Mei2-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 475, 529 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 83 |
| Acc36I | ACCTGC | 3 cut(s) | 475, 529, 2267 |
| Acc65I | GGTACC | 1 cut(s) | 859 |
| AccB1I | GGYRCC | 1 cut(s) | 859 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 65, 263 |
| AccBSI | CCGCTC | 1 cut(s) | 1862 |
| AccI | GTMKAC | 1 cut(s) | 2182 |
| AccII | CGCG | 1 cut(s) | 1530 |
| AciI | CCGC | 5 cut(s) | 114, 143, 1528, 1862, 2203 |
| AcoI | YGGCCR | 5 cut(s) | 1525, 1558, 2293, 2383, 2585 |
| AcsI | RAATTY | 4 cut(s) | 130, 911, 1532, 1576 |
| AcuI | CTGAAG | 7 cut(s) | 399, 720, 990, 999, 1523, 2022, 2345 |
| AfaI | GTAC | 2 cut(s) | 861, 2477 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 65, 149, 263, 552, 1049, 1231, 2212, 2213, 2375 |
| AflIII | ACRYGT | 2 cut(s) | 668, 1897 |
| AjiI | CACGTC | 1 cut(s) | 320 |
| AjnI | CCWGG | 4 cut(s) | 148, 658, 982, 1286 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1428, 1460, 2503, 2535 |
| Alw26I | GTCTC | 2 cut(s) | 91, 424 |
| Ama87I | CYCGRG | 2 cut(s) | 1737, 1773 |
| ApeKI | GCWGC | 4 cut(s) | 682, 826, 1733, 2162 |
| ApoI | RAATTY | 4 cut(s) | 130, 911, 1532, 1576 |
| AseI | ATTAAT | 1 cut(s) | 1589 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1239, 1638 |
| Asp718I | GGTACC | 1 cut(s) | 859 |
| AspLEI | GCGC | 1 cut(s) | 921 |
| AspS9I | GGNCC | 6 cut(s) | 269, 410, 1040, 1051, 1609, 2489 |
| AsuC2I | CCSGG | 1 cut(s) | 2114 |
| AsuHPI | GGTGA | 7 cut(s) | 468, 567, 965, 1559, 2071, 2192, 2228 |
| AvaI | CYCGRG | 2 cut(s) | 1737, 1773 |
| AvaII | GGWCC | 1 cut(s) | 269 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2467, 2500 |
| BalI | TGGCCA | 3 cut(s) | 2295, 2385, 2587 |
| BamHI | GGATCC | 2 cut(s) | 637, 1282 |
| BanI | GGYRCC | 1 cut(s) | 859 |
| BanII | GRGCYC | 1 cut(s) | 2068 |
| BbsI | GAAGAC | 4 cut(s) | 980, 1376, 1556, 1767 |
| BbvCI | CCTCAGC | 2 cut(s) | 104, 753 |
| BbvI | GCAGC | 4 cut(s) | 694, 838, 1745, 2174 |
| BccI | CCATC | 8 cut(s) | 209, 213, 265, 481, 580, 875, 1109, 1469 |
| BceAI | ACGGC | 2 cut(s) | 827, 1545 |
| BciT130I | CCWGG | 4 cut(s) | 150, 660, 984, 1288 |
| BciVI | GTATCC | 1 cut(s) | 169 |
| BclI | TGATCA | 2 cut(s) | 1513, 2133 |
| BcnI | CCSGG | 1 cut(s) | 2114 |
| BcoDI | GTCTC | 2 cut(s) | 91, 424 |
| BfaI | CTAG | 9 cut(s) | 707, 819, 830, 1857, 1868, 1955, 2096, 2481, 2544 |
| BfmI | CTRYAG | 3 cut(s) | 72, 864, 1029 |
| BfuAI | ACCTGC | 3 cut(s) | 475, 529, 2267 |
| BfuI | GTATCC | 1 cut(s) | 169 |
| BisI | GCNGC | 6 cut(s) | 683, 827, 1528, 1734, 1863, 2163 |
| BlpI | GCTNAGC | 1 cut(s) | 1744 |
| BlsI | GCNGC | 6 cut(s) | 684, 828, 1529, 1735, 1864, 2164 |
