RchiOBHm_Chr1g0358861

Mitogen-activated protein kinase kinase kinase 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013904
Physical Location & Seq
Forward (+)
50846240 .. 50847811
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58395

Sequence Viewer

Length: 1173 bp
ATGGAGCTGATGAGTAAAGACCTTGGCAGCTACATCAAGGAGATCTGTGGCCCAAGGAAGCGAATTCCATTTTCTCTTCCTGTTGCTATTGATTTGATGCTTCAAATTGCAAGAGGAATGGAATACCTCCACTCCAAGAAAATCTACCATGGAGAATTGAATCCTTGTAACATACTGGTCAAAGCAAGAGGAGTGTCCACAGATGGTTACTTGCTGGCAAAGGTTTCAGGTTTCGGCCTAACTTCTGTTAAAAACTCCACCCAGAAAAACCCTACAAATCAGAATGGAAGCCCACCTGTTATATGGTATTCTCCAGAAGTTCTGGAAGAGCAAGAACACAGTGAAAGTAGTGAGAAAAAGTATACAGAAAAGTCCGATGTGTATAGCTTTGCGATGGTTTGCTTTGAGCTTCTTACAGGTAAAGTGCCTTTTGAGGATAGTCATCTTCAAGGGGACAAAATGAGCCGAAATATTAGGGCAGGCGAGAGGCCATTGTTCCCCTTCTATTCTCTGAGATATGCCACCAACCTCACAAAGAGATGTTGGCATGCGGATCCAAATCAAAGGCCAAGCTTCTCATCCATCTGTAGGATTCTTCGCTATGTTAAAAGGTTCCTTGCCATGAATCCTGACTATGACAGCCAGCTAGACCAACCAGTGCATATGGTAGATTACTGTGACATCGAGTTGGGGGTTCTGAAAAAGTTCCCTTCTTGGGGGAGCTCTGATCAATTACCATTATGGCAAATCCCATTTCAGATGTTTGTTTATAGGATAGCGGAGAAAGTCAGAAACTCAGGCCTGAAAGATACTTCAGAATCAGGAAGCGACGCTTCGATATGTGGGGATGGAATTGTGACTCCACCAGATGATCCATTCCCAGCAATACCCGAAAGGAAGTGTTCACCTGATAGTACGAACAAGAAACTTCCCTTGTTGAGGAGATCTTCAGATGTGAAAGCCAACAGAGTACTAGGAACACCAAGAGGACGGTCTGCCAGACCTCCACAGGTAACAACCCCTTATCGTAGTATTAGCATGAAAATGAGTTCAGAAAGCCAGCAAAAGATGACTCAAAGAATACGGAGGACATCAACGAGTTCAGAAATGGTGATGAGTTCAAGAATACAGAGAACCAAATCCGGTCATGCCTCAGACTCTGAGCTCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

390

Amino Acids

44.1

Weight (kDa)

9.29

Isoelectric Point (pI)

