Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0011099

Physical Location & Seq

Genomic Coordinates Forward (+)

23610478 .. 23616357

Gene Structure 5880 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G020630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGGGAAGTGGAGTCTCCACCCTCTCCTCTTGCTTCAGATCGGCTCACCCAACCCCCGACCACAACGACGTCGTCTTCTCCGCCTCCGAGCCCTTGGACGAAACCCTAGGCCATTCTTTCTGCTATGTAAGGTCCTCCGCTCGCTTCCTCTCCCCCACTCAGTCGGATCGCTTTCTTTCCCCTTCCAACTCCCTCCGGTTCTCGCCGCCGCACGAGGCCCGCGCCCGGCCCGGCCTCCCCGAGACCGGGTTCAAGGCCATCTCCGGCGCCTCCGTCAGTGCCAACAGCTCCACTCCCCGGACTGTCCTCCAGCTTGACAATATTTACGACGATGCCACTGAGAGTGTGAACGGTGGCTTCGGCGGCGGAGTGAGGGGCAGCATCGTCAATGGTTTCGAGAGTACGTCGTCGTTTAGTGCCCTGCCTCTCCAGCCGGTGCCGCGGGGAGGCGAGCCCTCCGGCCCAATGGAGCGCGGCGGCTTCTTTCTCTCCGGTCCGATAGAGCGCGGGGCGCTCTCCGGTCCGCTCGACTCGAACCCGCCCGGACCGGACGGCGATGTCCACTTCTCGGCGCCGCTCGGCGGTCTTTACGCCAAGAAGAGGCGGAAGAAGGGCATTTCCGGGATCCGGAAGGCGTTTTACCGCAACTTCGAGAAGAAGCAACGGCCCTGGGTGGTGCCGGTGCTCAATAATTTCGTCGGCCGGAAGGAGGGGCCACCGGCGAACGGGGAGGAGCACGAGACGGCGAGGAATAATGAGACCAATGTCCAGTGGGCTCTCGGCAAAGCCGGGGAGGACCGGGTACATGTGGTGGTTTCGGAGGAGCAAGGGTGGCTCTTTGTGGGGATTTATGATGGGTTTAACGGCCCTGATGCGCCGGAGTTCTTGATGGGGAATCTTTACCGTGCTTTTTACAATGAGCTTCAGGGCTTGTTTTGGGAGGTTGATGATGATGAGGAAGCCCAAGAAGGCAGTCATGCAAACCCACCAGATGTTGCCCAACAAGTTAATGCTACTACTAGTACTAGCTCACCAAATCTTGCTTCAGAGGGTGAGGCTAAAATTGATGGGGAAGTTTCGGATGCTGGAAATGGGTCCAATTCGAATTCTGACCCCTCGGGGGCAGGTGATCGGACAAAGAGAGTGACATTTCAACCGGAAGGGAGGAGGCGCCGGCTGTGGGAGTTACTCCGTGAGGCTGAGGCTGAAGAAGGGCTTGACCTTTCAGGTTCAGAGAGGTTTGCGTTTTCTGTGGATGATGCTCTCACTGTCAGCAATGCTGGTTCGGCTGGTTCTGCTGAGAGTAGGAGGTGGCTTTTGTTGTCGAAATTGAAGCAGGGATTATCTAAGCATAAGGATGGCCAGGGTCCTGGTAAGAAGCTGTTTCCGTGGAGGTTTGGTTTGGAGGATAAAGAGAAGGTTGAGGTTGAGAATAGAGTGGAGGAGAGGTCTGCTCCGCCTGGTAGGAGAAGGAAGGAGGGTCCGGTCGATCATGAATTGGTGTTGAGGGCATTGTCAAGGGCTTTACAGGTGACGGAGCTTGCTTATTTGGATATGACTGATAAGGTCATTGATACAAATCCAGAGCTTGCTTTGATGGGGTCGTGTTTGTTGGTTGTGCTAATGAGGGATGAAGATGTGTATGTGATGAATGTGGGGGATAGTAGGGCGATTGTTGCACATTATGAGCCGGAGGAAGTTGGTACAAGCGTGAAATCAAGGGGGCAGAAGGACAATGGGTTGAGTGCTGAGGACATAACTGAGGCATCCTCAGCTGTGGGCGATGAGGCTCCTACTCAACCAATGAGATTGGCTGCATTGCAGCTGTCCACTGATCACAGCACAAGCATTGAAGAAGAAGTGATAAGAATCAAGAATGAACACCCAGATGACAAAAACTGTATTCTCAATGATAGAGTGAAAGGTCGTCTTAAGGTTACCAGAGCGTTTGGGGCAGGCTTTCTGAAACAGGTCCTATGGATGTACTTGTTAATTGTCCTAATATATATCACTTTTTGCGTAGTTTGCTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0005488 