Rw5G033510

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0019416
Physical Location & Seq
Reverse (-)
57035874 .. 57037570
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033510.1

Sequence Viewer

Length: 1044 bp
ATGTCTCAGAAAAGCGAATCCTCGTTCTGTGCAAAGAATTCTGCTATCGAGAATGGTACTCAACTGTTATTGGTCCATGGTACTACTGTTACCTTCGAGGTCGAAGGGGAAACCGTTTCCCGTGTATTAGGAAATGAAGTTTACTCCTTGCACTATGATGACCCCACTTTTCTTGAGCACAGCAGGATTCGAGTCCCAGTTGGTCCTAGGTGGAATATATGTTTTCGTGTTGCTGGCATTTGTGATGGCATAGTTTTGCTTGCAGATGATGTCCGTATATATGGTTACAACTTCATATTATGCAATCCATTCCTAAGAAAGTCGGTTATCCTTCCCCATCCTAATGTTACCTTCGAGACTCATGGTCTCTATGAAGCTTCTGTTGGGTTTGGTCATGATGCTATGAACAATGACTATAAGGTTGTGATACTTGCCAGTCTTTTGGATGAATTACCGGGTAGTGAATGTCGAACTGTTGTTGAGCTTTATTCACTTGTTGCACACTCTTGGAAGCACCCTCAAATTGCTCCTCTAATTAGAATTGAGGGACGTGCACGCGCTGCTTTTGTTGATGGTGCTCTTCATTGGCCAGCATCGCGGAGGGAAAATAACAATCATTACAATTTTATTTTGGTATTTGATGTCAGCACTGAGTCATTTCGCCCAGAGATAATGATGCCAAAGAGTGTAAGAACACTTGTGGAAGTTGAGTCATTGGAATTGTCTCTTTCGAGTGATCAGAAATCCATTGCTTTATTTGTTGAAGACGCTGGTGTTCTTCAGGTTTGGGTATTGAATGATTATGGCAGGCCTGAGTCCTGGACCCTATTGATGCAAGTAGCATCATTTGCTTCATTATGTTTCATGAGAAGTGGTGAATTAATACTTGCTGATGATCTGGAAGGGTACTATGGATTACTTTCACAAAAACCTGTGACCCGAGAACGTAGAAGACTTGAGGTTACTGGATATGAGGACTACACTGTGGATTCTTATGGTCAGACCCTCCAATTACTCAACGAGGAGAATGCAAGATCCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

347

Amino Acids

39.21

Weight (kDa)

