Mei2-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1945 |
| Acc65I | GGTACC | 1 cut(s) | 1178 |
| AccB1I | GGYRCC | 1 cut(s) | 1178 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1666, 1754 |
| AccBSI | CCGCTC | 1 cut(s) | 548 |
| AccI | GTMKAC | 1 cut(s) | 1263 |
| AccII | CGCG | 1 cut(s) | 2055 |
| AciI | CCGC | 7 cut(s) | 432, 548, 1063, 1774, 1984, 2053, 2055 |
| AclI | AACGTT | 1 cut(s) | 2732 |
| AclWI | GGATC | 2 cut(s) | 928, 1678 |
| AcoI | YGGCCR | 1 cut(s) | 1562 |
| AcsI | RAATTY | 9 cut(s) | 190, 301, 965, 1471, 1477, 2046, 2359, 2650, 2746 |
| AcuI | CTGAAG | 2 cut(s) | 60, 810 |
| AfaI | GTAC | 6 cut(s) | 112, 1180, 1910, 2105, 2152, 2168 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 1243, 1244, 1666, 1754, 2413, 2704 |
| AhlI | ACTAGT | 1 cut(s) | 1439 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1522, 1554 |
| Alw21I | GWGCWC | 4 cut(s) | 161, 1015, 1078, 1640 |
| Alw26I | GTCTC | 6 cut(s) | 772, 949, 2232, 2417, 2523, 2708 |
| AlwI | GGATC | 2 cut(s) | 928, 1678 |
| AoxI | GGCC | 6 cut(s) | 45, 1562, 2393, 2416, 2684, 2707 |
| ApoI | RAATTY | 9 cut(s) | 190, 301, 965, 1471, 1477, 2046, 2359, 2650, 2746 |
| Asp718I | GGTACC | 1 cut(s) | 1178 |
| AspS9I | GGNCC | 7 cut(s) | 45, 594, 1448, 1505, 1934, 2416, 2707 |
| AsuC2I | CCSGG | 1 cut(s) | 2147 |
| AsuHPI | GGTGA | 6 cut(s) | 1146, 1475, 1716, 2135, 2732, 2801 |
| AvaII | GGWCC | 4 cut(s) | 594, 1448, 1505, 1934 |
| BaeGI | GKGCMC | 1 cut(s) | 1189 |
| BalI | TGGCCA | 1 cut(s) | 1564 |
| BanI | GGYRCC | 1 cut(s) | 1178 |
| BanII | GRGCYC | 4 cut(s) | 161, 1078, 1548, 1640 |
| BbsI | GAAGAC | 4 cut(s) | 339, 1545, 1829, 2067 |
| Bbv12I | GWGCWC | 4 cut(s) | 161, 1015, 1078, 1640 |
| BccI | CCATC | 8 cut(s) | 67, 394, 494, 1668, 1687, 1955, 2449, 2768 |
| BceAI | ACGGC | 1 cut(s) | 2780 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 145, 179 |
| BciVI | GTATCC | 2 cut(s) | 2333, 2624 |
| BclI | TGATCA | 1 cut(s) | 2082 |
| BcnI | CCSGG | 1 cut(s) | 2147 |
| BcoDI | GTCTC | 6 cut(s) | 772, 949, 2232, 2417, 2523, 2708 |
| BcuI | ACTAGT | 1 cut(s) | 1439 |
| BfaI | CTAG | 7 cut(s) | 75, 144, 384, 1440, 1626, 1869, 2447 |
| BfmI | CTRYAG | 4 cut(s) | 993, 1260, 1938, 2130 |
| BfuAI | ACCTGC | 1 cut(s) | 1945 |
| BfuI | GTATCC | 2 cut(s) | 2333, 2624 |
| BglII | AGATCT | 1 cut(s) | 644 |
| BlpI | GCTNAGC | 1 cut(s) | 488 |
| Bme18I | GGWCC | 4 cut(s) | 594, 1448, 1505, 1934 |
| BmgT120I | GGNCC | 7 cut(s) | 45, 594, 1448, 1505, 1934, 2416, 2707 |
| BmiI | GGNNCC | 3 cut(s) | 46, 1180, 1450 |
