Rw4G013940

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016305
Physical Location & Seq
Reverse (-)
32602976 .. 32604714
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013940.1

Sequence Viewer

Length: 1674 bp
ATGATACGCTCAAGTCCTCGTGCTGTTCTTGAAACCACATATAGGCTTGTTCTATCAAAATCCACACACAACTTGGCCACATTAGAACAGACTTGCTCTGATCTGTTACGTACATGTGCCCAATCCTCCAACTTACCCAATGGCAGAGCAATTCATGGAAAACTCTTCAAAGGGTCTCTGCCCTTTTCGCCGTTCCTTCAAAATCATCTACTCAACATGTATGTCAAATGTGGCCATCTCAACAATGCCCTCCAACTGTTCGACGAAATGCCTGAGAGAAATGTTGTGTCTTGGTCTGCGCTCATTAAAGGGTTTGTGCAACACGGCTGCCCCAATGAAGCCCTGTCTTTGTTTGGTCGTATGCACAGGGATGGCACCACCATGCCCAATGAGTTCACATTGGTCAGCTCCCTCCAGGCATGCTCCTTGTGTGGGAATCTGACTCAAGCTTATCAGGTTTACGCATTCATTGTTCGTCTCGGGTTCGAGTGGAATGTGTTCCTCACTAATGCATTGTTGACTGTTTTAGTAAGACATGGTGAACTAACAGAGGCTCTGCAAGTGTTTGAGAACTGCCCCAATAAAGATATAGTGTCTTGGAATGCTATCATGGCTGGCTACTTGGAGTATGATTGTTCAGAAGTAGCTACATTCTGCTACACATTTTCAAGTGTGCTCACTGGGTTGGCTGCTGTCACTGATCTTAAAATGGGTGTGCAGGTCCATGCACAGCTTGTGAGGTATGGCCATGGGGCGGAGATTTGTGTGGGGAATTCCCTGGCTGATATGTATATCAAAAATCATCAGTTGTTGGATGGTTTCAAAGCTTTTCATGAGATGCCTTTAAAAGATGTGTGCTCATGGACTGAAATGGCTGCTGGCTGCCTGCAATGTGGGGAACCAAGCAAAGCACTCGAGGTTATTGCTCAAATGAAGAAAGTTGGGATACAGCCAAACAAGTTCACACTTGCAACTGCCCTGAATGCGTGTGCCAATCTGGCTTCGTTGGAGGAAGGGAAGAAATTCCATGGTTTGAGAATTAAACTTGAAACTTGTACTGATGTGGACATCTGTGTAGATAATGCTCTGCTGGACATGTATGCAAAATGTGGATGCATGGATGGTGCACGGAGTGTTTTTGAGTCGATGAAGGATAGTTCTGTAGTCTCATGGACCACAATGATAATGGGGTGCGCACAAAATGGGAAGGCTGGAGAAGCTCTTGAGGTTTTTGATAAGATGAGGCTGCAGGAAGGCATTGAGCCAAATTACATCACCTTTATTTGTGTGCTTTATGCATGTAGCCAAGGAGGGTTTATTGATGAGGGATGGAAGTACTTTGACTCCATGACTCAAAGCTTTGGAATCACCCCAGGAGAAGACCATTATGCATGCATGGTGGATCTTCTAGGCCGTGCTGGACGTATAAAGGAAGCTGAGGAATTGATCAAGAACATGCCTTTAAAACCAGGTGTTTTGGTTTGGCAGACTTTGCTTGGTGCTTGCGCAGCAGAGCATGCATTAGAGATAAACAGAACAGAGCCATCAACATATATATTGCTGTCAAACATTTTTGCTGGTTTGAGCAATTGGGACAGCGTCGGGATGCTGAGGAAACTAATGGACTCTAGGGATGTAAAGAAATTGCCTGGATCCAGTTGGATTGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

557

Amino Acids

61.62

Weight (kDa)

5.87

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 93 - 141 5.1e-07 PPR repeat family
PPR PF01535 96 - 125 7.4e-08 PPR repeat
PPR_2 PF13041 283 - 332 2.3e-08 PPR repeat family
PPR_2 PF13041 387 - 435 2.9e-09 PPR repeat family
PPR_3 PF13812 387 - 434 1e-06 Pentatricopeptide repeat domain
PPR PF01535 389 - 415 3.4e-08 PPR repeat
PPR_long PF17177 394 - 487 3.9e-07 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 499 - 556 2.8e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016305)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12540 FvH4_4g12540
