Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001229

Physical Location & Seq

Genomic Coordinates Reverse (-)

36063845 .. 36069712

Gene Structure 5868 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1267700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGCCTCCAGTGATGCTACCCTCTTTTCAAGTTCATTACCTGTTCTTCCACATCCAAAGTGTACTGAACGTGAATGGTACTGAGCAAGGCTGTCAATCTATTGATGATCTTTCGCCTGGCTTAAATAAACTCCACCAAAATTTGGAGGGTAACGATCCGCTTGAAGATATTGAAACTCATGCGATTGGAAGCTTGCTTCCTGGCGATGAGGAGGTGCTATTAGCTGGCATAGCAGATGATCTTGACCTCAGTGGGTTGCCTGGTTCACTGGAAGACTTGGAAGATTATGATCTCTTTGGTAGTGGAGGCGGCATGGAATTGGAAAGTGATGCACAGGAGAGCCTGAGAATTGGCATGTCAAAAGTAAGTTTAGCTGACGGTGCTACTGGGAACGGGATGGCTCAATTTGCCCTTCCAAATGGCGTGGGAGCTGTTGCTGGAGAACATCCATATGGAGAGCATCCTTCCAGAACATTGTTTGTGCGAAATATCAATAGTAATGTTGAGGATTCAGAACTGAGGACACTTTTTGAGCAATACGGGGATATTAGAACGCTGTACACTGCATGTAAACATAGGGGATTTGTGATGATATCATATTATGATATTCGTGCTGCTCGAACTGCTATGCGCACATTACAAAACAAGCCTCTGCGGCGGAGGAAGCTTGATATTCACTTCTCAATTCCAAAGGATAATCCATCAGAGAAGGATATTAATCAAGGAACTTTGGTAGTTTTCAATTTGGATCCATCAGTTTCAAATGAAGACCTCCGTCATATATTTGGGGCTTATGGCGAGGTGAAAGAGATAAGGGAAACACCACACAAGAGGCATCATAAATTTATTGAGTTTTATGATGTTAGAGCTGCAGAAGCAGCCCTTAAGTCATTAAATAGGAGTGACATAGCTGGTAAACGCATAAAGCTTGAACCCAGTCGACCTGGTGGAGCTCGTCGAAACTTGATGCTGCAACTGAATCAAGACCCTGAACAAGATGAATCTCAGAGTTATCGACATCCAGTGGGTTCCCCGATAACAATCTCTCCACCGGGTAACTGGGCACAGTTTAACAACCCAATGGAACATAGTCCTGTGCACAGTATTAGTAAATCTCCTGGTTTCGGGACTATGAGTCCAACAAACAGCAACCATTTGCCTGGATTAGCGTCAATTCTGCATCCCCAGATATCAAACAATGTGAAGGTTGCACCCATTGGCAATAACCATGGAAGGGGGAGTCATGTTGATCATATATTTTCCAGTACAAACTCAGACCATGGATCTGCATTTCAACAATCACACTCATTTCCAGAGAACTCAACCCATTTCCAAGTAGCCATGCCCTCGTTCAATCCTTCAACCTCAAGTGGATCTCATGTGGAAACTTTATCAGGCCCGCAATTTCTTTGGGGGAGTCCAACTCCTTATTCAGAAAACACCAACTCTTCAGCTTGGCATCGACAATCAGTGGGACATCAACTTACATCCAATGGGAAGGGCCATGCCTTTCCATTCTCAGGTCATAATGGCCCTTTGCTCAGCGCTTCCCATCATCACCATCATCATCATCATGTTGGATCTGCCCCATCTGGTGTTCCTTTAGACAGGCGCTTTAATTACTTCCCAGAGTCACCAGAAACATCTTTTTTGGGTCCTGCTGCCTATGGAGGCATGAGTTTGGGACCCAGTCGTGGAAATTTTATGTTTAACGTGGGTGCTCGTCCTTCTAGAAATGGTGGCATATCCTTACCAGGAAACATGTCTGAAGGTAGCTTTAAAATGGTGGGCTCACCGAAGCTTAGCCCTGTGTTTTTAGGTAATGGCCCATACGCAGGACTGCCACCTACAAGCATAGATGGTTTGATTGAGCGTGGGAGGAGCAGACGAATGGAGAATAATGGGACCCAGATTGATAGCAAGAAACAGTTTCAGCTTGATTTGGATAAGATTCTCTCCGGGGAAGATAATCGAACAACATTAATGATAAAAAATATTCCAAACAAGTACACCTCGAAAATGTTACTTGCTGCAATCGATGAAAATCACGGGGGTACTTTTGATTTTCTCTATCTGCCAATTGATTTTAAGAACAAATGCAATGTGGGCTATGCATTTATCAATATGCTGTCTCCTTCACACATCATCCCATTTTATGAGGCATTTAATGGAAAGAAGTGGGAGAAGTTCAATAGTGAGAAAGTTGCCTCCTTGGCTTATGCTCGAATCCAAGGGAAGGCGGCTCTTGTGGCCCACTTTCAAAACTCAAGCCTAATGAATGAAGACAAACGTTGTCGCCCAATACTCTTCCACTCAGAAGGTTCTGAGGCTGGTGATCAGATCATCCACGAACATCTTCCTTCCAACAATTTGAACATTCAGATCAGTCAGGAAAAAGAGTCGGACTCTGATTCGTCAGGAAGCCCAGACCGTGGCTTTGACGAGAAGCCAGATAAGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008150 GO:0010564 GO:0010638 GO:0033043 GO:0040008 GO:0040020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045787 GO:0045836 GO:0045927 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051445 GO:0051446 GO:0051726 GO:0051783 GO:0051785 GO:0065007 GO:0090068 GO:0097159 GO:1901363 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RRM_1 RRM_2

Protein Analysis

821

Amino Acids

90.08

Weight (kDa)

6.19

Isoelectric Point (pI)

54.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	159 - 223	4e-10	RNA recognition motif
RRM_5	PF13893	241 - 317	4.1e-06	RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
RRM_1	PF00076	244 - 309	2.3e-11	RNA recognition motif
RRM_2	PF04059	658 - 754	1e-53	RNA recognition motif 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29400 AT1G29400 AT1G29400
fragaria_vesca	FvH4_3g06390 FvH4_3g06390 FvH4_3g06390 FvH4_3g06390 FvH4_3g06390 FvH4_3g06390 FvH4_3g06390 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250 FvH4_6g38250
malus_domestica	MD05G1291700.v1.1 MD09G1151600.v1.1 MD10G1267700.v1.1 MD17G1139300.v1.1
prunus_persica	Prupe.3G172900_v2.0.a1 Prupe.4G073800_v2.0.a1
pyrus_communis	pycom05g26820 pycom09g07120 pycom10g22380 pycom17g13240
rosa_chinensis	RchiOBHm_Chr2g0151511 RchiOBHm_Chr5g0012911
rosa_laevigata	RLG00000020556 RLG00000031968
rosa_multiflora	Rmu_sc0000940.1_g000021 Rmu_sc0016318.1_g000001
rosa_roxburghii	Rroxscaffold_1G00063670 Rroxscaffold_2G00096390
rosa_rugosa	Rorug02G0426600 Rorug02G0426700 Rorug02G0426800 Rorug05G0005100 Rorug05G0005100 Rorug05G0005100
