Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0016144

Physical Location & Seq

Genomic Coordinates Forward (+)

46598630 .. 46601559

Gene Structure 2930 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG294500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2304 bp

ATGTCTTCTTCTTCTTCTTCTTCTTTCATCACAGTGCAATGTTCTCTGCTACCTTCTTGCTCCTCAAGTCATCATCATCCCCCTATAAAAGCTGTAAACTTTGCTCAGATTCCATCATGGGTCTCTCTAAAATCCTCTGCTCCATTGTTGGATACCCAACAATTACAGACTGGGCAAGTCGAAAATGTTCACTTGGTTTCTTTATCGAAACAGGGGAAGATGAAAGAAGCACGAGAATTTCTACAGCAAATGAAGAAGGCTGGTGTGGCGGTTAGTGCTCAGTCGTATAGGTGTTTGTTTGACAAGTGTGGATTGATGGGTTCGTTATCGGATGGCAGATTTGTTCACGAGTGGTTGCGAACAACTGTGAAGAGTCCAGCGGAGTTTCTTGAGAATGATGCTCTTCAGATGTACGGCGATTGTGGGAGTTTAAGGGATGCACAGAAGGTGTTCGATGAAATGCTTCACAGGGATTTGGTTTCTTGGGTTATAATCATATCCGCTTATGTGGAGAAGGGTGTTTTGAACAAAGCTGTTGGTCTGTTTTTGGATATGCTGGAGTCTGGGGTTAGGCCGAATTCGTCTATTTTTACTAGCTTGTTGAAGTCGTTGAATGAGTTTTCGCATTTGGAGTTTGGGAAACAGATGCACTCTTATGTTATAAGAACTGGGAATGCTAGTGGTTCGGTTGAGACGGCGATTGCAAACATGTATGTCAAGTGTGGATGGTTAGATGGTGCTAAACTAGTGTTTGATGAGATGGGTGACAAGAAAAGTGTGGTAGCTTGGACTGGGTTGATGGTGGGGTATACTGAAGATGAGAAGCTGGGGGAGGTTTTAGAATTGTTTGCTGAGATGGTTAGGGGAAGTGTAGAGGTGGATGATTTTGTTTTTTCGATAGTTCTCAAGGCATGTGCAGGTTTGGAGTACTTGAATGCTGGAAGGCAGATCCATGGTTACAGTGTTAAACTTGGATTGGATTCTGAGGTCTCTGTAGGAACTCCTCTAGTGGACTTCTATGTCAAATGTGGAGAATTTGACTCTGCTCGGCGAGCATTTAAGAGGATAAATGAACCAAATGATGTTTCGTGGAGTGCTATAATCTCTGGCTATTGCCAGAACGGTGAATTTGAGGAGTCTCTCGAGATTTTTCAATCTTTAATTGGTAAAGGTGTTACTTTAAATCCATTCATCTATACAAGCATTTTTCAAGCATGCTCTGCAATTGCAGACATAAATTTGGGAGCTCAAGTTCATGCGGATGCAATAAAAAGAGGGTTTGTCGCATTCTTGTATGGAGCAAGTGCAATGATTACGATGTACTCAAAATGTGGCAGATTGGACTATGCCTATCAAGCATTTGAATCTATAGATAAACCTGATACCGTGGCCTGGACTGCTATAATTTGTGGCTATGCATATCATGGCAATGCCTCTGAAGCTCTTCGGCTTTTCAGTAGAATGCAGAACTCTGGCATAAAGCCAAATTCAGTTACATTTGTAGCAGTTCTAACTGCTTGCAGCCATTCAGGTTTGGTAACAGAGGCCAAGCAGTATTTGGATTCAATGAGTAGTGTATATAATGTGGAACCAACCATTGATCATTACGATTGTATGATAGATGTGTACGCTCGTGCTGGACTACTACAGGAGGCACTTGAACTGATAAAGACTATGCCATTTGAGCCCGATGCAATGAGCTGGAAAAGCTTATTGGGTGGATGTTGGATAAATCAAAATCTTGAGCTCGGAAAGTTTGCAGCTGAAAACCTCCTCCAGATGGACCCAGAGGACACTGCTAGTTACATAATCATGTTCAACTTGTATGCATCATCGGGAAAATGGGAAGAAGCCGCCCGTTTTCGAAAAATGATGGCCGAAAAAAACTTGAGGAAAGAAATAGGTTGCAGCTGGATCACTGTCCAGGGCAAAACACACCGGTTTATAGTTGGCGATAAGCACCATTCTCAAATAGACCAGATATACTCAAAGCTAAATGAGCTAAGCATTTCTTTCAAGGATGCTGAAGATTCACTTCTAACTGAGGAGGATGTACTCTCTGGTTTTCCTGAAAGGAAAGAACAGCTTCTTGACCATAGTGAGAGACTTGCTATAGCATTTGGGCTCATTTCCACACCAAGTAATGCCCCTATTATGGTCTTTAAGAACATCAGGGCCTGCAAAGACTGTCATGAGTTTGCAAAGCATGTTTCCATGGTGACAAGGCGTGTAATTATTGTGAGAGACTCCAACAGATTCCATCACTTTAAGTCGGGCGAGTGTTCTTGCAGAGATTATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1900865 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