malus_domestica MD14G1245400.v1.1
prunus_persica Prupe.5G243000_v2.0.a1
pyrus_communis pycom14g20640
rosa_chinensis RchiOBHm_Chr4g0411381
rosa_laevigata RLG00000008361
rosa_multiflora Rmu_sc0000629.1_g000021
rosa_roxburghii Rroxscaffold_5G00355960
rosa_rugosa Rorug04G0109600
rosa_samantha Rh4BG164500 Rh4CG179100 Rh4DG162200
rosa_wichuraiana Rw4G013840 Rw4G013940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1195, 1507
Acc36I ACCTGC 1 cut(s) 709
AccB1I GGYRCC 1 cut(s) 374
AciI CCGC 1 cut(s) 755
AclWI GGATC 3 cut(s) 1410, 1647, 1660
AcoI YGGCCR 3 cut(s) 75, 232, 745
AcsI RAATTY 2 cut(s) 772, 1022
AdeI CACNNNGTG 1 cut(s) 1133
AfaI GTAC 3 cut(s) 112, 1057, 1337
AfiI CCNNNNNNNGG 3 cut(s) 42, 432, 754
AflIII ACRYGT 3 cut(s) 113, 216, 1095
AgsI TTSAA 7 cut(s) 32, 169, 200, 669, 823, 1049, 1667
AjnI CCWGG 5 cut(s) 414, 777, 1372, 1468, 1648
AluBI AGCT 8 cut(s) 408, 449, 647, 733, 827, 1220, 1359, 1436
AluI AGCT 8 cut(s) 408, 449, 647, 733, 827, 1220, 1359, 1436
Alw21I GWGCWC 3 cut(s) 678, 860, 1129
Alw26I GTCTC 3 cut(s) 180, 482, 1171
Alw44I GTGCAC 1 cut(s) 1125
AlwI GGATC 3 cut(s) 1410, 1647, 1660
Ama87I CYCGRG 2 cut(s) 479, 914
AoxI GGCC 4 cut(s) 75, 232, 745, 1411
ApaLI GTGCAC 1 cut(s) 1125
ApeKI GCWGC 6 cut(s) 327, 689, 875, 882, 1246, 1508
ApoI RAATTY 2 cut(s) 772, 1022
Asp700I GAANNNNTTC 2 cut(s) 497, 1022
AspLEI GCGC 3 cut(s) 301, 1196, 1508
AspS9I GGNCC 2 cut(s) 721, 1173
AsuHPI GGTGA 3 cut(s) 551, 1267, 1360
AvaI CYCGRG 2 cut(s) 479, 914
AvaII GGWCC 2 cut(s) 721, 1173
BaeGI GKGCMC 2 cut(s) 121, 1129
BalI TGGCCA 3 cut(s) 77, 234, 747
BamHI GGATCC 1 cut(s) 1652
BanI GGYRCC 1 cut(s) 374
BauI CACGAG 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 1386
Bbv12I GWGCWC 3 cut(s) 678, 860, 1129
BbvCI CCTCAGC 2 cut(s) 1437, 1610
BbvI GCAGC 6 cut(s) 314, 676, 862, 869, 1233, 1520
BccI CCATC 6 cut(s) 243, 365, 809, 1115, 1323, 1552
BceAI ACGGC 3 cut(s) 175, 340, 1398
BcgI CGANNNNNNTGC 4 cut(s) 895, 905, 929, 939
BciT130I CCWGG 5 cut(s) 416, 779, 1374, 1470, 1650
BciVI GTATCC 1 cut(s) 939
BclI TGATCA 1 cut(s) 1446
BcoDI GTCTC 3 cut(s) 180, 482, 1171
BfaI CTAG 2 cut(s) 1409, 1629
BfmI CTRYAG 2 cut(s) 1161, 1247
BfuAI ACCTGC 1 cut(s) 709
BfuI GTATCC 1 cut(s) 939
BglI GCCNNNNNGGC 1 cut(s) 998
BisI GCNGC 6 cut(s) 328, 690, 876, 883, 1247, 1509
BlsI GCNGC 6 cut(s) 329, 691, 877, 884, 1248, 1510
BmcAI AGTACT 1 cut(s) 1337
Bme1390I CCNGG 5 cut(s) 416, 779, 1374, 1470, 1650
Bme18I GGWCC 2 cut(s) 721, 1173
BmeT110I CYCGRG 2 cut(s) 479, 914
