Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0003433

Physical Location & Seq

Genomic Coordinates Forward (+)

35436837 .. 35450260

Gene Structure 13424 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG161400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1845 bp

ATGATCTACTCTCAAAACAAAGCATCAAGTTATTGTGTTCTGGTGGCAACTTCAAGCAATATTTTGTGGAAGCAACAACTACTCATCTTTTTTGTGATCACGGTGTGTTTGGCTGCGCTGCTACAATGTTTGACTGGATCTAATGTTTTAAAATGGAGATCCTTTTATGCAACCCAAGATGATGCATGGAAAGCTCATTACAGGGAGGTATTTGATCATGGTATTCGCGAGGCCTTGTGCTGTATGGGACGAGTCAAATACTTGAGTGTATTGGAAGAAGATGAGGTTTTCTCAGTAGCTCGACTGCTGGGTGACCTTGTTGCCTATCGTGCAGCAGGCACAGGACATCTGAAACTTATGGCAGGTCTCGCACTATTGCGGAACCAGAGCCATTCACCAAAATCCTTTGACGAGTGCATGGAGACACCTGAAGAAAAGATCAGGGAGGCTGCAGATCTTCATAAGTTTGCAGAAGCTGCCTACACGGGTCCATTACTTGATTTTGGAAGACACCCTTTCTTATTTCCTTGTGCTTGGCTTTATAGGCAAGGGATTTTGACCCCATGGACTCGTAACAGGCTACCCAAACTTGATGGTGATAATTGGTGGCGAGGTCATGCAGCGGCGTTTCTAAAATATGTAAAGTTATCCCCAGAGGTACTTAGACAAGGGCGTGTTAACCAGGCGAAGTGTAGAGCTGCATACTTTGTTATAGTGCTGCATCATCTAAGATCCGTGGTGATTGCTGTTCGGGGAACTGAGACCCCAGAAGATCTAATTACTGACAGCTTATGTAGGGAATGTCGTCTTTCTGCAGAAGACTTGGATGGATTGATAAATAGCTGTCTTCTTCAACGCGATGTAAAAGAGAGCTTGATGTCTTTCCCTCACCACGCACACTCTGGTATAGTTGAGGCTGCAAGAGATCTTTTTATGCAAATTGAAGGCTCTGACTCAAGTGGCCTTCTTTCTTCTTTATTGGGAGCTGGATGTGAGTGTGAGGGATATAGCATTCGCATAGTGGGGCATTCCCTAGGAGGTGCGATTGGTGCATTGCTAGGACTCAGACTGTATCACCGATACCCAAATTTGCATGTCTATACATATGGCCCCCTTCCATGTGTGGACTCAGTTGTAGCAAATGCATGTTCTGAATTTATTACAAGCATTGTATACAACAATGAATTTTCAGCACGCCTTTCTGTTGGATCTCTCCTGCGGCTTCAAGGTGCTGCAATTATGGCAATGTCAAAAGATACAGAAGCCGATTCAGCTATGATATTAAGGCTTGCACGTCATTTTCTTAATGTAAGCAAACATCAGAAAGATAGGACTCAGGTAAAGGATCCAGCTTCAGATGCCATTTCTGGGGCAATCACTGCCGAGAAGCTTAGCCACCAAATCTGCGGACCTCAGTTTGATATAAAAGCAAGTGAGGAACAAGATGAGGGTTTCCTTGCATGCAAGGATGCTCGCACAGGTGAAACAGATTTTGGAGCTGATACCGATCAATTTACTAACCCTTTCGCCAGTGATGCCATTGCAAATCATGATCCTGTGTCTGAGTTTATGGAAAGTGTCCCCAGATCTGATAGTGTGTCACCTACCGATCCCCCTGAGATGTATCTGCCAGGCCTTGTAATTCATATAGTAGCACAGCCGAGGAACTTCAATATGCCTATTTGGAAAGGCTGGGGAGTCCACGAGAAGACAAAATCTCACAAAGCTTATATTGCAGACAGAGAAAGTTTCAAAGATATGATTGTGTCACCATCAATGTTTCTCGACCATCTTCCTTGGAGATGTCACGATGCAATGCTAAAATTATTGCAAGCTCGGAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0001676 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006690 GO:0007275 GO:0007399 GO:0007405 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009987 GO:0016020 GO:0019369 GO:0019752 GO:0022008 GO:0030154 GO:0032501 GO:0032502 GO:0032787 GO:0033559 GO:0042133 GO:0042136 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044424 GO:0044464 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0061351 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0072089 GO:1901568