GO:0005543 GO:0005575 GO:0005623 GO:0005886 GO:0006355 GO:0008150 GO:0008289 GO:0009653 GO:0009826 GO:0009888 GO:0009889 GO:0009933 GO:0009987 GO:0010073 GO:0010074 GO:0010468 GO:0010556 GO:0016020 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0019827 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043167 GO:0043168 GO:0044464 GO:0048507 GO:0048532 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0060560 GO:0065007 GO:0071840 GO:0071944 GO:0080090 GO:0098727 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C

Protein Analysis

677

Amino Acids

73.71

Weight (kDa)

5.29

Isoelectric Point (pI)

45.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	608 - 657	7.7e-07	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011099)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35350
fragaria_vesca	FvH4_5g21630 FvH4_5g21630
malus_domestica	MD06G1031300.v1.1 MD16G1277400.v1.1
prunus_persica	Prupe.5G040900_v2.0.a1
pyrus_communis	pycom06g02610 pycom16g24670
rosa_chinensis	RchiOBHm_Chr7g0209091
rosa_laevigata	RLG00000003168
rosa_multiflora	Rmu_co8472153.1_g000001 Rmu_sc0000821.1_g000009 Rmu_sc0012788.1_g000001
rosa_roxburghii	Rroxscaffold_3G00249760
rosa_rugosa	Rorug07G0109000
rosa_samantha	Rh7AG243400 Rh7BG238500 Rh7CG258600 Rh7DG250400
rosa_wichuraiana	Rw7G020630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1115
AasI	GACNNNNNNGTC	1 cut(s)	557
AatII	GACGTC	1 cut(s)	72
Acc36I	ACCTGC	1 cut(s)	1115
AccB1I	GGYRCC	5 cut(s)	266, 436, 571, 676, 1170
AccBSI	CCGCTC	3 cut(s)	140, 526, 577
AccII	CGCG	4 cut(s)	222, 442, 474, 507
AccIII	TCCGGA	1 cut(s)	627
AclWI	GGATC	3 cut(s)	174, 619, 632
AcoI	YGGCCR	2 cut(s)	700, 1360
AcsI	RAATTY	1 cut(s)	1105
AcuI	CTGAAG	4 cut(s)	19, 908, 1029, 1227
AcyI	GRCGYC	4 cut(s)	69, 267, 572, 1171
AfaI	GTAC	5 cut(s)	403, 804, 1024, 1705, 1985
AflII	CTTAAG	1 cut(s)	1931
AflIII	ACRYGT	1 cut(s)	805
