Mei2-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1957 |
| Acc65I | GGTACC | 1 cut(s) | 1178 |
| AccB1I | GGYRCC | 1 cut(s) | 1178 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1678, 1766 |
| AccBSI | CCGCTC | 1 cut(s) | 548 |
| AccI | GTMKAC | 1 cut(s) | 1263 |
| AccII | CGCG | 1 cut(s) | 2067 |
| AciI | CCGC | 7 cut(s) | 432, 548, 1063, 1786, 1996, 2065, 2067 |
| AclWI | GGATC | 3 cut(s) | 272, 928, 1690 |
| AcoI | YGGCCR | 1 cut(s) | 1574 |
| AcsI | RAATTY | 7 cut(s) | 190, 301, 965, 1483, 1489, 2371, 2467 |
| AcuI | CTGAAG | 2 cut(s) | 60, 810 |
| AfaI | GTAC | 6 cut(s) | 112, 1180, 1922, 2117, 2164, 2180 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1243, 1244, 1678, 1766, 2425 |
| AhlI | ACTAGT | 1 cut(s) | 1451 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1534, 1566 |
| Alw21I | GWGCWC | 4 cut(s) | 161, 1015, 1078, 1652 |
| Alw26I | GTCTC | 4 cut(s) | 772, 949, 2244, 2429 |
| AlwI | GGATC | 3 cut(s) | 272, 928, 1690 |
| AoxI | GGCC | 4 cut(s) | 45, 1574, 2405, 2428 |
| ApeKI | GCWGC | 9 cut(s) | 379, 992, 1001, 1093, 1406, 1952, 2138, 2348, 2488 |
| ApoI | RAATTY | 7 cut(s) | 190, 301, 965, 1483, 1489, 2371, 2467 |
| Asp718I | GGTACC | 1 cut(s) | 1178 |
| AspS9I | GGNCC | 6 cut(s) | 45, 594, 1460, 1517, 1946, 2428 |
| AsuC2I | CCSGG | 2 cut(s) | 260, 2159 |
| AsuHPI | GGTGA | 6 cut(s) | 1146, 1487, 1728, 2147, 2453, 2522 |
| AvaII | GGWCC | 4 cut(s) | 594, 1460, 1517, 1946 |
| BaeGI | GKGCMC | 1 cut(s) | 1189 |
| BalI | TGGCCA | 1 cut(s) | 1576 |
| BanI | GGYRCC | 1 cut(s) | 1178 |
| BanII | GRGCYC | 4 cut(s) | 161, 1078, 1560, 1652 |
| BbsI | GAAGAC | 4 cut(s) | 339, 1557, 1841, 2079 |
| Bbv12I | GWGCWC | 4 cut(s) | 161, 1015, 1078, 1652 |
| BbvI | GCAGC | 9 cut(s) | 366, 979, 1013, 1105, 1418, 1964, 2125, 2335, 2500 |
| BccI | CCATC | 8 cut(s) | 67, 394, 494, 1472, 1680, 1699, 1967, 2489 |
| BceAI | ACGGC | 1 cut(s) | 2501 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 145, 179 |
| BciVI | GTATCC | 1 cut(s) | 2345 |
| BclI | TGATCA | 1 cut(s) | 2094 |
| BcnI | CCSGG | 2 cut(s) | 260, 2159 |
| BcoDI | GTCTC | 4 cut(s) | 772, 949, 2244, 2429 |
| BcuI | ACTAGT | 1 cut(s) | 1451 |
| BfaI | CTAG | 7 cut(s) | 75, 144, 245, 384, 392, 1452, 1881 |
| BfmI | CTRYAG | 4 cut(s) | 993, 1260, 1950, 2142 |
| BfuAI | ACCTGC | 1 cut(s) | 1957 |
| BfuI | GTATCC | 1 cut(s) | 2345 |
| BglII | AGATCT | 1 cut(s) | 644 |
| BlpI | GCTNAGC | 1 cut(s) | 488 |
| Bme18I | GGWCC | 4 cut(s) | 594, 1460, 1517, 1946 |
| BmgT120I | GGNCC | 6 cut(s) | 45, 594, 1460, 1517, 1946, 2428 |
| BmiI | GGNNCC | 4 cut(s) | 46, 1180, 1462, 1519 |
| BmrI | ACTGGG | 1 cut(s) | 1516 |
| BmsI | GCATC | 5 cut(s) | 140, 592, 862, 884, 1080 |
