Rh4BG340300

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009397
Physical Location & Seq
Reverse (-)
50219713 .. 50223489
3777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG340300.1

Sequence Viewer

Length: 960 bp
ATGTTGGGAACGGGATTGCAGGTGACGCGTGGCCACGGAGAAGATCGGTTTTACTATCCGCCGAGAGCGCGTAGGGCCCACCAGAGCCAGAAAGCCGCCGAGCAACTTCGCCGAGCTCAGAGCGACGTCACGCCCAGCCAATCGTCTTCGCTCAAGGGCGGCAACCAGAACCGGGAGCCGGAGAACCGGGCCGGGTCGGATGTGCAGCCTCCCAAGCCGGTCGCCGTGCACGCTGGCGAGCCGGCCGTGAAGCCGTTGAGTAATCTCCAGCGGTTCTTGCAGTCCATCACGCCCTCAGTTCCTGCTCAGCACCTCTCCAAGACGACGATGAGAGAATTGAGTACATGTGACGGGGAGTCTCAGCCATACTTTGTTCTTGGTGATCTGTGGGAGTCTTTCAAGGAGTGGAGCGCTTATGGTGCCGGAGTGCCTCTGATTTTGAATGATAGTGACTTTGTGGTCCAGTACTATGTCCCTTATTTATCCGGTATTCAAATATACGGCAACTCTATGAAATTGTCCTCTAAGTCAAGGCGACCAGATGAGGATAGTGACAGTGATTTTCGGGATTCTAGTAGTGATGGTAGCTGTGAATCTGAATCGGATAAAATAAAATATTTGAGGGAGCAGCGGAATCATCAAATGTCGAGTGAAATTCCTCAAAGGTTAGAAAGCATATCTCTGAGAGATCAGCATTATCCACCCCAAGAGGACTGCTCCAGTGATGAGGGTGAACCTGTAAATTCACAAGGTTGCTTATTATTTGAGTATTTTGAACGTGATCTTCCTTATTGCCGTGAGCCTTTAGCTGATAAGATATTAGATCTTGCGTTTCGTTTTCCTGAGCTGAAGACAATAAGAAGTTGTGATCTACTGGCTTCCAGTTGGATTTCTGTTGCATGGTACTATCAAACTCTGCATTTGGTTCAGTGGTTTGTACACAATTATTTGACTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

319

Amino Acids

36.3

Weight (kDa)

5.45

Isoelectric Point (pI)

