Rh2DG012600

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008966
Physical Location & Seq
Reverse (-)
681440 .. 686076
4637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG012600.1

Sequence Viewer

Length: 1176 bp
ATGGCAATGGCGTTAGTACATAGCAACAGTAGCGCTACCATTATGATCGTCTTTTACTCCATATTAGTGTTCATGTTATCTCTCACAACCACCACCATCAATGTGGCTGCGACGGCGAAAGAGCAAGAGCTAGACCGAATCTCAGCGCTGCCGGGACAACCGCCGGTTTCGTTCTCGCAGTATTCTGGTTACGTCACCGTCAACAAGAAACATGGCCGAGCTCTCTTCTACTGGTTCATTGAAGCCACTGCTTCTCCTCAGAACAAACCTCTCGTTCTTTGGCTCAATGGAGGACCAGGTTGTTCATCAGTGGCATATGGAGCATCAGAAGAAATCGGCCCGTTTCGGATTAATACAACCGGTTCATCTCTTTATCTCAACAAGTATTCATGGAACAGAGAAGCGAATATTCTCTTTCTTGAATCGCCTGCTGGAGTCGGTTTCTCATATACAAACACAAGCGCTGACCTTAAAGATTCTGGGGACAAAAGGACTGCTCAGGATGCTCTAACTTTTCTGCTCCAGTGGTTATCAAGATTTCCTCAATACAAATACAGAGAATTCTACATTTCCGGGGAGAGCTATGCAGGGCATTATGTTCCTCAGTTGGCAAAGGAAATTGTCAATTACAATAAAGCACATTCCCATCCCATTATCAATCTAAAGGGGTTTATTGTGGGCAATGCTGTAACAGACAACTATTATGATAGCATTGGAACCGTCGCATATTGGTGGACACATTCCATAATATCTGATCACACCTACAGATCAATCCTCGACCATTGCAATTTCACAGCCGATAAATCAACCACGCAATGCGATGATGCTGTTGATTATGCCATGAACCATGAATTTGGAGACATTGACCAGTACAGTATTTACACACCATTTTGCTTGACACAGATTAATAACGCCAAAAGGCATCTAAGGCTTAAAAACACTCTTCTGCGCCGACGTATCTCTGGGTATGATCCGTGCACTGAGAATTATGCAGAGAAGTACTATAATCGACCAGATGTACAGAAGGCAATGCATGCAAATATTACCAAAATTCCTTACAAGTGGACTGCTTGCAGTGATACTCTTATCAAGAATTGGAATGATTCAGAAGATTCCATGCTGCCTACATACAAGGAGCTGATGGCAGCTGGTCTAAAAATTTGGGTTTTCAGGTAA

Protein Analysis

391

Amino Acids

44.39

Weight (kDa)

