Rh5AG469900

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0020710
Physical Location & Seq
Reverse (-)
80557329 .. 80558672
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG469900.1

Sequence Viewer

Length: 1344 bp
ATGGAGAGTAGCATGATTTTCCGAGGGTTGAAGCACGTTTGGTTGAATTCAATACAAACAAGGCGCAATCTTGTATTTGATGTTCCAAACATTTACGGAAGTTGGAGACGACCAAGAAAAGATGATGAAGACTTTGTAAGACGTGTGGATCAATTTTTGGAGCGTTACCAAGGTGACAAAGTAGACTCTTTCACATTAGACTATAACTTTAATAAGGAACCATGGCTGCATGAAGTTGTCAGTAAATGGATTGGGTTTGCAATTAGAAAGGGTGTTCAAGAGCTTCGTCTCCCTATGCCGACGAATTTTGGTCCTTTGTATAATGATGATTTCCCTCACCAGTTACTGTCAGAACAACAAGTGAAGGCTGTTGCTCCTCTAAAGCTAAAATCTTTGTCCTTGAAAGGATTTCGTCTAAGAGTTCCTCCTGAATTTGACGGATTCAATCAGCTCACTACTCTTTGTCTGCATTCGGTTAAGGTTGATGGAATTTTTATGTCACATCTCTTCTCTCTTTGTTCTTTCCTTGAACGCTTGACCTTAAAGAATTGCACATGGGAGTACTTCTATTCACATCTGAATATTGTTTCTGGTCGTCTGAATGATTTCAAACTGATCTCTTGTACTGAGCTAGAAAAGATCGAGATTTCTGCACCAAATCTTGCCTCCTTGGAATACATTGCTAAAATCGTGTATCCTCCATCAAGTATTGTTTTCGTCAACACTCCAAAGTTAGCAAAATTGTTGTATCTCACAGACCTAAGCACAATGTTGCCTCAAACACTAAGACAATTAATTCCATCGTGCCCTGCACTTGAGACGCTTGATCTACAGATGTGTGCCGAAAGACCCCAAATCGGCAGCTTACCAACATTTGGAAATCTCAAGCAATTGAACTTGCAAATCGAAAGAGGTTATTACAATAGCATCCCTATTCATCTGAGATGTGTTGTGGACCTCCTCAGGGTTGCACCCCTTTTGGAAGAGCTCACAATAACGATAGTGGGACAAGGGAAGTTTGATGAGACTGATCAACAAGAGATAAGGAGCCTTGATTGTGGATTCACACACAACCAATTGAAGAAAGTTAAGATGCATGGGATCCAAGGTAATTGGTATGAGATAGAGTTTGCTATTTGGATTCTAAAGAATACTACAAACCTTGAGATAATGGTTATTGATCCCAATTGGAAACTATACCTTGGTGATGGTGATTATATTGAGTTTAATTATTCAACCTGTCAAAAAGGAAGACACAGAGCTGTTGTCCGAGAAAAACTAAAGGAAGTAAAAACTGATGCTCGAATTAGAATTCTCAACCGGTGGTCCTTACCAGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

447

Amino Acids

52.05

Weight (kDa)

8.57

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 53 - 398 8.3e-36 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 81 - 253 8.5e-12 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020710)

