Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0018597

Physical Location & Seq

Genomic Coordinates Reverse (-)

59401137 .. 59402270

Gene Structure 1134 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG277500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGGCATCGTCTCTCTCATTCAAACAAGTCCTGGGCCCTTCAAGGACTGAAGGGCTTTCTGGGTTGTGCTCTTGTCAGTCTGATCTTCGAAACCTGTCGTCCCCCTCCGTACAATTCTTGGTCCGCCCTCGAACCTTCAAGAAACTCAAAGTGCTGGCAGCTGGGAATACTTCAGGAAACTACTTTCGGGTTACAACTTATGGAGAGTTTCGTCGTGCTGGTGTTAGTTTTGTAATGGATGGAGTTCCTCCTCGGAGTCAACTATCTGAAGCTGATTTGCAAGGGGACCTCGACAGAATAAGGAGGCCAGGTCAGATCAGCCCACGTATTAACCCTGGAAACAAAACTGACACATGCAAGATACTCTCGGGAGTCCATGAAGGATTGACTATTGGGACACCAATTCATGTGTTTATACCCAATCCTGATCAGACAGGTCGATGTGGTTATGACGAAATCTCCGTAGCTTATAGGCCTTCACATGCAGATCTAACACGCGACATGAAGTATGGTATCAGATCAATACTTAAGGGTGGTGGTATATTTTCAGCTAGGGAAACAATAGGAAGAGTTGCTGCAGGGGCAGCTGCGAAGAAAATCCTCAAGGACTTGGCCGGAATCGAGGTTCTTGGTTATGTTTCACAAGTTCACAAGGTTGTCCTCCCGGAGGACTTGGTTGATCATGACACTCTGACACTTGATCAGATAGAGAGCAATAGAGTTAGGTGTCCAGATCATGAATACGCAGAGAAGATGATAGCTGCCATCCATGCTGTTAGGGTTAGAGGAGAGGATCAACCTATTGGTGGTGTTGTCAGGTGCATAGTTAGGAATTGCCCGCGGGGTCTTCTTGGTTCGCCTTTCTTTGACAAACTTGGAGCTGAATTTGCTAAAGCTTGTCTGTCATTACCTGCAACAAAGGGATTTGAATTCGGAAGGGGCCTTGCAGCAGGTACCTTAAACATGAAGATAGCTTTCGAGCCAACAGCTACAAATGCAAGGAACCAGAACGCAGTGACTACAGGTAAGGAGAGTGAACTAATGGATACTTGTGTTGTCCCCCGAGCGGTTCCAATGGTGGAGGCTCAAGTGGCGCTGGTGCTTATGGATGAATTAATGACTCAATATGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004107 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010181 GO:0016053 GO:0016829 GO:0016835 GO:0016838 GO:0019438 GO:0019752 GO:0031974 GO:0031981 GO:0032553 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0048037 GO:0050662 GO:0070013 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

Chorismate_synt

Protein Analysis

377

Amino Acids

40.82

Weight (kDa)

8.91

Isoelectric Point (pI)