| BmuI | ACTGGG | 1 cut(s) | 1516 |
| BpiI | GAAGAC | 4 cut(s) | 339, 1557, 1841, 2079 |
| BpmI | CTGGAG | 3 cut(s) | 582, 1158, 2446 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1434 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 488 |
| BpuEI | CTTGAG | 1 cut(s) | 796 |
| BpuMI | CCSGG | 2 cut(s) | 260, 2159 |
| BsaAI | YACGTR | 1 cut(s) | 2333 |
| BsaBI | GATNNNNATC | 3 cut(s) | 366, 2151, 2547 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1153, 1183 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1243, 1244, 1678, 1766, 2425 |
| Bse118I | RCCGGY | 2 cut(s) | 1641, 2403 |
| Bse1I | ACTGG | 4 cut(s) | 1522, 1948, 2325, 2443 |
| Bse3DI | GCAATG | 3 cut(s) | 161, 756, 2215 |
| Bse8I | GATNNNNATC | 3 cut(s) | 366, 2151, 2547 |
| BseGI | GGATG | 7 cut(s) | 568, 583, 877, 1594, 1745, 2103, 2548 |
| BseJI | GATNNNNATC | 3 cut(s) | 366, 2151, 2547 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1243, 1244, 1678, 1766, 2425 |
| BseMI | GCAATG | 3 cut(s) | 161, 756, 2215 |
| BseMII | CTCAG | 5 cut(s) | 108, 1244, 1425, 1545, 1672 |
| BseNI | ACTGG | 4 cut(s) | 1522, 1948, 2325, 2443 |
| BseRI | GAGGAG | 2 cut(s) | 410, 1161 |
| BseSI | GKGCMC | 1 cut(s) | 1189 |
| BseXI | GCAGC | 9 cut(s) | 366, 979, 1013, 1105, 1418, 1964, 2125, 2335, 2500 |
| BseYI | CCCAGC | 2 cut(s) | 1034, 1058 |
| Bsh1236I | CGCG | 1 cut(s) | 2067 |
| BshFI | GGCC | 4 cut(s) | 47, 1576, 2407, 2430 |
| BshNI | GGYRCC | 1 cut(s) | 1178 |
| BsiHKAI | GWGCWC | 4 cut(s) | 161, 1015, 1078, 1652 |
| BsiSI | CCGG | 4 cut(s) | 260, 1642, 2158, 2404 |
| BslFI | GGGAC | 5 cut(s) | 1219, 1446, 1503, 1605, 1632 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1243, 1244, 1678, 1766, 2425 |
| BsmAI | GTCTC | 4 cut(s) | 772, 949, 2244, 2429 |
| BsmBI | CGTCTC | 1 cut(s) | 772 |
| BsmFI | GGGAC | 5 cut(s) | 1219, 1446, 1503, 1605, 1632 |
| BsmI | GAATGC | 2 cut(s) | 1417, 1840 |
| BsnI | GGCC | 4 cut(s) | 47, 1576, 2407, 2430 |
| Bsp1286I | GDGCHC | 6 cut(s) | 161, 1015, 1078, 1189, 1560, 1652 |
| Bsp143I | GATC | 9 cut(s) | 277, 367, 412, 644, 734, 933, 1682, 1978, 2094 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 488 |
| BspACI | CCGC | 7 cut(s) | 432, 548, 1063, 1786, 1996, 2065, 2067 |
| BspANI | GGCC | 4 cut(s) | 47, 1576, 2407, 2430 |
| BspCNI | CTCAG | 5 cut(s) | 109, 1245, 1426, 1546, 1671 |
| BspFNI | CGCG | 1 cut(s) | 2067 |
| BspHI | TCATGA | 1 cut(s) | 1752 |
| BspLI | GGNNCC | 4 cut(s) | 46, 1180, 1462, 1519 |
| BspMAI | CTGCAG | 2 cut(s) | 997, 1954 |
| BspMI | ACCTGC | 1 cut(s) | 1957 |
| BspPI | GGATC | 3 cut(s) | 272, 928, 1690 |
| BspQI | GCTCTTC | 1 cut(s) | 540 |
| BspT107I | GGYRCC | 1 cut(s) | 1178 |
| BsrBI | CCGCTC | 1 cut(s) | 548 |
