Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0006369

Physical Location & Seq

Genomic Coordinates Forward (+)

52790582 .. 52795198

Gene Structure 4617 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00258420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGGCCTTCACTTTCCTCTCAATCCCTCACTTTCTCTCTCCCCAACCCAACTTTCTCTCTCTAAAACCCCAATTCGCCTCTCTCCGACCCTCCATCTTCTCCCTCCCGGCCCGTCCACTCAAACCCGCCCACGCAATCGGCCCCGACGGCAAGTACTACCCGGACCCCTCCGACGACGACCCGCCCGAAGCCCCTGAAGACTCCGGCCACGGCGTCTCCAAATTCCAGCAAATCCAGCGCCAGGCCTCCCGCCACCAGAAGCTCCAGGAGGACGACTTCAAGAAGCACCAGAACACCATGCTCTCCGCCATTGCTGACGTCGAGGACCCGCCGGAGAACCCCGGCTCCGGAAATACCCCCAGCTCCGGGGACGATTTGTTCGGGGACATCGACGAAGCTATTGCCTTGAAGCGCAAGGAGTTTGTGAAGCAAGGGCTTTTGAAGCCGAACCCTAAGGTGGAGGCGGTGGAGGAGCTGGAGCCGGAGGAGGTTGTGGACTTGGAGGAGATTGATGAACTTCAGGGGCTGGCGATTGTGAGTGAGGATTCGGATGAGGAAGGGACTGAGAAATTCGACTCAAAGGTTAGTGATTTGGATGAGAAAGATGGAGTTTCGGTTTCGAGTTCGTTGTTTGATATGGATTTTGATAATTATGGTAAGACTAGGGCTAGAATTGTGGAACCTAAGTTTAAAATGAGCTTAGCTGAGCTTTTGGATGAGAGTAAAGTAGTGCCGATTTCGGTTTATGGTGATTTAGAGGTGGAGATAACCGGAATTCAGCATGATTCGAGGGTGGTTAGTGCCGGGGATTTGTTTGTGTGCTGTGTTGGGAGGAAAACCGATGGTCATTTGTATTTGAGTGAGGCTATTAAGAGAGGAGCGGTGGCTGTAGTAGCTAGTAAGGAGATATACATGGAGGAAAGCTTGGGGTGCAAGGCATTGGTCATTGTGGAAGATACCAATGCTGATTTACCAGCATTGGCTGCATCGTTTTATAGGTATCCGTCGAAGAATATGTCGGTGATTGGGATAACGGGGACGAATGGGAAGACGACATCAGCGTATTTGATAAAGGGGATGTATGAAGCGATGGGATTGAGGACGGGTATGTTGAGCACAGTTGCGTATTACGTACATGGAGATAATAAGTTGGAGTCGCCTAATACAACCCCGGATGCTGTTTTGGTTCAAAATATGATGGCCAAGATGCTCCATAATGGGGCTGAAGCAGTAGTCATGGAGGCTTCTTCTCACGGGCTAGCTCTGTCTAGGTGTGATGAAGTTGATATTGACATAGCGGTTTTTACAAATTTGACGAGAGACCATTTGGATTTTCACGTGACTGAGGAGGAGTATAGGGAGGCAAAGGGTCAGTTGTTTTCAAGGATGGTTGATCCAGAACGGCACAGGAAAGTTGTCAACATTGATGACCCAAATGCACCTTTCTTCATTTCACAGGGGAACCCAGATGTTCCAGTTGTGACCTTTGCCATGGAGAATAAGAATGCAGATGTCTATCCCTTGAAGTTTGAACTTTCTTTGTTTGAGACACAGGTTTTAGTCAATACTCCCAATGGTATATTGGAGATATCATCAGGGTTGCTTGGACGGCATAACATTTACAATATACTGGCTTCAGTAGCAGTTGGGATTGCAGTTGGAGCACCATTAGAAGACATCGTTAGGGGAATTGAAGAGGTTGATGCTGTTCCAGGGAGGTGTGAGTTGATAGACGAGGAACAGGCATTTGGTGTGATTGTGGATTATGCTCACACTCCTGATGCCTTGTCCAGGCTATTGGATTCTGTACGGGAGCTTGGTGCAAGGAGGATTATTACTGTAATCGGTTGTCCTGGGGAGAGTGACAGAGGGAAAAGACCTATGATGGCGAAGATTGCAACAGATAAAAGTGATGTGACAATACTGACGTCTGACAATCCAAAGAATGAAGATCCATTGGACATCTTGGATGATATGCTGGCTGGTATAGGATGGACAATGCAGGAATACTTGAAACATGGCGAGAATGATTACTACCCACCTCTTCCAAATGGTCATAGGATTTTCCTGCATGATATAAGACGAGTAGCTGTACGGTGTGCTGTTGCCATGGGTGAGGAAGGTGATATGGTTGTTGTTGCGGGCAAAGGCCATGAAGCGTATCAGATAGAAGGGGACAAAAAGAGTTCTTTGATGACCGGGAAGAGTGTCGAGAGGCATTACAGTATGTTGATGAGCTTCACCAAGCTGGAATTGACACAAGTGAATTCCCATGGAGGTTACCTGAGAGTCATTGACCTCTTCCAAAAGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009295 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0010468 GO:0016043 GO:0019222 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044424 GO:0044444 GO:0044464 GO:0048285 GO:0050789 GO:0060255 GO:0065007 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mur_ligase Mur_ligase_C Mur_ligase_M

Protein Analysis

771

Amino Acids

84.91

Weight (kDa)

4.78

Isoelectric Point (pI)

37.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mur_ligase	PF01225	255 - 317	2.5e-08	Mur ligase family, catalytic domain
Mur_ligase_M	PF08245	344 - 549	7.1e-55	Mur ligase middle domain
Mur_ligase_C	PF02875	572 - 717	2.2e-25	Mur ligase, glutamate ligase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006369)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63680 AT1G63680 AT1G63680
fragaria_vesca	FvH4_5g02610 FvH4_5g02610 FvH4_5g02610 FvH4_5g02610
malus_domestica	MD06G1085100.v1.1 MD14G1106100.v1.1 MD14G1106300.v1.1
prunus_persica	Prupe.5G101100_v2.0.a1
pyrus_communis	pycom06g08120
rosa_chinensis	RchiOBHm_Chr7g0197491
rosa_laevigata	RLG00000003936 RLG00000003970
rosa_multiflora	Rmu_co8124254.1_g000001 Rmu_co8184758.1_g000001 Rmu_sc0002167.1_g000003 Rmu_sc0013361.1_g000005
rosa_roxburghii	Rroxscaffold_3G00258420
rosa_rugosa	Rorug07G0040000 Rorug07G0040100
rosa_samantha	Rh7AG165200 Rh7BG167300 Rh7CG173700 Rh7DG166600
rosa_wichuraiana	Rw7G014400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	321, 1931
AccBSI	CCGCTC	1 cut(s)	881
AccIII	TCCGGA	1 cut(s)	347
AciI	CCGC	9 cut(s)	126, 182, 250, 306, 329, 464, 881, 1298, 2141
AclWI	GGATC	2 cut(s)	1388, 1946
AcoI	YGGCCR	2 cut(s)	205, 1200
AcsI	RAATTY	5 cut(s)	221, 569, 774, 1309, 2266
AcuI	CTGAAG	4 cut(s)	216, 503, 1245, 1620
AcvI	CACGTG	1 cut(s)	1339
AcyI	GRCGYC	3 cut(s)	213, 318, 1928
