Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006369

Physical Location & Seq

Genomic Coordinates Forward (+)

20888683 .. 20901260

Gene Structure 12578 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1085100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2304 bp

ATGGCCTTCACTTTCCTCTCAATCCCCAACTTTCTCTCTCCTCAACCTCACTTTCTCTCTCTAAGATCCAATTTCACTTCCCTCAAACCCTCAATTCCCTCTCTTTCCACCCGGTTCATTAAGCCCACCCACGCAATCGGGCCGGACGGCAAGTACTACCCGGACCCGGCCGACGACGACCCGCCTGAAGCCCCGGAAGACTCGGGCCACGGAGTGTCCAAGTTCCAGCAAATCCAGCGGCAGGCCTCGCGCCACCGCAAGCTCCAAGAGGAAGACTACTTAAAGCACCAGGACACCTTCCTCAACGCCATTGCTGACGTGGAGGACGCGCCGGAGAATCCCAATTCCGTAATCAACGACAACTCCGGGGACGATTTGTTCGGCGACATCGACCAGGCGATTGCCTTGAAACGCAAGGAGTTTGTGAAGCAGGGGCTTCTGAAGCCAAACCCTAAGAAAGAGATTGTGGCGGTTGACGAATTGGACCCTGAGGAGGTAGTGGATTTGGAGGAGATTGAGGAGCTTCAGGGGATTAGGGTTGTTGGCGAGGACGAAGAGGAAGACGGGCCGGAGAAATTCGATCCCAAGGTCAGTGATTTGGATGGTAAAGATGGGATTTTGCGTTTGAATTCGGAGTTTGATTTGGATTTTGATAATTATGGTAAAACTAGGGCTAGGATTGTGGAACCGAAGTTTAAAATGGGCTTGGCTGAGCTTTTGGATGAGAGTAAAGTGGTGCCCGTGTCGGTTTATGGTGACTTAGAAGTTGAGATTACAGGAATTCAACACGATTCGAGGGTGGTTAATGCCGGTGATTTGTTTGTGTGCTGTGTTGGGAGCAAGACCGACGGGCATTTGTATTTGAGTGAGGCTATTAAGAGAGGTGCGGTGGCTGTTGTAGCTAGTAAAGAGATATACATGGAGGAAAGTCTGGGGTGTAAGGCATTGGTCATTGTGGAAGATACAAATGCGGATTTACCTGCATTGGCTGCGTCGTTTTATCGGTACCCGTCGAAGAATATGGCAGTGATTGGGATAACGGGAACAAATGGGAAGACCACAACTGCATATTTGATAAAAGGGATGTATGAAGCGATGGGATTGAGGACCGGTATGTTGAGTACGGTTGCATATTATGTGCATGGGGATAATAAGTTGGAGTCACCTAACACGACCCCGGATGCTGTTTTGGTTCAAAATTTGATGGCTAAGATGCTCCATAATGGGGCTGAAGCCGTTGTCATGGAGGCTTCTTCTCATGGTCTTGCTCTATCCAGGTGCGATGAAGTTGACTTTGACATAGCAGTTTTCACAAATTTAACGAGAGACCATTTAGATTTTCACAAGACTGAGGAGGAGTATAGGGATGCAAAGGCTAAGTTGTTTTCAAGGATGGTGGATCCGGAACGGCACAGGAAAGTTGTGAACATTGATGATCCGAATTCAACCTTCTTTATTGCACAAGGCAACCCAGATGTTCCAGTTGTGACCTTTGCCATGGAGAATAAGAATGCAGACGTTCACCCCTTGAAGTTTGAACTTTCCTTGTTTGAGACGCAGGTTTTAGTCAATACTCCAAATGGTATATTGGAGATATCATCAGGCTTACTTGGAAGACATAATATATACAATATACTGGCTTCTGTGGCTGTTGGGATTGCAGTCGGATCACCATTGGAGGATATCGTTAGAGGAATTGAAGAGGTTGATGCAGTTCCAGGGAGGTGTGAATTAATAGATGAGGAACAGGCCTTTGGAGTAATTGTGGACTATGCTCATACTCCTGATGCCTTGTCTAGGCTATTGGATTCTGTAAGGGAGCTTGGTTCAAGGAGGATTATTACTGTAATTGGTTGTCCTGGAGAGAGTGACAGAGGGAAAAGACCCATGATGACGAAGATTGCAACAGATAAAAGTGACGTGACCATACTGACGTCTGACAATCCAAAGAATGAAGATCCATTGGACATTTTGGATGATATGTTGGCTGGTGTAGGATGGACAATGCAAGATTACTTAAAACACGGGGAGAACGATTACTACCCACCTCTTGCAAATGGTCATAGGCTTTTCCTTCATGATATTAGACGAGTAGCCGTACGTTGTGCTGTTGCCATGGGCGAGGAAGGTGATATGGTTGTGGTTGCTGGCAAAGGGCATGAAGCATATCAGATCGAAGGTGGCAAAAAAGAGTTCTTTGATGACCGGGAAGAGTGCCGAGAGGCGTTACAGTACGTTGATGAGCTTCACCAAGCTGGAATAGACACAAGTGAATTTCCATGGAGGTTACCTGAAAGTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009295 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0010468 GO:0016043 GO:0019222 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044424 GO:0044444 GO:0044464 GO:0048285 GO:0050789 GO:0060255 GO:0065007 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

768

Amino Acids

84.97

Weight (kDa)

4.72

Isoelectric Point (pI)

37.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mur_ligase	PF01225	257 - 320	6.4e-08	Mur ligase family, catalytic domain
Mur_ligase_M	PF08245	346 - 551	1e-57	Mur ligase middle domain
Mur_ligase_C	PF02875	574 - 719	6.2e-25	Mur ligase, glutamate ligase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006369)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63680 AT1G63680 AT1G63680
fragaria_vesca	FvH4_5g02610 FvH4_5g02610 FvH4_5g02610 FvH4_5g02610
malus_domestica	MD06G1085100.v1.1 MD14G1106100.v1.1 MD14G1106300.v1.1
prunus_persica	Prupe.5G101100_v2.0.a1
pyrus_communis	pycom06g08120
rosa_chinensis	RchiOBHm_Chr7g0197491
rosa_laevigata	RLG00000003936 RLG00000003970
rosa_multiflora	Rmu_co8124254.1_g000001 Rmu_co8184758.1_g000001 Rmu_sc0002167.1_g000003 Rmu_sc0013361.1_g000005
rosa_roxburghii	Rroxscaffold_3G00258420
rosa_rugosa	Rorug07G0040000 Rorug07G0040100
rosa_samantha	Rh7AG165200 Rh7BG167300 Rh7CG173700 Rh7DG166600
rosa_wichuraiana	Rw7G014400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1937
Acc36I	ACCTGC	2 cut(s)	988, 1549
Acc65I	GGTACC	1 cut(s)	1005
AccB1I	GGYRCC	2 cut(s)	736, 1005
AccII	CGCG	2 cut(s)	250, 329
