Rh5BG489200

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0020710
Physical Location & Seq
Reverse (-)
77900517 .. 77911624
11108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG489200.1

Sequence Viewer

Length: 690 bp
ATGGACCAGGCTATTCTAGATTGTTGTCATCTGGGCGCAACGGATCAAAGCCTAAAATTAGCTAGTGTATCTTGTAACCAGGAGAGAAGTAAGTTTGAGGAGTTGCCGGATTGCATTCTGGAATCCATAGTTTCATTGCTCCCCCTAAGAGATGCAATGGAGAGTAGCATGATTTTCCGAGGGTTGAAGCACGTTTGGTTGAATTCAATACAAACAAGGCGCAATCTTGTATTTGATGTTCCAAACATTTACGGAAGTTGGAGACGACCAAGAAAAGATGATGAAGAATTTGTAAGACGTGTGGATCCATTTTTGGAGCGTTACCAAGGTGACAAAGTAGACTTTTTCACATTAGACTGTAACTTTAATAAGGAACCATGGCTGCATGAAGTTGTCAGTAAATGGATTGGTTTTGCAATTAGAAAGGGTGTTCAAGAGCTTCGTCTCCCTATGCCGACGAATTTTGGTCCATTGTATAATGATGATTTCCCTCACCAGTTACTGTCAGAACAACAAGTGAAGGCTGTTGCTCCTCTAAAGCTAAAATCTTTGTCCTTGAAAGGATTTCGTCTAAGAGTTCCTCCTGAATTTGACGGATTCAATCAGCTCACTACTCTTTGTCTGCATTCGGTTAAGGTTGATGGAATTTTTAATTATGTCACATCTCTTCTCTCTTTGTTCTTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

229

Amino Acids

26.53

Weight (kDa)

6.52

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 105 - 215 6.6e-08 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020710)

