Rorug05G0005100

RimM N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001229
Physical Location & Seq
Forward (+)
314215 .. 317895
3681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0005100.1

Sequence Viewer

Length: 888 bp
ATGGGGAATGTGAATGGGAGAGAAGATGGCGGCGGAAGCCCATCGGCGGCGGCGGCCGAGGAAGAGGCCGGTGGCGGGACCTCCCAGGAGGGTCATCACCCCCTTCCGCCTCCACTTGGCACCGGCGGAGGCCGTGGCGGAGGCGCGGTGGCTGAATTGATGGGTCAGTCACCTCCTCATAGCCCTAGGGCTACTCACTCCCCTTTGATGTTCACTCCCCAAGTCCCCGTTGTTCCGTTACAAAGACCTGATGAGATGCAGATCCCAAACCCCTCATGGATGCAGACTTCAGCATATGAAGACATCTCCTGTGAGCAAGGAATTCCAACCATGATTACGTGGGGCTATGGTGGGAAGGACGTAGCTGTGGAGGGATCATGGGATGATTGGAAGACAAGGTTGGCCTTACAGAGATCAGGGAAGGACTTCACTATTATGAAAGTACTGCCATCCGGTGTTTACCAGTATAGGTTTGTTGTTGACGGGCAGTGGAGGTGTGCTCCAGACTTGCCTTTGGCCCAAGATGATGCTGGAAATTCTTACAATCTTTTGGACTTGCAGGATTATGTTCCTGAAGACATTGGAAGCATTTCTGGTTTTGAACCTCCCCAGTCTCCTGACTCGAGTTATAGCAACATGCAACTTGGATCTGAAGATTTTGCGAAGGAGCCACCATTGGTACCTCCACATCTGCAGATGACGCTGCTCAATGCCCCAGCATCTTACATGGAGATGCCGCCTCCTTTATCAAGACCTCAACATGTTGTACTTAATCATCTATACATGCAGAGAGGGAAGAGTGGCCCATCTGTGGTCGCACTTGGCACAACAGAGAGGTTTATAGCAAAGTATGTGACAGTTGTTCTCTACAAGTCTTTGCAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 ko01130 map00520 map01100 map01130
Pfam Domains
Protein Families
PRC RimM UDPGP

Protein Analysis

295

Amino Acids

31.87

Weight (kDa)

4.96

Isoelectric Point (pI)