| BmsI | GCATC | 7 cut(s) | 111, 140, 592, 862, 884, 1080, 2449 |
| BpiI | GAAGAC | 4 cut(s) | 339, 1545, 1829, 2067 |
| BpmI | CTGGAG | 4 cut(s) | 413, 582, 1158, 2725 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1422 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 488 |
| BpuEI | CTTGAG | 2 cut(s) | 305, 796 |
| BpuMI | CCSGG | 1 cut(s) | 2147 |
| BsaAI | YACGTR | 2 cut(s) | 2321, 2612 |
| BsaBI | GATNNNNATC | 3 cut(s) | 366, 2139, 2826 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1153, 1183 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 1243, 1244, 1666, 1754, 2413, 2704 |
| Bse118I | RCCGGY | 3 cut(s) | 1629, 2391, 2682 |
| Bse1I | ACTGG | 5 cut(s) | 1936, 2313, 2431, 2604, 2722 |
| Bse3DI | GCAATG | 4 cut(s) | 161, 756, 2203, 2494 |
| Bse8I | GATNNNNATC | 3 cut(s) | 366, 2139, 2826 |
| BseGI | GGATG | 9 cut(s) | 102, 568, 583, 877, 1582, 1733, 2091, 2464, 2827 |
| BseJI | GATNNNNATC | 3 cut(s) | 366, 2139, 2826 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 1243, 1244, 1666, 1754, 2413, 2704 |
| BseMI | GCAATG | 4 cut(s) | 161, 756, 2203, 2494 |
| BseMII | CTCAG | 5 cut(s) | 108, 1244, 1413, 1523, 1660 |
| BseNI | ACTGG | 5 cut(s) | 1936, 2313, 2431, 2604, 2722 |
| BseRI | GAGGAG | 2 cut(s) | 410, 1161 |
| BseSI | GKGCMC | 1 cut(s) | 1189 |
| BseYI | CCCAGC | 2 cut(s) | 1034, 1058 |
| Bsh1236I | CGCG | 1 cut(s) | 2055 |
| BshFI | GGCC | 6 cut(s) | 47, 1564, 2395, 2418, 2686, 2709 |
| BshNI | GGYRCC | 1 cut(s) | 1178 |
| BsiHKAI | GWGCWC | 4 cut(s) | 161, 1015, 1078, 1640 |
| BsiSI | CCGG | 4 cut(s) | 1630, 2146, 2392, 2683 |
| BslFI | GGGAC | 4 cut(s) | 1219, 1434, 1593, 1620 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 1243, 1244, 1666, 1754, 2413, 2704 |
| BsmAI | GTCTC | 6 cut(s) | 772, 949, 2232, 2417, 2523, 2708 |
| BsmBI | CGTCTC | 1 cut(s) | 772 |
| BsmFI | GGGAC | 4 cut(s) | 1219, 1434, 1593, 1620 |
| BsmI | GAATGC | 2 cut(s) | 295, 1828 |
| BsnI | GGCC | 6 cut(s) | 47, 1564, 2395, 2418, 2686, 2709 |
| Bsp1286I | GDGCHC | 6 cut(s) | 161, 1015, 1078, 1189, 1548, 1640 |
| Bsp143I | GATC | 9 cut(s) | 367, 412, 644, 734, 933, 1670, 1966, 2082, 2443 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 488 |
| BspACI | CCGC | 7 cut(s) | 432, 548, 1063, 1774, 1984, 2053, 2055 |
| BspANI | GGCC | 6 cut(s) | 47, 1564, 2395, 2418, 2686, 2709 |
| BspCNI | CTCAG | 5 cut(s) | 109, 1245, 1414, 1522, 1659 |
| BspFNI | CGCG | 1 cut(s) | 2055 |
| BspHI | TCATGA | 1 cut(s) | 1740 |
| BspLI | GGNNCC | 3 cut(s) | 46, 1180, 1450 |
| BspMAI | CTGCAG | 2 cut(s) | 997, 1942 |
| BspMI | ACCTGC | 1 cut(s) | 1945 |
