Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0003433

Physical Location & Seq

Genomic Coordinates Forward (+)

38660528 .. 38670412

Gene Structure 9885 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG153000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGAGTGATTCCATTTTATTTTTTTACCGTTTTTTGTACCACAATGCTTGTATTACAGTGAGAAATTATTTTATGATTGAACAGGTGAGGTACAAGAATTGGCTTTGGTGGACTCGATTTGCAACTGTTATTACAGTACTGCAATTTGTTGGTGCATCTTCCCTGCTGTTCAGTATGGTCAACTACATGATCTACTCTCAAAACAAAGCATCAAGTTATTGTGTTCTGGTGGCAACTTCAAGCAATATTTTGTGGAAGCAACAACTACTCATCTTTTTTGTGATCACGGTGTGTTTGGCTGCGCTGCTACAATGTTTGGCTGGATCTAATGTTTTAAAATGGAGATCCTTTTATGCAACCCAAGATGATGCATGGAAAGCTCATTACAGGGAGGTATTTGATCATGGTATTCGCGAGGCCTTGTGCTGTATGGGACGAGTCAAATACTTGAGTGTATTGGAAGAAGATGAGGTTTTCTCAGTAGCTCGACTGCTGGGTGACCTTGTTGCCTATCGTGCAGCAGGCACAGGACATCTGAAACTTATGGCAGGTCTCGCACTATTGCGGAACCAGAGCCATTCACCAAAATCCTTTGACGAGTGCATGGAGACACCTGAAGAAAAGATCAGGGAGGCTGCAGATCTTCATAAGTTTGCAGAAGCTGCCTACACGGGTCCATTACTTGATTTTGGAAGACACCCTTTCTTATTTCCTTGTGCTTGGCTTTATAGGCAAGGGATTTTGACCCCATGGACTCGTAACAGGCTACCCAAACTTGATGGTGATAATTGGTGGCGAGGTCATGCAGCGGCGTTTCTAAAATATGTAAAGTTATCCCCAGAGGTACTTAGACAAGGGCGTGTTAACCAGGCAAAGTGTAGAGCTGCATACTTTGTTATAGTGCTGCATCATCTAAGATCCGTGGTGATTGCTGTTCGGGGAACTGAGACCCCAGAAGATCTAATTACTGACAGCTTATGTAGGGAATGTCGTCTTTCTGCAGAAGACTTGGATGGATTGATAAATAGCTGTCTTCTTCAACGCGATGTAAAAGAGAGCTTGATGTCTTTCCCTCACCACGCACACTCTGGTATAGTTGAGGCTGCAAGAGATCTTTTTATGCAAATTGAAGGCTCTGACTCAAGTGGCCTTCTTTCTTCTTTATTGGGAGCTGGATGTGAGTGTGAGGGATATAGCATTCGCATAGTGGGGCATTCCCTAGGAGGTGCGATTGGTGCATTGCTAGGACTCAGACTGTATCACCGATACCCAAATTTGCATGTCTATACATATGGCCCCCTTCCATGTGTGGACTCAGTTGTAGCAAATGCATGTTCTGAATTTATTACAAGCATTGTATACAACAATGAATTTTCAGCACGCCTTTCTGTTGGATCTCTCCTGCGGCTTCAAGGTGCTGCAATTATGGCAATGTCAAAAGATACAGAAGCCGATTCAGCTATGATATTAAGGCTTGCACGTCATTTTCTTAATGTAAGCAAACATCAGAAAGATAGGACTCAGGTAAAGGATCCAGCTTCAGATGCCATTTCTGGGGCAATCACTGCCGAGAAGCTTAGCCACCAAATCTGCGGACCTCAGTTTGATATAAAAGCAAGTGAGGAACAAGATGAGGGTTTCCTTGCGTGCAAGGATGCTCGCACAGGTGAAACAGATTTTGGAGCTGATAACGATCAATTTACTAACCCTTTCGCCAGTGATGCCATTGCAAATCATGATCCTGTGTCTGAGTTTATGGAAAGTGTCCCCAGATCTGATAGTGTGTCACCTACCGATCCCCCTGAGATGTATCTGCCAGGCCTTGTAATTCATATAGTAGCACAGCCGAGGAACTTCGATATGCCTATTTGGAAAGGCTGGGGAGTCCACGAGAAGACAAAATCTCACAAAGCTTATATTGCAGACAGAGAAAGTTTCAAAGATATGATTGTGTCACCATCAATGTTTCTCGACCATCTTCCTTGGAGATGTCACGATGCAATGCTAAAATTATTGCAAGCTCGGAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0001676 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006690 GO:0007275 GO:0007399 GO:0007405 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009987 GO:0016020 GO:0019369 GO:0019752 GO:0022008 GO:0030154 GO:0032501 GO:0032502 GO:0032787 GO:0033559 GO:0042133 GO:0042136 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044424 GO:0044464 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0061351 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0072089 GO:1901568

