Rh4AG220800

Belongs to the adaptor complexes medium subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007388
Physical Location & Seq
Forward (+)
51931461 .. 51936391
4931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG220800.1

Sequence Viewer

Length: 1650 bp
ATGTGCAGAGAGGGCTCTATTCGTGGGTGTGGAATACGTGTAAGTCAGTCAGCTAAAGGCTCAGATTCTTGGGTTGATGATCCAATTACCCGTCATATAATTGGAATTTACATATGCAAAGAAAAGGAAGGGGATGGCAATAATGATAAAAATAATTTGCTGTGGCCTTTAGTCTTGCACACAAAGGGCCATTTTTGCATCCTGATATTGCCTTTAGTAGAGCCGAGGCATTTGAAGGCATATGCAAGATTATGTAATAGATCTGATTGTGGCAATGCTGTTGGTGTGGAGGACAATATATCTTCCATTCTGCTCGATCTTCCATCCATCACAGGGGCATTTATGGCGGCACATGCAATTGGTGACATAATAACTGGCGACGTCATGGAACCTGAGGTAGTTGTCAATGCGTCTCCATCTGTTGGAGGTTTATTGGATTCACTAACTGGCAGCATAGGAATATCGAGCATTTCCTCAAGGGCAAAACCTATTGCTGCACCAGTTGCATCCTCGAGCCCTACTAGCAGTGCTGTGACTGGAACTGTGACATCAGATGCTAACAAGATTGGTTCTAGGCCTTTAGATAAAGATGCACTTCGAACTTTCATAAGCAGTTCAATGCCCTTTGGAACGCTGATGGATTTGAGCTTCCCCAATATCATTTCCATTAAGGCTAATGGTTTTTCGTCATCAGATCTTCCTCCTTCAGACCTGAAGCAACCAGCATGGAAGCCTTACCTATACAAAGGAAGGCAGAGAATTCTATTTTCTATTCATGAGACTGTCCATGCTGCTATGTACGACCGAGATGAGATTCCAGATAGTATATTAATTTCTGGACATATAAATTGCCGAGCAGAGTTAGAAGGATTGCCTGATGTGTCCTTCCCCTTGATAGGACTGAACTCAGATAAGGTTGAAGTTTTATCATTTCATCCTTGTGCTCAAGTTCCAGAACAAGGTGTTGATAAGCAGGCTGTGATGTTTTCACCACCATTAGGTAATTTTGCTTTAATGCGTTACCAAGCAGTCTGTGGTATTGGACCACCCATAAAGGGATTTTACCAATTGTCTATGGTATCTGAGGATAAAGGTGATTTTTTATTCAAATTGCGGCTAATGGATGGTTACAAGTCTCCTCTCACAATGGAGTTTTGTACTGTGACTATGCCTTTTCCAAGGAGACGGGTTGTATCTTTTGATGGGACTCCCTCGGTCAGAATAGTTTCAACTACAGAGCATTCTGTTGAATGGAAGATTATAATGGGCGGGCGGGGTCTTAGTGGGAAAAGTATCGAGGCAACTTTTCCTGGTAAAGTCCAGTTTGCACCATGGAAACCCCAAATATTACCTCCGTCTAGTTTGGCCTTTGGAATCATAACTGATGAAGATAGTGATATTGAGACGGATGGTAATAATAATAGTATGGTCAATGTAGAGGAGTTCTTAATGGAGAAAATGAGCAAGGATCTTCATCCTGCCGATCTGGAGGAGCCATTTTGCTGGCATGCATACAACTATGCCAAGGTGTCCTTCAAAATTGTTGGAGCTTCATTGTCTGGAATGTCAATTGATCCGAAATCTGTAAGTTGTGACTCCTTTATTTATGCTCTCACATCATTACAGCAACCCAGAGATAAGGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adap_comp_sub

Protein Analysis

549

Amino Acids

59.76

Weight (kDa)