| BspPI | GGATC | 2 cut(s) | 928, 1678 |
| BspQI | GCTCTTC | 1 cut(s) | 540 |
| BspT107I | GGYRCC | 1 cut(s) | 1178 |
| BsrBI | CCGCTC | 1 cut(s) | 548 |
| BsrDI | GCAATG | 4 cut(s) | 161, 756, 2203, 2494 |
| BsrFI | RCCGGY | 3 cut(s) | 1629, 2391, 2682 |
| BsrI | ACTGG | 5 cut(s) | 1936, 2313, 2431, 2604, 2722 |
| BssAI | RCCGGY | 3 cut(s) | 1629, 2391, 2682 |
| BssMI | GATC | 9 cut(s) | 367, 412, 644, 734, 933, 1670, 1966, 2082, 2443 |
| BssT1I | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1912 |
| Bst4CI | ACNGT | 5 cut(s) | 342, 556, 2066, 2291, 2582 |
| Bst6I | CTCTTC | 4 cut(s) | 399, 540, 2408, 2699 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 128, 755 |
| BstBAI | YACGTR | 2 cut(s) | 2321, 2612 |
| BstDEI | CTNAG | 8 cut(s) | 117, 371, 488, 1100, 1253, 1422, 1509, 1646 |
| BstDSI | CCRYGG | 1 cut(s) | 2053 |
| BstF5I | GGATG | 9 cut(s) | 102, 568, 583, 877, 1582, 1733, 2091, 2464, 2827 |
| BstFNI | CGCG | 1 cut(s) | 2055 |
| BstKTI | GATC | 9 cut(s) | 370, 415, 647, 737, 936, 1673, 1969, 2085, 2446 |
| BstMAI | GTCTC | 6 cut(s) | 772, 949, 2232, 2417, 2523, 2708 |
| BstMBI | GATC | 9 cut(s) | 367, 412, 644, 734, 933, 1670, 1966, 2082, 2443 |
| BstSFI | CTRYAG | 4 cut(s) | 993, 1260, 1938, 2130 |
| BstSLI | GKGCMC | 1 cut(s) | 1189 |
| BstUI | CGCG | 1 cut(s) | 2055 |
| BstV2I | GAAGAC | 4 cut(s) | 339, 1545, 1829, 2067 |
| BstX2I | RGATCY | 3 cut(s) | 644, 933, 1670 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 1182, 2426, 2717 |
| BstYI | RGATCY | 3 cut(s) | 644, 933, 1670 |
| BsuI | GTATCC | 2 cut(s) | 2333, 2624 |
| BsuRI | GGCC | 6 cut(s) | 47, 1564, 2395, 2418, 2686, 2709 |
| BtgI | CCRYGG | 1 cut(s) | 2053 |
| BtsCI | GGATG | 9 cut(s) | 102, 568, 583, 877, 1582, 1733, 2091, 2464, 2827 |
| BtsI | GCAGTG | 2 cut(s) | 430, 744 |
| BtsIMutI | CAGTG | 9 cut(s) | 430, 744, 885, 1929, 1957, 2296, 2306, 2587, 2597 |
| BveI | ACCTGC | 1 cut(s) | 1945 |
| CciI | TCATGA | 1 cut(s) | 1740 |
| Cfr10I | RCCGGY | 3 cut(s) | 1629, 2391, 2682 |
| Cfr13I | GGNCC | 7 cut(s) | 45, 594, 1448, 1505, 1934, 2416, 2707 |
| Cfr42I | CCGCGG | 1 cut(s) | 2056 |
| CseI | GACGC | 1 cut(s) | 203 |
| Csp6I | GTAC | 6 cut(s) | 111, 1179, 1909, 2104, 2151, 2167 |
| CviQI | GTAC | 6 cut(s) | 111, 1179, 1909, 2104, 2151, 2167 |
| DdeI | CTNAG | 8 cut(s) | 117, 371, 488, 1100, 1253, 1422, 1509, 1646 |
| DpnI | GATC | 9 cut(s) | 369, 414, 646, 736, 935, 1672, 1968, 2084, 2445 |
| DpnII | GATC | 9 cut(s) | 367, 412, 644, 734, 933, 1670, 1966, 2082, 2443 |
| EaeI | YGGCCR | 1 cut(s) | 1562 |
