Rh4CG448600

Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0019927
Physical Location & Seq
Forward (+)
68282882 .. 68298775
15894 bp
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UTR
Exon/CDS
Intron
Rh4CG448600.1

Sequence Viewer

Length: 354 bp
ATGAGCCTCAAAGCCGGCATCGTTGGCCTCCCTAACGTCGGAAAGTCCACGCTTTTCAATGCCGTCGTTGAGAATGGGAAGGCTCAAGCTGCCAATTTTCCATTTTGTACGATAGAGCTAAACGTAGGGATTGTTGCAGTTCCGGATCTGCGCCTGAATGTACTTTCTGATCTCAGCAAGTCTCAGCGCGTAGTCCCGGCATCTTTGGAGCTTGTGGATATTGCTGGCCTTGTGAAGGATGCTAGTCAAGGAGAGGGGTTGGGGAATAAATTCTTGTCAAATATTCGGGAGGTGGACTCCATACTTCAGGGTTTAGGTGCTATTACCAAGTATGTATTACATGACGAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1 YchF-GTPase_C

Protein Analysis

117

Amino Acids

12.32

Weight (kDa)

5.04

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 5 - 104 4.3e-19 50S ribosome-binding GTPase
FeoB_N PF02421 6 - 53 3.1e-07 Ferrous iron transport protein B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019927)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0404851 RchiOBHm_Chr7g0235431
rosa_laevigata RLG00000034227
rosa_samantha Rh3CG159900 Rh3DG160500 Rh4CG448600 Rh7AG445500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 189
AccIII TCCGGA 1 cut(s) 142
AclWI GGATC 1 cut(s) 153
AcsI RAATTY 1 cut(s) 269
AcuI CTGAAG 1 cut(s) 290
AfaI GTAC 2 cut(s) 109, 162
AfiI CCNNNNNNNGG 2 cut(s) 38, 235
AgsI TTSAA 1 cut(s) 58
AluBI AGCT 3 cut(s) 89, 118, 211
AluI AGCT 3 cut(s) 89, 118, 211
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 1 cut(s) 153
Aor13HI TCCGGA 1 cut(s) 142
AoxI GGCC 2 cut(s) 25, 226
ApeKI GCWGC 1 cut(s) 89
ApoI RAATTY 1 cut(s) 269
Asp700I GAANNNNTTC 1 cut(s) 269
AspLEI GCGC 2 cut(s) 153, 189
AsuC2I CCSGG 1 cut(s) 197
BbvI GCAGC 1 cut(s) 76
BceAI ACGGC 1 cut(s) 47
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 2 cut(s) 243, 352
BisI GCNGC 1 cut(s) 90
BlsI GCNGC 1 cut(s) 91
Bme1390I CCNGG 1 cut(s) 197
BmrFI CCNGG 1 cut(s) 197
BmsI GCATC 3 cut(s) 27, 209, 229
BplI GAGNNNNNCTC 2 cut(s) 281, 313
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 197
BsaWI WCCGGW 1 cut(s) 142
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 235
Bse118I RCCGGY 1 cut(s) 14
BseAI TCCGGA 1 cut(s) 142
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 2 cut(s) 38, 235
BseMII CTCAG 2 cut(s) 187, 197
BseXI GCAGC 1 cut(s) 76
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 27, 228
BsiSI CCGG 3 cut(s) 15, 143, 197
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 2 cut(s) 38, 235
BsmAI GTCTC 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 2 cut(s) 27, 228
Bsp13I TCCGGA 1 cut(s) 142
Bsp143I GATC 2 cut(s) 145, 169
BspANI GGCC 2 cut(s) 27, 228
BspCNI CTCAG 2 cut(s) 186, 196
BspEI TCCGGA 1 cut(s) 142
BspFNI CGCG 1 cut(s) 189
BspPI GGATC 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 14
BssMI GATC 2 cut(s) 145, 169
BstC8I GCNNGC 2 cut(s) 16, 226
BstDEI CTNAG 2 cut(s) 173, 183
BstENI CCTNNNNNAGG 1 cut(s) 233
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 2 cut(s) 153, 189
BstKTI GATC 2 cut(s) 148, 172
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 2 cut(s) 145, 169
BstMWI GCNNNNNNNGC 2 cut(s) 24, 89
BstSCI CCNGG 1 cut(s) 195
