Rw4G013840

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016305
Physical Location & Seq
Reverse (-)
32371324 .. 32373209
1886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013840.1

Sequence Viewer

Length: 1743 bp
ATGATACGCTCAAGTCCTCGTGCTGTTCTTGAAACCACATATAGGCTTGTTCTATCAAAATCCACACACAACTTGGCCACATTAGAACAGACTTGCTCTGATCTGTTACGTACATGTGCCCAATCCTCCAACTTACCCAATGGCAGAGCAATTCATGGAAAACTCTTCAAAGGGTCTCTGCCCTTTTCGCCGTTCCTTCAAAATCATCTACTCAACATGTATGTCAAATGTGGCCATCTCAACAATGCCCTCCAACTGTTCGACGAAATGCCTGAGAGAAATGTTGTGTCTTGGTCTGCGCTCATTAAAGGGTTTGTGCAACACGGCTGCCCCAATGAAGCCCTGTCTTTGTTTGGTCGTATGCACAGGGATGGCACCACCATGCCCAATGAGTTCACATTGGTCAGCTCCCTCCAGGCATGCTCCTTGTGTGGGAATCTGACTCAAGCTTATCAGGTTTACGCATTCATTGTTCGTCTCGGGTTCGAGTGGAATGTGTTCCTCACTAATGCATTGTTGACTGTTTTAGTAAGACATGGTGAACTAACAGAGGCTCTGCAAGTGTTTGAGAACTGCCCCAATAAAGATATAGTGTCTTGGAATGCTATCATGGCTGGCTACTTGGAGTATGATTGTTCAGAAGTAGCTACATTCTGGTGGAGAATGAATCGCGAGGGAGTGACGCCCGATAGCTACACATTTTCAAGTGTGCTCACTGGGTTGGCTGCTGTCACTGATCTTAAAATGGGTGTGCAGGTCCATGCACAGCTTGTGAGGTATGGCCATGGGGCGGAGATTTGTGTGGGGAATTCCCTGGCTGATATGTATATCAAAAATCATCAGTTGTTGGATGGTTTCAAAGCTTTTCATGAGATGCCTTTAAAAGATGTGTGCTCATGGACTGAAATGGCTGCTGGCTGCCTGCAATGTGGGGAACCAAGCAAAGCACTCGAGGTTATTGCTCAAATGAAGAAAGTTGGGATACAGCCAAACAAGTTCACACTTGCAACTGCCCTGAATGCGTGTGCCAATCTGGCTTCGTTGGAGGAAGGGAAGAAATTCCATGGTTTGAGAATTAAACTTGAAACTTGTACTGATGTGGACATCTGTGTAGATAATGCTCTGCTGGACATGTATGCAAAATGTGGATGCATGGATGGTGCACGGAGTGTTTTTGAGTCGATGAAGGATAGTTCTGTAGTCTCATGGACCACAATGATAATGGGGTGCGCACAAAATGGGAAGGCTAGAGAAGCTCTTGAGGTTTTTGATAAGATGAGGCTGCAGGAAGGCATTGAGCCAAATTACATCACCTTTATTTGTGTGCTTTATGCATGTAGCCAAGGAGGGTTTATTGATGAGGGATGGAAGTACTTTGCCTCCATGACTCAAAACTTTGGAATCACCCCAGGAGAAGACCATTATGCATGCATGGTTGATCTTCTAGGCCGTGCTGGACGTATAAAGGAAGCTGAGGAATTGATCAAGAACATGCCTTTAAAACCAGGTGTTTTGGTTTGGCAGACTTTGCTTGGTGCTTGTCAGCTGCTTGGGGATACAGAAACAGGAAGGCGCGCAGCAGAGCATGCATTAGAGATAAACAGAACAGAGCCATCAACATATATATTGCTGTCAAACATTTTTGCTGGTTTGAGCAATTGGGACAGCGTCGGGATGCTGAGGAAACTAATGGACTCTAGGGATGTAAAGAAATTGCCTGGATCCAGTTGGATTGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

580

Amino Acids

64.45

Weight (kDa)

6.04

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 93 - 141 5.3e-07 PPR repeat family
PPR PF01535 96 - 125 7.8e-08 PPR repeat
PPR_2 PF13041 195 - 242 1.3e-07 PPR repeat family
PPR_2 PF13041 295 - 344 2.4e-08 PPR repeat family