rosa_samantha	Rh2AG487500 Rh2BG499900 Rh2CG474000 Rh2DG511100 Rh5AG099400 Rh5BG096000 Rh5CG108300 Rh5DG094700
rosa_wichuraiana	Rw2G039960 Rw5G008680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	632
AccB7I	CCANNNNNTGG	2 cut(s)	142, 2432
AccI	GTMKAC	1 cut(s)	940
AciI	CCGC	6 cut(s)	158, 309, 655, 658, 1400, 2240
AclI	AACGTT	1 cut(s)	2290
AclWI	GGATC	6 cut(s)	149, 743, 756, 1291, 1381, 1588
AcsI	RAATTY	3 cut(s)	139, 842, 1699
AcuI	CTGAAG	2 cut(s)	1434, 1788
AfaI	GTAC	6 cut(s)	63, 79, 560, 1267, 2009, 2056
AfeI	AGCGCT	1 cut(s)	1546
AfiI	CCNNNNNNNGG	8 cut(s)	142, 1124, 1234, 1520, 1694, 1835, 2236, 2432
AflII	CTTAAG	1 cut(s)	884
AflIII	ACRYGT	1 cut(s)	1761
AhdI	GACNNNNNGTC	1 cut(s)	1134
AjnI	CCWGG	7 cut(s)	115, 199, 259, 943, 1117, 1159, 1753
AjuI	GAANNNNNNNTTGG	2 cut(s)	1414, 1446
Alw21I	GWGCWC	3 cut(s)	955, 1101, 1723
Alw26I	GTCTC	1 cut(s)	2136
Alw44I	GTGCAC	1 cut(s)	1097
AlwI	GGATC	6 cut(s)	149, 743, 756, 1291, 1381, 1588
Aor51HI	AGCGCT	1 cut(s)	1546
AoxI	GGCC	5 cut(s)	1396, 1501, 1531, 1825, 2250
ApaLI	GTGCAC	1 cut(s)	1097
ApeKI	GCWGC	6 cut(s)	614, 869, 878, 970, 1661, 2030
ApoI	RAATTY	3 cut(s)	139, 842, 1699
ArsI	GACNNNNNNTTYG	2 cut(s)	2420, 2452
AseI	ATTAAT	2 cut(s)	717, 1982
Asp700I	GAANNNNTTC	1 cut(s)	2355
AspLEI	GCGC	3 cut(s)	633, 1547, 1614
AspS9I	GGNCC	8 cut(s)	1397, 1501, 1532, 1655, 1685, 1826, 1905, 2251
AsuC2I	CCSGG	2 cut(s)	1053, 1960
AsuHPI	GGTGA	5 cut(s)	814, 1550, 1626, 1785, 2345
AvaII	GGWCC	3 cut(s)	1655, 1685, 1905
BaeGI	GKGCMC	2 cut(s)	1066, 1101
BamHI	GGATCC	1 cut(s)	748
BanII	GRGCYC	2 cut(s)	955, 1793
BbsI	GAAGAC	3 cut(s)	279, 774, 2289
Bbv12I	GWGCWC	3 cut(s)	955, 1101, 1723
BbvI	GCAGC	6 cut(s)	601, 856, 890, 957, 1648, 2017
BccI	CCATC	7 cut(s)	391, 709, 760, 1560, 1569, 1597, 1853
BciT130I	CCWGG	7 cut(s)	117, 201, 261, 945, 1119, 1161, 1755
BclI	TGATCA	2 cut(s)	1249, 2335
BcnI	CCSGG	2 cut(s)	1053, 1960
BcoDI	GTCTC	1 cut(s)	2136
BfaI	CTAG	1 cut(s)	1731
BfmI	CTRYAG	1 cut(s)	870
BfoI	RGCGCY	2 cut(s)	1548, 1615
BfrI	CTTAAG	1 cut(s)	884
BglI	GCCNNNNNGGC	2 cut(s)	655, 2213
BisI	GCNGC	9 cut(s)	310, 615, 656, 870, 879, 971, 1662, 2031, 2241
BlpI	GCTNAGC	2 cut(s)	1541, 1802
