Rorug07G0040000

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006369
Physical Location & Seq
Forward (+)
2819325 .. 2822296
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0040000.1

Sequence Viewer

Length: 1476 bp
ATGTCAGAAAAGAGTAGGATTCCGTCAAGATTCATTTCCTCCTTGATAACAGTTTCTGTGTTCCTTTTAGTTCTTTCTTCTGTGTTCCTTCTTCAATCTGGCAATACATCTTTCATTCCCAGATCAGTATTTGAGCTTATGCTTGTTAATGGCACATCAGTGTACTTTGAGCCTCATGTGAAAAAGGAACCATTTAAACTTCCTTTCCTCTCTTCTGAGGTTCCCCAAGTTGATGCTATAAAACCCTTAAAAAGAATAAGTGAATCAGCTTGTCAAGTTTCCGATTCGAGCAAGAAAGTGGGATCTGTAGAAAGAAAGAAAATGATGGTCTGTGACTCGAAGCAGGCTCTTTTGAGGGTATTTATGTATGAGTTGCCTCCTGAATTTCACTTTGGGTTATTGGGTTGGAAGGGAATGGAGAATCAAACATGGCCACAGCACATCCCACCTTACCCAGGCGGGCTGAATTTACAGCACAGTATAGAATATTGGCTCACCCTTGATCTTTTATCATCAGATAACCTAAATGTGGTCAGACCTTGTACAGTAGTCAGGGTGCACAATTCAAGTGAAGCTGATTTTATTTTTGTGCCATTCTTCTCATCTCTGAGTTACAACCGGCATTCTAAGCTTCATGAGAAGGAGAAAGTCAGTGTCAACAAGAGGTTGCAGGACAAGTTGGTGCAGTTTTTGATGGGCCGGAATGAATGGAAAAGAAAGGGTGGGAAGGATCATTTAATAGTTGCTCATCATCCAAATAGCATGTTGGATGCAAGAAAGAAATTGGGTTCTGCCATGTTCGTGCTTGCAGATTTTGGAAGATATCCTGCTGAAATAGCAAATCTTGGGAAGGATATAATCGCGCCTTACAGACATTTGGTGACGACTGTTCCCAGCAGCAAATCAGCCACATTCAAGGAGCGCCCTATACTAGTGTATTTCCGAGGAGCAATTTTCAGAAAAGATGGAGGAGTAATTCGCCAAGAATTGTATCATCTTCTCAAAGACGAGAAAGATGTAGACTTCACTTTTGGTAGCATTGGAGGAAATGGTGTTCGCAAGGCAACCCAAGGAATGGCCTCATCAAAGTTTTGCCTAAATATAGCTGGCGATACCCCATCCTCAAATCGCCTATTTGATGCCATTGCTAGTCATTGTGTTCCAGTAATAATTAGCGATGAGATTGAGCTACCAATTGAAGATGTCTTAGACTACTCACAATTTTGCATATTTGTTCGCGGGGCTGATGCTGTAAAGAAGGGTTACCTACTGAAACTTCTTCGAGGAATAAACAAAGACAAGTGGACCAAAATGTGGGAAAGGTTGAAGCAAGTTGTACATCATTTCGAATATCAATATCCCTCCCTGCCTGGTGATGCTGTTGATATGATTTGGCAGGCAGTTTCTCGCAAGATATCCTCTCTACAATTTAAATATCACAGGAAGAAGAGATACGACAGGTCTCAACTTGTTTAA

Protein Analysis

491

Amino Acids

56.02

Weight (kDa)

