Rh4DG024100

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017280
Physical Location & Seq
Reverse (-)
4432044 .. 4433543
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG024100.1

Sequence Viewer

Length: 1500 bp
ATGTTGAAGCATTGGAAAACAACATTAGTTCAAAGCTTCACTGTGATTCACCCGTCTGCCAAGTGCTGCATTTTCAGAGTTCCTCAGGTTATTAGGAGACAAAATCCAAAGGCATATCAACCTGATGTTGTCAAAATTGGACCCTTTCATGATCGAGGTAGTAAACACTTCCAACCAATGGAAAATGTGAAGCTCTGGTATTTGCGTAACCTTCTCTTGCGCAAGAATGTAACTTTGAAAACGTTAATTCAAGGCATTAATATTATCGAGTTTGAGAAACATGCCCGTGAGTTTTATGCAGAACCACTTGATCATCTAAACAAGAATGACTTCATTGAAATGATGATACTTGATGGTGTGTTCATAATCGAACTATTTCGGAAGCTTTGGTTTAGTGAAGATATCAGCATCAATGACCCGATATTCAACATGGATTGCATGTTTCAGTATTTATGCCATGACCTTTTGCTACTTGAGAATCAGCTTCCTTGGTTTGTTCTCAACTCTTTATATATCCTTACGAAGGAGACTGTTGATTGCTCCCTGAAAAAACTTCTGCTTGTCATCTTTTACTCAGAACCACAACTGGGACTTAACTCCAATCGCTTCATAGATCATAATACCATTTTCCTAGACACTGATCACTTTGATGATGATCCTGATCATGACGACAAAATTTTGCACATACTTGACCTCATAAGAACTTCAAGTGTTTTCAAATTCAAAGATGATCGGTTTCAGCAATCTTTCTACGGTCCTAACACGCAATTGAAAATACATCCGACTACTACTCTCTCAGACGTCGCAAGTGACATCAGTGACAGCATAGTGAATATAGAATTGGAAGACAGAGTTTATAACAATTTTCACCCGCCATCCTACGGCCTTAAGACACAGTTTATGCATCCGGCCACTACTCTATCAGAGGCAGGAGTCGTATTCAGAATGGATTCCGGCGATAGGAAGAGCATAATGGACATAGAATTCGAAGATGGAGTGTTTACTATTCCACCACTAGTCATTGGAGAGTTGACAGAATCACTCTTCAGGAACTTGATTGCATTTGAGCAATGCTATCATGGATGTTCGCATAAAATAACTTCATATGTGGTTCTAATGGATAACCTCATCGCTTCACAAAAGGATATGAATTTGTTTTGTGAGAAAAACATAATAACTACTAATTGGCGGAGTGCTGAATTAGACACGTATCAATTCTTCAACAAGCTTTATAAGGACACCTCGCTTAAGGAGTTCTTGTATGATGGACTGTGCTACCAAGTAAATGAACATTACCAAGTTGCACAGGACAAAAAGCTTCAAAGAATGAGGCGTGAGCTACGAACTACTGCACAGTTGAAGCGTGATTATCTAACGTGGAATGTTACTTCAACAAGCCTCTGGAAAGTTACTTCTTTGATTGCAGCTTCTGTCCTTTTGGGAGTCACCATACTTCAAACCATATATGCCATGAAGCAATATTACTATTCCCTTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

499

Amino Acids

58.64

Weight (kDa)