AfaI	GTAC	4 cut(s)	155, 1134, 1809, 2094
AfiI	CCNNNNNNNGG	7 cut(s)	241, 347, 365, 366, 457, 1219, 1791
AgsI	TTSAA	9 cut(s)	280, 409, 442, 1190, 1383, 1525, 1532, 1694, 2014
AjnI	CCWGG	5 cut(s)	240, 264, 1711, 1790, 1852
AjuI	GAANNNNNNNTTGG	4 cut(s)	36, 68, 1731, 1763
AloI	GAACNNNNNNTCC	4 cut(s)	329, 361, 362, 394
Alw21I	GWGCWC	2 cut(s)	1118, 1666
Alw26I	GTCTC	3 cut(s)	220, 1314, 1541
AlwI	GGATC	2 cut(s)	1388, 1946
AlwNI	CAGNNNCTG	1 cut(s)	526
Aor13HI	TCCGGA	1 cut(s)	347
AoxI	GGCC	7 cut(s)	3, 108, 139, 205, 243, 1200, 2149
ApeKI	GCWGC	1 cut(s)	983
ApoI	RAATTY	5 cut(s)	221, 569, 774, 1309, 2266
ArsI	GACNNNNNNTTYG	4 cut(s)	362, 394, 1001, 1033
AspLEI	GCGC	2 cut(s)	240, 414
AspS9I	GGNCC	4 cut(s)	109, 140, 163, 325
AsuC2I	CCSGG	7 cut(s)	107, 161, 342, 367, 805, 1172, 2200
AsuHPI	GGTGA	5 cut(s)	761, 1033, 2126, 2135, 2233
AsuNHI	GCTAGC	1 cut(s)	1258
AvaII	GGWCC	2 cut(s)	163, 325
AxyI	CCTNAGG	1 cut(s)	453
BalI	TGGCCA	1 cut(s)	1202
BbrPI	CACGTG	1 cut(s)	1339
BbsI	GAAGAC	3 cut(s)	204, 1055, 1680
Bbv12I	GWGCWC	2 cut(s)	1118, 1666
BbvI	GCAGC	1 cut(s)	970
BccI	CCATC	8 cut(s)	101, 599, 836, 1084, 1192, 1381, 1879, 1986
BceAI	ACGGC	4 cut(s)	163, 226, 1418, 1625
BcgI	CGANNNNNNTGC	2 cut(s)	383, 417
BciT130I	CCWGG	5 cut(s)	242, 266, 1713, 1792, 1854
BciVI	GTATCC	1 cut(s)	1011
BcnI	CCSGG	7 cut(s)	107, 161, 342, 367, 805, 1172, 2200
BcoDI	GTCTC	3 cut(s)	220, 1314, 1541
BfaI	CTAG	5 cut(s)	663, 669, 897, 1259, 1269
BfmI	CTRYAG	1 cut(s)	888
BfoI	RGCGCY	1 cut(s)	241
BfuI	GTATCC	1 cut(s)	1011
BglI	GCCNNNNNGGC	1 cut(s)	147
BisI	GCNGC	1 cut(s)	984
BlpI	GCTNAGC	2 cut(s)	700, 705
BlsI	GCNGC	1 cut(s)	985
BmcAI	AGTACT	1 cut(s)	155
Bme18I	GGWCC	2 cut(s)	163, 325
BmgT120I	GGNCC	4 cut(s)	109, 140, 163, 325
BmiI	GGNNCC	7 cut(s)	142, 165, 327, 346, 480, 681, 1463
BmsI	GCATC	5 cut(s)	995, 1165, 1197, 1693, 1771
BmtI	GCTAGC	1 cut(s)	1262
BpiI	GAAGAC	3 cut(s)	204, 1055, 1680
BpmI	CTGGAG	2 cut(s)	248, 497
Bpu1102I	GCTNAGC	2 cut(s)	700, 705
BpuMI	CCSGG	7 cut(s)	107, 161, 342, 367, 805, 1172, 2200
BsaAI	YACGTR	2 cut(s)	1132, 1339
BsaBI	GATNNNNATC	1 cut(s)	1515
BsaHI	GRCGYC	3 cut(s)	213, 318, 1928
BsaI	GGTCTC	1 cut(s)	1314
BsaWI	WCCGGW	2 cut(s)	347, 770
