Rh4AG408300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0019472
Physical Location & Seq
Reverse (-)
70865577 .. 70866288
712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG408300.1

Sequence Viewer

Length: 537 bp
ATGAACACAGATCCTATGCTCCAAGTCATACACCGTGTATGCTTTGATTTACTAGAAACCAAGAGATGTATTTGCCCAGTGGACGAACCAAATCTAACCCCCCCTGGCACCATCCAGCTCTTCCCTACTCAACCCCAGCCCCTACGGTGTTCGACGACCGAGCAGTGGCTGGTGCTCTCCTCCGGGTCAGCACCGCAGCGTTACAGTGCTCCATCTCCTCCGCCGCTCGCGGAGCAGTGGATCGTGCCTCTCCCGGAGAAGGTCAAGAAGGGCCGCTGCGCTTTTCTTCCCCACGGCGGCAATGAGTGGCCTCACCACCACCTCCGCACCGCCGCGATTACTCGCAATCCCAGCACGACACCGTTTCACCGACAATTCATGGACTTCCATCGCCATCCCCAAACCTCATCTCAGAGCTCCACGAATCATTTGCATGGCGGTCAGTCCATTAAGCAATTCGTAACTTCCTTATTAAGTTTTTGCTTTAGTTTCCATGGAAATTGTTTACCGAGAAACGAACTGAGGTTACCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

20.17

Weight (kDa)

8.79

Isoelectric Point (pI)

54.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019472)

