Rh5AG299100

Nucleolar complex protein 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006353
Physical Location & Seq
Forward (+)
41787356 .. 41794014
6659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG299100.1

Sequence Viewer

Length: 1935 bp
ATGGGGAAACTGGGGAAGAAGGCCAGGAAGTTTTCCAAGAAGAATCTTCAGTCTGTCGAGAGAAGAAACCGCAAGTTGAAGTCCACCTTCAAAAAGAGAGGTCCCAAGAGAAATGAGCAAGGTGGTGGTGGAGAATTGAAGAAGAAGGACGCGGTAGAGTTGTCTAATGGGAGAAACACTGAAGTTGAATACATAGACGACACTCCTCTTGACGCAATATTCCATGAAGATGACAGTGATGCGTTTGGAGATGAATCGGACAGTGATGGGTATCTTTCAGAGGACTCAAGTGAAATGCATCTTGCTGACAGTGAAATTGAGAATTCTCAAGAAGGCAACCTTGATGGTAGTAGTGGTGCTTTATCTATTCAGAACGAGGAAATTCAATTAGAGCTTGTGAAGAAAACAAAAAAACTAGACAAATTGAAGGAGATGGATCCTGAATTTGGCAACTTTTTGGAAAGCTATCATAAGGAGAGCGAACAGTTCAGAAAGAAAGATTATGCTGATGAGGATGAGGATGAGGATGAGGATGAGATGAGTGATGATAATATGCAGCCAGAGAATGTAGACTGTGTGAATTTTAATTGGGGCAACCTGCTGACAAGCTCTTATGTTGATTCCTTGTGTCAACTAGTCACAGAGCAACAAAATGTGTCTGCACTTACTAGTCTATTGAATGGGTACCGGGCTGCATGCCACTATGGAGCAGAATCAACTAGAGTATTTGATGCTTATTCAGGCCGTGGAATTCAGAATAGTGAAACCCTTAGCAAGATATTGATGTTCATGCTTAATGAAGCTGATAACATATTTAGGGGGCTAATTGGCCTACCAAGCTTGGATTCCAAAAAAGAGAAGAGTGTGGATTTGAAGAAGAATACCAAATGGAGTACTTTTAAGCCACTGATAAAGTCATATCTGAGGAGTACCCTTTTTCTCCTGAATCAGGTTGATGACTCTGAGATATTGGCCTTCTCCTTGGCACGGATCAGAGCTTCTATAACCTTTTTTAATGCTTTTCCTTCTTTGCTCCGCAGACTTATCAAGATTGCCGTTCACTTGTGGGCAACAGGGAGAGGAACTGTATCATCTCTATCCTTTCTCATCATACGAGATGTGGCTTCTGTGTTTCGCTCTGATTACTTCGACACCTGCTTTGTTAAGACATATAAATCGTTCCTTGGTCATTGCCAATTTGTGGAGCCTGGTCTGTTCCAACACATACAATTTTTAAGAAGTTCCATTATTGATCTATGCTCTGTCGATGTTCAGAAAGCATCTAGCAAAGCATTGGTTTGTATCCAGCAACTTTCTAAGATAATGCAGCAGGGTCTACGGACAAAGAAAAAGGAAGCAGTCAAGAAGATATGCAGTTGGCAATACACGAGTTGCATTGATCTCTGGGTAATGTTTATATCAGCTAACATACAGGATTATGATCTTCAGCCATCGCTTTTTATGGTCATTCAGATTATAAATGGAGTGGCTGTGTTGTTTTCTGGACCAAGATATTTGCCTTTGAGAATCAATTGCATTCAATGGTTGAATCGTCTCTCCTCTTCCAGTGGCATTTTCATTCCTGTTGCCTCTTTCGTGTTGGATATCTTAGAATATAAAATTAGCAAGGATGGCGCGAAACCTGGAAAAGCTTTCAACCATTTATCTTCCGTAAAGCTTCCAAAGCATTGGTTAAAGTCAAGGAACTTCCAAGAGCAGTGTGTTTTATCTACTATCGAACTGCTTTCAGCGCACTTCGCTCAGTGGAGCCATCATATATCGTTTCCAGATCTTGCAACTATTCCACTTATTTGCTTGAAGAAGTTTCATGAGATAACTACTATAGAAAGTTCCAAGCGTGTAGTGAAGCGTTTCATTGACCAGGTAAATTTTTCATGGAACAATTTATTGTTTATTTGCAATTTCCTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Noc2

