Rh5AG464300

No apical meristem-associated C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0019548
Physical Location & Seq
Reverse (-)
79441013 .. 79451703
10691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG464300.1

Sequence Viewer

Length: 495 bp
ATGATTCAATCTGGGTCTTCACTGAGGTTGTCTGTTTGGGTTCGGTTGCCGGCGATTTGTTCAGATGGCAGTAGAGGCGGAGCCGTAGAGAGAAAGAATGGGGTCGGAGGGGTGGTAGTGATGATTGGAGAGACACTAAGGTTGAGGCGCCCAGATACAATAGATTTAGCTGATGGCAATGTTGAAGGCAATGAGTCTCCATGCTTGGAGAGGCCTATAGGCAAAAAGGCTGCCAAGACCTTGGCAAGGAAGGCAAAAGCTAAAGAGAAAGTGGAAGCAGCCCAATCTGAATTAGGAGACTATTATGCTCTGAAAGTTGAACATGCTCATAAGGAGGATGAGCAATTTCAACTGATGTATGATGCAGAACAAGAGAACATTAAGCTAAGGAAACAAGAACTTGAAATTCAAGTTCGAAAAGAGGATAATGCAATAATGGCAATTGATACTTCTACAATGGAACCAATGCGGGCAGAATATTTTAGAGGACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM-associated

Protein Analysis

164

Amino Acids

18.29

Weight (kDa)

6.2

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 63 - 162 2e-09 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019548)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g16231 FvH4_3g26413 FvH4_5g21960
rosa_laevigata RLG00000024698
rosa_roxburghii Rroxscaffold_2G00090990
rosa_samantha Rh5AG464300 Rh5BG276700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 147
AciI CCGC 2 cut(s) 78, 469
AcsI RAATTY 1 cut(s) 405
AcyI GRCGYC 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 246
AgsI TTSAA 6 cut(s) 8, 185, 320, 350, 404, 410
AluBI AGCT 3 cut(s) 170, 260, 385
AluI AGCT 3 cut(s) 170, 260, 385
Alw26I GTCTC 3 cut(s) 125, 201, 291
AoxI GGCC 1 cut(s) 212
ApeKI GCWGC 2 cut(s) 230, 278
ApoI RAATTY 1 cut(s) 405
AspLEI GCGC 1 cut(s) 150
AsuII TTCGAA 1 cut(s) 415
BanI GGYRCC 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 217, 290
BccI CCATC 2 cut(s) 59, 167
BceAI ACGGC 1 cut(s) 68
BcoDI GTCTC 3 cut(s) 125, 201, 291
BfmI CTRYAG 1 cut(s) 216
BfoI RGCGCY 1 cut(s) 151
BisI GCNGC 2 cut(s) 231, 279
BlsI GCNGC 2 cut(s) 232, 280
BmiI GGNNCC 3 cut(s) 82, 149, 462
BmsI GCATC 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 9
Bpu10I CCTNAGC 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 415
BsaHI GRCGYC 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 1 cut(s) 246
Bse118I RCCGGY 1 cut(s) 49
Bse3DI GCAATG 2 cut(s) 184, 196
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 1 cut(s) 246
BseMI GCAATG 2 cut(s) 184, 196
BseMII CTCAG 1 cut(s) 14
BseXI GCAGC 2 cut(s) 217, 290
BshFI GGCC 1 cut(s) 214
BshNI GGYRCC 1 cut(s) 147
BsiSI CCGG 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 246
BsmAI GTCTC 3 cut(s) 125, 201, 291
BsnI GGCC 1 cut(s) 214
Bsp119I TTCGAA 1 cut(s) 415
BspACI CCGC 2 cut(s) 78, 469
BspANI GGCC 1 cut(s) 214
BspCNI CTCAG 1 cut(s) 15
BspLI GGNNCC 3 cut(s) 82, 149, 462
BspT104I TTCGAA 1 cut(s) 415
BspT107I GGYRCC 1 cut(s) 147
BsrDI GCAATG 2 cut(s) 184, 196
BsrFI RCCGGY 1 cut(s) 49
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 240
BssNI GRCGYC 1 cut(s) 148
BssT1I CCWWGG 1 cut(s) 240
BstACI GRCGYC 1 cut(s) 148
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 51, 471
BstDEI CTNAG 3 cut(s) 23, 137, 386
BstENI CCTNNNNNAGG 1 cut(s) 244
BstF5I GGATG 1 cut(s) 343
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 1 cut(s) 150
BstMAI GTCTC 3 cut(s) 125, 201, 291
BstMWI GCNNNNNNNGC 3 cut(s) 75, 251, 437
BstNSI RCATGY 1 cut(s) 326
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 2 cut(s) 217, 290
BstV2I GAAGAC 1 cut(s) 9
BstXI CCANNNNNNTGG 1 cut(s) 241
BsuRI GGCC 1 cut(s) 214
BtsCI GGATG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 20
Cac8I GCNNGC 2 cut(s) 51, 471
CfoI GCGC 1 cut(s) 150
Cfr10I RCCGGY 1 cut(s) 49
CviAII CATG 2 cut(s) 201, 323
CviJI RGCY 7 cut(s) 83, 170, 214, 230, 260, 281, 385
CviKI_1 RGCY 7 cut(s) 83, 170, 214, 230, 260, 281, 385
DdeI CTNAG 3 cut(s) 23, 137, 386
DinI GGCGCC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 240
Eco147I AGGCCT 1 cut(s) 214
EcoNI CCTNNNNNAGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 240
EgeI GGCGCC 1 cut(s) 149
EheI GGCGCC 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 2 cut(s) 204, 326
FaiI YATR 6 cut(s) 202, 218, 306, 324, 330, 360
FatI CATG 2 cut(s) 200, 322
FauI CCCGC 1 cut(s) 462
Fnu4HI GCNGC 2 cut(s) 231, 279
FokI GGATG 1 cut(s) 350
Fsp4HI GCNGC 2 cut(s) 231, 279
GlaI GCGC 1 cut(s) 149
GluI GCNGC 2 cut(s) 231, 279
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 1 cut(s) 214
HapII CCGG 1 cut(s) 50
HhaI GCGC 1 cut(s) 150
Hin1I GRCGYC 1 cut(s) 148
Hin1II CATG 2 cut(s) 204, 326
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HinfI GANTC 2 cut(s) 4, 194
HpaII CCGG 1 cut(s) 50
Hpy188I TCNGA 4 cut(s) 64, 107, 289, 312
HpyAV CCTTC 2 cut(s) 179, 244
HpyCH4V TGCA 2 cut(s) 365, 431
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 251, 437
HpyF3I CTNAG 3 cut(s) 23, 137, 386
Hsp92I GRCGYC 1 cut(s) 148
Hsp92II CATG 2 cut(s) 204, 326
HspAI GCGC 1 cut(s) 148
KasI GGCGCC 1 cut(s) 147
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 2 cut(s) 63, 165
Lsp1109I GCAGC 2 cut(s) 217, 290
LweI GCATC 1 cut(s) 352
MboII GAAGA 1 cut(s) 9
MfeI CAATTG 1 cut(s) 441
MluCI AATT 4 cut(s) 290, 344, 405, 441
Mly113I GGCGCC 1 cut(s) 148
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 8 cut(s) 18, 68, 101, 138, 204, 328, 415, 479
MroNI GCCGGC 1 cut(s) 49
MseI TTAA 2 cut(s) 381, 493
MspI CCGG 1 cut(s) 50
MunI CAATTG 1 cut(s) 441
MwoI GCNNNNNNNGC 3 cut(s) 75, 251, 437
NaeI GCCGGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 148
NgoMIV GCCGGC 1 cut(s) 49
NlaIII CATG 2 cut(s) 204, 326
NlaIV GGNNCC 3 cut(s) 82, 149, 462
NspI RCATGY 1 cut(s) 326
NspV TTCGAA 1 cut(s) 415
PceI AGGCCT 1 cut(s) 214
PdiI GCCGGC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 2 cut(s) 232, 280
PleI GAGTC 1 cut(s) 202
PluTI GGCGCC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 202
PspN4I GGNNCC 3 cut(s) 82, 149, 462
SaqAI TTAA 2 cut(s) 381, 493
SatI GCNGC 2 cut(s) 231, 279
SchI GAGTC 1 cut(s) 203
SetI ASST 6 cut(s) 29, 143, 172, 242, 262, 387
SfaNI GCATC 1 cut(s) 352
SfcI CTRYAG 1 cut(s) 216
SfoI GGCGCC 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 415
Sse9I AATT 4 cut(s) 290, 344, 405, 441
SseBI AGGCCT 1 cut(s) 214
SsiI CCGC 2 cut(s) 78, 469
SspDI GGCGCC 1 cut(s) 147
SspI AATATT 1 cut(s) 479
StuI AGGCCT 1 cut(s) 214
StyI CCWWGG 1 cut(s) 240
TaqI TCGA 1 cut(s) 415
TasI AATT 4 cut(s) 290, 344, 405, 441
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 381, 493
Tru9I TTAA 2 cut(s) 381, 493
TscAI CASTG 1 cut(s) 27
TseI GCWGC 2 cut(s) 230, 278
TspRI CASTG 1 cut(s) 27
XagI CCTNNNNNAGG 1 cut(s) 244
XapI RAATTY 1 cut(s) 405
XceI RCATGY 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.