Rw5G018150

Pentatricopeptide repeat-containing protein At3g14580

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016716
Physical Location & Seq
Forward (+)
23456044 .. 23457220
1177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G018150.1

Sequence Viewer

Length: 1143 bp
ATGATTTCACGTTCTACCAAATTTCTCACACACCCAATCCTTCAATCGCTAACCCAAACCCCATCTTTCTCCACACTCCCATCTTCCTTCTCCCAAATCCCAAAAAAGCTCCACCATAAAGACTGGCTATCCCCAAACGAAGTTCTCCAAGTCTTCACAGCCCTCCAAGACCCCAACTCAGTCCTCACAGCCCTCGACCACTACTCCAAGCGCAAGGACTACAAGCCCAATGAAGCCCTCTACACCTTGATCATCGACAAGCTCGCCCGAGCCCACCTCTTCGACGCCGTGGACAATGTCATGAACAGAATCAAATCCGAAAGGAAATGCCGGCTATCCGATGACTTCTTCCGCGGTGTTATCAAGAACTACGGCAATGTGGGTGGTCATATCAAGAAGGCGATACAGACCCTTTACGATATGCCCAGTTTCGGGTGCTGGCCGAGTGTGAAGACCTTCAATTTAGTTCTGCATTTGCTGGTCTCGACCAAGATGTTTGATGTTGTTCATCAGGTTTATTTGATGTCGCCGCAATTGGGTGTCGAGATTGATGCTTGTTGTTTGAATATAATCATCAAAGGGCTGTGCGAAAGTGGGAAGCTGGATAGCGCACTCCAGGTGCTCGACGAATTTCCCCAGCAGAAATGTGAGCCGAATGCGCTGACGTTTTCGACGCTGATGCATGGGCTCTGTGTCAATGGGAAAGTGGATGAGGCTTTTGGCCGGATTCCGGTCACGTTTAATATATTGATAGCAGGGCTTAGGAAACAGAAGAGATATGATGAGGGCATTGAGCTTTTGGAGCAAATGAAGCTCAAGGGGTGTGCTCCAAATCCAGCTTCCTATCAGGAGGTTTTGTATTGTTTGCTTGATGCACAGAGCTTCGCTGAGGCGAAACAGTTTATGATTCGGATGGTTTCAAAGCGTGTTGGTCCGAGTTTCGTGTCGTACAAGCAACTGATTCAGGGGTTGTGTAAAGAAAATAGGGTGGAGGATCTGGATTGGGTTTTGAGGCAGATGATGCGACAAGGTTTTGTGCCAAAGATGGGTATGTGGAGGCAGATTATCAGAAGTGTGTTTTCAAAGAAGAGCAACCATTATTGTGTTTCTTGTGAAGAGACTATAGATGATTTGATCACTTGA

Protein Analysis

380

Amino Acids

43.36

Weight (kDa)

