Rh5DG083200
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015786
Physical Location & Seq
Forward (+)
7326727 .. 7329147
2421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG083200.1

Sequence Viewer

Length: 801 bp
ATGGAATATGTTCCGGAGACCATGTATCAGGTCTTGAAGCATTACAGCAATGCAGACCAGAGAATGCCCCTTGTCTATGTCAAGCTTTACATGTACCAGATTTTCAGAGGACTGGCTTACATACACACCGTTCCTGGAGTTTGCCATAGAGATTTGAAGCCTCAAAATTTATTGGTCGATCCTCTAACTCACCAGGTCAAGCTCTGTGATTTTGGAAGTGCCAAAATGCTGGTGAAAGGTGAATCAAACATAGCATACATATGTTCACGTTTCTATCGGGCTCCAGAACTAATATTTGGTGCCACGGATTATACAACTTCAATTGATATCTGGTCAGCTGGCTGTGTCCTAGCAGAGCTTCTTTTGGGGCAGCCTTTGTTTCCTGGGGAAAATGCAGTGGACCAACTTGTAGAGATCATCAAGGTTCTGGGAACACCAACACGTGAAGAAATTCGCTGTATGAATCCAAATTATACAGATTTCAGGTTTCCACAGATAAAAGCACACCCTTGGCACAAGGTTTTCCACAAGCGCATGCCTCCAGAAGCAATTGATCTGGCTTCAAGACTGCTACAATACTCTCCAAGTCTTCGTTGCACAGCTCTAGAAGCATGTGCACATCCTTTCTTTGACGAACTCCGTGAGTCCAATGCCCGCCTACCAAATGGTCGCCCATTGCCTCCGCTTTTTAATTTTAAGCAAGAGTTGTCCGGCGCTTCTCCAGAGCTTATCAACAAGTTGATTCCTGATCATGTGAAACGCCAAATGGGTCTCCCTTTCTTGCATGCGGCTGGCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009593 GO:0009653 GO:0009719 GO:0009720 GO:0009725 GO:0009726 GO:0009729 GO:0009733 GO:0009741 GO:0009742 GO:0009755 GO:0009825 GO:0009888 GO:0009965 GO:0009966 GO:0009968 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010053 GO:0010054 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023057 GO:0030154 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032880 GO:0033157 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0046822 GO:0046824 GO:0046825 GO:0046827 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048523 GO:0048545 GO:0048583 GO:0048585 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051222 GO:0051223 GO:0051606 GO:0051716 GO:0060341 GO:0065007 GO:0070201 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071695 GO:0071704 GO:0071944 GO:0090087 GO:0090316 GO:0090558 GO:0090627 GO:0099402 GO:0140096 GO:1900457 GO:1900458 GO:1901564 GO:1901700 GO:1901701 GO:1903827 GO:1903829 GO:1904951 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Pkinase

Protein Analysis

266

Amino Acids

30.34

Weight (kDa)