KEGG Pathways

Metabolic & Signaling

ko04723 ko04745 ko04925 map04723 map04745 map04925

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

676

Amino Acids

76.03

Weight (kDa)

6.49

Isoelectric Point (pI)

44.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7358	PF24057	27 - 171	4e-68	Domain of unknown function (DUF7358)
Lipase_3	PF01764	309 - 458	2.7e-12	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003433)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05790 AT1G05790 AT1G05790 AT1G05790
fragaria_vesca	FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690
malus_domestica	MD04G1032500.v1.1 MD04G1032600.v1.1
prunus_persica	Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1
pyrus_communis	pycom04g02680 pycom04g02690
rosa_chinensis	RchiOBHm_Chr4g0408981
rosa_laevigata	RLG00000008601
rosa_multiflora	Rmu_sc0003685.1_g000003 Rmu_sc0031886.1_g000002
rosa_roxburghii	Rroxscaffold_5G00353410
rosa_rugosa	Rorug04G0088900 Rorug04G0088900
rosa_samantha	Rh4AG153000 Rh4BG150900 Rh4CG161400 Rh4DG146400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	539
AccI	GTMKAC	1 cut(s)	1359
AccII	CGCG	2 cut(s)	414, 1044
AciI	CCGC	4 cut(s)	565, 809, 1405, 1593
AclWI	GGATC	8 cut(s)	331, 339, 912, 1402, 1526, 1539, 1733, 1790
AcsI	RAATTY	3 cut(s)	1273, 1340, 1370
AcuI	CTGAAG	2 cut(s)	636, 1524
AdeI	CACNNNGTG	1 cut(s)	291
AfaI	GTAC	4 cut(s)	38, 92, 138, 846
AfiI	CCNNNNNNNGG	1 cut(s)	1554
AgsI	TTSAA	6 cut(s)	80, 240, 1040, 1130, 1412, 1939
AjiI	CACGTC	1 cut(s)	1481
AjnI	CCWGG	2 cut(s)	867, 1816
AjuI	GAANNNNNNNTTGG	2 cut(s)	1662, 1694
Alw26I	GTCTC	3 cut(s)	557, 602, 941
AlwI	GGATC	8 cut(s)	331, 339, 912, 1402, 1526, 1539, 1733, 1790
AlwNI	CAGNNNCTG	1 cut(s)	662
AoxI	GGCC	4 cut(s)	417, 1147, 1294, 1819
ApoI	RAATTY	3 cut(s)	1273, 1340, 1370
AspA2I	CCTAGG	1 cut(s)	1219
AspLEI	GCGC	1 cut(s)	304
AspS9I	GGNCC	3 cut(s)	674, 1295, 1595
AvaII	GGWCC	2 cut(s)	674, 1595
AvrII	CCTAGG	1 cut(s)	1219
BamHI	GGATCC	1 cut(s)	1531
BauI	CACGAG	1 cut(s)	1889
BbsI	GAAGAC	4 cut(s)	700, 1011, 1025, 1901
BccI	CCATC	4 cut(s)	773, 1007, 1966, 1983
