Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0009149

Physical Location & Seq

Genomic Coordinates Reverse (-)

8682132 .. 8688129

Gene Structure 5998 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG094600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGCAGAGAGCTTCCATTCTCTGTTGTTCAAATCCACTCCTTCCCATCCCCACTTTCCCACCCACCACTCATAAACTCGCTGCCGCTTCCCTTCAAACTCGGCACACTCGTCTTGACTCGGTTTCCTCACCTCTCCGACTTCCCCTGAATTACCGCCTTGCCCTCCCTCCCCTACACAGCACTGCCACTGAAGAGATTGTTGAGACTGCTGAGGCGGTTAGAGGATTCGTTAGTGTTGGTTATGTTGCCAATGTCCATGGGCTTCAAGGAGAGATTCGTGTGAAACCTAGCACTGACTTTCCTGAATTGCGGTTTGCTGAGCCTGGAAGAAGATGGCTGCGGCAACAAATATCAGGCCAAGAAAGAGTTGAAGAAGTTGAACTGGTGGAGGGAAGAGGACACCCTGGCCAGAAGAGTTGGATACTTAGATTGAGAGGAATTGACGATGTGGATCAGGCTAGGCAGCTTATTGGTTCAACTTTACTTGTGAGGGAAGAAGATAGGCCAGAGTTGGAAGAAGGTGAAATCTATAGTCGTGATCTCCTCGGAATGAGAGTTATTCTCAAGGAAACAGGTAAACCTGTGGGAAATGTTGTTAATGTTTTCAACAGCGGAGCAAGCGATCTTCTACAGGTCATGCTTGATTCATCGCTTGATATTCTTGACTCAACTGGCAAGCCAAAGCCAGCAGGAACAACATTATCTGGTCACCTTGTGTTGATACCGTTTGTCGAAGAAATTGTTCCAGATGTTGATATAAAGAATAAAGAAATGCAGATTACACCTCCAAAGGGACTCCTTGAACTAAATTTACGCTTTGATGAGAGATCGAAGAAAGAAAGGCGCCAGATTGAATGGAAAGAGAGAAAAAAGTCCCAGAAGCGCCTCATAGCAGCAAAAAAGAAGCTGTGTGAAATGGATCAAAAACATGTGTTTGATGGGTTAAGATTTGGAGAGAAAGCACAACGGAGCTTACTTGCTGATCAGATTGTTGGTGTTAATTCACTGTTACTTCAACAAGCTCTAGAAAATGTTGAGATACGTTCTAAAAGGTTGAATGTGACTGAGATGGTCAGCGCGCTTAAAGCAGAAGAAACAAGTAGTACTCTGGAAATATCAGAGAAATGTCTAATTTCCTCTGCAAGTGAAGATAAGCTGAGTACACATTCCCATCTTCAAGAGAAGGGAGTAGATCTTTTATCTAAAGGCAAAGTTGCCATTGCTTTAGTCGTAAACAATTCTTACGACTCAGAGAACACTATGTCTTCCTTTGTTCAGACATTACTTCGTGACGATCAGAGACTTTTAAAGATGGAAGACCGTGCCTTGGTTCCTTTGATTTTGGTTTGCTCAGCTCAGAAAATTGAATCTTTAAGGATGCATTTCTCAGAAAATGATTACTTCGGCTTTGACTCGGGAAAGGTTTGTAGTTACAGCATCATCCCATCAAGAGTCTCTCTTTATAATTACGATATTGCACTATGGCATAGCATGGTGCTAAAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 ko01130 map00520 map01100 map01130

Pfam Domains

Protein Families

PRC RimM UDPGP

Protein Analysis

502

Amino Acids

56.4

Weight (kDa)

6.86

Isoelectric Point (pI)