5.54

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 242 - 527 1.7e-27 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1262
AatII GACGTC 1 cut(s) 384
AccB7I CCANNNNNTGG 1 cut(s) 422
AciI CCGC 4 cut(s) 347, 1114, 1269, 1273
AclWI GGATC 3 cut(s) 74, 1476, 1568
AcsI RAATTY 2 cut(s) 105, 759
AcuI CTGAAG 2 cut(s) 690, 734
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 2 cut(s) 800, 1159
AfiI CCNNNNNNNGG 6 cut(s) 333, 422, 896, 959, 998, 1055
AflIII ACRYGT 1 cut(s) 37
AgsI TTSAA 7 cut(s) 235, 618, 920, 1108, 1230, 1250, 1537
AjnI CCWGG 1 cut(s) 1309
AjuI GAANNNNNNNTTGG 2 cut(s) 1517, 1549
AluBI AGCT 3 cut(s) 53, 648, 1550
AluI AGCT 3 cut(s) 53, 648, 1550
Alw21I GWGCWC 1 cut(s) 946
Alw26I GTCTC 5 cut(s) 417, 773, 1140, 1177, 1397
AlwI GGATC 3 cut(s) 74, 1476, 1568
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 4 cut(s) 164, 187, 575, 1365
ApeKI GCWGC 3 cut(s) 450, 494, 791
ApoI RAATTY 2 cut(s) 105, 759
AseI ATTAAT 1 cut(s) 830
Asp700I GAANNNNTTC 1 cut(s) 1225
AspS9I GGNCC 2 cut(s) 187, 1043
AsuHPI GGTGA 3 cut(s) 374, 981, 1106
AsuII TTCGAA 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 511
AvaII GGWCC 1 cut(s) 1043
AxyI CCTNAGG 1 cut(s) 393
BanII GRGCYC 2 cut(s) 17, 518
Bbv12I GWGCWC 1 cut(s) 946
BbvI GCAGC 3 cut(s) 462, 481, 778
BccI CCATC 8 cut(s) 128, 331, 335, 424, 631, 1118, 1196, 1403
BciT130I CCWGG 1 cut(s) 1311
BcoDI GTCTC 5 cut(s) 417, 773, 1140, 1177, 1397
BfaI CTAG 3 cut(s) 522, 573, 1359
BfmI CTRYAG 1 cut(s) 1233
BglII AGATCT 2 cut(s) 260, 694
BisI GCNGC 5 cut(s) 348, 451, 495, 792, 1115
BlsI GCNGC 5 cut(s) 349, 452, 496, 793, 1116
Bme1390I CCNGG 1 cut(s) 1311
Bme18I GGWCC 1 cut(s) 1043
BmeT110I CYCGRG 1 cut(s) 511
BmgT120I GGNCC 2 cut(s) 187, 1043
BmiI GGNNCC 2 cut(s) 390, 1494
BmrFI CCNGG 1 cut(s) 1311
BmsI GCATC 4 cut(s) 207, 515, 544, 580
BpmI CTGGAG 1 cut(s) 1508
Bpu14I TTCGAA 1 cut(s) 598
BpuEI CTTGAG 2 cut(s) 460, 930
BsaAI YACGTR 1 cut(s) 38
BsaBI GATNNNNATC 1 cut(s) 1473
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 5 cut(s) 224, 1178, 1212, 1331, 1524
Bsc4I CCNNNNNNNGG 6 cut(s) 333, 422, 896, 959, 998, 1055
Bse1I ACTGG 5 cut(s) 379, 451, 500, 541, 1321
Bse21I CCTNAGG 1 cut(s) 393
Bse3DI GCAATG 1 cut(s) 280
Bse8I GATNNNNATC 1 cut(s) 1473
BseBI CCWGG 1 cut(s) 1311
BseDI CCNNGG 5 cut(s) 224, 1178, 1212, 1331, 1524
BseGI GGATG 8 cut(s) 139, 198, 323, 506, 934, 1129, 1414, 1474
BseJI GATNNNNATC 1 cut(s) 1473
BseLI CCNNNNNNNGG 6 cut(s) 333, 422, 896, 959, 998, 1055
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 4 cut(s) 75, 384, 921, 1074
BseNI ACTGG 5 cut(s) 379, 451, 500, 541, 1321
BseRI GAGGAG 3 cut(s) 1128, 1454, 1505
BseXI GCAGC 3 cut(s) 462, 481, 778
BsgI GTGCAG 2 cut(s) 25, 480