BciT130I	CCWGG	2 cut(s)	869, 1818
BclI	TGATCA	2 cut(s)	282, 400
BcoDI	GTCTC	3 cut(s)	557, 602, 941
BfaI	CTAG	3 cut(s)	1220, 1244, 2029
BfmI	CTRYAG	2 cut(s)	636, 999
BfuAI	ACCTGC	1 cut(s)	539
BglII	AGATCT	4 cut(s)	640, 958, 1111, 1772
BlnI	CCTAGG	1 cut(s)	1219
BlpI	GCTNAGC	1 cut(s)	1577
BmcAI	AGTACT	1 cut(s)	138
Bme1390I	CCNGG	2 cut(s)	869, 1818
Bme18I	GGWCC	2 cut(s)	674, 1595
BmgBI	CACGTC	1 cut(s)	1481
BmgT120I	GGNCC	3 cut(s)	674, 1295, 1595
BmiI	GGNNCC	4 cut(s)	569, 675, 1297, 1533
BmrFI	CCNGG	2 cut(s)	869, 1818
BmsI	GCATC	8 cut(s)	164, 218, 358, 916, 1534, 1645, 1711, 1987
BpiI	GAAGAC	4 cut(s)	700, 1011, 1025, 1901
BplI	GAGNNNNNCTC	2 cut(s)	461, 493
Bpu1102I	GCTNAGC	1 cut(s)	1577
BpuEI	CTTGAG	2 cut(s)	469, 1126
BsaBI	GATNNNNATC	1 cut(s)	1692
BsaI	GGTCTC	2 cut(s)	557, 941
BsaJI	CCNNGG	5 cut(s)	749, 921, 1219, 1847, 1982
Bsc4I	CCNNNNNNNGG	1 cut(s)	1554
Bse1I	ACTGG	1 cut(s)	1716
Bse3DI	GCAATG	4 cut(s)	1238, 1437, 1725, 2007
Bse8I	GATNNNNATC	1 cut(s)	1692
BseBI	CCWGG	2 cut(s)	869, 1818
BseDI	CCNNGG	5 cut(s)	749, 921, 1219, 1847, 1982
BseGI	GGATG	3 cut(s)	1018, 1181, 1660
BseJI	GATNNNNATC	1 cut(s)	1692
BseLI	CCNNNNNNNGG	1 cut(s)	1554
BseMI	GCAATG	4 cut(s)	1238, 1437, 1725, 2007
BseMII	CTCAG	8 cut(s)	492, 936, 1264, 1329, 1535, 1613, 1740, 1794
BseNI	ACTGG	1 cut(s)	1716
BseYI	CCCAGC	2 cut(s)	493, 1878
BsgI	GTGCAG	1 cut(s)	537
Bsh1236I	CGCG	2 cut(s)	414, 1044
BshFI	GGCC	4 cut(s)	419, 1149, 1296, 1821
BslFI	GGGAC	2 cut(s)	447, 1751
BslI	CCNNNNNNNGG	1 cut(s)	1554
BsmAI	GTCTC	3 cut(s)	557, 602, 941
BsmFI	GGGAC	2 cut(s)	447, 1751
BsmI	GAATGC	2 cut(s)	1197, 1213
BsnI	GGCC	4 cut(s)	419, 1149, 1296, 1821
Bso31I	GGTCTC	2 cut(s)	557, 941
Bsp1720I	GCTNAGC	1 cut(s)	1577
Bsp19I	CCATGG	1 cut(s)	749
Bsp68I	TCGCGA	1 cut(s)	414
BspACI	CCGC	4 cut(s)	565, 809, 1405, 1593
BspANI	GGCC	4 cut(s)	419, 1149, 1296, 1821
BspCNI	CTCAG	8 cut(s)	491, 937, 1263, 1328, 1534, 1612, 1741, 1795
BspFNI	CGCG	2 cut(s)	414, 1044
BspHI	TCATGA	1 cut(s)	1735
BspLI	GGNNCC	4 cut(s)	569, 675, 1297, 1533
BspMAI	CTGCAG	2 cut(s)	640, 1003