| BsrDI | GCAATG | 3 cut(s) | 161, 756, 2215 |
| BsrFI | RCCGGY | 2 cut(s) | 1641, 2403 |
| BsrI | ACTGG | 4 cut(s) | 1522, 1948, 2325, 2443 |
| BssAI | RCCGGY | 2 cut(s) | 1641, 2403 |
| BssMI | GATC | 9 cut(s) | 277, 367, 412, 644, 734, 933, 1682, 1978, 2094 |
| BssT1I | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1924 |
| Bst4CI | ACNGT | 3 cut(s) | 556, 2078, 2303 |
| Bst6I | CTCTTC | 3 cut(s) | 399, 540, 2420 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 128, 755 |
| BstBAI | YACGTR | 1 cut(s) | 2333 |
| BstC8I | GCNNGC | 8 cut(s) | 236, 349, 691, 695, 742, 756, 1840, 2405 |
| BstDEI | CTNAG | 8 cut(s) | 117, 371, 488, 1100, 1253, 1434, 1554, 1658 |
| BstDSI | CCRYGG | 1 cut(s) | 2065 |
| BstF5I | GGATG | 7 cut(s) | 568, 583, 877, 1594, 1745, 2103, 2548 |
| BstFNI | CGCG | 1 cut(s) | 2067 |
| BstKTI | GATC | 9 cut(s) | 280, 370, 415, 647, 737, 936, 1685, 1981, 2097 |
| BstMAI | GTCTC | 4 cut(s) | 772, 949, 2244, 2429 |
| BstMBI | GATC | 9 cut(s) | 277, 367, 412, 644, 734, 933, 1682, 1978, 2094 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 128, 429, 746, 755, 998, 1001, 1010, 2321, 2404 |
| BstSFI | CTRYAG | 4 cut(s) | 993, 1260, 1950, 2142 |
| BstSLI | GKGCMC | 1 cut(s) | 1189 |
| BstUI | CGCG | 1 cut(s) | 2067 |
| BstV1I | GCAGC | 9 cut(s) | 366, 979, 1013, 1105, 1418, 1964, 2125, 2335, 2500 |
| BstV2I | GAAGAC | 4 cut(s) | 339, 1557, 1841, 2079 |
| BstX2I | RGATCY | 4 cut(s) | 277, 644, 933, 1682 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1182, 2438 |
| BstYI | RGATCY | 4 cut(s) | 277, 644, 933, 1682 |
| BsuI | GTATCC | 1 cut(s) | 2345 |
| BsuRI | GGCC | 4 cut(s) | 47, 1576, 2407, 2430 |
| BtgI | CCRYGG | 1 cut(s) | 2065 |
| BtsCI | GGATG | 7 cut(s) | 568, 583, 877, 1594, 1745, 2103, 2548 |
| BtsI | GCAGTG | 2 cut(s) | 430, 744 |
| BtsIMutI | CAGTG | 7 cut(s) | 430, 744, 885, 1941, 1969, 2308, 2318 |
| BveI | ACCTGC | 1 cut(s) | 1957 |
| Cac8I | GCNNGC | 8 cut(s) | 236, 349, 691, 695, 742, 756, 1840, 2405 |
| CciI | TCATGA | 1 cut(s) | 1752 |
| Cfr10I | RCCGGY | 2 cut(s) | 1641, 2403 |
| Cfr13I | GGNCC | 6 cut(s) | 45, 594, 1460, 1517, 1946, 2428 |
| Cfr42I | CCGCGG | 1 cut(s) | 2068 |
| CseI | GACGC | 1 cut(s) | 203 |
| Csp6I | GTAC | 6 cut(s) | 111, 1179, 1921, 2116, 2163, 2179 |
| CviAII | CATG | 9 cut(s) | 96, 128, 179, 1307, 1676, 1753, 1774, 1804, 1813 |
| CviQI | GTAC | 6 cut(s) | 111, 1179, 1921, 2116, 2163, 2179 |
| DdeI | CTNAG | 8 cut(s) | 117, 371, 488, 1100, 1253, 1434, 1554, 1658 |
| DpnI | GATC | 9 cut(s) | 279, 369, 414, 646, 736, 935, 1684, 1980, 2096 |
| DpnII | GATC | 9 cut(s) | 277, 367, 412, 644, 734, 933, 1682, 1978, 2094 |
| EaeI | YGGCCR | 1 cut(s) | 1574 |
| Eam1104I | CTCTTC | 3 cut(s) | 399, 540, 2420 |