Protein Analysis

644

Amino Acids

73.31

Weight (kDa)

6.9

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Noc2 PF03715 382 - 630 2.7e-72 Noc2p family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1478
AarI CACCTGC 1 cut(s) 1163
Acc36I ACCTGC 2 cut(s) 606, 1163
Acc65I GGTACC 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 684
AccB7I CCANNNNNTGG 1 cut(s) 1201
AccI GTMKAC 2 cut(s) 570, 1336
AccII CGCG 2 cut(s) 152, 1637
AciI CCGC 3 cut(s) 70, 152, 1036
AclWI GGATC 3 cut(s) 431, 444, 998
AcsI RAATTY 6 cut(s) 322, 381, 443, 580, 750, 1888
AcuI CTGAAG 3 cut(s) 32, 201, 1430
AfaI GTAC 3 cut(s) 686, 895, 931
AfiI CCNNNNNNNGG 4 cut(s) 446, 949, 987, 1201
AhlI ACTAGT 2 cut(s) 634, 668
AjnI CCWGG 4 cut(s) 23, 1207, 1642, 1881
AjuI GAANNNNNNNTTGG 2 cut(s) 829, 861
AluBI AGCT 9 cut(s) 394, 465, 609, 803, 840, 998, 1424, 1652, 1678
AluI AGCT 9 cut(s) 394, 465, 609, 803, 840, 998, 1424, 1652, 1678
Alw26I GTCTC 1 cut(s) 1559
AlwI GGATC 3 cut(s) 431, 444, 998
AoxI GGCC 4 cut(s) 21, 742, 829, 972
ApeKI GCWGC 3 cut(s) 556, 692, 1327
ApoI RAATTY 6 cut(s) 322, 381, 443, 580, 750, 1888
ArsI GACNNNNNNTTYG 2 cut(s) 1142, 1174
Asp700I GAANNNNTTC 1 cut(s) 1871
Asp718I GGTACC 1 cut(s) 684
AspLEI GCGC 2 cut(s) 1637, 1753
AspS9I GGNCC 2 cut(s) 101, 1505
AsuC2I CCSGG 1 cut(s) 689
AvaII GGWCC 2 cut(s) 101, 1505
BamHI GGATCC 1 cut(s) 436
BanI GGYRCC 1 cut(s) 684
BauI CACGAG 1 cut(s) 1387
BbvI GCAGC 3 cut(s) 568, 679, 1339
BccI CCATC 6 cut(s) 260, 338, 427, 1459, 1625, 1779
BceAI ACGGC 2 cut(s) 729, 1040
BciT130I CCWGG 4 cut(s) 25, 1209, 1644, 1883
BciVI GTATCC 1 cut(s) 1313
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 1 cut(s) 1559
BcuI ACTAGT 2 cut(s) 634, 668
BfaI CTAG 5 cut(s) 416, 635, 669, 720, 1284
BfmI CTRYAG 1 cut(s) 1842
BfuAI ACCTGC 2 cut(s) 606, 1163
BfuI GTATCC 1 cut(s) 1313
BglII AGATCT 1 cut(s) 1789
BisI GCNGC 3 cut(s) 557, 693, 1328
BlsI GCNGC 3 cut(s) 558, 694, 1329
BmcAI AGTACT 1 cut(s) 895
Bme1390I CCNGG 5 cut(s) 25, 689, 1209, 1644, 1883
Bme18I GGWCC 2 cut(s) 101, 1505
BmgT120I GGNCC 2 cut(s) 101, 1505
BmiI GGNNCC 5 cut(s) 103, 438, 686, 1206, 1769