AccIII	TCCGGA	1 cut(s)	1402
AciI	CCGC	6 cut(s)	182, 238, 256, 470, 887, 971
AclWI	GGATC	7 cut(s)	60, 575, 1394, 1407, 1430, 1675, 1952
AcoI	YGGCCR	1 cut(s)	168
AcsI	RAATTY	7 cut(s)	575, 628, 780, 1198, 1315, 1441, 2273
AcuI	CTGAAG	4 cut(s)	207, 461, 509, 1251
AcyI	GRCGYC	1 cut(s)	1934
AdeI	CACNNNGTG	1 cut(s)	214
AfaI	GTAC	5 cut(s)	155, 1007, 1123, 2100, 2234
AfiI	CCNNNNNNNGG	5 cut(s)	166, 241, 493, 1225, 1797
AgeI	ACCGGT	1 cut(s)	1109
AjiI	CACGTC	2 cut(s)	319, 1921
AjnI	CCWGG	5 cut(s)	288, 393, 1274, 1717, 1858
AjuI	GAANNNNNNNTTGG	2 cut(s)	1737, 1769
AloI	GAACNNNNNNTCC	2 cut(s)	362, 394
AluBI	AGCT	7 cut(s)	262, 523, 715, 902, 1822, 2245, 2255
AluI	AGCT	7 cut(s)	262, 523, 715, 902, 1822, 2245, 2255
Alw26I	GTCTC	2 cut(s)	1320, 1547
AlwI	GGATC	7 cut(s)	60, 575, 1394, 1407, 1430, 1675, 1952
Ama87I	CYCGRG	1 cut(s)	202
Aor13HI	TCCGGA	1 cut(s)	1402
AoxI	GGCC	7 cut(s)	3, 140, 168, 205, 243, 566, 1749
ApeKI	GCWGC	1 cut(s)	989
ApoI	RAATTY	7 cut(s)	575, 628, 780, 1198, 1315, 1441, 2273
ArsI	GACNNNNNNTTYG	2 cut(s)	362, 394
AseI	ATTAAT	1 cut(s)	1733
AsiGI	ACCGGT	1 cut(s)	1109
Asp718I	GGTACC	1 cut(s)	1005
AspLEI	GCGC	2 cut(s)	252, 331
AspS9I	GGNCC	6 cut(s)	140, 163, 205, 484, 566, 1107
AsuC2I	CCSGG	7 cut(s)	112, 161, 167, 194, 367, 1178, 2207
AsuHPI	GGTGA	7 cut(s)	767, 824, 1155, 1514, 1662, 2141, 2240
AvaI	CYCGRG	1 cut(s)	202
AvaII	GGWCC	3 cut(s)	163, 484, 1107
AxyI	CCTNAGG	1 cut(s)	489
BaeGI	GKGCMC	1 cut(s)	741
BamHI	GGATCC	1 cut(s)	1399
BanI	GGYRCC	2 cut(s)	736, 1005
BbsI	GAAGAC	5 cut(s)	204, 279, 567, 1061, 1621
BbvI	GCAGC	1 cut(s)	976
BccI	CCATC	6 cut(s)	596, 605, 1090, 1198, 1387, 1992
BceAI	ACGGC	4 cut(s)	163, 1220, 1424, 2081
BciT130I	CCWGG	5 cut(s)	290, 395, 1276, 1719, 1860
BcnI	CCSGG	7 cut(s)	112, 161, 167, 194, 367, 1178, 2207
BcoDI	GTCTC	2 cut(s)	1320, 1547
BfaI	CTAG	4 cut(s)	669, 675, 903, 1797
BfuAI	ACCTGC	2 cut(s)	988, 1549
BisI	GCNGC	2 cut(s)	239, 990
BlpI	GCTNAGC	1 cut(s)	711
BlsI	GCNGC	2 cut(s)	240, 991
BmcAI	AGTACT	1 cut(s)	155
Bme18I	GGWCC	3 cut(s)	163, 484, 1107
BmeT110I	CYCGRG	1 cut(s)	202
BmgBI	CACGTC	2 cut(s)	319, 1921