| Eam1104I | CTCTTC | 4 cut(s) | 399, 540, 2408, 2699 |
| EarI | CTCTTC | 4 cut(s) | 399, 540, 2408, 2699 |
| EciI | GGCGGA | 1 cut(s) | 447 |
| Ecl136II | GAGCTC | 3 cut(s) | 159, 1076, 1638 |
| Eco130I | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1912 |
| Eco24I | GRGCYC | 4 cut(s) | 161, 1078, 1548, 1640 |
| Eco32I | GATATC | 4 cut(s) | 670, 717, 730, 838 |
| Eco47I | GGWCC | 4 cut(s) | 594, 1448, 1505, 1934 |
| Eco53kI | GAGCTC | 3 cut(s) | 159, 1076, 1638 |
| Eco57I | CTGAAG | 2 cut(s) | 60, 810 |
| EcoICRI | GAGCTC | 3 cut(s) | 159, 1076, 1638 |
| EcoO109I | RGGNCCY | 4 cut(s) | 45, 594, 1448, 1505 |
| EcoRI | GAATTC | 2 cut(s) | 2359, 2650 |
| EcoRV | GATATC | 4 cut(s) | 670, 717, 730, 838 |
| EcoT14I | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1912 |
| EcoT22I | ATGCAT | 3 cut(s) | 133, 877, 2762 |
| EcoT38I | GRGCYC | 4 cut(s) | 161, 1078, 1548, 1640 |
| ErhI | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1912 |
| Esp3I | CGTCTC | 1 cut(s) | 772 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 450, 482, 837, 869, 2325, 2357, 2616, 2648 |
| FaqI | GGGAC | 4 cut(s) | 1219, 1434, 1593, 1620 |
| FauI | CCCGC | 3 cut(s) | 541, 1977, 2048 |
| FauNDI | CATATG | 2 cut(s) | 1459, 2756 |
| FbaI | TGATCA | 1 cut(s) | 2082 |
| FblI | GTMKAC | 1 cut(s) | 1263 |
| FokI | GGATG | 9 cut(s) | 89, 555, 570, 884, 1569, 1720, 2078, 2471, 2834 |
| FriOI | GRGCYC | 4 cut(s) | 161, 1078, 1548, 1640 |
| FspBI | CTAG | 7 cut(s) | 75, 144, 384, 1440, 1626, 1869, 2447 |
| GsaI | CCCAGC | 2 cut(s) | 1038, 1062 |
| GsuI | CTGGAG | 4 cut(s) | 413, 582, 1158, 2725 |
| HaeIII | GGCC | 6 cut(s) | 47, 1564, 2395, 2418, 2686, 2709 |
| HapII | CCGG | 4 cut(s) | 1630, 2146, 2392, 2683 |
| HgaI | GACGC | 1 cut(s) | 203 |
| HincII | GTYRAC | 1 cut(s) | 256 |
| HindII | GTYRAC | 1 cut(s) | 256 |
| HindIII | AAGCTT | 1 cut(s) | 1049 |
| HpaII | CCGG | 4 cut(s) | 1630, 2146, 2392, 2683 |
| HphI | GGTGA | 6 cut(s) | 1146, 1475, 1716, 2135, 2732, 2801 |
| Hpy166II | GTNNAC | 7 cut(s) | 256, 1154, 1264, 1601, 1652, 1909, 2106 |
| Hpy188III | TCNNGA | 9 cut(s) | 323, 392, 1106, 1421, 1694, 1741, 2742, 2819, 2831 |
| Hpy8I | GTNNAC | 7 cut(s) | 256, 1154, 1264, 1601, 1652, 1909, 2106 |
| HpyCH4III | ACNGT | 5 cut(s) | 342, 556, 2066, 2291, 2582 |
| HpyCH4IV | ACGT | 5 cut(s) | 984, 1042, 2320, 2611, 2732 |
| HpyF3I | CTNAG | 8 cut(s) | 117, 371, 488, 1100, 1253, 1422, 1509, 1646 |
| HpySE526I | ACGT | 5 cut(s) | 984, 1042, 2320, 2611, 2732 |
| KpnI | GGTACC | 1 cut(s) | 1182 |
| KroI | GCCGGC | 3 cut(s) | 1629, 2391, 2682 |