Species Orthologous Gene IDs
rosa_laevigata RLG00000014089
rosa_multiflora Rmu_sc0001556.1_g000022 Rmu_sc0015522.1_g000002
rosa_roxburghii Rroxscaffold_1G00074430 Rroxscaffold_4G00322990
rosa_samantha Rh4AG408300 Rh4DG027300 Rh7CG279400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 107
AccBSI CCGCTC 1 cut(s) 226
AccII CGCG 2 cut(s) 230, 335
AclWI GGATC 2 cut(s) 5, 248
AfiI CCNNNNNNNGG 3 cut(s) 165, 259, 296
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 2 cut(s) 118, 417
AluI AGCT 2 cut(s) 118, 417
Alw21I GWGCWC 3 cut(s) 177, 211, 419
AlwI GGATC 2 cut(s) 5, 248
AlwNI CAGNNNCTG 1 cut(s) 169
AoxI GGCC 2 cut(s) 271, 308
ApeKI GCWGC 2 cut(s) 196, 276
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 1 cut(s) 271
AsuC2I CCSGG 2 cut(s) 184, 254
AsuHPI GGTGA 2 cut(s) 305, 359
BanI GGYRCC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 419
Bbv12I GWGCWC 3 cut(s) 177, 211, 419
BbvI GCAGC 2 cut(s) 208, 263
BccI CCATC 4 cut(s) 119, 220, 396, 402
BceAI ACGGC 1 cut(s) 310
BcgI CGANNNNNNTGC 2 cut(s) 412, 446
BciT130I CCWGG 1 cut(s) 105
BcnI CCSGG 2 cut(s) 184, 254
BfaI CTAG 1 cut(s) 53
BisI GCNGC 6 cut(s) 197, 224, 274, 277, 298, 333
BlsI GCNGC 6 cut(s) 198, 225, 275, 278, 299, 334
Bme1390I CCNGG 3 cut(s) 105, 184, 254
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 3 cut(s) 105, 184, 254
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpuMI CCSGG 2 cut(s) 184, 254
BsaJI CCNNGG 3 cut(s) 103, 292, 493
Bsc4I CCNNNNNNNGG 3 cut(s) 165, 259, 296
Bse1I ACTGG 1 cut(s) 77
Bse3DI GCAATG 1 cut(s) 307
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 103, 292, 493
BseGI GGATG 2 cut(s) 111, 394
BseLI CCNNNNNNNGG 3 cut(s) 165, 259, 296
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 3 cut(s) 425, 512, 522
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 169, 207
BseXI GCAGC 2 cut(s) 208, 263
BseYI CCCAGC 2 cut(s) 135, 350
Bsh1236I CGCG 2 cut(s) 230, 335
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 273, 310
BshNI GGYRCC 1 cut(s) 107
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 3 cut(s) 177, 211, 419
BsiSI CCGG 2 cut(s) 183, 254
BslI CCNNNNNNNGG 3 cut(s) 165, 259, 296
BsnI GGCC 2 cut(s) 273, 310
Bsp1286I GDGCHC 3 cut(s) 177, 211, 419
Bsp143I GATC 2 cut(s) 10, 240
Bsp19I CCATGG 1 cut(s) 493
BspANI GGCC 2 cut(s) 273, 310
BspCNI CTCAG 3 cut(s) 424, 513, 523
BspFNI CGCG 2 cut(s) 230, 335
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 2 cut(s) 5, 248
BspQI GCTCTTC 1 cut(s) 125
BspT107I GGYRCC 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 307
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 3 cut(s) 103, 292, 493
BssMI GATC 2 cut(s) 10, 240
BssT1I CCWWGG 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 4 cut(s) 35, 147, 206, 363
Bst6I CTCTTC 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 3 cut(s) 411, 521, 531
BstDSI CCRYGG 2 cut(s) 292, 493
BstEII GGTNACC 1 cut(s) 525
BstF5I GGATG 2 cut(s) 111, 394
BstFNI CGCG 2 cut(s) 230, 335
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 2 cut(s) 13, 243
BstMBI GATC 2 cut(s) 10, 240
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 2 cut(s) 232, 351
BstNI CCWGG 1 cut(s) 105
BstPI GGTNACC 1 cut(s) 525
BstSCI CCNGG 3 cut(s) 103, 182, 252
BstUI CGCG 2 cut(s) 230, 335
BstV1I GCAGC 2 cut(s) 208, 263
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
BsuRI GGCC 2 cut(s) 273, 310
BtgI CCRYGG 2 cut(s) 292, 493
BtgZI GCGATG 1 cut(s) 374
BtsCI GGATG 2 cut(s) 111, 394
BtsI GCAGTG 2 cut(s) 170, 242
BtsIMutI CAGTG 4 cut(s) 84, 170, 211, 242
Cac8I GCNNGC 1 cut(s) 228
CaiI CAGNNNCTG 1 cut(s) 169
CfoI GCGC 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 271
CviAII CATG 3 cut(s) 379, 434, 494
CviJI RGCY 6 cut(s) 118, 139, 169, 273, 310, 417
CviKI_1 RGCY 6 cut(s) 118, 139, 169, 273, 310, 417
