MD01G1076300.v1.1

Mitogen-activated protein kinase kinase kinase 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013904
Physical Location & Seq
Forward (+)
18223517 .. 18225526
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1076300.v1.1.491

Sequence Viewer

Length: 2010 bp
ATGGAGCAATTTCGAAAGATCGGGGAGGATTTAGGAAGTTTAAAGGCCTTAATGGTGTTCCAAGACAACATCCAAATCAATCAGCGGCAGTGCCTTCTGCTGCTCGATATCTTCAGCTCAGCGTACGAATCAATAGCGGAGGAGATGAGACATAATCTTCGGTTTGATGAGAAGCACATGAAATGGAGGGTTCTCGAGCAGCCGCTGAGAGAGCTCCACAGGATATTCAAAGAAGCAGAAGCTTACATTCGACAATGCTTGGAAACCAAGGATTGGTGGGCTAAGGCCATCACTCTCTATCAGAACTCCGATTGCGTTGAGTTTCACATCCATAACCTACTTGCCTGCATGCCAATCGTCATTGAAGCGATTGACATTGCTGGAGAAGTTTCAGGGTGGGATCAGGATGAGATACAGAGGAAGAAAACCGTGTTTGCGGATAAGTACAAAGAAGACTACAGAGATTGGAAACTTTTCAAGTGGAGGTTTGGGAAGCAGTATTTGATTACTCAAGACTTCTGCAATAGGTTTGAATCAGCAAGCAAAGAAGATAGATGGACTCTGCAGCATAAAATCCGCGAAAAGAAACTTTCGGGTTCAACAAAGTACGGGAAACGCCTCATAGATCTCCTTTTCAAAAGCATAGACGGATCAGAGACACAGAATTTGAATGGGAAGCTCTTACCTAGTTCAATCCTGGTGGGGTCTAAGGACTACCAGGTGAGGCGGCGACTTGGGGGTGGAAGTCAGTATAAGGAGATCCTATGGTTGGGTGAAAGCTTTGCCTCAAGACATTTAATTGGAGAAGTAGAAACCTTGTTGCCGGAGATTTCTTCGTTGTCATTGCTTTCCCACCCGAACATAGTGCACTTCCTTTGTGGGTTTACGGATGAGGAAAAGAAAGAGTGTTTTCTCATTATGGAACTCATGAGTAAAGACCTCTGCAGCTACATCAAAGAGATTTGTGGCCCGCGAAAACGTCTTCCATTTTCTCTTCCTGCTGCTATTGATCTGATGCTTCAAATTGCAAGAGGAATGGAATATCTCCACTCGAAGAAAATCTACCACGGAGATTTAAATCCTAGTAATATACTTGTTAAAGCAAGAGGCATCTCCACAGAAGGTTACTTACAAGCCAAGGTTTCAGGTTTCGGTTTAACTTCTGTCAAAAGCCCCACACAGAAAAGCACTTCAAATCAGAATGGGAGCCTACCTTTAATCTGGTATGCGCCGGAAGTTCTGGAAGAGCAAGAACATGGAAAAAGTACTGAGAAAAAGTACGCAGAAAAGTCCGATGTGTATAGCTTTGGGATGGTTTGCTTTGAGCTTCTGACAGGGAAAGTGCCCTTTGAGGACAGTCATCTCCAAGGGGACAAGATGAGCCGAAACATAAGGGCGGGGGAGAGGCCGCTGTTCCCATTCTACTCTCTTAGATATGTGACCAACCTCACAAAGAAATGTTGGCATAGTGATCCATACCAACGGCCAAGCTTCTCGTCCATCTGCAGGATTCTTCGCTATATCAAACGGTTCCTTGCAATGAATCCTGATTACAACAGCCAGCTAGACCCACCGGTTCCTATGGTAGATTACTGTGAGATTGAGTCAGTGCTTCTGAAAAAGATTCCTTCTTGGGGGACTTCTGAACCATCACCATTATCACAAATCCCATTCCAAATGTTCGTTTATAGGATCGCAGAGAGAGAAAGAACATCTTCAAGCCTGAAGGATACTTCAGAATCGGGAAGCGACGGAGCTTCAATCTGTGGGGACGAAATTGTGGTCCCTGCAGATGATTCGTTCCCATCAACACCTGAAAGGAAGAGTTTCTCTTCGCCTGATAGCGTGAAAAAGAAACTTCCATTCTTGAAGAAATCATCAGATGTGAAAGCCAACAGACTATCAGGTTGCCCTAACCACACAACTCATTTGTTCCTGATTATTTTCAAGACACAAATTAAACACCTACAACTTATTCACAATCATGGAAAAATAAGAAAAGGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1221 Pkinase_Tyr

Protein Analysis

670

Amino Acids

77.1

Weight (kDa)

8.71

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1221 PF06760 21 - 236 1.7e-103 Protein of unknown function (DUF1221)