BlsI	GCNGC	9 cut(s)	311, 616, 657, 871, 880, 972, 1663, 2032, 2242
Bme1390I	CCNGG	9 cut(s)	117, 201, 261, 945, 1053, 1119, 1161, 1755, 1960
Bme18I	GGWCC	3 cut(s)	1655, 1685, 1905
BmeRI	GACNNNNNGTC	1 cut(s)	1134
BmgT120I	GGNCC	8 cut(s)	1397, 1501, 1532, 1655, 1685, 1826, 1905, 2251
BmiI	GGNNCC	7 cut(s)	750, 1030, 1656, 1686, 1687, 1906, 1907
BmrFI	CCNGG	9 cut(s)	117, 201, 261, 945, 1053, 1119, 1161, 1755, 1960
BmrI	ACTGGG	4 cut(s)	396, 930, 1069, 1683
BmsI	GCATC	7 cut(s)	3, 319, 469, 844, 957, 1189, 1468
BmuI	ACTGGG	4 cut(s)	396, 930, 1069, 1683
BoxI	GACNNNNGTC	1 cut(s)	774
BpiI	GAAGAC	3 cut(s)	279, 774, 2289
BplI	GAGNNNNNCTC	4 cut(s)	1408, 1440, 2390, 2422
BpmI	CTGGAG	1 cut(s)	459
Bpu1102I	GCTNAGC	2 cut(s)	1541, 1802
BpuEI	CTTGAG	2 cut(s)	1351, 2251
BpuMI	CCSGG	2 cut(s)	1053, 1960
Bsa29I	ATCGAT	1 cut(s)	2037
BsaBI	GATNNNNATC	4 cut(s)	288, 717, 1040, 2043
BsaJI	CCNNGG	6 cut(s)	1228, 1279, 1959, 2211, 2230, 2431
BsaXI	ACNNNNNCTCC	2 cut(s)	1030, 1060
Bsc4I	CCNNNNNNNGG	8 cut(s)	142, 1124, 1234, 1520, 1694, 1835, 2236, 2432
Bse1I	ACTGG	8 cut(s)	8, 273, 391, 936, 1022, 1064, 1263, 1689
Bse3DI	GCAATG	1 cut(s)	2107
Bse8I	GATNNNNATC	4 cut(s)	288, 717, 1040, 2043
BseBI	CCWGG	7 cut(s)	117, 201, 261, 945, 1119, 1161, 1755
BseCI	ATCGAT	1 cut(s)	2037
BseDI	CCNNGG	6 cut(s)	1228, 1279, 1959, 2211, 2230, 2431
BseGI	GGATG	9 cut(s)	51, 402, 445, 460, 1018, 1180, 1487, 2145, 2343
BseJI	GATNNNNATC	4 cut(s)	288, 717, 1040, 2043
BseLI	CCNNNNNNNGG	8 cut(s)	142, 1124, 1234, 1520, 1694, 1835, 2236, 2432
BseMI	GCAATG	1 cut(s)	2107
BseNI	ACTGG	8 cut(s)	8, 273, 391, 936, 1022, 1064, 1263, 1689
BseRI	GAGGAG	2 cut(s)	224, 1894
BseSI	GKGCMC	2 cut(s)	1066, 1101
BseXI	GCAGC	6 cut(s)	601, 856, 890, 957, 1648, 2017
BshFI	GGCC	5 cut(s)	1398, 1503, 1533, 1827, 2252
BshVI	ATCGAT	1 cut(s)	2037
BsiHKAI	GWGCWC	3 cut(s)	955, 1101, 1723
BsiSI	CCGG	2 cut(s)	1052, 1959
BslFI	GGGAC	4 cut(s)	1141, 1488, 1698, 1918
BslI	CCNNNNNNNGG	8 cut(s)	142, 1124, 1234, 1520, 1694, 1835, 2236, 2432
BsmAI	GTCTC	1 cut(s)	2136
BsmFI	GGGAC	4 cut(s)	1141, 1488, 1698, 1918
BsnI	GGCC	5 cut(s)	1398, 1503, 1533, 1827, 2252
Bsp1286I	GDGCHC	5 cut(s)	955, 1066, 1101, 1723, 1793
Bsp1407I	TGTACA	1 cut(s)	558