BspMI	ACCTGC	1 cut(s)	539
BspPI	GGATC	8 cut(s)	331, 339, 912, 1402, 1526, 1539, 1733, 1790
BspTNI	GGTCTC	2 cut(s)	557, 941
BsrDI	GCAATG	4 cut(s)	1238, 1437, 1725, 2007
BsrI	ACTGG	1 cut(s)	1716
BssECI	CCNNGG	5 cut(s)	749, 921, 1219, 1847, 1982
BssNAI	GTATAC	1 cut(s)	1360
BssSI	CACGAG	1 cut(s)	1889
BssT1I	CCWWGG	3 cut(s)	749, 1219, 1982
Bst1107I	GTATAC	1 cut(s)	1360
Bst2BI	CACGAG	1 cut(s)	1889
Bst2UI	CCWGG	2 cut(s)	869, 1818
Bst4CI	ACNGT	6 cut(s)	29, 58, 127, 136, 289, 1257
BstAPI	GCANNNNNTGC	2 cut(s)	662, 1565
BstC8I	GCNNGC	6 cut(s)	523, 1381, 1476, 1648, 1660, 2019
BstDSI	CCRYGG	2 cut(s)	749, 921
BstEII	GGTNACC	1 cut(s)	497
BstF5I	GGATG	3 cut(s)	1018, 1181, 1660
BstFNI	CGCG	2 cut(s)	414, 1044
BstHHI	GCGC	1 cut(s)	304
BstMAI	GTCTC	3 cut(s)	557, 602, 941
BstNI	CCWGG	2 cut(s)	869, 1818
BstNSI	RCATGY	2 cut(s)	1283, 1335
BstPI	GGTNACC	1 cut(s)	497
BstSCI	CCNGG	2 cut(s)	867, 1816
BstSFI	CTRYAG	2 cut(s)	636, 999
BstUI	CGCG	2 cut(s)	414, 1044
BstV2I	GAAGAC	4 cut(s)	700, 1011, 1025, 1901
BstX2I	RGATCY	9 cut(s)	323, 344, 640, 917, 958, 1111, 1394, 1531, 1772
BstYI	RGATCY	9 cut(s)	323, 344, 640, 917, 958, 1111, 1394, 1531, 1772
BstZ17I	GTATAC	1 cut(s)	1360
BsuRI	GGCC	4 cut(s)	419, 1149, 1296, 1821
BtgI	CCRYGG	2 cut(s)	749, 921
BtgZI	GCGATG	1 cut(s)	1059
BtrI	CACGTC	1 cut(s)	1481
BtsCI	GGATG	3 cut(s)	1018, 1181, 1660
BtsI	GCAGTG	1 cut(s)	1563
BtsIMutI	CAGTG	3 cut(s)	63, 1563, 1723
BtuMI	TCGCGA	1 cut(s)	414
BveI	ACCTGC	1 cut(s)	539
Cac8I	GCNNGC	6 cut(s)	523, 1381, 1476, 1648, 1660, 2019
CaiI	CAGNNNCTG	1 cut(s)	662
CciI	TCATGA	1 cut(s)	1735
CfoI	GCGC	1 cut(s)	304
Cfr13I	GGNCC	3 cut(s)	674, 1295, 1595
Csp6I	GTAC	4 cut(s)	37, 91, 137, 845
CspCI	CAANNNNNGTGG	2 cut(s)	29, 64
CviQI	GTAC	4 cut(s)	37, 91, 137, 845
DraI	TTTAAA	1 cut(s)	336
DraIII	CACNNNGTG	1 cut(s)	291
Eco130I	CCWWGG	3 cut(s)	749, 1219, 1982
Eco147I	AGGCCT	2 cut(s)	419, 1821
Eco31I	GGTCTC	2 cut(s)	557, 941
Eco47I	GGWCC	2 cut(s)	674, 1595
Eco57I	CTGAAG	2 cut(s)	636, 1524
Eco91I	GGTNACC	1 cut(s)	497
EcoO65I	GGTNACC	1 cut(s)	497
EcoRII	CCWGG	2 cut(s)	867, 1816