PK_Tyr_Ser-Thr PF07714 270 - 504 1.7e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 271 - 502 1.1e-34 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013904)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01450
fragaria_vesca FvH4_7g15980
malus_domestica MD01G1076300.v1.1 MD07G1145200.v1.1
prunus_persica Prupe.2G183700_v2.0.a1 Prupe.2G183700_v2.0.a1
pyrus_communis pycom07g14470
rosa_chinensis RchiOBHm_Chr1g0358861
rosa_laevigata RLG00000027921
rosa_multiflora Rmu_sc0008757.1_g000014
rosa_roxburghii Rroxscaffold_4G00297570
rosa_rugosa Rorug01G0265700
rosa_samantha Rh1AG279600 Rh1BG246400 Rh1CG263800 Rh1DG274800
rosa_wichuraiana Rw1G024860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 273
AccII CGCG 2 cut(s) 579, 973
AciI CCGC 9 cut(s) 85, 137, 203, 437, 577, 727, 971, 1397, 1409
AclWI GGATC 5 cut(s) 408, 658, 754, 1466, 1700
AcoI YGGCCR 1 cut(s) 1484
AcsI RAATTY 1 cut(s) 664
AcuI CTGAAG 3 cut(s) 97, 1719, 1745
AfaI GTAC 5 cut(s) 125, 446, 608, 1267, 1280
AfiI CCNNNNNNNGG 4 cut(s) 273, 769, 1506, 1634
AgeI ACCGGT 1 cut(s) 1573
AjnI CCWGG 2 cut(s) 696, 717
Alw21I GWGCWC 2 cut(s) 216, 870
Alw26I GTCTC 2 cut(s) 142, 650
Alw44I GTGCAC 1 cut(s) 866
AlwI GGATC 5 cut(s) 408, 658, 754, 1466, 1700
AlwNI CAGNNNCTG 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 5 cut(s) 45, 285, 967, 1406, 1484
ApaLI GTGCAC 1 cut(s) 866
ApeKI GCWGC 5 cut(s) 100, 199, 565, 945, 1001
ApoI RAATTY 1 cut(s) 664
ArsI GACNNNNNNTTYG 6 cut(s) 141, 173, 649, 681, 1767, 1799
AsiGI ACCGGT 1 cut(s) 1573
Asp700I GAANNNNTTC 3 cut(s) 473, 1714, 1826
AspLEI GCGC 1 cut(s) 1231
AspS9I GGNCC 2 cut(s) 968, 1783
AsuHPI GGTGA 3 cut(s) 733, 785, 1644
AsuII TTCGAA 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 1783
BaeGI GKGCMC 2 cut(s) 870, 1347
BanII GRGCYC 1 cut(s) 216
BarI GAAGNNNNNNTAC 4 cut(s) 1046, 1078, 1113, 1145
BbsI GAAGAC 2 cut(s) 459, 974
Bbv12I GWGCWC 2 cut(s) 216, 870
BbvI GCAGC 5 cut(s) 87, 211, 577, 957, 988
BccI CCATC 6 cut(s) 296, 549, 1306, 1508, 1657, 1813
BceAI ACGGC 1 cut(s) 1499
BcgI CGANNNNNNTGC 6 cut(s) 337, 371, 847, 881, 1779, 1813
BciT130I CCWGG 2 cut(s) 698, 719
BciVI GTATCC 1 cut(s) 1723
BcoDI GTCTC 2 cut(s) 142, 650
BfaI CTAG 3 cut(s) 687, 1083, 1565
BfmI CTRYAG 5 cut(s) 457, 563, 943, 1504, 1788
BfuI GTATCC 1 cut(s) 1723
BglII AGATCT 1 cut(s) 625
BisI GCNGC 9 cut(s) 86, 101, 200, 203, 566, 728, 946, 1002, 1409
BlpI GCTNAGC 1 cut(s) 118
BlsI GCNGC 9 cut(s) 87, 102, 201, 204, 567, 729, 947, 1003, 1410
BmcAI AGTACT 1 cut(s) 1267
Bme1390I CCNGG 2 cut(s) 698, 719
Bme18I GGWCC 1 cut(s) 1783