BmgT120I	GGNCC	6 cut(s)	140, 163, 205, 484, 566, 1107
BmiI	GGNNCC	6 cut(s)	165, 486, 687, 738, 1007, 1401
BmsI	GCATC	5 cut(s)	1171, 1203, 1357, 1699, 1777
BpiI	GAAGAC	5 cut(s)	204, 279, 567, 1061, 1621
BpmI	CTGGAG	1 cut(s)	1881
Bpu1102I	GCTNAGC	1 cut(s)	711
BpuMI	CCSGG	7 cut(s)	112, 161, 167, 194, 367, 1178, 2207
BsaHI	GRCGYC	1 cut(s)	1934
BsaI	GGTCTC	1 cut(s)	1320
BsaJI	CCNNGG	9 cut(s)	192, 208, 366, 585, 1176, 1497, 1718, 2115, 2279
BsaWI	WCCGGW	2 cut(s)	1109, 1402
BsaXI	ACNNNNNCTCC	4 cut(s)	347, 377, 1749, 1779
Bsc4I	CCNNNNNNNGG	5 cut(s)	166, 241, 493, 1225, 1797
Bse118I	RCCGGY	2 cut(s)	809, 1109
Bse1I	ACTGG	2 cut(s)	1481, 1641
Bse21I	CCTNAGG	1 cut(s)	489
Bse3DI	GCAATG	1 cut(s)	309
BseAI	TCCGGA	1 cut(s)	1402
BseBI	CCWGG	5 cut(s)	290, 395, 1276, 1719, 1860
BseDI	CCNNGG	9 cut(s)	192, 208, 366, 585, 1176, 1497, 1718, 2115, 2279
BseGI	GGATG	8 cut(s)	607, 727, 1089, 1186, 1372, 1398, 1981, 2003
BseLI	CCNNNNNNNGG	5 cut(s)	166, 241, 493, 1225, 1797
BseMI	GCAATG	1 cut(s)	309
BseMII	CTCAG	3 cut(s)	480, 702, 1341
BseNI	ACTGG	2 cut(s)	1481, 1641
BseRI	GAGGAG	6 cut(s)	30, 506, 524, 533, 1367, 1370
BseSI	GKGCMC	1 cut(s)	741
BseX3I	CGGCCG	1 cut(s)	168
BseXI	GCAGC	1 cut(s)	976
Bsh1236I	CGCG	2 cut(s)	250, 329
Bsh1285I	CGRYCG	1 cut(s)	171
BshFI	GGCC	7 cut(s)	5, 142, 170, 207, 245, 568, 1751
BshNI	GGYRCC	2 cut(s)	736, 1005
BshTI	ACCGGT	1 cut(s)	1109
BsiEI	CGRYCG	1 cut(s)	171
BsiHKCI	CYCGRG	1 cut(s)	202
BsiWI	CGTACG	1 cut(s)	2098
BslFI	GGGAC	1 cut(s)	383
BslI	CCNNNNNNNGG	5 cut(s)	166, 241, 493, 1225, 1797
BsmAI	GTCTC	2 cut(s)	1320, 1547
BsmBI	CGTCTC	1 cut(s)	1547
BsmFI	GGGAC	1 cut(s)	383
BsmI	GAATGC	1 cut(s)	1516
BsnI	GGCC	7 cut(s)	5, 142, 170, 207, 245, 568, 1751
Bso31I	GGTCTC	1 cut(s)	1320
BsoBI	CYCGRG	1 cut(s)	202
Bsp1286I	GDGCHC	1 cut(s)	741
Bsp13I	TCCGGA	1 cut(s)	1402
Bsp143I	GATC	7 cut(s)	65, 580, 1399, 1435, 1667, 1957, 2172
Bsp1720I	GCTNAGC	1 cut(s)	711
Bsp19I	CCATGG	3 cut(s)	1497, 2115, 2279
BspACI	CCGC	6 cut(s)	182, 238, 256, 470, 887, 971
BspANI	GGCC	7 cut(s)	5, 142, 170, 207, 245, 568, 1751
BspCNI	CTCAG	3 cut(s)	481, 703, 1342
