Rh7DG166400

Signal peptide peptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0006474
Physical Location & Seq
Reverse (-)
14898087 .. 14901895
3809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG166400.1

Sequence Viewer

Length: 1203 bp
ATGGTGTGTGAGCCAGATGAAACTGCTCTAGATATACACATACCTGCTATCATGCTCCCACAGGATGCTGGCGTGACCTTGGAGAAAATGTTAATGAGCAATTCAGTGGTGTCTGTGCAGTTGTACTCTCCACAACGGCCAGTGGTTGACATTGCGGAAGTATTTTTATGGCTGATGGCAGTTGGCACCATCTTGTGCGCATCTTATTGGTCTGCATGGAGTGCCAGAGAAGCTGCTATTGAACACGAAAAGTTGTTAAAGGATGCTGCTGATGAAATTCCAACTGTGAAAGCTCCTTTAGGTGCTGGTATCGTAGACATTACTACAACATCAGCAGTCTTGTTTGTCATTATTGCTTCATGCTTCTTGGTCATACTGTACAAGCTTATGTCAGCGTGGTTCGTTGAGCTTTTGGTTGTTCTTTTCTGCATAGGTGGTGTAGAGGGCTTGCAAACATGCTTGGTGTCTCTCTTGTCGAGGTGGTTTAAACGTACCGGAGAGGCGTACGTTAAATTACCTATCCTGGGAGCAATCTCATACCTAACTTTGGCTGTTTCTCCATTCTGCATAGCATTTGCTGTTCTCTGGGCAGTTTTTCGCACTGTTTCCTTTGCCTGGATCGGCCAAGATATTCTTGGAATTGCACTGATAATCACAGTTCTGCAAATTGTTCGTATACCCAATCTCAAGGTGGGTACGGTCCTCCTCAGTTGTGCCTTCTTGTATGACATATTTTGGGTGTTTGTTTCTAATAAGGTGTTCCATGAAAGTGTGATGATTGTGGTAGCTCGTGGTGACAGAAGTGGAGAGGATGGTATTCCAATGCTACTCAAGATCCCCCGCATGTTTGACCCATGGGGTGGTTACAGCATAATAGGATTTGGTGACATCCTGTTACCAGGCCTGCTTGTTGCATTCTCACTCAGGTATGATTGGTTGGCAAGCAAGAGTCTGCGAGCTGGTTACTTCTTGTGGGCAATGATTGCTTATGGATTAGGTCTTCTTATCACATATGTGGCATTAAACTTGATGGATGGGCATGGCCAACCAGCACTGCTTTACATTGTTCCATTTACACTAGGAACCTTACTGACATTAGGGAAGAAGAGAGGTGACTTACCGATTCTGTGGGGTAGAGGAGAGCCGGATAGGCTCTGCCCGCATGTTCGACTTGAACACAGTCGAGAACTGAGGGAAGAATAA

Protein Analysis

400

Amino Acids

44.24

Weight (kDa)