6.19

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 24 - 480 1.7e-95 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017280)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02490 FvH4_4g02490 FvH4_4g02490 FvH4_4g02490 FvH4_4g02490
rosa_chinensis RchiOBHm_Chr4g0389931
rosa_laevigata RLG00000009944 RLG00000015347
rosa_multiflora Rmu_sc0004336.1_g000027
rosa_roxburghii Rroxscaffold_5G00336000
rosa_rugosa Rorug03G0327200
rosa_samantha Rh4DG024100
rosa_wichuraiana Rw4G002310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 858, 1233
AatII GACGTC 1 cut(s) 804
Acc16I TGCGCA 1 cut(s) 221
AccB7I CCANNNNNTGG 1 cut(s) 178
AciI CCGC 2 cut(s) 872, 1189
AclI AACGTT 1 cut(s) 242
AclWI GGATC 1 cut(s) 650
AcoI YGGCCR 1 cut(s) 909
AcsI RAATTY 4 cut(s) 675, 719, 983, 1150
AcuI CTGAAG 1 cut(s) 1030
AcyI GRCGYC 1 cut(s) 801
AfiI CCNNNNNNNGG 5 cut(s) 178, 523, 587, 881, 960
AflII CTTAAG 2 cut(s) 887, 1247
AflIII ACRYGT 1 cut(s) 1206
AhlI ACTAGT 1 cut(s) 1015
AjuI GAANNNNNNNTTGG 2 cut(s) 824, 856
AluBI AGCT 8 cut(s) 36, 193, 385, 484, 1228, 1318, 1339, 1427
AluI AGCT 8 cut(s) 36, 193, 385, 484, 1228, 1318, 1339, 1427
Alw26I GTCTC 2 cut(s) 91, 521
AlwI GGATC 1 cut(s) 650
AlwNI CAGNNNCTG 1 cut(s) 1430
AoxI GGCC 2 cut(s) 883, 909
ApeKI GCWGC 2 cut(s) 66, 1424
ApoI RAATTY 4 cut(s) 675, 719, 983, 1150
ArsI GACNNNNNNTTYG 2 cut(s) 968, 1000
AseI ATTAAT 1 cut(s) 258
Asp700I GAANNNNTTC 3 cut(s) 329, 375, 949
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 2 cut(s) 140, 755
AsuHPI GGTGA 3 cut(s) 41, 860, 1438
AsuII TTCGAA 1 cut(s) 987
AvaII GGWCC 2 cut(s) 140, 755
AxyI CCTNAGG 1 cut(s) 84
BarI GAAGNNNNNNTAC 4 cut(s) 1202, 1234, 1466, 1498
BbsI GAAGAC 1 cut(s) 852
BbvI GCAGC 2 cut(s) 53, 1436
BccI CCATC 4 cut(s) 347, 883, 986, 1259
BceAI ACGGC 1 cut(s) 898
BclI TGATCA 3 cut(s) 310, 640, 661
BcoDI GTCTC 2 cut(s) 91, 521
BcuI ACTAGT 1 cut(s) 1015
BfaI CTAG 2 cut(s) 632, 1016
BfrI CTTAAG 2 cut(s) 887, 1247
BisI GCNGC 2 cut(s) 67, 1425
BlsI GCNGC 2 cut(s) 68, 1426
Bme18I GGWCC 2 cut(s) 140, 755
BmgT120I GGNCC 2 cut(s) 140, 755
BmiI GGNNCC 1 cut(s) 142
BmrI ACTGGG 1 cut(s) 596
BmsI GCATC 2 cut(s) 417, 913
BmuI ACTGGG 1 cut(s) 596
BpiI GAAGAC 1 cut(s) 852
Bpu14I TTCGAA 1 cut(s) 987
BpuEI CTTGAG 1 cut(s) 494
BsaAI YACGTR 1 cut(s) 1209
BsaBI GATNNNNATC 2 cut(s) 654, 660
BsaHI GRCGYC 1 cut(s) 801
BsaJI CCNNGG 1 cut(s) 488
BsaXI ACNNNNNCTCC 2 cut(s) 1244, 1274
Bsc4I CCNNNNNNNGG 5 cut(s) 178, 523, 587, 881, 960
