Rh4AG424300

NADH dehydrogenase ubiquinone 1 beta subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013115
Physical Location & Seq
Forward (+)
72080937 .. 72081218
282 bp
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UTR
Exon/CDS
Intron
Rh4AG424300.1

Sequence Viewer

Length: 282 bp
ATGATAGCGACGCAGGAGGAGATGGTGGAGAATCGGGTGCCGATAGCGTATCGGGACCAGTGCGCGCACTTGCTGATCCCTCTGAACAAGTGCCGCCAGGCCGAGATGTACCTGCCGTGGAAGTGCGGCAACGAGCGCCACGTGTACGAGAAGTGCGAGTACGAGCTCGTCATGGAGCGGATGCTCCTGATGCAGAAGATCCGAGAGAAGGAGGCCCAGAACAAGGCCCAGATTCAGCAGGGACTCGCCATCCCTCTCATTCCCAAGACCGCCGGCGCTTAG

Protein Analysis

93

Amino Acids

10.89

Weight (kDa)

7.66

Isoelectric Point (pI)

21.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUF_B7 PF05676 2 - 61 5.6e-29 NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02050
fragaria_vesca FvH4_4g35970 FvH4_4g35970
malus_domestica MD13G1013300.v1.1 MD16G1010200.v1.1 MD17G1273300.v1.1
prunus_persica Prupe.1G340400_v2.0.a1
pyrus_communis pycom16g01000
rosa_chinensis RchiOBHm_Chr4g0445601
rosa_laevigata RLG00000005726
rosa_multiflora Rmu_ssc0000107.1_g000002
rosa_roxburghii Rroxscaffold_5G00386080
rosa_rugosa Rorug04G0362900
rosa_samantha Rh4AG424300 Rh4BG436400 Rh4CG451000 Rh4DG432900
rosa_wichuraiana Rw4G036380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 37
AccBSI CCGCTC 1 cut(s) 178
AccII CGCG 1 cut(s) 65
AciI CCGC 4 cut(s) 94, 126, 178, 270
AclWI GGATC 2 cut(s) 70, 193
AcvI CACGTG 1 cut(s) 142
AfaI GTAC 3 cut(s) 110, 146, 161
AfiI CCNNNNNNNGG 2 cut(s) 208, 223
AflIII ACRYGT 1 cut(s) 141
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
Alw21I GWGCWC 1 cut(s) 168
AlwI GGATC 2 cut(s) 70, 193
AoxI GGCC 3 cut(s) 99, 213, 225
AspLEI GCGC 4 cut(s) 65, 67, 138, 278
AspS9I GGNCC 3 cut(s) 55, 214, 226
AvaII GGWCC 1 cut(s) 55
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 168
BarI GAAGNNNNNNTAC 2 cut(s) 143, 175
BbrPI CACGTG 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 2 cut(s) 16, 257
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 98
BfoI RGCGCY 2 cut(s) 139, 279
BfuAI ACCTGC 1 cut(s) 120
BisI GCNGC 2 cut(s) 94, 127
BlsI GCNGC 2 cut(s) 95, 128
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 55, 214, 226
BmiI GGNNCC 2 cut(s) 39, 56
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 2 cut(s) 171, 180
BsaAI YACGTR 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 116
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 223
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 58
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 2 cut(s) 186, 249
BseLI CCNNNNNNNGG 2 cut(s) 208, 223
BseNI ACTGG 1 cut(s) 58
BsePI GCGCGC 1 cut(s) 63
BseRI GAGGAG 1 cut(s) 32
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 3 cut(s) 101, 215, 227
BshNI GGYRCC 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 2 cut(s) 68, 255
BslI CCNNNNNNNGG 2 cut(s) 208, 223
BsmFI GGGAC 2 cut(s) 68, 255
BsnI GGCC 3 cut(s) 101, 215, 227
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 2 cut(s) 75, 198
BspACI CCGC 4 cut(s) 94, 126, 178, 270
BspANI GGCC 3 cut(s) 101, 215, 227
BspFNI CGCG 1 cut(s) 65
BspLI GGNNCC 2 cut(s) 39, 56
BspMI ACCTGC 1 cut(s) 120
BspPI GGATC 2 cut(s) 70, 193
BspT107I GGYRCC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 116
BssHII GCGCGC 1 cut(s) 63
BssMI GATC 2 cut(s) 75, 198
Bst2UI CCWGG 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 65, 274
BstDEI CTNAG 1 cut(s) 279
BstDSI CCRYGG 1 cut(s) 116
BstF5I GGATG 2 cut(s) 186, 249
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 2 cut(s) 139, 279
BstHHI GCGC 4 cut(s) 65, 67, 138, 278
BstKTI GATC 2 cut(s) 78, 201
BstMBI GATC 2 cut(s) 75, 198
BstMWI GCNNNNNNNGC 2 cut(s) 135, 190