767

Amino Acids

85.49

Weight (kDa)

5.82

Isoelectric Point (pI)

36.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	67 - 103	5e-06	PPR repeat family
PPR_2	PF13041	158 - 204	5.3e-08	PPR repeat family
PPR_2	PF13041	259 - 306	8.4e-08	PPR repeat family
PPR_2	PF13041	359 - 406	1.6e-07	PPR repeat family
PPR	PF01535	362 - 392	1.7e-07	PPR repeat
PPR_3	PF13812	459 - 507	4.6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	460 - 508	3.4e-13	PPR repeat family
TPR_24	PF23276	461 - 558	4.4e-06	Fungal tetratrico peptide repeats
PPR	PF01535	463 - 493	2.6e-06	PPR repeat
E_motif	PF20431	579 - 639	4.3e-16	E motif
DYW_deaminase	PF14432	691 - 767	2.4e-31	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13270
fragaria_vesca	FvH4_4g22810
malus_domestica	MD13G1129400.v1.1
prunus_persica	Prupe.1G217000_v2.0.a1
pyrus_communis	pycom13g11240
rosa_chinensis	RchiOBHm_Chr4g0429041
rosa_laevigata	RLG00000007101
rosa_multiflora	Rmu_sc0008629.1_g000019
rosa_roxburghii	Rroxscaffold_5G00370690
rosa_rugosa	Rorug04G0232300
rosa_samantha	Rh4AG288300 Rh4BG294500 Rh4CG310000 Rh4DG291400
rosa_wichuraiana	Rw4G024990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	491, 662
AasI	GACNNNNNNGTC	1 cut(s)	1019
Acc36I	ACCTGC	1 cut(s)	908
AccI	GTMKAC	1 cut(s)	809
AciI	CCGC	5 cut(s)	269, 380, 501, 1259, 1854
AclWI	GGATC	2 cut(s)	943, 1922
AcoI	YGGCCR	1 cut(s)	1875
AcsI	RAATTY	6 cut(s)	236, 577, 1034, 1127, 1237, 1486
AcuI	CTGAAG	4 cut(s)	389, 834, 1458, 2046
AfaI	GTAC	5 cut(s)	413, 929, 1322, 1628, 2055
AfiI	CCNNNNNNNGG	3 cut(s)	1392, 1780, 2155
AflIII	ACRYGT	1 cut(s)	708
AgeI	ACCGGT	1 cut(s)	1938
AhlI	ACTAGT	1 cut(s)	745
AjnI	CCWGG	2 cut(s)	1391, 1923
AjuI	GAANNNNNNNTTGG	4 cut(s)	1069, 1101, 1697, 1729
AloI	GAACNNNNNNTCC	2 cut(s)	1236, 1268
Alw21I	GWGCWC	3 cut(s)	280, 1249, 1749
Alw26I	GTCTC	6 cut(s)	127, 686, 994, 1143, 2098, 2238
AlwI	GGATC	2 cut(s)	943, 1922
AlwNI	CAGNNNCTG	1 cut(s)	2178
Ama87I	CYCGRG	1 cut(s)	1142
AoxI	GGCC	5 cut(s)	572, 1389, 1545, 1875, 2175
ApeKI	GCWGC	3 cut(s)	1521, 1760, 1908
ApoI	RAATTY	6 cut(s)	236, 577, 1034, 1127, 1237, 1486
AsiGI	ACCGGT	1 cut(s)	1938
Asp700I	GAANNNNTTC	5 cut(s)	186, 449, 462, 1443, 2085