5.12

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 78 - 281 1.8e-06 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 80 - 287 9.3e-21 F-box associated beta propeller domain
FBA_3 PF08268 94 - 326 2.8e-15 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019416)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0054431
rosa_laevigata RLG00000034931
rosa_multiflora Rmu_sc0005705.1_g000018
rosa_roxburghii Rroxscaffold_1G00026480
rosa_samantha Rh5CG391700 Rh5DG382500
rosa_wichuraiana Rw5G033510 Rw5G033680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 558, 598
AciI CCGC 1 cut(s) 598
AclWI GGATC 1 cut(s) 1029
AcoI YGGCCR 1 cut(s) 587
AcsI RAATTY 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 764
AfaI GTAC 3 cut(s) 58, 82, 908
AgsI TTSAA 2 cut(s) 764, 796
AhdI GACNNNNNGTC 1 cut(s) 363
AjiI CACGTC 1 cut(s) 551
AjnI CCWGG 1 cut(s) 818
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AluBI AGCT 2 cut(s) 377, 484
AluI AGCT 2 cut(s) 377, 484
Alw21I GWGCWC 3 cut(s) 180, 556, 580
Alw26I GTCTC 4 cut(s) 9, 350, 371, 729
Alw44I GTGCAC 1 cut(s) 552
AlwI GGATC 1 cut(s) 1029
Ama87I CYCGRG 1 cut(s) 939
AoxI GGCC 2 cut(s) 587, 809
ApaLI GTGCAC 1 cut(s) 552
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 1 cut(s) 37
AseI ATTAAT 1 cut(s) 881
AspA2I CCTAGG 1 cut(s) 206
AspLEI GCGC 1 cut(s) 560
AspS9I GGNCC 3 cut(s) 73, 203, 822
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 1 cut(s) 887
AvaI CYCGRG 1 cut(s) 939
AvaII GGWCC 3 cut(s) 73, 203, 822
AvrII CCTAGG 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 556
BaeI ACNNNNGTAYC 2 cut(s) 72, 105
BalI TGGCCA 1 cut(s) 589
BbsI GAAGAC 2 cut(s) 771, 958
Bbv12I GWGCWC 3 cut(s) 180, 556, 580
BbvI GCAGC 1 cut(s) 547
BccI CCATC 3 cut(s) 239, 345, 566
BcgI CGANNNNNNTGC 2 cut(s) 1010, 1044
BciT130I CCWGG 1 cut(s) 820
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 1 cut(s) 456
BcoDI GTCTC 4 cut(s) 9, 350, 371, 729
BfaI CTAG 1 cut(s) 207
BisI GCNGC 1 cut(s) 561
BlnI CCTAGG 1 cut(s) 206
BlsI GCNGC 1 cut(s) 562
Bme1390I CCNGG 2 cut(s) 456, 820
Bme18I GGWCC 3 cut(s) 73, 203, 822
BmeRI GACNNNNNGTC 1 cut(s) 363
BmeT110I CYCGRG 1 cut(s) 939
BmgBI CACGTC 1 cut(s) 551
BmgT120I GGNCC 3 cut(s) 73, 203, 822
BmiI GGNNCC 1 cut(s) 824
BmrFI CCNGG 2 cut(s) 456, 820
BmrI ACTGGG 1 cut(s) 191
BmsI GCATC 5 cut(s) 388, 602, 666, 822, 851
BmuI ACTGGG 1 cut(s) 191
BpiI GAAGAC 2 cut(s) 771, 958
BpuEI CTTGAG 2 cut(s) 194, 977
BpuMI CCSGG 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 76, 206
Bse1I ACTGG 3 cut(s) 197, 435, 970
Bse3DI GCAATG 1 cut(s) 747
BseBI CCWGG 1 cut(s) 820
BseDI CCNNGG 2 cut(s) 76, 206
BseGI GGATG 2 cut(s) 337, 451
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 3 cut(s) 20, 642, 804
BseNI ACTGG 3 cut(s) 197, 435, 970
BseRI GAGGAG 2 cut(s) 519, 1037
BseSI GKGCMC 1 cut(s) 556
BseXI GCAGC 1 cut(s) 547
Bsh1236I CGCG 2 cut(s) 558, 598
BshFI GGCC 2 cut(s) 589, 811
BsiHKAI GWGCWC 3 cut(s) 180, 556, 580
BsiHKCI CYCGRG 1 cut(s) 939
BsiSI CCGG 1 cut(s) 455
BslFI GGGAC 2 cut(s) 179, 561
BsmAI GTCTC 4 cut(s) 9, 350, 371, 729
BsmFI GGGAC 2 cut(s) 179, 561
BsmI GAATGC 1 cut(s) 1033
BsnI GGCC 2 cut(s) 589, 811
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 939
Bsp1286I GDGCHC 3 cut(s) 180, 556, 580
Bsp143I GATC 3 cut(s) 736, 895, 1034