| KroNI | GCCGGC | 3 cut(s) | 1631, 2393, 2684 |
| Ksp22I | TGATCA | 1 cut(s) | 2082 |
| KspI | CCGCGG | 1 cut(s) | 2056 |
| Kzo9I | GATC | 9 cut(s) | 367, 412, 644, 734, 933, 1670, 1966, 2082, 2443 |
| LguI | GCTCTTC | 1 cut(s) | 540 |
| LmnI | GCTCC | 8 cut(s) | 205, 545, 580, 1023, 1073, 1391, 1403, 1681 |
| LweI | GCATC | 7 cut(s) | 111, 140, 592, 862, 884, 1080, 2449 |
| MaeI | CTAG | 7 cut(s) | 75, 144, 384, 1440, 1626, 1869, 2447 |
| MaeII | ACGT | 5 cut(s) | 984, 1042, 2320, 2611, 2732 |
| MaeIII | GTNAC | 3 cut(s) | 2344, 2635, 2720 |
| MalI | GATC | 9 cut(s) | 369, 414, 646, 736, 935, 1672, 1968, 2084, 2445 |
| MbiI | CCGCTC | 1 cut(s) | 548 |
| MboI | GATC | 9 cut(s) | 367, 412, 644, 734, 933, 1670, 1966, 2082, 2443 |
| MflI | RGATCY | 3 cut(s) | 644, 933, 1670 |
| MhlI | GDGCHC | 6 cut(s) | 161, 1015, 1078, 1189, 1548, 1640 |
| MlsI | TGGCCA | 1 cut(s) | 1564 |
| MluNI | TGGCCA | 1 cut(s) | 1564 |
| MlyI | GAGTC | 6 cut(s) | 1264, 1378, 1475, 1987, 2341, 2632 |
| MmeI | TCCRAC | 2 cut(s) | 1162, 1325 |
| Mox20I | TGGCCA | 1 cut(s) | 1564 |
| Mph1103I | ATGCAT | 3 cut(s) | 133, 877, 2762 |
| MroNI | GCCGGC | 3 cut(s) | 1629, 2391, 2682 |
| MscI | TGGCCA | 1 cut(s) | 1564 |
| MslI | CAYNNNNRTG | 4 cut(s) | 573, 749, 2219, 2510 |
| Msp20I | TGGCCA | 1 cut(s) | 1564 |
| MspA1I | CMGCKG | 1 cut(s) | 2055 |
| MspI | CCGG | 4 cut(s) | 1630, 2146, 2392, 2683 |
| Mva1269I | GAATGC | 2 cut(s) | 295, 1828 |
| MvnI | CGCG | 1 cut(s) | 2055 |
| NaeI | GCCGGC | 3 cut(s) | 1631, 2393, 2684 |
| NciI | CCSGG | 1 cut(s) | 2147 |
| NdeI | CATATG | 2 cut(s) | 1459, 2756 |
| NdeII | GATC | 9 cut(s) | 367, 412, 644, 734, 933, 1670, 1966, 2082, 2443 |
| NgoMIV | GCCGGC | 3 cut(s) | 1629, 2391, 2682 |
| NlaIV | GGNNCC | 3 cut(s) | 46, 1180, 1450 |
| NmuCI | GTSAC | 3 cut(s) | 2344, 2635, 2720 |
| NsiI | ATGCAT | 3 cut(s) | 133, 877, 2762 |
| PagI | TCATGA | 1 cut(s) | 1740 |
| PciSI | GCTCTTC | 1 cut(s) | 540 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1135 |
| PctI | GAATGC | 2 cut(s) | 295, 1828 |
| PdiI | GCCGGC | 3 cut(s) | 1631, 2393, 2684 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1666, 1754 |
| PfoI | TCCNGGA | 1 cut(s) | 1840 |
| PleI | GAGTC | 6 cut(s) | 1263, 1377, 1474, 1986, 2341, 2632 |
| PpsI | GAGTC | 6 cut(s) | 1263, 1377, 1474, 1986, 2341, 2632 |
| Ppu21I | YACGTR | 2 cut(s) | 2321, 2612 |
| PpuMI | RGGWCCY | 3 cut(s) | 594, 1448, 1505 |
| Psp124BI | GAGCTC | 3 cut(s) | 161, 1078, 1640 |
| Psp1406I | AACGTT | 1 cut(s) | 2732 |
| Psp5II | RGGWCCY | 3 cut(s) | 594, 1448, 1505 |
| PspFI | CCCAGC | 2 cut(s) | 1034, 1058 |