EcoT14I	CCWWGG	3 cut(s)	749, 1219, 1982
EcoT22I	ATGCAT	2 cut(s)	373, 1333
ErhI	CCWWGG	3 cut(s)	749, 1219, 1982
FalI	AAGNNNNNCTT	2 cut(s)	685, 717
FaqI	GGGAC	2 cut(s)	447, 1751
FauNDI	CATATG	1 cut(s)	1291
FbaI	TGATCA	2 cut(s)	282, 400
FblI	GTMKAC	1 cut(s)	1359
FokI	GGATG	3 cut(s)	1025, 1188, 1667
FspBI	CTAG	3 cut(s)	1220, 1244, 2029
GlaI	GCGC	1 cut(s)	303
GsaI	CCCAGC	2 cut(s)	497, 1882
HaeIII	GGCC	4 cut(s)	419, 1149, 1296, 1821
HhaI	GCGC	1 cut(s)	304
Hin6I	GCGC	1 cut(s)	302
HinP1I	GCGC	1 cut(s)	302
HincII	GTYRAC	2 cut(s)	181, 865
HindII	GTYRAC	2 cut(s)	181, 865
HindIII	AAGCTT	2 cut(s)	1574, 1911
HpaI	GTTAAC	1 cut(s)	865
Hpy166II	GTNNAC	6 cut(s)	111, 181, 865, 1312, 1360, 1888
Hpy188I	TCNGA	9 cut(s)	537, 1138, 1253, 1339, 1509, 1543, 1750, 1777, 2025
Hpy188III	TCNNGA	4 cut(s)	413, 1736, 1970, 1994
Hpy8I	GTNNAC	6 cut(s)	111, 181, 865, 1312, 1360, 1888
HpyAV	CCTTC	3 cut(s)	1124, 1160, 1310
HpyCH4III	ACNGT	6 cut(s)	29, 58, 127, 136, 289, 1257
HpyCH4IV	ACGT	1 cut(s)	1480
HpySE526I	ACGT	1 cut(s)	1480
HspAI	GCGC	1 cut(s)	302
Ksp22I	TGATCA	2 cut(s)	282, 400
KspAI	GTTAAC	1 cut(s)	865
LmnI	GCTCC	3 cut(s)	1169, 1682, 2025
LweI	GCATC	8 cut(s)	164, 218, 358, 916, 1534, 1645, 1711, 1987
MaeI	CTAG	3 cut(s)	1220, 1244, 2029
MaeII	ACGT	1 cut(s)	1480
MaeIII	GTNAC	5 cut(s)	497, 758, 1785, 1953, 1991
MflI	RGATCY	9 cut(s)	323, 344, 640, 917, 958, 1111, 1394, 1531, 1772
MlyI	GAGTC	8 cut(s)	106, 447, 748, 1133, 1242, 1307, 1513, 1893
MmeI	TCCRAC	1 cut(s)	1372
Mph1103I	ATGCAT	2 cut(s)	373, 1333
MseI	TTAA	4 cut(s)	335, 864, 1469, 1491
MspA1I	CMGCKG	1 cut(s)	809
MspR9I	CCNGG	2 cut(s)	869, 1818
Mva1269I	GAATGC	2 cut(s)	1197, 1213
MvaI	CCWGG	2 cut(s)	869, 1818
MvnI	CGCG	2 cut(s)	414, 1044
NcoI	CCATGG	1 cut(s)	749
NdeI	CATATG	1 cut(s)	1291
NlaIV	GGNNCC	4 cut(s)	569, 675, 1297, 1533
NmeAIII	GCCGAG	2 cut(s)	1594, 1872
NmuCI	GTSAC	4 cut(s)	497, 1785, 1953, 1991
NruI	TCGCGA	1 cut(s)	414
NsiI	ATGCAT	2 cut(s)	373, 1333
NspI	RCATGY	2 cut(s)	1283, 1335
PagI	TCATGA	1 cut(s)	1735
PceI	AGGCCT	2 cut(s)	419, 1821
PctI	GAATGC	2 cut(s)	1197, 1213
PfeI	GAWTC	2 cut(s)	8, 1454