35.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Chorismate_synt	PF01264	62 - 317	5.4e-68	Chorismate synthase
Chorismate_synt	PF01264	320 - 373	2.4e-06	Chorismate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0018597)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr4g0427911
rosa_laevigata	RLG00000007201
rosa_multiflora	Rmu_sc0000066.1_g000014 Rmu_sc0009221.1_g000002
rosa_rugosa	Rorug04G0220500
rosa_samantha	Rh4AG277500 Rh4BG283300 Rh4CG298800 Rh4DG280400
rosa_wichuraiana	Rw4G024110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	919, 941
Acc65I	GGTACC	1 cut(s)	953
AccB1I	GGYRCC	1 cut(s)	953
AccBSI	CCGCTC	1 cut(s)	1067
AccII	CGCG	2 cut(s)	498, 841
AciI	CCGC	4 cut(s)	124, 839, 841, 1067
AclWI	GGATC	1 cut(s)	801
AcoI	YGGCCR	1 cut(s)	612
AcsI	RAATTY	2 cut(s)	884, 929
AcuI	CTGAAG	3 cut(s)	69, 156, 288
AfaI	GTAC	2 cut(s)	111, 955
AfiI	CCNNNNNNNGG	3 cut(s)	667, 806, 1066
AflII	CTTAAG	1 cut(s)	527
AgsI	TTSAA	4 cut(s)	22, 42, 139, 929
AjnI	CCWGG	3 cut(s)	30, 307, 334
AloI	GAACNNNNNNTCC	2 cut(s)	609, 641
Alw21I	GWGCWC	1 cut(s)	71
Alw26I	GTCTC	1 cut(s)	15
AlwI	GGATC	1 cut(s)	801
Ama87I	CYCGRG	2 cut(s)	367, 1062
AoxI	GGCC	5 cut(s)	34, 305, 473, 612, 940
ApaI	GGGCCC	1 cut(s)	38
ApeKI	GCWGC	6 cut(s)	158, 575, 584, 587, 761, 947
ApoI	RAATTY	2 cut(s)	884, 929
AseI	ATTAAT	1 cut(s)	1115
Asp718I	GGTACC	1 cut(s)	953
AspLEI	GCGC	1 cut(s)	1096
AspS9I	GGNCC	5 cut(s)	34, 35, 121, 286, 940
AsuC2I	CCSGG	1 cut(s)	665
AsuII	TTCGAA	1 cut(s)	88
AvaI	CYCGRG	2 cut(s)	367, 1062
AvaII	GGWCC	2 cut(s)	121, 286
BaeGI	GKGCMC	1 cut(s)	38
BaeI	ACNNNNGTAYC	2 cut(s)	1038, 1071
BanI	GGYRCC	1 cut(s)	953
BanII	GRGCYC	1 cut(s)	38
BbsI	GAAGAC	1 cut(s)	839
Bbv12I	GWGCWC	1 cut(s)	71
BbvI	GCAGC	6 cut(s)	170, 562, 574, 596, 748, 959
BccI	CCATC	2 cut(s)	233, 773
BciT130I	CCWGG	3 cut(s)	32, 309, 336
BciVI	GTATCC	1 cut(s)	1039
BclI	TGATCA	3 cut(s)	427, 679, 700
BcnI	CCSGG	1 cut(s)	665
BcoDI	GTCTC	1 cut(s)	15
BfaI	CTAG	1 cut(s)	552
BfmI	CTRYAG	2 cut(s)	576, 1020
BfoI	RGCGCY	1 cut(s)	1097
BfrI	CTTAAG	1 cut(s)	527
BfuAI	ACCTGC	2 cut(s)	919, 941
BfuI	GTATCC	1 cut(s)	1039
BglII	AGATCT	1 cut(s)	487
BisI	GCNGC	6 cut(s)	159, 576, 585, 588, 762, 948
BlsI	GCNGC	6 cut(s)	160, 577, 586, 589, 763, 949