57.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 108 - 187 1.8e-31 Glycogen recognition site of AMP-activated protein kinase
AMPKBI PF04739 220 - 292 2.2e-25 5'-AMP-activated protein kinase beta subunit, interaction domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 679
AccB1I GGYRCC 2 cut(s) 119, 679
AccII CGCG 1 cut(s) 146
AclWI GGATC 3 cut(s) 256, 382, 655
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 2 cut(s) 321, 535
AcuI CTGAAG 3 cut(s) 273, 594, 672
AfaI GTAC 3 cut(s) 444, 681, 768
AfiI CCNNNNNNNGG 4 cut(s) 46, 75, 116, 811
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 1 cut(s) 602
AjnI CCWGG 1 cut(s) 84
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 1 cut(s) 365
AluI AGCT 1 cut(s) 365
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 1 cut(s) 618
AlwI GGATC 3 cut(s) 256, 382, 655
Ama87I CYCGRG 1 cut(s) 622
AoxI GGCC 6 cut(s) 54, 66, 130, 402, 516, 802
ApeKI GCWGC 1 cut(s) 703
ApoI RAATTY 2 cut(s) 321, 535
Asp700I GAANNNNTTC 2 cut(s) 425, 589
Asp718I GGTACC 1 cut(s) 679
AspA2I CCTAGG 1 cut(s) 185
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 3 cut(s) 78, 517, 803
AsuHPI GGTGA 2 cut(s) 89, 162
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 1 cut(s) 78
AvrII CCTAGG 1 cut(s) 185
BanI GGYRCC 2 cut(s) 119, 679
BbsI GAAGAC 3 cut(s) 306, 398, 582
Bbv12I GWGCWC 1 cut(s) 502
BbvI GCAGC 1 cut(s) 690
BccI CCATC 5 cut(s) 20, 49, 154, 457, 814
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 618
BfaI CTAG 1 cut(s) 186
BfmI CTRYAG 1 cut(s) 692
BisI GCNGC 6 cut(s) 31, 48, 51, 54, 704, 737
BlnI CCTAGG 1 cut(s) 185
BlsI GCNGC 6 cut(s) 32, 49, 52, 55, 705, 738
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 1 cut(s) 78
BmeT110I CYCGRG 1 cut(s) 622
BmgT120I GGNCC 3 cut(s) 78, 517, 803
BmiI GGNNCC 4 cut(s) 79, 121, 669, 681
BmrFI CCNGG 1 cut(s) 86
BmrI ACTGGG 1 cut(s) 604
BmsI GCATC 5 cut(s) 246, 270, 517, 723, 728
BmuI ACTGGG 1 cut(s) 604
BpiI GAAGAC 3 cut(s) 306, 398, 582
BplI GAGNNNNNCTC 2 cut(s) 484, 516
BpmI CTGGAG 1 cut(s) 486
BsaAI YACGTR 1 cut(s) 339
BsaBI GATNNNNATC 1 cut(s) 260
BsaJI CCNNGG 4 cut(s) 57, 84, 133, 185
BsaWI WCCGGW 1 cut(s) 452
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 75, 116, 811
Bse118I RCCGGY 2 cut(s) 68, 122
Bse1I ACTGG 2 cut(s) 463, 610
Bse8I GATNNNNATC 1 cut(s) 260
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 4 cut(s) 57, 84, 133, 185
BseGI GGATG 3 cut(s) 285, 388, 449
BseJI GATNNNNATC 1 cut(s) 260
BseLI CCNNNNNNNGG 4 cut(s) 46, 75, 116, 811
BseNI ACTGG 2 cut(s) 463, 610
BseRI GAGGAG 1 cut(s) 165
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 690
BseYI CCCAGC 1 cut(s) 715
Bsh1236I CGCG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 6 cut(s) 56, 68, 132, 404, 518, 804
BshNI GGYRCC 2 cut(s) 119, 679
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 3 cut(s) 69, 123, 453
BslFI GGGAC 2 cut(s) 91, 209
BslI CCNNNNNNNGG 4 cut(s) 46, 75, 116, 811
BsmAI GTCTC 1 cut(s) 618
BsmFI GGGAC 2 cut(s) 91, 209
BsnI GGCC 6 cut(s) 56, 68, 132, 404, 518, 804
BsoBI CYCGRG 1 cut(s) 622
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 4 cut(s) 261, 374, 413, 647
BspANI GGCC 6 cut(s) 56, 68, 132, 404, 518, 804