BmgT120I GGNCC 2 cut(s) 721, 1173
BmiI GGNNCC 3 cut(s) 376, 900, 1654
BmrFI CCNGG 5 cut(s) 416, 779, 1374, 1470, 1650
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 3 cut(s) 828, 1103, 1596
BmuI ACTGGG 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 1386
BpmI CTGGAG 2 cut(s) 398, 1233
Bpu10I CCTNAGC 2 cut(s) 1437, 1610
BpuEI CTTGAG 2 cut(s) 429, 1244
BsaAI YACGTR 1 cut(s) 110
BsaI GGTCTC 1 cut(s) 180
BsaJI CCNNGG 5 cut(s) 748, 777, 1027, 1306, 1372
BsaXI ACNNNNNCTCC 2 cut(s) 1328, 1358
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 432, 754
Bse1I ACTGG 2 cut(s) 685, 1656
Bse3DI GCAATG 1 cut(s) 896
BseBI CCWGG 5 cut(s) 416, 779, 1374, 1470, 1650
BseDI CCNNGG 5 cut(s) 748, 777, 1027, 1306, 1372
BseGI GGATG 7 cut(s) 376, 820, 1118, 1126, 1334, 1611, 1639
BseLI CCNNNNNNNGG 3 cut(s) 42, 432, 754
BseMI GCAATG 1 cut(s) 896
BseMII CTCAG 3 cut(s) 264, 1428, 1601
BseNI ACTGG 2 cut(s) 685, 1656
BseSI GKGCMC 2 cut(s) 121, 1129
BseXI GCAGC 6 cut(s) 314, 676, 862, 869, 1233, 1520
BsgI GTGCAG 1 cut(s) 737
BshFI GGCC 4 cut(s) 77, 234, 747, 1413
BshNI GGYRCC 1 cut(s) 374
BsiHKAI GWGCWC 3 cut(s) 678, 860, 1129
BsiHKCI CYCGRG 2 cut(s) 479, 914
BslFI GGGAC 1 cut(s) 1607
BslI CCNNNNNNNGG 3 cut(s) 42, 432, 754
BsmAI GTCTC 3 cut(s) 180, 482, 1171
BsmBI CGTCTC 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 1607
BsmI GAATGC 3 cut(s) 464, 607, 988
BsnI GGCC 4 cut(s) 77, 234, 747, 1413
Bso31I GGTCTC 1 cut(s) 180
BsoBI CYCGRG 2 cut(s) 479, 914
Bsp1286I GDGCHC 4 cut(s) 121, 678, 860, 1129
Bsp143I GATC 5 cut(s) 100, 700, 1402, 1446, 1652
Bsp19I CCATGG 2 cut(s) 748, 1027
BspACI CCGC 1 cut(s) 755
BspANI GGCC 4 cut(s) 77, 234, 747, 1413
BspCNI CTCAG 3 cut(s) 265, 1429, 1602
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 3 cut(s) 376, 900, 1654
BspMAI CTGCAG 1 cut(s) 1251
BspMI ACCTGC 1 cut(s) 709
BspPI GGATC 3 cut(s) 1410, 1647, 1660
BspT107I GGYRCC 1 cut(s) 374
BspTNI GGTCTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 896
BsrI ACTGG 2 cut(s) 685, 1656
BssECI CCNNGG 5 cut(s) 748, 777, 1027, 1306, 1372
BssMI GATC 5 cut(s) 100, 700, 1402, 1446, 1652
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 3 cut(s) 748, 1027, 1306
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 5 cut(s) 416, 779, 1374, 1470, 1650
Bst4CI ACNGT 2 cut(s) 258, 523
Bst6I CTCTTC 1 cut(s) 170
BstAPI GCANNNNNTGC 2 cut(s) 1492, 1517
BstBAI YACGTR 1 cut(s) 110
BstC8I GCNNGC 7 cut(s) 421, 616, 880, 887, 1393, 1504, 1518
BstDEI CTNAG 3 cut(s) 273, 1437, 1610
BstDSI CCRYGG 2 cut(s) 748, 1027