5.75

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_A22B PF04258 104 - 380 3e-102 Signal peptide peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 199
Acc36I ACCTGC 1 cut(s) 52
AccB1I GGYRCC 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 860
AccI GTMKAC 2 cut(s) 315, 676
AciI CCGC 3 cut(s) 155, 841, 1160
AclWI GGATC 2 cut(s) 626, 829
AcoI YGGCCR 3 cut(s) 137, 622, 1042
AcsI RAATTY 1 cut(s) 276
AfaI GTAC 5 cut(s) 125, 380, 493, 506, 697
AfiI CCNNNNNNNGG 4 cut(s) 524, 547, 615, 860
AgsI TTSAA 2 cut(s) 242, 1175
AjnI CCWGG 3 cut(s) 522, 614, 898
AleI CACNNNNGTG 1 cut(s) 1013
AluBI AGCT 6 cut(s) 233, 293, 385, 409, 788, 959
AluI AGCT 6 cut(s) 233, 293, 385, 409, 788, 959
Alw26I GTCTC 1 cut(s) 471
AlwI GGATC 2 cut(s) 626, 829
AoxI GGCC 4 cut(s) 137, 622, 901, 1042
ApeKI GCWGC 2 cut(s) 233, 266
ApoI RAATTY 1 cut(s) 276
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 700
AsuHPI GGTGA 3 cut(s) 806, 896, 1124
AvaII GGWCC 1 cut(s) 700
BalI TGGCCA 1 cut(s) 1044
BanI GGYRCC 1 cut(s) 185
BauI CACGAG 1 cut(s) 789
BbsI GAAGAC 1 cut(s) 992
BbvI GCAGC 2 cut(s) 220, 253
BccI CCATC 5 cut(s) 169, 197, 806, 1024, 1028
BceAI ACGGC 1 cut(s) 152
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 3 cut(s) 524, 616, 900
BcoDI GTCTC 1 cut(s) 471
BfaI CTAG 2 cut(s) 29, 1079
BfuAI ACCTGC 1 cut(s) 52
BglI GCCNNNNNGGC 1 cut(s) 1150
BisI GCNGC 2 cut(s) 234, 267
BlsI GCNGC 2 cut(s) 235, 268
Bme1390I CCNGG 3 cut(s) 524, 616, 900
Bme18I GGWCC 1 cut(s) 700
BmgT120I GGNCC 1 cut(s) 700
BmiI GGNNCC 2 cut(s) 187, 1084
BmrFI CCNGG 3 cut(s) 524, 616, 900
BmsI GCATC 3 cut(s) 55, 209, 253
BpiI GAAGAC 1 cut(s) 992
BpuEI CTTGAG 2 cut(s) 671, 815
BsaJI CCNNGG 3 cut(s) 78, 523, 854
BsaWI WCCGGW 1 cut(s) 494
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 4 cut(s) 524, 547, 615, 860
Bse1I ACTGG 1 cut(s) 140
Bse3DI GCAATG 2 cut(s) 150, 984
BseBI CCWGG 3 cut(s) 524, 616, 900
BseDI CCNNGG 3 cut(s) 78, 523, 854
BseGI GGATG 5 cut(s) 70, 268, 817, 888, 1039
BseLI CCNNNNNNNGG 4 cut(s) 524, 547, 615, 860
BseMI GCAATG 2 cut(s) 150, 984
BseMII CTCAG 3 cut(s) 721, 937, 1181
BseNI ACTGG 1 cut(s) 140
BseRI GAGGAG 2 cut(s) 695, 1152
BseXI GCAGC 2 cut(s) 220, 253
BsgI GTGCAG 1 cut(s) 137
BshFI GGCC 4 cut(s) 139, 624, 903, 1044
BshNI GGYRCC 1 cut(s) 185
BsiSI CCGG 2 cut(s) 495, 1145
BsiWI CGTACG 1 cut(s) 504
BslI CCNNNNNNNGG 4 cut(s) 524, 547, 615, 860
BsmAI GTCTC 1 cut(s) 471
BsmI GAATGC 1 cut(s) 914
BsnI GGCC 4 cut(s) 139, 624, 903, 1044
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 2 cut(s) 618, 834
Bsp19I CCATGG 1 cut(s) 854
BspACI CCGC 3 cut(s) 155, 841, 1160
BspANI GGCC 4 cut(s) 139, 624, 903, 1044
BspCNI CTCAG 3 cut(s) 720, 936, 1182
BspLI GGNNCC 2 cut(s) 187, 1084
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 2 cut(s) 626, 829
BspT107I GGYRCC 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 150, 984
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 1 cut(s) 140
BssECI CCNNGG 3 cut(s) 78, 523, 854
BssMI GATC 2 cut(s) 618, 834
BssNAI GTATAC 1 cut(s) 677
BssSI CACGAG 1 cut(s) 789
BssT1I CCWWGG 2 cut(s) 78, 854
Bst1107I GTATAC 1 cut(s) 677