Bse1I ACTGG 1 cut(s) 591
Bse21I CCTNAGG 1 cut(s) 84
Bse3DI GCAATG 1 cut(s) 1076
Bse8I GATNNNNATC 2 cut(s) 654, 660
BseDI CCNNGG 1 cut(s) 488
BseGI GGATG 4 cut(s) 778, 875, 904, 1088
BseJI GATNNNNATC 2 cut(s) 654, 660
BseLI CCNNNNNNNGG 5 cut(s) 178, 523, 587, 881, 960
BseMI GCAATG 1 cut(s) 1076
BseMII CTCAG 3 cut(s) 98, 588, 810
BseNI ACTGG 1 cut(s) 591
BseXI GCAGC 2 cut(s) 53, 1436
BsgI GTGCAG 1 cut(s) 1335
BshFI GGCC 2 cut(s) 885, 911
BsiSI CCGG 2 cut(s) 908, 954
BslFI GGGAC 1 cut(s) 603
BslI CCNNNNNNNGG 5 cut(s) 178, 523, 587, 881, 960
BsmAI GTCTC 2 cut(s) 91, 521
BsmFI GGGAC 1 cut(s) 603
BsnI GGCC 2 cut(s) 885, 911
Bsp119I TTCGAA 1 cut(s) 987
Bsp143I GATC 7 cut(s) 151, 310, 613, 640, 655, 661, 730
BspACI CCGC 2 cut(s) 872, 1189
BspANI GGCC 2 cut(s) 885, 911
BspCNI CTCAG 3 cut(s) 97, 587, 809
BspHI TCATGA 2 cut(s) 148, 664
BspLI GGNNCC 1 cut(s) 142
BspPI GGATC 1 cut(s) 650
BspQI GCTCTTC 1 cut(s) 959
BspT104I TTCGAA 1 cut(s) 987
BspTI CTTAAG 2 cut(s) 887, 1247
BsrDI GCAATG 1 cut(s) 1076
BsrI ACTGG 1 cut(s) 591
BssECI CCNNGG 1 cut(s) 488
BssMI GATC 7 cut(s) 151, 310, 613, 640, 655, 661, 730
BssNI GRCGYC 1 cut(s) 801
BssT1I CCWWGG 1 cut(s) 488
Bst4CI ACNGT 6 cut(s) 43, 532, 755, 897, 1272, 1356
Bst6I CTCTTC 2 cut(s) 959, 1049
BstACI GRCGYC 1 cut(s) 801
BstAFI CTTAAG 2 cut(s) 887, 1247
BstBAI YACGTR 1 cut(s) 1209
BstBI TTCGAA 1 cut(s) 987
BstDEI CTNAG 3 cut(s) 84, 574, 796
BstENI CCTNNNNNAGG 1 cut(s) 521
BstF5I GGATG 4 cut(s) 778, 875, 904, 1088
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 7 cut(s) 154, 313, 616, 643, 658, 664, 733
BstMAI GTCTC 2 cut(s) 91, 521
BstMBI GATC 7 cut(s) 151, 310, 613, 640, 655, 661, 730
BstNSI RCATGY 2 cut(s) 284, 442
BstV1I GCAGC 2 cut(s) 53, 1436
BstV2I GAAGAC 1 cut(s) 852
Bsu36I CCTNAGG 1 cut(s) 84
BsuRI GGCC 2 cut(s) 885, 911
BtgZI GCGATG 1 cut(s) 1114
BtsCI GGATG 4 cut(s) 778, 875, 904, 1088
BtsIMutI CAGTG 3 cut(s) 39, 636, 823
CaiI CAGNNNCTG 1 cut(s) 1430
CciI TCATGA 2 cut(s) 148, 664
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 140, 755
CviAII CATG 8 cut(s) 149, 281, 430, 439, 458, 665, 1079, 1471
DdeI CTNAG 3 cut(s) 84, 574, 796
DpnI GATC 7 cut(s) 153, 312, 615, 642, 657, 663, 732
DpnII GATC 7 cut(s) 151, 310, 613, 640, 655, 661, 730
EaeI YGGCCR 1 cut(s) 909
Eam1104I CTCTTC 2 cut(s) 959, 1049
EarI CTCTTC 2 cut(s) 959, 1049
EciI GGCGGA 1 cut(s) 1204
Eco130I CCWWGG 1 cut(s) 488
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 2 cut(s) 140, 755