BsaXI	ACNNNNNCTCC	6 cut(s)	200, 230, 329, 359, 600, 630
Bsc4I	CCNNNNNNNGG	7 cut(s)	241, 347, 365, 366, 457, 1219, 1791
Bse1I	ACTGG	2 cut(s)	1475, 1635
Bse21I	CCTNAGG	1 cut(s)	453
Bse3DI	GCAATG	1 cut(s)	309
Bse8I	GATNNNNATC	1 cut(s)	1515
BseAI	TCCGGA	1 cut(s)	347
BseBI	CCWGG	5 cut(s)	242, 266, 1713, 1792, 1854
BseGI	GGATG	8 cut(s)	556, 601, 721, 1083, 1180, 1392, 1975, 1997
BseJI	GATNNNNATC	1 cut(s)	1515
BseLI	CCNNNNNNNGG	7 cut(s)	241, 347, 365, 366, 457, 1219, 1791
BseMI	GCAATG	1 cut(s)	309
BseMII	CTCAG	4 cut(s)	555, 696, 1335, 2276
BseNI	ACTGG	2 cut(s)	1475, 1635
BseRI	GAGGAG	6 cut(s)	485, 500, 518, 891, 1361, 1364
BseXI	GCAGC	1 cut(s)	970
BseYI	CCCAGC	1 cut(s)	359
BshFI	GGCC	7 cut(s)	5, 110, 141, 207, 245, 1202, 2151
BsiHKAI	GWGCWC	2 cut(s)	1118, 1666
BslFI	GGGAC	5 cut(s)	383, 398, 574, 1051, 2189
BslI	CCNNNNNNNGG	7 cut(s)	241, 347, 365, 366, 457, 1219, 1791
BsmAI	GTCTC	3 cut(s)	220, 1314, 1541
BsmBI	CGTCTC	1 cut(s)	220
BsmFI	GGGAC	5 cut(s)	383, 398, 574, 1051, 2189
BsmI	GAATGC	1 cut(s)	1510
BsnI	GGCC	7 cut(s)	5, 110, 141, 207, 245, 1202, 2151
Bso31I	GGTCTC	1 cut(s)	1314
Bsp1286I	GDGCHC	2 cut(s)	1118, 1666
Bsp13I	TCCGGA	1 cut(s)	347
Bsp143I	GATC	2 cut(s)	1393, 1951
Bsp1720I	GCTNAGC	2 cut(s)	700, 705
Bsp19I	CCATGG	3 cut(s)	1491, 2109, 2272
BspACI	CCGC	9 cut(s)	126, 182, 250, 306, 329, 464, 881, 1298, 2141
BspANI	GGCC	7 cut(s)	5, 110, 141, 207, 245, 1202, 2151
BspCNI	CTCAG	4 cut(s)	556, 697, 1336, 2277
BspEI	TCCGGA	1 cut(s)	347
BspLI	GGNNCC	7 cut(s)	142, 165, 327, 346, 480, 681, 1463
BspOI	GCTAGC	1 cut(s)	1262
BspPI	GGATC	2 cut(s)	1388, 1946
BspTNI	GGTCTC	1 cut(s)	1314
BsrBI	CCGCTC	1 cut(s)	881
BsrDI	GCAATG	1 cut(s)	309
BsrI	ACTGG	2 cut(s)	1475, 1635
BssMI	GATC	2 cut(s)	1393, 1951
BssNI	GRCGYC	3 cut(s)	213, 318, 1928
BssT1I	CCWWGG	3 cut(s)	1491, 2109, 2272
Bst2UI	CCWGG	5 cut(s)	242, 266, 1713, 1792, 1854
Bst4CI	ACNGT	4 cut(s)	1120, 1840, 2097, 2225
Bst6I	CTCTTC	4 cut(s)	1689, 2049, 2198, 2306
BstACI	GRCGYC	3 cut(s)	213, 318, 1928
BstAPI	GCANNNNNTGC	1 cut(s)	983
BstBAI	YACGTR	2 cut(s)	1132, 1339
BstC8I	GCNNGC	4 cut(s)	528, 1260, 1980, 2143
BstDEI	CTNAG	7 cut(s)	453, 564, 684, 700, 705, 1344, 2285
BstDSI	CCRYGG	4 cut(s)	208, 1491, 2109, 2272
BstEII	GGTNACC	1 cut(s)	2279
