Rw6G022990

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015877
Physical Location & Seq
Forward (+)
45192139 .. 45194854
2716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G022990.1

Sequence Viewer

Length: 642 bp
ATGGCGGACGACGAAGCCGGAGGCGAGGAGTACTTGTTCAAGGTGGTCCTCATCGGCGACTCCGCCGTCGGCAAGTCCAACCTGCTGTCCCGGTTCGCTAGGAACGAGTTCGATCAAAACTCCAAGGCCACAATTGGGGTCGAGTTTCAGACCCAGGCTGTGGAAATCGACGGCAAAGAGGTCAAAGCCCAGATCTGGGACACGGCGGGACAAGAGCGCTTCCGGGCTGTCACGTCTGCTTACTACAGAGGCGCGGTGGGCGCGCTTGTGGTGTATGATATCAGTAGGAAGACTACTTTTGAGAGTGTGGAGCGCTGGCTTAATGAGCTTACCATGCATTGTGATACTGCGGTGGCAAAGATGTTGGTAGGGAATAAGTGTGATTTGGAGGATATTAGAGATGTGTCCATTGAAGATGGTAAAAGCCTTGCAGAAGAAAAGGGACTATTCTTCATGGAGACTTCTGCACTTGATGCTACTAATGTTCAAGAGGCCTTTGAGGTTGTTATCCGGGAGATTTACAACAATGTCAGCAGGAAAGTCCTAAATTCTGATTCTTACAAGGCTGAGCTGTCTGTTAACCGAGTAACCCTGGTGAAAAATGGGGCAGATTCGACGAAATATCGGTGTTGTAGCGGATGA

Protein Analysis

213

Amino Acids

23.66

Weight (kDa)

4.83

Isoelectric Point (pI)