BspEI	TCCGGA	1 cut(s)	1402
BspFNI	CGCG	2 cut(s)	250, 329
BspHI	TCATGA	1 cut(s)	2077
BspLI	GGNNCC	6 cut(s)	165, 486, 687, 738, 1007, 1401
BspMI	ACCTGC	2 cut(s)	988, 1549
BspPI	GGATC	7 cut(s)	60, 575, 1394, 1407, 1430, 1675, 1952
BspT107I	GGYRCC	2 cut(s)	736, 1005
BspTNI	GGTCTC	1 cut(s)	1320
BsrDI	GCAATG	1 cut(s)	309
BsrFI	RCCGGY	2 cut(s)	809, 1109
BsrI	ACTGG	2 cut(s)	1481, 1641
BssAI	RCCGGY	2 cut(s)	809, 1109
BssECI	CCNNGG	9 cut(s)	192, 208, 366, 585, 1176, 1497, 1718, 2115, 2279
BssMI	GATC	7 cut(s)	65, 580, 1399, 1435, 1667, 1957, 2172
BssNI	GRCGYC	1 cut(s)	1934
BssT1I	CCWWGG	4 cut(s)	585, 1497, 2115, 2279
Bst2UI	CCWGG	5 cut(s)	290, 395, 1276, 1719, 1860
Bst4CI	ACNGT	3 cut(s)	1126, 1846, 2232
Bst6I	CTCTTC	3 cut(s)	549, 1695, 2205
BstACI	GRCGYC	1 cut(s)	1934
BstAPI	GCANNNNNTGC	1 cut(s)	989
BstC8I	GCNNGC	3 cut(s)	243, 260, 2149
BstDEI	CTNAG	8 cut(s)	62, 453, 489, 711, 760, 1209, 1350, 1377
BstDSI	CCRYGG	4 cut(s)	208, 1497, 2115, 2279
BstEII	GGTNACC	1 cut(s)	2286
BstENI	CCTNNNNNAGG	1 cut(s)	1795
BstF5I	GGATG	8 cut(s)	607, 727, 1089, 1186, 1372, 1398, 1981, 2003
BstFNI	CGCG	2 cut(s)	250, 329
BstHHI	GCGC	2 cut(s)	252, 331
BstKTI	GATC	7 cut(s)	68, 583, 1402, 1438, 1670, 1960, 2175
BstMAI	GTCTC	2 cut(s)	1320, 1547
BstMBI	GATC	7 cut(s)	65, 580, 1399, 1435, 1667, 1957, 2172
BstMCI	CGRYCG	1 cut(s)	171
BstMWI	GCNNNNNNNGC	6 cut(s)	235, 247, 442, 899, 989, 1646
BstNI	CCWGG	5 cut(s)	290, 395, 1276, 1719, 1860
BstPI	GGTNACC	1 cut(s)	2286
BstSLI	GKGCMC	1 cut(s)	741
BstUI	CGCG	2 cut(s)	250, 329
BstV1I	GCAGC	1 cut(s)	976
BstV2I	GAAGAC	5 cut(s)	204, 279, 567, 1061, 1621
BstX2I	RGATCY	3 cut(s)	65, 1399, 1957
BstYI	RGATCY	3 cut(s)	65, 1399, 1957
BstZI	CGGCCG	1 cut(s)	168
Bsu36I	CCTNAGG	1 cut(s)	489
BsuRI	GGCC	7 cut(s)	5, 142, 170, 207, 245, 568, 1751
BtgI	CCRYGG	4 cut(s)	208, 1497, 2115, 2279
BtgZI	GCGATG	2 cut(s)	1109, 1296
BtrI	CACGTC	2 cut(s)	319, 1921
BtsCI	GGATG	8 cut(s)	607, 727, 1089, 1186, 1372, 1398, 1981, 2003
BtsI	GCAGTG	1 cut(s)	1032
BtsIMutI	CAGTG	2 cut(s)	598, 1032
BveI	ACCTGC	2 cut(s)	988, 1549
Cac8I	GCNNGC	3 cut(s)	243, 260, 2149
