AT1G01450

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013904
Physical Location & Seq
Reverse (-)
164105 .. 165517
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G01450.1

Sequence Viewer

Length: 1413 bp
ATGGCTGATTTCCTTTTGAAGCATTTGGGAGATGGAAATGAGAGCCCCAAGCTATTTCCTTCTTCTCTTCTGGATAACACAAAAGATTACCAAGTGAAAAAAAGATTAGGGAATGGAAGTCAGTACAAGGAGATTACCTGGTTAGGCGAGAGCTTTGCGCTTAGGCATTTCTTTGGGGATATTGATGCTTTGCTTCCTCAGATTACTCCATTGCTATCTCTTTCACACCCAAACATTGTATATTACCTCTGCGGATTCACGGATGAGGAGAAGAAAGAGTGTTTCTTGGTCATGGAACTGATGAGGAAAACCCTTGGAATGCACATCAAAGAGGTATGTGGTCCTAGGAAGAAGAACACTCTCTCCCTCCCGGTCGCGGTTGATCTGATGCTTCAGATAGCTTTGGGTATGGAATATCTTCACTCGAAGAGAATATACCACGGAGAGTTGAATCCATCAAACATTCTTGTCAAACCAAGAAGTAACCAATCCGGAGATGGGTATCTGCTTGGGAAGATTTTTGGGTTCGGGTTGAATTCTGTCAAGGGATTCTCTAGTAAAAGTGCTTCTTTGACGAGTCAGAATGAGAATTTTCCATTCATATGGTATTCTCCAGAAGTTCTAGAGGAGCAAGAACAGAGTGGAACTGCAGGAAGCCTTAAGTATAGTGATAAATCTGACGTGTATAGCTTTGGGATGGTTAGCTTTGAGCTTCTGACAGGTAAAGTACCATTTGAAGATAGCCACCTGCAAGGAGATAAGATGAGTAGAAACATTAGGGCCGGGGAGAGACCGCTTTTTCCATTTAATTCACCAAAGTTCATAACAAACCTGACAAAACGATGCTGGCATGCTGATCCCAATCAGCGTCCAACTTTCTCATCGATAAGCAGAATTCTCCGGTATATCAAACGGTTTCTAGCTTTGAACCCGGAATGTTACAGTAGTAGCCAACAAGACCCGTCCATTGCGCCTACTGTGGATTACTGCGAGATCGAGACAAAGCTATTGCAGAAGCTTTCTTGGGAATCTACAGAGCTAACCAAAGTTTCACAGGTTCCTTTTCAAATGTTTGCATATAGGGTTGTGGAACGGGCAAAAACGTGTGAAAAGGATAATCTCCGGGAACCATCGGAATCAGGAAGTGAATGGGCTTCGTGTAGCGAGGATGAGGGTGGGGCAGGATCAGATGAGCAGTTGTCATATGCCAAGGAAAGAAGATTGTCATGCTCCTCAAATGATGTTGGTATGAGCAAGAAGCAGGTTTCAAATCTTCTGAAAAGAGCTTCTAGCCTGAAACCAATTCAAAAACCAGGTGAGATTATCATCAGCCAATATATATATATATATATAGGCTCCCTAACAAATATGAACTTGGTTACTTGTACTAATTTTTTTGTTTTGTGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

470

Amino Acids

53.12

Weight (kDa)

