Rw4G002310

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017280
Physical Location & Seq
Reverse (-)
4442437 .. 4443957
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G002310.1

Sequence Viewer

Length: 1521 bp
ATGGAAGACGATAAAGAAATCAATGTTGAAGCATTGGAAAACAACATTAGTGCAAAGCTTCACTGTGATTCACCCCTGTCTGCCAAGTGCTGCATTTTCAGAGTTCCCCAGGTTATTAGGAGACAAAATCCAAAGGCATATCAACCTGATGTTGTCACAATTGGACCCTTTCATGATCGAGGCAGTAAACACTTCCAACCCATGGAAAATGTGAAGCTCTGGTATTTGCGTAACCATCTCTTGCGCAAGAATATAACTTTGAAAACGTTAATTCAAGGCATTAATATTATCGAGTTTGAGAAACATGCCCGTGAGTTTTATGCAGAACCACTTGATCATCTAAACAAGAATGACTTCATTGAAATGATGATACTTGATGGTTTGTTCATAATCGAACTATTTCGGAAGCTTTGGCTCAGTGAAGATATCAGCATCAATGACCCGATATTCAACATGGATTGCATGTTTCAGTATTTATGCCATGACATTTTGCTACTTGAGAATCAGCTTCCTTGGTTTGTTCTCAACTCTTTATATATCCTTACGAAGGAGACTGTTGATTGCTCCCTGAAAAAACTTCTGCTTGTCATCTTTTACTCAGAACCACAACTGGGACTTAACTCCAATCGCTTCATAGATCATAATACCATTTTCCTAGACACTGACTTTGATGATGATCCTGATCATGATGACAAAATTTTGCACATACTTGACCTCATAAGAACTTCAAGTGTTTTCAAATTCAAAGATGATCGGTTTCAGCAATCTTTCTACGGTCCTAACACGCAATTGAAAATACATCCGACTACTACTCTCTCAGACGTCGCAAGTGACATCAGTGACAGCATAGTGAATATAGAATTGGAAGACAGAGTTTATAACAATTTTCACCCGCCATCCTACGGCCTTAAGACACAGTTTATGCATCCGGCCACTACTCTATCAGAGGCAGGAGTCGTATTCAGAATGGATTCCGGCGATAGGAAGAGCATAATGGACATAGAATTCGAAGATGGAGTGTTTACTATTCCACCACTAGTCATTGGAGAGTTGACAGAATCACTCTTCAGGAACTTGATTGCATTTGAGCAATGCTATCATGGATGTTCGCATAAAATAACTTCATATGTGGTTCTAATGGATAACCTCATCGCTTCACAAAAGGATATGCATTTGCTTTGTGAGAAAAACATAATAACTACTAATTGGCGGAGTGCTGAATTAGACACGTATCAATTCTTCAACAAGCTTTATAAGGACACCTCGCTTAAGGAGTTCTTGTATGATGGACTGTGCTACCAAGTAAATGAACATTACCAAGTTGCACAGGACAAAAAGCTTCAAAGAATGAGGCGCGAGCTAAGAACTACTGCACAGTTGAAGCGTGATTATCTAACGTGGAATGTTACTTCAACAAGCCTCTGGAAAGTTACTTCTTTGATTGCAGCTTCTGTCCTTTTGGGAGTCACTATACTTCAAACCATATATGCCATGAAGCAATATTATTATTCCCCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

506

Amino Acids

59.23

Weight (kDa)