AgsI	TTSAA	4 cut(s)	253, 1154, 1333, 1853
AhlI	ACTAGT	1 cut(s)	1019
AjnI	CCWGG	4 cut(s)	668, 1362, 1369, 1459
AjuI	GAANNNNNNNTTGG	2 cut(s)	1027, 1059
AluBI	AGCT	9 cut(s)	288, 313, 922, 1029, 1381, 1540, 1588, 1775, 1825
AluI	AGCT	9 cut(s)	288, 313, 922, 1029, 1381, 1540, 1588, 1775, 1825
Alw21I	GWGCWC	2 cut(s)	687, 738
Alw26I	GTCTC	4 cut(s)	19, 236, 734, 752
AlwI	GGATC	3 cut(s)	174, 619, 632
Ama87I	CYCGRG	2 cut(s)	239, 1117
Aor13HI	TCCGGA	1 cut(s)	627
ApeKI	GCWGC	3 cut(s)	378, 1814, 1822
ApoI	RAATTY	1 cut(s)	1105
AspA2I	CCTAGG	1 cut(s)	106
AspLEI	GCGC	8 cut(s)	224, 269, 474, 507, 514, 574, 877, 1173
AsuC2I	CCSGG	8 cut(s)	226, 231, 247, 298, 543, 622, 790, 800
AsuHPI	GGTGA	5 cut(s)	38, 1023, 1064, 1139, 1543
AsuII	TTCGAA	1 cut(s)	1103
AvaI	CYCGRG	2 cut(s)	239, 1117
AvaII	GGWCC	9 cut(s)	132, 494, 521, 545, 796, 1095, 1367, 1481, 1972
AvrII	CCTAGG	1 cut(s)	106
BaeGI	GKGCMC	1 cut(s)	421
BaeI	ACNNNNGTAYC	2 cut(s)	1695, 1728
BalI	TGGCCA	1 cut(s)	1362
BamHI	GGATCC	1 cut(s)	624
BanI	GGYRCC	5 cut(s)	266, 436, 571, 676, 1170
BanII	GRGCYC	3 cut(s)	93, 456, 778
BarI	GAAGNNNNNNTAC	2 cut(s)	623, 655
BauI	CACGAG	2 cut(s)	212, 737
BbsI	GAAGAC	1 cut(s)	67
Bbv12I	GWGCWC	2 cut(s)	687, 738
BbvCI	CCTCAGC	3 cut(s)	1200, 1749, 1771
BbvI	GCAGC	3 cut(s)	390, 1801, 1834
BccI	CCATC	6 cut(s)	266, 848, 883, 1061, 1352, 1591
BceAI	ACGGC	4 cut(s)	568, 680, 759, 880
BciT130I	CCWGG	4 cut(s)	670, 1364, 1371, 1461
BclI	TGATCA	1 cut(s)	1834
BcnI	CCSGG	8 cut(s)	226, 231, 247, 298, 543, 622, 790, 800
BcoDI	GTCTC	4 cut(s)	19, 236, 734, 752
BcuI	ACTAGT	1 cut(s)	1019
BfaI	CTAG	3 cut(s)	107, 1020, 1026
BfoI	RGCGCY	4 cut(s)	270, 515, 575, 1174
BfrI	CTTAAG	1 cut(s)	1931
BfuAI	ACCTGC	1 cut(s)	1115
BlnI	CCTAGG	1 cut(s)	106
BmcAI	AGTACT	1 cut(s)	1024
Bme18I	GGWCC	9 cut(s)	132, 494, 521, 545, 796, 1095, 1367, 1481, 1972
BmeT110I	CYCGRG	2 cut(s)	239, 1117
BmsI	GCATC	6 cut(s)	322, 390, 862, 1072, 1249, 1775
BoxI	GACNNNNGTC	1 cut(s)	764
BpiI	GAAGAC	1 cut(s)	67
BplI	GAGNNNNNCTC	4 cut(s)	1438, 1470, 1754, 1786
BpmI	CTGGAG	2 cut(s)	293, 413
Bpu10I	CCTNAGC	3 cut(s)	1200, 1749, 1771
Bpu14I	TTCGAA	1 cut(s)	1103
BpuMI	CCSGG	8 cut(s)	226, 231, 247, 298, 543, 622, 790, 800