62.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 87 - 302 1.2e-63 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 10
AasI GACNNNNNNGTC 1 cut(s) 458
AatII GACGTC 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 10
AccB1I GGYRCC 1 cut(s) 419
AccII CGCG 2 cut(s) 28, 70
AciI CCGC 5 cut(s) 59, 96, 159, 271, 631
AcoI YGGCCR 2 cut(s) 31, 243
AcsI RAATTY 2 cut(s) 654, 742
AcuI CTGAAG 1 cut(s) 869
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 4 cut(s) 343, 467, 905, 939
AfeI AGCGCT 1 cut(s) 412
AfiI CCNNNNNNNGG 2 cut(s) 172, 178
AflIII ACRYGT 2 cut(s) 26, 344
AgsI TTSAA 4 cut(s) 400, 442, 494, 776
AhdI GACNNNNNGTC 1 cut(s) 355
AluBI AGCT 4 cut(s) 116, 588, 809, 847
AluI AGCT 4 cut(s) 116, 588, 809, 847
Alw21I GWGCWC 2 cut(s) 118, 231
Alw26I GTCTC 1 cut(s) 363
Alw44I GTGCAC 1 cut(s) 227
AlwNI CAGNNNCTG 1 cut(s) 302
Aor51HI AGCGCT 1 cut(s) 412
AoxI GGCC 4 cut(s) 31, 75, 189, 243
ApaI GGGCCC 1 cut(s) 79
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 2 cut(s) 205, 628
ApoI RAATTY 2 cut(s) 654, 742
AspLEI GCGC 2 cut(s) 70, 413
AspS9I GGNCC 4 cut(s) 75, 76, 189, 460
AsuC2I CCSGG 3 cut(s) 173, 188, 193
AsuHPI GGTGA 3 cut(s) 34, 392, 743
AvaII GGWCC 1 cut(s) 460
BaeGI GKGCMC 2 cut(s) 79, 231
BalI TGGCCA 1 cut(s) 33
BanI GGYRCC 1 cut(s) 419
BanII GRGCYC 2 cut(s) 79, 118
BbsI GAAGAC 2 cut(s) 138, 857
Bbv12I GWGCWC 2 cut(s) 118, 231
BbvI GCAGC 2 cut(s) 217, 640
BccI CCATC 2 cut(s) 293, 575
BceAI ACGGC 5 cut(s) 209, 230, 238, 517, 780
BcnI CCSGG 3 cut(s) 173, 188, 193
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 573
BfoI RGCGCY 1 cut(s) 414
BfuAI ACCTGC 1 cut(s) 10
BglII AGATCT 1 cut(s) 823
BisI GCNGC 4 cut(s) 96, 160, 206, 629
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 4 cut(s) 97, 161, 207, 630
BmcAI AGTACT 1 cut(s) 467
Bme1390I CCNGG 3 cut(s) 173, 188, 193
Bme18I GGWCC 1 cut(s) 460
BmeRI GACNNNNNGTC 1 cut(s) 355
BmgT120I GGNCC 4 cut(s) 75, 76, 189, 460
BmiI GGNNCC 3 cut(s) 77, 177, 421
BmrFI CCNGG 3 cut(s) 173, 188, 193
BpiI GAAGAC 2 cut(s) 138, 857
BplI GAGNNNNNCTC 2 cut(s) 701, 733
BpmI CTGGAG 2 cut(s) 251, 703
Bpu10I CCTNAGC 1 cut(s) 843
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 3 cut(s) 173, 188, 193
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 34
BsaWI WCCGGW 1 cut(s) 485
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 178
Bse118I RCCGGY 2 cut(s) 217, 241
Bse1I ACTGG 4 cut(s) 463, 720, 879, 882
BseDI CCNNGG 1 cut(s) 34
BseGI GGATG 1 cut(s) 205
BseLI CCNNNNNNNGG 2 cut(s) 172, 178
BseMII CTCAG 6 cut(s) 131, 309, 320, 374, 674, 834
BseNI ACTGG 4 cut(s) 463, 720, 879, 882
BseSI GKGCMC 2 cut(s) 79, 231
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 2 cut(s) 217, 640
BseYI CCCAGC 1 cut(s) 134
BsgI GTGCAG 1 cut(s) 224
Bsh1236I CGCG 2 cut(s) 28, 70
Bsh1285I CGRYCG 2 cut(s) 222, 246
BshFI GGCC 4 cut(s) 33, 77, 191, 245
BshNI GGYRCC 1 cut(s) 419
BsiEI CGRYCG 2 cut(s) 222, 246
BsiHKAI GWGCWC 2 cut(s) 118, 231
BsiSI CCGG 8 cut(s) 172, 179, 187, 192, 218, 242, 423, 486
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 2 cut(s) 172, 178
BsmAI GTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 458
BsnI GGCC 4 cut(s) 33, 77, 191, 245
Bsp120I GGGCCC 1 cut(s) 75
Bsp1286I GDGCHC 3 cut(s) 79, 118, 231
Bsp1407I TGTACA 1 cut(s) 937
Bsp143I GATC 6 cut(s) 43, 382, 688, 781, 823, 868
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 5 cut(s) 59, 96, 159, 271, 631
BspANI GGCC 4 cut(s) 33, 77, 191, 245
BspCNI CTCAG 6 cut(s) 130, 308, 319, 373, 675, 835