CciI	TCATGA	1 cut(s)	2077
CfoI	GCGC	2 cut(s)	252, 331
Cfr10I	RCCGGY	2 cut(s)	809, 1109
Cfr13I	GGNCC	6 cut(s)	140, 163, 205, 484, 566, 1107
CseI	GACGC	3 cut(s)	335, 981, 1564
Csp6I	GTAC	5 cut(s)	154, 1006, 1122, 2099, 2233
CspAI	ACCGGT	1 cut(s)	1109
CspCI	CAANNNNNGTGG	2 cut(s)	1377, 1412
CviQI	GTAC	5 cut(s)	154, 1006, 1122, 2099, 2233
DdeI	CTNAG	8 cut(s)	62, 453, 489, 711, 760, 1209, 1350, 1377
DpnI	GATC	7 cut(s)	67, 582, 1401, 1437, 1669, 1959, 2174
DpnII	GATC	7 cut(s)	65, 580, 1399, 1435, 1667, 1957, 2172
DraI	TTTAAA	1 cut(s)	697
DraIII	CACNNNGTG	1 cut(s)	214
EaeI	YGGCCR	1 cut(s)	168
EagI	CGGCCG	1 cut(s)	168
Eam1104I	CTCTTC	3 cut(s)	549, 1695, 2205
EarI	CTCTTC	3 cut(s)	549, 1695, 2205
EclXI	CGGCCG	1 cut(s)	168
Eco130I	CCWWGG	4 cut(s)	585, 1497, 2115, 2279
Eco147I	AGGCCT	2 cut(s)	245, 1751
Eco31I	GGTCTC	1 cut(s)	1320
Eco32I	GATATC	2 cut(s)	1596, 1684
Eco47I	GGWCC	3 cut(s)	163, 484, 1107
Eco52I	CGGCCG	1 cut(s)	168
Eco57I	CTGAAG	4 cut(s)	207, 461, 509, 1251
Eco81I	CCTNAGG	1 cut(s)	489
Eco88I	CYCGRG	1 cut(s)	202
Eco91I	GGTNACC	1 cut(s)	2286
EcoNI	CCTNNNNNAGG	1 cut(s)	1795
EcoO65I	GGTNACC	1 cut(s)	2286
EcoRI	GAATTC	3 cut(s)	628, 780, 1441
EcoRII	CCWGG	5 cut(s)	288, 393, 1274, 1717, 1858
EcoRV	GATATC	2 cut(s)	1596, 1684
EcoT14I	CCWWGG	4 cut(s)	585, 1497, 2115, 2279
ErhI	CCWWGG	4 cut(s)	585, 1497, 2115, 2279
Esp3I	CGTCTC	1 cut(s)	1547
FaqI	GGGAC	1 cut(s)	383
FauI	CCCGC	1 cut(s)	189
Fnu4HI	GCNGC	2 cut(s)	239, 990
FokI	GGATG	8 cut(s)	614, 734, 1096, 1193, 1379, 1405, 1988, 2010
Fsp4HI	GCNGC	2 cut(s)	239, 990
FspBI	CTAG	4 cut(s)	669, 675, 903, 1797
GlaI	GCGC	2 cut(s)	251, 330
GluI	GCNGC	2 cut(s)	239, 990
GsuI	CTGGAG	1 cut(s)	1881
HaeIII	GGCC	7 cut(s)	5, 142, 170, 207, 245, 568, 1751
HgaI	GACGC	3 cut(s)	335, 981, 1564
HhaI	GCGC	2 cut(s)	252, 331
Hin1I	GRCGYC	1 cut(s)	1934
Hin6I	GCGC	2 cut(s)	250, 329
HinP1I	GCGC	2 cut(s)	250, 329
HincII	GTYRAC	2 cut(s)	475, 1291
HindII	GTYRAC	2 cut(s)	475, 1291
HinfI	GANTC	5 cut(s)	200, 337, 791, 1160, 1808
HphI	GGTGA	7 cut(s)	767, 824, 1155, 1514, 1662, 2141, 2240
Hpy166II	GTNNAC	5 cut(s)	475, 1291, 1426, 1522, 1768