BsaBI	GATNNNNATC	2 cut(s)	1578, 1868
BsaHI	GRCGYC	4 cut(s)	69, 267, 572, 1171
BsaI	GGTCTC	2 cut(s)	236, 752
BsaWI	WCCGGW	7 cut(s)	195, 491, 518, 547, 627, 1156, 1483
BsaXI	ACNNNNNCTCC	6 cut(s)	815, 845, 1384, 1414, 1433, 1463
Bse118I	RCCGGY	4 cut(s)	433, 679, 718, 1173
Bse1I	ACTGG	1 cut(s)	769
Bse3DI	GCAATG	2 cut(s)	1282, 1817
Bse8I	GATNNNNATC	2 cut(s)	1578, 1868
BseAI	TCCGGA	1 cut(s)	627
BseBI	CCWGG	4 cut(s)	670, 1364, 1371, 1461
BseGI	GGATG	6 cut(s)	1087, 1261, 1363, 1636, 1766, 1986
BseJI	GATNNNNATC	2 cut(s)	1578, 1868
BseMI	GCAATG	2 cut(s)	1282, 1817
BseMII	CTCAG	7 cut(s)	173, 330, 1191, 1290, 1740, 1752, 1785
BseNI	ACTGG	1 cut(s)	769
BseRI	GAGGAG	5 cut(s)	16, 746, 836, 1180, 1457
BseSI	GKGCMC	1 cut(s)	421
BseX3I	CGGCCG	1 cut(s)	700
BseXI	GCAGC	3 cut(s)	390, 1801, 1834
Bsh1236I	CGCG	4 cut(s)	222, 442, 474, 507
Bsh1285I	CGRYCG	2 cut(s)	703, 1488
BshNI	GGYRCC	5 cut(s)	266, 436, 571, 676, 1170
BsiEI	CGRYCG	2 cut(s)	703, 1488
BsiHKAI	GWGCWC	2 cut(s)	687, 738
BsiHKCI	CYCGRG	2 cut(s)	239, 1117
BsmAI	GTCTC	4 cut(s)	19, 236, 734, 752
BsmBI	CGTCTC	1 cut(s)	734
Bso31I	GGTCTC	2 cut(s)	236, 752
BsoBI	CYCGRG	2 cut(s)	239, 1117
Bsp119I	TTCGAA	1 cut(s)	1103
Bsp1286I	GDGCHC	6 cut(s)	93, 421, 456, 687, 738, 778
Bsp13I	TCCGGA	1 cut(s)	627
Bsp143I	GATC	6 cut(s)	38, 166, 624, 1129, 1489, 1834
BspCNI	CTCAG	7 cut(s)	172, 331, 1192, 1291, 1741, 1753, 1784
BspEI	TCCGGA	1 cut(s)	627
BspFNI	CGCG	4 cut(s)	222, 442, 474, 507
BspHI	TCATGA	1 cut(s)	1492
BspMI	ACCTGC	1 cut(s)	1115
BspPI	GGATC	3 cut(s)	174, 619, 632
BspT104I	TTCGAA	1 cut(s)	1103
BspT107I	GGYRCC	5 cut(s)	266, 436, 571, 676, 1170
BspTI	CTTAAG	1 cut(s)	1931
BspTNI	GGTCTC	2 cut(s)	236, 752
BsrBI	CCGCTC	3 cut(s)	140, 526, 577
BsrDI	GCAATG	2 cut(s)	1282, 1817
BsrFI	RCCGGY	4 cut(s)	433, 679, 718, 1173
BsrI	ACTGG	1 cut(s)	769
BssAI	RCCGGY	4 cut(s)	433, 679, 718, 1173
BssMI	GATC	6 cut(s)	38, 166, 624, 1129, 1489, 1834
BssNI	GRCGYC	4 cut(s)	69, 267, 572, 1171
BssSI	CACGAG	2 cut(s)	212, 737
BssT1I	CCWWGG	2 cut(s)	93, 106
Bst2BI	CACGAG	2 cut(s)	212, 737
Bst2UI	CCWGG	4 cut(s)	670, 1364, 1371, 1461
Bst4CI	ACNGT	5 cut(s)	304, 353, 905, 1270, 1901