BspFNI CGCG 2 cut(s) 28, 70
BspLI GGNNCC 3 cut(s) 77, 177, 421
BspMI ACCTGC 1 cut(s) 10
BspT107I GGYRCC 1 cut(s) 419
BsrFI RCCGGY 2 cut(s) 217, 241
BsrGI TGTACA 1 cut(s) 937
BsrI ACTGG 4 cut(s) 463, 720, 879, 882
BssAI RCCGGY 2 cut(s) 217, 241
BssECI CCNNGG 1 cut(s) 34
BssMI GATC 6 cut(s) 43, 382, 688, 781, 823, 868
BssNI GRCGYC 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 557
BstACI GRCGYC 1 cut(s) 126
BstAUI TGTACA 1 cut(s) 937
BstC8I GCNNGC 4 cut(s) 231, 235, 239, 243
BstDEI CTNAG 7 cut(s) 117, 295, 306, 360, 525, 683, 843
BstDSI CCRYGG 1 cut(s) 34
BstF5I GGATG 1 cut(s) 205
BstFNI CGCG 2 cut(s) 28, 70
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 2 cut(s) 70, 413
BstKTI GATC 6 cut(s) 46, 385, 691, 784, 826, 871
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 6 cut(s) 43, 382, 688, 781, 823, 868
BstMCI CGRYCG 2 cut(s) 222, 246
BstMWI GCNNNNNNNGC 7 cut(s) 25, 67, 74, 214, 230, 277, 419
BstNSI RCATGY 1 cut(s) 348
BstSCI CCNGG 3 cut(s) 171, 186, 191
BstSLI GKGCMC 2 cut(s) 79, 231
BstUI CGCG 2 cut(s) 28, 70
BstV1I GCAGC 2 cut(s) 217, 640
BstV2I GAAGAC 2 cut(s) 138, 857
BstX2I RGATCY 1 cut(s) 823
BstYI RGATCY 1 cut(s) 823
BstZI CGGCCG 1 cut(s) 243
BsuRI GGCC 4 cut(s) 33, 77, 191, 245
BtgI CCRYGG 1 cut(s) 34
BtsCI GGATG 1 cut(s) 205
BtsIMutI CAGTG 3 cut(s) 562, 727, 935
BveI ACCTGC 1 cut(s) 10
Cac8I GCNNGC 4 cut(s) 231, 235, 239, 243
CaiI CAGNNNCTG 1 cut(s) 302
CfoI GCGC 2 cut(s) 70, 413
Cfr10I RCCGGY 2 cut(s) 217, 241
Cfr13I GGNCC 4 cut(s) 75, 76, 189, 460
CseI GACGC 1 cut(s) 34
Csp6I GTAC 4 cut(s) 342, 466, 904, 938
CviAII CATG 2 cut(s) 345, 900
CviQI GTAC 4 cut(s) 342, 466, 904, 938
DdeI CTNAG 7 cut(s) 117, 295, 306, 360, 525, 683, 843
DpnI GATC 6 cut(s) 45, 384, 690, 783, 825, 870
DpnII GATC 6 cut(s) 43, 382, 688, 781, 823, 868
DrdI GACNNNNNNGTC 1 cut(s) 458
DriI GACNNNNNGTC 1 cut(s) 355
DseDI GACNNNNNNGTC 1 cut(s) 458
EaeI YGGCCR 2 cut(s) 31, 243
EagI CGGCCG 1 cut(s) 243
Eam1105I GACNNNNNGTC 1 cut(s) 355
EciI GGCGGA 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 116
EclXI CGGCCG 1 cut(s) 243
Eco24I GRGCYC 2 cut(s) 79, 118
Eco47I GGWCC 1 cut(s) 460
Eco47III AGCGCT 1 cut(s) 412
Eco52I CGGCCG 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 869
EcoICRI GAGCTC 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 75
EcoT38I GRGCYC 2 cut(s) 79, 118
FaeI CATG 2 cut(s) 348, 903
FaiI YATR 8 cut(s) 346, 367, 417, 471, 499, 512, 677, 901
FaqI GGGAC 1 cut(s) 458
FatI CATG 2 cut(s) 344, 899
Fnu4HI GCNGC 4 cut(s) 96, 160, 206, 629
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 2 cut(s) 79, 118
Fsp4HI GCNGC 4 cut(s) 96, 160, 206, 629
FspBI CTAG 1 cut(s) 573
GlaI GCGC 2 cut(s) 69, 412
GluI GCNGC 4 cut(s) 96, 160, 206, 629
GsaI CCCAGC 1 cut(s) 138
GsuI CTGGAG 2 cut(s) 251, 703
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 4 cut(s) 33, 77, 191, 245
HapII CCGG 8 cut(s) 172, 179, 187, 192, 218, 242, 423, 486
HgaI GACGC 1 cut(s) 34
HhaI GCGC 2 cut(s) 70, 413
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 2 cut(s) 348, 903
Hin6I GCGC 2 cut(s) 68, 411
HinP1I GCGC 2 cut(s) 68, 411
HinfI GANTC 6 cut(s) 356, 392, 569, 593, 599, 634
HpaII CCGG 8 cut(s) 172, 179, 187, 192, 218, 242, 423, 486
HphI GGTGA 3 cut(s) 34, 392, 743
Hpy166II GTNNAC 3 cut(s) 229, 734, 940
Hpy188I TCNGA 6 cut(s) 120, 199, 435, 598, 604, 684
Hpy188III TCNNGA 2 cut(s) 566, 842
Hpy8I GTNNAC 3 cut(s) 229, 734, 940
Hpy99I CGWCG 2 cut(s) 128, 328