7.9

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 64 - 299 2e-37 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 68 - 296 5.6e-28 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013904)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01450
fragaria_vesca FvH4_7g15980
malus_domestica MD01G1076300.v1.1 MD07G1145200.v1.1
prunus_persica Prupe.2G183700_v2.0.a1 Prupe.2G183700_v2.0.a1
pyrus_communis pycom07g14470
rosa_chinensis RchiOBHm_Chr1g0358861
rosa_laevigata RLG00000027921
rosa_multiflora Rmu_sc0008757.1_g000014
rosa_roxburghii Rroxscaffold_4G00297570
rosa_rugosa Rorug01G0265700
rosa_samantha Rh1AG279600 Rh1BG246400 Rh1CG263800 Rh1DG274800
rosa_wichuraiana Rw1G024860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 756
Acc36I ACCTGC 2 cut(s) 756, 1252
AccII CGCG 1 cut(s) 377
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 3 cut(s) 252, 377, 794
AclWI GGATC 2 cut(s) 851, 1192
AcsI RAATTY 3 cut(s) 535, 589, 894
AcuI CTGAAG 1 cut(s) 377
AfaI GTAC 3 cut(s) 125, 729, 1387
AfiI CCNNNNNNNGG 2 cut(s) 376, 498
AflII CTTAAG 1 cut(s) 659
AflIII ACRYGT 2 cut(s) 681, 1103
AgsI TTSAA 8 cut(s) 19, 451, 535, 737, 928, 1067, 1269, 1307
AjiI CACGTC 1 cut(s) 682
AjnI CCWGG 2 cut(s) 137, 1312
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
Alw26I GTCTC 2 cut(s) 784, 992
AlwI GGATC 2 cut(s) 851, 1192
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 1 cut(s) 780
ApoI RAATTY 3 cut(s) 535, 589, 894
Asp700I GAANNNNTTC 1 cut(s) 417
AspA2I CCTAGG 1 cut(s) 344
AspLEI GCGC 2 cut(s) 160, 973
AspS9I GGNCC 2 cut(s) 341, 780
AsuC2I CCSGG 4 cut(s) 371, 784, 932, 1124
AsuHPI GGTGA 2 cut(s) 804, 1328
AvaII GGWCC 1 cut(s) 341
AvrII CCTAGG 1 cut(s) 344
BanII GRGCYC 1 cut(s) 47
BarI GAAGNNNNNNTAC 4 cut(s) 419, 451, 550, 582
BccI CCATC 5 cut(s) 26, 463, 491, 691, 1138
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 2 cut(s) 139, 1314
BcnI CCSGG 4 cut(s) 371, 784, 932, 1124
BcoDI GTCTC 2 cut(s) 784, 992
BfaI CTAG 6 cut(s) 345, 555, 623, 920, 1290, 1411
BfmI CTRYAG 2 cut(s) 648, 1032
BfrI CTTAAG 1 cut(s) 659
BfuAI ACCTGC 2 cut(s) 756, 1252
BlnI CCTAGG 1 cut(s) 344
Bme1390I CCNGG 6 cut(s) 139, 371, 784, 932, 1124, 1314
Bme18I GGWCC 1 cut(s) 341
BmgBI CACGTC 1 cut(s) 682
BmgT120I GGNCC 2 cut(s) 341, 780
BmiI GGNNCC 3 cut(s) 1059, 1128, 1357
BmrFI CCNGG 6 cut(s) 139, 371, 784, 932, 1124, 1314
BmsI GCATC 3 cut(s) 175, 378, 833
BpmI CTGGAG 1 cut(s) 597
Bpu10I CCTNAGC 1 cut(s) 161