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 1 cut(s) 598
BspANI GGCC 2 cut(s) 589, 811
BspCNI CTCAG 3 cut(s) 19, 643, 805
BspFNI CGCG 2 cut(s) 558, 598
BspHI TCATGA 2 cut(s) 394, 864
BspLI GGNNCC 1 cut(s) 824
BspPI GGATC 1 cut(s) 1029
BspQI GCTCTTC 1 cut(s) 585
BspTNI GGTCTC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 747
BsrI ACTGG 3 cut(s) 197, 435, 970
BssECI CCNNGG 2 cut(s) 76, 206
BssMI GATC 3 cut(s) 736, 895, 1034
BssT1I CCWWGG 2 cut(s) 76, 206
Bst2UI CCWGG 1 cut(s) 820
Bst4CI ACNGT 5 cut(s) 66, 88, 115, 475, 985
Bst6I CTCTTC 1 cut(s) 585
BstAPI GCANNNNNTGC 2 cut(s) 560, 848
BstC8I GCNNGC 5 cut(s) 235, 261, 556, 591, 809
BstDEI CTNAG 4 cut(s) 6, 314, 651, 813
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 2 cut(s) 337, 451
BstFNI CGCG 2 cut(s) 558, 598
BstHHI GCGC 1 cut(s) 560
BstKTI GATC 3 cut(s) 739, 898, 1037
BstMAI GTCTC 4 cut(s) 9, 350, 371, 729
BstMBI GATC 3 cut(s) 736, 895, 1034
BstMWI GCNNNNNNNGC 3 cut(s) 560, 595, 848
BstNI CCWGG 1 cut(s) 820
BstSCI CCNGG 2 cut(s) 454, 818
BstSLI GKGCMC 1 cut(s) 556
BstUI CGCG 2 cut(s) 558, 598
BstV1I GCAGC 1 cut(s) 547
BstV2I GAAGAC 2 cut(s) 771, 958
BstX2I RGATCY 1 cut(s) 1034
BstXI CCANNNNNNTGG 1 cut(s) 442
BstYI RGATCY 1 cut(s) 1034
BsuRI GGCC 2 cut(s) 589, 811
BtgI CCRYGG 1 cut(s) 76
BtgZI GCGATG 1 cut(s) 579
BtrI CACGTC 1 cut(s) 551
BtsCI GGATG 2 cut(s) 337, 451
BtsIMutI CAGTG 2 cut(s) 648, 981
Cac8I GCNNGC 5 cut(s) 235, 261, 556, 591, 809
CciI TCATGA 2 cut(s) 394, 864
CfoI GCGC 1 cut(s) 560
Cfr13I GGNCC 3 cut(s) 73, 203, 822
CseI GACGC 1 cut(s) 776
Csp6I GTAC 3 cut(s) 57, 81, 907
CspCI CAANNNNNGTGG 2 cut(s) 154, 189
CviAII CATG 4 cut(s) 77, 362, 395, 865
CviJI RGCY 4 cut(s) 377, 484, 589, 811
CviKI_1 RGCY 4 cut(s) 377, 484, 589, 811
CviQI GTAC 3 cut(s) 57, 81, 907
DdeI CTNAG 4 cut(s) 6, 314, 651, 813
DpnI GATC 3 cut(s) 738, 897, 1036
DpnII GATC 3 cut(s) 736, 895, 1034
DriI GACNNNNNGTC 1 cut(s) 363
EaeI YGGCCR 1 cut(s) 587
Eam1104I CTCTTC 1 cut(s) 585
Eam1105I GACNNNNNGTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 585
Eco130I CCWWGG 2 cut(s) 76, 206
Eco147I AGGCCT 1 cut(s) 811
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 3 cut(s) 73, 203, 822
Eco57I CTGAAG 1 cut(s) 764
Eco88I CYCGRG 1 cut(s) 939
EcoRI GAATTC 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 818
EcoT14I CCWWGG 2 cut(s) 76, 206
ErhI CCWWGG 2 cut(s) 76, 206
FaeI CATG 4 cut(s) 80, 365, 398, 868
FaqI GGGAC 2 cut(s) 179, 561
FatI CATG 4 cut(s) 76, 361, 394, 864
FbaI TGATCA 1 cut(s) 736
Fnu4HI GCNGC 1 cut(s) 561
FokI GGATG 2 cut(s) 324, 458
Fsp4HI GCNGC 1 cut(s) 561
FspBI CTAG 1 cut(s) 207
GlaI GCGC 1 cut(s) 559
GluI GCNGC 1 cut(s) 561
HaeIII GGCC 2 cut(s) 589, 811
HapII CCGG 1 cut(s) 455
HgaI GACGC 1 cut(s) 776
HhaI GCGC 1 cut(s) 560
Hin1II CATG 4 cut(s) 80, 365, 398, 868
Hin6I GCGC 1 cut(s) 558
HinP1I GCGC 1 cut(s) 558
HindIII AAGCTT 1 cut(s) 375
HinfI GANTC 8 cut(s) 17, 187, 192, 358, 653, 710, 815, 989
HpaII CCGG 1 cut(s) 455
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 2 cut(s) 142, 554
Hpy188I TCNGA 3 cut(s) 9, 741, 1002
Hpy188III TCNNGA 6 cut(s) 49, 173, 355, 395, 865, 899
Hpy8I GTNNAC 2 cut(s) 142, 554
HpyAV CCTTC 5 cut(s) 98, 103, 341, 361, 896
HpyCH4III ACNGT 5 cut(s) 66, 88, 115, 475, 985
HpyCH4IV ACGT 2 cut(s) 550, 946