28.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 13 - 172 3.7e-15 ADP-ribosylation factor family
Ras PF00071 14 - 174 5.2e-64 Ras family
Roc PF08477 14 - 128 8.5e-36 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 14 - 111 8e-06 50S ribosome-binding GTPase
GTP_EFTU PF00009 45 - 173 6e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07410
fragaria_vesca FvH4_2g17980
malus_domestica MD05G1163800.v1.1
prunus_persica Prupe.8G192300_v2.0.a1
pyrus_communis pycom05g15300 pycom10g13250
rosa_chinensis RchiOBHm_Chr6g0283281
rosa_laevigata RLG00000012834
rosa_roxburghii Rroxscaffold_7G00185600
rosa_rugosa Rorug06G0152800
rosa_samantha Rh6AG265300 Rh6BG268300 Rh6CG267600 Rh6DG261000
rosa_wichuraiana Rw6G022990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 90
AccB7I CCANNNNNTGG 1 cut(s) 160
AccII CGCG 2 cut(s) 254, 263
AciI CCGC 6 cut(s) 5, 63, 206, 254, 350, 636
AcsI RAATTY 1 cut(s) 547
AfaI GTAC 1 cut(s) 32
AfeI AGCGCT 2 cut(s) 218, 314
AfiI CCNNNNNNNGG 4 cut(s) 135, 160, 195, 196
AgsI TTSAA 3 cut(s) 40, 413, 488
AjiI CACGTC 1 cut(s) 234
AjnI CCWGG 2 cut(s) 153, 591
AloI GAACNNNNNNTCC 2 cut(s) 20, 52
AluBI AGCT 2 cut(s) 328, 571
AluI AGCT 2 cut(s) 328, 571
Alw26I GTCTC 1 cut(s) 452
Aor51HI AGCGCT 2 cut(s) 218, 314
AoxI GGCC 2 cut(s) 126, 492
ApoI RAATTY 1 cut(s) 547
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 5 cut(s) 219, 254, 263, 265, 315
AspS9I GGNCC 1 cut(s) 46
AsuC2I CCSGG 3 cut(s) 91, 224, 512
AsuHPI GGTGA 1 cut(s) 607
AvaII GGWCC 1 cut(s) 46
BaeI ACNNNNGTAYC 2 cut(s) 336, 369
BbsI GAAGAC 1 cut(s) 296
BccI CCATC 1 cut(s) 410
BceAI ACGGC 3 cut(s) 50, 187, 219
BciT130I CCWGG 2 cut(s) 155, 593
BcnI CCSGG 3 cut(s) 91, 224, 512
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 244
BfoI RGCGCY 2 cut(s) 220, 316
BfuAI ACCTGC 1 cut(s) 90
BglII AGATCT 1 cut(s) 192
BlpI GCTNAGC 1 cut(s) 567
BmcAI AGTACT 1 cut(s) 32
Bme1390I CCNGG 5 cut(s) 91, 155, 224, 512, 593
Bme18I GGWCC 1 cut(s) 46
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 46
BmrFI CCNGG 5 cut(s) 91, 155, 224, 512, 593
BmsI GCATC 1 cut(s) 463
BpiI GAAGAC 1 cut(s) 296
Bpu1102I GCTNAGC 1 cut(s) 567
BpuMI CCSGG 3 cut(s) 91, 224, 512
BsaJI CCNNGG 3 cut(s) 123, 153, 591
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 160, 195, 196
BseBI CCWGG 2 cut(s) 155, 593
BseDI CCNNGG 3 cut(s) 123, 153, 591
BseLI CCNNNNNNNGG 4 cut(s) 135, 160, 195, 196
BseMII CTCAG 1 cut(s) 558
BsePI GCGCGC 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 41
BsgI GTGCAG 1 cut(s) 450
Bsh1236I CGCG 2 cut(s) 254, 263
BshFI GGCC 2 cut(s) 128, 494
BsiSI CCGG 4 cut(s) 18, 91, 223, 511
BslFI GGGAC 4 cut(s) 73, 212, 222, 456
BslI CCNNNNNNNGG 4 cut(s) 135, 160, 195, 196
BsmAI GTCTC 1 cut(s) 452
BsmFI GGGAC 4 cut(s) 73, 212, 222, 456
BsnI GGCC 2 cut(s) 128, 494
Bsp143I GATC 2 cut(s) 112, 192
Bsp1720I GCTNAGC 1 cut(s) 567
BspACI CCGC 6 cut(s) 5, 63, 206, 254, 350, 636
BspANI GGCC 2 cut(s) 128, 494
BspCNI CTCAG 1 cut(s) 559
BspFNI CGCG 2 cut(s) 254, 263
BspMI ACCTGC 1 cut(s) 90
BssECI CCNNGG 3 cut(s) 123, 153, 591
BssHII GCGCGC 1 cut(s) 261
BssMI GATC 2 cut(s) 112, 192
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 2 cut(s) 155, 593
BstAPI GCANNNNNTGC 1 cut(s) 473
BstC8I GCNNGC 2 cut(s) 263, 317
BstDEI CTNAG 1 cut(s) 567
BstFNI CGCG 2 cut(s) 254, 263
BstH2I RGCGCY 2 cut(s) 220, 316
BstHHI GCGC 5 cut(s) 219, 254, 263, 265, 315
BstKTI GATC 2 cut(s) 115, 195
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 2 cut(s) 112, 192
BstMWI GCNNNNNNNGC 5 cut(s) 258, 260, 325, 334, 473
BstNI CCWGG 2 cut(s) 155, 593
BstSCI CCNGG 5 cut(s) 89, 153, 222, 510, 591
BstSFI CTRYAG 1 cut(s) 244
BstUI CGCG 2 cut(s) 254, 263
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BsuRI GGCC 2 cut(s) 128, 494
BtrI CACGTC 1 cut(s) 234
BveI ACCTGC 1 cut(s) 90
Cac8I GCNNGC 2 cut(s) 263, 317
CfoI GCGC 5 cut(s) 219, 254, 263, 265, 315
Cfr13I GGNCC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 31
CviAII CATG 2 cut(s) 334, 454
