Rh5DG499800

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0020710
Physical Location & Seq
Reverse (-)
78377878 .. 78379338
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG499800.1

Sequence Viewer

Length: 1461 bp
ATGGATCAAAGCCTAAAATTAGCTAGTGTATCTTGTAACCAGGAGAGAAGTAAGTTTGAGGAGTTGCCGGATTGCATTCTGGAATCCATAGTTTCATTTCTCCCGCTAAGAGATGCAATGGAGAGTAGCATGATTTTCCGAGGGTTGAAGCACATTTCGTTGAATTCAATACAAACAAGGCGCAATCTTGTATTTGATGTTCCAAACATTTACGGAAGTTGGAGACAACCAAGAAAAGATGATGAAGACTTTGTAAGACGTGTGGATCAATTTTTGGAGCGTTACCAAGGTGACAAAGTAGACTCTTTCACATTAGACTATAACTTTAATAAGGAACCATGGCTGCATGAAGTTGTCAGTAAATGGATTGGTTTTGCAATTAGAAAGGGTGTTCAAGAGCTTCGTCTCCCTATGCCGACGAATTTTGGTCCTTTGTATAATGATGATTTCCCTCACCAGTTACTGTCAGAACAACAAGTGAAGGCTGTTGCTCCTCTAAAGCTAAAATCTTTGTCCTTGAAAGGATTTCGTCTAAGAGTTCCTCCTGAATTTGACGGATTCAATCAGCTCACTACTCTTTGTCTGCATTCGGTTAAGGTTGATGGAATTTTTATGTCACATCTCTTCTCTCTTGGTTCTTTCCTTGAACGCTTGACCTTAAAGAATTGCACATGGGAGTACTTCTATTCACATCTGAATATTGTTTCTGGTCGTCTGAATGATTTCAAACTGATCTCTTGTACTGAGCTAGAAAAGATCGAGATTTCTGCACCAAATCTTGCCTCCTTAGAATACATTGCTAAATCCGTGTATCCTCCATCAAGTATTGTTTTCGTCAACACTCCAAAGTTAGCAAAATTATTGTATCTCACAGACCTAAGCACAATGTTGCCTCAAACACTAAGACAATTAATTCCATCGTGCCCTGCACTTGAGACGCTTGATCTACAGATGTGTGCCGAAAGACCCCAAATCGGCAGCTTACCAACATTTGGAAATCTCAAGCAATTGAACTTGCAAATCAAAAGAGGTTATTACAATAGCATCCCTATTCATCTGAGATGTGTTGTGGACCTCCTCAAGGTTGCACCCCTTTTGGAAGAGCTCACAATAACGATAGAGGGACAAAGGGAGTTTGATGAGACTGATCATCAAGAGATAAGGAACCTTGATTGTGGATTCACACACAACCAATTGAAGAAAGTTAAGATGCATGGGATCCAAGGTAATTGGTATGAGATAGAGTTTGCTATTTGGATTCTAAAGAATACTACAAACCTTGAGATAATGGTTATTGATCCCAATTGGAAACGCTACCTTGGTGATGGTGATTTTATTGAGCTTAATCATTCAACCTGGCAAAAAGGAAGACACAGAGCTGTTGTCCGAGAAAAACTAAAGGAAGTAAAAACTGATGCTCGAATTAGAATTCTCAACCGGTGGTCCTTACCAGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

486

Amino Acids

56.45

Weight (kDa)

7.98

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 92 - 437 1.7e-35 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 120 - 292 8.2e-10 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020710)