Bst2BI CACGAG 1 cut(s) 789
Bst2UI CCWGG 3 cut(s) 524, 616, 900
Bst4CI ACNGT 6 cut(s) 286, 378, 604, 658, 700, 1181
Bst6I CTCTTC 1 cut(s) 1100
BstAPI GCANNNNNTGC 2 cut(s) 221, 983
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 6 cut(s) 70, 449, 905, 943, 957, 1160
BstDEI CTNAG 3 cut(s) 707, 923, 1190
BstDSI CCRYGG 1 cut(s) 854
BstF5I GGATG 5 cut(s) 70, 268, 817, 888, 1039
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 2 cut(s) 621, 837
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 2 cut(s) 618, 834
BstMWI GCNNNNNNNGC 5 cut(s) 221, 230, 983, 1150, 1159
BstNI CCWGG 3 cut(s) 524, 616, 900
BstNSI RCATGY 3 cut(s) 459, 847, 1166
BstSCI CCNGG 3 cut(s) 522, 614, 898
BstV1I GCAGC 2 cut(s) 220, 253
BstV2I GAAGAC 1 cut(s) 992
BstX2I RGATCY 1 cut(s) 834
BstYI RGATCY 1 cut(s) 834
BstZ17I GTATAC 1 cut(s) 677
BsuRI GGCC 4 cut(s) 139, 624, 903, 1044
BtgI CCRYGG 1 cut(s) 854
BtsCI GGATG 5 cut(s) 70, 268, 817, 888, 1039
BtsI GCAGTG 1 cut(s) 1052
BtsIMutI CAGTG 5 cut(s) 111, 147, 600, 644, 1052
BveI ACCTGC 1 cut(s) 52
Cac8I GCNNGC 6 cut(s) 70, 449, 905, 943, 957, 1160
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 700
Csp6I GTAC 5 cut(s) 124, 379, 492, 505, 696
CviAII CATG 9 cut(s) 52, 216, 360, 456, 764, 844, 855, 1040, 1163
CviQI GTAC 5 cut(s) 124, 379, 492, 505, 696
DdeI CTNAG 3 cut(s) 707, 923, 1190
DpnI GATC 2 cut(s) 620, 836
DpnII GATC 2 cut(s) 618, 834
DraI TTTAAA 1 cut(s) 487
EaeI YGGCCR 3 cut(s) 137, 622, 1042
Eam1104I CTCTTC 1 cut(s) 1100
EarI CTCTTC 1 cut(s) 1100
Eco130I CCWWGG 2 cut(s) 78, 854
Eco147I AGGCCT 1 cut(s) 903
Eco47I GGWCC 1 cut(s) 700
EcoRII CCWGG 3 cut(s) 522, 614, 898
EcoT14I CCWWGG 2 cut(s) 78, 854
ErhI CCWWGG 2 cut(s) 78, 854
FaeI CATG 9 cut(s) 55, 219, 363, 459, 767, 847, 858, 1043, 1166
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FatI CATG 9 cut(s) 51, 215, 359, 455, 763, 843, 854, 1039, 1162
FauI CCCGC 2 cut(s) 848, 1167
FauNDI CATATG 1 cut(s) 1012
FblI GTMKAC 2 cut(s) 315, 676
Fnu4HI GCNGC 2 cut(s) 234, 267
FokI GGATG 5 cut(s) 77, 275, 824, 875, 1046
Fsp4HI GCNGC 2 cut(s) 234, 267
FspAI RTGCGCAY 1 cut(s) 199
FspBI CTAG 2 cut(s) 29, 1079
FspI TGCGCA 1 cut(s) 199
GlaI GCGC 1 cut(s) 199
GluI GCNGC 2 cut(s) 234, 267
HaeIII GGCC 4 cut(s) 139, 624, 903, 1044
HapII CCGG 2 cut(s) 495, 1145
HhaI GCGC 1 cut(s) 200
Hin1II CATG 9 cut(s) 55, 219, 363, 459, 767, 847, 858, 1043, 1166
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 2 cut(s) 949, 1123
HpaII CCGG 2 cut(s) 495, 1145
HphI GGTGA 3 cut(s) 806, 896, 1124
Hpy166II GTNNAC 3 cut(s) 148, 316, 677
Hpy188III TCNNGA 3 cut(s) 29, 832, 1184
Hpy8I GTNNAC 3 cut(s) 148, 316, 677
HpyAV CCTTC 1 cut(s) 727
HpyCH4III ACNGT 6 cut(s) 286, 378, 604, 658, 700, 1181
HpyCH4IV ACGT 2 cut(s) 490, 507
HpyCH4V TGCA 8 cut(s) 118, 215, 429, 451, 567, 644, 664, 914
HpyF10VI GCNNNNNNNGC 5 cut(s) 221, 230, 983, 1150, 1159
HpyF3I CTNAG 3 cut(s) 707, 923, 1190
HpySE526I ACGT 2 cut(s) 490, 507
Hsp92II CATG 9 cut(s) 55, 219, 363, 459, 767, 847, 858, 1043, 1166
HspAI GCGC 1 cut(s) 198
Kzo9I GATC 2 cut(s) 618, 834
LmnI GCTCC 3 cut(s) 60, 298, 527
Lsp1109I GCAGC 2 cut(s) 220, 253