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 567
DpnI GATC 2 cut(s) 114, 194
DpnII GATC 2 cut(s) 112, 192
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
EciI GGCGGA 2 cut(s) 20, 52
Eco130I CCWWGG 1 cut(s) 123
Eco147I AGGCCT 1 cut(s) 494
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 46
Eco47III AGCGCT 2 cut(s) 218, 314
EcoRII CCWGG 2 cut(s) 153, 591
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 2 cut(s) 337, 457
FaiI YATR 3 cut(s) 276, 335, 455
FaqI GGGAC 4 cut(s) 73, 212, 222, 456
FatI CATG 2 cut(s) 333, 453
FauI CCCGC 1 cut(s) 199
FspBI CTAG 1 cut(s) 99
GlaI GCGC 5 cut(s) 218, 253, 262, 264, 314
HaeII RGCGCY 2 cut(s) 220, 316
HaeIII GGCC 2 cut(s) 128, 494
HapII CCGG 4 cut(s) 18, 91, 223, 511
HhaI GCGC 5 cut(s) 219, 254, 263, 265, 315
Hin1II CATG 2 cut(s) 337, 457
Hin6I GCGC 5 cut(s) 217, 252, 261, 263, 313
HinP1I GCGC 5 cut(s) 217, 252, 261, 263, 313
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 3 cut(s) 59, 554, 611
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 4 cut(s) 18, 91, 223, 511
HphI GGTGA 1 cut(s) 607
Hpy166II GTNNAC 1 cut(s) 580
Hpy188I TCNGA 2 cut(s) 150, 553
Hpy188III TCNNGA 1 cut(s) 488
Hpy8I GTNNAC 1 cut(s) 580
Hpy99I CGWCG 4 cut(s) 14, 71, 173, 619
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 3 cut(s) 337, 431, 467
HpyF10VI GCNNNNNNNGC 5 cut(s) 258, 260, 325, 334, 473
HpyF3I CTNAG 1 cut(s) 567
HpySE526I ACGT 1 cut(s) 233
Hsp92II CATG 2 cut(s) 337, 457
HspAI GCGC 5 cut(s) 217, 252, 261, 263, 313
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 2 cut(s) 112, 192
LmnI GCTCC 1 cut(s) 310
LweI GCATC 1 cut(s) 463
MaeI CTAG 1 cut(s) 99
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 2 cut(s) 229, 586
MalI GATC 2 cut(s) 114, 194
MboI GATC 2 cut(s) 112, 192
MboII GAAGA 4 cut(s) 301, 425, 442, 446
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 1 cut(s) 192
MluCI AATT 2 cut(s) 132, 547
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 8 cut(s) 14, 19, 59, 172, 242, 382, 484, 493
Mph1103I ATGCAT 1 cut(s) 339
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 2 cut(s) 321, 579
MspI CCGG 4 cut(s) 18, 91, 223, 511
MspR9I CCNGG 5 cut(s) 91, 155, 224, 512, 593
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 2 cut(s) 155, 593
MvnI CGCG 2 cut(s) 254, 263
MwoI GCNNNNNNNGC 5 cut(s) 258, 260, 325, 334, 473
NciI CCSGG 3 cut(s) 91, 224, 512
NdeII GATC 2 cut(s) 112, 192
NlaIII CATG 2 cut(s) 337, 457
NmuCI GTSAC 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 339
PauI GCGCGC 1 cut(s) 261
PceI AGGCCT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 2 cut(s) 554, 611
PflMI CCANNNNNTGG 1 cut(s) 160
PfoI TCCNGGA 1 cut(s) 510
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
Psp6I CCWGG 2 cut(s) 153, 591
PspGI CCWGG 2 cut(s) 153, 591
PspPI GGNCC 1 cut(s) 46
PsuI RGATCY 1 cut(s) 192
PteI GCGCGC 1 cut(s) 261
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SaqAI TTAA 2 cut(s) 321, 579
Sau3AI GATC 2 cut(s) 112, 192
Sau96I GGNCC 1 cut(s) 46
ScaI AGTACT 1 cut(s) 32
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 5 cut(s) 91, 155, 224, 512, 593
SetI ASST 7 cut(s) 45, 84, 183, 236, 330, 504, 573
SfaNI GCATC 1 cut(s) 463
SfcI CTRYAG 1 cut(s) 244
SinI GGWCC 1 cut(s) 46
Sse9I AATT 2 cut(s) 132, 547
SseBI AGGCCT 1 cut(s) 494
SsiI CCGC 6 cut(s) 5, 63, 206, 254, 350, 636
SspMI CTAG 1 cut(s) 99
StuI AGGCCT 1 cut(s) 494
StyD4I CCNGG 5 cut(s) 89, 153, 222, 510, 591
StyI CCWWGG 1 cut(s) 123
TaiI ACGT 1 cut(s) 236
TaqI TCGA 4 cut(s) 111, 141, 168, 614
TasI AATT 2 cut(s) 132, 547
TatI WGTACW 1 cut(s) 30
TfiI GAWTC 2 cut(s) 554, 611
Tru1I TTAA 2 cut(s) 321, 579
Tru9I TTAA 2 cut(s) 321, 579
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 442
Van91I CCANNNNNTGG 1 cut(s) 160
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 1 cut(s) 547
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 1 cut(s) 99
ZrmI AGTACT 1 cut(s) 32
Zsp2I ATGCAT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.