BpuMI CCSGG 4 cut(s) 371, 784, 932, 1124
Bsa29I ATCGAT 1 cut(s) 884
BsaBI GATNNNNATC 3 cut(s) 501, 861, 1325
BsaI GGTCTC 1 cut(s) 784
BsaJI CCNNGG 5 cut(s) 313, 344, 439, 783, 1209
BsaWI WCCGGW 2 cut(s) 491, 900
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 498
Bse3DI GCAATG 2 cut(s) 209, 966
Bse8I GATNNNNATC 3 cut(s) 501, 861, 1325
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 2 cut(s) 139, 1314
BseCI ATCGAT 1 cut(s) 884
BseDI CCNNGG 5 cut(s) 313, 344, 439, 783, 1209
BseGI GGATG 3 cut(s) 268, 702, 1174
BseJI GATNNNNATC 3 cut(s) 501, 861, 1325
BseLI CCNNNNNNNGG 2 cut(s) 376, 498
BseMI GCAATG 2 cut(s) 209, 966
BseMII CTCAG 1 cut(s) 212
BseRI GAGGAG 3 cut(s) 281, 641, 1222
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 1 cut(s) 782
BshVI ATCGAT 1 cut(s) 884
BsiEI CGRYCG 1 cut(s) 375
BsiSI CCGG 6 cut(s) 371, 492, 783, 901, 932, 1123
BslI CCNNNNNNNGG 2 cut(s) 376, 498
BsmAI GTCTC 2 cut(s) 784, 992
BsmI GAATGC 1 cut(s) 324
BsnI GGCC 1 cut(s) 782
Bso31I GGTCTC 1 cut(s) 784
Bsp1286I GDGCHC 1 cut(s) 47
Bsp13I TCCGGA 1 cut(s) 491
Bsp143I GATC 4 cut(s) 382, 856, 993, 1184
BspACI CCGC 3 cut(s) 252, 377, 794
BspANI GGCC 1 cut(s) 782
BspCNI CTCAG 1 cut(s) 211
BspDI ATCGAT 1 cut(s) 884
BspEI TCCGGA 1 cut(s) 491
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 3 cut(s) 1059, 1128, 1357
BspMAI CTGCAG 1 cut(s) 652
BspMI ACCTGC 2 cut(s) 756, 1252
BspPI GGATC 2 cut(s) 851, 1192
BspTI CTTAAG 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 784
BsrDI GCAATG 2 cut(s) 209, 966
BssECI CCNNGG 5 cut(s) 313, 344, 439, 783, 1209
BssMI GATC 4 cut(s) 382, 856, 993, 1184
BssT1I CCWWGG 3 cut(s) 313, 344, 1209
Bst2UI CCWGG 2 cut(s) 139, 1314
Bst4CI ACNGT 3 cut(s) 915, 944, 979
Bst6I CTCTTC 2 cut(s) 72, 422
BstAFI CTTAAG 1 cut(s) 659
BstC8I GCNNGC 2 cut(s) 848, 852
BstDEI CTNAG 2 cut(s) 161, 198
BstDSI CCRYGG 1 cut(s) 439
BstF5I GGATG 3 cut(s) 268, 702, 1174
BstFNI CGCG 1 cut(s) 377
BstHHI GCGC 2 cut(s) 160, 973
BstKTI GATC 4 cut(s) 385, 859, 996, 1187
BstMAI GTCTC 2 cut(s) 784, 992
BstMBI GATC 4 cut(s) 382, 856, 993, 1184
BstMCI CGRYCG 1 cut(s) 375
BstNI CCWGG 2 cut(s) 139, 1314
BstNSI RCATGY 1 cut(s) 854
BstSCI CCNGG 6 cut(s) 137, 369, 782, 930, 1122, 1312
BstSFI CTRYAG 2 cut(s) 648, 1032
BstUI CGCG 1 cut(s) 377
BstXI CCANNNNNNTGG 1 cut(s) 603
Bsu15I ATCGAT 1 cut(s) 884
BsuRI GGCC 1 cut(s) 782
BsuTUI ATCGAT 1 cut(s) 884
BtgI CCRYGG 1 cut(s) 439
BtrI CACGTC 1 cut(s) 682
BtsCI GGATG 3 cut(s) 268, 702, 1174