9.53

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 116 - 424 5.6e-59 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1075, 1314
AccI GTMKAC 1 cut(s) 1020
AccII CGCG 2 cut(s) 863, 1239
AciI CCGC 2 cut(s) 459, 1239
AclWI GGATC 2 cut(s) 310, 738
AcoI YGGCCR 1 cut(s) 431
AcsI RAATTY 2 cut(s) 383, 466
AfaI GTAC 3 cut(s) 164, 544, 1338
AfiI CCNNNNNNNGG 4 cut(s) 455, 529, 1075, 1314
AgsI TTSAA 5 cut(s) 95, 567, 916, 1199, 1327
AhlI ACTAGT 1 cut(s) 931
AjnI CCWGG 2 cut(s) 454, 1369
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AleI CACNNNNGTG 1 cut(s) 158
AloI GAACNNNNNNTCC 2 cut(s) 1038, 1070
AluBI AGCT 6 cut(s) 136, 269, 575, 631, 1106, 1189
AluI AGCT 6 cut(s) 136, 269, 575, 631, 1106, 1189
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 1 cut(s) 1467
Alw44I GTGCAC 1 cut(s) 557
AlwI GGATC 2 cut(s) 310, 738
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 3 cut(s) 431, 697, 1077
ApaLI GTGCAC 1 cut(s) 557
ApeKI GCWGC 1 cut(s) 897
ApoI RAATTY 2 cut(s) 383, 466
ArsI GACNNNNNNTTYG 2 cut(s) 1013, 1045
AspLEI GCGC 2 cut(s) 865, 924
AspS9I GGNCC 2 cut(s) 697, 1305
AsuHPI GGTGA 3 cut(s) 487, 892, 1385
AsuII TTCGAA 1 cut(s) 1347
AvaII GGWCC 1 cut(s) 1305
BaeGI GKGCMC 1 cut(s) 561
BalI TGGCCA 1 cut(s) 433
BarI GAAGNNNNNNTAC 2 cut(s) 1436, 1468
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 909
BccI CCATC 4 cut(s) 319, 688, 959, 1126
BciT130I CCWGG 2 cut(s) 456, 1371
BcoDI GTCTC 1 cut(s) 1467
BcuI ACTAGT 1 cut(s) 931
BfaI CTAG 2 cut(s) 932, 1149
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 925
BisI GCNGC 1 cut(s) 898
BlsI GCNGC 1 cut(s) 899
Bme1390I CCNGG 2 cut(s) 456, 1371
Bme18I GGWCC 1 cut(s) 1305
BmgT120I GGNCC 2 cut(s) 697, 1305
BmiI GGNNCC 2 cut(s) 189, 222
BmrFI CCNGG 2 cut(s) 456, 1371
BmsI GCATC 5 cut(s) 223, 760, 1129, 1237, 1366
Bpu14I TTCGAA 1 cut(s) 1347
BsaI GGTCTC 1 cut(s) 1467
BsaJI CCNNGG 3 cut(s) 454, 943, 1069
Bsc4I CCNNNNNNNGG 4 cut(s) 455, 529, 1075, 1314
Bse118I RCCGGY 1 cut(s) 618
Bse1I ACTGG 1 cut(s) 1163
Bse3DI GCAATG 1 cut(s) 1143
BseBI CCWGG 2 cut(s) 456, 1371
BseDI CCNNGG 3 cut(s) 454, 943, 1069
BseGI GGATG 4 cut(s) 441, 751, 775, 1118
BseLI CCNNNNNNNGG 4 cut(s) 455, 529, 1075, 1314
BseMI GCAATG 1 cut(s) 1143
BseMII CTCAG 2 cut(s) 207, 599
BseNI ACTGG 1 cut(s) 1163
BseRI GAGGAG 2 cut(s) 960, 984
BseSI GKGCMC 1 cut(s) 561
BseXI GCAGC 1 cut(s) 909
BseYI CCCAGC 1 cut(s) 893