5.59

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 32 - 487 1.5e-95 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017280)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02490 FvH4_4g02490 FvH4_4g02490 FvH4_4g02490 FvH4_4g02490
rosa_chinensis RchiOBHm_Chr4g0389931
rosa_laevigata RLG00000009944 RLG00000015347
rosa_multiflora Rmu_sc0004336.1_g000027
rosa_roxburghii Rroxscaffold_5G00336000
rosa_rugosa Rorug03G0327200
rosa_samantha Rh4DG024100
rosa_wichuraiana Rw4G002310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 879, 1254
AatII GACGTC 1 cut(s) 825
Acc16I TGCGCA 1 cut(s) 245
AccB7I CCANNNNNTGG 1 cut(s) 202
AccII CGCG 1 cut(s) 1356
AciI CCGC 2 cut(s) 893, 1210
AclI AACGTT 1 cut(s) 266
AclWI GGATC 1 cut(s) 671
AcoI YGGCCR 1 cut(s) 930
AcsI RAATTY 3 cut(s) 696, 740, 1004
AcuI CTGAAG 1 cut(s) 1051
AcyI GRCGYC 1 cut(s) 822
AfiI CCNNNNNNNGG 5 cut(s) 202, 547, 611, 902, 981
AflII CTTAAG 2 cut(s) 908, 1268
AflIII ACRYGT 1 cut(s) 1227
AhlI ACTAGT 1 cut(s) 1036
AjnI CCWGG 1 cut(s) 108
AjuI GAANNNNNNNTTGG 2 cut(s) 845, 877
AluBI AGCT 8 cut(s) 58, 217, 409, 508, 1249, 1339, 1360, 1448
AluI AGCT 8 cut(s) 58, 217, 409, 508, 1249, 1339, 1360, 1448
Alw26I GTCTC 2 cut(s) 115, 545
AlwI GGATC 1 cut(s) 671
AlwNI CAGNNNCTG 1 cut(s) 1451
AoxI GGCC 2 cut(s) 904, 930
ApeKI GCWGC 2 cut(s) 90, 1445
ApoI RAATTY 3 cut(s) 696, 740, 1004
ArsI GACNNNNNNTTYG 4 cut(s) 650, 682, 989, 1021
AseI ATTAAT 1 cut(s) 282
Asp700I GAANNNNTTC 3 cut(s) 353, 399, 970
AspLEI GCGC 2 cut(s) 246, 1356
AspS9I GGNCC 2 cut(s) 164, 776
AsuHPI GGTGA 2 cut(s) 63, 881
AsuII TTCGAA 1 cut(s) 1008
AvaII GGWCC 2 cut(s) 164, 776
BarI GAAGNNNNNNTAC 2 cut(s) 1223, 1255
BbsI GAAGAC 2 cut(s) 12, 873
BbvI GCAGC 2 cut(s) 77, 1457
BccI CCATC 5 cut(s) 243, 371, 904, 1007, 1280
BceAI ACGGC 1 cut(s) 919
BciT130I CCWGG 1 cut(s) 110
BclI TGATCA 2 cut(s) 334, 682
BcoDI GTCTC 2 cut(s) 115, 545
BcuI ACTAGT 1 cut(s) 1036
BfaI CTAG 2 cut(s) 656, 1037
BfrI CTTAAG 2 cut(s) 908, 1268
BisI GCNGC 2 cut(s) 91, 1446
BlsI GCNGC 2 cut(s) 92, 1447
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 2 cut(s) 164, 776
BmgT120I GGNCC 2 cut(s) 164, 776
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 1 cut(s) 110
BmrI ACTGGG 1 cut(s) 620
BmsI GCATC 2 cut(s) 441, 934
BmuI ACTGGG 1 cut(s) 620
BpiI GAAGAC 2 cut(s) 12, 873
Bpu14I TTCGAA 1 cut(s) 1008
BpuEI CTTGAG 1 cut(s) 518
BsaAI YACGTR 1 cut(s) 1230
BsaBI GATNNNNATC 2 cut(s) 675, 681
BsaHI GRCGYC 1 cut(s) 822
BsaJI CCNNGG 3 cut(s) 108, 201, 512
BsaXI ACNNNNNCTCC 2 cut(s) 1265, 1295
Bsc4I CCNNNNNNNGG 5 cut(s) 202, 547, 611, 902, 981
Bse1I ACTGG 1 cut(s) 615
Bse3DI GCAATG 1 cut(s) 1097