59.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 196 1.8e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 21 - 198 2.4e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013904)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01450
fragaria_vesca FvH4_7g15980
malus_domestica MD01G1076300.v1.1 MD07G1145200.v1.1
prunus_persica Prupe.2G183700_v2.0.a1 Prupe.2G183700_v2.0.a1
pyrus_communis pycom07g14470
rosa_chinensis RchiOBHm_Chr1g0358861
rosa_laevigata RLG00000027921
rosa_multiflora Rmu_sc0008757.1_g000014
rosa_roxburghii Rroxscaffold_4G00297570
rosa_rugosa Rorug01G0265700
rosa_samantha Rh1AG279600 Rh1BG246400 Rh1CG263800 Rh1DG274800
rosa_wichuraiana Rw1G024860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 362
AciI CCGC 2 cut(s) 551, 779
AclWI GGATC 3 cut(s) 548, 561, 866
AcsI RAATTY 1 cut(s) 63
AcuI CTGAAG 2 cut(s) 798, 933
AfaI GTAC 2 cut(s) 916, 972
AfiI CCNNNNNNNGG 4 cut(s) 588, 715, 716, 939
AgsI TTSAA 4 cut(s) 104, 160, 449, 1122
AluBI AGCT 8 cut(s) 7, 30, 387, 409, 573, 646, 723, 1165
AluI AGCT 8 cut(s) 7, 30, 387, 409, 573, 646, 723, 1165
Alw21I GWGCWC 2 cut(s) 725, 1167
AlwI GGATC 3 cut(s) 548, 561, 866
AlwNI CAGNNNCTG 1 cut(s) 1160
AoxI GGCC 5 cut(s) 49, 235, 488, 566, 799
ApeKI GCWGC 1 cut(s) 27
ApoI RAATTY 1 cut(s) 63
Asp700I GAANNNNTTC 1 cut(s) 704
AspS9I GGNCC 1 cut(s) 50
AsuHPI GGTGA 2 cut(s) 897, 1123
BamHI GGATCC 1 cut(s) 553
BanII GRGCYC 2 cut(s) 725, 1167
Bbv12I GWGCWC 2 cut(s) 725, 1167
BbvI GCAGC 1 cut(s) 39
BccI CCATC 4 cut(s) 197, 388, 590, 842
BclI TGATCA 1 cut(s) 727
BfaI CTAG 3 cut(s) 647, 974, 1171
BfmI CTRYAG 1 cut(s) 586
BglII AGATCT 2 cut(s) 42, 944
BisI GCNGC 1 cut(s) 28
BlsI GCNGC 1 cut(s) 29
BmcAI AGTACT 1 cut(s) 972
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 2 cut(s) 555, 614
BmsI GCATC 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 297
BsaBI GATNNNNATC 2 cut(s) 441, 558
BsaJI CCNNGG 3 cut(s) 22, 53, 148
BsaWI WCCGGW 1 cut(s) 1142
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 4 cut(s) 588, 715, 716, 939
Bse1I ACTGG 2 cut(s) 180, 656
Bse8I GATNNNNATC 2 cut(s) 441, 558
BseDI CCNNGG 3 cut(s) 22, 53, 148
BseGI GGATG 2 cut(s) 578, 853
BseJI GATNNNNATC 2 cut(s) 441, 558
BseLI CCNNNNNNNGG 4 cut(s) 588, 715, 716, 939
BseMII CTCAG 4 cut(s) 503, 810, 1152, 1167
BseNI ACTGG 2 cut(s) 180, 656
BseRI GAGGAG 2 cut(s) 204, 955
BseXI GCAGC 1 cut(s) 39
BseYI CCCAGC 1 cut(s) 880
BshFI GGCC 5 cut(s) 51, 237, 490, 568, 801
BsiHKAI GWGCWC 2 cut(s) 725, 1167
BsiSI CCGG 1 cut(s) 1143
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 4 cut(s) 588, 715, 716, 939
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 5 cut(s) 51, 237, 490, 568, 801
Bsp1286I GDGCHC 2 cut(s) 725, 1167
Bsp143I GATC 5 cut(s) 42, 553, 727, 871, 944
Bsp19I CCATGG 1 cut(s) 148
BspACI CCGC 2 cut(s) 551, 779
BspANI GGCC 5 cut(s) 51, 237, 490, 568, 801