| Bme1390I | CCNGG | 5 cut(s) | 150, 660, 984, 1288, 2114 |
| Bme18I | GGWCC | 1 cut(s) | 269 |
| BmeT110I | CYCGRG | 2 cut(s) | 1737, 1773 |
| BmgBI | CACGTC | 1 cut(s) | 320 |
| BmgT120I | GGNCC | 6 cut(s) | 269, 410, 1040, 1051, 1609, 2489 |
| BmiI | GGNNCC | 5 cut(s) | 639, 861, 1284, 1318, 1926 |
| BmrFI | CCNGG | 5 cut(s) | 150, 660, 984, 1288, 2114 |
| BmsI | GCATC | 6 cut(s) | 802, 824, 1405, 1542, 1696, 2355 |
| BpiI | GAAGAC | 4 cut(s) | 980, 1376, 1556, 1767 |
| BpmI | CTGGAG | 5 cut(s) | 285, 348, 642, 1303, 1911 |
| Bpu10I | CCTNAGC | 2 cut(s) | 104, 753 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1744 |
| BpuEI | CTTGAG | 1 cut(s) | 295 |
| BpuMI | CCSGG | 1 cut(s) | 2114 |
| Bsa29I | ATCGAT | 1 cut(s) | 2102 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1515, 2307 |
| BsaJI | CCNNGG | 4 cut(s) | 1286, 1726, 1833, 2154 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 637, 667 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 65, 149, 263, 552, 1049, 1231, 2212, 2213, 2375 |
| Bse118I | RCCGGY | 1 cut(s) | 2017 |
| Bse1I | ACTGG | 7 cut(s) | 496, 1088, 1363, 1818, 1857, 1948, 2506 |
| Bse3DI | GCAATG | 2 cut(s) | 1944, 2254 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1515, 2307 |
| BseBI | CCWGG | 4 cut(s) | 150, 660, 984, 1288 |
| BseCI | ATCGAT | 1 cut(s) | 2102 |
| BseDI | CCNNGG | 4 cut(s) | 1286, 1726, 1833, 2154 |
| BseGI | GGATG | 3 cut(s) | 201, 205, 1711 |
| BseJI | GATNNNNATC | 2 cut(s) | 1515, 2307 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 65, 149, 263, 552, 1049, 1231, 2212, 2213, 2375 |
| BseMI | GCAATG | 2 cut(s) | 1944, 2254 |
| BseMII | CTCAG | 2 cut(s) | 118, 744 |
| BseNI | ACTGG | 7 cut(s) | 496, 1088, 1363, 1818, 1857, 1948, 2506 |
| BseRI | GAGGAG | 3 cut(s) | 645, 1886, 2066 |
| BseXI | GCAGC | 4 cut(s) | 694, 838, 1745, 2174 |
| BsgI | GTGCAG | 5 cut(s) | 447, 453, 701, 1585, 1752 |
| Bsh1236I | CGCG | 1 cut(s) | 1530 |
| BshNI | GGYRCC | 1 cut(s) | 859 |
| BshVI | ATCGAT | 1 cut(s) | 2102 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1737, 1773 |
| BsiSI | CCGG | 3 cut(s) | 1232, 2018, 2114 |
| BslFI | GGGAC | 3 cut(s) | 547, 1246, 2156 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 65, 149, 263, 552, 1049, 1231, 2212, 2213, 2375 |
| BsmAI | GTCTC | 2 cut(s) | 91, 424 |
| BsmFI | GGGAC | 3 cut(s) | 547, 1246, 2156 |
| BsmI | GAATGC | 1 cut(s) | 2316 |
| BsoBI | CYCGRG | 2 cut(s) | 1737, 1773 |
| Bsp1286I | GDGCHC | 1 cut(s) | 2068 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1744 |
| BspACI | CCGC | 5 cut(s) | 114, 143, 1528, 1862, 2203 |
| BspCNI | CTCAG | 2 cut(s) | 117, 745 |
| BspDI | ATCGAT | 1 cut(s) | 2102 |
| BspFNI | CGCG | 1 cut(s) | 1530 |