7.63

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 51 - 390 1.6e-120 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 161
AclWI GGATC 1 cut(s) 965
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 4 cut(s) 560, 851, 1050, 1158
AfaI GTAC 4 cut(s) 18, 872, 1001, 1020
AfeI AGCGCT 3 cut(s) 34, 147, 463
AgeI ACCGGT 1 cut(s) 359
AgsI TTSAA 2 cut(s) 242, 422
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 5 cut(s) 130, 221, 582, 1138, 1148
AluI AGCT 5 cut(s) 130, 221, 582, 1138, 1148
Alw21I GWGCWC 2 cut(s) 223, 980
Alw26I GTCTC 1 cut(s) 852
Alw44I GTGCAC 1 cut(s) 976
AlwI GGATC 1 cut(s) 965
Aor51HI AGCGCT 3 cut(s) 34, 147, 463
AoxI GGCC 2 cut(s) 214, 337
ApaLI GTGCAC 1 cut(s) 976
ApeKI GCWGC 4 cut(s) 107, 148, 1120, 1145
ApoI RAATTY 4 cut(s) 560, 851, 1050, 1158
AseI ATTAAT 2 cut(s) 351, 906
AsiGI ACCGGT 1 cut(s) 359
AspLEI GCGC 4 cut(s) 35, 148, 464, 951
AspS9I GGNCC 2 cut(s) 293, 338
AsuC2I CCSGG 2 cut(s) 153, 574
AsuHPI GGTGA 1 cut(s) 187
AvaII GGWCC 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 980
BanII GRGCYC 1 cut(s) 223
Bbv12I GWGCWC 2 cut(s) 223, 980
BbvI GCAGC 4 cut(s) 94, 135, 1107, 1157
BccI CCATC 3 cut(s) 104, 654, 1135
BceAI ACGGC 1 cut(s) 129
BciT130I CCWGG 1 cut(s) 297
BclI TGATCA 1 cut(s) 754
BcnI CCSGG 2 cut(s) 153, 574
BcoDI GTCTC 1 cut(s) 852
BfaI CTAG 1 cut(s) 131
BfmI CTRYAG 1 cut(s) 763
BfoI RGCGCY 3 cut(s) 36, 149, 465
BisI GCNGC 4 cut(s) 108, 149, 1121, 1146
BlsI GCNGC 4 cut(s) 109, 150, 1122, 1147
BmcAI AGTACT 1 cut(s) 1001
Bme1390I CCNGG 3 cut(s) 153, 297, 574
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 2 cut(s) 293, 338
BmiI GGNNCC 1 cut(s) 718
BmrFI CCNGG 3 cut(s) 153, 297, 574
BmsI GCATC 4 cut(s) 332, 493, 814, 931
BpmI CTGGAG 2 cut(s) 453, 506
Bpu10I CCTNAGC 1 cut(s) 498
BpuMI CCSGG 2 cut(s) 153, 574
BsaJI CCNNGG 1 cut(s) 573
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bse118I RCCGGY 2 cut(s) 163, 359
Bse1I ACTGG 3 cut(s) 236, 523, 868
Bse3DI GCAATG 5 cut(s) 12, 688, 781, 821, 1035
BseBI CCWGG 1 cut(s) 297
BseDI CCNNGG 1 cut(s) 573
BseGI GGATG 2 cut(s) 508, 646
BseMI GCAATG 5 cut(s) 12, 688, 781, 821, 1035
BseMII CTCAG 5 cut(s) 156, 272, 512, 617, 972
BseNI ACTGG 3 cut(s) 236, 523, 868
BseRI GAGGAG 1 cut(s) 246
BseSI GKGCMC 1 cut(s) 980
BseXI GCAGC 4 cut(s) 94, 135, 1107, 1157
BshFI GGCC 2 cut(s) 216, 339
BshTI ACCGGT 1 cut(s) 359
BsiHKAI GWGCWC 2 cut(s) 223, 980
BsiSI CCGG 4 cut(s) 152, 164, 360, 573
BslFI GGGAC 2 cut(s) 168, 497
BsmAI GTCTC 1 cut(s) 852
BsmFI GGGAC 2 cut(s) 168, 497
BsnI GGCC 2 cut(s) 216, 339
Bsp1286I GDGCHC 2 cut(s) 223, 980
Bsp1407I TGTACA 1 cut(s) 1018
Bsp143I GATC 4 cut(s) 45, 754, 767, 970
BspACI CCGC 1 cut(s) 161
BspANI GGCC 2 cut(s) 216, 339
BspCNI CTCAG 5 cut(s) 155, 271, 511, 616, 973
BspLI GGNNCC 1 cut(s) 718
BspPI GGATC 1 cut(s) 965
BsrDI GCAATG 5 cut(s) 12, 688, 781, 821, 1035
BsrFI RCCGGY 2 cut(s) 163, 359
BsrGI TGTACA 1 cut(s) 1018
BsrI ACTGG 3 cut(s) 236, 523, 868
BssAI RCCGGY 2 cut(s) 163, 359
BssECI CCNNGG 1 cut(s) 573
BssMI GATC 4 cut(s) 45, 754, 767, 970
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 4 cut(s) 29, 199, 721, 875
Bst6I CTCTTC 2 cut(s) 230, 948
BstAPI GCANNNNNTGC 1 cut(s) 1034
BstAUI TGTACA 1 cut(s) 1018
BstC8I GCNNGC 3 cut(s) 429, 1035, 1072