Species Orthologous Gene IDs
rosa_laevigata RLG00000036237
rosa_multiflora Rmu_sc0005745.1_g000033 Rmu_sc0005745.1_g000034
rosa_samantha Rh5AG469900 Rh5BG489200 Rh5DG499800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 875
AccI GTMKAC 1 cut(s) 183
AclWI GGATC 4 cut(s) 156, 1096, 1109, 1175
AcsI RAATTY 5 cut(s) 46, 304, 431, 489, 1311
AfaI GTAC 2 cut(s) 563, 625
AfiI CCNNNNNNNGG 3 cut(s) 857, 875, 964
AflIII ACRYGT 1 cut(s) 142
AgeI ACCGGT 1 cut(s) 1320
AjiI CACGTC 1 cut(s) 143
AluBI AGCT 7 cut(s) 283, 385, 451, 631, 864, 988, 1262
AluI AGCT 7 cut(s) 283, 385, 451, 631, 864, 988, 1262
Alw21I GWGCWC 1 cut(s) 990
Alw26I GTCTC 4 cut(s) 100, 293, 812, 1019
AlwI GGATC 4 cut(s) 156, 1096, 1109, 1175
AlwNI CAGNNNCTG 1 cut(s) 346
ApeKI GCWGC 2 cut(s) 226, 861
ApoI RAATTY 5 cut(s) 46, 304, 431, 489, 1311
AseI ATTAAT 1 cut(s) 794
AsiGI ACCGGT 1 cut(s) 1320
Asp700I GAANNNNTTC 1 cut(s) 605
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 3 cut(s) 311, 955, 1326
AsuHPI GGTGA 4 cut(s) 185, 329, 1217, 1223
AvaII GGWCC 3 cut(s) 311, 955, 1326
AxyI CCTNAGG 1 cut(s) 962
BaeGI GKGCMC 1 cut(s) 809
BamHI GGATCC 1 cut(s) 1101
BanII GRGCYC 1 cut(s) 990
BbsI GAAGAC 2 cut(s) 135, 1258
Bbv12I GWGCWC 1 cut(s) 990
BbvI GCAGC 2 cut(s) 213, 873
BccI CCATC 4 cut(s) 479, 709, 808, 1202
BcgI CGANNNNNNTGC 2 cut(s) 632, 666
BciVI GTATCC 1 cut(s) 705
BclI TGATCA 1 cut(s) 1030
BcoDI GTCTC 4 cut(s) 100, 293, 812, 1019
BfaI CTAG 1 cut(s) 632
BfmI CTRYAG 1 cut(s) 830
BfuI GTATCC 1 cut(s) 705
BisI GCNGC 2 cut(s) 227, 862
BlsI GCNGC 2 cut(s) 228, 863
BmcAI AGTACT 1 cut(s) 563
Bme18I GGWCC 3 cut(s) 311, 955, 1326
BmgBI CACGTC 1 cut(s) 143
BmgT120I GGNCC 3 cut(s) 311, 955, 1326
BmiI GGNNCC 3 cut(s) 219, 1049, 1103
BmsI GCATC 3 cut(s) 936, 1083, 1288
BpiI GAAGAC 2 cut(s) 135, 1258
Bpu10I CCTNAGC 1 cut(s) 761
BpuEI CTTGAG 3 cut(s) 836, 869, 1184
BsaJI CCNNGG 6 cut(s) 22, 169, 221, 669, 1105, 1201
BsaWI WCCGGW 1 cut(s) 1320
Bsc4I CCNNNNNNNGG 3 cut(s) 857, 875, 964
Bse118I RCCGGY 1 cut(s) 1320
Bse1I ACTGG 1 cut(s) 340
Bse21I CCTNAGG 1 cut(s) 962
Bse3DI GCAATG 1 cut(s) 678
BseDI CCNNGG 6 cut(s) 22, 169, 221, 669, 1105, 1201
BseGI GGATG 1 cut(s) 927
BseLI CCNNNNNNNGG 3 cut(s) 857, 875, 964
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 3 cut(s) 618, 932, 976
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 2 cut(s) 366, 950
BseSI GKGCMC 1 cut(s) 809
BseXI GCAGC 2 cut(s) 213, 873
BsgI GTGCAG 2 cut(s) 636, 795
BshTI ACCGGT 1 cut(s) 1320
BsiHKAI GWGCWC 1 cut(s) 990
BsiSI CCGG 1 cut(s) 1321
BslFI GGGAC 1 cut(s) 1020
BslI CCNNNNNNNGG 3 cut(s) 857, 875, 964
BsmAI GTCTC 4 cut(s) 100, 293, 812, 1019
BsmBI CGTCTC 3 cut(s) 100, 293, 812
BsmFI GGGAC 1 cut(s) 1020
BsmI GAATGC 1 cut(s) 469
Bsp1286I GDGCHC 2 cut(s) 809, 990
Bsp143I GATC 7 cut(s) 148, 615, 639, 826, 1030, 1101, 1180
Bsp19I CCATGG 1 cut(s) 221
BspCNI CTCAG 3 cut(s) 619, 933, 975
BspLI GGNNCC 3 cut(s) 219, 1049, 1103
BspPI GGATC 4 cut(s) 156, 1096, 1109, 1175
BspQI GCTCTTC 1 cut(s) 978
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 1320