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 4 cut(s) 79, 121, 669, 681
BspMAI CTGCAG 1 cut(s) 696
BspPI GGATC 3 cut(s) 256, 382, 655
BspT107I GGYRCC 2 cut(s) 119, 679
BsrFI RCCGGY 2 cut(s) 68, 122
BsrI ACTGG 2 cut(s) 463, 610
BssAI RCCGGY 2 cut(s) 68, 122
BssECI CCNNGG 4 cut(s) 57, 84, 133, 185
BssMI GATC 4 cut(s) 261, 374, 413, 647
BssT1I CCWWGG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 859
Bst6I CTCTTC 2 cut(s) 57, 791
BstBAI YACGTR 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 133
BstF5I GGATG 3 cut(s) 285, 388, 449
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 4 cut(s) 264, 377, 416, 650
BstMAI GTCTC 1 cut(s) 618
BstMBI GATC 4 cut(s) 261, 374, 413, 647
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 3 cut(s) 36, 53, 700
BstNI CCWGG 1 cut(s) 86
BstNSI RCATGY 3 cut(s) 640, 764, 787
BstSCI CCNGG 1 cut(s) 84
BstSFI CTRYAG 1 cut(s) 692
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 690
BstV2I GAAGAC 3 cut(s) 306, 398, 582
BstX2I RGATCY 2 cut(s) 261, 647
BstYI RGATCY 2 cut(s) 261, 647
BstZI CGGCCG 1 cut(s) 54
BsuRI GGCC 6 cut(s) 56, 68, 132, 404, 518, 804
BtgI CCRYGG 1 cut(s) 133
BtsCI GGATG 3 cut(s) 285, 388, 449
BtsI GCAGTG 1 cut(s) 494
BtsIMutI CAGTG 1 cut(s) 494
CfoI GCGC 1 cut(s) 146
Cfr10I RCCGGY 2 cut(s) 68, 122
Cfr13I GGNCC 3 cut(s) 78, 517, 803
CseI GACGC 1 cut(s) 709
Csp6I GTAC 3 cut(s) 443, 680, 767
CviAII CATG 7 cut(s) 276, 331, 378, 637, 727, 761, 784
CviQI GTAC 3 cut(s) 443, 680, 767
DpnI GATC 4 cut(s) 263, 376, 415, 649
DpnII GATC 4 cut(s) 261, 374, 413, 647
EaeI YGGCCR 1 cut(s) 54
EagI CGGCCG 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 57, 791
EarI CTCTTC 2 cut(s) 57, 791
EciI GGCGGA 4 cut(s) 48, 96, 141, 153
EclXI CGGCCG 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 78
Eco52I CGGCCG 1 cut(s) 54
Eco57I CTGAAG 3 cut(s) 273, 594, 672
Eco88I CYCGRG 1 cut(s) 622
EcoO109I RGGNCCY 1 cut(s) 78
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
FaeI CATG 7 cut(s) 279, 334, 381, 640, 730, 764, 787
FaqI GGGAC 2 cut(s) 91, 209
FatI CATG 7 cut(s) 275, 330, 377, 636, 726, 760, 783
FauI CCCGC 1 cut(s) 68
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 6 cut(s) 31, 48, 51, 54, 704, 737
FokI GGATG 3 cut(s) 292, 395, 436
Fsp4HI GCNGC 6 cut(s) 31, 48, 51, 54, 704, 737
FspBI CTAG 1 cut(s) 186
GlaI GCGC 1 cut(s) 145
GluI GCNGC 6 cut(s) 31, 48, 51, 54, 704, 737
GsaI CCCAGC 1 cut(s) 719
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 6 cut(s) 56, 68, 132, 404, 518, 804
HapII CCGG 3 cut(s) 69, 123, 453
HgaI GACGC 1 cut(s) 709
HhaI GCGC 1 cut(s) 146
Hin1II CATG 7 cut(s) 279, 334, 381, 640, 730, 764, 787
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HinfI GANTC 1 cut(s) 620
HpaII CCGG 3 cut(s) 69, 123, 453
HphI GGTGA 2 cut(s) 89, 162
Hpy166II GTNNAC 3 cut(s) 213, 460, 481
Hpy188I TCNGA 1 cut(s) 652
Hpy188III TCNNGA 4 cut(s) 503, 572, 617, 750
Hpy8I GTNNAC 3 cut(s) 213, 460, 481
HpyAV CCTTC 4 cut(s) 113, 349, 415, 658
HpyCH4III ACNGT 1 cut(s) 859
HpyCH4IV ACGT 2 cut(s) 338, 360
HpyCH4V TGCA 7 cut(s) 259, 283, 559, 640, 694, 787, 880
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 53, 700