BstENI	CCTNNNNNAGG	1 cut(s)	1789
BstF5I	GGATG	8 cut(s)	556, 601, 721, 1083, 1180, 1392, 1975, 1997
BstH2I	RGCGCY	1 cut(s)	241
BstHHI	GCGC	2 cut(s)	240, 414
BstKTI	GATC	2 cut(s)	1396, 1954
BstMAI	GTCTC	3 cut(s)	220, 1314, 1541
BstMBI	GATC	2 cut(s)	1393, 1951
BstNI	CCWGG	5 cut(s)	242, 266, 1713, 1792, 1854
BstPI	GGTNACC	1 cut(s)	2279
BstSFI	CTRYAG	1 cut(s)	888
BstSNI	TACGTA	1 cut(s)	1132
BstV1I	GCAGC	1 cut(s)	970
BstV2I	GAAGAC	3 cut(s)	204, 1055, 1680
BstX2I	RGATCY	1 cut(s)	1951
BstXI	CCANNNNNNTGG	1 cut(s)	1798
BstYI	RGATCY	1 cut(s)	1951
Bsu36I	CCTNAGG	1 cut(s)	453
BsuI	GTATCC	1 cut(s)	1011
BsuRI	GGCC	7 cut(s)	5, 110, 141, 207, 245, 1202, 2151
BtgI	CCRYGG	4 cut(s)	208, 1491, 2109, 2272
BtgZI	GCGATG	1 cut(s)	1103
BtsCI	GGATG	8 cut(s)	556, 601, 721, 1083, 1180, 1392, 1975, 1997
Cac8I	GCNNGC	4 cut(s)	528, 1260, 1980, 2143
CaiI	CAGNNNCTG	1 cut(s)	526
CfoI	GCGC	2 cut(s)	240, 414
Cfr13I	GGNCC	4 cut(s)	109, 140, 163, 325
CseI	GACGC	1 cut(s)	202
Csp6I	GTAC	4 cut(s)	154, 1133, 1808, 2093
CviQI	GTAC	4 cut(s)	154, 1133, 1808, 2093
DdeI	CTNAG	7 cut(s)	453, 564, 684, 700, 705, 1344, 2285
DpnI	GATC	2 cut(s)	1395, 1953
DpnII	GATC	2 cut(s)	1393, 1951
DraI	TTTAAA	1 cut(s)	691
EaeI	YGGCCR	2 cut(s)	205, 1200
Eam1104I	CTCTTC	4 cut(s)	1689, 2049, 2198, 2306
EarI	CTCTTC	4 cut(s)	1689, 2049, 2198, 2306
EciI	GGCGGA	1 cut(s)	295
Eco105I	TACGTA	1 cut(s)	1132
Eco130I	CCWWGG	3 cut(s)	1491, 2109, 2272
Eco147I	AGGCCT	1 cut(s)	245
Eco31I	GGTCTC	1 cut(s)	1314
Eco32I	GATATC	1 cut(s)	1590
Eco47I	GGWCC	2 cut(s)	163, 325
Eco57I	CTGAAG	4 cut(s)	216, 503, 1245, 1620
Eco72I	CACGTG	1 cut(s)	1339
Eco81I	CCTNAGG	1 cut(s)	453
Eco91I	GGTNACC	1 cut(s)	2279
EcoNI	CCTNNNNNAGG	1 cut(s)	1789
EcoO109I	RGGNCCY	1 cut(s)	325
EcoO65I	GGTNACC	1 cut(s)	2279
EcoRI	GAATTC	2 cut(s)	774, 2266
EcoRII	CCWGG	5 cut(s)	240, 264, 1711, 1790, 1852
EcoRV	GATATC	1 cut(s)	1590
EcoT14I	CCWWGG	3 cut(s)	1491, 2109, 2272
ErhI	CCWWGG	3 cut(s)	1491, 2109, 2272
Esp3I	CGTCTC	1 cut(s)	220
FaqI	GGGAC	5 cut(s)	383, 398, 574, 1051, 2189
FauI	CCCGC	5 cut(s)	133, 189, 257, 336, 2134
Fnu4HI	GCNGC	1 cut(s)	984
FokI	GGATG	8 cut(s)	563, 608, 728, 1090, 1187, 1399, 1982, 2004
Fsp4HI	GCNGC	1 cut(s)	984
FspBI	CTAG	5 cut(s)	663, 669, 897, 1259, 1269