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 2 cut(s) 968, 1783
BmiI GGNNCC 4 cut(s) 1208, 1532, 1578, 1785
BmrFI CCNGG 2 cut(s) 698, 719
BmsI GCATC 2 cut(s) 1005, 1119
BpiI GAAGAC 2 cut(s) 459, 974
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 118
Bpu14I TTCGAA 1 cut(s) 13
BpuEI CTTGAG 2 cut(s) 495, 772
BsaBI GATNNNNATC 1 cut(s) 1077
BsaJI CCNNGG 4 cut(s) 267, 1066, 1137, 1366
BsaWI WCCGGW 1 cut(s) 1573
Bsc4I CCNNNNNNNGG 4 cut(s) 273, 769, 1506, 1634
Bse118I RCCGGY 1 cut(s) 1573
Bse3DI GCAATG 3 cut(s) 375, 842, 1545
Bse8I GATNNNNATC 1 cut(s) 1077
BseBI CCWGG 2 cut(s) 698, 719
BseDI CCNNGG 4 cut(s) 267, 1066, 1137, 1366
BseGI GGATG 5 cut(s) 69, 327, 412, 895, 1317
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 4 cut(s) 273, 769, 1506, 1634
BseMI GCAATG 3 cut(s) 375, 842, 1545
BseMII CTCAG 3 cut(s) 132, 197, 1260
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 2 cut(s) 870, 1347
BseXI GCAGC 5 cut(s) 87, 211, 577, 957, 988
Bsh1236I CGCG 2 cut(s) 579, 973
BshFI GGCC 5 cut(s) 47, 287, 969, 1408, 1486
BshTI ACCGGT 1 cut(s) 1573
BsiHKAI GWGCWC 2 cut(s) 216, 870
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 3 cut(s) 824, 1232, 1574
BsiWI CGTACG 1 cut(s) 123
BslFI GGGAC 4 cut(s) 1385, 1651, 1769, 1784
BslI CCNNNNNNNGG 4 cut(s) 273, 769, 1506, 1634
BsmAI GTCTC 2 cut(s) 142, 650
BsmFI GGGAC 4 cut(s) 1385, 1651, 1769, 1784
BsnI GGCC 5 cut(s) 47, 287, 969, 1408, 1486
BsoBI CYCGRG 1 cut(s) 194
Bsp119I TTCGAA 1 cut(s) 13
Bsp1286I GDGCHC 3 cut(s) 216, 870, 1347
Bsp143I GATC 8 cut(s) 18, 400, 625, 650, 759, 1009, 1471, 1692
Bsp1720I GCTNAGC 1 cut(s) 118
BspACI CCGC 9 cut(s) 85, 137, 203, 437, 577, 727, 971, 1397, 1409
BspANI GGCC 5 cut(s) 47, 287, 969, 1408, 1486
BspCNI CTCAG 3 cut(s) 131, 198, 1261
BspFNI CGCG 2 cut(s) 579, 973
BspHI TCATGA 1 cut(s) 927
BspLI GGNNCC 4 cut(s) 1208, 1532, 1578, 1785
BspMAI CTGCAG 4 cut(s) 567, 947, 1508, 1792
BspPI GGATC 5 cut(s) 408, 658, 754, 1466, 1700
BspQI GCTCTTC 1 cut(s) 1239
BspT104I TTCGAA 1 cut(s) 13
BsrDI GCAATG 3 cut(s) 375, 842, 1545
BsrFI RCCGGY 1 cut(s) 1573
BssAI RCCGGY 1 cut(s) 1573
BssECI CCNNGG 4 cut(s) 267, 1066, 1137, 1366
BssMI GATC 8 cut(s) 18, 400, 625, 650, 759, 1009, 1471, 1692
BssT1I CCWWGG 3 cut(s) 267, 1137, 1366
Bst2UI CCWGG 2 cut(s) 698, 719
Bst4CI ACNGT 4 cut(s) 430, 1358, 1530, 1595
Bst6I CTCTTC 4 cut(s) 999, 1239, 1817, 1837
BstBI TTCGAA 1 cut(s) 13
BstC8I GCNNGC 5 cut(s) 346, 350, 541, 971, 1562
BstDEI CTNAG 6 cut(s) 118, 206, 282, 708, 1269, 1430
BstDSI CCRYGG 1 cut(s) 1066