Bsh1285I CGRYCG 1 cut(s) 805
BshFI GGCC 4 cut(s) 166, 189, 577, 1367
BsiEI CGRYCG 1 cut(s) 805
BsiHKAI GWGCWC 1 cut(s) 946
BsiHKCI CYCGRG 1 cut(s) 511
BslFI GGGAC 1 cut(s) 1219
BslI CCNNNNNNNGG 6 cut(s) 333, 422, 896, 959, 998, 1055
BsmAI GTCTC 5 cut(s) 417, 773, 1140, 1177, 1397
BsmBI CGTCTC 3 cut(s) 417, 1177, 1397
BsmFI GGGAC 1 cut(s) 1219
BsmI GAATGC 1 cut(s) 1240
BsnI GGCC 4 cut(s) 166, 189, 577, 1367
BsoBI CYCGRG 1 cut(s) 511
Bsp119I TTCGAA 1 cut(s) 598
Bsp1286I GDGCHC 3 cut(s) 17, 518, 946
Bsp143I GATC 7 cut(s) 79, 260, 316, 694, 1468, 1483, 1573
Bsp19I CCATGG 1 cut(s) 1331
BspACI CCGC 4 cut(s) 347, 1114, 1269, 1273
BspANI GGCC 4 cut(s) 166, 189, 577, 1367
BspCNI CTCAG 4 cut(s) 74, 385, 920, 1075
BspHI TCATGA 1 cut(s) 775
BspLI GGNNCC 2 cut(s) 390, 1494
BspPI GGATC 3 cut(s) 74, 1476, 1568
BspT104I TTCGAA 1 cut(s) 598
BsrDI GCAATG 1 cut(s) 280
BsrI ACTGG 5 cut(s) 379, 451, 500, 541, 1321
BssECI CCNNGG 5 cut(s) 224, 1178, 1212, 1331, 1524
BssMI GATC 7 cut(s) 79, 260, 316, 694, 1468, 1483, 1573
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 3 cut(s) 1178, 1331, 1524
Bst2UI CCWGG 1 cut(s) 1311
Bst4CI ACNGT 3 cut(s) 544, 784, 1162
BstACI GRCGYC 1 cut(s) 381
BstAPI GCANNNNNTGC 1 cut(s) 503
BstBAI YACGTR 1 cut(s) 38
BstBI TTCGAA 1 cut(s) 598
BstC8I GCNNGC 4 cut(s) 975, 1271, 1505, 1509
BstDEI CTNAG 5 cut(s) 61, 393, 907, 1083, 1280
BstDSI CCRYGG 1 cut(s) 1331
BstF5I GGATG 8 cut(s) 139, 198, 323, 506, 934, 1129, 1414, 1474
BstKTI GATC 7 cut(s) 82, 263, 319, 697, 1471, 1486, 1576
BstMAI GTCTC 5 cut(s) 417, 773, 1140, 1177, 1397
BstMBI GATC 7 cut(s) 79, 260, 316, 694, 1468, 1483, 1573
BstMCI CGRYCG 1 cut(s) 805
BstMWI GCNNNNNNNGC 6 cut(s) 12, 195, 344, 353, 503, 522
BstNI CCWGG 1 cut(s) 1311
BstNSI RCATGY 2 cut(s) 356, 1511
BstSCI CCNGG 1 cut(s) 1309
BstSFI CTRYAG 1 cut(s) 1233
BstV1I GCAGC 3 cut(s) 462, 481, 778
BstX2I RGATCY 3 cut(s) 260, 694, 1468
BstXI CCANNNNNNTGG 1 cut(s) 1503
BstYI RGATCY 3 cut(s) 260, 694, 1468
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 4 cut(s) 166, 189, 577, 1367
BtgI CCRYGG 1 cut(s) 1331
BtsCI GGATG 8 cut(s) 139, 198, 323, 506, 934, 1129, 1414, 1474
BtsI GCAGTG 1 cut(s) 532
BtsIMutI CAGTG 1 cut(s) 532
Cac8I GCNNGC 4 cut(s) 975, 1271, 1505, 1509
CciI TCATGA 1 cut(s) 775
Cfr13I GGNCC 2 cut(s) 187, 1043
CseI GACGC 1 cut(s) 399
Csp6I GTAC 2 cut(s) 799, 1158
CviAII CATG 7 cut(s) 353, 385, 726, 776, 788, 1332, 1508
CviQI GTAC 2 cut(s) 799, 1158
DdeI CTNAG 5 cut(s) 61, 393, 907, 1083, 1280
DpnI GATC 7 cut(s) 81, 262, 318, 696, 1470, 1485, 1575
DpnII GATC 7 cut(s) 79, 260, 316, 694, 1468, 1483, 1573
Eco130I CCWWGG 3 cut(s) 1178, 1331, 1524
Eco147I AGGCCT 1 cut(s) 577
Eco24I GRGCYC 2 cut(s) 17, 518
Eco47I GGWCC 1 cut(s) 1043