GlaI	GCGC	2 cut(s)	239, 413
GluI	GCNGC	1 cut(s)	984
GsaI	CCCAGC	1 cut(s)	363
GsuI	CTGGAG	2 cut(s)	248, 497
HaeII	RGCGCY	1 cut(s)	241
HaeIII	GGCC	7 cut(s)	5, 110, 141, 207, 245, 1202, 2151
HgaI	GACGC	1 cut(s)	202
HhaI	GCGC	2 cut(s)	240, 414
Hin1I	GRCGYC	3 cut(s)	213, 318, 1928
Hin6I	GCGC	2 cut(s)	238, 412
HinP1I	GCGC	2 cut(s)	238, 412
HincII	GTYRAC	1 cut(s)	1420
HindII	GTYRAC	1 cut(s)	1420
HindIII	AAGCTT	1 cut(s)	922
HinfI	GANTC	7 cut(s)	200, 545, 575, 785, 1154, 1802, 2289
HphI	GGTGA	5 cut(s)	761, 1033, 2126, 2135, 2233
Hpy166II	GTNNAC	3 cut(s)	116, 496, 1420
Hpy188I	TCNGA	5 cut(s)	86, 172, 550, 1933, 2166
Hpy188III	TCNNGA	5 cut(s)	280, 348, 1397, 1778, 2212
Hpy8I	GTNNAC	3 cut(s)	116, 496, 1420
Hpy99I	CGWCG	6 cut(s)	149, 176, 179, 323, 395, 1009
HpyAV	CCTTC	4 cut(s)	16, 551, 2114, 2165
HpyCH4III	ACNGT	4 cut(s)	1120, 1840, 2097, 2225
HpyCH4IV	ACGT	4 cut(s)	318, 1131, 1338, 1928
HpyCH4V	TGCA	9 cut(s)	933, 986, 1439, 1508, 1655, 1823, 1898, 2002, 2071
HpyF3I	CTNAG	7 cut(s)	453, 564, 684, 700, 705, 1344, 2285
HpySE526I	ACGT	4 cut(s)	318, 1131, 1338, 1928
Hsp92I	GRCGYC	3 cut(s)	213, 318, 1928
HspAI	GCGC	2 cut(s)	238, 412
Kpn2I	TCCGGA	1 cut(s)	347
Kzo9I	GATC	2 cut(s)	1393, 1951
LmnI	GCTCC	9 cut(s)	267, 350, 368, 472, 478, 878, 1215, 1661, 1813
Lsp1109I	GCAGC	1 cut(s)	970
LweI	GCATC	5 cut(s)	995, 1165, 1197, 1693, 1771
MaeI	CTAG	5 cut(s)	663, 669, 897, 1259, 1269
MaeII	ACGT	4 cut(s)	318, 1131, 1338, 1928
MaeIII	GTNAC	5 cut(s)	1339, 1480, 1862, 1915, 2279
MalI	GATC	2 cut(s)	1395, 1953
MbiI	CCGCTC	1 cut(s)	881
MboI	GATC	2 cut(s)	1393, 1951
MflI	RGATCY	1 cut(s)	1951
MhlI	GDGCHC	2 cut(s)	1118, 1666
MlsI	TGGCCA	1 cut(s)	1202
MluNI	TGGCCA	1 cut(s)	1202
MlyI	GAGTC	4 cut(s)	194, 569, 1163, 2298
MmeI	TCCRAC	4 cut(s)	109, 195, 1131, 1639
Mox20I	TGGCCA	1 cut(s)	1202
MroI	TCCGGA	1 cut(s)	347
MscI	TGGCCA	1 cut(s)	1202
MseI	TTAA	2 cut(s)	690, 870
Msp20I	TGGCCA	1 cut(s)	1202
Mva1269I	GAATGC	1 cut(s)	1510
MvaI	CCWGG	5 cut(s)	242, 266, 1713, 1792, 1854
NciI	CCSGG	7 cut(s)	107, 161, 342, 367, 805, 1172, 2200
NcoI	CCATGG	3 cut(s)	1491, 2109, 2272
NdeII	GATC	2 cut(s)	1393, 1951
NheI	GCTAGC	1 cut(s)	1258
NlaIV	GGNNCC	7 cut(s)	142, 165, 327, 346, 480, 681, 1463
NmuCI	GTSAC	4 cut(s)	1339, 1480, 1862, 1915
