACGC 2 cut(s) 161, 166
Csp6I GTAC 3 cut(s) 298, 331, 370
CspAI ACCGGT 1 cut(s) 78
CviAII CATG 2 cut(s) 112, 274
CviQI GTAC 3 cut(s) 298, 331, 370
DdeI CTNAG 1 cut(s) 376
DinI GGCGCC 1 cut(s) 358
DpnI GATC 4 cut(s) 92, 143, 248, 317
DpnII GATC 4 cut(s) 90, 141, 246, 315
EaeI YGGCCR 4 cut(s) 3, 28, 37, 129
Eco130I CCWWGG 2 cut(s) 147, 282
Eco147I AGGCCT 1 cut(s) 212
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 1 cut(s) 76
Eco57I CTGAAG 1 cut(s) 423
Eco72I CACGTG 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 2 cut(s) 147, 282
EgeI GGCGCC 1 cut(s) 358
EheI GGCGCC 1 cut(s) 358
ErhI CCWWGG 2 cut(s) 147, 282
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 2 cut(s) 115, 277
FaiI YATR 3 cut(s) 113, 275, 486
FatI CATG 2 cut(s) 111, 273
Fnu4HI GCNGC 3 cut(s) 28, 106, 308
Fsp4HI GCNGC 3 cut(s) 28, 106, 308
GlaI GCGC 5 cut(s) 66, 230, 239, 358, 416
GluI GCNGC 3 cut(s) 28, 106, 308
GsuI CTGGAG 1 cut(s) 308
HaeII RGCGCY 1 cut(s) 360
HaeIII GGCC 6 cut(s) 5, 30, 39, 131, 212, 272
HapII CCGG 6 cut(s) 24, 79, 128, 204, 360, 426
HgaI GACGC 2 cut(s) 161, 166
HhaI GCGC 5 cut(s) 67, 231, 240, 359, 417
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 2 cut(s) 115, 277
Hin6I GCGC 5 cut(s) 65, 229, 238, 357, 415
HinP1I GCGC 5 cut(s) 65, 229, 238, 357, 415
HinfI GANTC 3 cut(s) 8, 20, 288
HpaII CCGG 6 cut(s) 24, 79, 128, 204, 360, 426
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 3 cut(s) 13, 184, 217
Hpy188III TCNNGA 4 cut(s) 94, 112, 313, 325
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 2 cut(s) 260, 305
HpyAV CCTTC 4 cut(s) 112, 151, 338, 462
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 4 cut(s) 99, 300, 366, 456
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 64, 128, 235, 237
HpyF3I CTNAG 1 cut(s) 376
HpySE526I ACGT 4 cut(s) 99, 300, 366, 456
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 2 cut(s) 115, 277
HspAI GCGC 5 cut(s) 65, 229, 238, 357, 415
KasI GGCGCC 1 cut(s) 356
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
Kzo9I GATC 4 cut(s) 90, 141, 246, 315
LmnI GCTCC 2 cut(s) 55, 434
LweI GCATC 2 cut(s) 117, 487
MaeII ACGT 4 cut(s) 99, 300, 366, 456
MaeIII GTNAC 2 cut(s) 199, 457
MalI GATC 4 cut(s) 92, 143, 248, 317
MbiI CCGCTC 1 cut(s) 310
MboI GATC 4 cut(s) 90, 141, 246, 315
MboII GAAGA 1 cut(s) 127
MflI RGATCY 2 cut(s) 141, 315
Mly113I GGCGCC 1 cut(s) 357
MlyI GAGTC 2 cut(s) 2, 14
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 5 cut(s) 44, 202, 223, 286, 438
MroNI GCCGGC 1 cut(s) 127
MslI CAYNNNNRTG 1 cut(s) 483
MspI CCGG 6 cut(s) 24, 79, 128, 204, 360, 426
MspR9I CCNGG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 6 cut(s) 67, 95, 155, 231, 336, 417
MwoI GCNNNNNNNGC 5 cut(s) 36, 64, 128, 235, 237
NaeI GCCGGC 1 cut(s) 129
NarI GGCGCC 1 cut(s) 357
NdeII GATC 4 cut(s) 90, 141, 246, 315
NgoMIV GCCGGC 1 cut(s) 127
NlaIII CATG 2 cut(s) 115, 277
NlaIV GGNNCC 3 cut(s) 358, 424, 473
NmuCI GTSAC 2 cut(s) 199, 457
NruI TCGCGA 1 cut(s) 95
PagI TCATGA 1 cut(s) 111
PceI AGGCCT 1 cut(s) 212
PcsI WCGNNNNNNNCGW 2 cut(s) 204, 297
PdiI GCCGGC 1 cut(s) 129
PfeI GAWTC 1 cut(s) 288
PfoI TCCNGGA 1 cut(s) 168
PinAI ACCGGT 1 cut(s) 78
PkrI GCNGC 3 cut(s) 29, 107, 309
Ple19I CGATCG 1 cut(s) 93
PleI GAGTC 2 cut(s) 2, 14
PluTI GGCGCC 1 cut(s) 360
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
PpsI GAGTC 2 cut(s) 2, 14
Ppu21I YACGTR 1 cut(s) 367
Psp6I CCWGG 1 cut(s) 168
PspCI CACGTG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 3 cut(s) 358, 424, 473
PspPI GGNCC 2 cut(s) 76, 270
PsuI RGATCY 2 cut(s) 141, 315
PvuI CGATCG 1 cut(s) 93
RruI TCGCGA 1 cut(s) 95
RsaI GTAC 3 cut(s) 299, 332, 371
RsaNI GTAC 3 cut(s) 298, 331, 370
RseI CAYNNNNRTG 1 cut(s) 483
SatI GCNGC 3 cut(s) 28, 106, 308
Sau3AI GATC 4 cut(s) 90, 141, 246, 315
Sau96I GGNCC 2 cut(s) 76, 270
SchI GAGTC 2 cut(s) 2, 14
ScrFI CCNGG 1 cut(s) 170
SfaNI GCATC 2 cut(s) 117, 487
SfiI GGCCNNNNNGGCC 1 cut(s) 36
SfoI GGCGCC 1 cut(s) 358
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 1 cut(s) 76
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
SseBI AGGCCT 1 cut(s) 212
SsiI CCGC 6 cut(s) 27, 106, 308, 334, 417, 429
SspDI GGCGCC 1 cut(s) 356
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 168
StyI CCWWGG 2 cut(s) 147, 282
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 4 cut(s) 102, 303, 369, 459
TaqI TCGA 2 cut(s) 291, 303
TauI GCSGC 3 cut(s) 30, 108, 310
TfiI GAWTC 1 cut(s) 288
TscAI CASTG 1 cut(s) 447
TseFI GTSAC 2 cut(s) 199, 457
Tsp45I GTSAC 2 cut(s) 199, 457
TspDTI ATGAA 2 cut(s) 128, 178
TspRI CASTG 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 2 cut(s) 280, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.