8.88

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 179 - 228 1e-05 Pentatricopeptide repeat domain
PPR_1 PF12854 180 - 210 8e-08 PPR repeat
PPR_2 PF13041 184 - 231 7.3e-13 PPR repeat family
PPR_1 PF12854 216 - 240 2.2e-08 PPR repeat
PPR_2 PF13041 245 - 283 5.8e-09 PPR repeat family
PPR PF01535 245 - 275 4.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016716)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14580
fragaria_vesca FvH4_3g16970
malus_domestica MD11G1276100.v1.1
prunus_persica Prupe.4G152100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0028281
rosa_laevigata RLG00000033079
rosa_multiflora Rmu_sc0001571.1_g000002
rosa_roxburghii Rroxscaffold_1G00051440
rosa_rugosa Rorug05G0105500
rosa_samantha Rh5AG199000 Rh5BG197500 Rh5CG217300 Rh5DG199200
rosa_wichuraiana Rw5G018150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 354
AciI CCGC 3 cut(s) 352, 354, 530
AclWI GGATC 1 cut(s) 1002
AcoI YGGCCR 2 cut(s) 440, 721
AcsI RAATTY 2 cut(s) 20, 629
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 1 cut(s) 950
AfiI CCNNNNNNNGG 5 cut(s) 431, 432, 536, 730, 1046
AgsI TTSAA 5 cut(s) 44, 460, 565, 921, 1083
AjnI CCWGG 1 cut(s) 615
AjuI GAANNNNNNNTTGG 2 cut(s) 823, 855
AluBI AGCT 7 cut(s) 109, 262, 601, 796, 814, 839, 882
AluI AGCT 7 cut(s) 109, 262, 601, 796, 814, 839, 882
Alw21I GWGCWC 2 cut(s) 624, 829
Alw26I GTCTC 2 cut(s) 487, 1112
AlwI GGATC 1 cut(s) 1002
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 2 cut(s) 440, 721
ApoI RAATTY 2 cut(s) 20, 629
Asp700I GAANNNNTTC 1 cut(s) 455
AspLEI GCGC 3 cut(s) 213, 611, 661
AspS9I GGNCC 1 cut(s) 932
AvaI CYCGRG 1 cut(s) 267
AvaII GGWCC 1 cut(s) 932
BanII GRGCYC 2 cut(s) 274, 690
BbsI GAAGAC 2 cut(s) 145, 458
Bbv12I GWGCWC 2 cut(s) 624, 829
BbvCI CCTCAGC 1 cut(s) 888
BccI CCATC 4 cut(s) 70, 88, 907, 1039
BceAI ACGGC 2 cut(s) 272, 388
BcgI CGANNNNNNTGC 4 cut(s) 533, 567, 661, 695
BciT130I CCWGG 1 cut(s) 617
BclI TGATCA 2 cut(s) 249, 1134
BcoDI GTCTC 2 cut(s) 487, 1112
BfmI CTRYAG 1 cut(s) 1122
BisI GCNGC 1 cut(s) 530
BlsI GCNGC 1 cut(s) 531
Bme1390I CCNGG 1 cut(s) 617
Bme18I GGWCC 1 cut(s) 932
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 1 cut(s) 932
BmrFI CCNGG 1 cut(s) 617
BmrI ACTGGG 1 cut(s) 420
BmsI GCATC 4 cut(s) 541, 669, 862, 1011
BmuI ACTGGG 1 cut(s) 420
BpiI GAAGAC 2 cut(s) 145, 458
BplI GAGNNNNNCTC 2 cut(s) 261, 293
BpmI CTGGAG 1 cut(s) 599
Bpu10I CCTNAGC 2 cut(s) 761, 888
BpuEI CTTGAG 1 cut(s) 800
BsaHI GRCGYC 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 2 cut(s) 288, 352
BsaWI WCCGGW 1 cut(s) 730
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 842, 872
Bsc4I CCNNNNNNNGG 5 cut(s) 431, 432, 536, 730, 1046
Bse118I RCCGGY 1 cut(s) 330
Bse1I ACTGG 2 cut(s) 128, 426
Bse3DI GCAATG 1 cut(s) 382
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 2 cut(s) 288, 352
BseGI GGATG 2 cut(s) 715, 918
BseLI CCNNNNNNNGG 5 cut(s) 431, 432, 536, 730, 1046
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 2 cut(s) 192, 879
BseNI ACTGG 2 cut(s) 128, 426
BseYI CCCAGC 1 cut(s) 636
Bsh1236I CGCG 1 cut(s) 354
BshFI GGCC 2 cut(s) 442, 723
BsiHKAI GWGCWC 2 cut(s) 624, 829
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 3 cut(s) 331, 724, 731