KEGG Pathways

Metabolic & Signaling

ko04723 ko04745 ko04925 map04723 map04745 map04925

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

614

Amino Acids

68.46

Weight (kDa)

6.32

Isoelectric Point (pI)

42.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7358	PF24057	4 - 109	9.5e-54	Domain of unknown function (DUF7358)
Lipase_3	PF01764	247 - 396	2.3e-12	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003433)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05790 AT1G05790 AT1G05790 AT1G05790
fragaria_vesca	FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690 FvH4_4g11690
malus_domestica	MD04G1032500.v1.1 MD04G1032600.v1.1
prunus_persica	Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1 Prupe.1G181900_v2.0.a1
pyrus_communis	pycom04g02680 pycom04g02690
rosa_chinensis	RchiOBHm_Chr4g0408981
rosa_laevigata	RLG00000008601
rosa_multiflora	Rmu_sc0003685.1_g000003 Rmu_sc0031886.1_g000002
rosa_roxburghii	Rroxscaffold_5G00353410
rosa_rugosa	Rorug04G0088900 Rorug04G0088900
rosa_samantha	Rh4AG153000 Rh4BG150900 Rh4CG161400 Rh4DG146400

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	353
AccI	GTMKAC	1 cut(s)	1173
AccII	CGCG	2 cut(s)	228, 858
AciI	CCGC	4 cut(s)	379, 623, 1219, 1407
AclWI	GGATC	8 cut(s)	145, 153, 726, 1216, 1340, 1353, 1547, 1604
AcsI	RAATTY	3 cut(s)	1087, 1154, 1184
AcuI	CTGAAG	2 cut(s)	450, 1338
AdeI	CACNNNGTG	1 cut(s)	105
AfaI	GTAC	1 cut(s)	660
AfiI	CCNNNNNNNGG	1 cut(s)	1368
AgsI	TTSAA	6 cut(s)	54, 854, 944, 1226, 1672, 1753
AjiI	CACGTC	1 cut(s)	1295
AjnI	CCWGG	2 cut(s)	681, 1630
AjuI	GAANNNNNNNTTGG	2 cut(s)	1476, 1508
Alw26I	GTCTC	3 cut(s)	371, 416, 755
AlwI	GGATC	8 cut(s)	145, 153, 726, 1216, 1340, 1353, 1547, 1604
AlwNI	CAGNNNCTG	1 cut(s)	476
AoxI	GGCC	4 cut(s)	231, 961, 1108, 1633
ApoI	RAATTY	3 cut(s)	1087, 1154, 1184
AspA2I	CCTAGG	1 cut(s)	1033
AspLEI	GCGC	1 cut(s)	118
AspS9I	GGNCC	3 cut(s)	488, 1109, 1409
AsuHPI	GGTGA	9 cut(s)	323, 387, 608, 751, 881, 1067, 1493, 1593, 1761
AvaII	GGWCC	2 cut(s)	488, 1409
AvrII	CCTAGG	1 cut(s)	1033