Bsp1720I	GCTNAGC	2 cut(s)	1541, 1802
Bsp19I	CCATGG	2 cut(s)	1228, 1279
BspACI	CCGC	6 cut(s)	158, 309, 655, 658, 1400, 2240
BspANI	GGCC	5 cut(s)	1398, 1503, 1533, 1827, 2252
BspDI	ATCGAT	1 cut(s)	2037
BspLI	GGNNCC	7 cut(s)	750, 1030, 1656, 1686, 1687, 1906, 1907
BspMAI	CTGCAG	1 cut(s)	874
BspPI	GGATC	6 cut(s)	149, 743, 756, 1291, 1381, 1588
BspTI	CTTAAG	1 cut(s)	884
BsrDI	GCAATG	1 cut(s)	2107
BsrGI	TGTACA	1 cut(s)	558
BsrI	ACTGG	8 cut(s)	8, 273, 391, 936, 1022, 1064, 1263, 1689
BssECI	CCNNGG	6 cut(s)	1228, 1279, 1959, 2211, 2230, 2431
BssT1I	CCWWGG	4 cut(s)	1228, 1279, 2211, 2230
Bst2UI	CCWGG	7 cut(s)	117, 201, 261, 945, 1119, 1161, 1755
Bst4CI	ACNGT	5 cut(s)	380, 1068, 1103, 1929, 2432
Bst6I	CTCTTC	2 cut(s)	1453, 2312
BstAFI	CTTAAG	1 cut(s)	884
BstAUI	TGTACA	1 cut(s)	558
BstC8I	GCNNGC	3 cut(s)	194, 226, 1400
BstDSI	CCRYGG	3 cut(s)	1228, 1279, 2431
BstF5I	GGATG	9 cut(s)	51, 402, 445, 460, 1018, 1180, 1487, 2145, 2343
BstH2I	RGCGCY	2 cut(s)	1548, 1615
BstHHI	GCGC	3 cut(s)	633, 1547, 1614
BstMAI	GTCTC	1 cut(s)	2136
BstNI	CCWGG	7 cut(s)	117, 201, 261, 945, 1119, 1161, 1755
BstNSI	RCATGY	3 cut(s)	358, 570, 1765
BstPAI	GACNNNNGTC	1 cut(s)	774
BstSCI	CCNGG	9 cut(s)	115, 199, 259, 943, 1051, 1117, 1159, 1753, 1958
BstSFI	CTRYAG	1 cut(s)	870
BstSLI	GKGCMC	2 cut(s)	1066, 1101
BstV1I	GCAGC	6 cut(s)	601, 856, 890, 957, 1648, 2017
BstV2I	GAAGAC	3 cut(s)	279, 774, 2289
BstX2I	RGATCY	4 cut(s)	748, 1283, 1373, 1580
BstXI	CCANNNNNNTGG	1 cut(s)	1160
BstYI	RGATCY	4 cut(s)	748, 1283, 1373, 1580
Bsu15I	ATCGAT	1 cut(s)	2037
BsuRI	GGCC	5 cut(s)	1398, 1503, 1533, 1827, 2252
BsuTUI	ATCGAT	1 cut(s)	2037
BtgI	CCRYGG	3 cut(s)	1228, 1279, 2431
BtgZI	GCGATG	1 cut(s)	219
BtsCI	GGATG	9 cut(s)	51, 402, 445, 460, 1018, 1180, 1487, 2145, 2343
BtsI	GCAGTG	1 cut(s)	561
BtsIMutI	CAGTG	6 cut(s)	15, 256, 266, 561, 1029, 1476
Cac8I	GCNNGC	3 cut(s)	194, 226, 1400
CfoI	GCGC	3 cut(s)	633, 1547, 1614
Cfr13I	GGNCC	8 cut(s)	1397, 1501, 1532, 1655, 1685, 1826, 1905, 2251
ClaI	ATCGAT	1 cut(s)	2037
CseI	GACGC	1 cut(s)	1158
CsiI	ACCWGGT	1 cut(s)	943
Csp6I	GTAC	6 cut(s)	62, 78, 559, 1266, 2008, 2055
CspCI	CAANNNNNGTGG	2 cut(s)	405, 440