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BsuRI GGCC 2 cut(s) 27, 228
BtsCI GGATG 1 cut(s) 244
Cac8I GCNNGC 2 cut(s) 16, 226
CfoI GCGC 2 cut(s) 153, 189
Cfr10I RCCGGY 1 cut(s) 14
Csp6I GTAC 2 cut(s) 108, 161
CviAII CATG 1 cut(s) 341
CviJI RGCY 8 cut(s) 6, 14, 27, 83, 89, 118, 211, 228
CviKI_1 RGCY 8 cut(s) 6, 14, 27, 83, 89, 118, 211, 228
CviQI GTAC 2 cut(s) 108, 161
DdeI CTNAG 2 cut(s) 173, 183
DpnI GATC 2 cut(s) 147, 171
DpnII GATC 2 cut(s) 145, 169
Eco57I CTGAAG 1 cut(s) 290
EcoNI CCTNNNNNAGG 1 cut(s) 233
FaeI CATG 1 cut(s) 344
FaiI YATR 3 cut(s) 302, 333, 342
FaqI GGGAC 1 cut(s) 179
FatI CATG 1 cut(s) 340
Fnu4HI GCNGC 1 cut(s) 90
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 1 cut(s) 90
FspBI CTAG 2 cut(s) 243, 352
GlaI GCGC 2 cut(s) 152, 188
GluI GCNGC 1 cut(s) 90
HaeIII GGCC 2 cut(s) 27, 228
HapII CCGG 3 cut(s) 15, 143, 197
HhaI GCGC 2 cut(s) 153, 189
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 2 cut(s) 151, 187
HinP1I GCGC 2 cut(s) 151, 187
HinfI GANTC 1 cut(s) 296
HpaII CCGG 3 cut(s) 15, 143, 197
Hpy166II GTNNAC 2 cut(s) 48, 295
Hpy188I TCNGA 2 cut(s) 41, 169
Hpy188III TCNNGA 2 cut(s) 143, 287
Hpy8I GTNNAC 2 cut(s) 48, 295
Hpy99I CGWCG 2 cut(s) 41, 68
HpyAV CCTTC 2 cut(s) 73, 229
HpyCH4IV ACGT 2 cut(s) 36, 123
HpyCH4V TGCA 1 cut(s) 137
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 89
HpyF3I CTNAG 2 cut(s) 173, 183
HpySE526I ACGT 2 cut(s) 36, 123
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 2 cut(s) 151, 187
Kpn2I TCCGGA 1 cut(s) 142
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Kzo9I GATC 2 cut(s) 145, 169
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 6 cut(s) 28, 156, 167, 210, 210, 293
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 3 cut(s) 27, 209, 229
MaeI CTAG 2 cut(s) 243, 352
MaeII ACGT 2 cut(s) 36, 123
MalI GATC 2 cut(s) 147, 171
MboI GATC 2 cut(s) 145, 169
MflI RGATCY 1 cut(s) 145
MluCI AATT 2 cut(s) 94, 269
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 5 cut(s) 17, 38, 247, 283, 340
MroI TCCGGA 1 cut(s) 142
MroNI GCCGGC 1 cut(s) 14
MroXI GAANNNNTTC 1 cut(s) 269
MspI CCGG 3 cut(s) 15, 143, 197
MspR9I CCNGG 1 cut(s) 197
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 24, 89
NaeI GCCGGC 1 cut(s) 16
NciI CCSGG 1 cut(s) 197
NdeII GATC 2 cut(s) 145, 169
NgoMIV GCCGGC 1 cut(s) 14
NlaIII CATG 1 cut(s) 344
PdiI GCCGGC 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 269
PkrI GCNGC 1 cut(s) 91
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
PsuI RGATCY 1 cut(s) 145
RsaI GTAC 2 cut(s) 109, 162
RsaNI GTAC 2 cut(s) 108, 161
SatI GCNGC 1 cut(s) 90
Sau3AI GATC 2 cut(s) 145, 169
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 197
SetI ASST 7 cut(s) 39, 91, 120, 126, 213, 294, 319
SfaNI GCATC 3 cut(s) 27, 209, 229
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
Sse9I AATT 2 cut(s) 94, 269
SspI AATATT 1 cut(s) 283
SspMI CTAG 2 cut(s) 243, 352
StyD4I CCNGG 1 cut(s) 195
TaiI ACGT 2 cut(s) 39, 126
TasI AATT 2 cut(s) 94, 269
TatI WGTACW 1 cut(s) 160
TseI GCWGC 1 cut(s) 89
XagI CCTNNNNNAGG 1 cut(s) 233
XapI RAATTY 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 2 cut(s) 243, 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.