Bme1390I	CCNGG	4 cut(s)	32, 309, 336, 665
Bme18I	GGWCC	2 cut(s)	121, 286
BmeT110I	CYCGRG	2 cut(s)	367, 1062
BmgT120I	GGNCC	5 cut(s)	34, 35, 121, 286, 940
BmiI	GGNNCC	6 cut(s)	36, 287, 941, 955, 1004, 1071
BmrFI	CCNGG	4 cut(s)	32, 309, 336, 665
BmsI	GCATC	1 cut(s)	14
BpiI	GAAGAC	1 cut(s)	839
Bpu14I	TTCGAA	1 cut(s)	88
BpuEI	CTTGAG	2 cut(s)	587, 1071
BpuMI	CCSGG	1 cut(s)	665
BsaAI	YACGTR	1 cut(s)	326
BsaJI	CCNNGG	4 cut(s)	31, 251, 334, 839
BsaXI	ACNNNNNCTCC	4 cut(s)	295, 325, 443, 473
Bsc4I	CCNNNNNNNGG	3 cut(s)	667, 806, 1066
BseBI	CCWGG	3 cut(s)	32, 309, 336
BseDI	CCNNGG	4 cut(s)	31, 251, 334, 839
BseGI	GGATG	3 cut(s)	244, 765, 1114
BseLI	CCNNNNNNNGG	3 cut(s)	667, 806, 1066
BseRI	GAGGAG	2 cut(s)	240, 801
BseSI	GKGCMC	1 cut(s)	38
BseXI	GCAGC	6 cut(s)	170, 562, 574, 596, 748, 959
BseYI	CCCAGC	1 cut(s)	161
Bsh1236I	CGCG	2 cut(s)	498, 841
BshFI	GGCC	5 cut(s)	36, 307, 475, 614, 942
BshNI	GGYRCC	1 cut(s)	953
BsiHKAI	GWGCWC	1 cut(s)	71
BsiHKCI	CYCGRG	2 cut(s)	367, 1062
BsiSI	CCGG	2 cut(s)	615, 665
BslFI	GGGAC	4 cut(s)	85, 299, 409, 1043
BslI	CCNNNNNNNGG	3 cut(s)	667, 806, 1066
BsmAI	GTCTC	1 cut(s)	15
BsmBI	CGTCTC	1 cut(s)	15
BsmFI	GGGAC	4 cut(s)	85, 299, 409, 1043
BsnI	GGCC	5 cut(s)	36, 307, 475, 614, 942
BsoBI	CYCGRG	2 cut(s)	367, 1062
Bsp119I	TTCGAA	1 cut(s)	88
Bsp120I	GGGCCC	1 cut(s)	34
Bsp1286I	GDGCHC	2 cut(s)	38, 71
Bsp143I	GATC	9 cut(s)	82, 315, 427, 487, 518, 679, 700, 733, 793
BspACI	CCGC	4 cut(s)	124, 839, 841, 1067
BspANI	GGCC	5 cut(s)	36, 307, 475, 614, 942
BspFNI	CGCG	2 cut(s)	498, 841
BspHI	TCATGA	2 cut(s)	682, 736
BspLI	GGNNCC	6 cut(s)	36, 287, 941, 955, 1004, 1071
BspMAI	CTGCAG	1 cut(s)	580
BspMI	ACCTGC	2 cut(s)	919, 941
BspPI	GGATC	1 cut(s)	801
BspT104I	TTCGAA	1 cut(s)	88
BspT107I	GGYRCC	1 cut(s)	953
BspTI	CTTAAG	1 cut(s)	527
BsrBI	CCGCTC	1 cut(s)	1067
BssECI	CCNNGG	4 cut(s)	31, 251, 334, 839
BssMI	GATC	9 cut(s)	82, 315, 427, 487, 518, 679, 700, 733, 793
Bst2UI	CCWGG	3 cut(s)	32, 309, 336
Bst6I	CTCTTC	1 cut(s)	562
BstAFI	CTTAAG	1 cut(s)	527
BstBAI	YACGTR	1 cut(s)	326
BstBI	TTCGAA	1 cut(s)	88
BstC8I	GCNNGC	2 cut(s)	156, 839
BstDSI	CCRYGG	1 cut(s)	839
BstENI	CCTNNNNNAGG	1 cut(s)	665
BstF5I	GGATG	3 cut(s)	244, 765, 1114
BstFNI	CGCG	2 cut(s)	498, 841