PleI	GAGTC	8 cut(s)	106, 446, 748, 1133, 1242, 1307, 1513, 1892
PpsI	GAGTC	8 cut(s)	106, 446, 748, 1133, 1242, 1307, 1513, 1892
Psp6I	CCWGG	2 cut(s)	867, 1816
PspEI	GGTNACC	1 cut(s)	497
PspFI	CCCAGC	2 cut(s)	493, 1878
PspGI	CCWGG	2 cut(s)	867, 1816
PspN4I	GGNNCC	4 cut(s)	569, 675, 1297, 1533
PspPI	GGNCC	3 cut(s)	674, 1295, 1595
PstI	CTGCAG	2 cut(s)	640, 1003
PstNI	CAGNNNCTG	1 cut(s)	662
PsuI	RGATCY	9 cut(s)	323, 344, 640, 917, 958, 1111, 1394, 1531, 1772
RruI	TCGCGA	1 cut(s)	414
RsaI	GTAC	4 cut(s)	38, 92, 138, 846
RsaNI	GTAC	4 cut(s)	37, 91, 137, 845
SaqAI	TTAA	4 cut(s)	335, 864, 1469, 1491
Sau96I	GGNCC	3 cut(s)	674, 1295, 1595
ScaI	AGTACT	1 cut(s)	138
SchI	GAGTC	8 cut(s)	106, 447, 748, 1133, 1242, 1307, 1513, 1893
ScrFI	CCNGG	2 cut(s)	869, 1818
SfaNI	GCATC	8 cut(s)	164, 218, 358, 916, 1534, 1645, 1711, 1987
SfcI	CTRYAG	2 cut(s)	636, 999
SinI	GGWCC	2 cut(s)	674, 1595
SmlI	CTYRAG	2 cut(s)	448, 1141
SmoI	CTYRAG	2 cut(s)	448, 1141
SseBI	AGGCCT	2 cut(s)	419, 1821
SsiI	CCGC	4 cut(s)	565, 809, 1405, 1593
SspI	AATATT	1 cut(s)	247
SspMI	CTAG	3 cut(s)	1220, 1244, 2029
StuI	AGGCCT	2 cut(s)	419, 1821
StyD4I	CCNGG	2 cut(s)	867, 1816
StyI	CCWWGG	3 cut(s)	749, 1219, 1982
TaaI	ACNGT	6 cut(s)	29, 58, 127, 136, 289, 1257
TaiI	ACGT	1 cut(s)	1483
TaqI	TCGA	4 cut(s)	115, 487, 1857, 1971
TatI	WGTACW	1 cut(s)	136
TauI	GCSGC	2 cut(s)	812, 1408
TfiI	GAWTC	2 cut(s)	8, 1454
Tru1I	TTAA	4 cut(s)	335, 864, 1469, 1491
Tru9I	TTAA	4 cut(s)	335, 864, 1469, 1491
TscAI	CASTG	3 cut(s)	63, 1570, 1723
TseFI	GTSAC	4 cut(s)	497, 1785, 1953, 1991
Tsp45I	GTSAC	4 cut(s)	497, 1785, 1953, 1991
TspDTI	ATGAA	3 cut(s)	635, 1383, 1820
TspGWI	ACGGA	1 cut(s)	910
TspRI	CASTG	3 cut(s)	63, 1570, 1723
VpaK11BI	GGWCC	2 cut(s)	674, 1595
XapI	RAATTY	3 cut(s)	1273, 1340, 1370
XceI	RCATGY	2 cut(s)	1283, 1335
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1085
XmaJI	CCTAGG	1 cut(s)	1219
XmiI	GTMKAC	1 cut(s)	1359
XspI	CTAG	3 cut(s)	1220, 1244, 2029
ZrmI	AGTACT	1 cut(s)	138
Zsp2I	ATGCAT	2 cut(s)	373, 1333

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.