PPR_2 PF13041 399 - 447 9.4e-10 PPR repeat family
PPR_3 PF13812 399 - 446 2.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 400 - 427 6.2e-06 PPR repeat
PPR PF01535 401 - 427 1.1e-08 PPR repeat
PPR_long PF17177 406 - 544 3.9e-08 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 519 - 579 4.2e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016305)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12540 FvH4_4g12540
malus_domestica MD14G1245400.v1.1
prunus_persica Prupe.5G243000_v2.0.a1
pyrus_communis pycom14g20640
rosa_chinensis RchiOBHm_Chr4g0411381
rosa_laevigata RLG00000008361
rosa_multiflora Rmu_sc0000629.1_g000021
rosa_roxburghii Rroxscaffold_5G00355960
rosa_rugosa Rorug04G0109600
rosa_samantha Rh4BG164500 Rh4CG179100 Rh4DG162200
rosa_wichuraiana Rw4G013840 Rw4G013940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1231
Acc36I ACCTGC 1 cut(s) 745
AccB1I GGYRCC 1 cut(s) 374
AccII CGCG 2 cut(s) 672, 1575
AciI CCGC 1 cut(s) 791
AclWI GGATC 2 cut(s) 1716, 1729
AcoI YGGCCR 3 cut(s) 75, 232, 781
AcsI RAATTY 2 cut(s) 808, 1058
AcyI GRCGYC 1 cut(s) 683
AdeI CACNNNGTG 1 cut(s) 1169
AfaI GTAC 3 cut(s) 112, 1093, 1373
AfiI CCNNNNNNNGG 3 cut(s) 42, 432, 790
AflIII ACRYGT 3 cut(s) 113, 216, 1131
AgsI TTSAA 7 cut(s) 32, 169, 200, 705, 859, 1085, 1736
AjnI CCWGG 5 cut(s) 414, 813, 1408, 1504, 1717
AluBI AGCT 9 cut(s) 408, 449, 647, 693, 769, 863, 1256, 1472, 1546
AluI AGCT 9 cut(s) 408, 449, 647, 693, 769, 863, 1256, 1472, 1546
Alw21I GWGCWC 3 cut(s) 714, 896, 1165
Alw26I GTCTC 3 cut(s) 180, 482, 1207
Alw44I GTGCAC 1 cut(s) 1161
AlwI GGATC 2 cut(s) 1716, 1729
Ama87I CYCGRG 2 cut(s) 479, 950
AoxI GGCC 4 cut(s) 75, 232, 781, 1447
ApaLI GTGCAC 1 cut(s) 1161
ApeKI GCWGC 7 cut(s) 327, 725, 911, 918, 1282, 1546, 1577
ApoI RAATTY 2 cut(s) 808, 1058
Asp700I GAANNNNTTC 2 cut(s) 497, 1058
AspLEI GCGC 4 cut(s) 301, 1232, 1575, 1577
AspS9I GGNCC 2 cut(s) 757, 1209
AsuHPI GGTGA 3 cut(s) 551, 1303, 1396
AvaI CYCGRG 2 cut(s) 479, 950
AvaII GGWCC 2 cut(s) 757, 1209
BaeGI GKGCMC 2 cut(s) 121, 1165
BalI TGGCCA 3 cut(s) 77, 234, 783
BamHI GGATCC 1 cut(s) 1721
BanI GGYRCC 1 cut(s) 374
BauI CACGAG 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 1422
Bbv12I GWGCWC 3 cut(s) 714, 896, 1165
BbvCI CCTCAGC 2 cut(s) 1473, 1679
BbvI GCAGC 7 cut(s) 314, 712, 898, 905, 1269, 1533, 1589