BamHI	GGATCC	1 cut(s)	1345
BauI	CACGAG	1 cut(s)	1703
BbsI	GAAGAC	4 cut(s)	514, 825, 839, 1715
BccI	CCATC	4 cut(s)	587, 821, 1780, 1797
BciT130I	CCWGG	2 cut(s)	683, 1632
BclI	TGATCA	2 cut(s)	96, 214
BcoDI	GTCTC	3 cut(s)	371, 416, 755
BfaI	CTAG	3 cut(s)	1034, 1058, 1843
BfmI	CTRYAG	2 cut(s)	450, 813
BfuAI	ACCTGC	1 cut(s)	353
BglII	AGATCT	4 cut(s)	454, 772, 925, 1586
BlnI	CCTAGG	1 cut(s)	1033
BlpI	GCTNAGC	1 cut(s)	1391
Bme1390I	CCNGG	2 cut(s)	683, 1632
Bme18I	GGWCC	2 cut(s)	488, 1409
BmgBI	CACGTC	1 cut(s)	1295
BmgT120I	GGNCC	3 cut(s)	488, 1109, 1409
BmiI	GGNNCC	4 cut(s)	383, 489, 1111, 1347
BmrFI	CCNGG	2 cut(s)	683, 1632
BmsI	GCATC	7 cut(s)	32, 172, 730, 1348, 1459, 1525, 1801
BpiI	GAAGAC	4 cut(s)	514, 825, 839, 1715
BplI	GAGNNNNNCTC	2 cut(s)	275, 307
Bpu1102I	GCTNAGC	1 cut(s)	1391
BpuEI	CTTGAG	2 cut(s)	283, 940
BsaBI	GATNNNNATC	1 cut(s)	1506
BsaI	GGTCTC	2 cut(s)	371, 755
BsaJI	CCNNGG	5 cut(s)	563, 735, 1033, 1661, 1796
Bsc4I	CCNNNNNNNGG	1 cut(s)	1368
Bse1I	ACTGG	2 cut(s)	139, 1530
Bse3DI	GCAATG	4 cut(s)	1052, 1251, 1539, 1821
Bse8I	GATNNNNATC	1 cut(s)	1506
BseBI	CCWGG	2 cut(s)	683, 1632
BseDI	CCNNGG	5 cut(s)	563, 735, 1033, 1661, 1796
BseGI	GGATG	3 cut(s)	832, 995, 1474
BseJI	GATNNNNATC	1 cut(s)	1506
BseLI	CCNNNNNNNGG	1 cut(s)	1368
BseMI	GCAATG	4 cut(s)	1052, 1251, 1539, 1821
BseMII	CTCAG	8 cut(s)	306, 750, 1078, 1143, 1349, 1427, 1554, 1608
BseNI	ACTGG	2 cut(s)	139, 1530
BseYI	CCCAGC	2 cut(s)	307, 1692
BsgI	GTGCAG	1 cut(s)	351
Bsh1236I	CGCG	2 cut(s)	228, 858
BshFI	GGCC	4 cut(s)	233, 963, 1110, 1635
BslFI	GGGAC	2 cut(s)	261, 1565
BslI	CCNNNNNNNGG	1 cut(s)	1368
BsmAI	GTCTC	3 cut(s)	371, 416, 755
BsmFI	GGGAC	2 cut(s)	261, 1565
BsmI	GAATGC	2 cut(s)	1011, 1027
BsnI	GGCC	4 cut(s)	233, 963, 1110, 1635
Bso31I	GGTCTC	2 cut(s)	371, 755
Bsp1720I	GCTNAGC	1 cut(s)	1391
Bsp19I	CCATGG	1 cut(s)	563
Bsp68I	TCGCGA	1 cut(s)	228
BspACI	CCGC	4 cut(s)	379, 623, 1219, 1407
BspANI	GGCC	4 cut(s)	233, 963, 1110, 1635
BspCNI	CTCAG	8 cut(s)	305, 751, 1077, 1142, 1348, 1426, 1555, 1609
BspFNI	CGCG	2 cut(s)	228, 858
BspHI	TCATGA	1 cut(s)	1549