50.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RimM	PF01782	79 - 166	4.6e-20	RimM N-terminal domain
PRC	PF05239	173 - 267	7.4e-09	PRC-barrel domain
PRC_RimM	PF24986	180 - 266	2.3e-12	RimM PRC barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009149)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46420
fragaria_vesca	FvH4_3g06410 FvH4_3g06410 FvH4_3g06410 FvH4_3g06410 FvH4_3g06410 FvH4_3g06410 FvH4_3g06410
malus_domestica	MD05G1291800.v1.1
prunus_persica	Prupe.4G073700_v2.0.a1
pyrus_communis	pycom05g26830
rosa_chinensis	RchiOBHm_Chr5g0012901
rosa_laevigata	RLG00000031967
rosa_multiflora	Rmu_co8505517.1_g000001 Rmu_sc0010496.1_g000008
rosa_roxburghii	Rroxscaffold_1G00063680
rosa_rugosa	Rorug05G0005100
rosa_samantha	Rh5AG099300 Rh5BG095900 Rh5CG108200 Rh5DG094600
rosa_wichuraiana	Rw5G008670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1464
AccB1I	GGYRCC	1 cut(s)	843
AccII	CGCG	1 cut(s)	1079
AciI	CCGC	6 cut(s)	84, 154, 215, 310, 340, 612
AclWI	GGATC	2 cut(s)	459, 927
AcoI	YGGCCR	1 cut(s)	406
AcsI	RAATTY	1 cut(s)	808
AcuI	CTGAAG	1 cut(s)	210
AcyI	GRCGYC	1 cut(s)	844
AdeI	CACNNNGTG	1 cut(s)	715
AfaI	GTAC	2 cut(s)	1105, 1162
AfiI	CCNNNNNNNGG	2 cut(s)	791, 1327
AflIII	ACRYGT	1 cut(s)	928
AjnI	CCWGG	2 cut(s)	322, 403
AluBI	AGCT	7 cut(s)	11, 466, 907, 972, 1022, 1156, 1355
AluI	AGCT	7 cut(s)	11, 466, 907, 972, 1022, 1156, 1355
Alw26I	GTCTC	3 cut(s)	197, 1294, 1459
AlwI	GGATC	2 cut(s)	459, 927
Ama87I	CYCGRG	1 cut(s)	1414
AoxI	GGCC	3 cut(s)	355, 406, 503
ApeKI	GCWGC	4 cut(s)	80, 337, 463, 893
ApoI	RAATTY	1 cut(s)	808
Asp700I	GAANNNNTTC	1 cut(s)	741
AspLEI	GCGC	4 cut(s)	846, 885, 1079, 1081
AsuHPI	GGTGA	3 cut(s)	120, 533, 701
AvaI	CYCGRG	1 cut(s)	1414
BalI	TGGCCA	1 cut(s)	408
BanI	GGYRCC	1 cut(s)	843
BbsI	GAAGAC	2 cut(s)	1257, 1323
BbvCI	CCTCAGC	1 cut(s)	210
BbvI	GCAGC	4 cut(s)	67, 324, 475, 905
BccI	CCATC	7 cut(s)	53, 327, 932, 1063, 1179, 1306, 1453
BciT130I	CCWGG	2 cut(s)	324, 405
BciVI	GTATCC	1 cut(s)	414
BclI	TGATCA	1 cut(s)	982
BcoDI	GTCTC	3 cut(s)	197, 1294, 1459
BfaI	CTAG	3 cut(s)	288, 459, 1025
BfmI	CTRYAG	2 cut(s)	529, 629
BfoI	RGCGCY	2 cut(s)	847, 886
BfuI	GTATCC	1 cut(s)	414
BglII	AGATCT	1 cut(s)	1192
BisI	GCNGC	6 cut(s)	81, 84, 338, 341, 464, 894
BlpI	GCTNAGC	2 cut(s)	318, 1351
BlsI	GCNGC	6 cut(s)	82, 85, 339, 342, 465, 895
BmcAI	AGTACT	1 cut(s)	1105
Bme1390I	CCNGG	2 cut(s)	324, 405
BmeT110I	CYCGRG	1 cut(s)	1414
BmiI	GGNNCC	2 cut(s)	845, 1332
BmrFI	CCNGG	2 cut(s)	324, 405
BmsI	GCATC	2 cut(s)	1368, 1446
BpiI	GAAGAC	2 cut(s)	1257, 1323
BplI	GAGNNNNNCTC	2 cut(s)	546, 578
Bpu10I	CCTNAGC	1 cut(s)	210
Bpu1102I	GCTNAGC	2 cut(s)	318, 1351
BpuEI	CTTGAG	1 cut(s)	548
BsaBI	GATNNNNATC	1 cut(s)	450
BsaHI	GRCGYC	1 cut(s)	844
BsaJI	CCNNGG	4 cut(s)	256, 403, 544, 1326
Bsc4I	CCNNNNNNNGG	2 cut(s)	791, 1327
Bse1I	ACTGG	2 cut(s)	387, 676
Bse3DI	GCAATG	1 cut(s)	1218
Bse8I	GATNNNNATC	1 cut(s)	450
BseBI	CCWGG	2 cut(s)	324, 405
BseDI	CCNNGG	4 cut(s)	256, 403, 544, 1326
BseGI	GGATG	3 cut(s)	45, 1383, 1440
BseJI	GATNNNNATC	1 cut(s)	450
BseLI	CCNNNNNNNGG	2 cut(s)	791, 1327
BseMI	GCAATG	1 cut(s)	1218
BseMII	CTCAG	8 cut(s)	201, 309, 1056, 1148, 1263, 1365, 1370, 1401
BseNI	ACTGG	2 cut(s)	387, 676
BsePI	GCGCGC	1 cut(s)	1077
BseRI	GAGGAG	1 cut(s)	533
BseXI	GCAGC	4 cut(s)	67, 324, 475, 905
Bsh1236I	CGCG	1 cut(s)	1079
BshFI	GGCC	3 cut(s)	357, 408, 505
BshNI	GGYRCC	1 cut(s)	843
BsiHKCI	CYCGRG	1 cut(s)	1414
BslFI	GGGAC	2 cut(s)	807, 859
BslI	CCNNNNNNNGG	2 cut(s)	791, 1327
BsmAI	GTCTC	3 cut(s)	197, 1294, 1459
BsmFI	GGGAC	2 cut(s)	807, 859
BsnI	GGCC	3 cut(s)	357, 408, 505
BsoBI	CYCGRG	1 cut(s)	1414
Bsp143I	GATC	8 cut(s)	451, 538, 622, 827, 919, 982, 1192, 1294
Bsp1720I	GCTNAGC	2 cut(s)	318, 1351
Bsp19I	CCATGG	1 cut(s)	256
BspACI	CCGC	6 cut(s)	84, 154, 215, 310, 340, 612
BspANI	GGCC	3 cut(s)	357, 408, 505
BspCNI	CTCAG	8 cut(s)	202, 310, 1057, 1149, 1262, 1364, 1369, 1400
BspFNI	CGCG	1 cut(s)	1079
BspLI	GGNNCC	2 cut(s)	845, 1332
BspPI	GGATC	2 cut(s)	459, 927
BspT107I	GGYRCC	1 cut(s)	843
BsrDI	GCAATG	1 cut(s)	1218
BsrI	ACTGG	2 cut(s)	387, 676
BssECI	CCNNGG	4 cut(s)	256, 403, 544, 1326
BssHII	GCGCGC	1 cut(s)	1077
BssMI	GATC	8 cut(s)	451, 538, 622, 827, 919, 982, 1192, 1294
BssNI	GRCGYC	1 cut(s)	844
BssT1I	CCWWGG	2 cut(s)	256, 1326
Bst2UI	CCWGG	2 cut(s)	324, 405
Bst4CI	ACNGT	3 cut(s)	726, 1008, 1322
Bst6I	CTCTTC	3 cut(s)	186, 388, 407
