Rh2BG574200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0020387
Physical Location & Seq
Reverse (-)
79026846 .. 79031652
4807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG574200.1

Sequence Viewer

Length: 345 bp
ATGGTGGTGTTTTGCTTTCTGGTGGATCAGACGAAGCAAGTGCGGGGGAGCAAGCCGGCGGCCGGAATATGCTCGCGGTGCGGCGGTGGAGCTAGCGTCGCTGATATGAAAACCGCCACCAGATTTTGTTACGTGCCGTTGTACTGGAAGTCTTCGAAAGCCATCATATGTACTTTCTGCGGAGCAGTACTTAAATCTGATACATATAGTTCATTGGATCCCCAAGTACCGTTCCTTCCCTCGGTCAAAGTTTCAGTGAAGGCATTACTTGGTATATCATCAGCAGCTCTACCTGCAGCTCCAAGTGCATCAATGCGAATTTTATTCCGTGGACATCATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

12.07

Weight (kDa)

9.61

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020387)

Species Orthologous Gene IDs
malus_domestica MD04G1234000.v1.1 MD08G1086100.v1.1
rosa_multiflora Rmu_sc0000570.1_g000012
rosa_samantha Rh2BG574200 Rh4AG169400
rosa_wichuraiana Rw1G008680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 301
AccII CGCG 1 cut(s) 76
AciI CCGC 7 cut(s) 43, 59, 76, 81, 84, 114, 180
AclWI GGATC 3 cut(s) 33, 212, 225
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 1 cut(s) 318
AfaI GTAC 4 cut(s) 143, 172, 189, 228
AfiI CCNNNNNNNGG 2 cut(s) 62, 241
AluBI AGCT 3 cut(s) 92, 287, 299
AluI AGCT 3 cut(s) 92, 287, 299
AlwI GGATC 3 cut(s) 33, 212, 225
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 2 cut(s) 284, 296
ApoI RAATTY 1 cut(s) 318
AsuII TTCGAA 1 cut(s) 155
AsuNHI GCTAGC 1 cut(s) 92
BamHI GGATCC 1 cut(s) 217
BarI GAAGNNNNNNTAC 2 cut(s) 219, 251
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 2 cut(s) 296, 308
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 121
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 1 cut(s) 294
BfuAI ACCTGC 1 cut(s) 301
BisI GCNGC 4 cut(s) 60, 82, 285, 297
BlsI GCNGC 4 cut(s) 61, 83, 286, 298
BmcAI AGTACT 1 cut(s) 189
BmiI GGNNCC 1 cut(s) 219
BmsI GCATC 1 cut(s) 317
BmtI GCTAGC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 240, 328
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 241
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 149
BseDI CCNNGG 2 cut(s) 240, 328
BseGI GGATG 1 cut(s) 337
BseLI CCNNNNNNNGG 2 cut(s) 62, 241
BseNI ACTGG 1 cut(s) 149
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 2 cut(s) 296, 308
Bsh1236I CGCG 1 cut(s) 76
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 63
BsiSI CCGG 2 cut(s) 56, 63
BslI CCNNNNNNNGG 2 cut(s) 62, 241
BsnI GGCC 1 cut(s) 62
Bsp119I TTCGAA 1 cut(s) 155
Bsp143I GATC 2 cut(s) 25, 217
BspACI CCGC 7 cut(s) 43, 59, 76, 81, 84, 114, 180
BspANI GGCC 1 cut(s) 62
BspFNI CGCG 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 219
BspMAI CTGCAG 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 301
BspOI GCTAGC 1 cut(s) 96
BspPI GGATC 3 cut(s) 33, 212, 225
BspT104I TTCGAA 1 cut(s) 155
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 240, 328
BssMI GATC 2 cut(s) 25, 217
Bst4CI ACNGT 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 133
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 4 cut(s) 53, 57, 74, 94
BstDSI CCRYGG 1 cut(s) 328
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 1 cut(s) 76
BstKTI GATC 2 cut(s) 28, 220
BstMBI GATC 2 cut(s) 25, 217
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 4 cut(s) 78, 98, 293, 305
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 2 cut(s) 296, 308