BccI CCATC 6 cut(s) 243, 365, 845, 1151, 1359, 1621
BceAI ACGGC 3 cut(s) 175, 340, 1434
BcgI CGANNNNNNTGC 4 cut(s) 931, 941, 965, 975
BciT130I CCWGG 5 cut(s) 416, 815, 1410, 1506, 1719
BciVI GTATCC 2 cut(s) 975, 1549
BclI TGATCA 1 cut(s) 1482
BcoDI GTCTC 3 cut(s) 180, 482, 1207
BfaI CTAG 3 cut(s) 1248, 1445, 1698
BfmI CTRYAG 2 cut(s) 1197, 1283
BfuAI ACCTGC 1 cut(s) 745
BfuI GTATCC 2 cut(s) 975, 1549
BglI GCCNNNNNGGC 1 cut(s) 1034
BisI GCNGC 7 cut(s) 328, 726, 912, 919, 1283, 1547, 1578
BlsI GCNGC 7 cut(s) 329, 727, 913, 920, 1284, 1548, 1579
BmcAI AGTACT 1 cut(s) 1373
Bme1390I CCNGG 5 cut(s) 416, 815, 1410, 1506, 1719
Bme18I GGWCC 2 cut(s) 757, 1209
BmeT110I CYCGRG 2 cut(s) 479, 950
BmgT120I GGNCC 2 cut(s) 757, 1209
BmiI GGNNCC 3 cut(s) 376, 936, 1723
BmrFI CCNGG 5 cut(s) 416, 815, 1410, 1506, 1719
BmrI ACTGGG 1 cut(s) 726
BmsI GCATC 3 cut(s) 864, 1139, 1665
BmuI ACTGGG 1 cut(s) 726
BpiI GAAGAC 1 cut(s) 1422
BpmI CTGGAG 1 cut(s) 398
Bpu10I CCTNAGC 2 cut(s) 1473, 1679
BpuEI CTTGAG 2 cut(s) 429, 1280
BsaAI YACGTR 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 683
BsaI GGTCTC 1 cut(s) 180
BsaJI CCNNGG 5 cut(s) 784, 813, 1063, 1342, 1408
BsaXI ACNNNNNCTCC 2 cut(s) 1364, 1394
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 432, 790
Bse1I ACTGG 2 cut(s) 721, 1725
Bse3DI GCAATG 1 cut(s) 932
BseBI CCWGG 5 cut(s) 416, 815, 1410, 1506, 1719
BseDI CCNNGG 5 cut(s) 784, 813, 1063, 1342, 1408
BseGI GGATG 7 cut(s) 376, 856, 1154, 1162, 1370, 1680, 1708
BseLI CCNNNNNNNGG 3 cut(s) 42, 432, 790
BseMI GCAATG 1 cut(s) 932
BseMII CTCAG 3 cut(s) 264, 1464, 1670
BseNI ACTGG 2 cut(s) 721, 1725
BsePI GCGCGC 1 cut(s) 1573
BseSI GKGCMC 2 cut(s) 121, 1165
BseXI GCAGC 7 cut(s) 314, 712, 898, 905, 1269, 1533, 1589
BsgI GTGCAG 1 cut(s) 773
Bsh1236I CGCG 2 cut(s) 672, 1575
BshFI GGCC 4 cut(s) 77, 234, 783, 1449
BshNI GGYRCC 1 cut(s) 374
BsiHKAI GWGCWC 3 cut(s) 714, 896, 1165
BsiHKCI CYCGRG 2 cut(s) 479, 950
BslFI GGGAC 1 cut(s) 1676
BslI CCNNNNNNNGG 3 cut(s) 42, 432, 790
BsmAI GTCTC 3 cut(s) 180, 482, 1207
BsmBI CGTCTC 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 1676
BsmI GAATGC 3 cut(s) 464, 607, 1024
BsnI GGCC 4 cut(s) 77, 234, 783, 1449
Bso31I GGTCTC 1 cut(s) 180
BsoBI CYCGRG 2 cut(s) 479, 950
Bsp1286I GDGCHC 4 cut(s) 121, 714, 896, 1165