BveI ACCTGC 2 cut(s) 756, 1252
Cac8I GCNNGC 2 cut(s) 848, 852
CfoI GCGC 2 cut(s) 160, 973
Cfr13I GGNCC 2 cut(s) 341, 780
ClaI ATCGAT 1 cut(s) 884
CseI GACGC 1 cut(s) 857
CsiI ACCWGGT 2 cut(s) 137, 1312
Csp6I GTAC 3 cut(s) 124, 728, 1386
CviAII CATG 3 cut(s) 292, 851, 1227
CviQI GTAC 3 cut(s) 124, 728, 1386
DdeI CTNAG 2 cut(s) 161, 198
DpnI GATC 4 cut(s) 384, 858, 995, 1186
DpnII GATC 4 cut(s) 382, 856, 993, 1184
Eam1104I CTCTTC 2 cut(s) 72, 422
EarI CTCTTC 2 cut(s) 72, 422
Eco130I CCWWGG 3 cut(s) 313, 344, 1209
Eco24I GRGCYC 1 cut(s) 47
Eco31I GGTCTC 1 cut(s) 784
Eco47I GGWCC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 377
EcoRI GAATTC 2 cut(s) 535, 894
EcoRII CCWGG 2 cut(s) 137, 1312
EcoT14I CCWWGG 3 cut(s) 313, 344, 1209
EcoT38I GRGCYC 1 cut(s) 47
ErhI CCWWGG 3 cut(s) 313, 344, 1209
FaeI CATG 3 cut(s) 295, 854, 1230
FalI AAGNNNNNCTT 2 cut(s) 553, 585
FatI CATG 3 cut(s) 291, 850, 1226
FauNDI CATATG 2 cut(s) 602, 1204
FokI GGATG 3 cut(s) 275, 709, 1181
FriOI GRGCYC 1 cut(s) 47
FspBI CTAG 6 cut(s) 345, 555, 623, 920, 1290, 1411
GlaI GCGC 2 cut(s) 159, 972
GsuI CTGGAG 1 cut(s) 597
HaeIII GGCC 1 cut(s) 782
HapII CCGG 6 cut(s) 371, 492, 783, 901, 932, 1123
HgaI GACGC 1 cut(s) 857
HhaI GCGC 2 cut(s) 160, 973
Hin1II CATG 3 cut(s) 295, 854, 1230
Hin6I GCGC 2 cut(s) 158, 971
HinP1I GCGC 2 cut(s) 158, 971
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 6 cut(s) 255, 451, 549, 577, 1028, 1136
HpaII CCGG 6 cut(s) 371, 492, 783, 901, 932, 1123
HphI GGTGA 2 cut(s) 804, 1328
Hpy188I TCNGA 9 cut(s) 201, 387, 396, 582, 679, 717, 1135, 1189, 1278
Hpy188III TCNNGA 6 cut(s) 71, 492, 614, 623, 997, 1140
HpyAV CCTTC 1 cut(s) 69
HpyCH4III ACNGT 3 cut(s) 915, 944, 979
HpyCH4IV ACGT 2 cut(s) 681, 1103
HpyCH4V TGCA 5 cut(s) 322, 650, 751, 1012, 1076
HpyF3I CTNAG 2 cut(s) 161, 198
HpySE526I ACGT 2 cut(s) 681, 1103
Hsp92II CATG 3 cut(s) 295, 854, 1230
HspAI GCGC 2 cut(s) 158, 971
Kpn2I TCCGGA 1 cut(s) 491
Kzo9I GATC 4 cut(s) 382, 856, 993, 1184
LmnI GCTCC 3 cut(s) 628, 1235, 1361
LweI GCATC 3 cut(s) 175, 378, 833
MabI ACCWGGT 2 cut(s) 137, 1312
MaeI CTAG 6 cut(s) 345, 555, 623, 920, 1290, 1411
MaeII ACGT 2 cut(s) 681, 1103
MaeIII GTNAC 4 cut(s) 482, 938, 1378, 1406
MalI GATC 4 cut(s) 384, 858, 995, 1186
MboI GATC 4 cut(s) 382, 856, 993, 1184
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 6 cut(s) 535, 589, 808, 894, 1302, 1390
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 896