BstDEI CTNAG 6 cut(s) 142, 258, 498, 603, 926, 981
BstF5I GGATG 2 cut(s) 508, 646
BstH2I RGCGCY 3 cut(s) 36, 149, 465
BstHHI GCGC 4 cut(s) 35, 148, 464, 951
BstKTI GATC 4 cut(s) 48, 757, 770, 973
BstMAI GTCTC 1 cut(s) 852
BstMBI GATC 4 cut(s) 45, 754, 767, 970
BstMWI GCNNNNNNNGC 6 cut(s) 30, 113, 320, 503, 928, 1034
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 1 cut(s) 1037
BstSCI CCNGG 3 cut(s) 151, 295, 572
BstSFI CTRYAG 1 cut(s) 763
BstSLI GKGCMC 1 cut(s) 980
BstV1I GCAGC 4 cut(s) 94, 135, 1107, 1157
BstXI CCANNNNNNTGG 2 cut(s) 103, 854
BsuRI GGCC 2 cut(s) 216, 339
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 2 cut(s) 508, 646
BtsI GCAGTG 2 cut(s) 246, 1081
BtsIMutI CAGTG 5 cut(s) 246, 315, 530, 978, 1081
Cac8I GCNNGC 3 cut(s) 429, 1035, 1072
CfoI GCGC 4 cut(s) 35, 148, 464, 951
Cfr10I RCCGGY 2 cut(s) 163, 359
Cfr13I GGNCC 2 cut(s) 293, 338
CsiI ACCWGGT 1 cut(s) 295
Csp6I GTAC 4 cut(s) 17, 871, 1000, 1019
CspAI ACCGGT 1 cut(s) 359
CviAII CATG 7 cut(s) 73, 212, 390, 841, 848, 1034, 1117
CviQI GTAC 4 cut(s) 17, 871, 1000, 1019
DdeI CTNAG 6 cut(s) 142, 258, 498, 603, 926, 981
DpnI GATC 4 cut(s) 47, 756, 769, 972
DpnII GATC 4 cut(s) 45, 754, 767, 970
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 2 cut(s) 230, 948
EarI CTCTTC 2 cut(s) 230, 948
Ecl136II GAGCTC 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 293
Eco47III AGCGCT 3 cut(s) 34, 147, 463
Eco53kI GAGCTC 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 221
EcoRI GAATTC 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 295
EcoT22I ATGCAT 1 cut(s) 1035
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 7 cut(s) 76, 215, 393, 844, 851, 1037, 1120
FaqI GGGAC 2 cut(s) 168, 497
FatI CATG 7 cut(s) 72, 211, 389, 840, 847, 1033, 1116
FauNDI CATATG 1 cut(s) 316
FbaI TGATCA 1 cut(s) 754
Fnu4HI GCNGC 4 cut(s) 108, 149, 1121, 1146
FokI GGATG 2 cut(s) 515, 633
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 4 cut(s) 108, 149, 1121, 1146
FspBI CTAG 1 cut(s) 131
GlaI GCGC 4 cut(s) 34, 147, 463, 950
GluI GCNGC 4 cut(s) 108, 149, 1121, 1146
GsuI CTGGAG 2 cut(s) 453, 506
HaeII RGCGCY 3 cut(s) 36, 149, 465
HaeIII GGCC 2 cut(s) 216, 339
HapII CCGG 4 cut(s) 152, 164, 360, 573
HhaI GCGC 4 cut(s) 35, 148, 464, 951
Hin1II CATG 7 cut(s) 76, 215, 393, 844, 851, 1037, 1120
Hin6I GCGC 4 cut(s) 33, 146, 462, 949
HinP1I GCGC 4 cut(s) 33, 146, 462, 949
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 6 cut(s) 138, 422, 435, 476, 1103, 1112
HpaII CCGG 4 cut(s) 152, 164, 360, 573
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 4 cut(s) 202, 735, 978, 1065
Hpy188I TCNGA 5 cut(s) 261, 328, 348, 754, 1108
Hpy188III TCNNGA 4 cut(s) 419, 500, 534, 1090
Hpy8I GTNNAC 4 cut(s) 202, 735, 978, 1065
Hpy99I CGWCG 3 cut(s) 115, 725, 957
HpyAV CCTTC 1 cut(s) 1018
HpyCH4III ACNGT 4 cut(s) 29, 199, 721, 875
HpyCH4IV ACGT 2 cut(s) 192, 955
HpyCH4V TGCA 7 cut(s) 587, 786, 978, 992, 1033, 1037, 1074
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 113, 320, 503, 928, 1034
HpyF3I CTNAG 6 cut(s) 142, 258, 498, 603, 926, 981
HpySE526I ACGT 2 cut(s) 192, 955
Hsp92II CATG 7 cut(s) 76, 215, 393, 844, 851, 1037, 1120
HspAI GCGC 4 cut(s) 33, 146, 462, 949
Ksp22I TGATCA 1 cut(s) 754
Kzo9I GATC 4 cut(s) 45, 754, 767, 970