| EarI | CTCTTC | 3 cut(s) | 399, 540, 2420 |
| EciI | GGCGGA | 1 cut(s) | 447 |
| Ecl136II | GAGCTC | 3 cut(s) | 159, 1076, 1650 |
| Eco130I | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1924 |
| Eco24I | GRGCYC | 4 cut(s) | 161, 1078, 1560, 1652 |
| Eco32I | GATATC | 3 cut(s) | 670, 730, 838 |
| Eco47I | GGWCC | 4 cut(s) | 594, 1460, 1517, 1946 |
| Eco53kI | GAGCTC | 3 cut(s) | 159, 1076, 1650 |
| Eco57I | CTGAAG | 2 cut(s) | 60, 810 |
| EcoICRI | GAGCTC | 3 cut(s) | 159, 1076, 1650 |
| EcoO109I | RGGNCCY | 4 cut(s) | 45, 594, 1460, 1517 |
| EcoRI | GAATTC | 1 cut(s) | 2371 |
| EcoRV | GATATC | 3 cut(s) | 670, 730, 838 |
| EcoT14I | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1924 |
| EcoT22I | ATGCAT | 3 cut(s) | 133, 877, 2483 |
| EcoT38I | GRGCYC | 4 cut(s) | 161, 1078, 1560, 1652 |
| ErhI | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1924 |
| Esp3I | CGTCTC | 1 cut(s) | 772 |
| FaeI | CATG | 9 cut(s) | 99, 131, 182, 1310, 1679, 1756, 1777, 1807, 1816 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 267, 299, 450, 482, 837, 869, 2337, 2369 |
| FaqI | GGGAC | 5 cut(s) | 1219, 1446, 1503, 1605, 1632 |
| FatI | CATG | 9 cut(s) | 95, 127, 178, 1306, 1675, 1752, 1773, 1803, 1812 |
| FauI | CCCGC | 3 cut(s) | 541, 1989, 2060 |
| FauNDI | CATATG | 2 cut(s) | 1471, 2477 |
| FbaI | TGATCA | 1 cut(s) | 2094 |
| FblI | GTMKAC | 1 cut(s) | 1263 |
| FokI | GGATG | 7 cut(s) | 555, 570, 884, 1581, 1732, 2090, 2555 |
| FriOI | GRGCYC | 4 cut(s) | 161, 1078, 1560, 1652 |
| FspBI | CTAG | 7 cut(s) | 75, 144, 245, 384, 392, 1452, 1881 |
| GsaI | CCCAGC | 2 cut(s) | 1038, 1062 |
| GsuI | CTGGAG | 3 cut(s) | 582, 1158, 2446 |
| HaeIII | GGCC | 4 cut(s) | 47, 1576, 2407, 2430 |
| HapII | CCGG | 4 cut(s) | 260, 1642, 2158, 2404 |
| HgaI | GACGC | 1 cut(s) | 203 |
| Hin1II | CATG | 9 cut(s) | 99, 131, 182, 1310, 1679, 1756, 1777, 1807, 1816 |
| HincII | GTYRAC | 1 cut(s) | 256 |
| HindII | GTYRAC | 1 cut(s) | 256 |
| HindIII | AAGCTT | 1 cut(s) | 1049 |
| HpaII | CCGG | 4 cut(s) | 260, 1642, 2158, 2404 |
| HphI | GGTGA | 6 cut(s) | 1146, 1487, 1728, 2147, 2453, 2522 |
| Hpy166II | GTNNAC | 7 cut(s) | 256, 1154, 1264, 1613, 1664, 1921, 2118 |
| Hpy188III | TCNNGA | 9 cut(s) | 323, 392, 1106, 1433, 1706, 1753, 2463, 2540, 2552 |
| Hpy8I | GTNNAC | 7 cut(s) | 256, 1154, 1264, 1613, 1664, 1921, 2118 |
| HpyCH4III | ACNGT | 3 cut(s) | 556, 2078, 2303 |
| HpyCH4IV | ACGT | 2 cut(s) | 984, 2332 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 128, 429, 746, 755, 998, 1001, 1010, 2321, 2404 |
| HpyF3I | CTNAG | 8 cut(s) | 117, 371, 488, 1100, 1253, 1434, 1554, 1658 |
| HpySE526I | ACGT | 2 cut(s) | 984, 2332 |
