Rh4CG234100

Belongs to the adaptor complexes medium subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007388
Physical Location & Seq
Forward (+)
47657891 .. 47663836
5946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG234100.1

Sequence Viewer

Length: 1365 bp
ATGGCGGCACATGCAATTGGTGACATAATAACTGGCGACGTCGTGGAACCTGAGGTAGTTGTCAATGCATCTCCATCTGTTGGAGGTCTATTGGATTCGCTAACTGGCAGCATAGGAATATCGAGCATTTCCTCAAGGGCAAAACCAATTGCTGCACCAGTTGCATCCTCGAGCCCTACTAGCAGTGCTGTGACTGGAACTGTGACATCAGATGCTAACAAGACTGGTTCTAGGCCTTTAGATAAAGATGCACTTCGAACTTTCATAAGCAGTTCAATGCCCTTTGGAACGCTGATGGATTTGAGCTTCCCCAATATCATTTCCATTAAGGCTAATGGTTTTTCGTCATCAGATCTTCCTCCTTCAGACCTGAAGCAACCAGCATGGAAGCCTTACCTATACAAAGGAAGGCAGAGAATTCTATTTTCTATTCATGAGACTGTCCATGCTGCTATGTACGACCGAGATGAGATTCCAGATAGTATATTAATTTCTGGACATATAAATTGCCGAGCAGAGTTAGAAGGATTGCCTGATGTGTCCTTCCCCTTGATAGGACTGAACTCAGATAAGATTGAAGTTTTATCATTTCATCCTTGTGCTCAAGTTCCAGAACAAGGTGTTGATAAGCAGGCTGTGATGTTTTCACCACCATTAGGTAATTTTGCTTTAATGCGTTACCAAGCAGTCTGTGGTATTGGACCACCCATAAAGGGATTTTACCAATTGTCTATGGTATCTGAGGATAAAGGTGATTTTTTATTCAAATTACGGCTAATGGATGGTTACAAGTCTCCTCTCACAATGGAGTTTTGTACTGTGACTATGCCTTTTCCAAGGAGACGGGTTGTATCTTTTGATGGGACTCCCTCGGTCGGAATAGTTTCAACTACAGAGCATTCTGTTGAATGGAAGATTATAATGGGCGGGCGGGGTCTTAGTGGGAAAAGTATCGAGGCAACTTTTCCTGGTAAAGTCCAGTTTGCACCATGGAAACCCCAAATATTACCTCCGTCTAGTTTGGCCTTTGGAATCATAACTGATGAAGATAGTGATATTGAGACGGATGGTAATAATAATAGTATGGTCAATGTAGAGGAGTTCTTAATGGAGAAAATGAGCAAGGATCTTCATCCTGCCGATCTGGAGGAGCCATTTTGCTGGCATGCATACAACTATGCCAAGGTGTCCTTCAAAATTGTTGGAGCTTCATTGTCTGGAATGTCAATTGATCCGAAATCTGTAAGCATTTATCCAGCTGTGAAAGCACCAGTGGAGTCCTCTACTCAGGTTACATCTGGGGATTACATATTGTGGAATACATTGGGTAGATGCCCATCTGCTGTAGCAGCTACAAAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adap_comp_sub

Protein Analysis

454

Amino Acids

48.88

Weight (kDa)

