Rh5BG065900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018000
Physical Location & Seq
Forward (+)
5439421 .. 5441043
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG065900.1

Sequence Viewer

Length: 552 bp
ATGGCATCCCGTCTGCAAAACAAGATTTCTGTAGATGTTGCAGCTGATAAGAGTTCAAGCGATTCCTTTTCGTTTGCTGGTCTCCTGTGTGTTCAGGACCAGCAAGCGAAGTCACTTCAATGTAACAGAGCCAATCAAATTCACAAACAAGGCACAGAGTTTGAGTTTAGCACTATAGAAGAGTCCTTTGTGTGTGATCCAATCAAGCATTGCCCAGCTGATCTGTTAATCTCAAACGGTCAGATTGTACCACAACCAATCAAATTTCAACCAACACAGCATACTGTGACCAAGCAGCAACACAAGAAAACTTCAGTGCGAGCAACCAGTACTAGTAGCAAGAGATCAACTCAAAATACCAGTGGCACCCAAGCTTCTGCAAAACCATATGCAGAGCATAATGACCAAGACAAGAAGGAGCATGCTGTGCCAAGCTCATGGTTTGGCCGGAAAATATTCAAGTCATTTTTCTTCCCTTGCAAGGAAAGCAAAGCTGTTAAGCCAACCCTGAGAGCAAATAGAATGCCAGAAGCAAATGGGAAGCTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.38

Weight (kDa)