8.12

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 210 4.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 130 6.7e-13 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18710
fragaria_vesca FvH4_3g07480
rosa_chinensis RchiOBHm_Chr5g0011371
rosa_laevigata RLG00000031827
rosa_multiflora Rmu_co8427111.1_g000001 Rmu_sc0009975.1_g000011
rosa_roxburghii Rroxscaffold_1G00065080
rosa_rugosa Rorug04G0456500 Rorug04G0456600 Rorug04G0456600
rosa_samantha Rh5AG088300 Rh5BG083300 Rh5CG096600 Rh5DG083200
rosa_wichuraiana Rw5G007660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 299
AccIII TCCGGA 1 cut(s) 13
AciI CCGC 3 cut(s) 655, 683, 788
AclWI GGATC 1 cut(s) 173
AcsI RAATTY 2 cut(s) 166, 450
AcvI CACGTG 1 cut(s) 443
AfaI GTAC 1 cut(s) 95
AflIII ACRYGT 2 cut(s) 90, 440
AgsI TTSAA 4 cut(s) 37, 157, 321, 564
AjnI CCWGG 3 cut(s) 133, 192, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 6 cut(s) 85, 202, 338, 358, 602, 727
AluI AGCT 6 cut(s) 85, 202, 338, 358, 602, 727
Alw21I GWGCWC 1 cut(s) 619
Alw26I GTCTC 2 cut(s) 11, 776
Alw44I GTGCAC 1 cut(s) 615
AlwI GGATC 1 cut(s) 173
Aor13HI TCCGGA 1 cut(s) 13
ApaLI GTGCAC 1 cut(s) 615
ApeKI GCWGC 1 cut(s) 370
ApoI RAATTY 2 cut(s) 166, 450
Asp700I GAANNNNTTC 2 cut(s) 9, 450
AspLEI GCGC 2 cut(s) 534, 716
AspS9I GGNCC 1 cut(s) 400
AsuHPI GGTGA 3 cut(s) 182, 244, 251
AvaII GGWCC 1 cut(s) 400
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 283
BbrPI CACGTG 1 cut(s) 443
BbsI GAAGAC 1 cut(s) 581
Bbv12I GWGCWC 1 cut(s) 619
BbvI GCAGC 1 cut(s) 382
BciT130I CCWGG 3 cut(s) 135, 194, 384
BclI TGATCA 1 cut(s) 748
BcoDI GTCTC 2 cut(s) 11, 776
BfaI CTAG 2 cut(s) 350, 605
BfoI RGCGCY 1 cut(s) 717
BisI GCNGC 2 cut(s) 371, 789
BlsI GCNGC 2 cut(s) 372, 790
Bme1390I CCNGG 3 cut(s) 135, 194, 384
Bme18I GGWCC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 400
BmiI GGNNCC 2 cut(s) 282, 301
BmrFI CCNGG 3 cut(s) 135, 194, 384
BpiI GAAGAC 1 cut(s) 581
BpmI CTGGAG 4 cut(s) 156, 267, 525, 705
BsaAI YACGTR 1 cut(s) 443
BsaI GGTCTC 2 cut(s) 11, 776
BsaJI CCNNGG 3 cut(s) 303, 383, 509
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bse1I ACTGG 1 cut(s) 117
Bse3DI GCAATG 2 cut(s) 55, 674
BseAI TCCGGA 1 cut(s) 13
BseBI CCWGG 3 cut(s) 135, 194, 384
BseDI CCNNGG 3 cut(s) 303, 383, 509
BseGI GGATG 1 cut(s) 619
BseMI GCAATG 2 cut(s) 55, 674
BseNI ACTGG 1 cut(s) 117
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 1 cut(s) 382
BshNI GGYRCC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 619
BsiSI CCGG 2 cut(s) 14, 711
BsmAI GTCTC 2 cut(s) 11, 776
BsmI GAATGC 1 cut(s) 69
Bso31I GGTCTC 2 cut(s) 11, 776
Bsp1286I GDGCHC 2 cut(s) 283, 619
Bsp13I TCCGGA 1 cut(s) 13
Bsp143I GATC 4 cut(s) 178, 414, 553, 748
BspACI CCGC 3 cut(s) 655, 683, 788
BspEI TCCGGA 1 cut(s) 13
BspLI GGNNCC 2 cut(s) 282, 301
BspPI GGATC 1 cut(s) 173
