Rh5BG123900

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018126
Physical Location & Seq
Forward (+)
11726695 .. 11728378
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG123900.1

Sequence Viewer

Length: 1158 bp
ATGGGTTTAGGTTTGTTTTTAAACATCAATACAGTGGAAGAAGCAAGGGATGCATTGGATTCACTACTTGAAAAACTCAAAGATTCTTGCTTGTTGATGGACAGTGATGTTGATGGCACGGTCAGAATGCATGATCTTGTATGTCATGTTGCTAACCGGATTGCAAAGAGAGATCAACACATGTTATCAGTAGAGTATGGAGGTGAGTTGAAAGAATGGCCAAGTAAGGATTTCTTAAAGAAGTGCACTAAGATCTCTCTCCTGCAAGTCAATATTCCTACACTTTCTGAAATGCCTTTGGAATGTCCAGAATTAGAATTGTTTCACTTGTACAATAAAGATTTCTCGATGGAAATCCCATCCAACTTCTTTAGAGAGATGCAGAAACTCAAAGTTCTGGATTTAACCAACATGTATATGCCATCATTGCCTTTATCTCTTCAATACTTGAAAACGCTTCAAACGTTGTGCTTAGATGGGTGCATGCTGGGAGATATAACTCTACTCGGGCAGCTAAGCAGCTTAGAAATTCTTAGCCTTTTACAATCCAATTTTGAAGAATTGCCTAGAGAAATAGGGCAATTGACTCGTCTTCGGTTATTGGATTTATCCGAGTGTTATCAACTTGAAGTGATTTCACCTACTGTCATATCAAGCTTGGTGAGATTGGAAGATTTGAGAATGAGAAACAGCTTTAACAAATGGGAGGTTGAAGGTGAAAGTAGTAATGCAAGCCTGTCTGAGCTAAAGCACTTGTCTCAGCTATCCGCATTAGAAATACATATTCCGAATGCTGACATTCTTCCAGCAAACTTGTTCTCCCACAAATTAGAGAGATATAATATTTTTATTGGAGACGCATGGGGTATGGGATATTCTATGGCTAGAACGAGCCTTAACAAACTGAAACTCAAGCTCACTAGCAACAATATTATGGGATTGGATGAAGGCCTAAAATCGTTGCTGAAGAGAGCTGAGGACTTGTCCCTAGATGAGAGTATAGTTAATATTGTCCGGCAATTGGATACCAAAGATTTTGAGAACGTGAAACATCTCCGACTCCAAAACATCTTTGACTTTACAAATATCATTGATAGAAAGGTATTTTCCTGTCTTTTTATGTACTTTTCTATGACCGTTTCATTTTATAATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

385

Amino Acids

44.19

Weight (kDa)