Eco57I CTGAAG 2 cut(s) 690, 734
Eco81I CCTNAGG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 511
EcoRI GAATTC 1 cut(s) 759
EcoRII CCWGG 1 cut(s) 1309
EcoT14I CCWWGG 3 cut(s) 1178, 1331, 1524
EcoT22I ATGCAT 1 cut(s) 1513
EcoT38I GRGCYC 2 cut(s) 17, 518
ErhI CCWWGG 3 cut(s) 1178, 1331, 1524
Esp3I CGTCTC 3 cut(s) 417, 1177, 1397
FaeI CATG 7 cut(s) 356, 388, 729, 779, 791, 1335, 1511
FalI AAGNNNNNCTT 4 cut(s) 579, 611, 1517, 1549
FaqI GGGAC 1 cut(s) 1219
FatI CATG 7 cut(s) 352, 384, 725, 775, 787, 1331, 1507
FauI CCCGC 2 cut(s) 1262, 1266
FauNDI CATATG 2 cut(s) 113, 241
Fnu4HI GCNGC 5 cut(s) 348, 451, 495, 792, 1115
FokI GGATG 8 cut(s) 146, 185, 310, 493, 921, 1136, 1421, 1461
FriOI GRGCYC 2 cut(s) 17, 518
Fsp4HI GCNGC 5 cut(s) 348, 451, 495, 792, 1115
FspBI CTAG 3 cut(s) 522, 573, 1359
GluI GCNGC 5 cut(s) 348, 451, 495, 792, 1115
GsuI CTGGAG 1 cut(s) 1508
HaeIII GGCC 4 cut(s) 166, 189, 577, 1367
HgaI GACGC 1 cut(s) 399
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 7 cut(s) 356, 388, 729, 779, 791, 1335, 1511
HinfI GANTC 6 cut(s) 65, 437, 814, 1207, 1374, 1595
HphI GGTGA 3 cut(s) 374, 981, 1106
Hpy188I TCNGA 9 cut(s) 64, 265, 553, 694, 709, 910, 1084, 1220, 1578
Hpy188III TCNNGA 7 cut(s) 202, 776, 818, 837, 953, 1487, 1560
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 7 cut(s) 122, 229, 714, 744, 860, 895, 1543
HpyCH4III ACNGT 3 cut(s) 544, 784, 1162
HpyCH4IV ACGT 2 cut(s) 37, 381
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 195, 344, 353, 503, 522
HpyF3I CTNAG 5 cut(s) 61, 393, 907, 1083, 1280
HpySE526I ACGT 2 cut(s) 37, 381
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 7 cut(s) 356, 388, 729, 779, 791, 1335, 1511
Kzo9I GATC 7 cut(s) 79, 260, 316, 694, 1468, 1483, 1573
LmnI GCTCC 2 cut(s) 1492, 1547
Lsp1109I GCAGC 3 cut(s) 462, 481, 778
LweI GCATC 4 cut(s) 207, 515, 544, 580
MaeI CTAG 3 cut(s) 522, 573, 1359
MaeII ACGT 2 cut(s) 37, 381
MaeIII GTNAC 7 cut(s) 362, 532, 544, 1019, 1127, 1162, 1592
MalI GATC 7 cut(s) 81, 262, 318, 696, 1470, 1485, 1575
MboI GATC 7 cut(s) 79, 260, 316, 694, 1468, 1483, 1573
MboII GAAGA 6 cut(s) 294, 311, 689, 1267, 1400, 1463
MfeI CAATTG 3 cut(s) 357, 1067, 1569
MflI RGATCY 3 cut(s) 260, 694, 1468
MhlI GDGCHC 3 cut(s) 17, 518, 946
MlyI GAGTC 2 cut(s) 1201, 1589
MmeI TCCRAC 2 cut(s) 403, 1525
Mph1103I ATGCAT 1 cut(s) 1513
MroXI GAANNNNTTC 1 cut(s) 1225
MseI TTAA 4 cut(s) 669, 830, 1013, 1448
MslI CAYNNNNRTG 1 cut(s) 939
MspR9I CCNGG 1 cut(s) 1311
MunI CAATTG 3 cut(s) 357, 1067, 1569
Mva1269I GAATGC 1 cut(s) 1240
MvaI CCWGG 1 cut(s) 1311
MwoI GCNNNNNNNGC 6 cut(s) 12, 195, 344, 353, 503, 522
NcoI CCATGG 1 cut(s) 1331
NdeI CATATG 2 cut(s) 113, 241
NdeII GATC 7 cut(s) 79, 260, 316, 694, 1468, 1483, 1573