AspS9I	GGNCC	2 cut(s)	1783, 2175
AsuHPI	GGTGA	3 cut(s)	776, 1136, 2230
AsuII	TTCGAA	1 cut(s)	1864
AvaI	CYCGRG	1 cut(s)	1142
AvaII	GGWCC	1 cut(s)	1783
BanII	GRGCYC	4 cut(s)	1249, 1689, 1749, 2127
BauI	CACGAG	3 cut(s)	231, 347, 1632
Bbv12I	GWGCWC	3 cut(s)	280, 1249, 1749
BbvI	GCAGC	3 cut(s)	1533, 1772, 1920
BceAI	ACGGC	2 cut(s)	430, 711
BciT130I	CCWGG	2 cut(s)	1393, 1925
BciVI	GTATCC	1 cut(s)	145
BclI	TGATCA	1 cut(s)	1600
BcoDI	GTCTC	6 cut(s)	127, 686, 994, 1143, 2098, 2238
BcuI	ACTAGT	1 cut(s)	745
BfaI	CTAG	5 cut(s)	594, 678, 746, 1007, 1800
BfmI	CTRYAG	5 cut(s)	242, 993, 1368, 1646, 2112
BfuAI	ACCTGC	1 cut(s)	908
BfuI	GTATCC	1 cut(s)	145
BisI	GCNGC	4 cut(s)	1522, 1761, 1854, 1909
BlpI	GCTNAGC	1 cut(s)	2003
BlsI	GCNGC	4 cut(s)	1523, 1762, 1855, 1910
BmcAI	AGTACT	1 cut(s)	929
Bme1390I	CCNGG	2 cut(s)	1393, 1925
Bme18I	GGWCC	1 cut(s)	1783
BmeT110I	CYCGRG	1 cut(s)	1142
BmgT120I	GGNCC	2 cut(s)	1783, 2175
BmiI	GGNNCC	2 cut(s)	1590, 1785
BmrFI	CCNGG	2 cut(s)	1393, 1925
BmrI	ACTGGG	3 cut(s)	180, 678, 801
BmsI	GCATC	7 cut(s)	388, 427, 636, 1252, 1681, 1838, 2011
BmuI	ACTGGG	3 cut(s)	180, 678, 801
BpmI	CTGGAG	2 cut(s)	578, 1760
Bpu1102I	GCTNAGC	1 cut(s)	2003
Bpu14I	TTCGAA	1 cut(s)	1864
BpuEI	CTTGAG	6 cut(s)	49, 410, 890, 1233, 1763, 1909
BsaI	GGTCTC	2 cut(s)	127, 994
BsaJI	CCNNGG	4 cut(s)	952, 1386, 1924, 2214
BsaWI	WCCGGW	1 cut(s)	1938
BsaXI	ACNNNNNCTCC	2 cut(s)	1236, 1266
Bsc4I	CCNNNNNNNGG	3 cut(s)	1392, 1780, 2155
Bse118I	RCCGGY	1 cut(s)	1938
Bse1I	ACTGG	3 cut(s)	175, 673, 796
Bse3DI	GCAATG	4 cut(s)	44, 1314, 1435, 1701
BseBI	CCWGG	2 cut(s)	1393, 1925
BseDI	CCNNGG	4 cut(s)	952, 1386, 1924, 2214
BseGI	GGATG	9 cut(s)	76, 337, 442, 731, 886, 1267, 1727, 2026, 2056
BseLI	CCNNNNNNNGG	3 cut(s)	1392, 1780, 2155
BseMI	GCAATG	4 cut(s)	44, 1314, 1435, 1701
BseMII	CTCAG	5 cut(s)	119, 293, 843, 975, 2034
BseNI	ACTGG	3 cut(s)	175, 673, 796
BseRI	GAGGAG	5 cut(s)	52, 993, 1148, 1763, 2060
BseXI	GCAGC	3 cut(s)	1533, 1772, 1920
BseYI	CCCAGC	1 cut(s)	826
BsgI	GTGCAG	1 cut(s)	936
BshFI	GGCC	5 cut(s)	574, 1391, 1547, 1877, 2177
BshTI	ACCGGT	1 cut(s)	1938
BsiHKAI	GWGCWC	3 cut(s)	280, 1249, 1749
BsiHKCI	CYCGRG	1 cut(s)	1142
BsiSI	CCGG	1 cut(s)	1939