BsrI ACTGG 1 cut(s) 340
BssAI RCCGGY 1 cut(s) 1320
BssECI CCNNGG 6 cut(s) 22, 169, 221, 669, 1105, 1201
BssMI GATC 7 cut(s) 148, 615, 639, 826, 1030, 1101, 1180
BssT1I CCWWGG 5 cut(s) 169, 221, 669, 1105, 1201
Bst4CI ACNGT 1 cut(s) 348
Bst6I CTCTTC 2 cut(s) 512, 978
BstDEI CTNAG 6 cut(s) 416, 627, 761, 785, 941, 962
BstDSI CCRYGG 1 cut(s) 221
BstF5I GGATG 1 cut(s) 927
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 7 cut(s) 151, 618, 642, 829, 1033, 1104, 1183
BstMAI GTCTC 4 cut(s) 100, 293, 812, 1019
BstMBI GATC 7 cut(s) 148, 615, 639, 826, 1030, 1101, 1180
BstSFI CTRYAG 1 cut(s) 830
BstSLI GKGCMC 1 cut(s) 809
BstV1I GCAGC 2 cut(s) 213, 873
BstV2I GAAGAC 2 cut(s) 135, 1258
BstX2I RGATCY 1 cut(s) 1101
BstYI RGATCY 1 cut(s) 1101
Bsu36I CCTNAGG 1 cut(s) 962
BsuI GTATCC 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 221
BtrI CACGTC 1 cut(s) 143
BtsCI GGATG 1 cut(s) 927
CaiI CAGNNNCTG 1 cut(s) 346
CfoI GCGC 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 1320
Cfr13I GGNCC 3 cut(s) 311, 955, 1326
CseI GACGC 1 cut(s) 829
Csp6I GTAC 2 cut(s) 562, 624
CspAI ACCGGT 1 cut(s) 1320
CviAII CATG 5 cut(s) 13, 222, 230, 555, 1097
CviQI GTAC 2 cut(s) 562, 624
DdeI CTNAG 6 cut(s) 416, 627, 761, 785, 941, 962
DpnI GATC 7 cut(s) 150, 617, 641, 828, 1032, 1103, 1182
DpnII GATC 7 cut(s) 148, 615, 639, 826, 1030, 1101, 1180
Eam1104I CTCTTC 2 cut(s) 512, 978
EarI CTCTTC 2 cut(s) 512, 978
Ecl136II GAGCTC 1 cut(s) 988
Eco130I CCWWGG 5 cut(s) 169, 221, 669, 1105, 1201
Eco24I GRGCYC 1 cut(s) 990
Eco47I GGWCC 3 cut(s) 311, 955, 1326
Eco53kI GAGCTC 1 cut(s) 988
Eco81I CCTNAGG 1 cut(s) 962
EcoICRI GAGCTC 1 cut(s) 988
EcoRI GAATTC 2 cut(s) 46, 1311
EcoT14I CCWWGG 5 cut(s) 169, 221, 669, 1105, 1201
EcoT22I ATGCAT 1 cut(s) 1098
EcoT38I GRGCYC 1 cut(s) 990
ErhI CCWWGG 5 cut(s) 169, 221, 669, 1105, 1201
Esp3I CGTCTC 3 cut(s) 100, 293, 812
FaeI CATG 5 cut(s) 16, 225, 233, 558, 1100
FaqI GGGAC 1 cut(s) 1020
FatI CATG 5 cut(s) 12, 221, 229, 554, 1096
FbaI TGATCA 1 cut(s) 1030
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 2 cut(s) 227, 862
FokI GGATG 1 cut(s) 914
FriOI GRGCYC 1 cut(s) 990
Fsp4HI GCNGC 2 cut(s) 227, 862
FspBI CTAG 1 cut(s) 632
GlaI GCGC 1 cut(s) 65
GluI GCNGC 2 cut(s) 227, 862
HapII CCGG 1 cut(s) 1321
HgaI GACGC 1 cut(s) 829
HhaI GCGC 1 cut(s) 66
Hin1II CATG 5 cut(s) 16, 225, 233, 558, 1100
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HinfI GANTC 4 cut(s) 185, 441, 1062, 1141
HpaII CCGG 1 cut(s) 1321
HphI GGTGA 4 cut(s) 185, 329, 1217, 1223
Hpy166II GTNNAC 3 cut(s) 184, 721, 955
Hpy188I TCNGA 6 cut(s) 23, 352, 579, 600, 942, 1271
Hpy188III TCNNGA 3 cut(s) 278, 428, 643
Hpy8I GTNNAC 3 cut(s) 184, 721, 955
Hpy99I CGWCG 1 cut(s) 304
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 1 cut(s) 348
HpyCH4IV ACGT 2 cut(s) 36, 142
HpyCH4V TGCA 9 cut(s) 229, 260, 469, 552, 653, 812, 901, 971, 1096
HpyF3I CTNAG 6 cut(s) 416, 627, 761, 785, 941, 962
HpySE526I ACGT 2 cut(s) 36, 142
Hsp92II CATG 5 cut(s) 16, 225, 233, 558, 1100