BmrFI CCNGG 5 cut(s) 25, 689, 1209, 1644, 1883
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 4 cut(s) 229, 307, 721, 1289
BmuI ACTGGG 1 cut(s) 20
Bpu10I CCTNAGC 1 cut(s) 770
BpuEI CTTGAG 2 cut(s) 271, 312
BpuMI CCSGG 1 cut(s) 689
BsaBI GATNNNNATC 2 cut(s) 270, 1440
BsaJI CCNNGG 3 cut(s) 745, 981, 1183
BsaXI ACNNNNNCTCC 2 cut(s) 1476, 1506
Bsc4I CCNNNNNNNGG 4 cut(s) 446, 949, 987, 1201
Bse1I ACTGG 2 cut(s) 15, 1566
Bse3DI GCAATG 1 cut(s) 1189
Bse8I GATNNNNATC 2 cut(s) 270, 1440
BseBI CCWGG 4 cut(s) 25, 1209, 1644, 1883
BseDI CCNNGG 3 cut(s) 745, 981, 1183
BseGI GGATG 5 cut(s) 520, 526, 532, 538, 1636
BseJI GATNNNNATC 2 cut(s) 270, 1440
BseLI CCNNNNNNNGG 4 cut(s) 446, 949, 987, 1201
BseMI GCAATG 1 cut(s) 1189
BseMII CTCAG 3 cut(s) 914, 954, 1775
BseNI ACTGG 2 cut(s) 15, 1566
BseRI GAGGAG 3 cut(s) 195, 940, 1549
BseXI GCAGC 3 cut(s) 568, 679, 1339
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 2 cut(s) 152, 1637
BshFI GGCC 4 cut(s) 23, 744, 831, 974
BshNI GGYRCC 1 cut(s) 684
BsiSI CCGG 1 cut(s) 688
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 4 cut(s) 446, 949, 987, 1201
BsmAI GTCTC 1 cut(s) 1559
BsmBI CGTCTC 1 cut(s) 1559
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 1 cut(s) 1536
BsnI GGCC 4 cut(s) 23, 744, 831, 974
Bsp143I GATC 6 cut(s) 436, 990, 1252, 1399, 1441, 1789
BspACI CCGC 3 cut(s) 70, 152, 1036
BspANI GGCC 4 cut(s) 23, 744, 831, 974
BspCNI CTCAG 3 cut(s) 915, 955, 1774
BspFNI CGCG 2 cut(s) 152, 1637
BspHI TCATGA 1 cut(s) 1828
BspLI GGNNCC 5 cut(s) 103, 438, 686, 1206, 1769
BspMI ACCTGC 2 cut(s) 606, 1163
BspPI GGATC 3 cut(s) 431, 444, 998
BspT107I GGYRCC 1 cut(s) 684
BsrDI GCAATG 1 cut(s) 1189
BsrI ACTGG 2 cut(s) 15, 1566
BssECI CCNNGG 3 cut(s) 745, 981, 1183
BssMI GATC 6 cut(s) 436, 990, 1252, 1399, 1441, 1789
BssSI CACGAG 1 cut(s) 1387
BssT1I CCWWGG 2 cut(s) 981, 1183
Bst2BI CACGAG 1 cut(s) 1387
Bst2UI CCWGG 4 cut(s) 25, 1209, 1644, 1883
Bst4CI ACNGT 6 cut(s) 236, 263, 311, 486, 575, 1087
Bst6I CTCTTC 2 cut(s) 854, 1567
BstC8I GCNNGC 1 cut(s) 697
BstDEI CTNAG 6 cut(s) 770, 923, 963, 1317, 1609, 1761
BstDSI CCRYGG 1 cut(s) 745
BstENI CCTNNNNNAGG 1 cut(s) 947
BstF5I GGATG 5 cut(s) 520, 526, 532, 538, 1636
BstFNI CGCG 2 cut(s) 152, 1637