| Hsp92II | CATG | 9 cut(s) | 99, 131, 182, 1310, 1679, 1756, 1777, 1807, 1816 |
| KpnI | GGTACC | 1 cut(s) | 1182 |
| KroI | GCCGGC | 1 cut(s) | 2403 |
| KroNI | GCCGGC | 1 cut(s) | 2405 |
| Ksp22I | TGATCA | 1 cut(s) | 2094 |
| KspI | CCGCGG | 1 cut(s) | 2068 |
| Kzo9I | GATC | 9 cut(s) | 277, 367, 412, 644, 734, 933, 1682, 1978, 2094 |
| LguI | GCTCTTC | 1 cut(s) | 540 |
| LmnI | GCTCC | 8 cut(s) | 205, 545, 580, 1023, 1073, 1403, 1655, 1693 |
| Lsp1109I | GCAGC | 9 cut(s) | 366, 979, 1013, 1105, 1418, 1964, 2125, 2335, 2500 |
| LweI | GCATC | 5 cut(s) | 140, 592, 862, 884, 1080 |
| MaeI | CTAG | 7 cut(s) | 75, 144, 245, 384, 392, 1452, 1881 |
| MaeII | ACGT | 2 cut(s) | 984, 2332 |
| MaeIII | GTNAC | 3 cut(s) | 1382, 2356, 2441 |
| MalI | GATC | 9 cut(s) | 279, 369, 414, 646, 736, 935, 1684, 1980, 2096 |
| MbiI | CCGCTC | 1 cut(s) | 548 |
| MboI | GATC | 9 cut(s) | 277, 367, 412, 644, 734, 933, 1682, 1978, 2094 |
| MflI | RGATCY | 4 cut(s) | 277, 644, 933, 1682 |
| MhlI | GDGCHC | 6 cut(s) | 161, 1015, 1078, 1189, 1560, 1652 |
| MlsI | TGGCCA | 1 cut(s) | 1576 |
| MluNI | TGGCCA | 1 cut(s) | 1576 |
| MlyI | GAGTC | 5 cut(s) | 626, 1264, 1487, 1999, 2353 |
| MmeI | TCCRAC | 2 cut(s) | 1162, 1325 |
| Mox20I | TGGCCA | 1 cut(s) | 1576 |
| Mph1103I | ATGCAT | 3 cut(s) | 133, 877, 2483 |
| MroNI | GCCGGC | 1 cut(s) | 2403 |
| MscI | TGGCCA | 1 cut(s) | 1576 |
| MslI | CAYNNNNRTG | 3 cut(s) | 573, 749, 2231 |
| Msp20I | TGGCCA | 1 cut(s) | 1576 |
| MspA1I | CMGCKG | 1 cut(s) | 2067 |
| MspI | CCGG | 4 cut(s) | 260, 1642, 2158, 2404 |
| Mva1269I | GAATGC | 2 cut(s) | 1417, 1840 |
| MvnI | CGCG | 1 cut(s) | 2067 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 128, 429, 746, 755, 998, 1001, 1010, 2321, 2404 |
| NaeI | GCCGGC | 1 cut(s) | 2405 |
| NciI | CCSGG | 2 cut(s) | 260, 2159 |
| NdeI | CATATG | 2 cut(s) | 1471, 2477 |
| NdeII | GATC | 9 cut(s) | 277, 367, 412, 644, 734, 933, 1682, 1978, 2094 |
| NgoMIV | GCCGGC | 1 cut(s) | 2403 |
| NlaIII | CATG | 9 cut(s) | 99, 131, 182, 1310, 1679, 1756, 1777, 1807, 1816 |
| NlaIV | GGNNCC | 4 cut(s) | 46, 1180, 1462, 1519 |
| NmuCI | GTSAC | 2 cut(s) | 2356, 2441 |
| NsiI | ATGCAT | 3 cut(s) | 133, 877, 2483 |
| PagI | TCATGA | 1 cut(s) | 1752 |
| PciSI | GCTCTTC | 1 cut(s) | 540 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1135 |
| PctI | GAATGC | 2 cut(s) | 1417, 1840 |
| PdiI | GCCGGC | 1 cut(s) | 2405 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1678, 1766 |
| PfoI | TCCNGGA | 1 cut(s) | 1852 |
| PleI | GAGTC | 5 cut(s) | 626, 1263, 1486, 1998, 2353 |
| PpsI | GAGTC | 5 cut(s) | 626, 1263, 1486, 1998, 2353 |
| Ppu21I | YACGTR | 1 cut(s) | 2333 |
| PpuMI | RGGWCCY | 3 cut(s) | 594, 1460, 1517 |