Eco57I CTGAAG 1 cut(s) 1030
Eco81I CCTNAGG 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoRI GAATTC 1 cut(s) 983
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 488
EcoT22I ATGCAT 1 cut(s) 906
ErhI CCWWGG 1 cut(s) 488
FaeI CATG 8 cut(s) 152, 284, 433, 442, 461, 668, 1082, 1474
FalI AAGNNNNNCTT 4 cut(s) 314, 346, 1241, 1273
FaqI GGGAC 1 cut(s) 603
FatI CATG 8 cut(s) 148, 280, 429, 438, 457, 664, 1078, 1470
FauI CCCGC 1 cut(s) 879
FauNDI CATATG 1 cut(s) 1105
FbaI TGATCA 3 cut(s) 310, 640, 661
Fnu4HI GCNGC 2 cut(s) 67, 1425
FokI GGATG 4 cut(s) 765, 862, 891, 1095
Fsp4HI GCNGC 2 cut(s) 67, 1425
FspBI CTAG 2 cut(s) 632, 1016
FspI TGCGCA 1 cut(s) 221
GlaI GCGC 1 cut(s) 221
GluI GCNGC 2 cut(s) 67, 1425
HaeIII GGCC 2 cut(s) 885, 911
HapII CCGG 2 cut(s) 908, 954
HhaI GCGC 1 cut(s) 222
Hin1I GRCGYC 1 cut(s) 801
Hin1II CATG 8 cut(s) 152, 284, 433, 442, 461, 668, 1082, 1474
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HincII GTYRAC 1 cut(s) 1032
HindII GTYRAC 1 cut(s) 1032
HindIII AAGCTT 4 cut(s) 34, 383, 1226, 1316
HinfI GANTC 6 cut(s) 46, 478, 933, 950, 1037, 1443
HpaII CCGG 2 cut(s) 908, 954
HphI GGTGA 3 cut(s) 41, 860, 1438
Hpy166II GTNNAC 3 cut(s) 164, 1002, 1032
Hpy188I TCNGA 7 cut(s) 77, 381, 577, 783, 799, 925, 944
Hpy188III TCNNGA 5 cut(s) 149, 659, 665, 1048, 1402
Hpy8I GTNNAC 3 cut(s) 164, 1002, 1032
Hpy99I CGWCG 1 cut(s) 806
HpyAV CCTTC 2 cut(s) 221, 517
HpyCH4III ACNGT 6 cut(s) 43, 532, 755, 897, 1272, 1356
HpyCH4IV ACGT 4 cut(s) 242, 801, 1208, 1376
HpyCH4V TGCA 9 cut(s) 69, 299, 438, 682, 904, 1061, 1304, 1352, 1424
HpyF3I CTNAG 3 cut(s) 84, 574, 796
HpySE526I ACGT 4 cut(s) 242, 801, 1208, 1376
Hsp92I GRCGYC 1 cut(s) 801
Hsp92II CATG 8 cut(s) 152, 284, 433, 442, 461, 668, 1082, 1474
HspAI GCGC 1 cut(s) 220
Ksp22I TGATCA 3 cut(s) 310, 640, 661
Kzo9I GATC 7 cut(s) 151, 310, 613, 640, 655, 661, 730
LguI GCTCTTC 1 cut(s) 959
LmnI GCTCC 1 cut(s) 545
Lsp1109I GCAGC 2 cut(s) 53, 1436
LweI GCATC 2 cut(s) 417, 913
MaeI CTAG 2 cut(s) 632, 1016
MaeII ACGT 4 cut(s) 242, 801, 1208, 1376
MaeIII GTNAC 7 cut(s) 206, 229, 809, 818, 1384, 1408, 1444
MalI GATC 7 cut(s) 153, 312, 615, 642, 657, 663, 732
MboI GATC 7 cut(s) 151, 310, 613, 640, 655, 661, 730
MboII GAAGA 6 cut(s) 410, 857, 976, 1001, 1036, 1210
MfeI CAATTG 1 cut(s) 767
MlyI GAGTC 2 cut(s) 942, 1452
MmeI TCCRAC 2 cut(s) 196, 806
MnlI CCTC 8 cut(s) 93, 149, 704, 919, 1136, 1252, 1323, 1409
Mph1103I ATGCAT 1 cut(s) 906