BspCNI CTCAG 4 cut(s) 504, 809, 1153, 1166
BspLI GGNNCC 2 cut(s) 555, 614
BspPI GGATC 3 cut(s) 548, 561, 866
BspQI GCTCTTC 1 cut(s) 321
BsrI ACTGG 2 cut(s) 180, 656
BssECI CCNNGG 3 cut(s) 22, 53, 148
BssMI GATC 5 cut(s) 42, 553, 727, 871, 944
BssNAI GTATAC 1 cut(s) 363
BssT1I CCWWGG 3 cut(s) 22, 53, 148
Bst1107I GTATAC 1 cut(s) 363
Bst4CI ACNGT 3 cut(s) 341, 677, 993
Bst6I CTCTTC 2 cut(s) 81, 321
BstC8I GCNNGC 5 cut(s) 216, 481, 549, 644, 1061
BstDEI CTNAG 4 cut(s) 512, 796, 1153, 1161
BstDSI CCRYGG 1 cut(s) 148
BstENI CCTNNNNNAGG 1 cut(s) 937
BstF5I GGATG 2 cut(s) 578, 853
BstKTI GATC 5 cut(s) 45, 556, 730, 874, 947
BstMBI GATC 5 cut(s) 42, 553, 727, 871, 944
BstNSI RCATGY 1 cut(s) 551
BstSFI CTRYAG 1 cut(s) 586
BstV1I GCAGC 1 cut(s) 39
BstX2I RGATCY 3 cut(s) 42, 553, 944
BstYI RGATCY 3 cut(s) 42, 553, 944
BstZ17I GTATAC 1 cut(s) 363
BsuRI GGCC 5 cut(s) 51, 237, 490, 568, 801
BtgI CCRYGG 1 cut(s) 148
BtgZI GCGATG 1 cut(s) 407
BtsCI GGATG 2 cut(s) 578, 853
BtsIMutI CAGTG 2 cut(s) 346, 663
Cac8I GCNNGC 5 cut(s) 216, 481, 549, 644, 1061
CaiI CAGNNNCTG 1 cut(s) 1160
Cfr13I GGNCC 1 cut(s) 50
CseI GACGC 1 cut(s) 839
Csp6I GTAC 2 cut(s) 915, 971
CviAII CATG 5 cut(s) 149, 548, 622, 1039, 1148
CviQI GTAC 2 cut(s) 915, 971
DdeI CTNAG 4 cut(s) 512, 796, 1153, 1161
DpnI GATC 5 cut(s) 44, 555, 729, 873, 946
DpnII GATC 5 cut(s) 42, 553, 727, 871, 944
Eam1104I CTCTTC 2 cut(s) 81, 321
EarI CTCTTC 2 cut(s) 81, 321
Ecl136II GAGCTC 2 cut(s) 723, 1165
Eco130I CCWWGG 3 cut(s) 22, 53, 148
Eco147I AGGCCT 1 cut(s) 801
Eco24I GRGCYC 2 cut(s) 725, 1167
Eco53kI GAGCTC 2 cut(s) 723, 1165
Eco57I CTGAAG 2 cut(s) 798, 933
EcoICRI GAGCTC 2 cut(s) 723, 1165
EcoNI CCTNNNNNAGG 1 cut(s) 937
EcoRI GAATTC 1 cut(s) 63
EcoT14I CCWWGG 3 cut(s) 22, 53, 148
EcoT38I GRGCYC 2 cut(s) 725, 1167
ErhI CCWWGG 3 cut(s) 22, 53, 148
FaeI CATG 5 cut(s) 152, 551, 625, 1042, 1151
FalI AAGNNNNNCTT 2 cut(s) 817, 849
FaqI GGGAC 1 cut(s) 467
FatI CATG 5 cut(s) 148, 547, 621, 1038, 1147
FauNDI CATATG 1 cut(s) 663
FbaI TGATCA 1 cut(s) 727
FblI GTMKAC 1 cut(s) 362
Fnu4HI GCNGC 1 cut(s) 28
FokI GGATG 2 cut(s) 565, 860
FriOI GRGCYC 2 cut(s) 725, 1167
Fsp4HI GCNGC 1 cut(s) 28
FspBI CTAG 3 cut(s) 647, 974, 1171
GluI GCNGC 1 cut(s) 28
GsaI CCCAGC 1 cut(s) 884
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 5 cut(s) 51, 237, 490, 568, 801
HapII CCGG 1 cut(s) 1143
HgaI GACGC 1 cut(s) 839
Hin1II CATG 5 cut(s) 152, 551, 625, 1042, 1151
HindIII AAGCTT 1 cut(s) 571
HinfI GANTC 7 cut(s) 160, 592, 625, 818, 859, 1072, 1157
HpaII CCGG 1 cut(s) 1143
HphI GGTGA 2 cut(s) 897, 1123
Hpy166II GTNNAC 3 cut(s) 198, 363, 905
Hpy188III TCNNGA 5 cut(s) 314, 323, 629, 822, 1122
Hpy8I GTNNAC 3 cut(s) 198, 363, 905
Hpy99I CGWCG 1 cut(s) 833
HpyAV CCTTC 2 cut(s) 511, 720
HpyCH4III ACNGT 3 cut(s) 341, 677, 993
HpyCH4V TGCA 2 cut(s) 110, 661
HpyF3I CTNAG 4 cut(s) 512, 796, 1153, 1161