| BspLI | GGNNCC | 5 cut(s) | 639, 861, 1284, 1318, 1926 |
| BspMAI | CTGCAG | 1 cut(s) | 76 |
| BspMI | ACCTGC | 3 cut(s) | 475, 529, 2267 |
| BspT107I | GGYRCC | 1 cut(s) | 859 |
| BsrBI | CCGCTC | 1 cut(s) | 1862 |
| BsrDI | GCAATG | 2 cut(s) | 1944, 2254 |
| BsrFI | RCCGGY | 1 cut(s) | 2017 |
| BsrI | ACTGG | 7 cut(s) | 496, 1088, 1363, 1818, 1857, 1948, 2506 |
| BssAI | RCCGGY | 1 cut(s) | 2017 |
| BssECI | CCNNGG | 4 cut(s) | 1286, 1726, 1833, 2154 |
| BssNAI | GTATAC | 1 cut(s) | 2183 |
| BssT1I | CCWWGG | 3 cut(s) | 1726, 1833, 2154 |
| Bst1107I | GTATAC | 1 cut(s) | 2183 |
| Bst2UI | CCWGG | 4 cut(s) | 150, 660, 984, 1288 |
| Bst4CI | ACNGT | 4 cut(s) | 401, 770, 1460, 2147 |
| Bst6I | CTCTTC | 2 cut(s) | 545, 1499 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 80, 2168 |
| BstC8I | GCNNGC | 7 cut(s) | 177, 181, 291, 750, 1137, 1801, 2383 |
| BstDEI | CTNAG | 6 cut(s) | 104, 753, 1537, 1744, 2394, 2531 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 550 |
| BstF5I | GGATG | 3 cut(s) | 201, 205, 1711 |
| BstFNI | CGCG | 1 cut(s) | 1530 |
| BstHHI | GCGC | 1 cut(s) | 921 |
| BstMAI | GTCTC | 2 cut(s) | 91, 424 |
| BstNI | CCWGG | 4 cut(s) | 150, 660, 984, 1288 |
| BstNSI | RCATGY | 5 cut(s) | 293, 508, 633, 672, 1901 |
| BstSCI | CCNGG | 5 cut(s) | 148, 658, 982, 1286, 2112 |
| BstSFI | CTRYAG | 3 cut(s) | 72, 864, 1029 |
| BstUI | CGCG | 1 cut(s) | 1530 |
| BstV1I | GCAGC | 4 cut(s) | 694, 838, 1745, 2174 |
| BstV2I | GAAGAC | 4 cut(s) | 980, 1376, 1556, 1767 |
| BstX2I | RGATCY | 7 cut(s) | 68, 637, 1282, 1361, 2023, 2462, 2567 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1670, 1701 |
| BstYI | RGATCY | 7 cut(s) | 68, 637, 1282, 1361, 2023, 2462, 2567 |
| BstZ17I | GTATAC | 1 cut(s) | 2183 |
| Bsu15I | ATCGAT | 1 cut(s) | 2102 |
| BsuI | GTATCC | 1 cut(s) | 169 |
| BsuTUI | ATCGAT | 1 cut(s) | 2102 |
| BtrI | CACGTC | 1 cut(s) | 320 |
| BtsCI | GGATG | 3 cut(s) | 201, 205, 1711 |
| BtsIMutI | CAGTG | 4 cut(s) | 775, 1299, 1465, 2152 |
| BveI | ACCTGC | 3 cut(s) | 475, 529, 2267 |
| Cac8I | GCNNGC | 7 cut(s) | 177, 181, 291, 750, 1137, 1801, 2383 |
| CfoI | GCGC | 1 cut(s) | 921 |
| Cfr10I | RCCGGY | 1 cut(s) | 2017 |
| Cfr13I | GGNCC | 6 cut(s) | 269, 410, 1040, 1051, 1609, 2489 |
| ClaI | ATCGAT | 1 cut(s) | 2102 |
| Csp6I | GTAC | 2 cut(s) | 860, 2476 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1233, 1268 |
| CviQI | GTAC | 2 cut(s) | 860, 2476 |
| DdeI | CTNAG | 6 cut(s) | 104, 753, 1537, 1744, 2394, 2531 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 83 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 83 |
| EaeI | YGGCCR | 5 cut(s) | 1525, 1558, 2293, 2383, 2585 |