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 2 cut(s) 863, 1239
BshFI GGCC 3 cut(s) 433, 699, 1079
BsiHKAI GWGCWC 1 cut(s) 561
BsiSI CCGG 2 cut(s) 619, 700
BslI CCNNNNNNNGG 4 cut(s) 455, 529, 1075, 1314
BsmAI GTCTC 1 cut(s) 1467
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 3 cut(s) 433, 699, 1079
Bso31I GGTCTC 1 cut(s) 1467
Bsp119I TTCGAA 1 cut(s) 1347
Bsp1286I GDGCHC 1 cut(s) 561
Bsp1407I TGTACA 2 cut(s) 542, 1336
Bsp143I GATC 4 cut(s) 122, 302, 502, 730
BspACI CCGC 2 cut(s) 459, 1239
BspANI GGCC 3 cut(s) 433, 699, 1079
BspCNI CTCAG 2 cut(s) 208, 600
BspFNI CGCG 2 cut(s) 863, 1239
BspHI TCATGA 1 cut(s) 634
BspLI GGNNCC 2 cut(s) 189, 222
BspPI GGATC 2 cut(s) 310, 738
BspT104I TTCGAA 1 cut(s) 1347
BspTNI GGTCTC 1 cut(s) 1467
BsrDI GCAATG 1 cut(s) 1143
BsrFI RCCGGY 1 cut(s) 618
BsrGI TGTACA 2 cut(s) 542, 1336
BsrI ACTGG 1 cut(s) 1163
BssAI RCCGGY 1 cut(s) 618
BssECI CCNNGG 3 cut(s) 454, 943, 1069
BssMI GATC 4 cut(s) 122, 302, 502, 730
BssT1I CCWWGG 1 cut(s) 1069
Bst2UI CCWGG 2 cut(s) 456, 1371
Bst4CI ACNGT 4 cut(s) 52, 479, 547, 889
Bst6I CTCTTC 2 cut(s) 217, 1442
BstAUI TGTACA 2 cut(s) 542, 1336
BstBI TTCGAA 1 cut(s) 1347
BstC8I GCNNGC 5 cut(s) 345, 461, 807, 1108, 1398
BstDEI CTNAG 4 cut(s) 216, 608, 627, 1207
BstEII GGTNACC 1 cut(s) 1262
BstENI CCTNNNNNAGG 1 cut(s) 453
BstF5I GGATG 4 cut(s) 441, 751, 775, 1118
BstFNI CGCG 2 cut(s) 863, 1239
BstH2I RGCGCY 1 cut(s) 925
BstHHI GCGC 2 cut(s) 865, 924
BstKTI GATC 4 cut(s) 125, 305, 505, 733
BstMAI GTCTC 1 cut(s) 1467
BstMBI GATC 4 cut(s) 122, 302, 502, 730
BstMWI GCNNNNNNNGC 2 cut(s) 628, 836
BstNI CCWGG 2 cut(s) 456, 1371
BstNSI RCATGY 1 cut(s) 766
BstPI GGTNACC 1 cut(s) 1262
BstSCI CCNGG 2 cut(s) 454, 1369
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 561
BstUI CGCG 2 cut(s) 863, 1239
BstV1I GCAGC 1 cut(s) 909
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BsuRI GGCC 3 cut(s) 433, 699, 1079
BtgZI GCGATG 1 cut(s) 1191
BtsCI GGATG 4 cut(s) 441, 751, 775, 1118
BtsIMutI CAGTG 2 cut(s) 165, 658
Cac8I GCNNGC 5 cut(s) 345, 461, 807, 1108, 1398
CaiI CAGNNNCTG 1 cut(s) 56
CciI TCATGA 1 cut(s) 634
CfoI GCGC 2 cut(s) 865, 924
Cfr10I RCCGGY 1 cut(s) 618
Cfr13I GGNCC 2 cut(s) 697, 1305
Csp6I GTAC 3 cut(s) 163, 543, 1337
CviAII CATG 5 cut(s) 176, 429, 635, 763, 796
CviQI GTAC 3 cut(s) 163, 543, 1337
DdeI CTNAG 4 cut(s) 216, 608, 627, 1207
DpnI GATC 4 cut(s) 124, 304, 504, 732