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 2 cut(s) 11, 776
BsrDI GCAATG 2 cut(s) 55, 674
BsrI ACTGG 1 cut(s) 117
BssECI CCNNGG 3 cut(s) 303, 383, 509
BssMI GATC 4 cut(s) 178, 414, 553, 748
BssT1I CCWWGG 1 cut(s) 509
Bst2UI CCWGG 3 cut(s) 135, 194, 384
Bst4CI ACNGT 1 cut(s) 130
BstBAI YACGTR 1 cut(s) 443
BstC8I GCNNGC 5 cut(s) 340, 536, 655, 786, 793
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 619
BstH2I RGCGCY 1 cut(s) 717
BstHHI GCGC 2 cut(s) 534, 716
BstKTI GATC 4 cut(s) 181, 417, 556, 751
BstMAI GTCTC 2 cut(s) 11, 776
BstMBI GATC 4 cut(s) 178, 414, 553, 748
BstMWI GCNNNNNNNGC 1 cut(s) 608
BstNI CCWGG 3 cut(s) 135, 194, 384
BstNSI RCATGY 4 cut(s) 94, 538, 615, 788
BstSCI CCNGG 3 cut(s) 133, 192, 382
BstSLI GKGCMC 1 cut(s) 619
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 1 cut(s) 581
BstXI CCANNNNNNTGG 1 cut(s) 229
BtgI CCRYGG 1 cut(s) 303
BtsCI GGATG 1 cut(s) 619
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 5 cut(s) 340, 536, 655, 786, 793
CfoI GCGC 2 cut(s) 534, 716
Cfr13I GGNCC 1 cut(s) 400
CsiI ACCWGGT 1 cut(s) 192
Csp6I GTAC 1 cut(s) 94
CviAII CATG 6 cut(s) 22, 91, 535, 612, 752, 785
CviQI GTAC 1 cut(s) 94
DpnI GATC 4 cut(s) 180, 416, 555, 750
DpnII GATC 4 cut(s) 178, 414, 553, 748
Eco130I CCWWGG 1 cut(s) 509
Eco24I GRGCYC 1 cut(s) 283
Eco31I GGTCTC 2 cut(s) 11, 776
Eco32I GATATC 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 400
Eco72I CACGTG 1 cut(s) 443
EcoRII CCWGG 3 cut(s) 133, 192, 382
EcoRV GATATC 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 509
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 509
FaeI CATG 6 cut(s) 25, 94, 538, 615, 755, 788
FatI CATG 6 cut(s) 21, 90, 534, 611, 751, 784
FauI CCCGC 1 cut(s) 662
FauNDI CATATG 1 cut(s) 260
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 2 cut(s) 371, 789
FokI GGATG 1 cut(s) 606
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 2 cut(s) 371, 789
FspBI CTAG 2 cut(s) 350, 605
GlaI GCGC 2 cut(s) 533, 715
GluI GCNGC 2 cut(s) 371, 789
GsuI CTGGAG 4 cut(s) 156, 267, 525, 705
HaeII RGCGCY 1 cut(s) 717
HapII CCGG 2 cut(s) 14, 711
HhaI GCGC 2 cut(s) 534, 716
Hin1II CATG 6 cut(s) 25, 94, 538, 615, 755, 788
Hin6I GCGC 2 cut(s) 532, 714
HinP1I GCGC 2 cut(s) 532, 714
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 4 cut(s) 242, 463, 644, 742
HpaII CCGG 2 cut(s) 14, 711
HphI GGTGA 3 cut(s) 182, 244, 251
Hpy166II GTNNAC 3 cut(s) 266, 400, 617
Hpy188I TCNGA 1 cut(s) 107
Hpy188III TCNNGA 8 cut(s) 14, 34, 284, 542, 564, 605, 722, 746
Hpy8I GTNNAC 3 cut(s) 266, 400, 617
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 2 cut(s) 268, 442
HpyCH4V TGCA 5 cut(s) 53, 395, 597, 617, 784
HpyF10VI GCNNNNNNNGC 1 cut(s) 608
HpySE526I ACGT 2 cut(s) 268, 442
Hsp92II CATG 6 cut(s) 25, 94, 538, 615, 755, 788
HspAI GCGC 2 cut(s) 532, 714
Kpn2I TCCGGA 1 cut(s) 13