Species Orthologous Gene IDs
rosa_laevigata RLG00000036237
rosa_multiflora Rmu_sc0005745.1_g000033 Rmu_sc0005745.1_g000034
rosa_samantha Rh5AG469900 Rh5BG489200 Rh5DG499800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 992
AccI GTMKAC 1 cut(s) 300
AciI CCGC 1 cut(s) 104
AclWI GGATC 5 cut(s) 12, 273, 1213, 1226, 1292
AcsI RAATTY 5 cut(s) 163, 421, 548, 606, 1428
AfaI GTAC 2 cut(s) 680, 742
AfiI CCNNNNNNNGG 3 cut(s) 974, 992, 1081
AflIII ACRYGT 1 cut(s) 259
AgeI ACCGGT 1 cut(s) 1437
AjiI CACGTC 1 cut(s) 260
AjnI CCWGG 2 cut(s) 39, 1355
AluBI AGCT 9 cut(s) 23, 400, 502, 568, 748, 981, 1105, 1342, 1379
AluI AGCT 9 cut(s) 23, 400, 502, 568, 748, 981, 1105, 1342, 1379
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 4 cut(s) 217, 410, 929, 1136
AlwI GGATC 5 cut(s) 12, 273, 1213, 1226, 1292
AlwNI CAGNNNCTG 1 cut(s) 463
ApeKI GCWGC 2 cut(s) 343, 978
ApoI RAATTY 5 cut(s) 163, 421, 548, 606, 1428
AseI ATTAAT 1 cut(s) 911
AsiGI ACCGGT 1 cut(s) 1437
Asp700I GAANNNNTTC 1 cut(s) 722
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 3 cut(s) 428, 1072, 1443
AsuHPI GGTGA 4 cut(s) 302, 446, 1334, 1340
AvaII GGWCC 3 cut(s) 428, 1072, 1443
BaeGI GKGCMC 1 cut(s) 926
BamHI GGATCC 1 cut(s) 1218
BanII GRGCYC 1 cut(s) 1107
BbsI GAAGAC 2 cut(s) 252, 1375
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 2 cut(s) 330, 990
BccI CCATC 4 cut(s) 596, 826, 925, 1319
BcgI CGANNNNNNTGC 2 cut(s) 749, 783
BciT130I CCWGG 2 cut(s) 41, 1357
BciVI GTATCC 1 cut(s) 822
BclI TGATCA 1 cut(s) 1147
BcoDI GTCTC 4 cut(s) 217, 410, 929, 1136
BfaI CTAG 2 cut(s) 24, 749
BfmI CTRYAG 1 cut(s) 947
BfuI GTATCC 1 cut(s) 822
BisI GCNGC 2 cut(s) 344, 979
BlsI GCNGC 2 cut(s) 345, 980
BmcAI AGTACT 1 cut(s) 680
Bme1390I CCNGG 2 cut(s) 41, 1357
Bme18I GGWCC 3 cut(s) 428, 1072, 1443
BmgBI CACGTC 1 cut(s) 260
BmgT120I GGNCC 3 cut(s) 428, 1072, 1443
BmiI GGNNCC 3 cut(s) 336, 1166, 1220
BmrFI CCNGG 2 cut(s) 41, 1357
BmsI GCATC 4 cut(s) 103, 1053, 1200, 1405
BpiI GAAGAC 2 cut(s) 252, 1375
Bpu10I CCTNAGC 1 cut(s) 878
BpuEI CTTGAG 4 cut(s) 953, 986, 1064, 1301
BsaJI CCNNGG 5 cut(s) 139, 286, 338, 1222, 1318
BsaWI WCCGGW 1 cut(s) 1437
Bsc4I CCNNNNNNNGG 3 cut(s) 974, 992, 1081
Bse118I RCCGGY 1 cut(s) 1437
Bse1I ACTGG 1 cut(s) 457
Bse3DI GCAATG 2 cut(s) 123, 795
BseBI CCWGG 2 cut(s) 41, 1357
BseDI CCNNGG 5 cut(s) 139, 286, 338, 1222, 1318
BseGI GGATG 1 cut(s) 1044
BseLI CCNNNNNNNGG 3 cut(s) 974, 992, 1081