5.19

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 169 - 264 8.7e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1149
AciI CCGC 1 cut(s) 768
AclI AACGTT 1 cut(s) 464
AcoI YGGCCR 1 cut(s) 218
AcsI RAATTY 1 cut(s) 528
AcuI CTGAAG 1 cut(s) 986
AfaI GTAC 2 cut(s) 332, 1124
AfiI CCNNNNNNNGG 1 cut(s) 1021
AflIII ACRYGT 2 cut(s) 180, 411
AgsI TTSAA 8 cut(s) 71, 211, 443, 451, 461, 557, 629, 713
AluBI AGCT 8 cut(s) 514, 522, 657, 693, 745, 763, 916, 974
AluI AGCT 8 cut(s) 514, 522, 657, 693, 745, 763, 916, 974
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 2 cut(s) 762, 849
Alw44I GTGCAC 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 506
AoxI GGCC 2 cut(s) 218, 949
ApaLI GTGCAC 1 cut(s) 244
ApeKI GCWGC 2 cut(s) 511, 519
ApoI RAATTY 1 cut(s) 528
Asp700I GAANNNNTTC 1 cut(s) 321
AsuHPI GGTGA 4 cut(s) 215, 630, 673, 728
AvaI CYCGRG 1 cut(s) 506
BaeGI GKGCMC 1 cut(s) 248
BalI TGGCCA 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 584
Bbv12I GWGCWC 1 cut(s) 248
BbvCI CCTCAGC 1 cut(s) 975
BbvI GCAGC 2 cut(s) 523, 531
BccI CCATC 6 cut(s) 91, 107, 343, 367, 430, 470
BcgI CGANNNNNNTGC 2 cut(s) 569, 603
BciVI GTATCC 1 cut(s) 1018
BcoDI GTCTC 2 cut(s) 762, 849
BfaI CTAG 4 cut(s) 567, 885, 921, 989
BfuI GTATCC 1 cut(s) 1018
BglII AGATCT 1 cut(s) 252
BisI GCNGC 2 cut(s) 512, 520
BlpI GCTNAGC 1 cut(s) 515
BlsI GCNGC 2 cut(s) 513, 521
BmeT110I CYCGRG 1 cut(s) 506
BmsI GCATC 2 cut(s) 40, 369
BpiI GAAGAC 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 975
Bpu1102I GCTNAGC 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 896
BsaBI GATNNNNATC 1 cut(s) 353
BsaWI WCCGGW 1 cut(s) 156
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 1 cut(s) 1021
Bse3DI GCAATG 1 cut(s) 425
Bse8I GATNNNNATC 1 cut(s) 353
BseGI GGATG 3 cut(s) 55, 359, 949
BseJI GATNNNNATC 1 cut(s) 353
BseLI CCNNNNNNNGG 1 cut(s) 1021
BseMI GCAATG 1 cut(s) 425
BseMII CTCAG 3 cut(s) 732, 773, 966
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 2 cut(s) 523, 531
BseYI CCCAGC 1 cut(s) 487
BshFI GGCC 2 cut(s) 220, 951
BsiHKAI GWGCWC 1 cut(s) 248
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 2 cut(s) 157, 1015
BslFI GGGAC 1 cut(s) 970
BslI CCNNNNNNNGG 1 cut(s) 1021
BsmAI GTCTC 2 cut(s) 762, 849
BsmBI CGTCTC 1 cut(s) 849
BsmFI GGGAC 1 cut(s) 970
BsmI GAATGC 2 cut(s) 132, 796
BsnI GGCC 2 cut(s) 220, 951
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 248
Bsp1407I TGTACA 1 cut(s) 330
Bsp143I GATC 3 cut(s) 133, 172, 252
Bsp1720I GCTNAGC 1 cut(s) 515
BspACI CCGC 1 cut(s) 768
BspANI GGCC 2 cut(s) 220, 951
BspCNI CTCAG 3 cut(s) 733, 772, 967
BsrDI GCAATG 1 cut(s) 425
BsrGI TGTACA 1 cut(s) 330