BstF5I GGATG 7 cut(s) 376, 820, 1118, 1126, 1334, 1611, 1639
BstHHI GCGC 3 cut(s) 301, 1196, 1508
BstKTI GATC 5 cut(s) 103, 703, 1405, 1449, 1655
BstMAI GTCTC 3 cut(s) 180, 482, 1171
BstMBI GATC 5 cut(s) 100, 700, 1402, 1446, 1652
BstMWI GCNNNNNNNGC 8 cut(s) 187, 611, 983, 998, 1217, 1492, 1508, 1517
BstNI CCWGG 5 cut(s) 416, 779, 1374, 1470, 1650
BstNSI RCATGY 8 cut(s) 117, 220, 423, 1099, 1302, 1395, 1459, 1520
BstSCI CCNGG 5 cut(s) 414, 777, 1372, 1468, 1648
BstSFI CTRYAG 2 cut(s) 1161, 1247
BstSLI GKGCMC 2 cut(s) 121, 1129
BstSNI TACGTA 1 cut(s) 110
BstV1I GCAGC 6 cut(s) 314, 676, 862, 869, 1233, 1520
BstV2I GAAGAC 1 cut(s) 1386
BstX2I RGATCY 2 cut(s) 1402, 1652
BstYI RGATCY 2 cut(s) 1402, 1652
BsuI GTATCC 1 cut(s) 939
BsuRI GGCC 4 cut(s) 77, 234, 747, 1413
BtgI CCRYGG 2 cut(s) 748, 1027
BtsCI GGATG 7 cut(s) 376, 820, 1118, 1126, 1334, 1611, 1639
BtsIMutI CAGTG 2 cut(s) 678, 696
BveI ACCTGC 1 cut(s) 709
Cac8I GCNNGC 7 cut(s) 421, 616, 880, 887, 1393, 1504, 1518
CciI TCATGA 1 cut(s) 832
CfoI GCGC 3 cut(s) 301, 1196, 1508
Cfr13I GGNCC 2 cut(s) 721, 1173
CseI GACGC 1 cut(s) 1588
CsiI ACCWGGT 1 cut(s) 1468
Csp6I GTAC 3 cut(s) 111, 1056, 1336
CviQI GTAC 3 cut(s) 111, 1056, 1336
DdeI CTNAG 3 cut(s) 273, 1437, 1610
DpnI GATC 5 cut(s) 102, 702, 1404, 1448, 1654
DpnII GATC 5 cut(s) 100, 700, 1402, 1446, 1652
DraI TTTAAA 2 cut(s) 846, 1464
DraIII CACNNNGTG 1 cut(s) 1133
EaeI YGGCCR 3 cut(s) 75, 232, 745
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
EciI GGCGGA 1 cut(s) 770
Eco105I TACGTA 1 cut(s) 110
Eco130I CCWWGG 3 cut(s) 748, 1027, 1306
Eco31I GGTCTC 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 721, 1173
Eco88I CYCGRG 2 cut(s) 479, 914
EcoRI GAATTC 1 cut(s) 772
EcoRII CCWGG 5 cut(s) 414, 777, 1372, 1468, 1648
EcoT14I CCWWGG 3 cut(s) 748, 1027, 1306
EcoT22I ATGCAT 6 cut(s) 514, 1118, 1300, 1393, 1397, 1522
ErhI CCWWGG 3 cut(s) 748, 1027, 1306
Esp3I CGTCTC 1 cut(s) 482
FaqI GGGAC 1 cut(s) 1607
FbaI TGATCA 1 cut(s) 1446
Fnu4HI GCNGC 6 cut(s) 328, 690, 876, 883, 1247, 1509
FokI GGATG 7 cut(s) 383, 827, 1125, 1133, 1341, 1618, 1646
Fsp4HI GCNGC 6 cut(s) 328, 690, 876, 883, 1247, 1509
FspAI RTGCGCAY 1 cut(s) 1195
FspBI CTAG 2 cut(s) 1409, 1629
FspI TGCGCA 2 cut(s) 1195, 1507
GlaI GCGC 3 cut(s) 300, 1195, 1507
GluI GCNGC 6 cut(s) 328, 690, 876, 883, 1247, 1509
GsuI CTGGAG 2 cut(s) 398, 1233
HaeIII GGCC 4 cut(s) 77, 234, 747, 1413
HgaI GACGC 1 cut(s) 1588
HhaI GCGC 3 cut(s) 301, 1196, 1508
Hin6I GCGC 3 cut(s) 299, 1194, 1506
HinP1I GCGC 3 cut(s) 299, 1194, 1506