BseMI GCAATG 2 cut(s) 123, 795
BseMII CTCAG 2 cut(s) 735, 1049
BseNI ACTGG 1 cut(s) 457
BseRI GAGGAG 3 cut(s) 74, 483, 1067
BseSI GKGCMC 1 cut(s) 926
BseXI GCAGC 2 cut(s) 330, 990
BsgI GTGCAG 2 cut(s) 753, 912
BshTI ACCGGT 1 cut(s) 1437
BsiHKAI GWGCWC 1 cut(s) 1107
BsiSI CCGG 2 cut(s) 68, 1438
BslFI GGGAC 1 cut(s) 1137
BslI CCNNNNNNNGG 3 cut(s) 974, 992, 1081
BsmAI GTCTC 4 cut(s) 217, 410, 929, 1136
BsmBI CGTCTC 2 cut(s) 410, 929
BsmFI GGGAC 1 cut(s) 1137
BsmI GAATGC 2 cut(s) 75, 586
Bsp1286I GDGCHC 2 cut(s) 926, 1107
Bsp143I GATC 8 cut(s) 4, 265, 732, 756, 943, 1147, 1218, 1297
Bsp19I CCATGG 1 cut(s) 338
BspACI CCGC 1 cut(s) 104
BspCNI CTCAG 2 cut(s) 736, 1050
BspLI GGNNCC 3 cut(s) 336, 1166, 1220
BspPI GGATC 5 cut(s) 12, 273, 1213, 1226, 1292
BspQI GCTCTTC 1 cut(s) 1095
BsrDI GCAATG 2 cut(s) 123, 795
BsrFI RCCGGY 1 cut(s) 1437
BsrI ACTGG 1 cut(s) 457
BssAI RCCGGY 1 cut(s) 1437
BssECI CCNNGG 5 cut(s) 139, 286, 338, 1222, 1318
BssMI GATC 8 cut(s) 4, 265, 732, 756, 943, 1147, 1218, 1297
BssT1I CCWWGG 4 cut(s) 286, 338, 1222, 1318
Bst2UI CCWGG 2 cut(s) 41, 1357
Bst4CI ACNGT 1 cut(s) 465
Bst6I CTCTTC 2 cut(s) 629, 1095
BstDEI CTNAG 7 cut(s) 107, 533, 744, 787, 878, 902, 1058
BstDSI CCRYGG 1 cut(s) 338
BstENI CCTNNNNNAGG 1 cut(s) 1079
BstF5I GGATG 1 cut(s) 1044
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 8 cut(s) 7, 268, 735, 759, 946, 1150, 1221, 1300
BstMAI GTCTC 4 cut(s) 217, 410, 929, 1136
BstMBI GATC 8 cut(s) 4, 265, 732, 756, 943, 1147, 1218, 1297
BstNI CCWGG 2 cut(s) 41, 1357
BstSCI CCNGG 2 cut(s) 39, 1355
BstSFI CTRYAG 1 cut(s) 947
BstSLI GKGCMC 1 cut(s) 926
BstV1I GCAGC 2 cut(s) 330, 990
BstV2I GAAGAC 2 cut(s) 252, 1375
BstX2I RGATCY 1 cut(s) 1218
BstYI RGATCY 1 cut(s) 1218
BsuI GTATCC 1 cut(s) 822
BtgI CCRYGG 1 cut(s) 338
BtrI CACGTC 1 cut(s) 260
BtsCI GGATG 1 cut(s) 1044
CaiI CAGNNNCTG 1 cut(s) 463
CfoI GCGC 1 cut(s) 183
Cfr10I RCCGGY 1 cut(s) 1437
Cfr13I GGNCC 3 cut(s) 428, 1072, 1443
CseI GACGC 1 cut(s) 946
Csp6I GTAC 2 cut(s) 679, 741
CspAI ACCGGT 1 cut(s) 1437
CviAII CATG 5 cut(s) 130, 339, 347, 672, 1214
CviQI GTAC 2 cut(s) 679, 741
DdeI CTNAG 7 cut(s) 107, 533, 744, 787, 878, 902, 1058
DpnI GATC 8 cut(s) 6, 267, 734, 758, 945, 1149, 1220, 1299
DpnII GATC 8 cut(s) 4, 265, 732, 756, 943, 1147, 1218, 1297
Eam1104I CTCTTC 2 cut(s) 629, 1095
EarI CTCTTC 2 cut(s) 629, 1095
Ecl136II GAGCTC 1 cut(s) 1105
Eco130I CCWWGG 4 cut(s) 286, 338, 1222, 1318
Eco24I GRGCYC 1 cut(s) 1107