| Eam1104I | CTCTTC | 2 cut(s) | 545, 1499 |
| EarI | CTCTTC | 2 cut(s) | 545, 1499 |
| EciI | GGCGGA | 1 cut(s) | 132 |
| Eco130I | CCWWGG | 3 cut(s) | 1726, 1833, 2154 |
| Eco147I | AGGCCT | 2 cut(s) | 226, 2231 |
| Eco24I | GRGCYC | 1 cut(s) | 2068 |
| Eco47I | GGWCC | 1 cut(s) | 269 |
| Eco57I | CTGAAG | 7 cut(s) | 399, 720, 990, 999, 1523, 2022, 2345 |
| Eco88I | CYCGRG | 2 cut(s) | 1737, 1773 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 550 |
| EcoO109I | RGGNCCY | 3 cut(s) | 269, 410, 2489 |
| EcoRI | GAATTC | 1 cut(s) | 1532 |
| EcoRII | CCWGG | 4 cut(s) | 148, 658, 982, 1286 |
| EcoT14I | CCWWGG | 3 cut(s) | 1726, 1833, 2154 |
| EcoT22I | ATGCAT | 1 cut(s) | 817 |
| EcoT38I | GRGCYC | 1 cut(s) | 2068 |
| ErhI | CCWWGG | 3 cut(s) | 1726, 1833, 2154 |
| FaqI | GGGAC | 3 cut(s) | 547, 1246, 2156 |
| FauI | CCCGC | 1 cut(s) | 107 |
| FbaI | TGATCA | 2 cut(s) | 1513, 2133 |
| FblI | GTMKAC | 1 cut(s) | 2182 |
| Fnu4HI | GCNGC | 6 cut(s) | 683, 827, 1528, 1734, 1863, 2163 |
| FokI | GGATG | 3 cut(s) | 188, 192, 1718 |
| FriOI | GRGCYC | 1 cut(s) | 2068 |
| Fsp4HI | GCNGC | 6 cut(s) | 683, 827, 1528, 1734, 1863, 2163 |
| FspBI | CTAG | 9 cut(s) | 707, 819, 830, 1857, 1868, 1955, 2096, 2481, 2544 |
| GlaI | GCGC | 1 cut(s) | 920 |
| GluI | GCNGC | 6 cut(s) | 683, 827, 1528, 1734, 1863, 2163 |
| GsuI | CTGGAG | 5 cut(s) | 285, 348, 642, 1303, 1911 |
| HapII | CCGG | 3 cut(s) | 1232, 2018, 2114 |
| HhaI | GCGC | 1 cut(s) | 921 |
| Hin6I | GCGC | 1 cut(s) | 919 |
| HinP1I | GCGC | 1 cut(s) | 919 |
| HincII | GTYRAC | 3 cut(s) | 404, 853, 1678 |
| HindII | GTYRAC | 3 cut(s) | 404, 853, 1678 |
| HindIII | AAGCTT | 2 cut(s) | 1248, 2237 |
| HinfI | GANTC | 8 cut(s) | 16, 43, 539, 1119, 1658, 1763, 2099, 2351 |
| HpaI | GTTAAC | 1 cut(s) | 853 |
| HpaII | CCGG | 3 cut(s) | 1232, 2018, 2114 |
| HphI | GGTGA | 7 cut(s) | 468, 567, 965, 1559, 2071, 2192, 2228 |
| Hpy166II | GTNNAC | 8 cut(s) | 404, 853, 976, 1456, 1678, 1972, 2183, 2561 |
| Hpy188I | TCNGA | 6 cut(s) | 1503, 1843, 2128, 2258, 2364, 2413 |
| Hpy8I | GTNNAC | 8 cut(s) | 404, 853, 976, 1456, 1678, 1972, 2183, 2561 |
| HpyCH4III | ACNGT | 4 cut(s) | 401, 770, 1460, 2147 |
| HpyCH4IV | ACGT | 3 cut(s) | 319, 1968, 2408 |
| HpyF3I | CTNAG | 6 cut(s) | 104, 753, 1537, 1744, 2394, 2531 |
| HpySE526I | ACGT | 3 cut(s) | 319, 1968, 2408 |
| HspAI | GCGC | 1 cut(s) | 919 |
| KpnI | GGTACC | 1 cut(s) | 863 |
| Ksp22I | TGATCA | 2 cut(s) | 1513, 2133 |
| KspAI | GTTAAC | 1 cut(s) | 853 |
| LmnI | GCTCC | 6 cut(s) | 145, 298, 367, 806, 1322, 1930 |
| Lsp1109I | GCAGC | 4 cut(s) | 694, 838, 1745, 2174 |
| LweI | GCATC | 6 cut(s) | 802, 824, 1405, 1542, 1696, 2355 |