LmnI GCTCC 3 cut(s) 320, 525, 1135
Lsp1109I GCAGC 4 cut(s) 94, 135, 1107, 1157
LweI GCATC 4 cut(s) 332, 493, 814, 931
MabI ACCWGGT 1 cut(s) 295
MaeI CTAG 1 cut(s) 131
MaeII ACGT 2 cut(s) 192, 955
MaeIII GTNAC 3 cut(s) 188, 193, 688
MalI GATC 4 cut(s) 47, 756, 769, 972
MboI GATC 4 cut(s) 45, 754, 767, 970
MboII GAAGA 4 cut(s) 217, 341, 935, 1121
MhlI GDGCHC 2 cut(s) 223, 980
MluCI AATT 9 cut(s) 560, 618, 625, 787, 851, 985, 1050, 1093, 1158
MlyI GAGTC 1 cut(s) 444
MnlI CCTC 6 cut(s) 267, 279, 284, 552, 612, 785
Mph1103I ATGCAT 1 cut(s) 1035
MseI TTAA 4 cut(s) 351, 471, 906, 933
MslI CAYNNNNRTG 3 cut(s) 65, 101, 730
MspA1I CMGCKG 1 cut(s) 1148
MspI CCGG 4 cut(s) 152, 164, 360, 573
MspR9I CCNGG 3 cut(s) 153, 297, 574
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 6 cut(s) 30, 113, 320, 503, 928, 1034
NciI CCSGG 2 cut(s) 153, 574
NdeI CATATG 1 cut(s) 316
NdeII GATC 4 cut(s) 45, 754, 767, 970
NlaIII CATG 7 cut(s) 76, 215, 393, 844, 851, 1037, 1120
NlaIV GGNNCC 1 cut(s) 718
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 1 cut(s) 193
NsiI ATGCAT 1 cut(s) 1035
NspI RCATGY 1 cut(s) 1037
PaeI GCATGC 1 cut(s) 1037
PfeI GAWTC 5 cut(s) 138, 422, 476, 1103, 1112
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 4 cut(s) 109, 150, 1122, 1147
PleI GAGTC 1 cut(s) 443
PpsI GAGTC 1 cut(s) 443
PshBI ATTAAT 2 cut(s) 351, 906
Psp124BI GAGCTC 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 718
PspPI GGNCC 2 cut(s) 293, 338
PvuII CAGCTG 1 cut(s) 1148
RsaI GTAC 4 cut(s) 18, 872, 1001, 1020
RsaNI GTAC 4 cut(s) 17, 871, 1000, 1019
RseI CAYNNNNRTG 3 cut(s) 65, 101, 730
SacI GAGCTC 1 cut(s) 223
SaqAI TTAA 4 cut(s) 351, 471, 906, 933
SatI GCNGC 4 cut(s) 108, 149, 1121, 1146
Sau3AI GATC 4 cut(s) 45, 754, 767, 970
Sau96I GGNCC 2 cut(s) 293, 338
ScaI AGTACT 1 cut(s) 1001
SchI GAGTC 1 cut(s) 444
ScrFI CCNGG 3 cut(s) 153, 297, 574
SduI GDGCHC 2 cut(s) 223, 980
SexAI ACCWGGT 1 cut(s) 295
SfaNI GCATC 4 cut(s) 332, 493, 814, 931
SfcI CTRYAG 1 cut(s) 763
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 3 cut(s) 65, 101, 730
SphI GCATGC 1 cut(s) 1037
Sse9I AATT 9 cut(s) 560, 618, 625, 787, 851, 985, 1050, 1093, 1158
SsiI CCGC 1 cut(s) 161
SspI AATATT 2 cut(s) 409, 1042
SspMI CTAG 1 cut(s) 131
SstI GAGCTC 1 cut(s) 223
StyD4I CCNGG 3 cut(s) 151, 295, 572
TaaI ACNGT 4 cut(s) 29, 199, 721, 875
TaiI ACGT 2 cut(s) 195, 958
TaqI TCGA 2 cut(s) 777, 1009
TaqII GACCGA 1 cut(s) 150
TasI AATT 9 cut(s) 560, 618, 625, 787, 851, 985, 1050, 1093, 1158
TatI WGTACW 4 cut(s) 16, 870, 999, 1018
TfiI GAWTC 5 cut(s) 138, 422, 476, 1103, 1112
Tru1I TTAA 4 cut(s) 351, 471, 906, 933
Tru9I TTAA 4 cut(s) 351, 471, 906, 933
TscAI CASTG 5 cut(s) 253, 315, 530, 985, 1081
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 4 cut(s) 107, 148, 1120, 1145
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 7 cut(s) 61, 226, 294, 354, 378, 857, 864
TspGWI ACGGA 1 cut(s) 963
TspRI CASTG 5 cut(s) 253, 315, 530, 985, 1081
VneI GTGCAC 1 cut(s) 976
VpaK11BI GGWCC 1 cut(s) 293
VspI ATTAAT 2 cut(s) 351, 906
XapI RAATTY 4 cut(s) 560, 851, 1050, 1158
XceI RCATGY 1 cut(s) 1037
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XspI CTAG 1 cut(s) 131
ZrmI AGTACT 1 cut(s) 1001
Zsp2I ATGCAT 1 cut(s) 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.