Hpy188I	TCNGA	6 cut(s)	441, 634, 1440, 1667, 1939, 2172
Hpy188III	TCNNGA	3 cut(s)	1403, 1784, 2078
Hpy8I	GTNNAC	5 cut(s)	475, 1291, 1426, 1522, 1768
Hpy99I	CGWCG	5 cut(s)	176, 179, 851, 997, 1015
HpyAV	CCTTC	6 cut(s)	16, 307, 1459, 2084, 2120, 2171
HpyCH4III	ACNGT	3 cut(s)	1126, 1846, 2232
HpyCH4IV	ACGT	6 cut(s)	318, 1518, 1920, 1934, 2101, 2235
HpyF10VI	GCNNNNNNNGC	6 cut(s)	235, 247, 442, 899, 989, 1646
HpyF3I	CTNAG	8 cut(s)	62, 453, 489, 711, 760, 1209, 1350, 1377
HpySE526I	ACGT	6 cut(s)	318, 1518, 1920, 1934, 2101, 2235
Hsp92I	GRCGYC	1 cut(s)	1934
HspAI	GCGC	2 cut(s)	250, 329
Kpn2I	TCCGGA	1 cut(s)	1402
KpnI	GGTACC	1 cut(s)	1009
Kzo9I	GATC	7 cut(s)	65, 580, 1399, 1435, 1667, 1957, 2172
LmnI	GCTCC	5 cut(s)	267, 520, 837, 1221, 1819
Lsp1109I	GCAGC	1 cut(s)	976
LweI	GCATC	5 cut(s)	1171, 1203, 1357, 1699, 1777
MaeI	CTAG	4 cut(s)	669, 675, 903, 1797
MaeII	ACGT	6 cut(s)	318, 1518, 1920, 1934, 2101, 2235
MaeIII	GTNAC	8 cut(s)	755, 1161, 1486, 1868, 1916, 1921, 2226, 2286
MalI	GATC	7 cut(s)	67, 582, 1401, 1437, 1669, 1959, 2174
MboI	GATC	7 cut(s)	65, 580, 1399, 1435, 1667, 1957, 2172
MflI	RGATCY	3 cut(s)	65, 1399, 1957
MhlI	GDGCHC	1 cut(s)	741
MlyI	GAGTC	2 cut(s)	194, 1169
MmeI	TCCRAC	2 cut(s)	1137, 1645
MroI	TCCGGA	1 cut(s)	1402
MseI	TTAA	8 cut(s)	120, 281, 696, 804, 876, 1319, 1733, 2018
MspA1I	CMGCKG	1 cut(s)	238
Mva1269I	GAATGC	1 cut(s)	1516
MvaI	CCWGG	5 cut(s)	290, 395, 1276, 1719, 1860
MvnI	CGCG	2 cut(s)	250, 329
MwoI	GCNNNNNNNGC	6 cut(s)	235, 247, 442, 899, 989, 1646
NciI	CCSGG	7 cut(s)	112, 161, 167, 194, 367, 1178, 2207
NcoI	CCATGG	3 cut(s)	1497, 2115, 2279
NdeII	GATC	7 cut(s)	65, 580, 1399, 1435, 1667, 1957, 2172
NlaIV	GGNNCC	6 cut(s)	165, 486, 687, 738, 1007, 1401
NmeAIII	GCCGAG	1 cut(s)	2243
NmuCI	GTSAC	6 cut(s)	755, 1161, 1486, 1868, 1916, 1921
PagI	TCATGA	1 cut(s)	2077
PceI	AGGCCT	2 cut(s)	245, 1751
PcsI	WCGNNNNNNNCGW	2 cut(s)	387, 2031
PctI	GAATGC	1 cut(s)	1516
PfeI	GAWTC	3 cut(s)	337, 791, 1808
Pfl23II	CGTACG	1 cut(s)	2098
PfoI	TCCNGGA	1 cut(s)	1858
PinAI	ACCGGT	1 cut(s)	1109
PkrI	GCNGC	2 cut(s)	240, 991