| MaeI | CTAG | 9 cut(s) | 707, 819, 830, 1857, 1868, 1955, 2096, 2481, 2544 |
| MaeII | ACGT | 3 cut(s) | 319, 1968, 2408 |
| MaeIII | GTNAC | 6 cut(s) | 320, 1787, 1916, 1964, 2028, 2149 |
| MbiI | CCGCTC | 1 cut(s) | 1862 |
| MfeI | CAATTG | 2 cut(s) | 687, 2166 |
| MflI | RGATCY | 7 cut(s) | 68, 637, 1282, 1361, 2023, 2462, 2567 |
| MhlI | GDGCHC | 1 cut(s) | 2068 |
| MlsI | TGGCCA | 3 cut(s) | 2295, 2385, 2587 |
| MluNI | TGGCCA | 3 cut(s) | 2295, 2385, 2587 |
| MlyI | GAGTC | 1 cut(s) | 1757 |
| MmeI | TCCRAC | 3 cut(s) | 437, 727, 2440 |
| Mox20I | TGGCCA | 3 cut(s) | 2295, 2385, 2587 |
| Mph1103I | ATGCAT | 1 cut(s) | 817 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1239, 1638 |
| MscI | TGGCCA | 3 cut(s) | 2295, 2385, 2587 |
| MseI | TTAA | 6 cut(s) | 644, 696, 852, 1092, 1589, 2234 |
| MslI | CAYNNNNRTG | 3 cut(s) | 210, 1521, 1668 |
| Msp20I | TGGCCA | 3 cut(s) | 2295, 2385, 2587 |
| MspI | CCGG | 3 cut(s) | 1232, 2018, 2114 |
| MspR9I | CCNGG | 5 cut(s) | 150, 660, 984, 1288, 2114 |
| MunI | CAATTG | 2 cut(s) | 687, 2166 |
| Mva1269I | GAATGC | 1 cut(s) | 2316 |
| MvaI | CCWGG | 4 cut(s) | 150, 660, 984, 1288 |
| MvnI | CGCG | 1 cut(s) | 1530 |
| NciI | CCSGG | 1 cut(s) | 2114 |
| NlaIV | GGNNCC | 5 cut(s) | 639, 861, 1284, 1318, 1926 |
| NmuCI | GTSAC | 4 cut(s) | 320, 1787, 1964, 2028 |
| NsiI | ATGCAT | 1 cut(s) | 817 |
| NspI | RCATGY | 5 cut(s) | 293, 508, 633, 672, 1901 |
| PaeI | GCATGC | 1 cut(s) | 293 |
| PaeR7I | CTCGAG | 1 cut(s) | 1773 |
| PaqCI | CACCTGC | 2 cut(s) | 475, 529 |
| PceI | AGGCCT | 2 cut(s) | 226, 2231 |
| PciI | ACATGT | 2 cut(s) | 668, 1897 |
| PctI | GAATGC | 1 cut(s) | 2316 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1239, 1638 |
| PfeI | GAWTC | 7 cut(s) | 16, 43, 539, 1119, 1658, 2099, 2351 |
| PflFI | GACNNNGTC | 3 cut(s) | 559, 1258, 2539 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 65, 263 |
| PfoI | TCCNGGA | 2 cut(s) | 148, 658 |
| PkrI | GCNGC | 6 cut(s) | 684, 828, 1529, 1735, 1864, 2164 |
| PleI | GAGTC | 1 cut(s) | 1757 |
| PpsI | GAGTC | 1 cut(s) | 1757 |
| PpuMI | RGGWCCY | 1 cut(s) | 269 |
| PscI | ACATGT | 2 cut(s) | 668, 1897 |
| PshBI | ATTAAT | 1 cut(s) | 1589 |
| Psp5II | RGGWCCY | 1 cut(s) | 269 |
| Psp6I | CCWGG | 4 cut(s) | 148, 658, 982, 1286 |
| PspGI | CCWGG | 4 cut(s) | 148, 658, 982, 1286 |
| PspN4I | GGNNCC | 5 cut(s) | 639, 861, 1284, 1318, 1926 |
| PspPI | GGNCC | 6 cut(s) | 269, 410, 1040, 1051, 1609, 2489 |
| PspPPI | RGGWCCY | 1 cut(s) | 269 |
| PspXI | VCTCGAGB | 1 cut(s) | 1773 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 957, 989 |
| PstI | CTGCAG | 1 cut(s) | 76 |
| PsuI | RGATCY | 7 cut(s) | 68, 637, 1282, 1361, 2023, 2462, 2567 |