Eco47I GGWCC 3 cut(s) 428, 1072, 1443
Eco53kI GAGCTC 1 cut(s) 1105
EcoICRI GAGCTC 1 cut(s) 1105
EcoNI CCTNNNNNAGG 1 cut(s) 1079
EcoRI GAATTC 2 cut(s) 163, 1428
EcoRII CCWGG 2 cut(s) 39, 1355
EcoT14I CCWWGG 4 cut(s) 286, 338, 1222, 1318
EcoT22I ATGCAT 1 cut(s) 1215
EcoT38I GRGCYC 1 cut(s) 1107
ErhI CCWWGG 4 cut(s) 286, 338, 1222, 1318
Esp3I CGTCTC 2 cut(s) 410, 929
FaeI CATG 5 cut(s) 133, 342, 350, 675, 1217
FaqI GGGAC 1 cut(s) 1137
FatI CATG 5 cut(s) 129, 338, 346, 671, 1213
FauI CCCGC 1 cut(s) 111
FbaI TGATCA 1 cut(s) 1147
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 344, 979
FokI GGATG 1 cut(s) 1031
FriOI GRGCYC 1 cut(s) 1107
Fsp4HI GCNGC 2 cut(s) 344, 979
FspBI CTAG 2 cut(s) 24, 749
GlaI GCGC 1 cut(s) 182
GluI GCNGC 2 cut(s) 344, 979
HapII CCGG 2 cut(s) 68, 1438
HgaI GACGC 1 cut(s) 946
HhaI GCGC 1 cut(s) 183
Hin1II CATG 5 cut(s) 133, 342, 350, 675, 1217
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HincII GTYRAC 1 cut(s) 838
HindII GTYRAC 1 cut(s) 838
HinfI GANTC 5 cut(s) 83, 302, 558, 1179, 1258
HpaII CCGG 2 cut(s) 68, 1438
HphI GGTGA 4 cut(s) 302, 446, 1334, 1340
Hpy166II GTNNAC 3 cut(s) 301, 838, 1072
Hpy188I TCNGA 6 cut(s) 140, 469, 696, 717, 1059, 1388
Hpy188III TCNNGA 5 cut(s) 80, 395, 545, 760, 1154
Hpy8I GTNNAC 3 cut(s) 301, 838, 1072
Hpy99I CGWCG 1 cut(s) 421
HpyAV CCTTC 1 cut(s) 475
HpyCH4III ACNGT 1 cut(s) 465
HpyCH4IV ACGT 1 cut(s) 259
HpyF3I CTNAG 7 cut(s) 107, 533, 744, 787, 878, 902, 1058
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 5 cut(s) 133, 342, 350, 675, 1217
HspAI GCGC 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 8 cut(s) 4, 265, 732, 756, 943, 1147, 1218, 1297
LguI GCTCTTC 1 cut(s) 1095
LmnI GCTCC 2 cut(s) 277, 496
Lsp1109I GCAGC 2 cut(s) 330, 990
LweI GCATC 4 cut(s) 103, 1053, 1200, 1405
MaeI CTAG 2 cut(s) 24, 749
MaeII ACGT 1 cut(s) 259
MaeIII GTNAC 5 cut(s) 35, 281, 290, 459, 615
MalI GATC 8 cut(s) 6, 267, 734, 758, 945, 1149, 1220, 1299
MboI GATC 8 cut(s) 4, 265, 732, 756, 943, 1147, 1218, 1297
MboII GAAGA 5 cut(s) 257, 616, 1112, 1210, 1380
MfeI CAATTG 3 cut(s) 1007, 1193, 1303
MflI RGATCY 1 cut(s) 1218
MhlI GDGCHC 2 cut(s) 926, 1107
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 200
Mph1103I ATGCAT 1 cut(s) 1215
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 6 cut(s) 327, 594, 659, 911, 1206, 1344
MspI CCGG 2 cut(s) 68, 1438
MspR9I CCNGG 2 cut(s) 41, 1357
MunI CAATTG 3 cut(s) 1007, 1193, 1303