| Psp124BI | GAGCTC | 3 cut(s) | 161, 1078, 1652 |
| Psp5II | RGGWCCY | 3 cut(s) | 594, 1460, 1517 |
| PspFI | CCCAGC | 2 cut(s) | 1034, 1058 |
| PspN4I | GGNNCC | 4 cut(s) | 46, 1180, 1462, 1519 |
| PspPI | GGNCC | 6 cut(s) | 45, 594, 1460, 1517, 1946, 2428 |
| PspPPI | RGGWCCY | 3 cut(s) | 594, 1460, 1517 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 667, 699 |
| PstI | CTGCAG | 2 cut(s) | 997, 1954 |
| PsuI | RGATCY | 4 cut(s) | 277, 644, 933, 1682 |
| RsaI | GTAC | 6 cut(s) | 112, 1180, 1922, 2117, 2164, 2180 |
| RsaNI | GTAC | 6 cut(s) | 111, 1179, 1921, 2116, 2163, 2179 |
| RseI | CAYNNNNRTG | 3 cut(s) | 573, 749, 2231 |
| SacI | GAGCTC | 3 cut(s) | 161, 1078, 1652 |
| SacII | CCGCGG | 1 cut(s) | 2068 |
| SapI | GCTCTTC | 1 cut(s) | 540 |
| Sau3AI | GATC | 9 cut(s) | 277, 367, 412, 644, 734, 933, 1682, 1978, 2094 |
| Sau96I | GGNCC | 6 cut(s) | 45, 594, 1460, 1517, 1946, 2428 |
| SchI | GAGTC | 5 cut(s) | 626, 1264, 1487, 1999, 2353 |
| SduI | GDGCHC | 6 cut(s) | 161, 1015, 1078, 1189, 1560, 1652 |
| SfaNI | GCATC | 5 cut(s) | 140, 592, 862, 884, 1080 |
| SfcI | CTRYAG | 4 cut(s) | 993, 1260, 1950, 2142 |
| Sfr303I | CCGCGG | 1 cut(s) | 2068 |
| SgrBI | CCGCGG | 1 cut(s) | 2068 |
| SinI | GGWCC | 4 cut(s) | 594, 1460, 1517, 1946 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 573, 749, 2231 |
| SmlI | CTYRAG | 1 cut(s) | 775 |
| SmoI | CTYRAG | 1 cut(s) | 775 |
| SpeI | ACTAGT | 1 cut(s) | 1451 |
| SsiI | CCGC | 7 cut(s) | 432, 548, 1063, 1786, 1996, 2065, 2067 |
| SspI | AATATT | 1 cut(s) | 70 |
| SspMI | CTAG | 7 cut(s) | 75, 144, 245, 384, 392, 1452, 1881 |
| SstI | GAGCTC | 3 cut(s) | 161, 1078, 1652 |
| StyI | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1924 |
| TaaI | ACNGT | 3 cut(s) | 556, 2078, 2303 |
| TaiI | ACGT | 2 cut(s) | 987, 2335 |
| TaqI | TCGA | 4 cut(s) | 155, 898, 1078, 2190 |
| TaqII | GACCGA | 1 cut(s) | 52 |
| TatI | WGTACW | 2 cut(s) | 2115, 2178 |
| TauI | GCSGC | 2 cut(s) | 1065, 1788 |
| TscAI | CASTG | 7 cut(s) | 430, 751, 885, 1948, 1969, 2308, 2325 |
| TseFI | GTSAC | 2 cut(s) | 2356, 2441 |
| TseI | GCWGC | 9 cut(s) | 379, 992, 1001, 1093, 1406, 1952, 2138, 2348, 2488 |
| Tsp45I | GTSAC | 2 cut(s) | 2356, 2441 |
| TspGWI | ACGGA | 1 cut(s) | 1587 |
| TspRI | CASTG | 7 cut(s) | 430, 751, 885, 1948, 1969, 2308, 2325 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1678, 1766 |
| VpaK11BI | GGWCC | 4 cut(s) | 594, 1460, 1517, 1946 |
| XapI | RAATTY | 7 cut(s) | 190, 301, 965, 1483, 1489, 2371, 2467 |
| XbaI | TCTAGA | 1 cut(s) | 391 |
| XmiI | GTMKAC | 1 cut(s) | 1263 |
| XspI | CTAG | 7 cut(s) | 75, 144, 245, 384, 392, 1452, 1881 |
| Zsp2I | ATGCAT | 3 cut(s) | 133, 877, 2483 |