Rh2DG601000

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011361
Physical Location & Seq
Forward (+)
83296641 .. 83300374
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG601000.1

Sequence Viewer

Length: 1431 bp
ATGGATATCGAAGAACTATTGGGCCGAAACCGACCGGCGGCAATCAAGGCGAGGAGTGCGATTTACGTTTGGGGATATAACCAGTCCGGGCAGACTGGTCGGAAGGGCAGAGAGCGCCAGTTGAGGATCCCAAAGCAGCTCTCGCCGGAGCTCTTTGGGTGCCCCGCCGGAGCTAATTCGCGCTGGCTGGACATTGCTTGCGGCCGCGAGCATACCGCAGCTGTAGCTTCAGATGGGTCTCTCTTCACTTGGGGGGCTAATGAATTTGGTCAATTGGGAGATGGAACTGAGGAGAGAAGTAAACATCCAAAGAAAGTGAAGCAATTGGAGACGGAGTTTGTGAAGTCTGTATCTTGTGGAGCACATTGTACAGCTGCCATTGCAGAGCCTCGCAAGAATGATGGAACCATGTCGACAAGTAGGCTTTGGATTTGGGGGCAAAATCAGGGTTCAAATCTGCCGCGTTTATTTTGGGGGGCCTTTGCTCCAAACACGGCTATCCGTCAAGTATCTTGTGGAGCAGCTCATGTGATGGCTTTATCAGAGGATGGCCTGCTACAAGCTTGGGGATACAATGAATTTGGCCAGCTTGGCAGAGGCGTTACATGTGAAGGATTACAAGGGGCTCGTATAATAAATGCTTATGCAAAGTTCCTTGATGAAGCCCCTGAGCTTGTGAAGATTACCCAAGTGTCATGTGGGGAGTACCACACAGCAGCTCTATCTGAAGATGGCGAGGTGTATACTTGGGGGTTGGGAAGCATGGGGCAACTTGGTCATTGTTCTCTTCAATCTGCTGATAAAGAGTTATTGCCAAGGAGAGTGGTCGCACTTGATGCAATCTTCATGAAGGACGTGGCATGTGGCGGTGTGCATACATGTGCTGTGACTCAGAAGGGAGCTCTTTATGCTTGGGGTGGCAGTCGAGCAGGACAATTAGGCCTTGGCCCTGAAACTGGATTCTTTTCATGTAATCCTAATGAGTCTGCAAGCTTTTTCCGAAATATTCCTGCTTTGGTTATTCCACTTGGTGTGCAGCTCATTGCATGTGGACATTCCCATACACTTATTTCTACAAGGGAAGGAAGGATTCATGGATGGGGCTACAATAGCTATGGTCAGGCATCTAATGAGAAATCTACTTATGCTTGGTATCCATCTCCAGTTGATTGGTGTGTTGGGGCAGTTCAAAAACTTGCAGCTGGTGGGGGACATTCAGCTGTGTTGACCGATGCATGTTCCTTGAAGGAATTGTGCGAATTTAGGCTTGCAGATAGTGTGACTCCGACCAATGCTTCTATTATTGAGGACATTGCGTCTAGAACAGATTCAGATAATTTAGTACGCCTATGTGAAAGATTGAGGCAGCACATGCTTGATGGTGGCGACTACGAATTTGAAGATGATGAGCCAAATAGTTATAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
RCC1

Protein Analysis

476

Amino Acids

51.62

Weight (kDa)