BstHHI GCGC 2 cut(s) 1637, 1753
BstKTI GATC 6 cut(s) 439, 993, 1255, 1402, 1444, 1792
BstMAI GTCTC 1 cut(s) 1559
BstMBI GATC 6 cut(s) 436, 990, 1252, 1399, 1441, 1789
BstMWI GCNNNNNNNGC 5 cut(s) 837, 1632, 1684, 1750, 1757
BstNI CCWGG 4 cut(s) 25, 1209, 1644, 1883
BstNSI RCATGY 1 cut(s) 699
BstSCI CCNGG 5 cut(s) 23, 687, 1207, 1642, 1881
BstSFI CTRYAG 1 cut(s) 1842
BstUI CGCG 2 cut(s) 152, 1637
BstV1I GCAGC 3 cut(s) 568, 679, 1339
BstX2I RGATCY 2 cut(s) 436, 1789
BstXI CCANNNNNNTGG 1 cut(s) 1689
BstYI RGATCY 2 cut(s) 436, 1789
BsuI GTATCC 1 cut(s) 1313
BsuRI GGCC 4 cut(s) 23, 744, 831, 974
BtgI CCRYGG 1 cut(s) 745
BtgZI GCGATG 1 cut(s) 1437
BtsCI GGATG 5 cut(s) 520, 526, 532, 538, 1636
BtsI GCAGTG 1 cut(s) 1724
BtsIMutI CAGTG 8 cut(s) 177, 241, 268, 316, 905, 1573, 1724, 1769
BveI ACCTGC 2 cut(s) 606, 1163
Cac8I GCNNGC 1 cut(s) 697
CciI TCATGA 1 cut(s) 1828
CfoI GCGC 2 cut(s) 1637, 1753
Cfr13I GGNCC 2 cut(s) 101, 1505
CseI GACGC 2 cut(s) 158, 221
CsiI ACCWGGT 1 cut(s) 1881
Csp6I GTAC 3 cut(s) 685, 894, 930
CviAII CATG 5 cut(s) 224, 696, 790, 1829, 1896
CviQI GTAC 3 cut(s) 685, 894, 930
DdeI CTNAG 6 cut(s) 770, 923, 963, 1317, 1609, 1761
DpnI GATC 6 cut(s) 438, 992, 1254, 1401, 1443, 1791
DpnII GATC 6 cut(s) 436, 990, 1252, 1399, 1441, 1789
Eam1104I CTCTTC 2 cut(s) 854, 1567
EarI CTCTTC 2 cut(s) 854, 1567
Eco130I CCWWGG 2 cut(s) 981, 1183
Eco32I GATATC 1 cut(s) 1606
Eco47I GGWCC 2 cut(s) 101, 1505
Eco57I CTGAAG 3 cut(s) 32, 201, 1430
EcoNI CCTNNNNNAGG 1 cut(s) 947
EcoO109I RGGNCCY 1 cut(s) 101
EcoRI GAATTC 2 cut(s) 322, 750
EcoRII CCWGG 4 cut(s) 23, 1207, 1642, 1881
EcoRV GATATC 1 cut(s) 1606
EcoT14I CCWWGG 2 cut(s) 981, 1183
EcoT22I ATGCAT 1 cut(s) 300
ErhI CCWWGG 2 cut(s) 981, 1183
Esp3I CGTCTC 1 cut(s) 1559
FaeI CATG 5 cut(s) 227, 699, 793, 1832, 1899
FalI AAGNNNNNCTT 4 cut(s) 71, 103, 324, 356
FaqI GGGAC 1 cut(s) 87
FatI CATG 5 cut(s) 223, 695, 789, 1828, 1895
FblI GTMKAC 2 cut(s) 570, 1336
Fnu4HI GCNGC 3 cut(s) 557, 693, 1328
FokI GGATG 5 cut(s) 527, 533, 539, 545, 1643
Fsp4HI GCNGC 3 cut(s) 557, 693, 1328
FspBI CTAG 5 cut(s) 416, 635, 669, 720, 1284
GlaI GCGC 2 cut(s) 1636, 1752
GluI GCNGC 3 cut(s) 557, 693, 1328