Bst6I	CTCTTC	1 cut(s)	593
BstACI	GRCGYC	4 cut(s)	69, 267, 572, 1171
BstAFI	CTTAAG	1 cut(s)	1931
BstBI	TTCGAA	1 cut(s)	1103
BstC8I	GCNNGC	7 cut(s)	142, 220, 452, 1175, 1542, 1590, 1957
BstDEI	CTNAG	8 cut(s)	159, 339, 1200, 1299, 1347, 1749, 1761, 1771
BstDSI	CCRYGG	2 cut(s)	440, 1388
BstEII	GGTNACC	1 cut(s)	1936
BstF5I	GGATG	6 cut(s)	1087, 1261, 1363, 1636, 1766, 1986
BstFNI	CGCG	4 cut(s)	222, 442, 474, 507
BstH2I	RGCGCY	4 cut(s)	270, 515, 575, 1174
BstHHI	GCGC	8 cut(s)	224, 269, 474, 507, 514, 574, 877, 1173
BstKTI	GATC	6 cut(s)	41, 169, 627, 1132, 1492, 1837
BstMAI	GTCTC	4 cut(s)	19, 236, 734, 752
BstMBI	GATC	6 cut(s)	38, 166, 624, 1129, 1489, 1834
BstMCI	CGRYCG	2 cut(s)	703, 1488
BstNI	CCWGG	4 cut(s)	670, 1364, 1371, 1461
BstNSI	RCATGY	1 cut(s)	809
BstPAI	GACNNNNGTC	1 cut(s)	764
BstPI	GGTNACC	1 cut(s)	1936
BstSLI	GKGCMC	1 cut(s)	421
BstUI	CGCG	4 cut(s)	222, 442, 474, 507
BstV1I	GCAGC	3 cut(s)	390, 1801, 1834
BstV2I	GAAGAC	1 cut(s)	67
BstX2I	RGATCY	1 cut(s)	624
BstXI	CCANNNNNNTGG	1 cut(s)	1370
BstYI	RGATCY	1 cut(s)	624
BstZI	CGGCCG	1 cut(s)	700
BtgI	CCRYGG	2 cut(s)	440, 1388
BtgZI	GCGATG	2 cut(s)	570, 1797
BtsCI	GGATG	6 cut(s)	1087, 1261, 1363, 1636, 1766, 1986
BtsIMutI	CAGTG	5 cut(s)	283, 336, 776, 1266, 1830
BveI	ACCTGC	1 cut(s)	1115
Cac8I	GCNNGC	7 cut(s)	142, 220, 452, 1175, 1542, 1590, 1957
CciI	TCATGA	1 cut(s)	1492
CfoI	GCGC	8 cut(s)	224, 269, 474, 507, 514, 574, 877, 1173
Cfr10I	RCCGGY	4 cut(s)	433, 679, 718, 1173
Cfr42I	CCGCGG	1 cut(s)	443
CpoI	CGGWCCG	3 cut(s)	494, 521, 545
Csp6I	GTAC	5 cut(s)	402, 803, 1023, 1704, 1984
CspI	CGGWCCG	3 cut(s)	494, 521, 545
CviAII	CATG	3 cut(s)	806, 977, 1493
CviQI	GTAC	5 cut(s)	402, 803, 1023, 1704, 1984
DdeI	CTNAG	8 cut(s)	159, 339, 1200, 1299, 1347, 1749, 1761, 1771
DinI	GGCGCC	3 cut(s)	268, 573, 1172
DpnI	GATC	6 cut(s)	40, 168, 626, 1131, 1491, 1836
DpnII	GATC	6 cut(s)	38, 166, 624, 1129, 1489, 1834
DrdI	GACNNNNNNGTC	1 cut(s)	557
DseDI	GACNNNNNNGTC	1 cut(s)	557
EaeI	YGGCCR	2 cut(s)	700, 1360
EagI	CGGCCG	1 cut(s)	700
Eam1104I	CTCTTC	1 cut(s)	593
EarI	CTCTTC	1 cut(s)	593
EciI	GGCGGA	4 cut(s)	70, 381, 619, 1446
EclXI	CGGCCG	1 cut(s)	700