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HindIII AAGCTT 3 cut(s) 447, 825, 1357
HinfI GANTC 7 cut(s) 436, 442, 1142, 1343, 1351, 1365, 1625
HphI GGTGA 3 cut(s) 551, 1267, 1360
Hpy166II GTNNAC 7 cut(s) 396, 460, 519, 542, 963, 1066, 1127
Hpy188I TCNGA 3 cut(s) 100, 441, 640
Hpy188III TCNNGA 5 cut(s) 29, 833, 1223, 1450, 1603
Hpy8I GTNNAC 7 cut(s) 396, 460, 519, 542, 963, 1066, 1127
Hpy99I CGWCG 2 cut(s) 266, 1604
HpyAV CCTTC 5 cut(s) 206, 1007, 1144, 1201, 1247
HpyCH4III ACNGT 2 cut(s) 258, 523
HpyCH4IV ACGT 2 cut(s) 109, 1423
HpyF10VI GCNNNNNNNGC 8 cut(s) 187, 611, 983, 998, 1217, 1492, 1508, 1517
HpyF3I CTNAG 3 cut(s) 273, 1437, 1610
HpySE526I ACGT 2 cut(s) 109, 1423
HspAI GCGC 3 cut(s) 299, 1194, 1506
Ksp22I TGATCA 1 cut(s) 1446
Kzo9I GATC 5 cut(s) 100, 700, 1402, 1446, 1652
LmnI GCTCC 2 cut(s) 413, 428
Lsp1109I GCAGC 6 cut(s) 314, 676, 862, 869, 1233, 1520
LweI GCATC 3 cut(s) 828, 1103, 1596
MabI ACCWGGT 1 cut(s) 1468
MaeI CTAG 2 cut(s) 1409, 1629
MaeII ACGT 2 cut(s) 109, 1423
MaeIII GTNAC 2 cut(s) 105, 694
MalI GATC 5 cut(s) 102, 702, 1404, 1448, 1654
MboI GATC 5 cut(s) 100, 700, 1402, 1446, 1652
MboII GAAGA 5 cut(s) 157, 946, 1030, 1391, 1397
MfeI CAATTG 1 cut(s) 1588
MflI RGATCY 2 cut(s) 1402, 1652
MhlI GDGCHC 4 cut(s) 121, 678, 860, 1129
MlsI TGGCCA 3 cut(s) 77, 234, 747
MluCI AATT 8 cut(s) 150, 772, 1022, 1038, 1267, 1442, 1588, 1643
MluNI TGGCCA 3 cut(s) 77, 234, 747
MlyI GAGTC 5 cut(s) 436, 1151, 1337, 1345, 1619
MmeI TCCRAC 5 cut(s) 153, 277, 792, 987, 1640
Mox20I TGGCCA 3 cut(s) 77, 234, 747
Mph1103I ATGCAT 6 cut(s) 514, 1118, 1300, 1393, 1397, 1522
MroXI GAANNNNTTC 2 cut(s) 497, 1022
MscI TGGCCA 3 cut(s) 77, 234, 747
MseI TTAA 5 cut(s) 306, 705, 845, 1041, 1463
MslI CAYNNNNRTG 2 cut(s) 369, 380
Msp20I TGGCCA 3 cut(s) 77, 234, 747
MspR9I CCNGG 5 cut(s) 416, 779, 1374, 1470, 1650
MunI CAATTG 1 cut(s) 1588
Mva1269I GAATGC 3 cut(s) 464, 607, 988
MvaI CCWGG 5 cut(s) 416, 779, 1374, 1470, 1650
MwoI GCNNNNNNNGC 8 cut(s) 187, 611, 983, 998, 1217, 1492, 1508, 1517
NcoI CCATGG 2 cut(s) 748, 1027
NdeII GATC 5 cut(s) 100, 700, 1402, 1446, 1652
NlaIV GGNNCC 3 cut(s) 376, 900, 1654
NmuCI GTSAC 1 cut(s) 694
NsbI TGCGCA 2 cut(s) 1195, 1507
NsiI ATGCAT 6 cut(s) 514, 1118, 1300, 1393, 1397, 1522
NspI RCATGY 8 cut(s) 117, 220, 423, 1099, 1302, 1395, 1459, 1520
PaeI GCATGC 3 cut(s) 423, 1395, 1520
PaeR7I CTCGAG 1 cut(s) 914
PagI TCATGA 1 cut(s) 832
PciI ACATGT 3 cut(s) 113, 216, 1095
PctI GAATGC 3 cut(s) 464, 607, 988
PdmI GAANNNNTTC 2 cut(s) 497, 1022
PfeI GAWTC 2 cut(s) 436, 1365
PflFI GACNNNGTC 1 cut(s) 1598