BslI	CCNNNNNNNGG	3 cut(s)	1392, 1780, 2155
BsmAI	GTCTC	6 cut(s)	127, 686, 994, 1143, 2098, 2238
BsmBI	CGTCTC	1 cut(s)	686
BsmI	GAATGC	4 cut(s)	679, 940, 1286, 1467
BsnI	GGCC	5 cut(s)	574, 1391, 1547, 1877, 2177
Bso31I	GGTCTC	2 cut(s)	127, 994
BsoBI	CYCGRG	1 cut(s)	1142
Bsp119I	TTCGAA	1 cut(s)	1864
Bsp1286I	GDGCHC	5 cut(s)	280, 1249, 1689, 1749, 2127
Bsp143I	GATC	3 cut(s)	948, 1600, 1914
Bsp1720I	GCTNAGC	1 cut(s)	2003
Bsp19I	CCATGG	2 cut(s)	952, 2214
BspACI	CCGC	5 cut(s)	269, 380, 501, 1259, 1854
BspANI	GGCC	5 cut(s)	574, 1391, 1547, 1877, 2177
BspCNI	CTCAG	5 cut(s)	118, 292, 844, 976, 2035
BspHI	TCATGA	1 cut(s)	2191
BspLI	GGNNCC	2 cut(s)	1590, 1785
BspMI	ACCTGC	1 cut(s)	908
BspPI	GGATC	2 cut(s)	943, 1922
BspQI	GCTCTTC	2 cut(s)	408, 1449
BspT104I	TTCGAA	1 cut(s)	1864
BspTNI	GGTCTC	2 cut(s)	127, 994
BsrDI	GCAATG	4 cut(s)	44, 1314, 1435, 1701
BsrFI	RCCGGY	1 cut(s)	1938
BsrI	ACTGG	3 cut(s)	175, 673, 796
BssAI	RCCGGY	1 cut(s)	1938
BssECI	CCNNGG	4 cut(s)	952, 1386, 1924, 2214
BssMI	GATC	3 cut(s)	948, 1600, 1914
BssNAI	GTATAC	1 cut(s)	810
BssSI	CACGAG	3 cut(s)	231, 347, 1632
BssT1I	CCWWGG	2 cut(s)	952, 2214
Bst1107I	GTATAC	1 cut(s)	810
Bst2BI	CACGAG	3 cut(s)	231, 347, 1632
Bst2UI	CCWGG	2 cut(s)	1393, 1925
Bst4CI	ACNGT	7 cut(s)	34, 367, 962, 1124, 1387, 1921, 2189
Bst6I	CTCTTC	3 cut(s)	365, 408, 1449
BstAPI	GCANNNNNTGC	1 cut(s)	1220
BstBI	TTCGAA	1 cut(s)	1864
BstC8I	GCNNGC	4 cut(s)	1053, 1216, 1519, 2179
BstDEI	CTNAG	6 cut(s)	105, 279, 852, 984, 2003, 2043
BstDSI	CCRYGG	3 cut(s)	952, 1386, 2214
BstF5I	GGATG	9 cut(s)	76, 337, 442, 731, 886, 1267, 1727, 2026, 2056
BstKTI	GATC	3 cut(s)	951, 1603, 1917
BstMAI	GTCTC	6 cut(s)	127, 686, 994, 1143, 2098, 2238
BstMBI	GATC	3 cut(s)	948, 1600, 1914
BstNI	CCWGG	2 cut(s)	1393, 1925
BstNSI	RCATGY	4 cut(s)	712, 915, 1218, 2210
BstSCI	CCNGG	2 cut(s)	1391, 1923
BstSFI	CTRYAG	5 cut(s)	242, 993, 1368, 1646, 2112
BstV1I	GCAGC	3 cut(s)	1533, 1772, 1920
BstX2I	RGATCY	1 cut(s)	948
BstYI	RGATCY	1 cut(s)	948
BstZ17I	GTATAC	1 cut(s)	810
BsuI	GTATCC	1 cut(s)	145
BsuRI	GGCC	5 cut(s)	574, 1391, 1547, 1877, 2177
BtgI	CCRYGG	3 cut(s)	952, 1386, 2214
BtsCI	GGATG	9 cut(s)	76, 337, 442, 731, 886, 1267, 1727, 2026, 2056