BslI CCNNNNNNNGG 5 cut(s) 431, 432, 536, 730, 1046
BsmAI GTCTC 2 cut(s) 487, 1112
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 2 cut(s) 442, 723
Bso31I GGTCTC 1 cut(s) 487
BsoBI CYCGRG 1 cut(s) 267
Bsp1286I GDGCHC 4 cut(s) 274, 624, 690, 829
Bsp143I GATC 3 cut(s) 249, 994, 1134
BspACI CCGC 3 cut(s) 352, 354, 530
BspANI GGCC 2 cut(s) 442, 723
BspCNI CTCAG 2 cut(s) 191, 880
BspFNI CGCG 1 cut(s) 354
BspHI TCATGA 1 cut(s) 300
BspPI GGATC 1 cut(s) 1002
BspQI GCTCTTC 1 cut(s) 1082
BspTNI GGTCTC 1 cut(s) 487
BsrDI GCAATG 1 cut(s) 382
BsrFI RCCGGY 1 cut(s) 330
BsrI ACTGG 2 cut(s) 128, 426
BssAI RCCGGY 1 cut(s) 330
BssECI CCNNGG 2 cut(s) 288, 352
BssMI GATC 3 cut(s) 249, 994, 1134
BssNI GRCGYC 1 cut(s) 285
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 1 cut(s) 900
Bst6I CTCTTC 4 cut(s) 284, 767, 1082, 1110
BstACI GRCGYC 1 cut(s) 285
BstAPI GCANNNNNTGC 1 cut(s) 1021
BstC8I GCNNGC 3 cut(s) 264, 332, 440
BstDEI CTNAG 3 cut(s) 178, 761, 888
BstDSI CCRYGG 2 cut(s) 288, 352
BstF5I GGATG 2 cut(s) 715, 918
BstFNI CGCG 1 cut(s) 354
BstHHI GCGC 3 cut(s) 213, 611, 661
BstKTI GATC 3 cut(s) 252, 997, 1137
BstMAI GTCTC 2 cut(s) 487, 1112
BstMBI GATC 3 cut(s) 249, 994, 1134
BstMWI GCNNNNNNNGC 4 cut(s) 658, 802, 811, 1021
BstNI CCWGG 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 615
BstSFI CTRYAG 1 cut(s) 1122
BstUI CGCG 1 cut(s) 354
BstV2I GAAGAC 2 cut(s) 145, 458
BstX2I RGATCY 1 cut(s) 994
BstYI RGATCY 1 cut(s) 994
BsuRI GGCC 2 cut(s) 442, 723
BtgI CCRYGG 2 cut(s) 288, 352
BtsCI GGATG 2 cut(s) 715, 918
Cac8I GCNNGC 3 cut(s) 264, 332, 440
CciI TCATGA 1 cut(s) 300
CfoI GCGC 3 cut(s) 213, 611, 661
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 1 cut(s) 932
Cfr42I CCGCGG 1 cut(s) 355
CseI GACGC 2 cut(s) 293, 682
Csp6I GTAC 1 cut(s) 949
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviAII CATG 2 cut(s) 301, 683
CviQI GTAC 1 cut(s) 949
DdeI CTNAG 3 cut(s) 178, 761, 888
DpnI GATC 3 cut(s) 251, 996, 1136
DpnII GATC 3 cut(s) 249, 994, 1134
EaeI YGGCCR 2 cut(s) 440, 721
Eam1104I CTCTTC 4 cut(s) 284, 767, 1082, 1110
EarI CTCTTC 4 cut(s) 284, 767, 1082, 1110
Eco24I GRGCYC 2 cut(s) 274, 690
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 932
Eco88I CYCGRG 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 615
EcoT22I ATGCAT 1 cut(s) 684
EcoT38I GRGCYC 2 cut(s) 274, 690
FaeI CATG 2 cut(s) 304, 686
FatI CATG 2 cut(s) 300, 682
FbaI TGATCA 2 cut(s) 249, 1134
Fnu4HI GCNGC 1 cut(s) 530
FokI GGATG 2 cut(s) 722, 925
FriOI GRGCYC 2 cut(s) 274, 690
Fsp4HI GCNGC 1 cut(s) 530
GlaI GCGC 3 cut(s) 212, 610, 660
GluI GCNGC 1 cut(s) 530
GsaI CCCAGC 1 cut(s) 640
GsuI CTGGAG 1 cut(s) 599
HaeIII GGCC 2 cut(s) 442, 723
HapII CCGG 3 cut(s) 331, 724, 731
HgaI GACGC 2 cut(s) 293, 682
HhaI GCGC 3 cut(s) 213, 611, 661
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 2 cut(s) 304, 686
Hin6I GCGC 3 cut(s) 211, 609, 659
HinP1I GCGC 3 cut(s) 211, 609, 659
HinfI GANTC 4 cut(s) 309, 727, 907, 961
HpaII CCGG 3 cut(s) 331, 724, 731
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 5 cut(s) 319, 340, 912, 936, 1070
Hpy188III TCNNGA 7 cut(s) 301, 364, 394, 484, 544, 848, 998
Hpy8I GTNNAC 1 cut(s) 292