6.09

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 11 - 152 3.1e-27 RCC1-like domain
RCC1 PF00415 21 - 75 1.2e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 64 - 91 4.3e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 78 - 127 1.1e-15 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 162 - 408 5.1e-54 RCC1-like domain
RCC1_2 PF13540 167 - 196 6.7e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 183 - 241 1.1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 228 - 257 1.6e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 244 - 296 5.3e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 284 - 312 1.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 299 - 356 6.6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 346 - 374 3.7e-09 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011361)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02300 AT3G02300
fragaria_vesca FvH4_6g46400 FvH4_6g46400
malus_domestica MD09G1074200.v1.1 MD17G1065600.v1.1
prunus_persica Prupe.3G249200_v2.0.a1 Prupe.3G249200_v2.0.a1
pyrus_communis pycom09g00130 pycom17g06550
rosa_chinensis RchiOBHm_Chr2g0164931
rosa_laevigata RLG00000021498
rosa_multiflora Rmu_sc0001097.1_g000002
rosa_roxburghii Rroxscaffold_2G00086200
rosa_rugosa Rorug02G0511300
rosa_samantha Rh2AG579600 Rh2BG590600 Rh2CG561300 Rh2DG601000
rosa_wichuraiana Rw2G048110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 159
AccI GTMKAC 2 cut(s) 413, 743
AccII CGCG 3 cut(s) 181, 207, 463
AciI CCGC 7 cut(s) 38, 165, 201, 205, 216, 461, 867
AclWI GGATC 2 cut(s) 121, 134
AcoI YGGCCR 2 cut(s) 202, 583
AcsI RAATTY 4 cut(s) 263, 578, 1259, 1394
AcuI CTGAAG 2 cut(s) 213, 747
AdeI CACNNNGTG 1 cut(s) 1031
AfaI GTAC 3 cut(s) 370, 707, 1344
AfiI CCNNNNNNNGG 2 cut(s) 37, 956
AflIII ACRYGT 2 cut(s) 605, 878
AgsI TTSAA 5 cut(s) 453, 791, 1190, 1246, 1400
AhdI GACNNNNNGTC 1 cut(s) 1315
AjiI CACGTC 1 cut(s) 856
Alw21I GWGCWC 3 cut(s) 153, 364, 904
Alw26I GTCTC 2 cut(s) 243, 323
AlwI GGATC 2 cut(s) 121, 134
AoxI GGCC 7 cut(s) 22, 202, 477, 550, 583, 940, 946
ApeKI GCWGC 8 cut(s) 136, 218, 374, 521, 716, 1036, 1199, 1366
ApoI RAATTY 4 cut(s) 263, 578, 1259, 1394
ArsI GACNNNNNNTTYG 2 cut(s) 1379, 1411
Asp700I GAANNNNTTC 1 cut(s) 1327
AspLEI GCGC 2 cut(s) 117, 183
AspS9I GGNCC 3 cut(s) 22, 477, 947
AsuC2I CCSGG 1 cut(s) 88
BaeGI GKGCMC 1 cut(s) 164
BalI TGGCCA 1 cut(s) 585
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 159
BanII GRGCYC 3 cut(s) 153, 628, 904
Bbv12I GWGCWC 3 cut(s) 153, 364, 904
BbvI GCAGC 8 cut(s) 148, 230, 361, 533, 728, 1048, 1211, 1378
BccI CCATC 9 cut(s) 227, 275, 395, 526, 542, 725, 1092, 1165, 1373
BceAI ACGGC 1 cut(s) 510
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciVI GTATCC 2 cut(s) 563, 1164
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 2 cut(s) 243, 323
BfaI CTAG 1 cut(s) 1320
BfmI CTRYAG 1 cut(s) 222