BssMI GATC 3 cut(s) 133, 172, 252
Bst4CI ACNGT 5 cut(s) 34, 104, 121, 646, 1138
Bst6I CTCTTC 2 cut(s) 444, 962
BstAPI GCANNNNNTGC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 485, 733
BstDEI CTNAG 8 cut(s) 249, 472, 515, 523, 533, 741, 759, 975
BstF5I GGATG 3 cut(s) 55, 359, 949
BstKTI GATC 3 cut(s) 136, 175, 255
BstMAI GTCTC 2 cut(s) 762, 849
BstMBI GATC 3 cut(s) 133, 172, 252
BstMWI GCNNNNNNNGC 2 cut(s) 50, 427
BstNSI RCATGY 3 cut(s) 184, 415, 487
BstSLI GKGCMC 1 cut(s) 248
BstV1I GCAGC 2 cut(s) 523, 531
BstV2I GAAGAC 1 cut(s) 584
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BsuI GTATCC 1 cut(s) 1018
BsuRI GGCC 2 cut(s) 220, 951
BtsCI GGATG 3 cut(s) 55, 359, 949
BtsIMutI CAGTG 2 cut(s) 39, 109
Cac8I GCNNGC 2 cut(s) 485, 733
CseI GACGC 1 cut(s) 866
Csp6I GTAC 2 cut(s) 331, 1123
CviAII CATG 6 cut(s) 131, 146, 181, 412, 484, 861
CviQI GTAC 2 cut(s) 331, 1123
DdeI CTNAG 8 cut(s) 249, 472, 515, 523, 533, 741, 759, 975
DpnI GATC 3 cut(s) 135, 174, 254
DpnII GATC 3 cut(s) 133, 172, 252
DraI TTTAAA 1 cut(s) 21
EaeI YGGCCR 1 cut(s) 218
Eam1104I CTCTTC 2 cut(s) 444, 962
EarI CTCTTC 2 cut(s) 444, 962
Eco147I AGGCCT 1 cut(s) 951
Eco57I CTGAAG 1 cut(s) 986
Eco88I CYCGRG 1 cut(s) 506
EcoT22I ATGCAT 2 cut(s) 55, 132
Esp3I CGTCTC 1 cut(s) 849
FaeI CATG 6 cut(s) 134, 149, 184, 415, 487, 864
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 970
FatI CATG 6 cut(s) 130, 145, 180, 411, 483, 860
Fnu4HI GCNGC 2 cut(s) 512, 520
FokI GGATG 3 cut(s) 62, 346, 956
Fsp4HI GCNGC 2 cut(s) 512, 520
FspBI CTAG 4 cut(s) 567, 885, 921, 989
GluI GCNGC 2 cut(s) 512, 520
GsaI CCCAGC 1 cut(s) 491
HaeIII GGCC 2 cut(s) 220, 951
HapII CCGG 2 cut(s) 157, 1015
HgaI GACGC 1 cut(s) 866
Hin1II CATG 6 cut(s) 134, 149, 184, 415, 487, 864
HindIII AAGCTT 1 cut(s) 655
HinfI GANTC 4 cut(s) 59, 83, 586, 1059
HpaII CCGG 2 cut(s) 157, 1015
HphI GGTGA 4 cut(s) 215, 630, 673, 728
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 6 cut(s) 125, 289, 613, 742, 789, 1058
Hpy188III TCNNGA 3 cut(s) 308, 346, 398
Hpy8I GTNNAC 1 cut(s) 246
HpyAV CCTTC 2 cut(s) 707, 941
HpyCH4III ACNGT 5 cut(s) 34, 104, 121, 646, 1138
HpyCH4IV ACGT 2 cut(s) 464, 1044
HpyCH4V TGCA 8 cut(s) 53, 130, 164, 246, 265, 382, 483, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 427
HpyF3I CTNAG 8 cut(s) 249, 472, 515, 523, 533, 741, 759, 975
HpySE526I ACGT 2 cut(s) 464, 1044
Hsp92II CATG 6 cut(s) 134, 149, 184, 415, 487, 864
Kzo9I GATC 3 cut(s) 133, 172, 252
LpnPI CCDG 9 cut(s) 170, 275, 321, 383, 473, 749, 819, 1028, 1123
Lsp1109I GCAGC 2 cut(s) 523, 531
LweI GCATC 2 cut(s) 40, 369
MaeI CTAG 4 cut(s) 567, 885, 921, 989
MaeII ACGT 2 cut(s) 464, 1044