HaeIII GGCC 4 cut(s) 23, 744, 831, 974
HapII CCGG 1 cut(s) 688
HgaI GACGC 2 cut(s) 158, 221
HhaI GCGC 2 cut(s) 1637, 1753
Hin1II CATG 5 cut(s) 227, 699, 793, 1832, 1899
Hin6I GCGC 2 cut(s) 1635, 1751
HinP1I GCGC 2 cut(s) 1635, 1751
HincII GTYRAC 1 cut(s) 632
HindII GTYRAC 1 cut(s) 632
HindIII AAGCTT 3 cut(s) 838, 1650, 1676
HpaII CCGG 1 cut(s) 688
Hpy166II GTNNAC 5 cut(s) 84, 571, 632, 1060, 1337
Hpy8I GTNNAC 5 cut(s) 84, 571, 632, 1060, 1337
HpyAV CCTTC 7 cut(s) 13, 97, 139, 326, 421, 985, 1035
HpyCH4III ACNGT 6 cut(s) 236, 263, 311, 486, 575, 1087
HpyF10VI GCNNNNNNNGC 5 cut(s) 837, 1632, 1684, 1750, 1757
HpyF3I CTNAG 6 cut(s) 770, 923, 963, 1317, 1609, 1761
Hsp92II CATG 5 cut(s) 227, 699, 793, 1832, 1899
HspAI GCGC 2 cut(s) 1635, 1751
KpnI GGTACC 1 cut(s) 688
Kzo9I GATC 6 cut(s) 436, 990, 1252, 1399, 1441, 1789
LmnI GCTCC 4 cut(s) 707, 1038, 1204, 1767
Lsp1109I GCAGC 3 cut(s) 568, 679, 1339
LweI GCATC 4 cut(s) 229, 307, 721, 1289
MabI ACCWGGT 1 cut(s) 1881
MaeI CTAG 5 cut(s) 416, 635, 669, 720, 1284
MaeIII GTNAC 1 cut(s) 637
MalI GATC 6 cut(s) 438, 992, 1254, 1401, 1443, 1791
MboI GATC 6 cut(s) 436, 990, 1252, 1399, 1441, 1789
MfeI CAATTG 1 cut(s) 1531
MflI RGATCY 2 cut(s) 436, 1789
MlyI GAGTC 2 cut(s) 278, 953
MmeI TCCRAC 2 cut(s) 1243, 1581
Mph1103I ATGCAT 1 cut(s) 300
MroXI GAANNNNTTC 1 cut(s) 1871
MseI TTAA 7 cut(s) 585, 795, 900, 1014, 1164, 1235, 1694
MslI CAYNNNNRTG 1 cut(s) 228
MspI CCGG 1 cut(s) 688
MspR9I CCNGG 5 cut(s) 25, 689, 1209, 1644, 1883
MunI CAATTG 1 cut(s) 1531
Mva1269I GAATGC 1 cut(s) 1536
MvaI CCWGG 4 cut(s) 25, 1209, 1644, 1883
MvnI CGCG 2 cut(s) 152, 1637
MwoI GCNNNNNNNGC 5 cut(s) 837, 1632, 1684, 1750, 1757
NciI CCSGG 1 cut(s) 689
NdeII GATC 6 cut(s) 436, 990, 1252, 1399, 1441, 1789
NlaIII CATG 5 cut(s) 227, 699, 793, 1832, 1899
NlaIV GGNNCC 5 cut(s) 103, 438, 686, 1206, 1769
NmuCI GTSAC 1 cut(s) 637
NsiI ATGCAT 1 cut(s) 300
NspI RCATGY 1 cut(s) 699
PaeI GCATGC 1 cut(s) 699
PagI TCATGA 1 cut(s) 1828
PaqCI CACCTGC 1 cut(s) 1163
PctI GAATGC 1 cut(s) 1536
PdmI GAANNNNTTC 1 cut(s) 1871
PfeI GAWTC 8 cut(s) 43, 254, 620, 713, 845, 946, 1527, 1549
PflMI CCANNNNNTGG 1 cut(s) 1201
PkrI GCNGC 3 cut(s) 558, 694, 1329
PleI GAGTC 2 cut(s) 278, 953