9.54

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018000)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08820
prunus_persica Prupe.4G050900_v2.0.a1
pyrus_communis pycom10g24350
rosa_laevigata RLG00000031618
rosa_multiflora Rmu_sc0004677.1_g000019
rosa_roxburghii Rroxscaffold_1G00067080
rosa_rugosa Rorug04G0440400
rosa_samantha Rh5AG069100 Rh5BG065900 Rh5CG077100 Rh5DG064900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 365
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 2 cut(s) 138, 263
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 2 cut(s) 249, 331
AfiI CCNNNNNNNGG 1 cut(s) 481
AgsI TTSAA 4 cut(s) 57, 119, 269, 460
AhlI ACTAGT 1 cut(s) 332
AluBI AGCT 6 cut(s) 44, 218, 374, 435, 494, 544
AluI AGCT 6 cut(s) 44, 218, 374, 435, 494, 544
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 191
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 2 cut(s) 41, 295
ApoI RAATTY 2 cut(s) 138, 263
Asp700I GAANNNNTTC 1 cut(s) 455
AspS9I GGNCC 1 cut(s) 97
AvaII GGWCC 1 cut(s) 97
BanI GGYRCC 1 cut(s) 365
BbvI GCAGC 2 cut(s) 53, 307
BcoDI GTCTC 1 cut(s) 86
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 2 cut(s) 30, 174
BisI GCNGC 2 cut(s) 42, 296
BlsI GCNGC 2 cut(s) 43, 297
BmcAI AGTACT 1 cut(s) 331
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 1 cut(s) 367
BmsI GCATC 1 cut(s) 14
BplI GAGNNNNNCTC 2 cut(s) 334, 366
BsaI GGTCTC 1 cut(s) 86
Bsc4I CCNNNNNNNGG 1 cut(s) 481
Bse1I ACTGG 2 cut(s) 327, 360
Bse3DI GCAATG 1 cut(s) 208
BseGI GGATG 1 cut(s) 5
BseLI CCNNNNNNNGG 1 cut(s) 481
BseMI GCAATG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 2 cut(s) 327, 360
BseXI GCAGC 2 cut(s) 53, 307
BseYI CCCAGC 1 cut(s) 214
BshFI GGCC 1 cut(s) 447
BshNI GGYRCC 1 cut(s) 365
BsiSI CCGG 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 481
BsmAI GTCTC 1 cut(s) 86
BsmI GAATGC 1 cut(s) 528
BsnI GGCC 1 cut(s) 447
Bso31I GGTCTC 1 cut(s) 86
Bsp143I GATC 3 cut(s) 196, 220, 344
BspANI GGCC 1 cut(s) 447
BspCNI CTCAG 1 cut(s) 501
BspLI GGNNCC 1 cut(s) 367
BspPI GGATC 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 365
BspTNI GGTCTC 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 208
BsrI ACTGG 2 cut(s) 327, 360
BssMI GATC 3 cut(s) 196, 220, 344
Bst4CI ACNGT 2 cut(s) 239, 286
Bst6I CTCTTC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 427
BstC8I GCNNGC 3 cut(s) 105, 321, 423
BstDEI CTNAG 1 cut(s) 509
BstF5I GGATG 1 cut(s) 5
BstKTI GATC 3 cut(s) 199, 223, 347
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 3 cut(s) 196, 220, 344
BstMWI GCNNNNNNNGC 2 cut(s) 427, 486
BstNSI RCATGY 1 cut(s) 425
BstSFI CTRYAG 2 cut(s) 30, 174
BstV1I GCAGC 2 cut(s) 53, 307
BstXI CCANNNNNNTGG 1 cut(s) 438
BsuRI GGCC 1 cut(s) 447
BtsCI GGATG 1 cut(s) 5
BtsIMutI CAGTG 2 cut(s) 321, 367
Cac8I GCNNGC 3 cut(s) 105, 321, 423
Cfr13I GGNCC 1 cut(s) 97
Csp6I GTAC 2 cut(s) 248, 330
CviAII CATG 2 cut(s) 422, 438
CviJI RGCY 9 cut(s) 44, 131, 218, 374, 435, 447, 494, 502, 544
CviKI_1 RGCY 9 cut(s) 44, 131, 218, 374, 435, 447, 494, 502, 544
CviQI GTAC 2 cut(s) 248, 330
DdeI CTNAG 1 cut(s) 509
DpnI GATC 3 cut(s) 198, 222, 346
DpnII GATC 3 cut(s) 196, 220, 344
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 297
FaeI CATG 2 cut(s) 425, 441
FaiI YATR 7 cut(s) 176, 282, 388, 390, 399, 423, 439
FatI CATG 2 cut(s) 421, 437
FauNDI CATATG 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 42, 296
Fsp4HI GCNGC 2 cut(s) 42, 296
FspBI CTAG 1 cut(s) 333
GluI GCNGC 2 cut(s) 42, 296
GsaI CCCAGC 1 cut(s) 218
HaeIII GGCC 1 cut(s) 447
HapII CCGG 1 cut(s) 448
Hin1II CATG 2 cut(s) 425, 441
HindIII AAGCTT 1 cut(s) 372
HinfI GANTC 2 cut(s) 62, 182
HpaII CCGG 1 cut(s) 448
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 1 cut(s) 95
HpyAV CCTTC 1 cut(s) 409
HpyCH4III ACNGT 2 cut(s) 239, 286
HpyCH4V TGCA 5 cut(s) 16, 41, 380, 392, 480
HpyF10VI GCNNNNNNNGC 2 cut(s) 427, 486
HpyF3I CTNAG 1 cut(s) 509
Hsp92II CATG 2 cut(s) 425, 441
Kzo9I GATC 3 cut(s) 196, 220, 344
LmnI GCTCC 1 cut(s) 418
Lsp1109I GCAGC 2 cut(s) 53, 307
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 111, 122, 286
MalI GATC 3 cut(s) 198, 222, 346
MboI GATC 3 cut(s) 196, 220, 344
MboII GAAGA 2 cut(s) 191, 463
MluCI AATT 2 cut(s) 138, 263
MlyI GAGTC 1 cut(s) 191
MroXI GAANNNNTTC 1 cut(s) 455
MseI TTAA 2 cut(s) 227, 498
MslI CAYNNNNRTG 1 cut(s) 118
MspA1I CMGCKG 2 cut(s) 44, 218
MspI CCGG 1 cut(s) 448
Mva1269I GAATGC 1 cut(s) 528
MwoI GCNNNNNNNGC 2 cut(s) 427, 486
NdeI CATATG 1 cut(s) 388
NdeII GATC 3 cut(s) 196, 220, 344
NlaIII CATG 2 cut(s) 425, 441
NlaIV GGNNCC 1 cut(s) 367
NmuCI GTSAC 2 cut(s) 111, 286
NspI RCATGY 1 cut(s) 425
PaeI GCATGC 1 cut(s) 425
PctI GAATGC 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 455
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 43, 297
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 214
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 1 cut(s) 97
PvuII CAGCTG 2 cut(s) 44, 218
RsaI GTAC 2 cut(s) 249, 331
RsaNI GTAC 2 cut(s) 248, 330
RseI CAYNNNNRTG 1 cut(s) 118
SaqAI TTAA 2 cut(s) 227, 498
SatI GCNGC 2 cut(s) 42, 296
Sau3AI GATC 3 cut(s) 196, 220, 344
Sau96I GGNCC 1 cut(s) 97
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 1 cut(s) 191
SetI ASST 6 cut(s) 46, 220, 376, 437, 496, 546
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 2 cut(s) 30, 174
SinI GGWCC 1 cut(s) 97
SmiMI CAYNNNNRTG 1 cut(s) 118
SpeI ACTAGT 1 cut(s) 332
SphI GCATGC 1 cut(s) 425
Sse9I AATT 2 cut(s) 138, 263
SspI AATATT 1 cut(s) 456
SspMI CTAG 1 cut(s) 333
TaaI ACNGT 2 cut(s) 239, 286
TasI AATT 2 cut(s) 138, 263
TatI WGTACW 1 cut(s) 329
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 2 cut(s) 227, 498
Tru9I TTAA 2 cut(s) 227, 498
TscAI CASTG 2 cut(s) 321, 367
TseFI GTSAC 2 cut(s) 111, 286
TseI GCWGC 2 cut(s) 41, 295
Tsp45I GTSAC 2 cut(s) 111, 286
TspRI CASTG 2 cut(s) 321, 367
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 2 cut(s) 138, 263
XceI RCATGY 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 455
XspI CTAG 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.