BtsI	GCAGTG	1 cut(s)	1794
BtsIMutI	CAGTG	4 cut(s)	39, 967, 1794, 1917
BveI	ACCTGC	1 cut(s)	908
Cac8I	GCNNGC	4 cut(s)	1053, 1216, 1519, 2179
CaiI	CAGNNNCTG	1 cut(s)	2178
CciI	TCATGA	1 cut(s)	2191
Cfr10I	RCCGGY	1 cut(s)	1938
Cfr13I	GGNCC	2 cut(s)	1783, 2175
Csp6I	GTAC	5 cut(s)	412, 928, 1321, 1627, 2054
CspAI	ACCGGT	1 cut(s)	1938
CviQI	GTAC	5 cut(s)	412, 928, 1321, 1627, 2054
DdeI	CTNAG	6 cut(s)	105, 279, 852, 984, 2003, 2043
DpnI	GATC	3 cut(s)	950, 1602, 1916
DpnII	GATC	3 cut(s)	948, 1600, 1914
DraI	TTTAAA	1 cut(s)	1182
DrdI	GACNNNNNNGTC	1 cut(s)	1019
DseDI	GACNNNNNNGTC	1 cut(s)	1019
EaeI	YGGCCR	1 cut(s)	1875
Eam1104I	CTCTTC	3 cut(s)	365, 408, 1449
EarI	CTCTTC	3 cut(s)	365, 408, 1449
Ecl136II	GAGCTC	2 cut(s)	1247, 1747
Eco130I	CCWWGG	2 cut(s)	952, 2214
Eco24I	GRGCYC	4 cut(s)	1249, 1689, 1749, 2127
Eco31I	GGTCTC	2 cut(s)	127, 994
Eco47I	GGWCC	1 cut(s)	1783
Eco53kI	GAGCTC	2 cut(s)	1247, 1747
Eco57I	CTGAAG	4 cut(s)	389, 834, 1458, 2046
Eco88I	CYCGRG	1 cut(s)	1142
EcoICRI	GAGCTC	2 cut(s)	1247, 1747
EcoO109I	RGGNCCY	1 cut(s)	2175
EcoRI	GAATTC	1 cut(s)	577
EcoRII	CCWGG	2 cut(s)	1391, 1923
EcoT14I	CCWWGG	2 cut(s)	952, 2214
EcoT22I	ATGCAT	2 cut(s)	1420, 1831
EcoT38I	GRGCYC	4 cut(s)	1249, 1689, 1749, 2127
ErhI	CCWWGG	2 cut(s)	952, 2214
Esp3I	CGTCTC	1 cut(s)	686
FalI	AAGNNNNNCTT	6 cut(s)	1996, 2028, 2019, 2051, 2070, 2102
FbaI	TGATCA	1 cut(s)	1600
FblI	GTMKAC	1 cut(s)	809
Fnu4HI	GCNGC	4 cut(s)	1522, 1761, 1854, 1909
FokI	GGATG	9 cut(s)	63, 344, 449, 738, 893, 1274, 1734, 2033, 2063
FriOI	GRGCYC	4 cut(s)	1249, 1689, 1749, 2127
Fsp4HI	GCNGC	4 cut(s)	1522, 1761, 1854, 1909
FspBI	CTAG	5 cut(s)	594, 678, 746, 1007, 1800
GluI	GCNGC	4 cut(s)	1522, 1761, 1854, 1909
GsaI	CCCAGC	1 cut(s)	830
GsuI	CTGGAG	2 cut(s)	578, 1760
HaeIII	GGCC	5 cut(s)	574, 1391, 1547, 1877, 2177
HapII	CCGG	1 cut(s)	1939
HindIII	AAGCTT	1 cut(s)	1708
HpaII	CCGG	1 cut(s)	1939
HphI	GGTGA	3 cut(s)	776, 1136, 2230
Hpy166II	GTNNAC	6 cut(s)	97, 190, 346, 810, 1012, 1627
Hpy188I	TCNGA	6 cut(s)	108, 331, 408, 985, 1438, 1751
Hpy8I	GTNNAC	6 cut(s)	97, 190, 346, 810, 1012, 1627
HpyAV	CCTTC	5 cut(s)	63, 250, 439, 508, 936