| PspN4I | GGNNCC | 3 cut(s) | 46, 1180, 1450 |
| PspPI | GGNCC | 7 cut(s) | 45, 594, 1448, 1505, 1934, 2416, 2707 |
| PspPPI | RGGWCCY | 3 cut(s) | 594, 1448, 1505 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 667, 699 |
| PstI | CTGCAG | 2 cut(s) | 997, 1942 |
| PsuI | RGATCY | 3 cut(s) | 644, 933, 1670 |
| RsaI | GTAC | 6 cut(s) | 112, 1180, 1910, 2105, 2152, 2168 |
| RsaNI | GTAC | 6 cut(s) | 111, 1179, 1909, 2104, 2151, 2167 |
| RseI | CAYNNNNRTG | 4 cut(s) | 573, 749, 2219, 2510 |
| SacI | GAGCTC | 3 cut(s) | 161, 1078, 1640 |
| SacII | CCGCGG | 1 cut(s) | 2056 |
| SapI | GCTCTTC | 1 cut(s) | 540 |
| Sau3AI | GATC | 9 cut(s) | 367, 412, 644, 734, 933, 1670, 1966, 2082, 2443 |
| Sau96I | GGNCC | 7 cut(s) | 45, 594, 1448, 1505, 1934, 2416, 2707 |
| SchI | GAGTC | 6 cut(s) | 1264, 1378, 1475, 1987, 2341, 2632 |
| SduI | GDGCHC | 6 cut(s) | 161, 1015, 1078, 1189, 1548, 1640 |
| SfaNI | GCATC | 7 cut(s) | 111, 140, 592, 862, 884, 1080, 2449 |
| SfcI | CTRYAG | 4 cut(s) | 993, 1260, 1938, 2130 |
| Sfr303I | CCGCGG | 1 cut(s) | 2056 |
| SgrBI | CCGCGG | 1 cut(s) | 2056 |
| SinI | GGWCC | 4 cut(s) | 594, 1448, 1505, 1934 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 573, 749, 2219, 2510 |
| SmlI | CTYRAG | 2 cut(s) | 284, 775 |
| SmoI | CTYRAG | 2 cut(s) | 284, 775 |
| SpeI | ACTAGT | 1 cut(s) | 1439 |
| SsiI | CCGC | 7 cut(s) | 432, 548, 1063, 1774, 1984, 2053, 2055 |
| SspI | AATATT | 1 cut(s) | 70 |
| SspMI | CTAG | 7 cut(s) | 75, 144, 384, 1440, 1626, 1869, 2447 |
| SstI | GAGCTC | 3 cut(s) | 161, 1078, 1640 |
| StyI | CCWWGG | 5 cut(s) | 466, 813, 844, 1348, 1912 |
| TaaI | ACNGT | 5 cut(s) | 342, 556, 2066, 2291, 2582 |
| TaiI | ACGT | 5 cut(s) | 987, 1045, 2323, 2614, 2735 |
| TaqI | TCGA | 5 cut(s) | 155, 898, 1078, 1810, 2178 |
| TaqII | GACCGA | 1 cut(s) | 52 |
| TatI | WGTACW | 2 cut(s) | 2103, 2166 |
| TauI | GCSGC | 2 cut(s) | 1065, 1776 |
| TscAI | CASTG | 9 cut(s) | 430, 751, 885, 1936, 1957, 2296, 2313, 2587, 2604 |
| TseFI | GTSAC | 3 cut(s) | 2344, 2635, 2720 |
| Tsp45I | GTSAC | 3 cut(s) | 2344, 2635, 2720 |
| TspGWI | ACGGA | 2 cut(s) | 1378, 1575 |
| TspRI | CASTG | 9 cut(s) | 430, 751, 885, 1936, 1957, 2296, 2313, 2587, 2604 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1666, 1754 |
| VpaK11BI | GGWCC | 4 cut(s) | 594, 1448, 1505, 1934 |
| XapI | RAATTY | 9 cut(s) | 190, 301, 965, 1471, 1477, 2046, 2359, 2650, 2746 |
| XmiI | GTMKAC | 1 cut(s) | 1263 |
| XspI | CTAG | 7 cut(s) | 75, 144, 384, 1440, 1626, 1869, 2447 |
| Zsp2I | ATGCAT | 3 cut(s) | 133, 877, 2762 |