BfoI RGCGCY 1 cut(s) 118
BfuI GTATCC 2 cut(s) 563, 1164
BglI GCCNNNNNGGC 1 cut(s) 591
Bme1390I CCNGG 1 cut(s) 88
BmeRI GACNNNNNGTC 1 cut(s) 1315
BmgBI CACGTC 1 cut(s) 856
BmgT120I GGNCC 3 cut(s) 22, 477, 947
BmiI GGNNCC 4 cut(s) 128, 161, 406, 478
BmrFI CCNGG 1 cut(s) 88
BmsI GCATC 3 cut(s) 826, 1133, 1222
BpmI CTGGAG 1 cut(s) 1146
Bpu10I CCTNAGC 1 cut(s) 669
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 2 cut(s) 815, 943
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 956
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 5 cut(s) 82, 100, 118, 961, 1163
Bse3DI GCAATG 4 cut(s) 192, 378, 1041, 1311
BseDI CCNNGG 2 cut(s) 815, 943
BseGI GGATG 3 cut(s) 304, 553, 1103
BseLI CCNNNNNNNGG 2 cut(s) 37, 956
BseMI GCAATG 4 cut(s) 192, 378, 1041, 1311
BseMII CTCAG 3 cut(s) 279, 660, 905
BseNI ACTGG 5 cut(s) 82, 100, 118, 961, 1163
BseRI GAGGAG 2 cut(s) 67, 305
BseSI GKGCMC 1 cut(s) 164
BseX3I CGGCCG 1 cut(s) 202
BseXI GCAGC 8 cut(s) 148, 230, 361, 533, 728, 1048, 1211, 1378
BsgI GTGCAG 1 cut(s) 1055
Bsh1236I CGCG 3 cut(s) 181, 207, 463
Bsh1285I CGRYCG 2 cut(s) 35, 205
BshFI GGCC 7 cut(s) 24, 204, 479, 552, 585, 942, 948
BshNI GGYRCC 1 cut(s) 159
BsiEI CGRYCG 2 cut(s) 35, 205
BsiHKAI GWGCWC 3 cut(s) 153, 364, 904
BsiSI CCGG 4 cut(s) 35, 87, 146, 168
BslFI GGGAC 1 cut(s) 1224
BslI CCNNNNNNNGG 2 cut(s) 37, 956
BsmAI GTCTC 2 cut(s) 243, 323
BsmBI CGTCTC 1 cut(s) 323
BsmFI GGGAC 1 cut(s) 1224
BsnI GGCC 7 cut(s) 24, 204, 479, 552, 585, 942, 948
Bso31I GGTCTC 1 cut(s) 243
Bsp1286I GDGCHC 5 cut(s) 153, 164, 364, 628, 904
Bsp1407I TGTACA 1 cut(s) 368
Bsp143I GATC 1 cut(s) 126
BspACI CCGC 7 cut(s) 38, 165, 201, 205, 216, 461, 867
BspANI GGCC 7 cut(s) 24, 204, 479, 552, 585, 942, 948
BspCNI CTCAG 3 cut(s) 280, 661, 904
BspFNI CGCG 3 cut(s) 181, 207, 463
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 4 cut(s) 128, 161, 406, 478
BspPI GGATC 2 cut(s) 121, 134
BspT107I GGYRCC 1 cut(s) 159
BspTNI GGTCTC 1 cut(s) 243
BsrDI GCAATG 4 cut(s) 192, 378, 1041, 1311
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 368
BsrI ACTGG 5 cut(s) 82, 100, 118, 961, 1163
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 815, 943
BssMI GATC 1 cut(s) 126
BssNAI GTATAC 1 cut(s) 744
BssT1I CCWWGG 2 cut(s) 815, 943
Bst1107I GTATAC 1 cut(s) 744
Bst6I CTCTTC 2 cut(s) 248, 792
BstAPI GCANNNNNTGC 2 cut(s) 836, 1372
BstAUI TGTACA 1 cut(s) 368
BstC8I GCNNGC 7 cut(s) 185, 199, 209, 554, 587, 991, 1269
BstDEI CTNAG 3 cut(s) 288, 669, 891
BstF5I GGATG 3 cut(s) 304, 553, 1103
BstFNI CGCG 3 cut(s) 181, 207, 463
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 117, 183
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 2 cut(s) 243, 323
BstMBI GATC 1 cut(s) 126
BstMCI CGRYCG 2 cut(s) 35, 205
BstNSI RCATGY 6 cut(s) 609, 864, 882, 1050, 1239, 1375
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 1 cut(s) 222
BstSLI GKGCMC 1 cut(s) 164