HpyCH4III	ACNGT	7 cut(s)	34, 367, 962, 1124, 1387, 1921, 2189
HpyF3I	CTNAG	6 cut(s)	105, 279, 852, 984, 2003, 2043
Ksp22I	TGATCA	1 cut(s)	1600
Kzo9I	GATC	3 cut(s)	948, 1600, 1914
LguI	GCTCTTC	2 cut(s)	408, 1449
LmnI	GCTCC	4 cut(s)	65, 145, 1244, 1298
Lsp1109I	GCAGC	3 cut(s)	1533, 1772, 1920
LweI	GCATC	7 cut(s)	388, 427, 636, 1252, 1681, 1838, 2011
MaeI	CTAG	5 cut(s)	594, 678, 746, 1007, 1800
MaeIII	GTNAC	7 cut(s)	764, 956, 1174, 1492, 1537, 1802, 2218
MalI	GATC	3 cut(s)	950, 1602, 1916
MboI	GATC	3 cut(s)	948, 1600, 1914
MfeI	CAATTG	1 cut(s)	1224
MflI	RGATCY	1 cut(s)	948
MhlI	GDGCHC	5 cut(s)	280, 1249, 1689, 1749, 2127
MlyI	GAGTC	5 cut(s)	382, 569, 1034, 1145, 2240
MmeI	TCCRAC	3 cut(s)	129, 1706, 2274
Mph1103I	ATGCAT	2 cut(s)	1420, 1831
MroXI	GAANNNNTTC	5 cut(s)	186, 449, 462, 1443, 2085
MseI	TTAA	7 cut(s)	431, 966, 1059, 1160, 1181, 2163, 2268
MslI	CAYNNNNRTG	5 cut(s)	32, 654, 1260, 1428, 1811
MspA1I	CMGCKG	3 cut(s)	380, 1763, 1911
MspI	CCGG	1 cut(s)	1939
MspR9I	CCNGG	2 cut(s)	1393, 1925
MunI	CAATTG	1 cut(s)	1224
Mva1269I	GAATGC	4 cut(s)	679, 940, 1286, 1467
MvaI	CCWGG	2 cut(s)	1393, 1925
NcoI	CCATGG	2 cut(s)	952, 2214
NdeII	GATC	3 cut(s)	948, 1600, 1914
NlaIV	GGNNCC	2 cut(s)	1590, 1785
NmeAIII	GCCGAG	1 cut(s)	1027
NmuCI	GTSAC	2 cut(s)	764, 2218
NsiI	ATGCAT	2 cut(s)	1420, 1831
NspI	RCATGY	4 cut(s)	712, 915, 1218, 2210
NspV	TTCGAA	1 cut(s)	1864
PaeI	GCATGC	1 cut(s)	1218
PaeR7I	CTCGAG	1 cut(s)	1142
PagI	TCATGA	1 cut(s)	2191
PciI	ACATGT	1 cut(s)	708
PciSI	GCTCTTC	2 cut(s)	408, 1449
PctI	GAATGC	4 cut(s)	679, 940, 1286, 1467
PdmI	GAANNNNTTC	5 cut(s)	186, 449, 462, 1443, 2085
PfeI	GAWTC	6 cut(s)	109, 980, 1364, 1562, 2030, 2256
PinAI	ACCGGT	1 cut(s)	1938
PkrI	GCNGC	4 cut(s)	1523, 1762, 1855, 1910
PleI	GAGTC	5 cut(s)	381, 568, 1034, 1144, 2240
PpsI	GAGTC	5 cut(s)	381, 568, 1034, 1144, 2240
PscI	ACATGT	1 cut(s)	708
PsiI	TTATAA	2 cut(s)	491, 662
Psp124BI	GAGCTC	2 cut(s)	1249, 1749
Psp6I	CCWGG	2 cut(s)	1391, 1923
PspFI	CCCAGC	1 cut(s)	826
PspGI	CCWGG	2 cut(s)	1391, 1923
PspN4I	GGNNCC	2 cut(s)	1590, 1785
PspPI	GGNCC	2 cut(s)	1783, 2175
PstNI	CAGNNNCTG	1 cut(s)	2178
PsuI	RGATCY	1 cut(s)	948
PvuII	CAGCTG	2 cut(s)	1763, 1911
RsaI	GTAC	5 cut(s)	413, 929, 1322, 1628, 2055