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4IV ACGT 2 cut(s) 126, 778
HpyCH4V TGCA 6 cut(s) 19, 205, 229, 280, 899, 919
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 67, 74, 214, 230, 277, 419
HpyF3I CTNAG 7 cut(s) 117, 295, 306, 360, 525, 683, 843
HpySE526I ACGT 2 cut(s) 126, 778
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 2 cut(s) 348, 903
HspAI GCGC 2 cut(s) 68, 411
KroI GCCGGC 1 cut(s) 241
KroNI GCCGGC 1 cut(s) 243
Kzo9I GATC 6 cut(s) 43, 382, 688, 781, 823, 868
LmnI GCTCC 4 cut(s) 175, 408, 625, 722
Lsp1109I GCAGC 2 cut(s) 217, 640
MaeI CTAG 1 cut(s) 573
MaeII ACGT 2 cut(s) 126, 778
MaeIII GTNAC 5 cut(s) 22, 127, 347, 449, 551
MalI GATC 6 cut(s) 45, 384, 690, 783, 825, 870
MboI GATC 6 cut(s) 43, 382, 688, 781, 823, 868
MboII GAAGA 4 cut(s) 53, 138, 776, 862
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 3 cut(s) 79, 118, 231
MlsI TGGCCA 1 cut(s) 33
MluCI AATT 6 cut(s) 335, 515, 654, 742, 943, 955
MluI ACGCGT 1 cut(s) 26
MluNI TGGCCA 1 cut(s) 33
MlyI GAGTC 2 cut(s) 365, 401
MmeI TCCRAC 2 cut(s) 177, 866
Mox20I TGGCCA 1 cut(s) 33
MroNI GCCGGC 1 cut(s) 241
MscI TGGCCA 1 cut(s) 33
Msp20I TGGCCA 1 cut(s) 33
MspA1I CMGCKG 2 cut(s) 271, 631
MspI CCGG 8 cut(s) 172, 179, 187, 192, 218, 242, 423, 486
MspR9I CCNGG 3 cut(s) 173, 188, 193
MvnI CGCG 2 cut(s) 28, 70
MwoI GCNNNNNNNGC 7 cut(s) 25, 67, 74, 214, 230, 277, 419
NaeI GCCGGC 1 cut(s) 243
NciI CCSGG 3 cut(s) 173, 188, 193
NdeII GATC 6 cut(s) 43, 382, 688, 781, 823, 868
NgoMIV GCCGGC 1 cut(s) 241
NlaIII CATG 2 cut(s) 348, 903
NlaIV GGNNCC 3 cut(s) 77, 177, 421
NmeAIII GCCGAG 3 cut(s) 87, 124, 137
NmuCI GTSAC 5 cut(s) 22, 127, 347, 449, 551
NspI RCATGY 1 cut(s) 348
PaqCI CACCTGC 1 cut(s) 10
PciI ACATGT 1 cut(s) 344
PdiI GCCGGC 1 cut(s) 243
PfeI GAWTC 4 cut(s) 569, 593, 599, 634
PkrI GCNGC 4 cut(s) 97, 161, 207, 630
PleI GAGTC 2 cut(s) 364, 400
PpsI GAGTC 2 cut(s) 364, 400
PscI ACATGT 1 cut(s) 344
Psp124BI GAGCTC 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 134
PspN4I GGNNCC 3 cut(s) 77, 177, 421
PspOMI GGGCCC 1 cut(s) 75
PspPI GGNCC 4 cut(s) 75, 76, 189, 460
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 1 cut(s) 823
RsaI GTAC 4 cut(s) 343, 467, 905, 939
RsaNI GTAC 4 cut(s) 342, 466, 904, 938
SacI GAGCTC 1 cut(s) 118
SatI GCNGC 4 cut(s) 96, 160, 206, 629
Sau3AI GATC 6 cut(s) 43, 382, 688, 781, 823, 868
Sau96I GGNCC 4 cut(s) 75, 76, 189, 460
ScaI AGTACT 1 cut(s) 467
SchI GAGTC 2 cut(s) 365, 401
ScrFI CCNGG 3 cut(s) 173, 188, 193
SduI GDGCHC 3 cut(s) 79, 118, 231
SinI GGWCC 1 cut(s) 460
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 6 cut(s) 335, 515, 654, 742, 943, 955
SsiI CCGC 5 cut(s) 59, 96, 159, 271, 631
SspI AATATT 1 cut(s) 617
SspMI CTAG 1 cut(s) 573
SstI GAGCTC 1 cut(s) 118
StyD4I CCNGG 3 cut(s) 171, 186, 191
TaaI ACNGT 1 cut(s) 557
TaiI ACGT 2 cut(s) 129, 781
TaqI TCGA 1 cut(s) 647
TasI AATT 6 cut(s) 335, 515, 654, 742, 943, 955
TatI WGTACW 3 cut(s) 341, 465, 937
TauI GCSGC 2 cut(s) 98, 162
TfiI GAWTC 4 cut(s) 569, 593, 599, 634
TscAI CASTG 3 cut(s) 562, 727, 935
TseFI GTSAC 5 cut(s) 22, 127, 347, 449, 551
TseI GCWGC 2 cut(s) 205, 628
Tsp45I GTSAC 5 cut(s) 22, 127, 347, 449, 551
TspDTI ATGAA 1 cut(s) 527
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 3 cut(s) 562, 727, 935
VneI GTGCAC 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 2 cut(s) 654, 742
XceI RCATGY 1 cut(s) 348
XspI CTAG 1 cut(s) 573
ZraI GACGTC 1 cut(s) 127
ZrmI AGTACT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.