BstH2I	RGCGCY	1 cut(s)	1097
BstHHI	GCGC	1 cut(s)	1096
BstKTI	GATC	9 cut(s)	85, 318, 430, 490, 521, 682, 703, 736, 796
BstMAI	GTCTC	1 cut(s)	15
BstMBI	GATC	9 cut(s)	82, 315, 427, 487, 518, 679, 700, 733, 793
BstMWI	GCNNNNNNNGC	6 cut(s)	581, 584, 770, 887, 995, 1091
BstNI	CCWGG	3 cut(s)	32, 309, 336
BstNSI	RCATGY	2 cut(s)	357, 485
BstSCI	CCNGG	4 cut(s)	30, 307, 334, 663
BstSFI	CTRYAG	2 cut(s)	576, 1020
BstSLI	GKGCMC	1 cut(s)	38
BstUI	CGCG	2 cut(s)	498, 841
BstV1I	GCAGC	6 cut(s)	170, 562, 574, 596, 748, 959
BstV2I	GAAGAC	1 cut(s)	839
BstX2I	RGATCY	1 cut(s)	487
BstYI	RGATCY	1 cut(s)	487
BsuI	GTATCC	1 cut(s)	1039
BsuRI	GGCC	5 cut(s)	36, 307, 475, 614, 942
BtgI	CCRYGG	1 cut(s)	839
BtsCI	GGATG	3 cut(s)	244, 765, 1114
BtsI	GCAGTG	1 cut(s)	1020
BtsIMutI	CAGTG	1 cut(s)	1020
BveI	ACCTGC	2 cut(s)	919, 941
Cac8I	GCNNGC	2 cut(s)	156, 839
CciI	TCATGA	2 cut(s)	682, 736
CfoI	GCGC	1 cut(s)	1096
Cfr13I	GGNCC	5 cut(s)	34, 35, 121, 286, 940
Cfr42I	CCGCGG	1 cut(s)	842
Csp6I	GTAC	2 cut(s)	110, 954
CviAII	CATG	9 cut(s)	354, 377, 407, 482, 502, 683, 737, 770, 964
CviQI	GTAC	2 cut(s)	110, 954
DpnI	GATC	9 cut(s)	84, 317, 429, 489, 520, 681, 702, 735, 795
DpnII	GATC	9 cut(s)	82, 315, 427, 487, 518, 679, 700, 733, 793
EaeI	YGGCCR	1 cut(s)	612
Eam1104I	CTCTTC	1 cut(s)	562
EarI	CTCTTC	1 cut(s)	562
EciI	GGCGGA	1 cut(s)	113
Eco147I	AGGCCT	1 cut(s)	475
Eco24I	GRGCYC	1 cut(s)	38
Eco47I	GGWCC	2 cut(s)	121, 286
Eco57I	CTGAAG	3 cut(s)	69, 156, 288
Eco88I	CYCGRG	2 cut(s)	367, 1062
EcoNI	CCTNNNNNAGG	1 cut(s)	665
EcoO109I	RGGNCCY	3 cut(s)	35, 286, 940
EcoRI	GAATTC	1 cut(s)	929
EcoRII	CCWGG	3 cut(s)	30, 307, 334
EcoT38I	GRGCYC	1 cut(s)	38
Esp3I	CGTCTC	1 cut(s)	15
FaeI	CATG	9 cut(s)	357, 380, 410, 485, 505, 686, 740, 773, 967
FaqI	GGGAC	4 cut(s)	85, 299, 409, 1043
FatI	CATG	9 cut(s)	353, 376, 406, 481, 501, 682, 736, 769, 963
FauI	CCCGC	2 cut(s)	834, 846
FbaI	TGATCA	3 cut(s)	427, 679, 700
Fnu4HI	GCNGC	6 cut(s)	159, 576, 585, 588, 762, 948
FokI	GGATG	3 cut(s)	251, 752, 1121
FriOI	GRGCYC	1 cut(s)	38
Fsp4HI	GCNGC	6 cut(s)	159, 576, 585, 588, 762, 948
FspBI	CTAG	1 cut(s)	552
GlaI	GCGC	1 cut(s)	1095
GluI	GCNGC	6 cut(s)	159, 576, 585, 588, 762, 948
GsaI	CCCAGC	1 cut(s)	165
HaeII	RGCGCY	1 cut(s)	1097