RsaNI	GTAC	5 cut(s)	412, 928, 1321, 1627, 2054
RseI	CAYNNNNRTG	5 cut(s)	32, 654, 1260, 1428, 1811
SacI	GAGCTC	2 cut(s)	1249, 1749
SapI	GCTCTTC	2 cut(s)	408, 1449
SaqAI	TTAA	7 cut(s)	431, 966, 1059, 1160, 1181, 2163, 2268
SatI	GCNGC	4 cut(s)	1522, 1761, 1854, 1909
Sau3AI	GATC	3 cut(s)	948, 1600, 1914
Sau96I	GGNCC	2 cut(s)	1783, 2175
ScaI	AGTACT	1 cut(s)	929
SchI	GAGTC	5 cut(s)	382, 569, 1034, 1145, 2240
ScrFI	CCNGG	2 cut(s)	1393, 1925
SduI	GDGCHC	5 cut(s)	280, 1249, 1689, 1749, 2127
SfaNI	GCATC	7 cut(s)	388, 427, 636, 1252, 1681, 1838, 2011
SfcI	CTRYAG	5 cut(s)	242, 993, 1368, 1646, 2112
Sfr274I	CTCGAG	1 cut(s)	1142
SfuI	TTCGAA	1 cut(s)	1864
SinI	GGWCC	1 cut(s)	1783
SlaI	CTCGAG	1 cut(s)	1142
SmiMI	CAYNNNNRTG	5 cut(s)	32, 654, 1260, 1428, 1811
SmlI	CTYRAG	7 cut(s)	64, 389, 905, 1142, 1248, 1742, 1888
SmoI	CTYRAG	7 cut(s)	64, 389, 905, 1142, 1248, 1742, 1888
SpeI	ACTAGT	1 cut(s)	745
SphI	GCATGC	1 cut(s)	1218
SsiI	CCGC	5 cut(s)	269, 380, 501, 1259, 1854
SspMI	CTAG	5 cut(s)	594, 678, 746, 1007, 1800
SstI	GAGCTC	2 cut(s)	1249, 1749
StyD4I	CCNGG	2 cut(s)	1391, 1923
StyI	CCWWGG	2 cut(s)	952, 2214
TaaI	ACNGT	7 cut(s)	34, 367, 962, 1124, 1387, 1921, 2189
TaqI	TCGA	6 cut(s)	180, 206, 453, 896, 1143, 1864
TatI	WGTACW	3 cut(s)	927, 1320, 2053
TauI	GCSGC	1 cut(s)	1856
TfiI	GAWTC	6 cut(s)	109, 980, 1364, 1562, 2030, 2256
Tru1I	TTAA	7 cut(s)	431, 966, 1059, 1160, 1181, 2163, 2268
Tru9I	TTAA	7 cut(s)	431, 966, 1059, 1160, 1181, 2163, 2268
TscAI	CASTG	4 cut(s)	39, 967, 1801, 1924
TseFI	GTSAC	2 cut(s)	764, 2218
TseI	GCWGC	3 cut(s)	1521, 1760, 1908
Tsp45I	GTSAC	2 cut(s)	764, 2218
TspDTI	ATGAA	7 cut(s)	16, 236, 266, 471, 1086, 1180, 1244
TspRI	CASTG	4 cut(s)	39, 967, 1801, 1924
VpaK11BI	GGWCC	1 cut(s)	1783
XapI	RAATTY	6 cut(s)	236, 577, 1034, 1127, 1237, 1486
XceI	RCATGY	4 cut(s)	712, 915, 1218, 2210
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1555
XhoI	CTCGAG	1 cut(s)	1142
XmiI	GTMKAC	1 cut(s)	809
XmnI	GAANNNNTTC	5 cut(s)	186, 449, 462, 1443, 2085
XspI	CTAG	5 cut(s)	594, 678, 746, 1007, 1800
ZrmI	AGTACT	1 cut(s)	929
Zsp2I	ATGCAT	2 cut(s)	1420, 1831

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.