HpySE526I ACGT 2 cut(s) 338, 360
Hsp92II CATG 7 cut(s) 279, 334, 381, 640, 730, 764, 787
HspAI GCGC 1 cut(s) 144
KpnI GGTACC 1 cut(s) 683
Kzo9I GATC 4 cut(s) 261, 374, 413, 647
LmnI GCTCC 2 cut(s) 505, 667
Lsp1109I GCAGC 1 cut(s) 690
LweI GCATC 5 cut(s) 246, 270, 517, 723, 728
MaeI CTAG 1 cut(s) 186
MaeII ACGT 2 cut(s) 338, 360
MaeIII GTNAC 3 cut(s) 168, 237, 853
MalI GATC 4 cut(s) 263, 376, 415, 649
MboI GATC 4 cut(s) 261, 374, 413, 647
MboII GAAGA 7 cut(s) 35, 74, 311, 403, 587, 665, 808
MflI RGATCY 2 cut(s) 261, 647
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 3 cut(s) 155, 321, 535
MlyI GAGTC 1 cut(s) 614
MmeI TCCRAC 1 cut(s) 350
MroXI GAANNNNTTC 2 cut(s) 425, 589
MseI TTAA 1 cut(s) 771
MslI CAYNNNNRTG 2 cut(s) 434, 731
MspI CCGG 3 cut(s) 69, 123, 453
MspR9I CCNGG 1 cut(s) 86
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 3 cut(s) 36, 53, 700
NdeI CATATG 1 cut(s) 295
NdeII GATC 4 cut(s) 261, 374, 413, 647
NlaIII CATG 7 cut(s) 279, 334, 381, 640, 730, 764, 787
NlaIV GGNNCC 4 cut(s) 79, 121, 669, 681
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 2 cut(s) 168, 853
NspI RCATGY 3 cut(s) 640, 764, 787
PaeR7I CTCGAG 1 cut(s) 622
PciI ACATGT 1 cut(s) 760
PdmI GAANNNNTTC 2 cut(s) 425, 589
PkrI GCNGC 6 cut(s) 32, 49, 52, 55, 705, 738
PleI GAGTC 1 cut(s) 614
PpsI GAGTC 1 cut(s) 614
Ppu21I YACGTR 1 cut(s) 339
PpuMI RGGWCCY 1 cut(s) 78
PscI ACATGT 1 cut(s) 760
Psp5II RGGWCCY 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 715
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 4 cut(s) 79, 121, 669, 681
PspPI GGNCC 3 cut(s) 78, 517, 803
PspPPI RGGWCCY 1 cut(s) 78
PspXI VCTCGAGB 1 cut(s) 622
PstI CTGCAG 1 cut(s) 696
PsuI RGATCY 2 cut(s) 261, 647
RsaI GTAC 3 cut(s) 444, 681, 768
RsaNI GTAC 3 cut(s) 443, 680, 767
RseI CAYNNNNRTG 2 cut(s) 434, 731
SaqAI TTAA 1 cut(s) 771
SatI GCNGC 6 cut(s) 31, 48, 51, 54, 704, 737
Sau3AI GATC 4 cut(s) 261, 374, 413, 647
Sau96I GGNCC 3 cut(s) 78, 517, 803
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 1 cut(s) 614
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 5 cut(s) 246, 270, 517, 723, 728
SfcI CTRYAG 1 cut(s) 692
Sfr274I CTCGAG 1 cut(s) 622
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 1 cut(s) 78
SlaI CTCGAG 1 cut(s) 622
SmiMI CAYNNNNRTG 2 cut(s) 434, 731
SmlI CTYRAG 1 cut(s) 622
SmoI CTYRAG 1 cut(s) 622
Sse9I AATT 3 cut(s) 155, 321, 535
SspMI CTAG 1 cut(s) 186
StyD4I CCNGG 1 cut(s) 84
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 1 cut(s) 859
TaiI ACGT 2 cut(s) 341, 363
TaqI TCGA 1 cut(s) 623
TasI AATT 3 cut(s) 155, 321, 535
TatI WGTACW 2 cut(s) 442, 766
TauI GCSGC 5 cut(s) 33, 50, 53, 56, 739
Tru1I TTAA 1 cut(s) 771
Tru9I TTAA 1 cut(s) 771
TscAI CASTG 1 cut(s) 494
TseFI GTSAC 2 cut(s) 168, 853
TseI GCWGC 1 cut(s) 703
Tsp45I GTSAC 2 cut(s) 168, 853
TspDTI ATGAA 2 cut(s) 312, 452
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 78
XapI RAATTY 2 cut(s) 321, 535
XceI RCATGY 3 cut(s) 640, 764, 787
XcmI CCANNNNNNNNNTGG 2 cut(s) 273, 527
XhoI CTCGAG 1 cut(s) 622
XmaJI CCTAGG 1 cut(s) 185
XmnI GAANNNNTTC 2 cut(s) 425, 589
XspI CTAG 1 cut(s) 186
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.