Species Orthologous Gene IDs
rosa_laevigata RLG00000036237
rosa_multiflora Rmu_sc0005745.1_g000033 Rmu_sc0005745.1_g000034
rosa_samantha Rh5AG469900 Rh5BG489200 Rh5DG499800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 339
AclWI GGATC 3 cut(s) 51, 299, 312
AcsI RAATTY 5 cut(s) 202, 287, 460, 587, 645
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 6 cut(s) 187, 202, 207, 434, 559, 601
AjiI CACGTC 1 cut(s) 299
AjnI CCWGG 2 cut(s) 6, 78
AluBI AGCT 4 cut(s) 62, 439, 541, 607
AluI AGCT 4 cut(s) 62, 439, 541, 607
Alw26I GTCTC 2 cut(s) 256, 449
AlwI GGATC 3 cut(s) 51, 299, 312
AlwNI CAGNNNCTG 1 cut(s) 502
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 5 cut(s) 202, 287, 460, 587, 645
AspLEI GCGC 2 cut(s) 38, 222
AspS9I GGNCC 2 cut(s) 4, 467
AsuHPI GGTGA 2 cut(s) 341, 485
AvaII GGWCC 2 cut(s) 4, 467
BamHI GGATCC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 369
BccI CCATC 1 cut(s) 635
BciT130I CCWGG 2 cut(s) 8, 80
BcoDI GTCTC 2 cut(s) 256, 449
BfaI CTAG 2 cut(s) 17, 63
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
Bme1390I CCNGG 2 cut(s) 8, 80
Bme18I GGWCC 2 cut(s) 4, 467
BmgBI CACGTC 1 cut(s) 299
BmgT120I GGNCC 2 cut(s) 4, 467
BmiI GGNNCC 2 cut(s) 306, 375
BmrFI CCNGG 2 cut(s) 8, 80
BmsI GCATC 1 cut(s) 142
BsaJI CCNNGG 3 cut(s) 178, 325, 377
Bse1I ACTGG 1 cut(s) 496
Bse3DI GCAATG 2 cut(s) 134, 162
BseBI CCWGG 2 cut(s) 8, 80
BseDI CCNNGG 3 cut(s) 178, 325, 377
BseMI GCAATG 2 cut(s) 134, 162
BseNI ACTGG 1 cut(s) 496
BseRI GAGGAG 2 cut(s) 113, 522
BseXI GCAGC 1 cut(s) 369
BsiSI CCGG 1 cut(s) 107
BsmAI GTCTC 2 cut(s) 256, 449
BsmBI CGTCTC 2 cut(s) 256, 449
BsmI GAATGC 2 cut(s) 114, 625
Bsp143I GATC 2 cut(s) 43, 304
Bsp19I CCATGG 1 cut(s) 377
BspLI GGNNCC 2 cut(s) 306, 375
BspPI GGATC 3 cut(s) 51, 299, 312
BsrDI GCAATG 2 cut(s) 134, 162
BsrI ACTGG 1 cut(s) 496
BssECI CCNNGG 3 cut(s) 178, 325, 377
BssMI GATC 2 cut(s) 43, 304
BssT1I CCWWGG 2 cut(s) 325, 377
Bst2UI CCWGG 2 cut(s) 8, 80
Bst4CI ACNGT 2 cut(s) 359, 504
Bst6I CTCTTC 1 cut(s) 672
BstDEI CTNAG 2 cut(s) 146, 572
BstDSI CCRYGG 1 cut(s) 377
BstHHI GCGC 2 cut(s) 38, 222
BstKTI GATC 2 cut(s) 46, 307
BstMAI GTCTC 2 cut(s) 256, 449
BstMBI GATC 2 cut(s) 43, 304
BstNI CCWGG 2 cut(s) 8, 80
BstSCI CCNGG 2 cut(s) 6, 78
BstV1I GCAGC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BtgI CCRYGG 1 cut(s) 377
BtrI CACGTC 1 cut(s) 299
CaiI CAGNNNCTG 1 cut(s) 502
CfoI GCGC 2 cut(s) 38, 222
Cfr13I GGNCC 2 cut(s) 4, 467
CviAII CATG 3 cut(s) 169, 378, 386
CviJI RGCY 8 cut(s) 11, 51, 62, 382, 439, 524, 541, 607
CviKI_1 RGCY 8 cut(s) 11, 51, 62, 382, 439, 524, 541, 607
DdeI CTNAG 2 cut(s) 146, 572
DpnI GATC 2 cut(s) 45, 306
DpnII GATC 2 cut(s) 43, 304
Eam1104I CTCTTC 1 cut(s) 672
EarI CTCTTC 1 cut(s) 672
Eco130I CCWWGG 2 cut(s) 325, 377
Eco47I GGWCC 2 cut(s) 4, 467
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 6, 78
EcoT14I CCWWGG 2 cut(s) 325, 377
ErhI CCWWGG 2 cut(s) 325, 377
Esp3I CGTCTC 2 cut(s) 256, 449
FaeI CATG 3 cut(s) 172, 381, 389
FaiI YATR 7 cut(s) 128, 170, 379, 387, 452, 477, 657
