Rh4AG260500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0018964
Physical Location & Seq
Reverse (-)
57674572 .. 57675354
783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG260500.1

Sequence Viewer

Length: 579 bp
ATGGCCACCCAACTCTATTTCTCTCTCTTACTCCGGCACATCCCGATTGAATCAGAAATCTTCAAAATTCCGGCAACAATTGTAGCTATTTTCTTCCTTGCAGCAGAGTCCTTCACTCCCCCAACAGACAATCTTCTCCCTGGAGAAATCCGCCACCAGAGGCTCCTGCGAACCGACGAAGGGGTGGCGGCCAGCGATGGGGTTGATTCCGGCGATGCGCGGCACCGGAGAGTCGAAATCGAAGCGGTCGATGTAGACGAACTGGCCGAGCTGGAGGCGGTTGGCGAGGATGAGATCGGTGTCTTTCTCGGAGAGGGAGACGTAGGTGGAGTTGAGGGAGTCGGAGAGCTGGACGTAGAAGCCTTGGTTGGGCCAGAGGTCGTCGGAGTCGACGAGGGCGGGGACTATGCCGATGACTTGGAGCAGGGCAGAGTGGTGGTCGCCGGTGACTTTGATGGCGGAGTTCATGGTCTGGAGGAGCCGGGAGTGGGTGACGCCATTGGGGGTGAGAGAATCAGAGAGAGAGAGAGAGGATTGGGATTTGGAATGTGGAGGACATGCGGTGGTCGCCGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

20.51

Weight (kDa)