BstACI	GRCGYC	1 cut(s)	844
BstC8I	GCNNGC	4 cut(s)	619, 677, 687, 1079
BstDEI	CTNAG	9 cut(s)	210, 318, 425, 1065, 1157, 1249, 1351, 1356, 1387
BstDSI	CCRYGG	1 cut(s)	256
BstEII	GGTNACC	1 cut(s)	707
BstF5I	GGATG	3 cut(s)	45, 1383, 1440
BstFNI	CGCG	1 cut(s)	1079
BstH2I	RGCGCY	2 cut(s)	847, 886
BstHHI	GCGC	4 cut(s)	846, 885, 1079, 1081
BstKTI	GATC	8 cut(s)	454, 541, 625, 830, 922, 985, 1195, 1297
BstMAI	GTCTC	3 cut(s)	197, 1294, 1459
BstMBI	GATC	8 cut(s)	451, 538, 622, 827, 919, 982, 1192, 1294
BstMWI	GCNNNNNNNGC	2 cut(s)	618, 1085
BstNI	CCWGG	2 cut(s)	324, 405
BstNSI	RCATGY	1 cut(s)	932
BstPI	GGTNACC	1 cut(s)	707
BstSCI	CCNGG	2 cut(s)	322, 403
BstSFI	CTRYAG	2 cut(s)	529, 629
BstUI	CGCG	1 cut(s)	1079
BstV1I	GCAGC	4 cut(s)	67, 324, 475, 905
BstV2I	GAAGAC	2 cut(s)	1257, 1323
BstX2I	RGATCY	1 cut(s)	1192
BstYI	RGATCY	1 cut(s)	1192
BsuI	GTATCC	1 cut(s)	414
BsuRI	GGCC	3 cut(s)	357, 408, 505
BtgI	CCRYGG	1 cut(s)	256
BtgZI	GCGATG	1 cut(s)	633
BtsCI	GGATG	3 cut(s)	45, 1383, 1440
BtsI	GCAGTG	1 cut(s)	180
BtsIMutI	CAGTG	4 cut(s)	180, 186, 291, 1004
Cac8I	GCNNGC	4 cut(s)	619, 677, 687, 1079
CfoI	GCGC	4 cut(s)	846, 885, 1079, 1081
Csp6I	GTAC	2 cut(s)	1104, 1161
CviAII	CATG	4 cut(s)	257, 637, 929, 1492
CviQI	GTAC	2 cut(s)	1104, 1161
DdeI	CTNAG	9 cut(s)	210, 318, 425, 1065, 1157, 1249, 1351, 1356, 1387
DinI	GGCGCC	1 cut(s)	845
DpnI	GATC	8 cut(s)	453, 540, 624, 829, 921, 984, 1194, 1296
DpnII	GATC	8 cut(s)	451, 538, 622, 827, 919, 982, 1192, 1294
DraI	TTTAAA	1 cut(s)	1308
DraIII	CACNNNGTG	1 cut(s)	715
EaeI	YGGCCR	1 cut(s)	406
Eam1104I	CTCTTC	3 cut(s)	186, 388, 407
EarI	CTCTTC	3 cut(s)	186, 388, 407
Eco130I	CCWWGG	2 cut(s)	256, 1326
Eco57I	CTGAAG	1 cut(s)	210
Eco88I	CYCGRG	1 cut(s)	1414
Eco91I	GGTNACC	1 cut(s)	707
EcoO65I	GGTNACC	1 cut(s)	707
EcoRII	CCWGG	2 cut(s)	322, 403
EcoT14I	CCWWGG	2 cut(s)	256, 1326
EcoT22I	ATGCAT	1 cut(s)	1383
EgeI	GGCGCC	1 cut(s)	845
EheI	GGCGCC	1 cut(s)	845
ErhI	CCWWGG	2 cut(s)	256, 1326
FaeI	CATG	4 cut(s)	260, 640, 932, 1495
FaqI	GGGAC	2 cut(s)	807, 859
FatI	CATG	4 cut(s)	256, 636, 928, 1491
FbaI	TGATCA	1 cut(s)	982
Fnu4HI	GCNGC	6 cut(s)	81, 84, 338, 341, 464, 894
FokI	GGATG	3 cut(s)	32, 1390, 1427