Mva1269I GAATGC 2 cut(s) 75, 586
MvaI CCWGG 2 cut(s) 41, 1357
NcoI CCATGG 1 cut(s) 338
NdeII GATC 8 cut(s) 4, 265, 732, 756, 943, 1147, 1218, 1297
NlaIII CATG 5 cut(s) 133, 342, 350, 675, 1217
NlaIV GGNNCC 3 cut(s) 336, 1166, 1220
NmuCI GTSAC 2 cut(s) 290, 615
NsiI ATGCAT 1 cut(s) 1215
PciSI GCTCTTC 1 cut(s) 1095
PctI GAATGC 2 cut(s) 75, 586
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 4 cut(s) 83, 558, 1179, 1258
PflMI CCANNNNNTGG 1 cut(s) 992
PinAI ACCGGT 1 cut(s) 1437
PkrI GCNGC 2 cut(s) 345, 980
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
PshBI ATTAAT 1 cut(s) 911
Psp124BI GAGCTC 1 cut(s) 1107
Psp6I CCWGG 2 cut(s) 39, 1355
PspGI CCWGG 2 cut(s) 39, 1355
PspN4I GGNNCC 3 cut(s) 336, 1166, 1220
PspPI GGNCC 3 cut(s) 428, 1072, 1443
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PstNI CAGNNNCTG 1 cut(s) 463
PsuI RGATCY 1 cut(s) 1218
RsaI GTAC 2 cut(s) 680, 742
RsaNI GTAC 2 cut(s) 679, 741
SacI GAGCTC 1 cut(s) 1107
SapI GCTCTTC 1 cut(s) 1095
SaqAI TTAA 6 cut(s) 327, 594, 659, 911, 1206, 1344
SatI GCNGC 2 cut(s) 344, 979
Sau3AI GATC 8 cut(s) 4, 265, 732, 756, 943, 1147, 1218, 1297
Sau96I GGNCC 3 cut(s) 428, 1072, 1443
ScaI AGTACT 1 cut(s) 680
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 41, 1357
SduI GDGCHC 2 cut(s) 926, 1107
SfaNI GCATC 4 cut(s) 103, 1053, 1200, 1405
SfcI CTRYAG 1 cut(s) 947
SinI GGWCC 3 cut(s) 428, 1072, 1443
SmlI CTYRAG 4 cut(s) 932, 1001, 1079, 1280
SmoI CTYRAG 4 cut(s) 932, 1001, 1079, 1280
SsiI CCGC 1 cut(s) 104
SspI AATATT 1 cut(s) 700
SspMI CTAG 2 cut(s) 24, 749
SstI GAGCTC 1 cut(s) 1107
StyD4I CCNGG 2 cut(s) 39, 1355
StyI CCWWGG 4 cut(s) 286, 338, 1222, 1318
TaaI ACNGT 1 cut(s) 465
TaiI ACGT 1 cut(s) 262
TaqI TCGA 2 cut(s) 759, 1420
TatI WGTACW 2 cut(s) 678, 740
TfiI GAWTC 4 cut(s) 83, 558, 1179, 1258
Tru1I TTAA 6 cut(s) 327, 594, 659, 911, 1206, 1344
Tru9I TTAA 6 cut(s) 327, 594, 659, 911, 1206, 1344
TseFI GTSAC 2 cut(s) 290, 615
TseI GCWGC 2 cut(s) 343, 978
Tsp45I GTSAC 2 cut(s) 290, 615
TspDTI ATGAA 4 cut(s) 84, 258, 363, 1043
TspGWI ACGGA 3 cut(s) 228, 570, 796
Van91I CCANNNNNTGG 1 cut(s) 992
VpaK11BI GGWCC 3 cut(s) 428, 1072, 1443
VspI ATTAAT 1 cut(s) 911
XagI CCTNNNNNAGG 1 cut(s) 1079
XapI RAATTY 5 cut(s) 163, 421, 548, 606, 1428
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 2 cut(s) 24, 749
ZrmI AGTACT 1 cut(s) 680
Zsp2I ATGCAT 1 cut(s) 1215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.