5.36

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 128 - 427 1.2e-29 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 920
AatII GACGTC 1 cut(s) 42
AccB7I CCANNNNNTGG 1 cut(s) 80
AciI CCGC 3 cut(s) 5, 927, 931
AclWI GGATC 2 cut(s) 1134, 1226
AcsI RAATTY 1 cut(s) 417
AcuI CTGAAG 2 cut(s) 348, 392
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 458, 817
AfiI CCNNNNNNNGG 6 cut(s) 80, 554, 617, 656, 713, 875
AgsI TTSAA 6 cut(s) 276, 578, 766, 888, 908, 1195
AjnI CCWGG 1 cut(s) 967
AjuI GAANNNNNNNTTGG 2 cut(s) 1175, 1207
AluBI AGCT 4 cut(s) 306, 1208, 1259, 1352
AluI AGCT 4 cut(s) 306, 1208, 1259, 1352
Alw21I GWGCWC 1 cut(s) 604
Alw26I GTCTC 4 cut(s) 431, 798, 835, 1055
AlwI GGATC 2 cut(s) 1134, 1226
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 2 cut(s) 233, 1023
ApeKI GCWGC 4 cut(s) 108, 152, 449, 1349
ApoI RAATTY 1 cut(s) 417
AseI ATTAAT 1 cut(s) 488
Asp700I GAANNNNTTC 1 cut(s) 883
AspS9I GGNCC 1 cut(s) 701
AsuHPI GGTGA 3 cut(s) 32, 639, 764
AsuII TTCGAA 1 cut(s) 256
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 701
AxyI CCTNAGG 1 cut(s) 51
BanII GRGCYC 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 604
BbvI GCAGC 4 cut(s) 120, 139, 436, 1361
BccI CCATC 6 cut(s) 82, 289, 776, 854, 1061, 1345
BceAI ACGGC 1 cut(s) 788
BciT130I CCWGG 1 cut(s) 969
BcoDI GTCTC 4 cut(s) 431, 798, 835, 1055
BfaI CTAG 3 cut(s) 180, 231, 1017
BfmI CTRYAG 2 cut(s) 891, 1344
BglII AGATCT 1 cut(s) 352
BisI GCNGC 5 cut(s) 6, 109, 153, 450, 1350
BlsI GCNGC 5 cut(s) 7, 110, 154, 451, 1351
Bme1390I CCNGG 1 cut(s) 969
Bme18I GGWCC 1 cut(s) 701
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 701
BmiI GGNNCC 2 cut(s) 48, 1152
BmrFI CCNGG 1 cut(s) 969
BmsI GCATC 5 cut(s) 77, 173, 202, 238, 1322
BpmI CTGGAG 1 cut(s) 1166
Bpu14I TTCGAA 1 cut(s) 256
BpuEI CTTGAG 2 cut(s) 118, 588
BsaBI GATNNNNATC 2 cut(s) 1131, 1336
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 836, 870, 989, 1182
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 554, 617, 656, 713, 875
Bse1I ACTGG 7 cut(s) 37, 109, 158, 199, 229, 979, 1271
Bse21I CCTNAGG 1 cut(s) 51
Bse8I GATNNNNATC 2 cut(s) 1131, 1336
BseBI CCWGG 1 cut(s) 969
BseDI CCNNGG 4 cut(s) 836, 870, 989, 1182
BseGI GGATG 5 cut(s) 164, 592, 787, 1072, 1132
BseJI GATNNNNATC 2 cut(s) 1131, 1336
BseLI CCNNNNNNNGG 6 cut(s) 80, 554, 617, 656, 713, 875
BseMII CTCAG 4 cut(s) 42, 579, 732, 1301
BseNI ACTGG 7 cut(s) 37, 109, 158, 199, 229, 979, 1271
BseRI GAGGAG 3 cut(s) 786, 1112, 1163
BseXI GCAGC 4 cut(s) 120, 139, 436, 1361
BsgI GTGCAG 1 cut(s) 138