| PsyI | GACNNNGTC | 3 cut(s) | 559, 1258, 2539 |
| RsaI | GTAC | 2 cut(s) | 861, 2477 |
| RsaNI | GTAC | 2 cut(s) | 860, 2476 |
| RseI | CAYNNNNRTG | 3 cut(s) | 210, 1521, 1668 |
| SaqAI | TTAA | 6 cut(s) | 644, 696, 852, 1092, 1589, 2234 |
| SatI | GCNGC | 6 cut(s) | 683, 827, 1528, 1734, 1863, 2163 |
| Sau96I | GGNCC | 6 cut(s) | 269, 410, 1040, 1051, 1609, 2489 |
| SchI | GAGTC | 1 cut(s) | 1757 |
| ScrFI | CCNGG | 5 cut(s) | 150, 660, 984, 1288, 2114 |
| SduI | GDGCHC | 1 cut(s) | 2068 |
| SfaNI | GCATC | 6 cut(s) | 802, 824, 1405, 1542, 1696, 2355 |
| SfcI | CTRYAG | 3 cut(s) | 72, 864, 1029 |
| Sfr274I | CTCGAG | 1 cut(s) | 1773 |
| SinI | GGWCC | 1 cut(s) | 269 |
| SlaI | CTCGAG | 1 cut(s) | 1773 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 210, 1521, 1668 |
| SmlI | CTYRAG | 2 cut(s) | 310, 1773 |
| SmoI | CTYRAG | 2 cut(s) | 310, 1773 |
| SphI | GCATGC | 1 cut(s) | 293 |
| SseBI | AGGCCT | 2 cut(s) | 226, 2231 |
| SsiI | CCGC | 5 cut(s) | 114, 143, 1528, 1862, 2203 |
| SspMI | CTAG | 9 cut(s) | 707, 819, 830, 1857, 1868, 1955, 2096, 2481, 2544 |
| StuI | AGGCCT | 2 cut(s) | 226, 2231 |
| StyD4I | CCNGG | 5 cut(s) | 148, 658, 982, 1286, 2112 |
| StyI | CCWWGG | 3 cut(s) | 1726, 1833, 2154 |
| TaaI | ACNGT | 4 cut(s) | 401, 770, 1460, 2147 |
| TaiI | ACGT | 3 cut(s) | 322, 1971, 2411 |
| TaqI | TCGA | 4 cut(s) | 430, 1213, 1774, 2102 |
| TauI | GCSGC | 2 cut(s) | 1530, 1865 |
| TfiI | GAWTC | 7 cut(s) | 16, 43, 539, 1119, 1658, 2099, 2351 |
| Tru1I | TTAA | 6 cut(s) | 644, 696, 852, 1092, 1589, 2234 |
| Tru9I | TTAA | 6 cut(s) | 644, 696, 852, 1092, 1589, 2234 |
| TscAI | CASTG | 4 cut(s) | 775, 1299, 1465, 2152 |
| TseFI | GTSAC | 4 cut(s) | 320, 1787, 1964, 2028 |
| TseI | GCWGC | 4 cut(s) | 682, 826, 1733, 2162 |
| Tsp45I | GTSAC | 4 cut(s) | 320, 1787, 1964, 2028 |
| TspDTI | ATGAA | 7 cut(s) | 53, 624, 975, 1647, 2200, 2309, 2562 |
| TspGWI | ACGGA | 1 cut(s) | 650 |
| TspRI | CASTG | 4 cut(s) | 775, 1299, 1465, 2152 |
| Tth111I | GACNNNGTC | 3 cut(s) | 559, 1258, 2539 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 65, 263 |
| VpaK11BI | GGWCC | 1 cut(s) | 269 |
| VspI | ATTAAT | 1 cut(s) | 1589 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 550 |
| XapI | RAATTY | 4 cut(s) | 130, 911, 1532, 1576 |
| XbaI | TCTAGA | 2 cut(s) | 2095, 2543 |
| XceI | RCATGY | 5 cut(s) | 293, 508, 633, 672, 1901 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1061 |
| XhoI | CTCGAG | 1 cut(s) | 1773 |
| XmiI | GTMKAC | 1 cut(s) | 2182 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1239, 1638 |
| XspI | CTAG | 9 cut(s) | 707, 819, 830, 1857, 1868, 1955, 2096, 2481, 2544 |
| Zsp2I | ATGCAT | 1 cut(s) | 817 |