MroXI GAANNNNTTC 3 cut(s) 329, 375, 949
MseI TTAA 5 cut(s) 245, 258, 594, 888, 1248
MslI CAYNNNNRTG 3 cut(s) 285, 338, 648
MspCI CTTAAG 2 cut(s) 887, 1247
MspI CCGG 2 cut(s) 908, 954
MunI CAATTG 1 cut(s) 767
NdeI CATATG 1 cut(s) 1105
NdeII GATC 7 cut(s) 151, 310, 613, 640, 655, 661, 730
NlaIII CATG 8 cut(s) 152, 284, 433, 442, 461, 668, 1082, 1474
NlaIV GGNNCC 1 cut(s) 142
NmuCI GTSAC 3 cut(s) 809, 818, 1444
NsbI TGCGCA 1 cut(s) 221
NsiI ATGCAT 1 cut(s) 906
NspI RCATGY 2 cut(s) 284, 442
NspV TTCGAA 1 cut(s) 987
PagI TCATGA 2 cut(s) 148, 664
PciSI GCTCTTC 1 cut(s) 959
PdmI GAANNNNTTC 3 cut(s) 329, 375, 949
PfeI GAWTC 4 cut(s) 46, 478, 950, 1037
PflMI CCANNNNNTGG 1 cut(s) 178
PkrI GCNGC 2 cut(s) 68, 1426
PleI GAGTC 2 cut(s) 941, 1451
PpsI GAGTC 2 cut(s) 941, 1451
Ppu21I YACGTR 1 cut(s) 1209
PshBI ATTAAT 1 cut(s) 258
PsiI TTATAA 2 cut(s) 858, 1233
Psp1406I AACGTT 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 142
PspPI GGNCC 2 cut(s) 140, 755
PstNI CAGNNNCTG 1 cut(s) 1430
RseI CAYNNNNRTG 3 cut(s) 285, 338, 648
SapI GCTCTTC 1 cut(s) 959
SaqAI TTAA 5 cut(s) 245, 258, 594, 888, 1248
SatI GCNGC 2 cut(s) 67, 1425
Sau3AI GATC 7 cut(s) 151, 310, 613, 640, 655, 661, 730
Sau96I GGNCC 2 cut(s) 140, 755
SchI GAGTC 2 cut(s) 942, 1452
SfaNI GCATC 2 cut(s) 417, 913
SfuI TTCGAA 1 cut(s) 987
SinI GGWCC 2 cut(s) 140, 755
SmiMI CAYNNNNRTG 3 cut(s) 285, 338, 648
SmlI CTYRAG 3 cut(s) 473, 887, 1247
SmoI CTYRAG 3 cut(s) 473, 887, 1247
SpeI ACTAGT 1 cut(s) 1015
SsiI CCGC 2 cut(s) 872, 1189
SspI AATATT 2 cut(s) 262, 1481
SspMI CTAG 2 cut(s) 632, 1016
StyI CCWWGG 1 cut(s) 488
TaaI ACNGT 6 cut(s) 43, 532, 755, 897, 1272, 1356
TaiI ACGT 4 cut(s) 245, 804, 1211, 1379
TaqI TCGA 5 cut(s) 154, 267, 369, 987, 1495
TfiI GAWTC 4 cut(s) 46, 478, 950, 1037
Tru1I TTAA 5 cut(s) 245, 258, 594, 888, 1248
Tru9I TTAA 5 cut(s) 245, 258, 594, 888, 1248
TscAI CASTG 3 cut(s) 46, 643, 823
TseFI GTSAC 3 cut(s) 809, 818, 1444
TseI GCWGC 2 cut(s) 66, 1424
Tsp45I GTSAC 3 cut(s) 809, 818, 1444
TspDTI ATGAA 8 cut(s) 137, 322, 352, 598, 1092, 1163, 1302, 1487
TspRI CASTG 3 cut(s) 46, 643, 823
Van91I CCANNNNNTGG 1 cut(s) 178
Vha464I CTTAAG 2 cut(s) 887, 1247
VpaK11BI GGWCC 2 cut(s) 140, 755
VspI ATTAAT 1 cut(s) 258
XagI CCTNNNNNAGG 1 cut(s) 521
XapI RAATTY 4 cut(s) 675, 719, 983, 1150
XceI RCATGY 2 cut(s) 284, 442
XmnI GAANNNNTTC 3 cut(s) 329, 375, 949
XspI CTAG 2 cut(s) 632, 1016
ZraI GACGTC 1 cut(s) 802
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.