Rh4DG039300

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0020054
Physical Location & Seq
Forward (+)
6368374 .. 6381404
13031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG039300.1

Sequence Viewer

Length: 435 bp
ATGGAACTCACCGATTCAAGTATCGACAAACTCCGGGACTCCGGAACTGAGCGGACAATGCGGTCCGGACTCGGCATCGATCTCAATGAGAGGATCCCTGGCCCGGCAATCACATTGAAAAGATATCCTGCAAAACTATCACTGACAACATGGATGAGCTTCCTAGGGGCAGCACAGTCAGCTGTCTTTGCAGTTTGTATAGAACATAAACGAGTTGCTTGGACAGTTGGATTCAACATTGACCTCTGGTGCATTTTGTATTCTGGAGGGGTGTGCTCTGGCCTAATATTCTTTATTCAACTATGGTGCACACTAGAGAAAGGACCTGTCTTTGTGACCATGTTTGATCCCGTCTCAACAATATTGGTGGCATTTCTAGCATACTTTGTGCTTGGTGAAAGACTCTACATTGGCAGATTCGTGGTTTCCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

144

Amino Acids

16.01

Weight (kDa)

7.65

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 40 - 140 2.8e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020054)

Species Orthologous Gene IDs
rosa_laevigata RLG00000033566
rosa_multiflora Rmu_sc0003593.1_g000004
rosa_samantha Rh2AG432300 Rh2CG418100 Rh4DG039300 Rh5CG102800 Rh7BG476100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
AccBSI CCGCTC 1 cut(s) 52
AccIII TCCGGA 2 cut(s) 41, 65
AciI CCGC 3 cut(s) 52, 61, 429
AclWI GGATC 3 cut(s) 88, 101, 341
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 4 cut(s) 18, 118, 235, 299
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 2 cut(s) 159, 182
AluI AGCT 2 cut(s) 159, 182
Alw21I GWGCWC 2 cut(s) 278, 311
Alw26I GTCTC 1 cut(s) 358
Alw44I GTGCAC 1 cut(s) 307
AlwI GGATC 3 cut(s) 88, 101, 341
Aor13HI TCCGGA 2 cut(s) 41, 65
AoxI GGCC 2 cut(s) 100, 280
ApaLI GTGCAC 1 cut(s) 307
ApeKI GCWGC 1 cut(s) 170
AspA2I CCTAGG 1 cut(s) 163
AspS9I GGNCC 3 cut(s) 63, 101, 323
AsuC2I CCSGG 2 cut(s) 35, 104
AsuHPI GGTGA 1 cut(s) 407
AvaII GGWCC 2 cut(s) 63, 323
AvrII CCTAGG 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 311
BamHI GGATCC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 278, 311
BbvI GCAGC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 99
BcnI CCSGG 2 cut(s) 35, 104
BcoDI GTCTC 1 cut(s) 358
BfaI CTAG 3 cut(s) 164, 314, 377
BisI GCNGC 1 cut(s) 171
BlnI CCTAGG 1 cut(s) 163
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 3 cut(s) 35, 99, 104
Bme18I GGWCC 2 cut(s) 63, 323
BmgT120I GGNCC 3 cut(s) 63, 101, 323
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 3 cut(s) 35, 99, 104
BmsI GCATC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 285
BpuMI CCSGG 2 cut(s) 35, 104
Bsa29I ATCGAT 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 97, 163
BsaWI WCCGGW 2 cut(s) 41, 65
Bsc4I CCNNNNNNNGG 1 cut(s) 103
BseAI TCCGGA 2 cut(s) 41, 65
BseBI CCWGG 1 cut(s) 99
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 97, 163
BseGI GGATG 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 39
BseSI GKGCMC 1 cut(s) 311
BseXI GCAGC 1 cut(s) 182
BshFI GGCC 2 cut(s) 102, 282
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 278, 311
BsiSI CCGG 4 cut(s) 34, 42, 66, 104
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 358
BsmBI CGTCTC 1 cut(s) 358
BsmFI GGGAC 1 cut(s) 50
BsnI GGCC 2 cut(s) 102, 282
Bsp1286I GDGCHC 2 cut(s) 278, 311
Bsp13I TCCGGA 2 cut(s) 41, 65
Bsp143I GATC 3 cut(s) 79, 93, 346
BspACI CCGC 3 cut(s) 52, 61, 429
BspANI GGCC 2 cut(s) 102, 282
BspCNI CTCAG 1 cut(s) 40
BspDI ATCGAT 1 cut(s) 78
BspEI TCCGGA 2 cut(s) 41, 65
BspLI GGNNCC 1 cut(s) 95
BspPI GGATC 3 cut(s) 88, 101, 341
BsrBI CCGCTC 1 cut(s) 52
BssECI CCNNGG 2 cut(s) 97, 163
BssMI GATC 3 cut(s) 79, 93, 346
BssT1I CCWWGG 1 cut(s) 163
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 2 cut(s) 177, 226
BstDEI CTNAG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 159
BstKTI GATC 3 cut(s) 82, 96, 349
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 3 cut(s) 79, 93, 346