DpnII GATC 4 cut(s) 122, 302, 502, 730
DraI TTTAAA 2 cut(s) 196, 1432
EaeI YGGCCR 1 cut(s) 431
Eam1104I CTCTTC 2 cut(s) 217, 1442
EarI CTCTTC 2 cut(s) 217, 1442
Eco130I CCWWGG 1 cut(s) 1069
Eco31I GGTCTC 1 cut(s) 1467
Eco32I GATATC 2 cut(s) 824, 1416
Eco47I GGWCC 1 cut(s) 1305
Eco91I GGTNACC 1 cut(s) 1262
EcoNI CCTNNNNNAGG 1 cut(s) 453
EcoO65I GGTNACC 1 cut(s) 1262
EcoRII CCWGG 2 cut(s) 454, 1369
EcoRV GATATC 2 cut(s) 824, 1416
EcoT14I CCWWGG 1 cut(s) 1069
ErhI CCWWGG 1 cut(s) 1069
FaeI CATG 5 cut(s) 179, 432, 638, 766, 799
FatI CATG 5 cut(s) 175, 428, 634, 762, 795
FauI CCCGC 2 cut(s) 452, 1232
FblI GTMKAC 1 cut(s) 1020
Fnu4HI GCNGC 1 cut(s) 898
FokI GGATG 4 cut(s) 428, 738, 782, 1105
Fsp4HI GCNGC 1 cut(s) 898
FspBI CTAG 2 cut(s) 932, 1149
GlaI GCGC 2 cut(s) 864, 923
GluI GCNGC 1 cut(s) 898
GsaI CCCAGC 1 cut(s) 897
HaeII RGCGCY 1 cut(s) 925
HaeIII GGCC 3 cut(s) 433, 699, 1079
HapII CCGG 2 cut(s) 619, 700
HhaI GCGC 2 cut(s) 865, 924
Hin1II CATG 5 cut(s) 179, 432, 638, 766, 799
Hin6I GCGC 2 cut(s) 863, 922
HinP1I GCGC 2 cut(s) 863, 922
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 6 cut(s) 19, 30, 263, 284, 335, 421
HpaII CCGG 2 cut(s) 619, 700
HphI GGTGA 3 cut(s) 487, 892, 1385
Hpy166II GTNNAC 5 cut(s) 163, 559, 658, 1021, 1305
Hpy188I TCNGA 8 cut(s) 7, 217, 283, 517, 536, 609, 944, 959
Hpy188III TCNNGA 3 cut(s) 27, 380, 635
Hpy8I GTNNAC 5 cut(s) 163, 559, 658, 1021, 1305
HpyAV CCTTC 6 cut(s) 98, 403, 634, 721, 844, 1252
HpyCH4III ACNGT 4 cut(s) 52, 479, 547, 889
HpyCH4V TGCA 6 cut(s) 559, 670, 685, 773, 809, 1227
HpyF10VI GCNNNNNNNGC 2 cut(s) 628, 836
HpyF3I CTNAG 4 cut(s) 216, 608, 627, 1207
Hsp92II CATG 5 cut(s) 179, 432, 638, 766, 799
HspAI GCGC 2 cut(s) 863, 922
Kzo9I GATC 4 cut(s) 122, 302, 502, 730
LmnI GCTCC 2 cut(s) 919, 947
Lsp1109I GCAGC 1 cut(s) 909
LweI GCATC 5 cut(s) 223, 760, 1129, 1237, 1366
MaeI CTAG 2 cut(s) 932, 1149
MaeIII GTNAC 4 cut(s) 332, 611, 880, 1262
MalI GATC 4 cut(s) 124, 304, 504, 732
MboI GATC 4 cut(s) 122, 302, 502, 730
MfeI CAATTG 1 cut(s) 1194
MflI RGATCY 1 cut(s) 302
MhlI GDGCHC 1 cut(s) 561
MlsI TGGCCA 1 cut(s) 433
MluNI TGGCCA 1 cut(s) 433
MlyI GAGTC 1 cut(s) 329
MmeI TCCRAC 2 cut(s) 386, 747
Mox20I TGGCCA 1 cut(s) 433
MscI TGGCCA 1 cut(s) 433
MseI TTAA 6 cut(s) 147, 195, 248, 737, 1431, 1474
MslI CAYNNNNRTG 2 cut(s) 158, 800
Msp20I TGGCCA 1 cut(s) 433
MspI CCGG 2 cut(s) 619, 700