PkrI GCNGC 6 cut(s) 329, 691, 877, 884, 1248, 1510
PleI GAGTC 5 cut(s) 436, 1150, 1337, 1345, 1619
PpsI GAGTC 5 cut(s) 436, 1150, 1337, 1345, 1619
Ppu21I YACGTR 1 cut(s) 110
PscI ACATGT 3 cut(s) 113, 216, 1095
Psp6I CCWGG 5 cut(s) 414, 777, 1372, 1468, 1648
PspGI CCWGG 5 cut(s) 414, 777, 1372, 1468, 1648
PspN4I GGNNCC 3 cut(s) 376, 900, 1654
PspPI GGNCC 2 cut(s) 721, 1173
PspXI VCTCGAGB 1 cut(s) 914
PstI CTGCAG 1 cut(s) 1251
PsuI RGATCY 2 cut(s) 1402, 1652
PsyI GACNNNGTC 1 cut(s) 1598
RsaI GTAC 3 cut(s) 112, 1057, 1337
RsaNI GTAC 3 cut(s) 111, 1056, 1336
RseI CAYNNNNRTG 2 cut(s) 369, 380
SaqAI TTAA 5 cut(s) 306, 705, 845, 1041, 1463
SatI GCNGC 6 cut(s) 328, 690, 876, 883, 1247, 1509
Sau3AI GATC 5 cut(s) 100, 700, 1402, 1446, 1652
Sau96I GGNCC 2 cut(s) 721, 1173
ScaI AGTACT 1 cut(s) 1337
SchI GAGTC 5 cut(s) 436, 1151, 1337, 1345, 1619
ScrFI CCNGG 5 cut(s) 416, 779, 1374, 1470, 1650
SduI GDGCHC 4 cut(s) 121, 678, 860, 1129
SexAI ACCWGGT 1 cut(s) 1468
SfaNI GCATC 3 cut(s) 828, 1103, 1596
SfcI CTRYAG 2 cut(s) 1161, 1247
Sfr274I CTCGAG 1 cut(s) 914
SinI GGWCC 2 cut(s) 721, 1173
SlaI CTCGAG 1 cut(s) 914
SmiMI CAYNNNNRTG 2 cut(s) 369, 380
SmlI CTYRAG 4 cut(s) 10, 444, 914, 1223
SmoI CTYRAG 4 cut(s) 10, 444, 914, 1223
SnaBI TACGTA 1 cut(s) 110
SphI GCATGC 3 cut(s) 423, 1395, 1520
Sse9I AATT 8 cut(s) 150, 772, 1022, 1038, 1267, 1442, 1588, 1643
SsiI CCGC 1 cut(s) 755
SspMI CTAG 2 cut(s) 1409, 1629
StyD4I CCNGG 5 cut(s) 414, 777, 1372, 1468, 1648
StyI CCWWGG 3 cut(s) 748, 1027, 1306
TaaI ACNGT 2 cut(s) 258, 523
TaiI ACGT 2 cut(s) 112, 1426
TaqI TCGA 4 cut(s) 261, 486, 915, 1145
TasI AATT 8 cut(s) 150, 772, 1022, 1038, 1267, 1442, 1588, 1643
TatI WGTACW 2 cut(s) 1055, 1335
TfiI GAWTC 2 cut(s) 436, 1365
Tru1I TTAA 5 cut(s) 306, 705, 845, 1041, 1463
Tru9I TTAA 5 cut(s) 306, 705, 845, 1041, 1463
TscAI CASTG 2 cut(s) 685, 703
TseFI GTSAC 1 cut(s) 694
TseI GCWGC 6 cut(s) 327, 689, 875, 882, 1246, 1508
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 6 cut(s) 143, 351, 457, 821, 947, 1163
TspGWI ACGGA 1 cut(s) 1144
TspRI CASTG 2 cut(s) 685, 703
Tth111I GACNNNGTC 1 cut(s) 1598
VneI GTGCAC 1 cut(s) 1125
VpaK11BI GGWCC 2 cut(s) 721, 1173
XapI RAATTY 2 cut(s) 772, 1022
XceI RCATGY 8 cut(s) 117, 220, 423, 1099, 1302, 1395, 1459, 1520
XcmI CCANNNNNNNNNTGG 2 cut(s) 70, 1183
XhoI CTCGAG 1 cut(s) 914
XmnI GAANNNNTTC 2 cut(s) 497, 1022
XspI CTAG 2 cut(s) 1409, 1629
ZrmI AGTACT 1 cut(s) 1337
Zsp2I ATGCAT 6 cut(s) 514, 1118, 1300, 1393, 1397, 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.