MroI TCCGGA 1 cut(s) 491
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 2 cut(s) 660, 807
MslI CAYNNNNRTG 1 cut(s) 601
MspCI CTTAAG 1 cut(s) 659
MspI CCGG 6 cut(s) 371, 492, 783, 901, 932, 1123
MspR9I CCNGG 6 cut(s) 139, 371, 784, 932, 1124, 1314
Mva1269I GAATGC 1 cut(s) 324
MvaI CCWGG 2 cut(s) 139, 1314
MvnI CGCG 1 cut(s) 377
NciI CCSGG 4 cut(s) 371, 784, 932, 1124
NdeI CATATG 2 cut(s) 602, 1204
NdeII GATC 4 cut(s) 382, 856, 993, 1184
NlaIII CATG 3 cut(s) 295, 854, 1230
NlaIV GGNNCC 3 cut(s) 1059, 1128, 1357
NmuCI GTSAC 1 cut(s) 1406
NspI RCATGY 1 cut(s) 854
PaeI GCATGC 1 cut(s) 854
PaqCI CACCTGC 1 cut(s) 756
PctI GAATGC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 5 cut(s) 255, 451, 549, 1028, 1136
PfoI TCCNGGA 1 cut(s) 1122
PleI GAGTC 1 cut(s) 585
PpsI GAGTC 1 cut(s) 585
Psp6I CCWGG 2 cut(s) 137, 1312
PspGI CCWGG 2 cut(s) 137, 1312
PspN4I GGNNCC 3 cut(s) 1059, 1128, 1357
PspPI GGNCC 2 cut(s) 341, 780
PstI CTGCAG 1 cut(s) 652
RsaI GTAC 3 cut(s) 125, 729, 1387
RsaNI GTAC 3 cut(s) 124, 728, 1386
RseI CAYNNNNRTG 1 cut(s) 601
SaqAI TTAA 2 cut(s) 660, 807
Sau3AI GATC 4 cut(s) 382, 856, 993, 1184
Sau96I GGNCC 2 cut(s) 341, 780
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 6 cut(s) 139, 371, 784, 932, 1124, 1314
SduI GDGCHC 1 cut(s) 47
SexAI ACCWGGT 2 cut(s) 137, 1312
SfaNI GCATC 3 cut(s) 175, 378, 833
SfcI CTRYAG 2 cut(s) 648, 1032
SinI GGWCC 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
SphI GCATGC 1 cut(s) 854
Sse9I AATT 6 cut(s) 535, 589, 808, 894, 1302, 1390
SsiI CCGC 3 cut(s) 252, 377, 794
SspMI CTAG 6 cut(s) 345, 555, 623, 920, 1290, 1411
StyD4I CCNGG 6 cut(s) 137, 369, 782, 930, 1122, 1312
StyI CCWWGG 3 cut(s) 313, 344, 1209
TaaI ACNGT 3 cut(s) 915, 944, 979
TaiI ACGT 2 cut(s) 684, 1106
TaqI TCGA 3 cut(s) 425, 884, 996
TasI AATT 6 cut(s) 535, 589, 808, 894, 1302, 1390
TatI WGTACW 2 cut(s) 123, 1385
TfiI GAWTC 5 cut(s) 255, 451, 549, 1028, 1136
Tru1I TTAA 2 cut(s) 660, 807
Tru9I TTAA 2 cut(s) 660, 807
TseFI GTSAC 1 cut(s) 1406
Tsp45I GTSAC 1 cut(s) 1406
TspDTI ATGAA 3 cut(s) 589, 811, 1385
TspGWI ACGGA 2 cut(s) 275, 456
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 341
XapI RAATTY 3 cut(s) 535, 589, 894
XbaI TCTAGA 1 cut(s) 622
XceI RCATGY 1 cut(s) 854
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XmaJI CCTAGG 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 6 cut(s) 345, 555, 623, 920, 1290, 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.