BstUI CGCG 3 cut(s) 181, 207, 463
BstV1I GCAGC 8 cut(s) 148, 230, 361, 533, 728, 1048, 1211, 1378
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 1170
BstYI RGATCY 1 cut(s) 126
BstZ17I GTATAC 1 cut(s) 744
BstZI CGGCCG 1 cut(s) 202
BsuI GTATCC 2 cut(s) 563, 1164
BsuRI GGCC 7 cut(s) 24, 204, 479, 552, 585, 942, 948
BtrI CACGTC 1 cut(s) 856
BtsCI GGATG 3 cut(s) 304, 553, 1103
Cac8I GCNNGC 7 cut(s) 185, 199, 209, 554, 587, 991, 1269
CciI TCATGA 1 cut(s) 846
CciNI GCGGCCGC 1 cut(s) 202
CfoI GCGC 2 cut(s) 117, 183
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 22, 477, 947
CseI GACGC 1 cut(s) 1305
Csp6I GTAC 3 cut(s) 369, 706, 1343
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
CviQI GTAC 3 cut(s) 369, 706, 1343
DdeI CTNAG 3 cut(s) 288, 669, 891
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
DraIII CACNNNGTG 1 cut(s) 1031
DriI GACNNNNNGTC 1 cut(s) 1315
EaeI YGGCCR 2 cut(s) 202, 583
EagI CGGCCG 1 cut(s) 202
Eam1104I CTCTTC 2 cut(s) 248, 792
Eam1105I GACNNNNNGTC 1 cut(s) 1315
EarI CTCTTC 2 cut(s) 248, 792
Ecl136II GAGCTC 2 cut(s) 151, 902
EclXI CGGCCG 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 815, 943
Eco147I AGGCCT 1 cut(s) 942
Eco24I GRGCYC 3 cut(s) 153, 628, 904
Eco31I GGTCTC 1 cut(s) 243
Eco32I GATATC 1 cut(s) 7
Eco52I CGGCCG 1 cut(s) 202
Eco53kI GAGCTC 2 cut(s) 151, 902
Eco57I CTGAAG 2 cut(s) 213, 747
EcoICRI GAGCTC 2 cut(s) 151, 902
EcoO109I RGGNCCY 1 cut(s) 477
EcoRV GATATC 1 cut(s) 7
EcoT14I CCWWGG 2 cut(s) 815, 943
EcoT22I ATGCAT 1 cut(s) 1237
EcoT38I GRGCYC 3 cut(s) 153, 628, 904
ErhI CCWWGG 2 cut(s) 815, 943
Esp3I CGTCTC 1 cut(s) 323
FaqI GGGAC 1 cut(s) 1224
FauI CCCGC 1 cut(s) 172
FblI GTMKAC 2 cut(s) 413, 743
FokI GGATG 3 cut(s) 291, 560, 1110
FriOI GRGCYC 3 cut(s) 153, 628, 904
FspBI CTAG 1 cut(s) 1320
GlaI GCGC 2 cut(s) 116, 182
GsuI CTGGAG 1 cut(s) 1146
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 7 cut(s) 24, 204, 479, 552, 585, 942, 948
HapII CCGG 4 cut(s) 35, 87, 146, 168
HgaI GACGC 1 cut(s) 1305
HhaI GCGC 2 cut(s) 117, 183
Hin6I GCGC 2 cut(s) 115, 181
HinP1I GCGC 2 cut(s) 115, 181
HincII GTYRAC 2 cut(s) 414, 1227
HindII GTYRAC 2 cut(s) 414, 1227
HindIII AAGCTT 2 cut(s) 561, 991
HinfI GANTC 6 cut(s) 889, 960, 983, 1090, 1282, 1328
HpaII CCGG 4 cut(s) 35, 87, 146, 168
Hpy166II GTNNAC 5 cut(s) 302, 414, 744, 1052, 1227
Hpy188I TCNGA 8 cut(s) 102, 232, 544, 727, 894, 1001, 1287, 1333
Hpy188III TCNNGA 2 cut(s) 847, 1320
Hpy8I GTNNAC 5 cut(s) 302, 414, 744, 1052, 1227
HpyAV CCTTC 7 cut(s) 97, 605, 844, 889, 1076, 1080, 1240
HpyCH4IV ACGT 2 cut(s) 66, 855
HpyF3I CTNAG 3 cut(s) 288, 669, 891
HpySE526I ACGT 2 cut(s) 66, 855
HspAI GCGC 2 cut(s) 115, 181
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 6 cut(s) 148, 170, 359, 490, 518, 899
Lsp1109I GCAGC 8 cut(s) 148, 230, 361, 533, 728, 1048, 1211, 1378
LweI GCATC 3 cut(s) 826, 1133, 1222
MaeI CTAG 1 cut(s) 1320
MaeII ACGT 2 cut(s) 66, 855