Fsp4HI	GCNGC	6 cut(s)	81, 84, 338, 341, 464, 894
FspBI	CTAG	3 cut(s)	288, 459, 1025
GlaI	GCGC	4 cut(s)	845, 884, 1078, 1080
GluI	GCNGC	6 cut(s)	81, 84, 338, 341, 464, 894
HaeII	RGCGCY	2 cut(s)	847, 886
HaeIII	GGCC	3 cut(s)	357, 408, 505
HhaI	GCGC	4 cut(s)	846, 885, 1079, 1081
Hin1I	GRCGYC	1 cut(s)	844
Hin1II	CATG	4 cut(s)	260, 640, 932, 1495
Hin6I	GCGC	4 cut(s)	844, 883, 1077, 1079
HinP1I	GCGC	4 cut(s)	844, 883, 1077, 1079
HphI	GGTGA	3 cut(s)	120, 533, 701
Hpy166II	GTNNAC	3 cut(s)	578, 1163, 1234
Hpy188I	TCNGA	9 cut(s)	137, 548, 987, 1120, 1252, 1278, 1299, 1359, 1390
Hpy8I	GTNNAC	3 cut(s)	578, 1163, 1234
HpyAV	CCTTC	4 cut(s)	50, 101, 512, 1177
HpyCH4III	ACNGT	3 cut(s)	726, 1008, 1322
HpyCH4IV	ACGT	1 cut(s)	1042
HpyCH4V	TGCA	5 cut(s)	4, 775, 1142, 1381, 1478
HpyF10VI	GCNNNNNNNGC	2 cut(s)	618, 1085
HpyF3I	CTNAG	9 cut(s)	210, 318, 425, 1065, 1157, 1249, 1351, 1356, 1387
HpySE526I	ACGT	1 cut(s)	1042
Hsp92I	GRCGYC	1 cut(s)	844
Hsp92II	CATG	4 cut(s)	260, 640, 932, 1495
HspAI	GCGC	4 cut(s)	844, 883, 1077, 1079
KasI	GGCGCC	1 cut(s)	843
Ksp22I	TGATCA	1 cut(s)	982
Kzo9I	GATC	8 cut(s)	451, 538, 622, 827, 919, 982, 1192, 1294
LmnI	GCTCC	2 cut(s)	614, 969
Lsp1109I	GCAGC	4 cut(s)	67, 324, 475, 905
LweI	GCATC	2 cut(s)	1368, 1446
MaeI	CTAG	3 cut(s)	288, 459, 1025
MaeII	ACGT	1 cut(s)	1042
MaeIII	GTNAC	5 cut(s)	707, 1008, 1060, 1289, 1430
MalI	GATC	8 cut(s)	453, 540, 624, 829, 921, 984, 1194, 1296
MboI	GATC	8 cut(s)	451, 538, 622, 827, 919, 982, 1192, 1294
MflI	RGATCY	1 cut(s)	1192
MlsI	TGGCCA	1 cut(s)	408
MluNI	TGGCCA	1 cut(s)	408
Mly113I	GGCGCC	1 cut(s)	844
MlyI	GAGTC	6 cut(s)	110, 659, 789, 1241, 1406, 1461
MmeI	TCCRAC	3 cut(s)	160, 398, 492
Mox20I	TGGCCA	1 cut(s)	408
Mph1103I	ATGCAT	1 cut(s)	1383
MroXI	GAANNNNTTC	1 cut(s)	741
MscI	TGGCCA	1 cut(s)	408
MseI	TTAA	6 cut(s)	597, 944, 999, 1083, 1307, 1373
Msp20I	TGGCCA	1 cut(s)	408
MspA1I	CMGCKG	1 cut(s)	612
MspR9I	CCNGG	2 cut(s)	324, 405
MvaI	CCWGG	2 cut(s)	324, 405
MvnI	CGCG	1 cut(s)	1079
MwoI	GCNNNNNNNGC	2 cut(s)	618, 1085
NarI	GGCGCC	1 cut(s)	844
NcoI	CCATGG	1 cut(s)	256
NdeII	GATC	8 cut(s)	451, 538, 622, 827, 919, 982, 1192, 1294
NlaIII	CATG	4 cut(s)	260, 640, 932, 1495