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BstZI CGGCCG 1 cut(s) 60
BsuRI GGCC 1 cut(s) 62
BtgI CCRYGG 1 cut(s) 328
BtsCI GGATG 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 261
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 4 cut(s) 53, 57, 74, 94
Cfr10I RCCGGY 1 cut(s) 55
CseI GACGC 1 cut(s) 85
Csp6I GTAC 4 cut(s) 142, 171, 188, 227
CviJI RGCY 6 cut(s) 55, 62, 92, 161, 287, 299
CviKI_1 RGCY 6 cut(s) 55, 62, 92, 161, 287, 299
CviQI GTAC 4 cut(s) 142, 171, 188, 227
DpnI GATC 2 cut(s) 27, 219
DpnII GATC 2 cut(s) 25, 217
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
EclXI CGGCCG 1 cut(s) 60
Eco52I CGGCCG 1 cut(s) 60
FaiI YATR 8 cut(s) 70, 107, 167, 169, 205, 207, 275, 343
FauI CCCGC 1 cut(s) 36
FauNDI CATATG 1 cut(s) 167
Fnu4HI GCNGC 4 cut(s) 60, 82, 285, 297
FokI GGATG 1 cut(s) 324
Fsp4HI GCNGC 4 cut(s) 60, 82, 285, 297
FspBI CTAG 1 cut(s) 93
GluI GCNGC 4 cut(s) 60, 82, 285, 297
HaeIII GGCC 1 cut(s) 62
HapII CCGG 2 cut(s) 56, 63
HgaI GACGC 1 cut(s) 85
HpaII CCGG 2 cut(s) 56, 63
Hpy166II GTNNAC 1 cut(s) 332
Hpy188I TCNGA 2 cut(s) 30, 199
Hpy8I GTNNAC 1 cut(s) 332
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 245, 253
HpyCH4III ACNGT 1 cut(s) 231
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 2 cut(s) 296, 308
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 98, 293, 305
HpySE526I ACGT 1 cut(s) 132
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Kzo9I GATC 2 cut(s) 25, 217
LmnI GCTCC 4 cut(s) 48, 89, 182, 304
LpnPI CCDG 6 cut(s) 5, 69, 76, 130, 133, 306
Lsp1109I GCAGC 2 cut(s) 296, 308
LweI GCATC 1 cut(s) 317
MaeI CTAG 1 cut(s) 93
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 128
MalI GATC 2 cut(s) 27, 219
MboI GATC 2 cut(s) 25, 217
MboII GAAGA 1 cut(s) 144
MflI RGATCY 1 cut(s) 217
MluCI AATT 1 cut(s) 318
MnlI CCTC 1 cut(s) 250
MroNI GCCGGC 1 cut(s) 55
MseI TTAA 1 cut(s) 192
MspI CCGG 2 cut(s) 56, 63
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 4 cut(s) 78, 98, 293, 305
NaeI GCCGGC 1 cut(s) 57
NdeI CATATG 1 cut(s) 167
NdeII GATC 2 cut(s) 25, 217
NgoMIV GCCGGC 1 cut(s) 55
NheI GCTAGC 1 cut(s) 92
NlaIV GGNNCC 1 cut(s) 219
NspV TTCGAA 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 57
PkrI GCNGC 4 cut(s) 61, 83, 286, 298
Ppu21I YACGTR 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 219
PstI CTGCAG 1 cut(s) 298
PsuI RGATCY 1 cut(s) 217
RsaI GTAC 4 cut(s) 143, 172, 189, 228
RsaNI GTAC 4 cut(s) 142, 171, 188, 227
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 4 cut(s) 60, 82, 285, 297
Sau3AI GATC 2 cut(s) 25, 217
ScaI AGTACT 1 cut(s) 189
SetI ASST 5 cut(s) 94, 135, 289, 295, 301
SfaNI GCATC 1 cut(s) 317
SfcI CTRYAG 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 155
Sse9I AATT 1 cut(s) 318
SsiI CCGC 7 cut(s) 43, 59, 76, 81, 84, 114, 180
SspMI CTAG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 231
TaiI ACGT 1 cut(s) 135
TaqI TCGA 1 cut(s) 155
TaqII GACCGA 1 cut(s) 232
TasI AATT 1 cut(s) 318
TatI WGTACW 3 cut(s) 141, 170, 187
TauI GCSGC 2 cut(s) 62, 84
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 261
TseI GCWGC 2 cut(s) 284, 296
TspDTI ATGAA 2 cut(s) 122, 201
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 261
XapI RAATTY 1 cut(s) 318
XspI CTAG 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.