Eco130I	CCWWGG	2 cut(s)	93, 106
Eco24I	GRGCYC	3 cut(s)	93, 456, 778
Eco31I	GGTCTC	2 cut(s)	236, 752
Eco47I	GGWCC	9 cut(s)	132, 494, 521, 545, 796, 1095, 1367, 1481, 1972
Eco52I	CGGCCG	1 cut(s)	700
Eco57I	CTGAAG	4 cut(s)	19, 908, 1029, 1227
Eco88I	CYCGRG	2 cut(s)	239, 1117
Eco91I	GGTNACC	1 cut(s)	1936
EcoO109I	RGGNCCY	3 cut(s)	132, 1367, 1972
EcoO65I	GGTNACC	1 cut(s)	1936
EcoRI	GAATTC	1 cut(s)	1105
EcoRII	CCWGG	4 cut(s)	668, 1362, 1369, 1459
EcoT14I	CCWWGG	2 cut(s)	93, 106
EcoT38I	GRGCYC	3 cut(s)	93, 456, 778
EgeI	GGCGCC	3 cut(s)	268, 573, 1172
EheI	GGCGCC	3 cut(s)	268, 573, 1172
ErhI	CCWWGG	2 cut(s)	93, 106
Esp3I	CGTCTC	1 cut(s)	734
FaeI	CATG	3 cut(s)	809, 980, 1496
FalI	AAGNNNNNCTT	4 cut(s)	1200, 1232, 1914, 1946
FatI	CATG	3 cut(s)	805, 976, 1492
FauI	CCCGC	4 cut(s)	227, 435, 500, 546
FbaI	TGATCA	1 cut(s)	1834
FokI	GGATG	6 cut(s)	1094, 1268, 1370, 1643, 1753, 1993
FriOI	GRGCYC	3 cut(s)	93, 456, 778
FspBI	CTAG	3 cut(s)	107, 1020, 1026
GlaI	GCGC	8 cut(s)	223, 268, 473, 506, 513, 573, 876, 1172
GsuI	CTGGAG	2 cut(s)	293, 413
HaeII	RGCGCY	4 cut(s)	270, 515, 575, 1174
HhaI	GCGC	8 cut(s)	224, 269, 474, 507, 514, 574, 877, 1173
Hin1I	GRCGYC	4 cut(s)	69, 267, 572, 1171
Hin1II	CATG	3 cut(s)	809, 980, 1496
Hin6I	GCGC	8 cut(s)	222, 267, 472, 505, 512, 572, 875, 1171
HinP1I	GCGC	8 cut(s)	222, 267, 472, 505, 512, 572, 875, 1171
HinfI	GANTC	4 cut(s)	12, 530, 895, 1869
HphI	GGTGA	5 cut(s)	38, 1023, 1064, 1139, 1543
Hpy166II	GTNNAC	3 cut(s)	349, 562, 1830
Hpy188III	TCNNGA	7 cut(s)	397, 628, 652, 886, 1493, 1583, 1873
Hpy8I	GTNNAC	3 cut(s)	349, 562, 1830
Hpy99I	CGWCG	6 cut(s)	71, 74, 332, 409, 412, 701
HpyCH4III	ACNGT	5 cut(s)	304, 353, 905, 1270, 1901
HpyCH4IV	ACGT	2 cut(s)	69, 404
HpyCH4V	TGCA	4 cut(s)	980, 1679, 1817, 1822
HpyF3I	CTNAG	8 cut(s)	159, 339, 1200, 1299, 1347, 1749, 1761, 1771
HpySE526I	ACGT	2 cut(s)	69, 404
Hsp92I	GRCGYC	4 cut(s)	69, 267, 572, 1171
Hsp92II	CATG	3 cut(s)	809, 980, 1496
HspAI	GCGC	8 cut(s)	222, 267, 472, 505, 512, 572, 875, 1171
KasI	GGCGCC	3 cut(s)	266, 571, 1170
Kpn2I	TCCGGA	1 cut(s)	627
KroI	GCCGGC	1 cut(s)	1173
KroNI	GCCGGC	1 cut(s)	1175
Ksp22I	TGATCA	1 cut(s)	1834