NlaIV	GGNNCC	2 cut(s)	845, 1332
NmeAIII	GCCGAG	1 cut(s)	79
NmuCI	GTSAC	3 cut(s)	707, 1060, 1289
NsiI	ATGCAT	1 cut(s)	1383
NspI	RCATGY	1 cut(s)	932
PauI	GCGCGC	1 cut(s)	1077
PciI	ACATGT	1 cut(s)	928
PcsI	WCGNNNNNNNCGW	1 cut(s)	106
PdmI	GAANNNNTTC	1 cut(s)	741
PfeI	GAWTC	4 cut(s)	225, 274, 644, 1367
PkrI	GCNGC	6 cut(s)	82, 85, 339, 342, 465, 895
PleI	GAGTC	6 cut(s)	110, 659, 789, 1241, 1406, 1460
PluTI	GGCGCC	1 cut(s)	847
PpsI	GAGTC	6 cut(s)	110, 659, 789, 1241, 1406, 1460
PscI	ACATGT	1 cut(s)	928
PsiI	TTATAA	1 cut(s)	1464
Psp6I	CCWGG	2 cut(s)	322, 403
PspEI	GGTNACC	1 cut(s)	707
PspGI	CCWGG	2 cut(s)	322, 403
PspN4I	GGNNCC	2 cut(s)	845, 1332
PsrI	GAACNNNNNNTAC	2 cut(s)	795, 827
PsuI	RGATCY	1 cut(s)	1192
PteI	GCGCGC	1 cut(s)	1077
RsaI	GTAC	2 cut(s)	1105, 1162
RsaNI	GTAC	2 cut(s)	1104, 1161
SaqAI	TTAA	6 cut(s)	597, 944, 999, 1083, 1307, 1373
SatI	GCNGC	6 cut(s)	81, 84, 338, 341, 464, 894
Sau3AI	GATC	8 cut(s)	451, 538, 622, 827, 919, 982, 1192, 1294
ScaI	AGTACT	1 cut(s)	1105
SchI	GAGTC	6 cut(s)	110, 659, 789, 1241, 1406, 1461
ScrFI	CCNGG	2 cut(s)	324, 405
SfaNI	GCATC	2 cut(s)	1368, 1446
SfcI	CTRYAG	2 cut(s)	529, 629
SfoI	GGCGCC	1 cut(s)	845
SmlI	CTYRAG	1 cut(s)	563
SmoI	CTYRAG	1 cut(s)	563
SsiI	CCGC	6 cut(s)	84, 154, 215, 310, 340, 612
SspDI	GGCGCC	1 cut(s)	843
SspMI	CTAG	3 cut(s)	288, 459, 1025
StyD4I	CCNGG	2 cut(s)	322, 403
StyI	CCWWGG	2 cut(s)	256, 1326
TaaI	ACNGT	3 cut(s)	726, 1008, 1322
TaiI	ACGT	1 cut(s)	1045
TaqI	TCGA	2 cut(s)	732, 830
TatI	WGTACW	2 cut(s)	1103, 1160
TauI	GCSGC	2 cut(s)	86, 343
TfiI	GAWTC	4 cut(s)	225, 274, 644, 1367
Tru1I	TTAA	6 cut(s)	597, 944, 999, 1083, 1307, 1373
Tru9I	TTAA	6 cut(s)	597, 944, 999, 1083, 1307, 1373
TscAI	CASTG	4 cut(s)	187, 193, 298, 1011
TseFI	GTSAC	3 cut(s)	707, 1060, 1289
TseI	GCWGC	4 cut(s)	80, 337, 463, 893
Tsp45I	GTSAC	3 cut(s)	707, 1060, 1289
TspDTI	ATGAA	1 cut(s)	636
TspGWI	ACGGA	1 cut(s)	982
TspRI	CASTG	4 cut(s)	187, 193, 298, 1011
XapI	RAATTY	1 cut(s)	808
XbaI	TCTAGA	1 cut(s)	1024
XceI	RCATGY	1 cut(s)	932
XmnI	GAANNNNTTC	1 cut(s)	741
XspI	CTAG	3 cut(s)	288, 459, 1025
ZrmI	AGTACT	1 cut(s)	1105
Zsp2I	ATGCAT	1 cut(s)	1383

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.