BstF5I GGATG 5 cut(s) 69, 327, 412, 895, 1317
BstFNI CGCG 2 cut(s) 579, 973
BstHHI GCGC 1 cut(s) 1231
BstKTI GATC 8 cut(s) 21, 403, 628, 653, 762, 1012, 1474, 1695
BstMAI GTCTC 2 cut(s) 142, 650
BstMBI GATC 8 cut(s) 18, 400, 625, 650, 759, 1009, 1471, 1692
BstMWI GCNNNNNNNGC 1 cut(s) 211
BstNI CCWGG 2 cut(s) 698, 719
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 2 cut(s) 696, 717
BstSFI CTRYAG 5 cut(s) 457, 563, 943, 1504, 1788
BstSLI GKGCMC 2 cut(s) 870, 1347
BstUI CGCG 2 cut(s) 579, 973
BstV1I GCAGC 5 cut(s) 87, 211, 577, 957, 988
BstV2I GAAGAC 2 cut(s) 459, 974
BstX2I RGATCY 2 cut(s) 625, 759
BstYI RGATCY 2 cut(s) 625, 759
BsuI GTATCC 1 cut(s) 1723
BsuRI GGCC 5 cut(s) 47, 287, 969, 1408, 1486
BtgI CCRYGG 1 cut(s) 1066
BtsCI GGATG 5 cut(s) 69, 327, 412, 895, 1317
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 95, 1614
Cac8I GCNNGC 5 cut(s) 346, 350, 541, 971, 1562
CaiI CAGNNNCTG 1 cut(s) 205
CciI TCATGA 1 cut(s) 927
CfoI GCGC 1 cut(s) 1231
Cfr10I RCCGGY 1 cut(s) 1573
Cfr13I GGNCC 2 cut(s) 968, 1783
CsiI ACCWGGT 1 cut(s) 717
Csp6I GTAC 5 cut(s) 124, 445, 607, 1266, 1279
CspAI ACCGGT 1 cut(s) 1573
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviAII CATG 5 cut(s) 178, 349, 928, 1256, 1985
CviQI GTAC 5 cut(s) 124, 445, 607, 1266, 1279
DdeI CTNAG 6 cut(s) 118, 206, 282, 708, 1269, 1430
DpnI GATC 8 cut(s) 20, 402, 627, 652, 761, 1011, 1473, 1694
DpnII GATC 8 cut(s) 18, 400, 625, 650, 759, 1009, 1471, 1692
DraI TTTAAA 2 cut(s) 42, 1077
EaeI YGGCCR 1 cut(s) 1484
Eam1104I CTCTTC 4 cut(s) 999, 1239, 1817, 1837
EarI CTCTTC 4 cut(s) 999, 1239, 1817, 1837
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 3 cut(s) 267, 1137, 1366
Eco147I AGGCCT 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 216
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 1783
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 3 cut(s) 97, 1719, 1745
Eco88I CYCGRG 1 cut(s) 194
EcoICRI GAGCTC 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 696, 717
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 3 cut(s) 267, 1137, 1366
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 3 cut(s) 267, 1137, 1366
FaeI CATG 5 cut(s) 181, 352, 931, 1259, 1988
FalI AAGNNNNNCTT 2 cut(s) 1699, 1731
FaqI GGGAC 4 cut(s) 1385, 1651, 1769, 1784
FatI CATG 5 cut(s) 177, 348, 927, 1255, 1984
FauI CCCGC 2 cut(s) 978, 1390
Fnu4HI GCNGC 9 cut(s) 86, 101, 200, 203, 566, 728, 946, 1002, 1409
FokI GGATG 5 cut(s) 56, 314, 419, 902, 1324
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 9 cut(s) 86, 101, 200, 203, 566, 728, 946, 1002, 1409
