Rh5CG303400

Ubiquitin thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009522
Physical Location & Seq
Reverse (-)
33400358 .. 33404720
4363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG303400.1

Sequence Viewer

Length: 495 bp
ATGGAAGGTACAGTCGTTAGAAGAGTTATTCCCTCAGACAATAGTTGCCTCTTCAATGCAGTTGGGTATGTTATGGACCATGACAAGCAAAAAGCTGCTGAATTGAGACAGGTTATAGCCGCAACGGTGGCAAGTGATCCAACTAAATATTCTGAAGCAATTCTGGGTAAACCAAATGAAGAGTATTGTGCTTGGATTCTCAACCCAGAGAAGTGGGGAGGTGCTATCGAGCTTTCAATATTAGCAGATTATTATGGACGTGAAATTGCAGCATATGATATCCAGACTACAAGATGCGATTTGTATGGTCAGGAGAAAAACTATTCTGAAAGGGTTATGCTGATCTATGATGGGCTTCACTACGATGCTTTAGCTATGTCTCCATTTGAAGAAGCCCCGGAGGAGTTTGATCAGACTATATTTGCAGTTAAGAACAAGACCATTGGGCCAGTGGAGGGCATCACTCTTAATCTTGTGAAAGAGCAACAACGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
OTU

Protein Analysis

164

Amino Acids

18.49

Weight (kDa)

4.66

Isoelectric Point (pI)