MaeIII GTNAC 3 cut(s) 601, 886, 1279
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MboII GAAGA 7 cut(s) 23, 235, 691, 740, 779, 835, 1412
MfeI CAATTG 2 cut(s) 272, 323
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 5 cut(s) 153, 164, 364, 628, 904
MlsI TGGCCA 1 cut(s) 585
MluNI TGGCCA 1 cut(s) 585
MlyI GAGTC 3 cut(s) 883, 992, 1276
MmeI TCCRAC 2 cut(s) 80, 1310
MnlI CCTC 9 cut(s) 45, 117, 283, 399, 538, 590, 730, 1300, 1356
Mox20I TGGCCA 1 cut(s) 585
Mph1103I ATGCAT 1 cut(s) 1237
MroXI GAANNNNTTC 1 cut(s) 1327
MscI TGGCCA 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 879
Msp20I TGGCCA 1 cut(s) 585
MspA1I CMGCKG 4 cut(s) 221, 374, 1202, 1220
MspI CCGG 4 cut(s) 35, 87, 146, 168
MspR9I CCNGG 1 cut(s) 88
MunI CAATTG 2 cut(s) 272, 323
MvnI CGCG 3 cut(s) 181, 207, 463
NciI CCSGG 1 cut(s) 88
NdeII GATC 1 cut(s) 126
NlaIV GGNNCC 4 cut(s) 128, 161, 406, 478
NmuCI GTSAC 2 cut(s) 886, 1279
NotI GCGGCCGC 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 1237
NspI RCATGY 6 cut(s) 609, 864, 882, 1050, 1239, 1375
PagI TCATGA 1 cut(s) 846
PceI AGGCCT 1 cut(s) 942
PciI ACATGT 2 cut(s) 605, 878
PdmI GAANNNNTTC 1 cut(s) 1327
PfeI GAWTC 3 cut(s) 960, 1090, 1328
PleI GAGTC 3 cut(s) 883, 991, 1276
PpsI GAGTC 3 cut(s) 883, 991, 1276
PscI ACATGT 2 cut(s) 605, 878
Psp124BI GAGCTC 2 cut(s) 153, 904
PspN4I GGNNCC 4 cut(s) 128, 161, 406, 478
PspPI GGNCC 3 cut(s) 22, 477, 947
PsuI RGATCY 1 cut(s) 126
PvuII CAGCTG 4 cut(s) 221, 374, 1202, 1220
RsaI GTAC 3 cut(s) 370, 707, 1344
RsaNI GTAC 3 cut(s) 369, 706, 1343
RseI CAYNNNNRTG 1 cut(s) 879
SacI GAGCTC 2 cut(s) 153, 904
SalI GTCGAC 1 cut(s) 412
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 3 cut(s) 22, 477, 947
SchI GAGTC 3 cut(s) 883, 992, 1276
ScrFI CCNGG 1 cut(s) 88
SduI GDGCHC 5 cut(s) 153, 164, 364, 628, 904
SfaNI GCATC 3 cut(s) 826, 1133, 1222
SfcI CTRYAG 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 879
SseBI AGGCCT 1 cut(s) 942
SsiI CCGC 7 cut(s) 38, 165, 201, 205, 216, 461, 867
SspI AATATT 1 cut(s) 1006
SspMI CTAG 1 cut(s) 1320
SstI GAGCTC 2 cut(s) 153, 904
StuI AGGCCT 1 cut(s) 942
StyD4I CCNGG 1 cut(s) 86
StyI CCWWGG 2 cut(s) 815, 943
TaiI ACGT 2 cut(s) 69, 858
TaqI TCGA 3 cut(s) 9, 413, 925
TaqII GACCGA 1 cut(s) 1244
TatI WGTACW 1 cut(s) 368
TauI GCSGC 4 cut(s) 41, 204, 207, 463
TfiI GAWTC 3 cut(s) 960, 1090, 1328
TseFI GTSAC 2 cut(s) 886, 1279
TseI GCWGC 8 cut(s) 136, 218, 374, 521, 716, 1036, 1199, 1366
Tsp45I GTSAC 2 cut(s) 886, 1279
TspDTI ATGAA 7 cut(s) 276, 591, 675, 835, 863, 957, 1082
TspGWI ACGGA 2 cut(s) 347, 491
XapI RAATTY 4 cut(s) 263, 578, 1259, 1394
XbaI TCTAGA 1 cut(s) 1319
XceI RCATGY 6 cut(s) 609, 864, 882, 1050, 1239, 1375
XcmI CCANNNNNNNNNTGG 1 cut(s) 695
XmiI GTMKAC 2 cut(s) 413, 743
XmnI GAANNNNTTC 1 cut(s) 1327
XspI CTAG 1 cut(s) 1320
Zsp2I ATGCAT 1 cut(s) 1237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.