Ksp22I TGATCA 1 cut(s) 748
Kzo9I GATC 4 cut(s) 178, 414, 553, 748
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 1 cut(s) 382
MabI ACCWGGT 1 cut(s) 192
MaeI CTAG 2 cut(s) 350, 605
MaeII ACGT 2 cut(s) 268, 442
MalI GATC 4 cut(s) 180, 416, 555, 750
MboI GATC 4 cut(s) 178, 414, 553, 748
MboII GAAGA 2 cut(s) 458, 581
MfeI CAATTG 2 cut(s) 321, 549
MhlI GDGCHC 2 cut(s) 283, 619
MluCI AATT 6 cut(s) 166, 321, 450, 469, 549, 691
MlyI GAGTC 1 cut(s) 653
MnlI CCTC 5 cut(s) 101, 171, 192, 549, 690
MroI TCCGGA 1 cut(s) 13
MroXI GAANNNNTTC 2 cut(s) 9, 450
MseI TTAA 2 cut(s) 690, 696
MslI CAYNNNNRTG 1 cut(s) 259
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 2 cut(s) 14, 711
MspR9I CCNGG 3 cut(s) 135, 194, 384
MunI CAATTG 2 cut(s) 321, 549
Mva1269I GAATGC 1 cut(s) 69
MvaI CCWGG 3 cut(s) 135, 194, 384
MwoI GCNNNNNNNGC 1 cut(s) 608
NdeI CATATG 1 cut(s) 260
NdeII GATC 4 cut(s) 178, 414, 553, 748
NlaIII CATG 6 cut(s) 25, 94, 538, 615, 755, 788
NlaIV GGNNCC 2 cut(s) 282, 301
NspI RCATGY 4 cut(s) 94, 538, 615, 788
PaeI GCATGC 2 cut(s) 538, 788
PciI ACATGT 1 cut(s) 90
PctI GAATGC 1 cut(s) 69
PdmI GAANNNNTTC 2 cut(s) 9, 450
PfeI GAWTC 3 cut(s) 242, 463, 742
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 2 cut(s) 372, 790
PleI GAGTC 1 cut(s) 652
PmaCI CACGTG 1 cut(s) 443
PmlI CACGTG 1 cut(s) 443
PpsI GAGTC 1 cut(s) 652
Ppu21I YACGTR 1 cut(s) 443
PscI ACATGT 1 cut(s) 90
Psp6I CCWGG 3 cut(s) 133, 192, 382
PspCI CACGTG 1 cut(s) 443
PspGI CCWGG 3 cut(s) 133, 192, 382
PspN4I GGNNCC 2 cut(s) 282, 301
PspPI GGNCC 1 cut(s) 400
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 259
SaqAI TTAA 2 cut(s) 690, 696
SatI GCNGC 2 cut(s) 371, 789
Sau3AI GATC 4 cut(s) 178, 414, 553, 748
Sau96I GGNCC 1 cut(s) 400
SchI GAGTC 1 cut(s) 653
ScrFI CCNGG 3 cut(s) 135, 194, 384
SduI GDGCHC 2 cut(s) 283, 619
SexAI ACCWGGT 1 cut(s) 192
SinI GGWCC 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 259
SphI GCATGC 2 cut(s) 538, 788
Sse9I AATT 6 cut(s) 166, 321, 450, 469, 549, 691
SsiI CCGC 3 cut(s) 655, 683, 788
SspI AATATT 1 cut(s) 294
SspMI CTAG 2 cut(s) 350, 605
StyD4I CCNGG 3 cut(s) 133, 192, 382
StyI CCWWGG 1 cut(s) 509
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 2 cut(s) 271, 445
TaqI TCGA 1 cut(s) 177
TasI AATT 6 cut(s) 166, 321, 450, 469, 549, 691
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 3 cut(s) 242, 463, 742
Tru1I TTAA 2 cut(s) 690, 696
Tru9I TTAA 2 cut(s) 690, 696
TscAI CASTG 1 cut(s) 402
TseI GCWGC 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 476
TspGWI ACGGA 2 cut(s) 320, 629
TspRI CASTG 1 cut(s) 402
VneI GTGCAC 1 cut(s) 615
VpaK11BI GGWCC 1 cut(s) 400
XapI RAATTY 2 cut(s) 166, 450
XbaI TCTAGA 1 cut(s) 604
XceI RCATGY 4 cut(s) 94, 538, 615, 788
XmnI GAANNNNTTC 2 cut(s) 9, 450
XspI CTAG 2 cut(s) 350, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.