KspI	CCGCGG	1 cut(s)	443
Kzo9I	GATC	6 cut(s)	38, 166, 624, 1129, 1489, 1834
LmnI	GCTCC	7 cut(s)	293, 469, 733, 823, 1459, 1537, 1795
Lsp1109I	GCAGC	3 cut(s)	390, 1801, 1834
LweI	GCATC	6 cut(s)	322, 390, 862, 1072, 1249, 1775
MaeI	CTAG	3 cut(s)	107, 1020, 1026
MaeII	ACGT	2 cut(s)	69, 404
MaeIII	GTNAC	4 cut(s)	1144, 1185, 1531, 1936
MalI	GATC	6 cut(s)	40, 168, 626, 1131, 1491, 1836
MbiI	CCGCTC	3 cut(s)	140, 526, 577
MboI	GATC	6 cut(s)	38, 166, 624, 1129, 1489, 1834
MboII	GAAGA	8 cut(s)	67, 610, 619, 667, 1220, 1646, 1865, 1868
MflI	RGATCY	1 cut(s)	624
MhlI	GDGCHC	6 cut(s)	93, 421, 456, 687, 738, 778
MlsI	TGGCCA	1 cut(s)	1362
MluCI	AATT	7 cut(s)	691, 1062, 1099, 1105, 1328, 1496, 1992
MluNI	TGGCCA	1 cut(s)	1362
Mly113I	GGCGCC	3 cut(s)	267, 572, 1171
MlyI	GAGTC	2 cut(s)	21, 524
MmeI	TCCRAC	2 cut(s)	144, 210
Mox20I	TGGCCA	1 cut(s)	1362
MroI	TCCGGA	1 cut(s)	627
MroNI	GCCGGC	1 cut(s)	1173
MscI	TGGCCA	1 cut(s)	1362
MseI	TTAA	5 cut(s)	861, 1008, 1932, 1991, 2032
MslI	CAYNNNNRTG	2 cut(s)	1356, 1887
Msp20I	TGGCCA	1 cut(s)	1362
MspA1I	CMGCKG	3 cut(s)	442, 1775, 1825
MspCI	CTTAAG	1 cut(s)	1931
MvaI	CCWGG	4 cut(s)	670, 1364, 1371, 1461
MvnI	CGCG	4 cut(s)	222, 442, 474, 507
NaeI	GCCGGC	1 cut(s)	1175
NarI	GGCGCC	3 cut(s)	267, 572, 1171
NciI	CCSGG	8 cut(s)	226, 231, 247, 298, 543, 622, 790, 800
NdeII	GATC	6 cut(s)	38, 166, 624, 1129, 1489, 1834
NgoMIV	GCCGGC	1 cut(s)	1173
NlaIII	CATG	3 cut(s)	809, 980, 1496
NmeAIII	GCCGAG	3 cut(s)	548, 558, 759
NmuCI	GTSAC	2 cut(s)	1144, 1531
NspI	RCATGY	1 cut(s)	809
NspV	TTCGAA	1 cut(s)	1103
PagI	TCATGA	1 cut(s)	1492
PaqCI	CACCTGC	1 cut(s)	1115
PasI	CCCWGGG	1 cut(s)	669
PciI	ACATGT	1 cut(s)	805
PdiI	GCCGGC	1 cut(s)	1175
PfeI	GAWTC	2 cut(s)	895, 1869
PflFI	GACNNNGTC	1 cut(s)	71
PfoI	TCCNGGA	1 cut(s)	620
PleI	GAGTC	2 cut(s)	20, 524
PluTI	GGCGCC	3 cut(s)	270, 575, 1174
PpsI	GAGTC	2 cut(s)	20, 524
PpuMI	RGGWCCY	3 cut(s)	132, 1367, 1972
PscI	ACATGT	1 cut(s)	805
PshAI	GACNNNNGTC	1 cut(s)	764
Psp5II	RGGWCCY	3 cut(s)	132, 1367, 1972
Psp6I	CCWGG	4 cut(s)	668, 1362, 1369, 1459
PspEI	GGTNACC	1 cut(s)	1936
PspGI	CCWGG	4 cut(s)	668, 1362, 1369, 1459
PspPPI	RGGWCCY	3 cut(s)	132, 1367, 1972
