Rw4G010900

Eukaryotic protein of unknown function (DUF842)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013059
Physical Location & Seq
Reverse (-)
25529006 .. 25532083
3078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010900.1

Sequence Viewer

Length: 450 bp
ATGGATCACCTGGCAGCGATGGAAGAGCAATTGGTATCCGAGAGGGTTAAACAAAGGCTACAAGACGTGAATGCGGCTGCCCAAAAGTATCTCTCTCCGGTCCAAGACCATGTCAATTTCACCCTCCAGCAAGCATACTTTAAATGTGCATACGAGTGTTTTGATAGGAGGAGAAGCGAACAAGAGATTGCCAATTGCGTTGAAAATTGTAGCGTTCCTGTTGTTAGTGCTCAACAGAGAGTTGAGAATGAGATGGCCAAGTTTCAAGAAAGGTTGAGTAGGGCACTGATGGTCTGTCAAGACAAGTTTGATGCATCTAAGCTTCAACAGGTCAAAACTAGTGCACTCAATGATTTGGAGTCATGTGTTCAACAAACAACCGAAGATGGAATAAGGACACTTCCACTTCTTGCTGAAAGATTGAAGTCTTCCTTTTCGATCAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF842

Protein Analysis

149

Amino Acids

17.06

Weight (kDa)

5.45

Isoelectric Point (pI)