MalI GATC 3 cut(s) 135, 174, 254
MboI GATC 3 cut(s) 133, 172, 252
MboII GAAGA 7 cut(s) 50, 431, 569, 584, 683, 794, 979
MfeI CAATTG 2 cut(s) 581, 1019
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 248
MlsI TGGCCA 1 cut(s) 220
MluCI AATT 8 cut(s) 311, 317, 528, 550, 560, 581, 827, 1019
MluNI TGGCCA 1 cut(s) 220
MlyI GAGTC 2 cut(s) 580, 1053
MmeI TCCRAC 2 cut(s) 387, 1081
MnlI CCTC 3 cut(s) 194, 700, 970
Mox20I TGGCCA 1 cut(s) 220
Mph1103I ATGCAT 2 cut(s) 55, 132
MroXI GAANNNNTTC 1 cut(s) 321
MscI TGGCCA 1 cut(s) 220
MseI TTAA 6 cut(s) 20, 236, 404, 696, 897, 1005
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 220
MspI CCGG 2 cut(s) 157, 1015
MunI CAATTG 2 cut(s) 581, 1019
Mva1269I GAATGC 2 cut(s) 132, 796
MwoI GCNNNNNNNGC 2 cut(s) 50, 427
NdeII GATC 3 cut(s) 133, 172, 252
NlaIII CATG 6 cut(s) 134, 149, 184, 415, 487, 864
NsiI ATGCAT 2 cut(s) 55, 132
NspI RCATGY 3 cut(s) 184, 415, 487
PaeI GCATGC 1 cut(s) 487
PceI AGGCCT 1 cut(s) 951
PciI ACATGT 2 cut(s) 180, 411
PcsI WCGNNNNNNNCGW 1 cut(s) 461
PctI GAATGC 2 cut(s) 132, 796
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 59, 83
PkrI GCNGC 2 cut(s) 513, 521
PleI GAGTC 2 cut(s) 580, 1053
PpsI GAGTC 2 cut(s) 580, 1053
PscI ACATGT 2 cut(s) 180, 411
PsiI TTATAA 1 cut(s) 1149
Psp1406I AACGTT 1 cut(s) 464
PspFI CCCAGC 1 cut(s) 487
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 2 cut(s) 332, 1124
RsaNI GTAC 2 cut(s) 331, 1123
RseI CAYNNNNRTG 1 cut(s) 416
SaqAI TTAA 6 cut(s) 20, 236, 404, 696, 897, 1005
SatI GCNGC 2 cut(s) 512, 520
Sau3AI GATC 3 cut(s) 133, 172, 252
SchI GAGTC 2 cut(s) 580, 1053
SduI GDGCHC 1 cut(s) 248
SfaNI GCATC 2 cut(s) 40, 369
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
SphI GCATGC 1 cut(s) 487
Sse9I AATT 8 cut(s) 311, 317, 528, 550, 560, 581, 827, 1019
SseBI AGGCCT 1 cut(s) 951
SsiI CCGC 1 cut(s) 768
SspI AATATT 4 cut(s) 274, 844, 931, 1009
SspMI CTAG 4 cut(s) 567, 885, 921, 989
StuI AGGCCT 1 cut(s) 951
TaaI ACNGT 5 cut(s) 34, 104, 121, 646, 1138
TaiI ACGT 2 cut(s) 467, 1047
TaqI TCGA 1 cut(s) 347
TasI AATT 8 cut(s) 311, 317, 528, 550, 560, 581, 827, 1019
TatI WGTACW 2 cut(s) 330, 1122
TfiI GAWTC 2 cut(s) 59, 83
Tru1I TTAA 6 cut(s) 20, 236, 404, 696, 897, 1005
Tru9I TTAA 6 cut(s) 20, 236, 404, 696, 897, 1005
TscAI CASTG 2 cut(s) 39, 109
TseI GCWGC 2 cut(s) 511, 519
TspDTI ATGAA 2 cut(s) 960, 1131
TspRI CASTG 2 cut(s) 39, 109
VneI GTGCAC 1 cut(s) 244
XapI RAATTY 1 cut(s) 528
XceI RCATGY 3 cut(s) 184, 415, 487
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 4 cut(s) 567, 885, 921, 989
Zsp2I ATGCAT 2 cut(s) 55, 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.