PsuI	RGATCY	1 cut(s)	624
PsyI	GACNNNGTC	1 cut(s)	71
PvuII	CAGCTG	2 cut(s)	1775, 1825
RsaI	GTAC	5 cut(s)	403, 804, 1024, 1705, 1985
RsaNI	GTAC	5 cut(s)	402, 803, 1023, 1704, 1984
RseI	CAYNNNNRTG	2 cut(s)	1356, 1887
Rsr2I	CGGWCCG	3 cut(s)	494, 521, 545
RsrII	CGGWCCG	3 cut(s)	494, 521, 545
SacII	CCGCGG	1 cut(s)	443
SaqAI	TTAA	5 cut(s)	861, 1008, 1932, 1991, 2032
Sau3AI	GATC	6 cut(s)	38, 166, 624, 1129, 1489, 1834
ScaI	AGTACT	1 cut(s)	1024
SchI	GAGTC	2 cut(s)	21, 524
SduI	GDGCHC	6 cut(s)	93, 421, 456, 687, 738, 778
SfaNI	GCATC	6 cut(s)	322, 390, 862, 1072, 1249, 1775
SfoI	GGCGCC	3 cut(s)	268, 573, 1172
Sfr303I	CCGCGG	1 cut(s)	443
SfuI	TTCGAA	1 cut(s)	1103
SgrAI	CRCCGGYG	1 cut(s)	718
SgrBI	CCGCGG	1 cut(s)	443
SinI	GGWCC	9 cut(s)	132, 494, 521, 545, 796, 1095, 1367, 1481, 1972
SmiMI	CAYNNNNRTG	2 cut(s)	1356, 1887
SmlI	CTYRAG	1 cut(s)	1931
SmoI	CTYRAG	1 cut(s)	1931
SpeI	ACTAGT	1 cut(s)	1019
Sse9I	AATT	7 cut(s)	691, 1062, 1099, 1105, 1328, 1496, 1992
SspDI	GGCGCC	3 cut(s)	266, 571, 1170
SspI	AATATT	1 cut(s)	322
SspMI	CTAG	3 cut(s)	107, 1020, 1026
StyI	CCWWGG	2 cut(s)	93, 106
TaaI	ACNGT	5 cut(s)	304, 353, 905, 1270, 1901
TaiI	ACGT	2 cut(s)	72, 407
TaqI	TCGA	7 cut(s)	396, 528, 533, 651, 1103, 1325, 1488
TasI	AATT	7 cut(s)	691, 1062, 1099, 1105, 1328, 1496, 1992
TatI	WGTACW	2 cut(s)	1022, 1983
TauI	GCSGC	7 cut(s)	208, 211, 366, 442, 477, 480, 577
TfiI	GAWTC	2 cut(s)	895, 1869
Tru1I	TTAA	5 cut(s)	861, 1008, 1932, 1991, 2032
Tru9I	TTAA	5 cut(s)	861, 1008, 1932, 1991, 2032
TscAI	CASTG	5 cut(s)	283, 343, 776, 1273, 1837
TseFI	GTSAC	2 cut(s)	1144, 1531
TseI	GCWGC	3 cut(s)	378, 1814, 1822
Tsp45I	GTSAC	2 cut(s)	1144, 1531
TspDTI	ATGAA	4 cut(s)	1509, 1647, 1664, 1893
TspGWI	ACGGA	4 cut(s)	262, 1181, 1377, 1550
TspRI	CASTG	5 cut(s)	283, 343, 776, 1273, 1837
Tth111I	GACNNNGTC	1 cut(s)	71
Vha464I	CTTAAG	1 cut(s)	1931
VpaK11BI	GGWCC	9 cut(s)	132, 494, 521, 545, 796, 1095, 1367, 1481, 1972
XapI	RAATTY	1 cut(s)	1105
XceI	RCATGY	1 cut(s)	809
XmaJI	CCTAGG	1 cut(s)	106
XspI	CTAG	3 cut(s)	107, 1020, 1026
ZraI	GACGTC	1 cut(s)	70
ZrmI	AGTACT	1 cut(s)	1024

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.