Bse8I GATNNNNATC 2 cut(s) 675, 681
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 3 cut(s) 108, 201, 512
BseGI GGATG 4 cut(s) 799, 896, 925, 1109
BseJI GATNNNNATC 2 cut(s) 675, 681
BseLI CCNNNNNNNGG 5 cut(s) 202, 547, 611, 902, 981
BseMI GCAATG 1 cut(s) 1097
BseMII CTCAG 3 cut(s) 430, 612, 831
BseNI ACTGG 1 cut(s) 615
BseXI GCAGC 2 cut(s) 77, 1457
BsgI GTGCAG 1 cut(s) 1356
Bsh1236I CGCG 1 cut(s) 1356
BshFI GGCC 2 cut(s) 906, 932
BsiSI CCGG 2 cut(s) 929, 975
BslFI GGGAC 1 cut(s) 627
BslI CCNNNNNNNGG 5 cut(s) 202, 547, 611, 902, 981
BsmAI GTCTC 2 cut(s) 115, 545
BsmFI GGGAC 1 cut(s) 627
BsnI GGCC 2 cut(s) 906, 932
Bsp119I TTCGAA 1 cut(s) 1008
Bsp143I GATC 6 cut(s) 175, 334, 637, 676, 682, 751
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 2 cut(s) 893, 1210
BspANI GGCC 2 cut(s) 906, 932
BspCNI CTCAG 3 cut(s) 429, 611, 830
BspFNI CGCG 1 cut(s) 1356
BspHI TCATGA 2 cut(s) 172, 685
BspLI GGNNCC 1 cut(s) 166
BspPI GGATC 1 cut(s) 671
BspQI GCTCTTC 1 cut(s) 980
BspT104I TTCGAA 1 cut(s) 1008
BspTI CTTAAG 2 cut(s) 908, 1268
BsrDI GCAATG 1 cut(s) 1097
BsrI ACTGG 1 cut(s) 615
BssECI CCNNGG 3 cut(s) 108, 201, 512
BssMI GATC 6 cut(s) 175, 334, 637, 676, 682, 751
BssNI GRCGYC 1 cut(s) 822
BssT1I CCWWGG 2 cut(s) 201, 512
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 6 cut(s) 65, 556, 776, 918, 1293, 1377
Bst6I CTCTTC 2 cut(s) 980, 1070
BstACI GRCGYC 1 cut(s) 822
BstAFI CTTAAG 2 cut(s) 908, 1268
BstBAI YACGTR 1 cut(s) 1230
BstBI TTCGAA 1 cut(s) 1008
BstC8I GCNNGC 1 cut(s) 1358
BstDEI CTNAG 4 cut(s) 416, 598, 817, 1361
BstDSI CCRYGG 1 cut(s) 201
BstENI CCTNNNNNAGG 1 cut(s) 545
BstF5I GGATG 4 cut(s) 799, 896, 925, 1109
BstFNI CGCG 1 cut(s) 1356
BstHHI GCGC 2 cut(s) 246, 1356
BstKTI GATC 6 cut(s) 178, 337, 640, 679, 685, 754
BstMAI GTCTC 2 cut(s) 115, 545
BstMBI GATC 6 cut(s) 175, 334, 637, 676, 682, 751
BstNI CCWGG 1 cut(s) 110
BstNSI RCATGY 2 cut(s) 308, 466
BstSCI CCNGG 1 cut(s) 108
BstUI CGCG 1 cut(s) 1356
BstV1I GCAGC 2 cut(s) 77, 1457
BstV2I GAAGAC 2 cut(s) 12, 873
BsuRI GGCC 2 cut(s) 906, 932
BtgI CCRYGG 1 cut(s) 201
BtgZI GCGATG 1 cut(s) 1135
BtsCI GGATG 4 cut(s) 799, 896, 925, 1109
BtsIMutI CAGTG 4 cut(s) 61, 424, 660, 844
Cac8I GCNNGC 1 cut(s) 1358
CaiI CAGNNNCTG 1 cut(s) 1451
CciI TCATGA 2 cut(s) 172, 685
CfoI GCGC 2 cut(s) 246, 1356
Cfr13I GGNCC 2 cut(s) 164, 776
CviAII CATG 9 cut(s) 173, 202, 305, 454, 463, 482, 686, 1100, 1492
DdeI CTNAG 4 cut(s) 416, 598, 817, 1361
DpnI GATC 6 cut(s) 177, 336, 639, 678, 684, 753
DpnII GATC 6 cut(s) 175, 334, 637, 676, 682, 751
EaeI YGGCCR 1 cut(s) 930
Eam1104I CTCTTC 2 cut(s) 980, 1070
EarI CTCTTC 2 cut(s) 980, 1070