LweI GCATC 3 cut(s) 55, 209, 253
MaeI CTAG 2 cut(s) 29, 1079
MaeII ACGT 2 cut(s) 490, 507
MaeIII GTNAC 7 cut(s) 73, 794, 863, 884, 894, 962, 1112
MalI GATC 2 cut(s) 620, 836
MboI GATC 2 cut(s) 618, 834
MboII GAAGA 3 cut(s) 992, 1114, 1117
MflI RGATCY 1 cut(s) 834
MlsI TGGCCA 1 cut(s) 1044
MluCI AATT 5 cut(s) 100, 276, 512, 639, 666
MluNI TGGCCA 1 cut(s) 1044
MlyI GAGTC 1 cut(s) 958
MmeI TCCRAC 1 cut(s) 305
MnlI CCTC 9 cut(s) 436, 471, 493, 713, 716, 802, 1103, 1130, 1185
Mox20I TGGCCA 1 cut(s) 1044
MscI TGGCCA 1 cut(s) 1044
MseI TTAA 5 cut(s) 92, 257, 486, 510, 1022
MslI CAYNNNNRTG 2 cut(s) 768, 1013
Msp20I TGGCCA 1 cut(s) 1044
MspI CCGG 2 cut(s) 495, 1145
MspR9I CCNGG 3 cut(s) 524, 616, 900
MssI GTTTAAAC 1 cut(s) 487
Mva1269I GAATGC 1 cut(s) 914
MvaI CCWGG 3 cut(s) 524, 616, 900
MwoI GCNNNNNNNGC 5 cut(s) 221, 230, 983, 1150, 1159
NcoI CCATGG 1 cut(s) 854
NdeI CATATG 1 cut(s) 1012
NdeII GATC 2 cut(s) 618, 834
NlaIII CATG 9 cut(s) 55, 219, 363, 459, 767, 847, 858, 1043, 1166
NlaIV GGNNCC 2 cut(s) 187, 1084
NmuCI GTSAC 4 cut(s) 73, 794, 884, 1112
NsbI TGCGCA 1 cut(s) 199
NspI RCATGY 3 cut(s) 459, 847, 1166
OliI CACNNNNGTG 1 cut(s) 1013
PceI AGGCCT 1 cut(s) 903
PctI GAATGC 1 cut(s) 914
PfeI GAWTC 1 cut(s) 1123
Pfl23II CGTACG 1 cut(s) 504
PflMI CCANNNNNTGG 1 cut(s) 860
PkrI GCNGC 2 cut(s) 235, 268
PleI GAGTC 1 cut(s) 957
PmeI GTTTAAAC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 957
Psp6I CCWGG 3 cut(s) 522, 614, 898
PspGI CCWGG 3 cut(s) 522, 614, 898
PspLI CGTACG 1 cut(s) 504
PspN4I GGNNCC 2 cut(s) 187, 1084
PspPI GGNCC 1 cut(s) 700
PsuI RGATCY 1 cut(s) 834
RsaI GTAC 5 cut(s) 125, 380, 493, 506, 697
RsaNI GTAC 5 cut(s) 124, 379, 492, 505, 696
RseI CAYNNNNRTG 2 cut(s) 768, 1013
SaqAI TTAA 5 cut(s) 92, 257, 486, 510, 1022
SatI GCNGC 2 cut(s) 234, 267
Sau3AI GATC 2 cut(s) 618, 834
Sau96I GGNCC 1 cut(s) 700
SchI GAGTC 1 cut(s) 958
ScrFI CCNGG 3 cut(s) 524, 616, 900
SfaNI GCATC 3 cut(s) 55, 209, 253
SinI GGWCC 1 cut(s) 700
SmiMI CAYNNNNRTG 2 cut(s) 768, 1013
SmlI CTYRAG 2 cut(s) 686, 830
SmoI CTYRAG 2 cut(s) 686, 830
Sse9I AATT 5 cut(s) 100, 276, 512, 639, 666
SseBI AGGCCT 1 cut(s) 903
SsiI CCGC 3 cut(s) 155, 841, 1160
SspMI CTAG 2 cut(s) 29, 1079
StuI AGGCCT 1 cut(s) 903
StyD4I CCNGG 3 cut(s) 522, 614, 898
StyI CCWWGG 2 cut(s) 78, 854
TaaI ACNGT 6 cut(s) 286, 378, 604, 658, 700, 1181
TaiI ACGT 2 cut(s) 493, 510
TaqI TCGA 3 cut(s) 476, 1168, 1183
TasI AATT 5 cut(s) 100, 276, 512, 639, 666
TatI WGTACW 2 cut(s) 123, 378
TfiI GAWTC 1 cut(s) 1123
Tru1I TTAA 5 cut(s) 92, 257, 486, 510, 1022
Tru9I TTAA 5 cut(s) 92, 257, 486, 510, 1022
TscAI CASTG 5 cut(s) 111, 147, 607, 651, 1059
TseFI GTSAC 4 cut(s) 73, 794, 884, 1112
TseI GCWGC 2 cut(s) 233, 266
Tsp45I GTSAC 4 cut(s) 73, 794, 884, 1112
TspDTI ATGAA 4 cut(s) 33, 288, 348, 780
TspRI CASTG 5 cut(s) 111, 147, 607, 651, 1059
Van91I CCANNNNNTGG 1 cut(s) 860
VpaK11BI GGWCC 1 cut(s) 700
XapI RAATTY 1 cut(s) 276
XbaI TCTAGA 1 cut(s) 28
XceI RCATGY 3 cut(s) 459, 847, 1166
XcmI CCANNNNNNNNNTGG 2 cut(s) 632, 688
XmiI GTMKAC 2 cut(s) 315, 676
XspI CTAG 2 cut(s) 29, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.