FspBI CTAG 3 cut(s) 687, 1083, 1565
GlaI GCGC 1 cut(s) 1230
GluI GCNGC 9 cut(s) 86, 101, 200, 203, 566, 728, 946, 1002, 1409
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 5 cut(s) 47, 287, 969, 1408, 1486
HapII CCGG 3 cut(s) 824, 1232, 1574
HhaI GCGC 1 cut(s) 1231
Hin1II CATG 5 cut(s) 181, 352, 931, 1259, 1988
Hin6I GCGC 1 cut(s) 1229
HinP1I GCGC 1 cut(s) 1229
HindIII AAGCTT 3 cut(s) 240, 778, 1489
HinfI GANTC 9 cut(s) 128, 533, 559, 1510, 1543, 1604, 1624, 1739, 1796
HpaII CCGG 3 cut(s) 824, 1232, 1574
HphI GGTGA 3 cut(s) 733, 785, 1644
Hpy166II GTNNAC 2 cut(s) 868, 885
Hpy8I GTNNAC 2 cut(s) 868, 885
Hpy99I CGWCG 1 cut(s) 1754
HpyAV CCTTC 4 cut(s) 104, 1115, 1638, 1720
HpyCH4III ACNGT 4 cut(s) 430, 1358, 1530, 1595
HpyCH4IV ACGT 1 cut(s) 979
HpyCH4V TGCA 9 cut(s) 348, 522, 565, 868, 945, 1028, 1506, 1538, 1790
HpyF10VI GCNNNNNNNGC 1 cut(s) 211
HpyF3I CTNAG 6 cut(s) 118, 206, 282, 708, 1269, 1430
HpySE526I ACGT 1 cut(s) 979
Hsp92II CATG 5 cut(s) 181, 352, 931, 1259, 1988
HspAI GCGC 1 cut(s) 1229
Kzo9I GATC 8 cut(s) 18, 400, 625, 650, 759, 1009, 1471, 1692
LguI GCTCTTC 1 cut(s) 1239
LmnI GCTCC 4 cut(s) 4, 219, 1206, 1754
Lsp1109I GCAGC 5 cut(s) 87, 211, 577, 957, 988
LweI GCATC 2 cut(s) 1005, 1119
MabI ACCWGGT 1 cut(s) 717
MaeI CTAG 3 cut(s) 687, 1083, 1565
MaeII ACGT 1 cut(s) 979
MaeIII GTNAC 2 cut(s) 1124, 1438
MalI GATC 8 cut(s) 20, 402, 627, 652, 761, 1011, 1473, 1694
MboI GATC 8 cut(s) 18, 400, 625, 650, 759, 1009, 1471, 1692
MflI RGATCY 2 cut(s) 625, 759
MhlI GDGCHC 3 cut(s) 216, 870, 1347
MluCI AATT 6 cut(s) 8, 664, 798, 1023, 1776, 1956
MlyI GAGTC 2 cut(s) 553, 1613
MroXI GAANNNNTTC 3 cut(s) 473, 1714, 1826
MseI TTAA 8 cut(s) 41, 50, 797, 1076, 1098, 1157, 1217, 1959
MslI CAYNNNNRTG 1 cut(s) 1983
MspA1I CMGCKG 3 cut(s) 85, 205, 1411
MspI CCGG 3 cut(s) 824, 1232, 1574
MspR9I CCNGG 2 cut(s) 698, 719
MvaI CCWGG 2 cut(s) 698, 719
MvnI CGCG 2 cut(s) 579, 973
MwoI GCNNNNNNNGC 1 cut(s) 211
NdeII GATC 8 cut(s) 18, 400, 625, 650, 759, 1009, 1471, 1692
NlaIII CATG 5 cut(s) 181, 352, 931, 1259, 1988
NlaIV GGNNCC 4 cut(s) 1208, 1532, 1578, 1785
NmuCI GTSAC 1 cut(s) 1438
NspI RCATGY 1 cut(s) 352
NspV TTCGAA 1 cut(s) 13
PaeI GCATGC 1 cut(s) 352
PaeR7I CTCGAG 1 cut(s) 194
PagI TCATGA 1 cut(s) 927
PceI AGGCCT 1 cut(s) 47
PciSI GCTCTTC 1 cut(s) 1239
PdmI GAANNNNTTC 3 cut(s) 473, 1714, 1826
PfeI GAWTC 7 cut(s) 128, 533, 1510, 1543, 1624, 1739, 1796
Pfl23II CGTACG 1 cut(s) 123
PflMI CCANNNNNTGG 1 cut(s) 273
PinAI ACCGGT 1 cut(s) 1573
PkrI GCNGC 9 cut(s) 87, 102, 201, 204, 567, 729, 947, 1003, 1410