63.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF842 PF05811 20 - 143 7.7e-37 Eukaryotic protein of unknown function (DUF842)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05730 AT1G05730 AT2G31725
fragaria_vesca FvH4_3g34590 FvH4_3g34590
malus_domestica MD04G1037100.v1.1
prunus_persica Prupe.1G185700_v2.0.a1
pyrus_communis pycom04g03020
rosa_chinensis RchiOBHm_Chr4g0405841
rosa_laevigata RLG00000008821
rosa_multiflora Rmu_sc0001200.1_g000015
rosa_roxburghii Rroxscaffold_5G00350400
rosa_rugosa Rorug04G0062500
rosa_samantha Rh4AG133900 Rh4BG129000 Rh4CG141700 Rh4DG128400
rosa_wichuraiana Rw4G010900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 74
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 255
AgsI TTSAA 5 cut(s) 203, 266, 326, 371, 424
AhlI ACTAGT 1 cut(s) 338
AjiI CACGTC 1 cut(s) 67
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 1 cut(s) 322
AluI AGCT 1 cut(s) 322
Alw21I GWGCWC 2 cut(s) 232, 346
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 255
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 14, 77
AspS9I GGNCC 1 cut(s) 100
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 1 cut(s) 100
BaeGI GKGCMC 2 cut(s) 286, 346
BalI TGGCCA 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 232, 346
BbvI GCAGC 2 cut(s) 26, 64
BccI CCATC 4 cut(s) 13, 247, 283, 380
BciT130I CCWGG 1 cut(s) 11
BciVI GTATCC 1 cut(s) 46
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 1 cut(s) 339
BfuI GTATCC 1 cut(s) 46
BisI GCNGC 3 cut(s) 15, 75, 78
BlsI GCNGC 3 cut(s) 16, 76, 79
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 100
BmgBI CACGTC 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 100
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 2 cut(s) 301, 323
BpiI GAAGAC 1 cut(s) 420
BpmI CTGGAG 1 cut(s) 110
BsaWI WCCGGW 1 cut(s) 97
BseBI CCWGG 1 cut(s) 11
BseRI GAGGAG 1 cut(s) 184
BseSI GKGCMC 2 cut(s) 286, 346
BseXI GCAGC 2 cut(s) 26, 64
BshFI GGCC 1 cut(s) 257
BsiHKAI GWGCWC 2 cut(s) 232, 346
BsiSI CCGG 1 cut(s) 98
BsmI GAATGC 1 cut(s) 76
BsnI GGCC 1 cut(s) 257
Bsp1286I GDGCHC 3 cut(s) 232, 286, 346
Bsp143I GATC 2 cut(s) 4, 438
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 257
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 18
BssMI GATC 2 cut(s) 4, 438
Bst2UI CCWGG 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 1 cut(s) 318
BstKTI GATC 2 cut(s) 7, 441
BstMBI GATC 2 cut(s) 4, 438
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 9
BstSLI GKGCMC 2 cut(s) 286, 346
BstV1I GCAGC 2 cut(s) 26, 64
BstV2I GAAGAC 1 cut(s) 420
BsuI GTATCC 1 cut(s) 46
BsuRI GGCC 1 cut(s) 257
BtgZI GCGATG 1 cut(s) 32
BtrI CACGTC 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 100
CviAII CATG 2 cut(s) 110, 363
CviJI RGCY 4 cut(s) 58, 77, 257, 322
CviKI_1 RGCY 4 cut(s) 58, 77, 257, 322
DdeI CTNAG 1 cut(s) 318
DpnI GATC 2 cut(s) 6, 440
DpnII GATC 2 cut(s) 4, 438
DraI TTTAAA 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 316
FaeI CATG 2 cut(s) 113, 366
FaiI YATR 4 cut(s) 111, 136, 151, 364
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FatI CATG 2 cut(s) 109, 362
Fnu4HI GCNGC 3 cut(s) 15, 75, 78
Fsp4HI GCNGC 3 cut(s) 15, 75, 78
FspBI CTAG 1 cut(s) 339
GluI GCNGC 3 cut(s) 15, 75, 78
GsuI CTGGAG 1 cut(s) 110
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 98
Hin1II CATG 2 cut(s) 113, 366
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 1 cut(s) 359
HpaII CCGG 1 cut(s) 98
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 344
Hpy188I TCNGA 1 cut(s) 40
Hpy188III TCNNGA 2 cut(s) 266, 299
Hpy8I GTNNAC 1 cut(s) 344
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 3 cut(s) 149, 314, 344
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 2 cut(s) 113, 366
Kzo9I GATC 2 cut(s) 4, 438
LguI GCTCTTC 1 cut(s) 18
LpnPI CCDG 5 cut(s) 23, 111, 140, 231, 314
Lsp1109I GCAGC 2 cut(s) 26, 64
LweI GCATC 2 cut(s) 301, 323
MaeI CTAG 1 cut(s) 339
MaeII ACGT 1 cut(s) 66
MalI GATC 2 cut(s) 6, 440
MboI GATC 2 cut(s) 4, 438
MboII GAAGA 3 cut(s) 35, 395, 420
MfeI CAATTG 2 cut(s) 29, 193
MhlI GDGCHC 3 cut(s) 232, 286, 346
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 5 cut(s) 29, 115, 193, 205, 445
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 368
MnlI CCTC 3 cut(s) 36, 134, 162
Mox20I TGGCCA 1 cut(s) 257
Mph1103I ATGCAT 1 cut(s) 316
MscI TGGCCA 1 cut(s) 257
MseI TTAA 3 cut(s) 48, 141, 448
MslI CAYNNNNRTG 1 cut(s) 154
Msp20I TGGCCA 1 cut(s) 257
MspI CCGG 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 11
MunI CAATTG 2 cut(s) 29, 193
Mva1269I GAATGC 1 cut(s) 76
MvaI CCWGG 1 cut(s) 11
NdeII GATC 2 cut(s) 4, 438
NlaIII CATG 2 cut(s) 113, 366
NsiI ATGCAT 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 18
PctI GAATGC 1 cut(s) 76
PflFI GACNNNGTC 1 cut(s) 110
PkrI GCNGC 3 cut(s) 16, 76, 79
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspPI GGNCC 1 cut(s) 100
PsyI GACNNNGTC 1 cut(s) 110
RseI CAYNNNNRTG 1 cut(s) 154
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 3 cut(s) 48, 141, 448
SatI GCNGC 3 cut(s) 15, 75, 78
Sau3AI GATC 2 cut(s) 4, 438
Sau96I GGNCC 1 cut(s) 100
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 3 cut(s) 232, 286, 346
SetI ASST 5 cut(s) 12, 69, 275, 324, 333
SfaNI GCATC 2 cut(s) 301, 323
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 154
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 5 cut(s) 29, 115, 193, 205, 445
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 9
TaiI ACGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 437
TasI AATT 5 cut(s) 29, 115, 193, 205, 445
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 3 cut(s) 48, 141, 448
Tru9I TTAA 3 cut(s) 48, 141, 448
TscAI CASTG 1 cut(s) 291
TseI GCWGC 2 cut(s) 14, 77
TspRI CASTG 1 cut(s) 291
Tth111I GACNNNGTC 1 cut(s) 110
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 100
XspI CTAG 1 cut(s) 339
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.