MspR9I CCNGG 2 cut(s) 456, 1371
MunI CAATTG 1 cut(s) 1194
Mva1269I GAATGC 1 cut(s) 622
MvaI CCWGG 2 cut(s) 456, 1371
MvnI CGCG 2 cut(s) 863, 1239
MwoI GCNNNNNNNGC 2 cut(s) 628, 836
NdeII GATC 4 cut(s) 122, 302, 502, 730
NlaIII CATG 5 cut(s) 179, 432, 638, 766, 799
NlaIV GGNNCC 2 cut(s) 189, 222
NmuCI GTSAC 2 cut(s) 332, 880
NspI RCATGY 1 cut(s) 766
NspV TTCGAA 1 cut(s) 1347
OliI CACNNNNGTG 1 cut(s) 158
PagI TCATGA 1 cut(s) 634
PctI GAATGC 1 cut(s) 622
PfeI GAWTC 5 cut(s) 19, 30, 263, 284, 421
PflMI CCANNNNNTGG 2 cut(s) 1075, 1314
PkrI GCNGC 1 cut(s) 899
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
Psp6I CCWGG 2 cut(s) 454, 1369
PspEI GGTNACC 1 cut(s) 1262
PspFI CCCAGC 1 cut(s) 893
PspGI CCWGG 2 cut(s) 454, 1369
PspN4I GGNNCC 2 cut(s) 189, 222
PspPI GGNCC 2 cut(s) 697, 1305
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 302
RsaI GTAC 3 cut(s) 164, 544, 1338
RsaNI GTAC 3 cut(s) 163, 543, 1337
RseI CAYNNNNRTG 2 cut(s) 158, 800
SaqAI TTAA 6 cut(s) 147, 195, 248, 737, 1431, 1474
SatI GCNGC 1 cut(s) 898
Sau3AI GATC 4 cut(s) 122, 302, 502, 730
Sau96I GGNCC 2 cut(s) 697, 1305
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 456, 1371
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 5 cut(s) 223, 760, 1129, 1237, 1366
SfcI CTRYAG 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 1347
SinI GGWCC 1 cut(s) 1305
SmiI ATTTAAAT 1 cut(s) 1432
SmiMI CAYNNNNRTG 2 cut(s) 158, 800
SpeI ACTAGT 1 cut(s) 931
SsiI CCGC 2 cut(s) 459, 1239
SspI AATATT 1 cut(s) 488
SspMI CTAG 2 cut(s) 932, 1149
StyD4I CCNGG 2 cut(s) 454, 1369
StyI CCWWGG 1 cut(s) 1069
SwaI ATTTAAAT 1 cut(s) 1432
TaaI ACNGT 4 cut(s) 52, 479, 547, 889
TaqI TCGA 4 cut(s) 287, 338, 1282, 1347
TatI WGTACW 3 cut(s) 162, 542, 1336
TfiI GAWTC 5 cut(s) 19, 30, 263, 284, 421
Tru1I TTAA 6 cut(s) 147, 195, 248, 737, 1431, 1474
Tru9I TTAA 6 cut(s) 147, 195, 248, 737, 1431, 1474
TscAI CASTG 2 cut(s) 165, 658
TseFI GTSAC 2 cut(s) 332, 880
TseI GCWGC 1 cut(s) 897
Tsp45I GTSAC 2 cut(s) 332, 880
TspDTI ATGAA 4 cut(s) 22, 103, 623, 720
TspGWI ACGGA 1 cut(s) 12
TspRI CASTG 2 cut(s) 165, 658
Van91I CCANNNNNTGG 2 cut(s) 1075, 1314
VneI GTGCAC 1 cut(s) 557
VpaK11BI GGWCC 1 cut(s) 1305
XagI CCTNNNNNAGG 1 cut(s) 453
XapI RAATTY 2 cut(s) 383, 466
XceI RCATGY 1 cut(s) 766
XmiI GTMKAC 1 cut(s) 1020
XspI CTAG 2 cut(s) 932, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.