4.26

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018964)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0114801 RchiOBHm_Chr2g0114851
rosa_laevigata RLG00000018123
rosa_roxburghii Rroxscaffold_2G00130340 Rroxscaffold_7G00169110 Rroxscaffold_7G00185000
rosa_rugosa Rorug06G0159800
rosa_samantha Rh3AG322600 Rh4AG260500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 222
AccI GTMKAC 2 cut(s) 255, 390
AccII CGCG 1 cut(s) 220
AciI CCGC 8 cut(s) 151, 188, 220, 245, 278, 399, 459, 561
AcoI YGGCCR 3 cut(s) 3, 189, 264
AcsI RAATTY 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 495
AfiI CCNNNNNNNGG 4 cut(s) 180, 198, 369, 488
AgsI TTSAA 2 cut(s) 50, 64
AjnI CCWGG 1 cut(s) 139
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AluBI AGCT 3 cut(s) 86, 271, 349
AluI AGCT 3 cut(s) 86, 271, 349
Alw26I GTCTC 1 cut(s) 312
AoxI GGCC 4 cut(s) 3, 189, 264, 371
ApeKI GCWGC 1 cut(s) 101
ApoI RAATTY 1 cut(s) 66
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 2 cut(s) 483, 572
AsuHPI GGTGA 3 cut(s) 458, 503, 518
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 113
BccI CCATC 2 cut(s) 191, 449
BciT130I CCWGG 1 cut(s) 141
BcnI CCSGG 2 cut(s) 483, 572
BcoDI GTCTC 1 cut(s) 312
BisI GCNGC 3 cut(s) 102, 189, 221
BlsI GCNGC 3 cut(s) 103, 190, 222
Bme1390I CCNGG 3 cut(s) 141, 483, 572
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 3 cut(s) 164, 224, 480
BmrFI CCNGG 3 cut(s) 141, 483, 572
BmsI GCATC 1 cut(s) 205
BpmI CTGGAG 3 cut(s) 162, 293, 494
BpuMI CCSGG 2 cut(s) 483, 572
BsaHI GRCGYC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 139, 363
BsaWI WCCGGW 1 cut(s) 225
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 198, 369, 488
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 1 cut(s) 267
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 139, 363
BseGI GGATG 2 cut(s) 39, 295
BseLI CCNNNNNNNGG 4 cut(s) 180, 198, 369, 488
BseNI ACTGG 1 cut(s) 267
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 1 cut(s) 113
Bsh1236I CGCG 1 cut(s) 220
Bsh1285I CGRYCG 1 cut(s) 249
BshFI GGCC 4 cut(s) 5, 191, 266, 373
BshNI GGYRCC 1 cut(s) 222
BsiEI CGRYCG 1 cut(s) 249
BsiSI CCGG 7 cut(s) 34, 71, 210, 226, 444, 482, 571
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 4 cut(s) 180, 198, 369, 488
BsmAI GTCTC 1 cut(s) 312
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 416
BsnI GGCC 4 cut(s) 5, 191, 266, 373
Bsp143I GATC 1 cut(s) 294
BspACI CCGC 8 cut(s) 151, 188, 220, 245, 278, 399, 459, 561
BspANI GGCC 4 cut(s) 5, 191, 266, 373
BspFNI CGCG 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 164, 224, 480
BspT107I GGYRCC 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 1 cut(s) 267
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 2 cut(s) 139, 363
BssMI GATC 1 cut(s) 294
BssNI GRCGYC 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 141
BstACI GRCGYC 1 cut(s) 495
BstC8I GCNNGC 1 cut(s) 193
BstF5I GGATG 2 cut(s) 39, 295
BstFNI CGCG 1 cut(s) 220
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 1 cut(s) 294
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 1 cut(s) 567
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 3 cut(s) 139, 481, 570
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 113
BsuRI GGCC 4 cut(s) 5, 191, 266, 373
BtgZI GCGATG 2 cut(s) 210, 228
BtsCI GGATG 2 cut(s) 39, 295
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 1 cut(s) 503
CviAII CATG 2 cut(s) 467, 558
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
EaeI YGGCCR 3 cut(s) 3, 189, 264
EciI GGCGGA 2 cut(s) 140, 474
Eco130I CCWWGG 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 363
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 2 cut(s) 470, 561
FaiI YATR 3 cut(s) 408, 468, 559
FaqI GGGAC 1 cut(s) 416
FatI CATG 2 cut(s) 466, 557
FauI CCCGC 1 cut(s) 392
FblI GTMKAC 2 cut(s) 255, 390
Fnu4HI GCNGC 3 cut(s) 102, 189, 221
FokI GGATG 2 cut(s) 26, 302
Fsp4HI GCNGC 3 cut(s) 102, 189, 221
GlaI GCGC 1 cut(s) 219
GluI GCNGC 3 cut(s) 102, 189, 221
GsuI CTGGAG 3 cut(s) 162, 293, 494
HaeIII GGCC 4 cut(s) 5, 191, 266, 373
HapII CCGG 7 cut(s) 34, 71, 210, 226, 444, 482, 571
HgaI GACGC 1 cut(s) 503
HhaI GCGC 1 cut(s) 220
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 2 cut(s) 470, 561
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 7 cut(s) 50, 107, 206, 231, 339, 387, 513
HpaII CCGG 7 cut(s) 34, 71, 210, 226, 444, 482, 571
HphI GGTGA 3 cut(s) 458, 503, 518
Hpy166II GTNNAC 2 cut(s) 256, 391
Hpy188I TCNGA 5 cut(s) 55, 311, 344, 386, 518
Hpy188III TCNNGA 2 cut(s) 43, 473
Hpy8I GTNNAC 2 cut(s) 256, 391
Hpy99I CGWCG 3 cut(s) 179, 386, 395
HpyAV CCTTC 2 cut(s) 121, 173
HpyCH4IV ACGT 2 cut(s) 321, 354
HpyCH4V TGCA 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 1 cut(s) 567
HpySE526I ACGT 2 cut(s) 321, 354
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 2 cut(s) 470, 561
HspAI GCGC 1 cut(s) 218
Kzo9I GATC 1 cut(s) 294
LmnI GCTCC 3 cut(s) 168, 421, 478
Lsp1109I GCAGC 1 cut(s) 113
LweI GCATC 1 cut(s) 205
MaeII ACGT 2 cut(s) 321, 354
MaeIII GTNAC 2 cut(s) 446, 491
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 3 cut(s) 52, 85, 125
MfeI CAATTG 1 cut(s) 78
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 66, 78
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 116, 240, 348, 396
MmeI TCCRAC 2 cut(s) 322, 364
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 7 cut(s) 34, 71, 210, 226, 444, 482, 571
MspR9I CCNGG 3 cut(s) 141, 483, 572
MunI CAATTG 1 cut(s) 78
MvaI CCWGG 1 cut(s) 141
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 1 cut(s) 567
NciI CCSGG 2 cut(s) 483, 572
NdeII GATC 1 cut(s) 294
NlaIII CATG 2 cut(s) 470, 561
NlaIV GGNNCC 3 cut(s) 164, 224, 480
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 2 cut(s) 446, 491
NspI RCATGY 1 cut(s) 561
PcsI WCGNNNNNNNCGW 5 cut(s) 246, 255, 264, 387, 390
PfeI GAWTC 3 cut(s) 50, 206, 513
PkrI GCNGC 3 cut(s) 103, 190, 222
PleI GAGTC 4 cut(s) 115, 239, 347, 395
PpsI GAGTC 4 cut(s) 115, 239, 347, 395
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 3 cut(s) 164, 224, 480
PspPI GGNCC 1 cut(s) 371
SalI GTCGAC 1 cut(s) 389
SatI GCNGC 3 cut(s) 102, 189, 221
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 1 cut(s) 371
SchI GAGTC 4 cut(s) 116, 240, 348, 396
ScrFI CCNGG 3 cut(s) 141, 483, 572
SetI ASST 7 cut(s) 88, 273, 324, 328, 351, 357, 381
SfaNI GCATC 1 cut(s) 205
SgrAI CRCCGGYG 1 cut(s) 443
Sse9I AATT 2 cut(s) 66, 78
SsiI CCGC 8 cut(s) 151, 188, 220, 245, 278, 399, 459, 561
StyD4I CCNGG 3 cut(s) 139, 481, 570
StyI CCWWGG 1 cut(s) 363
TaiI ACGT 2 cut(s) 324, 357
TaqI TCGA 4 cut(s) 234, 240, 249, 390
TasI AATT 2 cut(s) 66, 78
TauI GCSGC 2 cut(s) 191, 223
TfiI GAWTC 3 cut(s) 50, 206, 513
TseFI GTSAC 2 cut(s) 446, 491
TseI GCWGC 1 cut(s) 101
Tsp45I GTSAC 2 cut(s) 446, 491
TspDTI ATGAA 1 cut(s) 455
XapI RAATTY 1 cut(s) 66
XceI RCATGY 1 cut(s) 561
XmiI GTMKAC 2 cut(s) 255, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.