BstMWI GCNNNNNNNGC 4 cut(s) 58, 179, 188, 377
BstNI CCWGG 1 cut(s) 99
BstSCI CCNGG 3 cut(s) 33, 97, 102
BstSLI GKGCMC 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 78
BsuRI GGCC 2 cut(s) 102, 282
BsuTUI ATCGAT 1 cut(s) 78
BtsCI GGATG 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 63, 101, 323
ClaI ATCGAT 1 cut(s) 78
CpoI CGGWCCG 1 cut(s) 63
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CspI CGGWCCG 1 cut(s) 63
CviAII CATG 2 cut(s) 150, 340
CviJI RGCY 4 cut(s) 102, 159, 182, 282
CviKI_1 RGCY 4 cut(s) 102, 159, 182, 282
DdeI CTNAG 1 cut(s) 48
DpnI GATC 3 cut(s) 81, 95, 348
DpnII GATC 3 cut(s) 79, 93, 346
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 163
Eco32I GATATC 1 cut(s) 125
Eco47I GGWCC 2 cut(s) 63, 323
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 97
EcoRV GATATC 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 163
Esp3I CGTCTC 1 cut(s) 358
FaeI CATG 2 cut(s) 153, 343
FaiI YATR 7 cut(s) 151, 200, 207, 304, 341, 382, 433
FaqI GGGAC 1 cut(s) 50
FatI CATG 2 cut(s) 149, 339
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 3 cut(s) 164, 314, 377
GluI GCNGC 1 cut(s) 171
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 2 cut(s) 102, 282
HapII CCGG 4 cut(s) 34, 42, 66, 104
Hin1II CATG 2 cut(s) 153, 343
HinfI GANTC 6 cut(s) 14, 38, 69, 231, 402, 417
HpaII CCGG 4 cut(s) 34, 42, 66, 104
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 1 cut(s) 309
Hpy188III TCNNGA 3 cut(s) 42, 66, 264
Hpy8I GTNNAC 1 cut(s) 309
HpyCH4III ACNGT 2 cut(s) 177, 226
HpyCH4V TGCA 4 cut(s) 131, 191, 252, 309
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 179, 188, 377
HpyF3I CTNAG 1 cut(s) 48
Hsp92II CATG 2 cut(s) 153, 343
Kpn2I TCCGGA 2 cut(s) 41, 65
Kzo9I GATC 3 cut(s) 79, 93, 346
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 84
MaeI CTAG 3 cut(s) 164, 314, 377
MaeIII GTNAC 1 cut(s) 334
MalI GATC 3 cut(s) 81, 95, 348
MbiI CCGCTC 1 cut(s) 52
MboI GATC 3 cut(s) 79, 93, 346
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 278, 311
MlyI GAGTC 3 cut(s) 32, 63, 396
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 3 cut(s) 84, 254, 260
MroI TCCGGA 2 cut(s) 41, 65
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 4 cut(s) 34, 42, 66, 104
MspR9I CCNGG 3 cut(s) 35, 99, 104
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 4 cut(s) 58, 179, 188, 377
NciI CCSGG 2 cut(s) 35, 104
NdeII GATC 3 cut(s) 79, 93, 346
NlaIII CATG 2 cut(s) 153, 343
NlaIV GGNNCC 1 cut(s) 95
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 334
PfeI GAWTC 3 cut(s) 14, 231, 417
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 3 cut(s) 32, 63, 396
PpsI GAGTC 3 cut(s) 32, 63, 396
PpuMI RGGWCCY 1 cut(s) 323
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 3 cut(s) 63, 101, 323
PspPPI RGGWCCY 1 cut(s) 323
PsuI RGATCY 1 cut(s) 93
PvuII CAGCTG 1 cut(s) 182
Rsr2I CGGWCCG 1 cut(s) 63
RsrII CGGWCCG 1 cut(s) 63
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 3 cut(s) 79, 93, 346
Sau96I GGNCC 3 cut(s) 63, 101, 323
SchI GAGTC 3 cut(s) 32, 63, 396
ScrFI CCNGG 3 cut(s) 35, 99, 104
SduI GDGCHC 2 cut(s) 278, 311
SetI ASST 4 cut(s) 161, 184, 246, 328
SfaNI GCATC 1 cut(s) 84
SinI GGWCC 2 cut(s) 63, 323
SsiI CCGC 3 cut(s) 52, 61, 429
SspI AATATT 2 cut(s) 288, 363
SspMI CTAG 3 cut(s) 164, 314, 377
StyD4I CCNGG 3 cut(s) 33, 97, 102
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 2 cut(s) 177, 226
TaqI TCGA 2 cut(s) 24, 78
TfiI GAWTC 3 cut(s) 14, 231, 417
TscAI CASTG 1 cut(s) 147
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 334
TspRI CASTG 1 cut(s) 147
VneI GTGCAC 1 cut(s) 307
VpaK11BI GGWCC 2 cut(s) 63, 323
XmaJI CCTAGG 1 cut(s) 163
XspI CTAG 3 cut(s) 164, 314, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.