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstUI CGCG 1 cut(s) 65
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
BsuRI GGCC 3 cut(s) 101, 215, 227
BtgI CCRYGG 1 cut(s) 116
BtsCI GGATG 2 cut(s) 186, 249
BtsIMutI CAGTG 1 cut(s) 65
BveI ACCTGC 1 cut(s) 120
Cac8I GCNNGC 2 cut(s) 65, 274
CfoI GCGC 4 cut(s) 65, 67, 138, 278
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 3 cut(s) 55, 214, 226
CseI GACGC 1 cut(s) 19
Csp6I GTAC 3 cut(s) 109, 145, 160
CviAII CATG 1 cut(s) 172
CviJI RGCY 4 cut(s) 101, 166, 215, 227
CviKI_1 RGCY 4 cut(s) 101, 166, 215, 227
CviQI GTAC 3 cut(s) 109, 145, 160
DdeI CTNAG 1 cut(s) 279
DpnI GATC 2 cut(s) 77, 200
DpnII GATC 2 cut(s) 75, 198
Ecl136II GAGCTC 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 166
Eco72I CACGTG 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 96
EcoT38I GRGCYC 1 cut(s) 168
FaeI CATG 1 cut(s) 175
FaiI YATR 1 cut(s) 173
FaqI GGGAC 2 cut(s) 68, 255
FatI CATG 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 94, 127
FokI GGATG 2 cut(s) 193, 236
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 2 cut(s) 94, 127
GlaI GCGC 4 cut(s) 64, 66, 137, 277
GluI GCNGC 2 cut(s) 94, 127
HaeII RGCGCY 2 cut(s) 139, 279
HaeIII GGCC 3 cut(s) 101, 215, 227
HapII CCGG 1 cut(s) 273
HgaI GACGC 1 cut(s) 19
HhaI GCGC 4 cut(s) 65, 67, 138, 278
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 4 cut(s) 63, 65, 136, 276
HinP1I GCGC 4 cut(s) 63, 65, 136, 276
HinfI GANTC 3 cut(s) 31, 232, 243
HpaII CCGG 1 cut(s) 273
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 84, 203
Hpy188III TCNNGA 2 cut(s) 53, 187
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 1 cut(s) 193
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 190
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 4 cut(s) 63, 65, 136, 276
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Kzo9I GATC 2 cut(s) 75, 198
LmnI GCTCC 2 cut(s) 175, 189
LpnPI CCDG 8 cut(s) 71, 83, 110, 125, 200, 224, 230, 242
LweI GCATC 2 cut(s) 171, 180
MaeII ACGT 1 cut(s) 141
MalI GATC 2 cut(s) 77, 200
MbiI CCGCTC 1 cut(s) 178
MboI GATC 2 cut(s) 75, 198
MboII GAAGA 1 cut(s) 208
MflI RGATCY 1 cut(s) 198
MhlI GDGCHC 1 cut(s) 168
MlyI GAGTC 1 cut(s) 237
MnlI CCTC 4 cut(s) 10, 90, 205, 264
MreI CGCCGGCG 1 cut(s) 272
MroNI GCCGGC 1 cut(s) 272
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 2 cut(s) 135, 190
NaeI GCCGGC 1 cut(s) 274
NdeII GATC 2 cut(s) 75, 198
NgoMIV GCCGGC 1 cut(s) 272
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 2 cut(s) 39, 56
NmeAIII GCCGAG 1 cut(s) 127
PauI GCGCGC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 153
PdiI GCCGGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 31, 232
PkrI GCNGC 2 cut(s) 95, 128
PleI GAGTC 1 cut(s) 237
PmaCI CACGTG 1 cut(s) 142
PmlI CACGTG 1 cut(s) 142
PpsI GAGTC 1 cut(s) 237
Ppu21I YACGTR 1 cut(s) 142
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 96
PspCI CACGTG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 39, 56
PspPI GGNCC 3 cut(s) 55, 214, 226
PsuI RGATCY 1 cut(s) 198
PteI GCGCGC 1 cut(s) 63
RsaI GTAC 3 cut(s) 110, 146, 161
RsaNI GTAC 3 cut(s) 109, 145, 160
SacI GAGCTC 1 cut(s) 168
SatI GCNGC 2 cut(s) 94, 127
Sau3AI GATC 2 cut(s) 75, 198
Sau96I GGNCC 3 cut(s) 55, 214, 226
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 1 cut(s) 168
SetI ASST 3 cut(s) 114, 144, 168
SfaNI GCATC 2 cut(s) 171, 180
SgrAI CRCCGGYG 1 cut(s) 272
SinI GGWCC 1 cut(s) 55
SsiI CCGC 4 cut(s) 94, 126, 178, 270
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 96
TaiI ACGT 1 cut(s) 144
TauI GCSGC 2 cut(s) 96, 129
TfiI GAWTC 2 cut(s) 31, 232
TscAI CASTG 1 cut(s) 65
TspRI CASTG 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.