BspLI	GGNNCC	4 cut(s)	383, 489, 1111, 1347
BspMAI	CTGCAG	2 cut(s)	454, 817
BspMI	ACCTGC	1 cut(s)	353
BspPI	GGATC	8 cut(s)	145, 153, 726, 1216, 1340, 1353, 1547, 1604
BspTNI	GGTCTC	2 cut(s)	371, 755
BsrDI	GCAATG	4 cut(s)	1052, 1251, 1539, 1821
BsrI	ACTGG	2 cut(s)	139, 1530
BssECI	CCNNGG	5 cut(s)	563, 735, 1033, 1661, 1796
BssNAI	GTATAC	1 cut(s)	1174
BssSI	CACGAG	1 cut(s)	1703
BssT1I	CCWWGG	3 cut(s)	563, 1033, 1796
Bst1107I	GTATAC	1 cut(s)	1174
Bst2BI	CACGAG	1 cut(s)	1703
Bst2UI	CCWGG	2 cut(s)	683, 1632
Bst4CI	ACNGT	2 cut(s)	103, 1071
BstAPI	GCANNNNNTGC	2 cut(s)	476, 1379
BstC8I	GCNNGC	6 cut(s)	337, 1195, 1290, 1462, 1474, 1833
BstDSI	CCRYGG	2 cut(s)	563, 735
BstEII	GGTNACC	1 cut(s)	311
BstF5I	GGATG	3 cut(s)	832, 995, 1474
BstFNI	CGCG	2 cut(s)	228, 858
BstHHI	GCGC	1 cut(s)	118
BstMAI	GTCTC	3 cut(s)	371, 416, 755
BstNI	CCWGG	2 cut(s)	683, 1632
BstNSI	RCATGY	3 cut(s)	1097, 1149, 1464
BstPI	GGTNACC	1 cut(s)	311
BstSCI	CCNGG	2 cut(s)	681, 1630
BstSFI	CTRYAG	2 cut(s)	450, 813
BstUI	CGCG	2 cut(s)	228, 858
BstV2I	GAAGAC	4 cut(s)	514, 825, 839, 1715
BstX2I	RGATCY	9 cut(s)	137, 158, 454, 731, 772, 925, 1208, 1345, 1586
BstYI	RGATCY	9 cut(s)	137, 158, 454, 731, 772, 925, 1208, 1345, 1586
BstZ17I	GTATAC	1 cut(s)	1174
BsuRI	GGCC	4 cut(s)	233, 963, 1110, 1635
BtgI	CCRYGG	2 cut(s)	563, 735
BtgZI	GCGATG	1 cut(s)	873
BtrI	CACGTC	1 cut(s)	1295
BtsCI	GGATG	3 cut(s)	832, 995, 1474
BtsI	GCAGTG	1 cut(s)	1377
BtsIMutI	CAGTG	2 cut(s)	1377, 1537
BtuMI	TCGCGA	1 cut(s)	228
BveI	ACCTGC	1 cut(s)	353
Cac8I	GCNNGC	6 cut(s)	337, 1195, 1290, 1462, 1474, 1833
CaiI	CAGNNNCTG	1 cut(s)	476
CciI	TCATGA	1 cut(s)	1549
CfoI	GCGC	1 cut(s)	118
Cfr13I	GGNCC	3 cut(s)	488, 1109, 1409
Csp6I	GTAC	1 cut(s)	659
CviQI	GTAC	1 cut(s)	659
DraI	TTTAAA	1 cut(s)	150
DraIII	CACNNNGTG	1 cut(s)	105
Eco130I	CCWWGG	3 cut(s)	563, 1033, 1796
Eco147I	AGGCCT	2 cut(s)	233, 1635
Eco31I	GGTCTC	2 cut(s)	371, 755
Eco47I	GGWCC	2 cut(s)	488, 1409
Eco57I	CTGAAG	2 cut(s)	450, 1338
Eco91I	GGTNACC	1 cut(s)	311
EcoO65I	GGTNACC	1 cut(s)	311