HaeIII	GGCC	5 cut(s)	36, 307, 475, 614, 942
HapII	CCGG	2 cut(s)	615, 665
HhaI	GCGC	1 cut(s)	1096
Hin1II	CATG	9 cut(s)	357, 380, 410, 485, 505, 686, 740, 773, 967
Hin6I	GCGC	1 cut(s)	1094
HinP1I	GCGC	1 cut(s)	1094
HincII	GTYRAC	1 cut(s)	260
HindII	GTYRAC	1 cut(s)	260
HindIII	AAGCTT	1 cut(s)	894
HinfI	GANTC	4 cut(s)	256, 372, 618, 1120
HpaII	CCGG	2 cut(s)	615, 665
Hpy166II	GTNNAC	3 cut(s)	260, 649, 1037
Hpy188I	TCNGA	9 cut(s)	82, 255, 268, 315, 432, 518, 693, 705, 935
Hpy188III	TCNNGA	7 cut(s)	139, 174, 369, 425, 683, 731, 737
Hpy8I	GTNNAC	3 cut(s)	260, 649, 1037
Hpy99I	CGWCG	1 cut(s)	216
HpyAV	CCTTC	6 cut(s)	44, 48, 145, 374, 486, 930
HpyCH4IV	ACGT	1 cut(s)	325
HpyCH4V	TGCA	8 cut(s)	280, 357, 485, 578, 822, 914, 947, 998
HpyF10VI	GCNNNNNNNGC	6 cut(s)	581, 584, 770, 887, 995, 1091
HpySE526I	ACGT	1 cut(s)	325
Hsp92II	CATG	9 cut(s)	357, 380, 410, 485, 505, 686, 740, 773, 967
HspAI	GCGC	1 cut(s)	1094
KpnI	GGTACC	1 cut(s)	957
Ksp22I	TGATCA	3 cut(s)	427, 679, 700
KspI	CCGCGG	1 cut(s)	842
Kzo9I	GATC	9 cut(s)	82, 315, 427, 487, 518, 679, 700, 733, 793
LmnI	GCTCC	1 cut(s)	878
Lsp1109I	GCAGC	6 cut(s)	170, 562, 574, 596, 748, 959
LweI	GCATC	1 cut(s)	14
MaeI	CTAG	1 cut(s)	552
MaeII	ACGT	1 cut(s)	325
MaeIII	GTNAC	2 cut(s)	190, 1015
MalI	GATC	9 cut(s)	84, 317, 429, 489, 520, 681, 702, 735, 795
MbiI	CCGCTC	1 cut(s)	1067
MboI	GATC	9 cut(s)	82, 315, 427, 487, 518, 679, 700, 733, 793
MboII	GAAGA	6 cut(s)	77, 579, 604, 763, 839, 979
MflI	RGATCY	1 cut(s)	487
MhlI	GDGCHC	2 cut(s)	38, 71
MluCI	AATT	6 cut(s)	113, 402, 832, 884, 929, 1112
MlyI	GAGTC	3 cut(s)	265, 381, 1114
MseI	TTAA	4 cut(s)	330, 528, 959, 1115
MspA1I	CMGCKG	3 cut(s)	161, 587, 841
MspCI	CTTAAG	1 cut(s)	527
MspI	CCGG	2 cut(s)	615, 665
MspR9I	CCNGG	4 cut(s)	32, 309, 336, 665
MvaI	CCWGG	3 cut(s)	32, 309, 336
MvnI	CGCG	2 cut(s)	498, 841
MwoI	GCNNNNNNNGC	6 cut(s)	581, 584, 770, 887, 995, 1091
NciI	CCSGG	1 cut(s)	665
NdeII	GATC	9 cut(s)	82, 315, 427, 487, 518, 679, 700, 733, 793
NlaIII	CATG	9 cut(s)	357, 380, 410, 485, 505, 686, 740, 773, 967
NlaIV	GGNNCC	6 cut(s)	36, 287, 941, 955, 1004, 1071
NmuCI	GTSAC	1 cut(s)	1015
NspI	RCATGY	2 cut(s)	357, 485
NspV	TTCGAA	1 cut(s)	88
PagI	TCATGA	2 cut(s)	682, 736