PpsI GAGTC 2 cut(s) 278, 953
PpuMI RGGWCCY 1 cut(s) 101
PsiI TTATAA 1 cut(s) 1478
Psp5II RGGWCCY 1 cut(s) 101
Psp6I CCWGG 4 cut(s) 23, 1207, 1642, 1881
PspGI CCWGG 4 cut(s) 23, 1207, 1642, 1881
PspN4I GGNNCC 5 cut(s) 103, 438, 686, 1206, 1769
PspPI GGNCC 2 cut(s) 101, 1505
PspPPI RGGWCCY 1 cut(s) 101
PsuI RGATCY 2 cut(s) 436, 1789
RsaI GTAC 3 cut(s) 686, 895, 931
RsaNI GTAC 3 cut(s) 685, 894, 930
RseI CAYNNNNRTG 1 cut(s) 228
SaqAI TTAA 7 cut(s) 585, 795, 900, 1014, 1164, 1235, 1694
SatI GCNGC 3 cut(s) 557, 693, 1328
Sau3AI GATC 6 cut(s) 436, 990, 1252, 1399, 1441, 1789
Sau96I GGNCC 2 cut(s) 101, 1505
ScaI AGTACT 1 cut(s) 895
SchI GAGTC 2 cut(s) 278, 953
ScrFI CCNGG 5 cut(s) 25, 689, 1209, 1644, 1883
SexAI ACCWGGT 1 cut(s) 1881
SfaNI GCATC 4 cut(s) 229, 307, 721, 1289
SfcI CTRYAG 1 cut(s) 1842
SinI GGWCC 2 cut(s) 101, 1505
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 2 cut(s) 286, 327
SmoI CTYRAG 2 cut(s) 286, 327
SpeI ACTAGT 2 cut(s) 634, 668
SphI GCATGC 1 cut(s) 699
SsiI CCGC 3 cut(s) 70, 152, 1036
SspI AATATT 1 cut(s) 219
SspMI CTAG 5 cut(s) 416, 635, 669, 720, 1284
StyD4I CCNGG 5 cut(s) 23, 687, 1207, 1642, 1881
StyI CCWWGG 2 cut(s) 981, 1183
TaaI ACNGT 6 cut(s) 236, 263, 311, 486, 575, 1087
TaqI TCGA 4 cut(s) 57, 1149, 1266, 1737
TatI WGTACW 1 cut(s) 893
TfiI GAWTC 8 cut(s) 43, 254, 620, 713, 845, 946, 1527, 1549
Tru1I TTAA 7 cut(s) 585, 795, 900, 1014, 1164, 1235, 1694
Tru9I TTAA 7 cut(s) 585, 795, 900, 1014, 1164, 1235, 1694
TscAI CASTG 8 cut(s) 184, 241, 268, 316, 912, 1573, 1724, 1769
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 3 cut(s) 556, 692, 1327
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 8 cut(s) 240, 267, 778, 813, 1567, 1817, 1864, 1884
TspGWI ACGGA 3 cut(s) 1003, 1354, 1660
TspRI CASTG 8 cut(s) 184, 241, 268, 316, 912, 1573, 1724, 1769
Van91I CCANNNNNTGG 1 cut(s) 1201
VpaK11BI GGWCC 2 cut(s) 101, 1505
XagI CCTNNNNNAGG 1 cut(s) 947
XapI RAATTY 6 cut(s) 322, 381, 443, 580, 750, 1888
XceI RCATGY 1 cut(s) 699
XmiI GTMKAC 2 cut(s) 570, 1336
XmnI GAANNNNTTC 1 cut(s) 1871
XspI CTAG 5 cut(s) 416, 635, 669, 720, 1284
ZrmI AGTACT 1 cut(s) 895
Zsp2I ATGCAT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.