Bsp143I GATC 5 cut(s) 100, 736, 1438, 1482, 1721
Bsp19I CCATGG 2 cut(s) 784, 1063
Bsp68I TCGCGA 1 cut(s) 672
BspACI CCGC 1 cut(s) 791
BspANI GGCC 4 cut(s) 77, 234, 783, 1449
BspCNI CTCAG 3 cut(s) 265, 1465, 1671
BspFNI CGCG 2 cut(s) 672, 1575
BspHI TCATGA 1 cut(s) 868
BspLI GGNNCC 3 cut(s) 376, 936, 1723
BspMAI CTGCAG 1 cut(s) 1287
BspMI ACCTGC 1 cut(s) 745
BspPI GGATC 2 cut(s) 1716, 1729
BspT107I GGYRCC 1 cut(s) 374
BspTNI GGTCTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 932
BsrI ACTGG 2 cut(s) 721, 1725
BssECI CCNNGG 5 cut(s) 784, 813, 1063, 1342, 1408
BssHII GCGCGC 1 cut(s) 1573
BssMI GATC 5 cut(s) 100, 736, 1438, 1482, 1721
BssNI GRCGYC 1 cut(s) 683
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 3 cut(s) 784, 1063, 1342
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 5 cut(s) 416, 815, 1410, 1506, 1719
Bst4CI ACNGT 2 cut(s) 258, 523
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 1 cut(s) 683
BstAPI GCANNNNNTGC 2 cut(s) 1528, 1586
BstBAI YACGTR 1 cut(s) 110
BstC8I GCNNGC 7 cut(s) 421, 616, 916, 923, 1429, 1575, 1587
BstDEI CTNAG 3 cut(s) 273, 1473, 1679
BstDSI CCRYGG 2 cut(s) 784, 1063
BstF5I GGATG 7 cut(s) 376, 856, 1154, 1162, 1370, 1680, 1708
BstFNI CGCG 2 cut(s) 672, 1575
BstHHI GCGC 4 cut(s) 301, 1232, 1575, 1577
BstKTI GATC 5 cut(s) 103, 739, 1441, 1485, 1724
BstMAI GTCTC 3 cut(s) 180, 482, 1207
BstMBI GATC 5 cut(s) 100, 736, 1438, 1482, 1721
BstMWI GCNNNNNNNGC 7 cut(s) 187, 611, 1019, 1034, 1253, 1528, 1586
BstNI CCWGG 5 cut(s) 416, 815, 1410, 1506, 1719
BstNSI RCATGY 8 cut(s) 117, 220, 423, 1135, 1338, 1431, 1495, 1589
BstSCI CCNGG 5 cut(s) 414, 813, 1408, 1504, 1717
BstSFI CTRYAG 2 cut(s) 1197, 1283
BstSLI GKGCMC 2 cut(s) 121, 1165
BstSNI TACGTA 1 cut(s) 110
BstUI CGCG 2 cut(s) 672, 1575
BstV1I GCAGC 7 cut(s) 314, 712, 898, 905, 1269, 1533, 1589
BstV2I GAAGAC 1 cut(s) 1422
BstX2I RGATCY 1 cut(s) 1721
BstYI RGATCY 1 cut(s) 1721
BsuI GTATCC 2 cut(s) 975, 1549
BsuRI GGCC 4 cut(s) 77, 234, 783, 1449
BtgI CCRYGG 2 cut(s) 784, 1063
BtsCI GGATG 7 cut(s) 376, 856, 1154, 1162, 1370, 1680, 1708
BtsIMutI CAGTG 2 cut(s) 714, 732
BtuMI TCGCGA 1 cut(s) 672
BveI ACCTGC 1 cut(s) 745
Cac8I GCNNGC 7 cut(s) 421, 616, 916, 923, 1429, 1575, 1587
CciI TCATGA 1 cut(s) 868
CfoI GCGC 4 cut(s) 301, 1232, 1575, 1577
Cfr13I GGNCC 2 cut(s) 757, 1209