56.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 11 - 113 2e-09 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 120
AclWI GGATC 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 455
AgsI TTSAA 3 cut(s) 55, 237, 389
AjiI CACGTC 1 cut(s) 260
AjuI GAANNNNNNNTTGG 2 cut(s) 133, 165
AluBI AGCT 3 cut(s) 95, 232, 374
AluI AGCT 3 cut(s) 95, 232, 374
Alw26I GTCTC 2 cut(s) 100, 384
AlwI GGATC 1 cut(s) 131
AoxI GGCC 1 cut(s) 446
ApeKI GCWGC 2 cut(s) 95, 269
Asp700I GAANNNNTTC 1 cut(s) 159
AspS9I GGNCC 2 cut(s) 76, 446
AsuC2I CCSGG 1 cut(s) 398
AvaII GGWCC 1 cut(s) 76
BaeI ACNNNNGTAYC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 82, 281
BccI CCATC 1 cut(s) 344
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 398
BcoDI GTCTC 2 cut(s) 100, 384
BisI GCNGC 3 cut(s) 96, 120, 270
BlsI GCNGC 3 cut(s) 97, 121, 271
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 76, 446
BmrFI CCNGG 1 cut(s) 398
BmsI GCATC 3 cut(s) 284, 355, 468
BpuMI CCSGG 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 396
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse1I ACTGG 1 cut(s) 449
BseDI CCNNGG 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 449
BseRI GAGGAG 1 cut(s) 416
BseXI GCAGC 2 cut(s) 82, 281
BshFI GGCC 1 cut(s) 448
BsiSI CCGG 1 cut(s) 398
BslI CCNNNNNNNGG 1 cut(s) 455
BsmAI GTCTC 2 cut(s) 100, 384
BsnI GGCC 1 cut(s) 448
Bsp143I GATC 3 cut(s) 136, 342, 409
BspACI CCGC 1 cut(s) 120
BspANI GGCC 1 cut(s) 448
BspCNI CTCAG 1 cut(s) 47
BspPI GGATC 1 cut(s) 131
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 1 cut(s) 396
BssMI GATC 3 cut(s) 136, 342, 409
Bst4CI ACNGT 3 cut(s) 13, 127, 492
Bst6I CTCTTC 3 cut(s) 16, 56, 174
BstDEI CTNAG 1 cut(s) 34
BstKTI GATC 3 cut(s) 139, 345, 412
BstMAI GTCTC 2 cut(s) 100, 384
BstMBI GATC 3 cut(s) 136, 342, 409
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 396
BstV1I GCAGC 2 cut(s) 82, 281
BstXI CCANNNNNNTGG 1 cut(s) 213
BsuRI GGCC 1 cut(s) 448
BtrI CACGTC 1 cut(s) 260
BtsIMutI CAGTG 1 cut(s) 456
Cfr13I GGNCC 2 cut(s) 76, 446
Csp6I GTAC 1 cut(s) 9
CviAII CATG 1 cut(s) 80
CviJI RGCY 7 cut(s) 95, 119, 232, 355, 374, 395, 448
CviKI_1 RGCY 7 cut(s) 95, 119, 232, 355, 374, 395, 448
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 1 cut(s) 34
DpnI GATC 3 cut(s) 138, 344, 411
DpnII GATC 3 cut(s) 136, 342, 409
Eam1104I CTCTTC 3 cut(s) 16, 56, 174
EarI CTCTTC 3 cut(s) 16, 56, 174
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 76
Eco57I CTGAAG 1 cut(s) 174
EcoRV GATATC 1 cut(s) 280
FaeI CATG 1 cut(s) 83
FatI CATG 1 cut(s) 79
FauNDI CATATG 1 cut(s) 274
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 96, 120, 270
Fsp4HI GCNGC 3 cut(s) 96, 120, 270
GluI GCNGC 3 cut(s) 96, 120, 270
HaeIII GGCC 1 cut(s) 448
HapII CCGG 1 cut(s) 398
Hin1II CATG 1 cut(s) 83
HinfI GANTC 1 cut(s) 196
HpaII CCGG 1 cut(s) 398
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 4 cut(s) 37, 154, 328, 414
Hpy188III TCNNGA 2 cut(s) 283, 311
Hpy8I GTNNAC 1 cut(s) 170
HpyCH4III ACNGT 3 cut(s) 13, 127, 492
HpyCH4IV ACGT 1 cut(s) 259
HpyCH4V TGCA 3 cut(s) 59, 269, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 1 cut(s) 34
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 3 cut(s) 136, 342, 409
LpnPI CCDG 7 cut(s) 95, 149, 219, 296, 296, 411, 462
Lsp1109I GCAGC 2 cut(s) 82, 281
LweI GCATC 3 cut(s) 284, 355, 468
MaeII ACGT 1 cut(s) 259
MalI GATC 3 cut(s) 138, 344, 411
MboI GATC 3 cut(s) 136, 342, 409
MboII GAAGA 4 cut(s) 33, 43, 191, 401
MluCI AATT 3 cut(s) 101, 159, 264
MmeI TCCRAC 1 cut(s) 164
MnlI CCTC 5 cut(s) 43, 59, 212, 394, 448
MroXI GAANNNNTTC 1 cut(s) 159
MseI TTAA 2 cut(s) 429, 468
MslI CAYNNNNRTG 1 cut(s) 363
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 1 cut(s) 398
MwoI GCNNNNNNNGC 1 cut(s) 128
NciI CCSGG 1 cut(s) 398
NdeI CATATG 1 cut(s) 274
NdeII GATC 3 cut(s) 136, 342, 409
NlaIII CATG 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 196
PkrI GCNGC 3 cut(s) 97, 121, 271
PspPI GGNCC 2 cut(s) 76, 446
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 363
SaqAI TTAA 2 cut(s) 429, 468
SatI GCNGC 3 cut(s) 96, 120, 270
Sau3AI GATC 3 cut(s) 136, 342, 409
Sau96I GGNCC 2 cut(s) 76, 446
ScrFI CCNGG 1 cut(s) 398
SetI ASST 7 cut(s) 10, 97, 114, 223, 234, 262, 376
SfaNI GCATC 3 cut(s) 284, 355, 468
SinI GGWCC 1 cut(s) 76
SmiMI CAYNNNNRTG 1 cut(s) 363
Sse9I AATT 3 cut(s) 101, 159, 264
SsiI CCGC 1 cut(s) 120
SspI AATATT 2 cut(s) 149, 240
StyD4I CCNGG 1 cut(s) 396
TaaI ACNGT 3 cut(s) 13, 127, 492
TaiI ACGT 1 cut(s) 262
TaqI TCGA 1 cut(s) 228
TasI AATT 3 cut(s) 101, 159, 264
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 2 cut(s) 429, 468
Tru9I TTAA 2 cut(s) 429, 468
TscAI CASTG 1 cut(s) 456
TseI GCWGC 2 cut(s) 95, 269
TspDTI ATGAA 1 cut(s) 192
TspRI CASTG 1 cut(s) 456
VpaK11BI GGWCC 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XmnI GAANNNNTTC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.