DdeI CTNAG 3 cut(s) 411, 521, 531
DpnI GATC 2 cut(s) 12, 242
DpnII GATC 2 cut(s) 10, 240
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
EciI GGCGGA 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 419
Eco53kI GAGCTC 1 cut(s) 417
Eco91I GGTNACC 1 cut(s) 525
EcoICRI GAGCTC 1 cut(s) 417
EcoO65I GGTNACC 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 3 cut(s) 382, 437, 497
FaiI YATR 6 cut(s) 17, 29, 40, 380, 435, 495
FatI CATG 3 cut(s) 378, 433, 493
Fnu4HI GCNGC 6 cut(s) 197, 224, 274, 277, 298, 333
FokI GGATG 2 cut(s) 98, 381
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 6 cut(s) 197, 224, 274, 277, 298, 333
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 280
GluI GCNGC 6 cut(s) 197, 224, 274, 277, 298, 333
GsaI CCCAGC 2 cut(s) 139, 354
HaeIII GGCC 2 cut(s) 273, 310
HapII CCGG 2 cut(s) 183, 254
HhaI GCGC 1 cut(s) 281
Hin1II CATG 3 cut(s) 382, 437, 497
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 1 cut(s) 424
HpaII CCGG 2 cut(s) 183, 254
HphI GGTGA 2 cut(s) 305, 359
Hpy166II GTNNAC 2 cut(s) 82, 506
Hpy188I TCNGA 1 cut(s) 414
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 2 cut(s) 82, 506
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 2 cut(s) 253, 262
HpyCH4III ACNGT 4 cut(s) 35, 147, 206, 363
HpyCH4V TGCA 1 cut(s) 433
HpyF10VI GCNNNNNNNGC 2 cut(s) 232, 351
HpyF3I CTNAG 3 cut(s) 411, 521, 531
Hsp92II CATG 3 cut(s) 382, 437, 497
HspAI GCGC 1 cut(s) 279
Kzo9I GATC 2 cut(s) 10, 240
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 4 cut(s) 24, 214, 232, 422
LpnPI CCDG 9 cut(s) 90, 90, 117, 128, 149, 155, 196, 267, 364
Lsp1109I GCAGC 2 cut(s) 208, 263
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 3 cut(s) 200, 460, 525
MalI GATC 2 cut(s) 12, 242
MbiI CCGCTC 1 cut(s) 226
MboI GATC 2 cut(s) 10, 240
MboII GAAGA 2 cut(s) 112, 278
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 3 cut(s) 177, 211, 419
MluCI AATT 3 cut(s) 374, 455, 499
MnlI CCTC 7 cut(s) 190, 228, 258, 321, 332, 415, 516
MseI TTAA 2 cut(s) 450, 473
MslI CAYNNNNRTG 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 276
MspI CCGG 2 cut(s) 183, 254
MspR9I CCNGG 3 cut(s) 105, 184, 254
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 2 cut(s) 230, 335
MwoI GCNNNNNNNGC 2 cut(s) 232, 351
NciI CCSGG 2 cut(s) 184, 254
NcoI CCATGG 1 cut(s) 493
NdeII GATC 2 cut(s) 10, 240
NlaIII CATG 3 cut(s) 382, 437, 497
NlaIV GGNNCC 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 125
PfeI GAWTC 1 cut(s) 424
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 6 cut(s) 198, 225, 275, 278, 299, 334
Psp124BI GAGCTC 1 cut(s) 419
Psp6I CCWGG 1 cut(s) 103
PspEI GGTNACC 1 cut(s) 525
PspFI CCCAGC 2 cut(s) 135, 350
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 1 cut(s) 271
PsrI GAACNNNNNNTAC 1 cut(s) 510
PstNI CAGNNNCTG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 1 cut(s) 419
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 2 cut(s) 450, 473
SatI GCNGC 6 cut(s) 197, 224, 274, 277, 298, 333
Sau3AI GATC 2 cut(s) 10, 240
Sau96I GGNCC 1 cut(s) 271
ScrFI CCNGG 3 cut(s) 105, 184, 254
SduI GDGCHC 3 cut(s) 177, 211, 419
SetI ASST 7 cut(s) 120, 264, 324, 407, 419, 527, 532
SmiMI CAYNNNNRTG 1 cut(s) 432
Sse9I AATT 3 cut(s) 374, 455, 499
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 1 cut(s) 419
StyD4I CCNGG 3 cut(s) 103, 182, 252
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 4 cut(s) 35, 147, 206, 363
TaqI TCGA 1 cut(s) 152
TaqII GACCGA 1 cut(s) 173
TasI AATT 3 cut(s) 374, 455, 499
TauI GCSGC 4 cut(s) 226, 276, 300, 335
TfiI GAWTC 1 cut(s) 424
Tru1I TTAA 2 cut(s) 450, 473
Tru9I TTAA 2 cut(s) 450, 473
TscAI CASTG 4 cut(s) 84, 170, 211, 242
TseI GCWGC 2 cut(s) 196, 276
TspDTI ATGAA 2 cut(s) 17, 367
TspRI CASTG 4 cut(s) 84, 170, 211, 242
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.