PceI	AGGCCT	1 cut(s)	245
PcsI	WCGNNNNNNNCGW	3 cut(s)	183, 387, 1058
PctI	GAATGC	1 cut(s)	1510
PfeI	GAWTC	3 cut(s)	545, 785, 1802
PfoI	TCCNGGA	1 cut(s)	264
PkrI	GCNGC	1 cut(s)	985
PleI	GAGTC	4 cut(s)	194, 569, 1162, 2297
PmaCI	CACGTG	1 cut(s)	1339
PmlI	CACGTG	1 cut(s)	1339
PpsI	GAGTC	4 cut(s)	194, 569, 1162, 2297
Ppu21I	YACGTR	2 cut(s)	1132, 1339
PpuMI	RGGWCCY	1 cut(s)	325
Psp5II	RGGWCCY	1 cut(s)	325
Psp6I	CCWGG	5 cut(s)	240, 264, 1711, 1790, 1852
PspCI	CACGTG	1 cut(s)	1339
PspEI	GGTNACC	1 cut(s)	2279
PspFI	CCCAGC	1 cut(s)	359
PspGI	CCWGG	5 cut(s)	240, 264, 1711, 1790, 1852
PspN4I	GGNNCC	7 cut(s)	142, 165, 327, 346, 480, 681, 1463
PspPI	GGNCC	4 cut(s)	109, 140, 163, 325
PspPPI	RGGWCCY	1 cut(s)	325
PstNI	CAGNNNCTG	1 cut(s)	526
PsuI	RGATCY	1 cut(s)	1951
RsaI	GTAC	4 cut(s)	155, 1134, 1809, 2094
RsaNI	GTAC	4 cut(s)	154, 1133, 1808, 2093
SaqAI	TTAA	2 cut(s)	690, 870
SatI	GCNGC	1 cut(s)	984
Sau3AI	GATC	2 cut(s)	1393, 1951
Sau96I	GGNCC	4 cut(s)	109, 140, 163, 325
ScaI	AGTACT	1 cut(s)	155
SchI	GAGTC	4 cut(s)	194, 569, 1163, 2298
SduI	GDGCHC	2 cut(s)	1118, 1666
SfaNI	GCATC	5 cut(s)	995, 1165, 1197, 1693, 1771
SfcI	CTRYAG	1 cut(s)	888
SinI	GGWCC	2 cut(s)	163, 325
SnaBI	TACGTA	1 cut(s)	1132
SseBI	AGGCCT	1 cut(s)	245
SsiI	CCGC	9 cut(s)	126, 182, 250, 306, 329, 464, 881, 1298, 2141
SspMI	CTAG	5 cut(s)	663, 669, 897, 1259, 1269
StuI	AGGCCT	1 cut(s)	245
StyI	CCWWGG	3 cut(s)	1491, 2109, 2272
TaaI	ACNGT	4 cut(s)	1120, 1840, 2097, 2225
TaiI	ACGT	4 cut(s)	321, 1134, 1341, 1931
TaqI	TCGA	7 cut(s)	321, 390, 573, 620, 788, 1007, 2211
TatI	WGTACW	1 cut(s)	153
TfiI	GAWTC	3 cut(s)	545, 785, 1802
Tru1I	TTAA	2 cut(s)	690, 870
Tru9I	TTAA	2 cut(s)	690, 870
TseFI	GTSAC	4 cut(s)	1339, 1480, 1862, 1915
TseI	GCWGC	1 cut(s)	983
Tsp45I	GTSAC	4 cut(s)	1339, 1480, 1862, 1915
TspDTI	ATGAA	6 cut(s)	528, 1098, 1293, 1438, 1962, 2169
TspGWI	ACGGA	1 cut(s)	993
VpaK11BI	GGWCC	2 cut(s)	163, 325
XagI	CCTNNNNNAGG	1 cut(s)	1789
XapI	RAATTY	5 cut(s)	221, 569, 774, 1309, 2266
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1579
XspI	CTAG	5 cut(s)	663, 669, 897, 1259, 1269
ZraI	GACGTC	2 cut(s)	319, 1929
ZrmI	AGTACT	1 cut(s)	155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.