CseI GACGC 2 cut(s) 691, 1657
CsiI ACCWGGT 1 cut(s) 1504
Csp6I GTAC 3 cut(s) 111, 1092, 1372
CviQI GTAC 3 cut(s) 111, 1092, 1372
DdeI CTNAG 3 cut(s) 273, 1473, 1679
DpnI GATC 5 cut(s) 102, 738, 1440, 1484, 1723
DpnII GATC 5 cut(s) 100, 736, 1438, 1482, 1721
DraI TTTAAA 2 cut(s) 882, 1500
DraIII CACNNNGTG 1 cut(s) 1169
EaeI YGGCCR 3 cut(s) 75, 232, 781
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
EciI GGCGGA 1 cut(s) 806
Eco105I TACGTA 1 cut(s) 110
Eco130I CCWWGG 3 cut(s) 784, 1063, 1342
Eco31I GGTCTC 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 757, 1209
Eco88I CYCGRG 2 cut(s) 479, 950
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 5 cut(s) 414, 813, 1408, 1504, 1717
EcoT14I CCWWGG 3 cut(s) 784, 1063, 1342
EcoT22I ATGCAT 6 cut(s) 514, 1154, 1336, 1429, 1433, 1591
ErhI CCWWGG 3 cut(s) 784, 1063, 1342
Esp3I CGTCTC 1 cut(s) 482
FaqI GGGAC 1 cut(s) 1676
FbaI TGATCA 1 cut(s) 1482
Fnu4HI GCNGC 7 cut(s) 328, 726, 912, 919, 1283, 1547, 1578
FokI GGATG 7 cut(s) 383, 863, 1161, 1169, 1377, 1687, 1715
Fsp4HI GCNGC 7 cut(s) 328, 726, 912, 919, 1283, 1547, 1578
FspAI RTGCGCAY 1 cut(s) 1231
FspBI CTAG 3 cut(s) 1248, 1445, 1698
FspI TGCGCA 1 cut(s) 1231
GlaI GCGC 4 cut(s) 300, 1231, 1574, 1576
GluI GCNGC 7 cut(s) 328, 726, 912, 919, 1283, 1547, 1578
GsuI CTGGAG 1 cut(s) 398
HaeIII GGCC 4 cut(s) 77, 234, 783, 1449
HgaI GACGC 2 cut(s) 691, 1657
HhaI GCGC 4 cut(s) 301, 1232, 1575, 1577
Hin1I GRCGYC 1 cut(s) 683
Hin6I GCGC 4 cut(s) 299, 1230, 1573, 1575
HinP1I GCGC 4 cut(s) 299, 1230, 1573, 1575
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HindIII AAGCTT 2 cut(s) 447, 861
HinfI GANTC 7 cut(s) 436, 442, 667, 1178, 1387, 1401, 1694
HphI GGTGA 3 cut(s) 551, 1303, 1396
Hpy166II GTNNAC 7 cut(s) 396, 460, 519, 542, 999, 1102, 1163
Hpy188I TCNGA 3 cut(s) 100, 441, 640
Hpy188III TCNNGA 6 cut(s) 29, 671, 869, 1259, 1486, 1672
Hpy8I GTNNAC 7 cut(s) 396, 460, 519, 542, 999, 1102, 1163
Hpy99I CGWCG 2 cut(s) 266, 1673
HpyAV CCTTC 6 cut(s) 206, 1043, 1180, 1237, 1283, 1563
HpyCH4III ACNGT 2 cut(s) 258, 523
HpyCH4IV ACGT 2 cut(s) 109, 1459
HpyF10VI GCNNNNNNNGC 7 cut(s) 187, 611, 1019, 1034, 1253, 1528, 1586
HpyF3I CTNAG 3 cut(s) 273, 1473, 1679
HpySE526I ACGT 2 cut(s) 109, 1459
Hsp92I GRCGYC 1 cut(s) 683
HspAI GCGC 4 cut(s) 299, 1230, 1573, 1575