Hsp92II CATG 5 cut(s) 152, 551, 625, 1042, 1151
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 5 cut(s) 42, 553, 727, 871, 944
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 2 cut(s) 4, 720
Lsp1109I GCAGC 1 cut(s) 39
LweI GCATC 1 cut(s) 87
MaeI CTAG 3 cut(s) 647, 974, 1171
MaeIII GTNAC 5 cut(s) 167, 206, 677, 856, 1012
MalI GATC 5 cut(s) 44, 555, 729, 873, 946
MboI GATC 5 cut(s) 42, 553, 727, 871, 944
MboII GAAGA 5 cut(s) 68, 338, 437, 587, 939
MflI RGATCY 3 cut(s) 42, 553, 944
MhlI GDGCHC 2 cut(s) 725, 1167
MluCI AATT 5 cut(s) 63, 105, 155, 731, 852
MlyI GAGTC 3 cut(s) 853, 1066, 1151
MroXI GAANNNNTTC 1 cut(s) 704
MseI TTAA 2 cut(s) 249, 606
MslI CAYNNNNRTG 1 cut(s) 1043
MspI CCGG 1 cut(s) 1143
NcoI CCATGG 1 cut(s) 148
NdeI CATATG 1 cut(s) 663
NdeII GATC 5 cut(s) 42, 553, 727, 871, 944
NlaIII CATG 5 cut(s) 152, 551, 625, 1042, 1151
NlaIV GGNNCC 2 cut(s) 555, 614
NmuCI GTSAC 2 cut(s) 677, 856
NspI RCATGY 1 cut(s) 551
PaeI GCATGC 1 cut(s) 551
PceI AGGCCT 1 cut(s) 801
PciSI GCTCTTC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 704
PfeI GAWTC 4 cut(s) 160, 592, 625, 818
PkrI GCNGC 1 cut(s) 29
PleI GAGTC 3 cut(s) 853, 1066, 1151
PpsI GAGTC 3 cut(s) 853, 1066, 1151
Psp124BI GAGCTC 2 cut(s) 725, 1167
PspFI CCCAGC 1 cut(s) 880
PspN4I GGNNCC 2 cut(s) 555, 614
PspPI GGNCC 1 cut(s) 50
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 3 cut(s) 42, 553, 944
RsaI GTAC 2 cut(s) 916, 972
RsaNI GTAC 2 cut(s) 915, 971
RseI CAYNNNNRTG 1 cut(s) 1043
SacI GAGCTC 2 cut(s) 725, 1167
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 2 cut(s) 249, 606
SatI GCNGC 1 cut(s) 28
Sau3AI GATC 5 cut(s) 42, 553, 727, 871, 944
Sau96I GGNCC 1 cut(s) 50
ScaI AGTACT 1 cut(s) 972
SchI GAGTC 3 cut(s) 853, 1066, 1151
SduI GDGCHC 2 cut(s) 725, 1167
SfaNI GCATC 1 cut(s) 87
SfcI CTRYAG 1 cut(s) 586
SmiMI CAYNNNNRTG 1 cut(s) 1043
SphI GCATGC 1 cut(s) 551
Sse9I AATT 5 cut(s) 63, 105, 155, 731, 852
SseBI AGGCCT 1 cut(s) 801
SsiI CCGC 2 cut(s) 551, 779
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 647, 974, 1171
SstI GAGCTC 2 cut(s) 725, 1167
StuI AGGCCT 1 cut(s) 801
StyI CCWWGG 3 cut(s) 22, 53, 148
TaaI ACNGT 3 cut(s) 341, 677, 993
TaqI TCGA 2 cut(s) 684, 836
TasI AATT 5 cut(s) 63, 105, 155, 731, 852
TatI WGTACW 1 cut(s) 970
TfiI GAWTC 4 cut(s) 160, 592, 625, 818
Tru1I TTAA 2 cut(s) 249, 606
Tru9I TTAA 2 cut(s) 249, 606
TscAI CASTG 2 cut(s) 346, 663
TseFI GTSAC 2 cut(s) 677, 856
TseI GCWGC 1 cut(s) 27
Tsp45I GTSAC 2 cut(s) 677, 856
TspDTI ATGAA 2 cut(s) 638, 1055
TspGWI ACGGA 1 cut(s) 1099
TspRI CASTG 2 cut(s) 346, 663
XagI CCTNNNNNAGG 1 cut(s) 937
XapI RAATTY 1 cut(s) 63
XceI RCATGY 1 cut(s) 551
XcmI CCANNNNNNNNNTGG 1 cut(s) 300
XmiI GTMKAC 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 704
XspI CTAG 3 cut(s) 647, 974, 1171
ZrmI AGTACT 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.