EciI GGCGGA 1 cut(s) 1225
Eco130I CCWWGG 2 cut(s) 201, 512
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 2 cut(s) 164, 776
Eco57I CTGAAG 1 cut(s) 1051
EcoNI CCTNNNNNAGG 1 cut(s) 545
EcoRI GAATTC 1 cut(s) 1004
EcoRII CCWGG 1 cut(s) 108
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 2 cut(s) 201, 512
EcoT22I ATGCAT 2 cut(s) 927, 1173
ErhI CCWWGG 2 cut(s) 201, 512
FaeI CATG 9 cut(s) 176, 205, 308, 457, 466, 485, 689, 1103, 1495
FalI AAGNNNNNCTT 4 cut(s) 338, 370, 1262, 1294
FaqI GGGAC 1 cut(s) 627
FatI CATG 9 cut(s) 172, 201, 304, 453, 462, 481, 685, 1099, 1491
FauI CCCGC 1 cut(s) 900
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 2 cut(s) 334, 682
Fnu4HI GCNGC 2 cut(s) 91, 1446
FokI GGATG 4 cut(s) 786, 883, 912, 1116
Fsp4HI GCNGC 2 cut(s) 91, 1446
FspBI CTAG 2 cut(s) 656, 1037
FspI TGCGCA 1 cut(s) 245
GlaI GCGC 2 cut(s) 245, 1355
GluI GCNGC 2 cut(s) 91, 1446
HaeIII GGCC 2 cut(s) 906, 932
HapII CCGG 2 cut(s) 929, 975
HhaI GCGC 2 cut(s) 246, 1356
Hin1I GRCGYC 1 cut(s) 822
Hin1II CATG 9 cut(s) 176, 205, 308, 457, 466, 485, 689, 1103, 1495
Hin6I GCGC 2 cut(s) 244, 1354
HinP1I GCGC 2 cut(s) 244, 1354
HincII GTYRAC 1 cut(s) 1053
HindII GTYRAC 1 cut(s) 1053
HindIII AAGCTT 4 cut(s) 56, 407, 1247, 1337
HinfI GANTC 6 cut(s) 68, 502, 954, 971, 1058, 1464
HpaII CCGG 2 cut(s) 929, 975
HphI GGTGA 2 cut(s) 63, 881
Hpy166II GTNNAC 3 cut(s) 188, 1023, 1053
Hpy188I TCNGA 7 cut(s) 101, 405, 601, 804, 820, 946, 965
Hpy188III TCNNGA 5 cut(s) 173, 680, 686, 1069, 1423
Hpy8I GTNNAC 3 cut(s) 188, 1023, 1053
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 6 cut(s) 65, 556, 776, 918, 1293, 1377
HpyCH4IV ACGT 4 cut(s) 266, 822, 1229, 1397
HpyF3I CTNAG 4 cut(s) 416, 598, 817, 1361
HpySE526I ACGT 4 cut(s) 266, 822, 1229, 1397
Hsp92I GRCGYC 1 cut(s) 822
Hsp92II CATG 9 cut(s) 176, 205, 308, 457, 466, 485, 689, 1103, 1495
HspAI GCGC 2 cut(s) 244, 1354
Ksp22I TGATCA 2 cut(s) 334, 682
Kzo9I GATC 6 cut(s) 175, 334, 637, 676, 682, 751
LguI GCTCTTC 1 cut(s) 980
LmnI GCTCC 1 cut(s) 569
Lsp1109I GCAGC 2 cut(s) 77, 1457
LweI GCATC 2 cut(s) 441, 934
MaeI CTAG 2 cut(s) 656, 1037
MaeII ACGT 4 cut(s) 266, 822, 1229, 1397
MaeIII GTNAC 7 cut(s) 154, 230, 830, 839, 1405, 1429, 1465
MalI GATC 6 cut(s) 177, 336, 639, 678, 684, 753
MboI GATC 6 cut(s) 175, 334, 637, 676, 682, 751
MboII GAAGA 7 cut(s) 17, 434, 878, 997, 1022, 1057, 1231
MfeI CAATTG 2 cut(s) 159, 788
MlyI GAGTC 2 cut(s) 963, 1473
MmeI TCCRAC 2 cut(s) 220, 827
MnlI CCTC 7 cut(s) 173, 725, 940, 1157, 1273, 1344, 1430
Mph1103I ATGCAT 2 cut(s) 927, 1173
MroXI GAANNNNTTC 3 cut(s) 353, 399, 970