Ksp22I TGATCA 1 cut(s) 1482
Kzo9I GATC 5 cut(s) 100, 736, 1438, 1482, 1721
LmnI GCTCC 2 cut(s) 413, 428
Lsp1109I GCAGC 7 cut(s) 314, 712, 898, 905, 1269, 1533, 1589
LweI GCATC 3 cut(s) 864, 1139, 1665
MabI ACCWGGT 1 cut(s) 1504
MaeI CTAG 3 cut(s) 1248, 1445, 1698
MaeII ACGT 2 cut(s) 109, 1459
MaeIII GTNAC 3 cut(s) 105, 679, 730
MalI GATC 5 cut(s) 102, 738, 1440, 1484, 1723
MboI GATC 5 cut(s) 100, 736, 1438, 1482, 1721
MboII GAAGA 5 cut(s) 157, 982, 1066, 1427, 1433
MfeI CAATTG 1 cut(s) 1657
MflI RGATCY 1 cut(s) 1721
MhlI GDGCHC 4 cut(s) 121, 714, 896, 1165
MlsI TGGCCA 3 cut(s) 77, 234, 783
MluCI AATT 8 cut(s) 150, 808, 1058, 1074, 1303, 1478, 1657, 1712
MluNI TGGCCA 3 cut(s) 77, 234, 783
MlyI GAGTC 4 cut(s) 436, 1187, 1381, 1688
MmeI TCCRAC 5 cut(s) 153, 277, 828, 1023, 1709
Mox20I TGGCCA 3 cut(s) 77, 234, 783
Mph1103I ATGCAT 6 cut(s) 514, 1154, 1336, 1429, 1433, 1591
MroXI GAANNNNTTC 2 cut(s) 497, 1058
MscI TGGCCA 3 cut(s) 77, 234, 783
MseI TTAA 5 cut(s) 306, 741, 881, 1077, 1499
MslI CAYNNNNRTG 3 cut(s) 369, 380, 655
Msp20I TGGCCA 3 cut(s) 77, 234, 783
MspA1I CMGCKG 1 cut(s) 1546
MspR9I CCNGG 5 cut(s) 416, 815, 1410, 1506, 1719
MunI CAATTG 1 cut(s) 1657
Mva1269I GAATGC 3 cut(s) 464, 607, 1024
MvaI CCWGG 5 cut(s) 416, 815, 1410, 1506, 1719
MvnI CGCG 2 cut(s) 672, 1575
MwoI GCNNNNNNNGC 7 cut(s) 187, 611, 1019, 1034, 1253, 1528, 1586
NcoI CCATGG 2 cut(s) 784, 1063
NdeII GATC 5 cut(s) 100, 736, 1438, 1482, 1721
NlaIV GGNNCC 3 cut(s) 376, 936, 1723
NmuCI GTSAC 2 cut(s) 679, 730
NruI TCGCGA 1 cut(s) 672
NsbI TGCGCA 1 cut(s) 1231
NsiI ATGCAT 6 cut(s) 514, 1154, 1336, 1429, 1433, 1591
NspI RCATGY 8 cut(s) 117, 220, 423, 1135, 1338, 1431, 1495, 1589
PaeI GCATGC 3 cut(s) 423, 1431, 1589
PaeR7I CTCGAG 1 cut(s) 950
PagI TCATGA 1 cut(s) 868
PauI GCGCGC 1 cut(s) 1573
PciI ACATGT 3 cut(s) 113, 216, 1131
PctI GAATGC 3 cut(s) 464, 607, 1024
PdmI GAANNNNTTC 2 cut(s) 497, 1058
PfeI GAWTC 3 cut(s) 436, 667, 1401
PflFI GACNNNGTC 1 cut(s) 1667
PkrI GCNGC 7 cut(s) 329, 727, 913, 920, 1284, 1548, 1579
PleI GAGTC 4 cut(s) 436, 1186, 1381, 1688
PpsI GAGTC 4 cut(s) 436, 1186, 1381, 1688
Ppu21I YACGTR 1 cut(s) 110
PscI ACATGT 3 cut(s) 113, 216, 1131
Psp6I CCWGG 5 cut(s) 414, 813, 1408, 1504, 1717
PspGI CCWGG 5 cut(s) 414, 813, 1408, 1504, 1717
PspN4I GGNNCC 3 cut(s) 376, 936, 1723