Bsh1285I CGRYCG 2 cut(s) 463, 876
BshFI GGCC 2 cut(s) 235, 1025
BsiEI CGRYCG 2 cut(s) 463, 876
BsiHKAI GWGCWC 1 cut(s) 604
BsiHKCI CYCGRG 1 cut(s) 169
BslFI GGGAC 1 cut(s) 877
BslI CCNNNNNNNGG 6 cut(s) 80, 554, 617, 656, 713, 875
BsmAI GTCTC 4 cut(s) 431, 798, 835, 1055
BsmBI CGTCTC 2 cut(s) 835, 1055
BsmFI GGGAC 1 cut(s) 877
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 2 cut(s) 235, 1025
BsoBI CYCGRG 1 cut(s) 169
Bsp119I TTCGAA 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 176, 604
Bsp143I GATC 4 cut(s) 352, 1126, 1141, 1231
Bsp19I CCATGG 1 cut(s) 989
BspACI CCGC 3 cut(s) 5, 927, 931
BspANI GGCC 2 cut(s) 235, 1025
BspCNI CTCAG 4 cut(s) 43, 578, 733, 1300
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 2 cut(s) 48, 1152
BspPI GGATC 2 cut(s) 1134, 1226
BspT104I TTCGAA 1 cut(s) 256
BsrI ACTGG 7 cut(s) 37, 109, 158, 199, 229, 979, 1271
BssECI CCNNGG 4 cut(s) 836, 870, 989, 1182
BssMI GATC 4 cut(s) 352, 1126, 1141, 1231
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 3 cut(s) 836, 989, 1182
Bst2UI CCWGG 1 cut(s) 969
Bst4CI ACNGT 3 cut(s) 202, 442, 820
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 4 cut(s) 633, 929, 1163, 1167
BstDEI CTNAG 5 cut(s) 51, 565, 741, 938, 1287
BstDSI CCRYGG 1 cut(s) 989
BstF5I GGATG 5 cut(s) 164, 592, 787, 1072, 1132
BstKTI GATC 4 cut(s) 355, 1129, 1144, 1234
BstMAI GTCTC 4 cut(s) 431, 798, 835, 1055
BstMBI GATC 4 cut(s) 352, 1126, 1141, 1231
BstMCI CGRYCG 2 cut(s) 463, 876
BstMWI GCNNNNNNNGC 5 cut(s) 11, 161, 180, 1265, 1349
BstNI CCWGG 1 cut(s) 969
BstNSI RCATGY 2 cut(s) 14, 1169
BstSCI CCNGG 1 cut(s) 967
BstSFI CTRYAG 2 cut(s) 891, 1344
BstV1I GCAGC 4 cut(s) 120, 139, 436, 1361
BstX2I RGATCY 2 cut(s) 352, 1126
BstXI CCANNNNNNTGG 1 cut(s) 1161
BstYI RGATCY 2 cut(s) 352, 1126
Bsu36I CCTNAGG 1 cut(s) 51
BsuRI GGCC 2 cut(s) 235, 1025
BtgI CCRYGG 1 cut(s) 989
BtsCI GGATG 5 cut(s) 164, 592, 787, 1072, 1132
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 190, 1278
Cac8I GCNNGC 4 cut(s) 633, 929, 1163, 1167
CciI TCATGA 1 cut(s) 433
Cfr13I GGNCC 1 cut(s) 701
Csp6I GTAC 2 cut(s) 457, 816
CviAII CATG 6 cut(s) 11, 384, 434, 446, 990, 1166
CviQI GTAC 2 cut(s) 457, 816
DdeI CTNAG 5 cut(s) 51, 565, 741, 938, 1287
DpnI GATC 4 cut(s) 354, 1128, 1143, 1233
DpnII GATC 4 cut(s) 352, 1126, 1141, 1231
Eco130I CCWWGG 3 cut(s) 836, 989, 1182
Eco147I AGGCCT 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 701
Eco57I CTGAAG 2 cut(s) 348, 392
Eco81I CCTNAGG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 169