MseI TTAA 5 cut(s) 269, 282, 618, 909, 1269
MslI CAYNNNNRTG 2 cut(s) 309, 362
MspCI CTTAAG 2 cut(s) 908, 1268
MspI CCGG 2 cut(s) 929, 975
MspR9I CCNGG 1 cut(s) 110
MunI CAATTG 2 cut(s) 159, 788
MvaI CCWGG 1 cut(s) 110
MvnI CGCG 1 cut(s) 1356
NcoI CCATGG 1 cut(s) 201
NdeI CATATG 1 cut(s) 1126
NdeII GATC 6 cut(s) 175, 334, 637, 676, 682, 751
NlaIII CATG 9 cut(s) 176, 205, 308, 457, 466, 485, 689, 1103, 1495
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 4 cut(s) 154, 830, 839, 1465
NsbI TGCGCA 1 cut(s) 245
NsiI ATGCAT 2 cut(s) 927, 1173
NspI RCATGY 2 cut(s) 308, 466
NspV TTCGAA 1 cut(s) 1008
PagI TCATGA 2 cut(s) 172, 685
PciSI GCTCTTC 1 cut(s) 980
PdmI GAANNNNTTC 3 cut(s) 353, 399, 970
PfeI GAWTC 4 cut(s) 68, 502, 971, 1058
PflMI CCANNNNNTGG 1 cut(s) 202
PkrI GCNGC 2 cut(s) 92, 1447
PleI GAGTC 2 cut(s) 962, 1472
PpsI GAGTC 2 cut(s) 962, 1472
Ppu21I YACGTR 1 cut(s) 1230
PshBI ATTAAT 1 cut(s) 282
PsiI TTATAA 2 cut(s) 879, 1254
Psp1406I AACGTT 1 cut(s) 266
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 2 cut(s) 164, 776
PstNI CAGNNNCTG 1 cut(s) 1451
RseI CAYNNNNRTG 2 cut(s) 309, 362
SapI GCTCTTC 1 cut(s) 980
SaqAI TTAA 5 cut(s) 269, 282, 618, 909, 1269
SatI GCNGC 2 cut(s) 91, 1446
Sau3AI GATC 6 cut(s) 175, 334, 637, 676, 682, 751
Sau96I GGNCC 2 cut(s) 164, 776
SchI GAGTC 2 cut(s) 963, 1473
ScrFI CCNGG 1 cut(s) 110
SfaNI GCATC 2 cut(s) 441, 934
SfuI TTCGAA 1 cut(s) 1008
SinI GGWCC 2 cut(s) 164, 776
SmiMI CAYNNNNRTG 2 cut(s) 309, 362
SmlI CTYRAG 3 cut(s) 497, 908, 1268
SmoI CTYRAG 3 cut(s) 497, 908, 1268
SpeI ACTAGT 1 cut(s) 1036
SsiI CCGC 2 cut(s) 893, 1210
SspI AATATT 2 cut(s) 286, 1502
SspMI CTAG 2 cut(s) 656, 1037
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 2 cut(s) 201, 512
TaaI ACNGT 6 cut(s) 65, 556, 776, 918, 1293, 1377
TaiI ACGT 4 cut(s) 269, 825, 1232, 1400
TaqI TCGA 5 cut(s) 178, 291, 393, 1008, 1516
TfiI GAWTC 4 cut(s) 68, 502, 971, 1058
Tru1I TTAA 5 cut(s) 269, 282, 618, 909, 1269
Tru9I TTAA 5 cut(s) 269, 282, 618, 909, 1269
TscAI CASTG 4 cut(s) 68, 424, 667, 844
TseFI GTSAC 4 cut(s) 154, 830, 839, 1465
TseI GCWGC 2 cut(s) 90, 1445
Tsp45I GTSAC 4 cut(s) 154, 830, 839, 1465
TspDTI ATGAA 7 cut(s) 161, 346, 376, 622, 1113, 1323, 1508
TspRI CASTG 4 cut(s) 68, 424, 667, 844
Van91I CCANNNNNTGG 1 cut(s) 202
Vha464I CTTAAG 2 cut(s) 908, 1268
VpaK11BI GGWCC 2 cut(s) 164, 776
VspI ATTAAT 1 cut(s) 282
XagI CCTNNNNNAGG 1 cut(s) 545
XapI RAATTY 3 cut(s) 696, 740, 1004
XceI RCATGY 2 cut(s) 308, 466
XmnI GAANNNNTTC 3 cut(s) 353, 399, 970
XspI CTAG 2 cut(s) 656, 1037
ZraI GACGTC 1 cut(s) 823
Zsp2I ATGCAT 2 cut(s) 927, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.