EcoRII	CCWGG	2 cut(s)	681, 1630
EcoT14I	CCWWGG	3 cut(s)	563, 1033, 1796
EcoT22I	ATGCAT	2 cut(s)	187, 1147
ErhI	CCWWGG	3 cut(s)	563, 1033, 1796
FalI	AAGNNNNNCTT	2 cut(s)	499, 531
FaqI	GGGAC	2 cut(s)	261, 1565
FauNDI	CATATG	1 cut(s)	1105
FbaI	TGATCA	2 cut(s)	96, 214
FblI	GTMKAC	1 cut(s)	1173
FokI	GGATG	3 cut(s)	839, 1002, 1481
FspBI	CTAG	3 cut(s)	1034, 1058, 1843
GlaI	GCGC	1 cut(s)	117
GsaI	CCCAGC	2 cut(s)	311, 1696
HaeIII	GGCC	4 cut(s)	233, 963, 1110, 1635
HhaI	GCGC	1 cut(s)	118
Hin6I	GCGC	1 cut(s)	116
HinP1I	GCGC	1 cut(s)	116
HincII	GTYRAC	1 cut(s)	679
HindII	GTYRAC	1 cut(s)	679
HindIII	AAGCTT	2 cut(s)	1388, 1725
HinfI	GANTC	8 cut(s)	252, 568, 953, 1062, 1127, 1268, 1333, 1698
HpaI	GTTAAC	1 cut(s)	679
HphI	GGTGA	9 cut(s)	323, 387, 608, 751, 881, 1067, 1493, 1593, 1761
Hpy166II	GTNNAC	4 cut(s)	679, 1126, 1174, 1702
Hpy188I	TCNGA	9 cut(s)	351, 952, 1067, 1153, 1323, 1357, 1564, 1591, 1839
Hpy188III	TCNNGA	4 cut(s)	227, 1550, 1784, 1808
Hpy8I	GTNNAC	4 cut(s)	679, 1126, 1174, 1702
HpyAV	CCTTC	3 cut(s)	938, 974, 1124
HpyCH4III	ACNGT	2 cut(s)	103, 1071
HpyCH4IV	ACGT	1 cut(s)	1294
HpySE526I	ACGT	1 cut(s)	1294
HspAI	GCGC	1 cut(s)	116
Ksp22I	TGATCA	2 cut(s)	96, 214
KspAI	GTTAAC	1 cut(s)	679
LmnI	GCTCC	3 cut(s)	983, 1496, 1839
LweI	GCATC	7 cut(s)	32, 172, 730, 1348, 1459, 1525, 1801
MaeI	CTAG	3 cut(s)	1034, 1058, 1843
MaeII	ACGT	1 cut(s)	1294
MaeIII	GTNAC	5 cut(s)	311, 572, 1599, 1767, 1805
MflI	RGATCY	9 cut(s)	137, 158, 454, 731, 772, 925, 1208, 1345, 1586
MlyI	GAGTC	7 cut(s)	261, 562, 947, 1056, 1121, 1327, 1707
MmeI	TCCRAC	1 cut(s)	1186
Mph1103I	ATGCAT	2 cut(s)	187, 1147
MseI	TTAA	4 cut(s)	149, 678, 1283, 1305
MspA1I	CMGCKG	1 cut(s)	623
MspR9I	CCNGG	2 cut(s)	683, 1632
Mva1269I	GAATGC	2 cut(s)	1011, 1027
MvaI	CCWGG	2 cut(s)	683, 1632
MvnI	CGCG	2 cut(s)	228, 858
NcoI	CCATGG	1 cut(s)	563
NdeI	CATATG	1 cut(s)	1105
NlaIV	GGNNCC	4 cut(s)	383, 489, 1111, 1347
NmeAIII	GCCGAG	2 cut(s)	1408, 1686
NmuCI	GTSAC	4 cut(s)	311, 1599, 1767, 1805
NruI	TCGCGA	1 cut(s)	228
NsiI	ATGCAT	2 cut(s)	187, 1147
NspI	RCATGY	3 cut(s)	1097, 1149, 1464