PspPI GGNCC 2 cut(s) 757, 1209
PspXI VCTCGAGB 1 cut(s) 950
PstI CTGCAG 1 cut(s) 1287
PsuI RGATCY 1 cut(s) 1721
PsyI GACNNNGTC 1 cut(s) 1667
PteI GCGCGC 1 cut(s) 1573
PvuII CAGCTG 1 cut(s) 1546
RruI TCGCGA 1 cut(s) 672
RsaI GTAC 3 cut(s) 112, 1093, 1373
RsaNI GTAC 3 cut(s) 111, 1092, 1372
RseI CAYNNNNRTG 3 cut(s) 369, 380, 655
SaqAI TTAA 5 cut(s) 306, 741, 881, 1077, 1499
SatI GCNGC 7 cut(s) 328, 726, 912, 919, 1283, 1547, 1578
Sau3AI GATC 5 cut(s) 100, 736, 1438, 1482, 1721
Sau96I GGNCC 2 cut(s) 757, 1209
ScaI AGTACT 1 cut(s) 1373
SchI GAGTC 4 cut(s) 436, 1187, 1381, 1688
ScrFI CCNGG 5 cut(s) 416, 815, 1410, 1506, 1719
SduI GDGCHC 4 cut(s) 121, 714, 896, 1165
SexAI ACCWGGT 1 cut(s) 1504
SfaNI GCATC 3 cut(s) 864, 1139, 1665
SfcI CTRYAG 2 cut(s) 1197, 1283
Sfr274I CTCGAG 1 cut(s) 950
SinI GGWCC 2 cut(s) 757, 1209
SlaI CTCGAG 1 cut(s) 950
SmiMI CAYNNNNRTG 3 cut(s) 369, 380, 655
SmlI CTYRAG 4 cut(s) 10, 444, 950, 1259
SmoI CTYRAG 4 cut(s) 10, 444, 950, 1259
SnaBI TACGTA 1 cut(s) 110
SphI GCATGC 3 cut(s) 423, 1431, 1589
Sse9I AATT 8 cut(s) 150, 808, 1058, 1074, 1303, 1478, 1657, 1712
SsiI CCGC 1 cut(s) 791
SspMI CTAG 3 cut(s) 1248, 1445, 1698
StyD4I CCNGG 5 cut(s) 414, 813, 1408, 1504, 1717
StyI CCWWGG 3 cut(s) 784, 1063, 1342
TaaI ACNGT 2 cut(s) 258, 523
TaiI ACGT 2 cut(s) 112, 1462
TaqI TCGA 4 cut(s) 261, 486, 951, 1181
TasI AATT 8 cut(s) 150, 808, 1058, 1074, 1303, 1478, 1657, 1712
TatI WGTACW 2 cut(s) 1091, 1371
TfiI GAWTC 3 cut(s) 436, 667, 1401
Tru1I TTAA 5 cut(s) 306, 741, 881, 1077, 1499
Tru9I TTAA 5 cut(s) 306, 741, 881, 1077, 1499
TscAI CASTG 2 cut(s) 721, 739
TseFI GTSAC 2 cut(s) 679, 730
TseI GCWGC 7 cut(s) 327, 725, 911, 918, 1282, 1546, 1577
Tsp45I GTSAC 2 cut(s) 679, 730
TspDTI ATGAA 7 cut(s) 143, 351, 457, 680, 857, 983, 1199
TspGWI ACGGA 1 cut(s) 1180
TspRI CASTG 2 cut(s) 721, 739
Tth111I GACNNNGTC 1 cut(s) 1667
VneI GTGCAC 1 cut(s) 1161
VpaK11BI GGWCC 2 cut(s) 757, 1209
XapI RAATTY 2 cut(s) 808, 1058
XceI RCATGY 8 cut(s) 117, 220, 423, 1135, 1338, 1431, 1495, 1589
XcmI CCANNNNNNNNNTGG 2 cut(s) 70, 1219
XhoI CTCGAG 1 cut(s) 950
XmnI GAANNNNTTC 2 cut(s) 497, 1058
XspI CTAG 3 cut(s) 1248, 1445, 1698
ZrmI AGTACT 1 cut(s) 1373
Zsp2I ATGCAT 6 cut(s) 514, 1154, 1336, 1429, 1433, 1591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.