PleI	GAGTC	2 cut(s)	194, 1168
PpsI	GAGTC	2 cut(s)	194, 1168
PshBI	ATTAAT	1 cut(s)	1733
Psp6I	CCWGG	5 cut(s)	288, 393, 1274, 1717, 1858
PspEI	GGTNACC	1 cut(s)	2286
PspGI	CCWGG	5 cut(s)	288, 393, 1274, 1717, 1858
PspLI	CGTACG	1 cut(s)	2098
PspN4I	GGNNCC	6 cut(s)	165, 486, 687, 738, 1007, 1401
PspPI	GGNCC	6 cut(s)	140, 163, 205, 484, 566, 1107
PsrI	GAACNNNNNNTAC	2 cut(s)	2024, 2056
PsuI	RGATCY	3 cut(s)	65, 1399, 1957
RsaI	GTAC	5 cut(s)	155, 1007, 1123, 2100, 2234
RsaNI	GTAC	5 cut(s)	154, 1006, 1122, 2099, 2233
SaqAI	TTAA	8 cut(s)	120, 281, 696, 804, 876, 1319, 1733, 2018
SatI	GCNGC	2 cut(s)	239, 990
Sau3AI	GATC	7 cut(s)	65, 580, 1399, 1435, 1667, 1957, 2172
Sau96I	GGNCC	6 cut(s)	140, 163, 205, 484, 566, 1107
ScaI	AGTACT	1 cut(s)	155
SchI	GAGTC	2 cut(s)	194, 1169
SduI	GDGCHC	1 cut(s)	741
SfaNI	GCATC	5 cut(s)	1171, 1203, 1357, 1699, 1777
SinI	GGWCC	3 cut(s)	163, 484, 1107
SseBI	AGGCCT	2 cut(s)	245, 1751
SsiI	CCGC	6 cut(s)	182, 238, 256, 470, 887, 971
SspMI	CTAG	4 cut(s)	669, 675, 903, 1797
StuI	AGGCCT	2 cut(s)	245, 1751
StyI	CCWWGG	4 cut(s)	585, 1497, 2115, 2279
TaaI	ACNGT	3 cut(s)	1126, 1846, 2232
TaiI	ACGT	6 cut(s)	321, 1521, 1923, 1937, 2104, 2238
TaqI	TCGA	5 cut(s)	390, 579, 794, 1013, 2175
TaqII	GACCGA	1 cut(s)	860
TatI	WGTACW	1 cut(s)	153
TauI	GCSGC	1 cut(s)	241
TfiI	GAWTC	3 cut(s)	337, 791, 1808
Tru1I	TTAA	8 cut(s)	120, 281, 696, 804, 876, 1319, 1733, 2018
Tru9I	TTAA	8 cut(s)	120, 281, 696, 804, 876, 1319, 1733, 2018
TscAI	CASTG	2 cut(s)	598, 1032
TseFI	GTSAC	6 cut(s)	755, 1161, 1486, 1868, 1916, 1921
TseI	GCWGC	1 cut(s)	989
Tsp45I	GTSAC	6 cut(s)	755, 1161, 1486, 1868, 1916, 1921
TspDTI	ATGAA	6 cut(s)	106, 1104, 1299, 1968, 2066, 2175
TspGWI	ACGGA	2 cut(s)	225, 337
TspRI	CASTG	2 cut(s)	598, 1032
VpaK11BI	GGWCC	3 cut(s)	163, 484, 1107
VspI	ATTAAT	1 cut(s)	1733
XagI	CCTNNNNNAGG	1 cut(s)	1795
XapI	RAATTY	7 cut(s)	575, 628, 780, 1198, 1315, 1441, 2273
XcmI	CCANNNNNNNNNTGG	1 cut(s)	316
XspI	CTAG	4 cut(s)	669, 675, 903, 1797
ZraI	GACGTC	1 cut(s)	1935
ZrmI	AGTACT	1 cut(s)	155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.