FatI CATG 3 cut(s) 168, 377, 385
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 1 cut(s) 383
Fsp4HI GCNGC 1 cut(s) 383
FspBI CTAG 2 cut(s) 17, 63
GlaI GCGC 2 cut(s) 37, 221
GluI GCNGC 1 cut(s) 383
HapII CCGG 1 cut(s) 107
HhaI GCGC 2 cut(s) 38, 222
Hin1II CATG 3 cut(s) 172, 381, 389
Hin6I GCGC 2 cut(s) 36, 220
HinP1I GCGC 2 cut(s) 36, 220
HinfI GANTC 2 cut(s) 122, 597
HpaII CCGG 1 cut(s) 107
HphI GGTGA 2 cut(s) 341, 485
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 179, 508
Hpy188III TCNNGA 4 cut(s) 17, 119, 434, 584
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 1 cut(s) 460
HpyAV CCTTC 1 cut(s) 514
HpyCH4III ACNGT 2 cut(s) 359, 504
HpyCH4IV ACGT 2 cut(s) 192, 298
HpyCH4V TGCA 5 cut(s) 114, 155, 385, 416, 625
HpyF3I CTNAG 2 cut(s) 146, 572
HpySE526I ACGT 2 cut(s) 192, 298
Hsp92II CATG 3 cut(s) 172, 381, 389
HspAI GCGC 2 cut(s) 36, 220
Kzo9I GATC 2 cut(s) 43, 304
LmnI GCTCC 3 cut(s) 144, 316, 535
LpnPI CCDG 8 cut(s) 17, 20, 65, 92, 104, 120, 509, 597
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 1 cut(s) 142
MaeI CTAG 2 cut(s) 17, 63
MaeII ACGT 2 cut(s) 192, 298
MaeIII GTNAC 6 cut(s) 74, 320, 329, 359, 498, 658
MalI GATC 2 cut(s) 45, 306
MboI GATC 2 cut(s) 43, 304
MboII GAAGA 2 cut(s) 296, 659
MflI RGATCY 1 cut(s) 304
MluCI AATT 8 cut(s) 56, 202, 287, 417, 460, 587, 645, 652
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 5 cut(s) 91, 173, 501, 543, 591
MseI TTAA 3 cut(s) 366, 633, 651
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 2 cut(s) 8, 80
Mva1269I GAATGC 2 cut(s) 114, 625
MvaI CCWGG 2 cut(s) 8, 80
NcoI CCATGG 1 cut(s) 377
NdeII GATC 2 cut(s) 43, 304
NlaIII CATG 3 cut(s) 172, 381, 389
NlaIV GGNNCC 2 cut(s) 306, 375
NmuCI GTSAC 2 cut(s) 329, 658
PctI GAATGC 2 cut(s) 114, 625
PfeI GAWTC 2 cut(s) 122, 597
PkrI GCNGC 1 cut(s) 384
Psp6I CCWGG 2 cut(s) 6, 78
PspGI CCWGG 2 cut(s) 6, 78
PspN4I GGNNCC 2 cut(s) 306, 375
PspPI GGNCC 2 cut(s) 4, 467
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
PstNI CAGNNNCTG 1 cut(s) 502
PsuI RGATCY 1 cut(s) 304
SaqAI TTAA 3 cut(s) 366, 633, 651
SatI GCNGC 1 cut(s) 383
Sau3AI GATC 2 cut(s) 43, 304
Sau96I GGNCC 2 cut(s) 4, 467
ScrFI CCNGG 2 cut(s) 8, 80
SetI ASST 8 cut(s) 64, 195, 301, 331, 441, 543, 609, 639
SfaNI GCATC 1 cut(s) 142
SinI GGWCC 2 cut(s) 4, 467
Sse9I AATT 8 cut(s) 56, 202, 287, 417, 460, 587, 645, 652
SspMI CTAG 2 cut(s) 17, 63
StyD4I CCNGG 2 cut(s) 6, 78
StyI CCWWGG 2 cut(s) 325, 377
TaaI ACNGT 2 cut(s) 359, 504
TaiI ACGT 2 cut(s) 195, 301
TasI AATT 8 cut(s) 56, 202, 287, 417, 460, 587, 645, 652
TfiI GAWTC 2 cut(s) 122, 597
Tru1I TTAA 3 cut(s) 366, 633, 651
Tru9I TTAA 3 cut(s) 366, 633, 651
TseFI GTSAC 2 cut(s) 329, 658
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 2 cut(s) 329, 658
TspDTI ATGAA 3 cut(s) 123, 297, 402
TspGWI ACGGA 3 cut(s) 56, 267, 609
VpaK11BI GGWCC 2 cut(s) 4, 467
XapI RAATTY 5 cut(s) 202, 287, 460, 587, 645
XbaI TCTAGA 1 cut(s) 16
XmiI GTMKAC 1 cut(s) 339
XspI CTAG 2 cut(s) 17, 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.