CviQI	GTAC	6 cut(s)	62, 78, 559, 1266, 2008, 2055
DraI	TTTAAA	1 cut(s)	1780
DriI	GACNNNNNGTC	1 cut(s)	1134
Eam1104I	CTCTTC	2 cut(s)	1453, 2312
Eam1105I	GACNNNNNGTC	1 cut(s)	1134
EarI	CTCTTC	2 cut(s)	1453, 2312
EciI	GGCGGA	1 cut(s)	673
Ecl136II	GAGCTC	1 cut(s)	953
Eco130I	CCWWGG	4 cut(s)	1228, 1279, 2211, 2230
Eco24I	GRGCYC	2 cut(s)	955, 1793
Eco32I	GATATC	2 cut(s)	594, 1191
Eco47I	GGWCC	3 cut(s)	1655, 1685, 1905
Eco47III	AGCGCT	1 cut(s)	1546
Eco53kI	GAGCTC	1 cut(s)	953
Eco57I	CTGAAG	2 cut(s)	1434, 1788
EcoICRI	GAGCTC	1 cut(s)	953
EcoO109I	RGGNCCY	3 cut(s)	1655, 1685, 1905
EcoRII	CCWGG	7 cut(s)	115, 199, 259, 943, 1117, 1159, 1753
EcoRV	GATATC	2 cut(s)	594, 1191
EcoT14I	CCWWGG	4 cut(s)	1228, 1279, 2211, 2230
EcoT22I	ATGCAT	1 cut(s)	2116
EcoT38I	GRGCYC	2 cut(s)	955, 1793
ErhI	CCWWGG	4 cut(s)	1228, 1279, 2211, 2230
FalI	AAGNNNNNCTT	2 cut(s)	867, 899
FaqI	GGGAC	4 cut(s)	1141, 1488, 1698, 1918
FauI	CCCGC	1 cut(s)	1407
FauNDI	CATATG	1 cut(s)	451
FbaI	TGATCA	2 cut(s)	1249, 2335
FblI	GTMKAC	1 cut(s)	940
Fnu4HI	GCNGC	9 cut(s)	310, 615, 656, 870, 879, 971, 1662, 2031, 2241
FokI	GGATG	9 cut(s)	38, 409, 432, 447, 1005, 1167, 1474, 2132, 2330
FriOI	GRGCYC	2 cut(s)	955, 1793
Fsp4HI	GCNGC	9 cut(s)	310, 615, 656, 870, 879, 971, 1662, 2031, 2241
FspAI	RTGCGCAY	1 cut(s)	632
FspBI	CTAG	1 cut(s)	1731
FspI	TGCGCA	1 cut(s)	632
GlaI	GCGC	3 cut(s)	632, 1546, 1613
GluI	GCNGC	9 cut(s)	310, 615, 656, 870, 879, 971, 1662, 2031, 2241
GsuI	CTGGAG	1 cut(s)	459
HaeII	RGCGCY	2 cut(s)	1548, 1615
HaeIII	GGCC	5 cut(s)	1398, 1503, 1533, 1827, 2252
HapII	CCGG	2 cut(s)	1052, 1959
HgaI	GACGC	1 cut(s)	1158
HhaI	GCGC	3 cut(s)	633, 1547, 1614
Hin6I	GCGC	3 cut(s)	631, 1545, 1612
HinP1I	GCGC	3 cut(s)	631, 1545, 1612
HincII	GTYRAC	1 cut(s)	941
HindII	GTYRAC	1 cut(s)	941
HindIII	AAGCTT	4 cut(s)	190, 665, 926, 1799
HpaII	CCGG	2 cut(s)	1052, 1959
HphI	GGTGA	5 cut(s)	814, 1550, 1626, 1785, 2345
Hpy166II	GTNNAC	8 cut(s)	62, 266, 561, 572, 917, 941, 1099, 2010
Hpy188III	TCNNGA	8 cut(s)	242, 468, 983, 1126, 1313, 1731, 2390, 2418
Hpy8I	GTNNAC	8 cut(s)	62, 266, 561, 572, 917, 941, 1099, 2010
Hpy99I	CGWCG	1 cut(s)	960
HpyCH4III	ACNGT	5 cut(s)	380, 1068, 1103, 1929, 2432
HpyCH4IV	ACGT	3 cut(s)	69, 1713, 2290