PceI	AGGCCT	1 cut(s)	475
PcsI	WCGNNNNNNNCGW	1 cut(s)	459
PfeI	GAWTC	1 cut(s)	618
PfoI	TCCNGGA	1 cut(s)	663
PkrI	GCNGC	6 cut(s)	160, 577, 586, 589, 763, 949
PleI	GAGTC	3 cut(s)	264, 380, 1114
PpsI	GAGTC	3 cut(s)	264, 380, 1114
Ppu21I	YACGTR	1 cut(s)	326
PpuMI	RGGWCCY	1 cut(s)	286
PshBI	ATTAAT	1 cut(s)	1115
Psp5II	RGGWCCY	1 cut(s)	286
Psp6I	CCWGG	3 cut(s)	30, 307, 334
PspFI	CCCAGC	1 cut(s)	161
PspGI	CCWGG	3 cut(s)	30, 307, 334
PspN4I	GGNNCC	6 cut(s)	36, 287, 941, 955, 1004, 1071
PspOMI	GGGCCC	1 cut(s)	34
PspPI	GGNCC	5 cut(s)	34, 35, 121, 286, 940
PspPPI	RGGWCCY	1 cut(s)	286
PstI	CTGCAG	1 cut(s)	580
PsuI	RGATCY	1 cut(s)	487
PvuII	CAGCTG	2 cut(s)	161, 587
RsaI	GTAC	2 cut(s)	111, 955
RsaNI	GTAC	2 cut(s)	110, 954
SacII	CCGCGG	1 cut(s)	842
SaqAI	TTAA	4 cut(s)	330, 528, 959, 1115
SatI	GCNGC	6 cut(s)	159, 576, 585, 588, 762, 948
Sau3AI	GATC	9 cut(s)	82, 315, 427, 487, 518, 679, 700, 733, 793
Sau96I	GGNCC	5 cut(s)	34, 35, 121, 286, 940
SchI	GAGTC	3 cut(s)	265, 381, 1114
ScrFI	CCNGG	4 cut(s)	32, 309, 336, 665
SduI	GDGCHC	2 cut(s)	38, 71
SfaNI	GCATC	1 cut(s)	14
SfcI	CTRYAG	2 cut(s)	576, 1020
Sfr303I	CCGCGG	1 cut(s)	842
SfuI	TTCGAA	1 cut(s)	88
SgrBI	CCGCGG	1 cut(s)	842
SinI	GGWCC	2 cut(s)	121, 286
SmlI	CTYRAG	3 cut(s)	527, 602, 1086
SmoI	CTYRAG	3 cut(s)	527, 602, 1086
Sse9I	AATT	6 cut(s)	113, 402, 832, 884, 929, 1112
SseBI	AGGCCT	1 cut(s)	475
SsiI	CCGC	4 cut(s)	124, 839, 841, 1067
SspMI	CTAG	1 cut(s)	552
StuI	AGGCCT	1 cut(s)	475
StyD4I	CCNGG	4 cut(s)	30, 307, 334, 663
TaiI	ACGT	1 cut(s)	328
TaqI	TCGA	6 cut(s)	88, 130, 291, 439, 621, 978
TasI	AATT	6 cut(s)	113, 402, 832, 884, 929, 1112
TfiI	GAWTC	1 cut(s)	618
Tru1I	TTAA	4 cut(s)	330, 528, 959, 1115
Tru9I	TTAA	4 cut(s)	330, 528, 959, 1115
TscAI	CASTG	1 cut(s)	1020
TseFI	GTSAC	1 cut(s)	1015
TseI	GCWGC	6 cut(s)	158, 575, 584, 587, 761, 947
Tsp45I	GTSAC	1 cut(s)	1015
TspDTI	ATGAA	6 cut(s)	393, 395, 518, 753, 980, 1125
TspGWI	ACGGA	2 cut(s)	97, 451
TspRI	CASTG	1 cut(s)	1020
Vha464I	CTTAAG	1 cut(s)	527
VpaK11BI	GGWCC	2 cut(s)	121, 286
VspI	ATTAAT	1 cut(s)	1115
XagI	CCTNNNNNAGG	1 cut(s)	665
XapI	RAATTY	2 cut(s)	884, 929
XceI	RCATGY	2 cut(s)	357, 485
XspI	CTAG	1 cut(s)	552

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.