Hpy99I CGWCG 3 cut(s) 287, 629, 676
HpyAV CCTTC 4 cut(s) 50, 97, 391, 466
HpyCH4III ACNGT 1 cut(s) 900
HpyCH4IV ACGT 3 cut(s) 10, 665, 737
HpyCH4V TGCA 3 cut(s) 472, 682, 875
HpyF10VI GCNNNNNNNGC 4 cut(s) 658, 802, 811, 1021
HpyF3I CTNAG 3 cut(s) 178, 761, 888
HpySE526I ACGT 3 cut(s) 10, 665, 737
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 2 cut(s) 304, 686
HspAI GCGC 3 cut(s) 211, 609, 659
KroI GCCGGC 1 cut(s) 330
KroNI GCCGGC 1 cut(s) 332
Ksp22I TGATCA 2 cut(s) 249, 1134
KspI CCGCGG 1 cut(s) 355
Kzo9I GATC 3 cut(s) 249, 994, 1134
LguI GCTCTTC 1 cut(s) 1082
LmnI GCTCC 3 cut(s) 114, 802, 832
LweI GCATC 4 cut(s) 541, 669, 862, 1011
MaeII ACGT 3 cut(s) 10, 665, 737
MaeIII GTNAC 1 cut(s) 733
MalI GATC 3 cut(s) 251, 996, 1136
MboI GATC 3 cut(s) 249, 994, 1134
MboII GAAGA 8 cut(s) 75, 145, 271, 340, 463, 784, 1099, 1127
MfeI CAATTG 1 cut(s) 533
MflI RGATCY 1 cut(s) 994
MhlI GDGCHC 4 cut(s) 274, 624, 690, 829
MluCI AATT 4 cut(s) 20, 460, 533, 629
Mph1103I ATGCAT 1 cut(s) 684
MroNI GCCGGC 1 cut(s) 330
MroXI GAANNNNTTC 1 cut(s) 455
MseI TTAA 1 cut(s) 741
MslI CAYNNNNRTG 1 cut(s) 1101
MspA1I CMGCKG 1 cut(s) 354
MspI CCGG 3 cut(s) 331, 724, 731
MspR9I CCNGG 1 cut(s) 617
MunI CAATTG 1 cut(s) 533
Mva1269I GAATGC 1 cut(s) 661
MvaI CCWGG 1 cut(s) 617
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 4 cut(s) 658, 802, 811, 1021
NaeI GCCGGC 1 cut(s) 332
NdeII GATC 3 cut(s) 249, 994, 1134
NgoMIV GCCGGC 1 cut(s) 330
NlaIII CATG 2 cut(s) 304, 686
NmeAIII GCCGAG 1 cut(s) 468
NmuCI GTSAC 1 cut(s) 733
NsiI ATGCAT 1 cut(s) 684
PagI TCATGA 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 1082
PctI GAATGC 1 cut(s) 661
PdiI GCCGGC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 455
PfeI GAWTC 4 cut(s) 309, 727, 907, 961
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 1 cut(s) 531
Psp6I CCWGG 1 cut(s) 615
PspFI CCCAGC 1 cut(s) 636
PspGI CCWGG 1 cut(s) 615
PspPI GGNCC 1 cut(s) 932
PsuI RGATCY 1 cut(s) 994
PsyI GACNNNGTC 1 cut(s) 296
RsaI GTAC 1 cut(s) 950
RsaNI GTAC 1 cut(s) 949
RseI CAYNNNNRTG 1 cut(s) 1101
SacII CCGCGG 1 cut(s) 355
SapI GCTCTTC 1 cut(s) 1082
SaqAI TTAA 1 cut(s) 741
SatI GCNGC 1 cut(s) 530
Sau3AI GATC 3 cut(s) 249, 994, 1134
Sau96I GGNCC 1 cut(s) 932
ScrFI CCNGG 1 cut(s) 617
SduI GDGCHC 4 cut(s) 274, 624, 690, 829
SfaNI GCATC 4 cut(s) 541, 669, 862, 1011
SfcI CTRYAG 1 cut(s) 1122
Sfr303I CCGCGG 1 cut(s) 355
SgrBI CCGCGG 1 cut(s) 355
SinI GGWCC 1 cut(s) 932
SmiMI CAYNNNNRTG 1 cut(s) 1101
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
Sse9I AATT 4 cut(s) 20, 460, 533, 629
SsiI CCGC 3 cut(s) 352, 354, 530
StyD4I CCNGG 1 cut(s) 615
TaaI ACNGT 1 cut(s) 900
TaiI ACGT 3 cut(s) 13, 668, 740
TaqI TCGA 7 cut(s) 195, 255, 282, 485, 543, 624, 671
TasI AATT 4 cut(s) 20, 460, 533, 629
TauI GCSGC 1 cut(s) 532
TfiI GAWTC 4 cut(s) 309, 727, 907, 961
Tru1I TTAA 1 cut(s) 741
Tru9I TTAA 1 cut(s) 741
TseFI GTSAC 1 cut(s) 733
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 4 cut(s) 246, 317, 497, 824
Tth111I GACNNNGTC 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 932
XapI RAATTY 2 cut(s) 20, 629
XmnI GAANNNNTTC 1 cut(s) 455
Zsp2I ATGCAT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.