HpySE526I	ACGT	3 cut(s)	69, 1713, 2290
HspAI	GCGC	3 cut(s)	631, 1545, 1612
KflI	GGGWCCC	2 cut(s)	1685, 1905
Ksp22I	TGATCA	2 cut(s)	1249, 2335
LmnI	GCTCC	3 cut(s)	428, 950, 1881
Lsp1109I	GCAGC	6 cut(s)	601, 856, 890, 957, 1648, 2017
LweI	GCATC	7 cut(s)	3, 319, 469, 844, 957, 1189, 1468
MabI	ACCWGGT	1 cut(s)	943
MaeI	CTAG	1 cut(s)	1731
MaeII	ACGT	3 cut(s)	69, 1713, 2290
MaeIII	GTNAC	5 cut(s)	149, 902, 1055, 1632, 2022
MfeI	CAATTG	1 cut(s)	2079
MflI	RGATCY	4 cut(s)	748, 1283, 1373, 1580
MhlI	GDGCHC	5 cut(s)	955, 1066, 1101, 1723, 1793
MlyI	GAGTC	6 cut(s)	1144, 1249, 1426, 1640, 2399, 2408
MmeI	TCCRAC	5 cut(s)	1163, 1445, 1558, 2382, 2388
Mph1103I	ATGCAT	1 cut(s)	2116
MroXI	GAANNNNTTC	1 cut(s)	2355
MslI	CAYNNNNRTG	2 cut(s)	450, 1572
MspCI	CTTAAG	1 cut(s)	884
MspI	CCGG	2 cut(s)	1052, 1959
MspR9I	CCNGG	9 cut(s)	117, 201, 261, 945, 1053, 1119, 1161, 1755, 1960
MunI	CAATTG	1 cut(s)	2079
MvaI	CCWGG	7 cut(s)	117, 201, 261, 945, 1119, 1161, 1755
NciI	CCSGG	2 cut(s)	1053, 1960
NcoI	CCATGG	2 cut(s)	1228, 1279
NdeI	CATATG	1 cut(s)	451
NlaIV	GGNNCC	7 cut(s)	750, 1030, 1656, 1686, 1687, 1906, 1907
NmuCI	GTSAC	2 cut(s)	902, 1632
NsbI	TGCGCA	1 cut(s)	632
NsiI	ATGCAT	1 cut(s)	2116
NspI	RCATGY	3 cut(s)	358, 570, 1765
PciI	ACATGT	1 cut(s)	1761
PcsI	WCGNNNNNNNCGW	1 cut(s)	616
PdmI	GAANNNNTTC	1 cut(s)	2355
PfeI	GAWTC	6 cut(s)	509, 979, 1001, 1951, 2226, 2411
PflFI	GACNNNGTC	1 cut(s)	1689
PflMI	CCANNNNNTGG	2 cut(s)	142, 2432
PkrI	GCNGC	9 cut(s)	311, 616, 657, 871, 880, 972, 1663, 2032, 2242
PleI	GAGTC	6 cut(s)	1143, 1248, 1425, 1639, 2399, 2407
PpsI	GAGTC	6 cut(s)	1143, 1248, 1425, 1639, 2399, 2407
PpuMI	RGGWCCY	3 cut(s)	1655, 1685, 1905
PscI	ACATGT	1 cut(s)	1761
PshAI	GACNNNNGTC	1 cut(s)	774
PshBI	ATTAAT	2 cut(s)	717, 1982
Psp124BI	GAGCTC	1 cut(s)	955
Psp1406I	AACGTT	1 cut(s)	2290
Psp5II	RGGWCCY	3 cut(s)	1655, 1685, 1905
Psp6I	CCWGG	7 cut(s)	115, 199, 259, 943, 1117, 1159, 1753
PspGI	CCWGG	7 cut(s)	115, 199, 259, 943, 1117, 1159, 1753
PspN4I	GGNNCC	7 cut(s)	750, 1030, 1656, 1686, 1687, 1906, 1907
PspPI	GGNCC	8 cut(s)	1397, 1501, 1532, 1655, 1685, 1826, 1905, 2251
PspPPI	RGGWCCY	3 cut(s)	1655, 1685, 1905
PstI	CTGCAG	1 cut(s)	874