NlaIII CATG 7 cut(s) 356, 388, 729, 779, 791, 1335, 1511
NlaIV GGNNCC 2 cut(s) 390, 1494
NmeAIII GCCGAG 2 cut(s) 249, 878
NmuCI GTSAC 5 cut(s) 362, 532, 544, 1162, 1592
NsiI ATGCAT 1 cut(s) 1513
NspI RCATGY 2 cut(s) 356, 1511
NspV TTCGAA 1 cut(s) 598
PaeI GCATGC 1 cut(s) 1511
PaeR7I CTCGAG 1 cut(s) 511
PagI TCATGA 1 cut(s) 775
PceI AGGCCT 1 cut(s) 577
PctI GAATGC 1 cut(s) 1240
PdmI GAANNNNTTC 1 cut(s) 1225
PfeI GAWTC 4 cut(s) 65, 437, 814, 1374
PflMI CCANNNNNTGG 1 cut(s) 422
PkrI GCNGC 5 cut(s) 349, 452, 496, 793, 1116
PleI GAGTC 2 cut(s) 1201, 1589
PpsI GAGTC 2 cut(s) 1201, 1589
Ppu21I YACGTR 1 cut(s) 38
PshBI ATTAAT 1 cut(s) 830
PsiI TTATAA 1 cut(s) 1262
Psp6I CCWGG 1 cut(s) 1309
PspGI CCWGG 1 cut(s) 1309
PspN4I GGNNCC 2 cut(s) 390, 1494
PspPI GGNCC 2 cut(s) 187, 1043
PspXI VCTCGAGB 1 cut(s) 511
PsuI RGATCY 3 cut(s) 260, 694, 1468
RsaI GTAC 2 cut(s) 800, 1159
RsaNI GTAC 2 cut(s) 799, 1158
RseI CAYNNNNRTG 1 cut(s) 939
SaqAI TTAA 4 cut(s) 669, 830, 1013, 1448
SatI GCNGC 5 cut(s) 348, 451, 495, 792, 1115
Sau3AI GATC 7 cut(s) 79, 260, 316, 694, 1468, 1483, 1573
Sau96I GGNCC 2 cut(s) 187, 1043
SchI GAGTC 2 cut(s) 1201, 1589
ScrFI CCNGG 1 cut(s) 1311
SduI GDGCHC 3 cut(s) 17, 518, 946
SfaNI GCATC 4 cut(s) 207, 515, 544, 580
SfcI CTRYAG 1 cut(s) 1233
Sfr274I CTCGAG 1 cut(s) 511
SfuI TTCGAA 1 cut(s) 598
SinI GGWCC 1 cut(s) 1043
SlaI CTCGAG 1 cut(s) 511
SmiMI CAYNNNNRTG 1 cut(s) 939
SmlI CTYRAG 3 cut(s) 475, 511, 945
SmoI CTYRAG 3 cut(s) 475, 511, 945
SphI GCATGC 1 cut(s) 1511
SseBI AGGCCT 1 cut(s) 577
SsiI CCGC 4 cut(s) 347, 1114, 1269, 1273
SspI AATATT 1 cut(s) 1347
SspMI CTAG 3 cut(s) 522, 573, 1359
StuI AGGCCT 1 cut(s) 577
StyD4I CCNGG 1 cut(s) 1309
StyI CCWWGG 3 cut(s) 1178, 1331, 1524
TaaI ACNGT 3 cut(s) 544, 784, 1162
TaiI ACGT 2 cut(s) 40, 384
TaqI TCGA 5 cut(s) 315, 464, 512, 598, 1296
TaqII GACCGA 2 cut(s) 819, 1204
TatI WGTACW 1 cut(s) 1157
TauI GCSGC 2 cut(s) 350, 1117
TfiI GAWTC 4 cut(s) 65, 437, 814, 1374
Tru1I TTAA 4 cut(s) 669, 830, 1013, 1448
Tru9I TTAA 4 cut(s) 669, 830, 1013, 1448
TscAI CASTG 1 cut(s) 532
TseFI GTSAC 5 cut(s) 362, 532, 544, 1162, 1592
TseI GCWGC 3 cut(s) 450, 494, 791
Tsp45I GTSAC 5 cut(s) 362, 532, 544, 1162, 1592
TspDTI ATGAA 6 cut(s) 595, 764, 923, 1401, 1463, 1542
TspGWI ACGGA 2 cut(s) 1344, 1421
TspRI CASTG 1 cut(s) 532
Van91I CCANNNNNTGG 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 1043
VspI ATTAAT 1 cut(s) 830
XapI RAATTY 2 cut(s) 105, 759
XceI RCATGY 2 cut(s) 356, 1511
XcmI CCANNNNNNNNNTGG 1 cut(s) 1031
XhoI CTCGAG 1 cut(s) 511
XmnI GAANNNNTTC 1 cut(s) 1225
XspI CTAG 3 cut(s) 522, 573, 1359
ZraI GACGTC 1 cut(s) 382
Zsp2I ATGCAT 1 cut(s) 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.