HspAI GCGC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 1030
Kzo9I GATC 7 cut(s) 148, 615, 639, 826, 1030, 1101, 1180
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 3 cut(s) 160, 379, 1047
LpnPI CCDG 7 cut(s) 353, 441, 576, 822, 949, 1252, 1334
Lsp1109I GCAGC 2 cut(s) 213, 873
LweI GCATC 3 cut(s) 936, 1083, 1288
MaeI CTAG 1 cut(s) 632
MaeII ACGT 2 cut(s) 36, 142
MaeIII GTNAC 4 cut(s) 164, 173, 342, 498
MalI GATC 7 cut(s) 150, 617, 641, 828, 1032, 1103, 1182
MboI GATC 7 cut(s) 148, 615, 639, 826, 1030, 1101, 1180
MboII GAAGA 5 cut(s) 140, 499, 995, 1093, 1263
MfeI CAATTG 3 cut(s) 890, 1076, 1186
MflI RGATCY 1 cut(s) 1101
MhlI GDGCHC 2 cut(s) 809, 990
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 83
Mph1103I ATGCAT 1 cut(s) 1098
MroXI GAANNNNTTC 1 cut(s) 605
MseI TTAA 6 cut(s) 210, 477, 542, 794, 1089, 1227
MspI CCGG 1 cut(s) 1321
MunI CAATTG 3 cut(s) 890, 1076, 1186
Mva1269I GAATGC 1 cut(s) 469
NcoI CCATGG 1 cut(s) 221
NdeII GATC 7 cut(s) 148, 615, 639, 826, 1030, 1101, 1180
NlaIII CATG 5 cut(s) 16, 225, 233, 558, 1100
NlaIV GGNNCC 3 cut(s) 219, 1049, 1103
NmuCI GTSAC 2 cut(s) 173, 498
NsiI ATGCAT 1 cut(s) 1098
PciSI GCTCTTC 1 cut(s) 978
PctI GAATGC 1 cut(s) 469
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 3 cut(s) 441, 1062, 1141
PflMI CCANNNNNTGG 1 cut(s) 875
PinAI ACCGGT 1 cut(s) 1320
PkrI GCNGC 2 cut(s) 228, 863
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PshBI ATTAAT 1 cut(s) 794
Psp124BI GAGCTC 1 cut(s) 990
PspN4I GGNNCC 3 cut(s) 219, 1049, 1103
PspPI GGNCC 3 cut(s) 311, 955, 1326
PsrI GAACNNNNNNTAC 2 cut(s) 66, 98
PstNI CAGNNNCTG 1 cut(s) 346
PsuI RGATCY 1 cut(s) 1101
RsaI GTAC 2 cut(s) 563, 625
RsaNI GTAC 2 cut(s) 562, 624
SacI GAGCTC 1 cut(s) 990
SapI GCTCTTC 1 cut(s) 978
SaqAI TTAA 6 cut(s) 210, 477, 542, 794, 1089, 1227
SatI GCNGC 2 cut(s) 227, 862
Sau3AI GATC 7 cut(s) 148, 615, 639, 826, 1030, 1101, 1180
Sau96I GGNCC 3 cut(s) 311, 955, 1326
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 2 cut(s) 809, 990
SfaNI GCATC 3 cut(s) 936, 1083, 1288
SfcI CTRYAG 1 cut(s) 830
SinI GGWCC 3 cut(s) 311, 955, 1326
SmlI CTYRAG 3 cut(s) 815, 884, 1163
SmoI CTYRAG 3 cut(s) 815, 884, 1163
SspI AATATT 1 cut(s) 583
SspMI CTAG 1 cut(s) 632
SstI GAGCTC 1 cut(s) 990
StyI CCWWGG 5 cut(s) 169, 221, 669, 1105, 1201
TaaI ACNGT 1 cut(s) 348
TaiI ACGT 2 cut(s) 39, 145
TaqI TCGA 3 cut(s) 642, 906, 1303
TatI WGTACW 2 cut(s) 561, 623
TfiI GAWTC 3 cut(s) 441, 1062, 1141
Tru1I TTAA 6 cut(s) 210, 477, 542, 794, 1089, 1227
Tru9I TTAA 6 cut(s) 210, 477, 542, 794, 1089, 1227
TseFI GTSAC 2 cut(s) 173, 498
TseI GCWGC 2 cut(s) 226, 861
Tsp45I GTSAC 2 cut(s) 173, 498
TspDTI ATGAA 3 cut(s) 141, 246, 926
TspGWI ACGGA 2 cut(s) 111, 453
Van91I CCANNNNNTGG 1 cut(s) 875
VpaK11BI GGWCC 3 cut(s) 311, 955, 1326
VspI ATTAAT 1 cut(s) 794
XapI RAATTY 5 cut(s) 46, 304, 431, 489, 1311
XmiI GTMKAC 1 cut(s) 183
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 1 cut(s) 632
ZrmI AGTACT 1 cut(s) 563
Zsp2I ATGCAT 1 cut(s) 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.