EcoRI GAATTC 1 cut(s) 417
EcoRII CCWGG 1 cut(s) 967
EcoT14I CCWWGG 3 cut(s) 836, 989, 1182
EcoT22I ATGCAT 2 cut(s) 70, 1171
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 3 cut(s) 836, 989, 1182
Esp3I CGTCTC 2 cut(s) 835, 1055
FaeI CATG 6 cut(s) 14, 387, 437, 449, 993, 1169
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 1175, 1207
FaqI GGGAC 1 cut(s) 877
FatI CATG 6 cut(s) 10, 383, 433, 445, 989, 1165
FauI CCCGC 2 cut(s) 920, 924
Fnu4HI GCNGC 5 cut(s) 6, 109, 153, 450, 1350
FokI GGATG 5 cut(s) 151, 579, 794, 1079, 1119
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 5 cut(s) 6, 109, 153, 450, 1350
FspBI CTAG 3 cut(s) 180, 231, 1017
GluI GCNGC 5 cut(s) 6, 109, 153, 450, 1350
GsuI CTGGAG 1 cut(s) 1166
HaeIII GGCC 2 cut(s) 235, 1025
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 14, 387, 437, 449, 993, 1169
HinfI GANTC 5 cut(s) 95, 472, 865, 1032, 1277
HphI GGTGA 3 cut(s) 32, 639, 764
Hpy188I TCNGA 7 cut(s) 211, 352, 367, 568, 742, 878, 1236
Hpy188III TCNNGA 6 cut(s) 434, 476, 495, 611, 1145, 1218
Hpy99I CGWCG 2 cut(s) 41, 44
HpyAV CCTTC 5 cut(s) 372, 402, 518, 553, 1201
HpyCH4III ACNGT 3 cut(s) 202, 442, 820
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 7 cut(s) 14, 68, 155, 164, 251, 986, 1169
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 161, 180, 1265, 1349
HpyF3I CTNAG 5 cut(s) 51, 565, 741, 938, 1287
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 14, 387, 437, 449, 993, 1169
Kzo9I GATC 4 cut(s) 352, 1126, 1141, 1231
LmnI GCTCC 2 cut(s) 1150, 1205
Lsp1109I GCAGC 4 cut(s) 120, 139, 436, 1361
LweI GCATC 5 cut(s) 77, 173, 202, 238, 1322
MaeI CTAG 3 cut(s) 180, 231, 1017
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 7 cut(s) 20, 190, 202, 677, 785, 820, 1291
MalI GATC 4 cut(s) 354, 1128, 1143, 1233
MboI GATC 4 cut(s) 352, 1126, 1141, 1231
MboII GAAGA 4 cut(s) 347, 925, 1058, 1121
MfeI CAATTG 4 cut(s) 15, 147, 725, 1227
MflI RGATCY 2 cut(s) 352, 1126
MhlI GDGCHC 2 cut(s) 176, 604
MlyI GAGTC 2 cut(s) 859, 1286
MmeI TCCRAC 3 cut(s) 61, 856, 1183
Mph1103I ATGCAT 2 cut(s) 70, 1171
MroXI GAANNNNTTC 1 cut(s) 883
MseI TTAA 4 cut(s) 327, 488, 671, 1106
MslI CAYNNNNRTG 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 1259
MspR9I CCNGG 1 cut(s) 969
MunI CAATTG 4 cut(s) 15, 147, 725, 1227
Mva1269I GAATGC 1 cut(s) 898
MvaI CCWGG 1 cut(s) 969
MwoI GCNNNNNNNGC 5 cut(s) 11, 161, 180, 1265, 1349
NcoI CCATGG 1 cut(s) 989
NdeII GATC 4 cut(s) 352, 1126, 1141, 1231
NlaIII CATG 6 cut(s) 14, 387, 437, 449, 993, 1169
NlaIV GGNNCC 2 cut(s) 48, 1152