HpyCH4V TGCA 8 cut(s) 32, 151, 263, 303, 500, 554, 835, 1031
HpyF10VI GCNNNNNNNGC 3 cut(s) 560, 595, 848
HpyF3I CTNAG 4 cut(s) 6, 314, 651, 813
HpySE526I ACGT 2 cut(s) 550, 946
Hsp92II CATG 4 cut(s) 80, 365, 398, 868
HspAI GCGC 1 cut(s) 558
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 3 cut(s) 736, 895, 1034
LguI GCTCTTC 1 cut(s) 585
LmnI GCTCC 1 cut(s) 532
Lsp1109I GCAGC 1 cut(s) 547
LweI GCATC 5 cut(s) 388, 602, 666, 822, 851
MaeI CTAG 1 cut(s) 207
MaeII ACGT 2 cut(s) 550, 946
MaeIII GTNAC 5 cut(s) 88, 284, 346, 934, 961
MalI GATC 3 cut(s) 738, 897, 1036
MboI GATC 3 cut(s) 736, 895, 1034
MboII GAAGA 4 cut(s) 572, 770, 776, 963
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 3 cut(s) 180, 556, 580
MlsI TGGCCA 1 cut(s) 589
MluCI AATT 9 cut(s) 37, 449, 522, 534, 540, 622, 719, 878, 1010
MluNI TGGCCA 1 cut(s) 589
MlyI GAGTC 5 cut(s) 201, 352, 662, 719, 824
Mox20I TGGCCA 1 cut(s) 589
MscI TGGCCA 1 cut(s) 589
MseI TTAA 1 cut(s) 881
MslI CAYNNNNRTG 2 cut(s) 156, 342
Msp20I TGGCCA 1 cut(s) 589
MspI CCGG 1 cut(s) 455
MspR9I CCNGG 2 cut(s) 456, 820
Mva1269I GAATGC 1 cut(s) 1033
MvaI CCWGG 1 cut(s) 820
MvnI CGCG 2 cut(s) 558, 598
MwoI GCNNNNNNNGC 3 cut(s) 560, 595, 848
NciI CCSGG 1 cut(s) 456
NcoI CCATGG 1 cut(s) 76
NdeII GATC 3 cut(s) 736, 895, 1034
NlaIII CATG 4 cut(s) 80, 365, 398, 868
NlaIV GGNNCC 1 cut(s) 824
NmuCI GTSAC 1 cut(s) 934
PagI TCATGA 2 cut(s) 394, 864
PceI AGGCCT 1 cut(s) 811
PciSI GCTCTTC 1 cut(s) 585
PctI GAATGC 1 cut(s) 1033
PfeI GAWTC 3 cut(s) 17, 187, 989
PfoI TCCNGGA 1 cut(s) 818
PkrI GCNGC 1 cut(s) 562
PleI GAGTC 5 cut(s) 200, 352, 661, 718, 823
PpsI GAGTC 5 cut(s) 200, 352, 661, 718, 823
PshBI ATTAAT 1 cut(s) 881
Psp6I CCWGG 1 cut(s) 818
PspGI CCWGG 1 cut(s) 818
PspN4I GGNNCC 1 cut(s) 824
PspPI GGNCC 3 cut(s) 73, 203, 822
PsuI RGATCY 1 cut(s) 1034
RsaI GTAC 3 cut(s) 58, 82, 908
RsaNI GTAC 3 cut(s) 57, 81, 907
RseI CAYNNNNRTG 2 cut(s) 156, 342
SapI GCTCTTC 1 cut(s) 585
SaqAI TTAA 1 cut(s) 881
SatI GCNGC 1 cut(s) 561
Sau3AI GATC 3 cut(s) 736, 895, 1034
Sau96I GGNCC 3 cut(s) 73, 203, 822
SchI GAGTC 5 cut(s) 201, 352, 662, 719, 824
ScrFI CCNGG 2 cut(s) 456, 820
SduI GDGCHC 3 cut(s) 180, 556, 580
SfaNI GCATC 5 cut(s) 388, 602, 666, 822, 851
SinI GGWCC 3 cut(s) 73, 203, 822
SmiMI CAYNNNNRTG 2 cut(s) 156, 342
SmlI CTYRAG 2 cut(s) 173, 956
SmoI CTYRAG 2 cut(s) 173, 956
Sse9I AATT 9 cut(s) 37, 449, 522, 534, 540, 622, 719, 878, 1010
SseBI AGGCCT 1 cut(s) 811
SsiI CCGC 1 cut(s) 598
SspMI CTAG 1 cut(s) 207
StuI AGGCCT 1 cut(s) 811
StyD4I CCNGG 2 cut(s) 454, 818
StyI CCWWGG 2 cut(s) 76, 206
TaaI ACNGT 5 cut(s) 66, 88, 115, 475, 985
TaiI ACGT 2 cut(s) 553, 949
TaqI TCGA 7 cut(s) 48, 96, 102, 190, 354, 469, 731
TasI AATT 9 cut(s) 37, 449, 522, 534, 540, 622, 719, 878, 1010
TfiI GAWTC 3 cut(s) 17, 187, 989
Tru1I TTAA 1 cut(s) 881
Tru9I TTAA 1 cut(s) 881
TscAI CASTG 2 cut(s) 655, 988
TseFI GTSAC 1 cut(s) 934
TseI GCWGC 1 cut(s) 560
Tsp45I GTSAC 1 cut(s) 934
TspDTI ATGAA 8 cut(s) 150, 283, 387, 419, 462, 572, 843, 853
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 2 cut(s) 655, 988
VneI GTGCAC 1 cut(s) 552
VpaK11BI GGWCC 3 cut(s) 73, 203, 822
VspI ATTAAT 1 cut(s) 881
XapI RAATTY 1 cut(s) 37
XmaJI CCTAGG 1 cut(s) 206
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.