PleI GAGTC 2 cut(s) 553, 1612
PpsI GAGTC 2 cut(s) 553, 1612
Psp124BI GAGCTC 1 cut(s) 216
Psp6I CCWGG 2 cut(s) 696, 717
PspGI CCWGG 2 cut(s) 696, 717
PspLI CGTACG 1 cut(s) 123
PspN4I GGNNCC 4 cut(s) 1208, 1532, 1578, 1785
PspPI GGNCC 2 cut(s) 968, 1783
PstI CTGCAG 4 cut(s) 567, 947, 1508, 1792
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 2 cut(s) 625, 759
RsaI GTAC 5 cut(s) 125, 446, 608, 1267, 1280
RsaNI GTAC 5 cut(s) 124, 445, 607, 1266, 1279
RseI CAYNNNNRTG 1 cut(s) 1983
SacI GAGCTC 1 cut(s) 216
SapI GCTCTTC 1 cut(s) 1239
SaqAI TTAA 8 cut(s) 41, 50, 797, 1076, 1098, 1157, 1217, 1959
SatI GCNGC 9 cut(s) 86, 101, 200, 203, 566, 728, 946, 1002, 1409
Sau3AI GATC 8 cut(s) 18, 400, 625, 650, 759, 1009, 1471, 1692
Sau96I GGNCC 2 cut(s) 968, 1783
ScaI AGTACT 1 cut(s) 1267
SchI GAGTC 2 cut(s) 553, 1613
ScrFI CCNGG 2 cut(s) 698, 719
SduI GDGCHC 3 cut(s) 216, 870, 1347
SexAI ACCWGGT 1 cut(s) 717
SfaNI GCATC 2 cut(s) 1005, 1119
SfcI CTRYAG 5 cut(s) 457, 563, 943, 1504, 1788
Sfr274I CTCGAG 1 cut(s) 194
SfuI TTCGAA 1 cut(s) 13
SinI GGWCC 1 cut(s) 1783
SlaI CTCGAG 1 cut(s) 194
SmiI ATTTAAAT 1 cut(s) 1077
SmiMI CAYNNNNRTG 1 cut(s) 1983
SmlI CTYRAG 3 cut(s) 194, 510, 787
SmoI CTYRAG 3 cut(s) 194, 510, 787
SphI GCATGC 1 cut(s) 352
Sse9I AATT 6 cut(s) 8, 664, 798, 1023, 1776, 1956
SseBI AGGCCT 1 cut(s) 47
SsiI CCGC 9 cut(s) 85, 137, 203, 437, 577, 727, 971, 1397, 1409
SspMI CTAG 3 cut(s) 687, 1083, 1565
SstI GAGCTC 1 cut(s) 216
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 2 cut(s) 696, 717
StyI CCWWGG 3 cut(s) 267, 1137, 1366
SwaI ATTTAAAT 1 cut(s) 1077
TaaI ACNGT 4 cut(s) 430, 1358, 1530, 1595
TaiI ACGT 1 cut(s) 982
TaqI TCGA 5 cut(s) 13, 105, 195, 250, 1052
TasI AATT 6 cut(s) 8, 664, 798, 1023, 1776, 1956
TatI WGTACW 2 cut(s) 444, 1265
TauI GCSGC 4 cut(s) 88, 205, 730, 1411
TfiI GAWTC 7 cut(s) 128, 533, 1510, 1543, 1624, 1739, 1796
Tru1I TTAA 8 cut(s) 41, 50, 797, 1076, 1098, 1157, 1217, 1959
Tru9I TTAA 8 cut(s) 41, 50, 797, 1076, 1098, 1157, 1217, 1959
TscAI CASTG 2 cut(s) 95, 1614
TseFI GTSAC 1 cut(s) 1438
TseI GCWGC 5 cut(s) 100, 199, 565, 945, 1001
Tsp45I GTSAC 1 cut(s) 1438
TspDTI ATGAA 2 cut(s) 194, 1556
TspGWI ACGGA 4 cut(s) 663, 902, 1083, 1767
TspRI CASTG 2 cut(s) 95, 1614
Van91I CCANNNNNTGG 1 cut(s) 273
VneI GTGCAC 1 cut(s) 866
VpaK11BI GGWCC 1 cut(s) 1783
XapI RAATTY 1 cut(s) 664
XceI RCATGY 1 cut(s) 352
XhoI CTCGAG 1 cut(s) 194
XmnI GAANNNNTTC 3 cut(s) 473, 1714, 1826
XspI CTAG 3 cut(s) 687, 1083, 1565
ZrmI AGTACT 1 cut(s) 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.