NmeAIII GCCGAG 1 cut(s) 536
NmuCI GTSAC 4 cut(s) 20, 190, 202, 820
NsiI ATGCAT 2 cut(s) 70, 1171
NspI RCATGY 2 cut(s) 14, 1169
NspV TTCGAA 1 cut(s) 256
PaeI GCATGC 1 cut(s) 1169
PaeR7I CTCGAG 1 cut(s) 169
PagI TCATGA 1 cut(s) 433
PceI AGGCCT 1 cut(s) 235
PctI GAATGC 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 883
PfeI GAWTC 3 cut(s) 95, 472, 1032
PflMI CCANNNNNTGG 1 cut(s) 80
PkrI GCNGC 5 cut(s) 7, 110, 154, 451, 1351
PleI GAGTC 2 cut(s) 859, 1285
PpsI GAGTC 2 cut(s) 859, 1285
PshBI ATTAAT 1 cut(s) 488
PsiI TTATAA 1 cut(s) 920
Psp6I CCWGG 1 cut(s) 967
PspGI CCWGG 1 cut(s) 967
PspN4I GGNNCC 2 cut(s) 48, 1152
PspPI GGNCC 1 cut(s) 701
PspXI VCTCGAGB 1 cut(s) 169
PsuI RGATCY 2 cut(s) 352, 1126
PvuII CAGCTG 1 cut(s) 1259
RsaI GTAC 2 cut(s) 458, 817
RsaNI GTAC 2 cut(s) 457, 816
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 4 cut(s) 327, 488, 671, 1106
SatI GCNGC 5 cut(s) 6, 109, 153, 450, 1350
Sau3AI GATC 4 cut(s) 352, 1126, 1141, 1231
Sau96I GGNCC 1 cut(s) 701
SchI GAGTC 2 cut(s) 859, 1286
ScrFI CCNGG 1 cut(s) 969
SduI GDGCHC 2 cut(s) 176, 604
SfaNI GCATC 5 cut(s) 77, 173, 202, 238, 1322
SfcI CTRYAG 2 cut(s) 891, 1344
Sfr274I CTCGAG 1 cut(s) 169
SfuI TTCGAA 1 cut(s) 256
SinI GGWCC 1 cut(s) 701
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 3 cut(s) 133, 169, 603
SmoI CTYRAG 3 cut(s) 133, 169, 603
SphI GCATGC 1 cut(s) 1169
SseBI AGGCCT 1 cut(s) 235
SsiI CCGC 3 cut(s) 5, 927, 931
SspI AATATT 1 cut(s) 1005
SspMI CTAG 3 cut(s) 180, 231, 1017
StuI AGGCCT 1 cut(s) 235
StyD4I CCNGG 1 cut(s) 967
StyI CCWWGG 3 cut(s) 836, 989, 1182
TaaI ACNGT 3 cut(s) 202, 442, 820
TaiI ACGT 1 cut(s) 42
TaqI TCGA 4 cut(s) 122, 170, 256, 954
TaqII GACCGA 2 cut(s) 477, 862
TatI WGTACW 1 cut(s) 815
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 95, 472, 1032
Tru1I TTAA 4 cut(s) 327, 488, 671, 1106
Tru9I TTAA 4 cut(s) 327, 488, 671, 1106
TscAI CASTG 2 cut(s) 190, 1278
TseFI GTSAC 4 cut(s) 20, 190, 202, 820
TseI GCWGC 4 cut(s) 108, 152, 449, 1349
Tsp45I GTSAC 4 cut(s) 20, 190, 202, 820
TspDTI ATGAA 6 cut(s) 253, 422, 581, 1059, 1121, 1200
TspGWI ACGGA 2 cut(s) 1002, 1079
TspRI CASTG 2 cut(s) 190, 1278
Van91I CCANNNNNTGG 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 701
VspI ATTAAT 1 cut(s) 488
XapI RAATTY 1 cut(s) 417
XceI RCATGY 2 cut(s) 14, 1169
XcmI CCANNNNNNNNNTGG 1 cut(s) 689
XhoI CTCGAG 1 cut(s) 169
XmnI GAANNNNTTC 1 cut(s) 883
XspI CTAG 3 cut(s) 180, 231, 1017
ZraI GACGTC 1 cut(s) 40
Zsp2I ATGCAT 2 cut(s) 70, 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.