PaeI	GCATGC	1 cut(s)	1464
PagI	TCATGA	1 cut(s)	1549
PceI	AGGCCT	2 cut(s)	233, 1635
PctI	GAATGC	2 cut(s)	1011, 1027
PfeI	GAWTC	1 cut(s)	1268
PleI	GAGTC	7 cut(s)	260, 562, 947, 1056, 1121, 1327, 1706
PpsI	GAGTC	7 cut(s)	260, 562, 947, 1056, 1121, 1327, 1706
Psp6I	CCWGG	2 cut(s)	681, 1630
PspEI	GGTNACC	1 cut(s)	311
PspFI	CCCAGC	2 cut(s)	307, 1692
PspGI	CCWGG	2 cut(s)	681, 1630
PspN4I	GGNNCC	4 cut(s)	383, 489, 1111, 1347
PspPI	GGNCC	3 cut(s)	488, 1109, 1409
PstI	CTGCAG	2 cut(s)	454, 817
PstNI	CAGNNNCTG	1 cut(s)	476
PsuI	RGATCY	9 cut(s)	137, 158, 454, 731, 772, 925, 1208, 1345, 1586
RruI	TCGCGA	1 cut(s)	228
RsaI	GTAC	1 cut(s)	660
RsaNI	GTAC	1 cut(s)	659
SaqAI	TTAA	4 cut(s)	149, 678, 1283, 1305
Sau96I	GGNCC	3 cut(s)	488, 1109, 1409
SchI	GAGTC	7 cut(s)	261, 562, 947, 1056, 1121, 1327, 1707
ScrFI	CCNGG	2 cut(s)	683, 1632
SfaNI	GCATC	7 cut(s)	32, 172, 730, 1348, 1459, 1525, 1801
SfcI	CTRYAG	2 cut(s)	450, 813
SinI	GGWCC	2 cut(s)	488, 1409
SmlI	CTYRAG	2 cut(s)	262, 955
SmoI	CTYRAG	2 cut(s)	262, 955
SphI	GCATGC	1 cut(s)	1464
SseBI	AGGCCT	2 cut(s)	233, 1635
SsiI	CCGC	4 cut(s)	379, 623, 1219, 1407
SspI	AATATT	1 cut(s)	61
SspMI	CTAG	3 cut(s)	1034, 1058, 1843
StuI	AGGCCT	2 cut(s)	233, 1635
StyD4I	CCNGG	2 cut(s)	681, 1630
StyI	CCWWGG	3 cut(s)	563, 1033, 1796
TaaI	ACNGT	2 cut(s)	103, 1071
TaiI	ACGT	1 cut(s)	1297
TaqI	TCGA	2 cut(s)	301, 1785
TauI	GCSGC	2 cut(s)	626, 1222
TfiI	GAWTC	1 cut(s)	1268
Tru1I	TTAA	4 cut(s)	149, 678, 1283, 1305
Tru9I	TTAA	4 cut(s)	149, 678, 1283, 1305
TscAI	CASTG	2 cut(s)	1384, 1537
TseFI	GTSAC	4 cut(s)	311, 1599, 1767, 1805
Tsp45I	GTSAC	4 cut(s)	311, 1599, 1767, 1805
TspDTI	ATGAA	3 cut(s)	449, 1197, 1634
TspGWI	ACGGA	1 cut(s)	724
TspRI	CASTG	2 cut(s)	1384, 1537
VpaK11BI	GGWCC	2 cut(s)	488, 1409
XapI	RAATTY	3 cut(s)	1087, 1154, 1184
XceI	RCATGY	3 cut(s)	1097, 1149, 1464
XcmI	CCANNNNNNNNNTGG	1 cut(s)	899
XmaJI	CCTAGG	1 cut(s)	1033
XmiI	GTMKAC	1 cut(s)	1173
XspI	CTAG	3 cut(s)	1034, 1058, 1843
Zsp2I	ATGCAT	2 cut(s)	187, 1147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.