PsuI	RGATCY	4 cut(s)	748, 1283, 1373, 1580
PsyI	GACNNNGTC	1 cut(s)	1689
RsaI	GTAC	6 cut(s)	63, 79, 560, 1267, 2009, 2056
RsaNI	GTAC	6 cut(s)	62, 78, 559, 1266, 2008, 2055
RseI	CAYNNNNRTG	2 cut(s)	450, 1572
SacI	GAGCTC	1 cut(s)	955
SalI	GTCGAC	1 cut(s)	939
SatI	GCNGC	9 cut(s)	310, 615, 656, 870, 879, 971, 1662, 2031, 2241
Sau96I	GGNCC	8 cut(s)	1397, 1501, 1532, 1655, 1685, 1826, 1905, 2251
SchI	GAGTC	6 cut(s)	1144, 1249, 1426, 1640, 2399, 2408
ScrFI	CCNGG	9 cut(s)	117, 201, 261, 945, 1053, 1119, 1161, 1755, 1960
SduI	GDGCHC	5 cut(s)	955, 1066, 1101, 1723, 1793
SexAI	ACCWGGT	1 cut(s)	943
SfaNI	GCATC	7 cut(s)	3, 319, 469, 844, 957, 1189, 1468
SfcI	CTRYAG	1 cut(s)	870
SinI	GGWCC	3 cut(s)	1655, 1685, 1905
SmiMI	CAYNNNNRTG	2 cut(s)	450, 1572
SmlI	CTYRAG	3 cut(s)	884, 1366, 2266
SmoI	CTYRAG	3 cut(s)	884, 1366, 2266
SsiI	CCGC	6 cut(s)	158, 309, 655, 658, 1400, 2240
SspI	AATATT	1 cut(s)	1996
SspMI	CTAG	1 cut(s)	1731
SstI	GAGCTC	1 cut(s)	955
StyD4I	CCNGG	9 cut(s)	115, 199, 259, 943, 1051, 1117, 1159, 1753, 1958
StyI	CCWWGG	4 cut(s)	1228, 1279, 2211, 2230
TaaI	ACNGT	5 cut(s)	380, 1068, 1103, 1929, 2432
TaiI	ACGT	3 cut(s)	72, 1716, 2293
TaqI	TCGA	9 cut(s)	619, 940, 958, 1015, 1462, 1972, 2015, 2037, 2224
TatI	WGTACW	4 cut(s)	61, 558, 1265, 2007
TauI	GCSGC	3 cut(s)	312, 658, 2243
TfiI	GAWTC	6 cut(s)	509, 979, 1001, 1951, 2226, 2411
TscAI	CASTG	6 cut(s)	15, 256, 273, 568, 1029, 1476
TseFI	GTSAC	2 cut(s)	902, 1632
TseI	GCWGC	6 cut(s)	614, 869, 878, 970, 1661, 2030
Tsp45I	GTSAC	2 cut(s)	902, 1632
TspDTI	ATGAA	6 cut(s)	23, 780, 1014, 2055, 2291, 2295
TspGWI	ACGGA	1 cut(s)	764
TspRI	CASTG	6 cut(s)	15, 256, 273, 568, 1029, 1476
Tth111I	GACNNNGTC	1 cut(s)	1689
Van91I	CCANNNNNTGG	2 cut(s)	142, 2432
Vha464I	CTTAAG	1 cut(s)	884
VneI	GTGCAC	1 cut(s)	1097
VpaK11BI	GGWCC	3 cut(s)	1655, 1685, 1905
VspI	ATTAAT	2 cut(s)	717, 1982
XapI	RAATTY	3 cut(s)	139, 842, 1699
XbaI	TCTAGA	1 cut(s)	1730
XceI	RCATGY	3 cut(s)	358, 570, 1765
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1056
XmiI	GTMKAC	1 cut(s)	940
XmnI	GAANNNNTTC	1 cut(s)	2355
XspI	CTAG	1 cut(s)	1731
Zsp2I	ATGCAT	1 cut(s)	2116

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.